Intestinal flora sampling device and method based on neonatal septicemia research

Abstract

The invention relates to an intestinal flora sampling device and method based on neonatal septicemia research, the intestinal flora sampling device comprises a sampling table and a sampling barrel fixed on the sampling table, the sampling barrel is provided with a passing opening penetrating through the sampling barrel, the passing opening is internally provided with a bearing film through a retractable assembly, a sampling mechanism is installed in the sampling table, and the sampling mechanism is connected with the bearing film. The sampling mechanism is used for dipping and collecting excrement, the cleaning mechanism is mounted in the sampling barrel and is used for cleaning the excrement splashed to the inner wall of the sampling barrel, excrement discharge grooves are formed in the bearing film at equal intervals in the length direction, and the distance between the two excrement discharge grooves is larger than the length of a single excrement discharge groove. The bearing film is conveyed through the collecting and releasing assembly, so that the excrement does not make contact with water when falling into the sampling barrel, the concentration of the excrement is ensured, and the accuracy of a detection result is ensured.

Description

Technical Field

[0001] The present invention relates to the field of stool sampling, and in particular to an intestinal flora sampling device and method based on neonatal sepsis research. Background Art

[0002] Late-onset sepsis in premature infants has an insidious onset. Detecting the intestinal flora of premature infants after birth through high-throughput sequencing (16srDNA) technology provides a clinical basis for the precise prevention and treatment of sepsis. It can identify high-risk children early and carry out active prevention and care, thereby reducing complications and mortality.

[0003] After premature infants are admitted to the hospital, stool samples are collected on days 1, 4, and 7 after birth, and then weekly thereafter. The endpoint for stool sampling is the onset of sepsis, discharge, or the fourth week after birth (whichever comes first). Stool sampling is convenient and reduces iatrogenic damage to the infant from repeated blood draws.

[0004] When sampling, the sampler needs to wear sterile gloves and use a sterile cotton swab to collect fresh stool. When collecting, care must be taken to avoid mixing feces and urine, and aseptic operation must be maintained. The sample should be divided into three 1.5mL sterile, enzyme-free EP tubes and stored in a -80°C refrigerator within 2 hours. After the sample is collected, it should be quickly transported to the laboratory on dry ice for high-throughput sequencing (16S rRNA) analysis. This operation process is relatively cumbersome.

[0005] The specimen is the child's stool, which is convenient for sampling and reduces the iatrogenic damage to the child caused by repeated blood draws.

[0006] A medical stool sampling device, a toilet device, and a stool sampling method were disclosed after searching, with announcement number: 202011382934.8;

[0007] This device is intended for patients who can move independently and cannot be used on infants and young children;

[0008] The device actually combines the sampling mechanism with the toilet. This method easily causes feces to combine with water. The water content of infant feces is already high, and when mixed with water, the concentration of the sample is too low, which in turn affects the test results. Summary of the Invention

[0009] The purpose of the present invention is to provide an intestinal flora sampling device and method based on neonatal sepsis research to solve the problems raised in the above background technology.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] An intestinal flora sampling device based on neonatal sepsis research, comprising:

[0012] A sampling platform and a sampling barrel fixed on the sampling platform, wherein the sampling barrel is provided with a through opening penetrating the sampling barrel, and a carrying film is installed in the through opening via a retractable assembly;

[0013] A sampling mechanism is installed in the sampling platform, and stool is dipped and collected by the sampling mechanism;

[0014] A cleaning mechanism is installed in the sampling barrel, and is used to clean feces splashed onto the inner wall of the sampling barrel;

[0015] Wherein, defecation grooves are equidistantly provided on the carrier film along the length direction, and the distance between two defecation grooves is greater than the length of a single defecation groove.

[0016] As a further solution of the present invention: the retracting assembly includes an unwinding roller and a rewinding roller rotatably mounted in the sampling table and located at both ends of the sampling table;

[0017] A plurality of limiting rollers are also rotatably installed in the sampling platform;

[0018] A No. 1 motor is fixed in the sampling platform, and the No. 1 motor is connected to the winding roller via a belt;

[0019] A flattening piece is installed in the sampling platform.

[0020] As a further solution of the present invention: the flattening member includes two sets of rolling members rotatably mounted on both sides of the carrier film;

[0021] The rolling element includes two tension rollers, which are respectively located at the top and bottom of the carrier film;

[0022] One side of each of the two tensioning rollers is rotatably mounted with a No. 1 gear set, the No. 1 gear set comprising two mutually meshing gears, the two gears being connected to the two No. 1 gear sets respectively through two No. 1 synchronous belts;

[0023] A second gear set is rotatably mounted between the two rolling elements. The two second gear sets include two gears meshing with each other. The two gears are respectively connected to one of the first gear sets in the two rolling elements through a second synchronous belt.

[0024] A No. 2 motor is also fixed to one side of the No. 1 gear set, and the No. 2 motor is connected to one of the No. 1 gear sets via a belt.

[0025] As a further solution of the present invention: the sampling mechanism includes three supporting members fixed in the sampling platform, a carrying ring is rotatably mounted between the three supporting members, and the carrying ring passes through the sampling barrel and is in sealing and sliding connection with the sampling barrel;

[0026] The bottom circumference of the carrying ring is equidistantly provided with a plurality of assembly grooves, each of which is equipped with a dipping assembly, and the sampling station is also equipped with a sealing tube assembly that cooperates with the dipping assembly;

[0027] A transmission wheel is rotatably mounted on each of the two supporting members, and the transmission wheel and the carrying ring are in rolling cooperation. A No. 1 motor is fixed on each of the two supporting members, and the output shaft of the No. 1 motor is coaxially fixed with the transmission wheel.

[0028] As a further solution of the present invention: the dipping assembly includes an electric push rod No. 1 fixed in the assembly groove, the movable rod end of the electric push rod No. 1 is coaxially fixed with a mounting portion, a plurality of electromagnetic sliders for clamping are fixed in the mounting portion, and a sampling piece is detachably installed in the electromagnetic slider.

[0029] As a further solution of the present invention: the tube sealing assembly includes a delivery member fixed in the sampling platform, the delivery member is provided with a tube supply port on the top, the tube supply port cooperates with the assembly groove, and an electric push rod is fixed to the bottom of the delivery member, the electric push rod is located directly below the tube supply port, and the movable rod of the electric push rod passes through the bottom of the delivery member;

[0030] A screw is rotatably installed on one side of the delivery member, and a threaded sleeve is sleeved on the screw to cooperate with the screw, and the threaded sleeve is fixed to a push plate slidably installed in the delivery member. A No. 2 motor is also fixed on the assembly groove, and the output shaft of the No. 2 motor is coaxially fixed with the screw.

[0031] As a further solution of the present invention: the sealing tube assembly further comprises a protective shell, the top of the assembly groove is provided with a top hole, and the diameter of the top hole is larger than that of the sampling piece;

[0032] A sealing cover matched with the top hole is fixed on the rod body of the sampling piece.

[0033] As a further solution of the present invention: the cleaning mechanism includes a plurality of through ports fixed in the sampling barrel;

[0034] A blocking cover for blocking the sampling barrel is also slidably mounted on the sampling platform. An integrated nozzle is provided at the bottom of the blocking cover. Both the integrated nozzle and the blocking cover are connected to a water supply device.

[0035] As a further solution of the present invention: pressure strips are slidably installed on both sides of the sampling barrel, and a No. 2 electric push rod is fixed on both sides of the two sampling barrels, and the movable rod of the No. 2 electric push rod is fixed to the pressure strip.

[0036] The present invention also provides a method for sampling intestinal flora based on neonatal sepsis research, which uses the intestinal flora sampling device based on neonatal sepsis research, comprising the following steps:

[0037] Step 1: The infant lies on the sampling table with his or her buttocks in the sampling bucket, so that the feces of the infant falls into the sampling bucket during defecation;

[0038] Step 2: When the stool falls into the sampling bucket, it will be caught by the supporting film, so that the stool falls onto the supporting film;

[0039] Step three: sampling the stool on the supporting film through the sampling mechanism. After the stool sampling is completed, the sampling barrel is cleaned through the cleaning mechanism.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] 1. The present invention transports the carrying film through the retractable assembly, so that the feces will not come into contact with water when falling into the sampling bucket, thereby ensuring the concentration of the feces and the accuracy of the test results;

[0042] Second, the present invention can sample and preserve different infants and young children separately through the sampling mechanism, and the structure is relatively simple;

[0043] Third, the present invention can be cleaned after each sampling work is completed, and the carrier film is also replaced during transportation to avoid repeated sampling of feces;

[0044] Fourth, the present invention has a relatively high degree of automation, can automatically achieve the above effects, and is relatively practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 This is a schematic diagram of the overall structure of the intestinal flora sampling device based on neonatal sepsis research from an upward perspective.

[0046] Figure 2 This is a schematic diagram of the overall top view of the intestinal flora sampling device based on neonatal sepsis research.

[0047] Figure 3 A cross-sectional view of a gut microbiota sampling device used in neonatal sepsis research.

[0048] Figure 4 Schematic diagram of the internal structure of the intestinal flora sampling device based on neonatal sepsis research.

[0049] Figure 5 Schematic diagram of the flattening component in the intestinal flora sampling device based on neonatal sepsis research.

[0050] Figure 6This is a schematic diagram of the structure of the layering strip and No. 2 electric push rod in the intestinal flora sampling device based on neonatal sepsis research.

[0051] Figure 7 Schematic diagram of the structure of the scraper in the intestinal flora sampling device based on neonatal sepsis research.

[0052] Figure 8 Schematic diagram of the cleaning mechanism in the intestinal flora sampling device based on neonatal sepsis research.

[0053] Figure 9 Schematic diagram of the location of the sampling mechanism of the intestinal flora sampling device based on neonatal sepsis research.

[0054] Figure 10 This is a schematic diagram of the upward-looking structure of the sampling mechanism in the intestinal flora sampling device based on neonatal sepsis research.

[0055] Figure 11 This is a schematic diagram of the top view of the sampling components in the intestinal flora sampling mechanism based on neonatal sepsis research.

[0056] Figure 12 This is a schematic diagram of the top view of the dipping component in the intestinal flora sampling device based on neonatal sepsis research.

[0057] Figure 13 This is a schematic diagram of the top view of the dipping component in the intestinal flora sampling device based on neonatal sepsis research.

[0058] Figure 14 for Figure 12 A magnified view of the local structure at point A.

[0059] Figure 15 for Figure 13 A magnified view of the local structure at point B in the middle.

[0060] Figure 16 This is a schematic diagram of the structure of the delivery components of the intestinal flora sampling device based on neonatal sepsis research.

[0061] Figure 17 Schematic diagram of the structure of the integrated nozzle in the intestinal flora sampling device based on neonatal sepsis research.

[0062] Figure: 1. Sampling station; 101. Sampling barrel; 102. Integrated nozzle; 103. Through port; 104. Scraper; 105. Sealing cap; 2. Carrying ring; 201. Drive wheel; 202. Support member; 203. Assembly slot; 204. Dispensing member; 205. Motor No. 1; 206. Screw; 207. Feeding piece; 208. Motor No. 2; 209. Electric push rod No. 1; 2010. Mounting unit; 2011. Electromagnetic slider; 2012, sampling piece; 2013, protective shell; 2014, top hole; 2015, sealing cover; 3, carrying film; 301, defecation trough; 4, unwinding roller; 401, limiting roller; 402, winding roller; 403, No. 1 motor; 5, tensioning roller; 501, No. 1 gear set; 502, No. 1 synchronous belt; 503, No. 2 gear set; 504, No. 2 synchronous belt; 505, No. 2 motor; 6, pressure strip; 601, No. 2 electric push rod. DETAILED DESCRIPTION

[0063] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0064] In addition, when an element in the present invention is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be an intermediate element. When an element is referred to as being "connected to" another element, it may be directly connected to the other element or there may be an intermediate element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0065] For example 1, please refer to Figures 1 to 17 A device for sampling intestinal flora based on neonatal sepsis research includes a sampling platform 1 and a sampling bucket 101 fixed on the sampling platform 1. The sampling bucket 101 is provided with a through-port 103 penetrating the sampling bucket 101. A carrying film 3 is installed in the through-port 103 via a retractable assembly.

[0066] A sampling mechanism is installed in the sampling platform 1, and stool is dipped and collected through the sampling mechanism;

[0067] A cleaning mechanism is installed in the sampling barrel 101, and is used to clean feces splashed onto the inner wall of the sampling barrel 101;

[0068] The supporting film 3 is provided with defecation grooves 301 at equal intervals along the length direction, and the distance between two defecation grooves 301 is greater than the length of a single defecation groove 301 .

[0069] In the embodiment of the present invention, the infant lies on the sampling table 1 with its buttocks at the sampling bucket 101, so that the feces of the infant falls into the sampling bucket 101 during defecation;

[0070] When feces falls into the sampling bucket 101, it will be received by the supporting film 3, so that the feces fall onto the supporting film 3. Then, the sampling mechanism will sample the feces on the supporting film 3. After the feces sampling is completed, the sampling bucket 101 will be cleaned by the cleaning mechanism.

[0071] The retracting assembly includes an unwinding roller 4 and a rewinding roller 402 rotatably mounted in the sampling platform 1 and located at both ends of the sampling platform 1;

[0072] A plurality of limiting rollers 401 are also rotatably installed in the sampling platform 1;

[0073] A No. 1 motor 403 is fixed in the sampling platform 1, and the No. 1 motor 403 is connected to the winding roller 402 via a belt;

[0074] A flattening piece is installed in the sampling platform 1 .

[0075] In the embodiment of the present invention, in the initial state, the carrier film 3 is in a rolled-up shape and wound onto the unwinding roller 4. One end of the carrier film 3 is pulled through the plurality of limiting rollers 401, then through the flattening member and the through-port 103, and finally fixed to the winding roller 402.

[0076] The function of the plurality of limiting rollers 401 is to limit and guide the carrier film 3 to prevent the carrier film 3 from being excessively rubbed and broken.

[0077] When the first motor 403 is working, the winding roller 402 is driven to rotate through the belt, and the carrier film 3 is wound when the winding roller 402 rotates, so that a pulling force is generated on the carrier film 3 when the winding roller 402 is wound, so that the unwinding roller 4 can unwind the carrier film, thereby conveying the carrier film 3;

[0078] Among them, the No. 1 motor 403 in the present invention adopts a motor with a built-in worm gear. Of course, other high-torque motors can also be used as equivalent replacements. At the same time, the belt is equivalently replaced by a chain. The present invention does not make specific restrictions and can be selected arbitrarily to meet the needs.

[0079] The flattening member includes two sets of rolling members rotatably mounted on both sides of the carrier film 3;

[0080] The rolling element includes two tension rollers 5, which are respectively located at the top and bottom of the carrier film 3;

[0081] A number one gear set 501 is rotatably mounted on one side of each of the two tension rollers 5. The number one gear set 501 includes two mutually meshing gears, and the two gears are connected to the two number one gear sets 501 through two number one synchronous belts 502.

[0082] A second gear set 503 is rotatably mounted between the two rolling elements. The two second gear sets 503 include two mutually meshing gears. The two gears are respectively connected to one of the first gear sets 501 of the two rolling elements via a second timing belt 504.

[0083] A second motor 505 is also fixed to one side of the first gear set 501 , and the second motor 505 is connected to one of the first gear sets 501 via a belt.

[0084] In the embodiment of the present invention, the two groups of rolling elements include a first tensioning roller 5, a second tensioning roller 5, a third tensioning roller 5, and a fourth tensioning roller 5. The first tensioning roller 5 and the second tensioning roller 5 form a group, and the third tensioning roller 5 and the fourth tensioning roller 5 form a group.

[0085] The first gear set 501 includes a first gear and a second gear, and the first gear and the second gear are respectively connected to the first tensioning roller 5 and the second tensioning roller 5 through a first synchronous belt 502 and connected to the third tensioning roller 5 and the fourth tensioning roller 5 through another first gear set 501;

[0086] The second gear set 503 includes a third gear and a fourth gear that mesh with each other. The third gear and the fourth gear are connected to the first tensioning roller 5 and the third tensioning roller 5 respectively through the second synchronous belt 504;

[0087] When the No. 2 motor 505 is working, its conveying shaft drives one of the No. 1 gear sets 501 to rotate through the belt, so that when the No. 1 gear set 501 rotates, the third tensioning roller 5 and the fourth tensioning roller 5 are driven to rotate relative to each other through the No. 1 synchronous belt 502. At the same time, the No. 2 gear set 503 is driven to rotate through the No. 2 synchronous belt 504, so that when the No. 2 gear set 503 rotates, the other No. 1 gear set 501 is driven to drive the first tensioning roller 5 and the second tensioning roller 5 to rotate relative to each other. Due to the existence of the No. 2 gear set 503, the first tensioning roller 5 and the third tensioning roller 5 also rotate relative to each other.

[0088] Then, the four tension rollers 5 pull the carrier film 3 to avoid wrinkles.

[0089] The second embodiment is distinguished from the first embodiment in that the sampling mechanism includes three supporting members 202 fixed in the sampling platform 1, a carrying ring 2 is rotatably mounted between the three supporting members 202, and the carrying ring 2 passes through the sampling barrel 101 and is in sealing and sliding connection therewith;

[0090] The bottom circumference of the carrying ring 2 is equidistantly provided with a plurality of assembly grooves 203, each of which is equipped with a dipping assembly. The sampling station 1 is also equipped with a sealing tube assembly that cooperates with the dipping assembly.

[0091] A transmission wheel 201 is rotatably mounted on the two relative support members 202 , and the transmission wheel 201 is in rolling cooperation with the carrying ring 2 . A No. 1 motor 205 is fixed on each of the two support members 202 , and the output shaft of the No. 1 motor 205 is coaxially fixed with the transmission wheel 201 .

[0092] In this embodiment of the present invention, when the first motor 205 is operating, its output shaft drives the transmission wheel 201 to rotate, and when the transmission wheel 201 rotates, the carrier ring 2 is driven to rotate. The purpose of providing two transmission wheels 201 is that, because the carrier ring 2 is in an unfinished state and has gaps, the two transmission wheels 201 can rotate the carrier ring 2 at any angle and number of revolutions without losing power transmission with the transmission wheel 201.

[0093] By rotating the supporting ring 2, any assembly groove 203 can be placed in the sampling barrel 101, and each sampling barrel 101 is equipped with a dipping assembly. When the corresponding dipping assembly is in the sampling barrel 101, the feces on the supporting film 3 can be dipped by the dipping assembly, and after dipping, the feces can be sealed and preserved by the sealing tube assembly.

[0094] The dipping assembly includes an electric push rod 209 fixed in the assembly groove 203, and a mounting portion 2010 is coaxially fixed to the movable rod end of the electric push rod 209. A plurality of electromagnetic sliders 2011 for clamping are fixed in the mounting portion 2010, and a sampling piece 2012 is detachably installed in the electromagnetic slider 2011.

[0095] In the embodiment of the present invention, when the corresponding assembly slot 203 is in the sampling barrel 101, the designated No. 1 electric push rod 209 is driven to work. When the No. 1 electric push rod 209 works, the movable rod is extended, thereby driving the sampling member 2012 to stand upright and descend, thereby dipping the feces on the carrier film 3, and then retracting it into the assembly slot 203;

[0096] Among them, by rotating the carrying ring 2, any assembly groove 203 can be placed in the sampling barrel 101, and then all the dipping components can complete the sampling work;

[0097] The movement of the plurality of electromagnetic sliders 2011 is based on the push of the electromagnet to achieve the clamping of the sampling piece 2012. Of course, the clamping of the sampling piece 2012 can also be released, and the process is changed to the above-mentioned detachable installation.

[0098] The tube sealing assembly includes a delivery member 204 fixed in the sampling platform 1, a tube supply port is provided on the top of the delivery member 204, and the tube supply port cooperates with the assembly groove 203. An electric push rod is also fixed to the bottom of the delivery member 204, and the electric push rod is located directly below the tube supply port. The movable rod of the electric push rod passes through the bottom of the delivery member 204;

[0099] A screw rod 206 is rotatably installed on one side of the delivery member 204, and a threaded sleeve is sleeved on the screw rod 206 and threadedly matched with it. The threaded sleeve is fixed to the push plate slidably installed in the delivery member 204. A No. 2 motor 208 is also fixed on the assembly groove 203, and the output shaft of the No. 2 motor 208 is coaxially fixed with the screw rod 206.

[0100] In an embodiment of the present invention, when the No. 2 motor 208 is working, the output shaft thereof drives the screw rod 206 to rotate, so that when the screw rod 206 rotates, the supporting piece 207 is driven to slide in the delivery member 204 through cooperation with the threaded sleeve, so that when the protective shell 2013 is stored in the delivery member 204, the protective shell 2013 is pushed to the position of the pipe supply port. When the protective shell 2013 is at the position of the pipe supply port, the protective shell 2013 can be pushed upright and raised by the electric push rod, so that the protective shell 2013 is inserted into the assembly slot 203. When the protective shell 2013 is inserted into the assembly slot 203, it is an interference fit relationship, and the protective shell 2013 will not fall off.

[0101] The sealing tube assembly further includes a protective shell 2013 , and a top hole 2014 is formed on the top of the assembly slot 203 . The diameter of the top hole 2014 is larger than that of the sampling piece 2012 .

[0102] A sealing cover 2015 that matches the top hole 2014 is fixed on the rod body of the sampling member 2012 .

[0103] In the embodiment of the present invention, when the protective shell 2013 is pushed to the end of the assembly groove 203, it abuts against the mounting portion 2010, thereby allowing the sampling piece 2012 to be inserted into the protective shell 2013 through the top hole 2014, and at the same time, the cover 2015 blocks the top hole 2014. There is also an interference fit relationship between the top hole 2014 and the cover 2015. When the cover 2015 is stuck on the top hole 2014, the clamping of the sampling piece 2012 can be released. At this time, the sampling piece 2012 and the protective shell 2013 are a complete storage piece. At this time, the protective shell 2013 and the sampling piece 2012 are a whole.

[0104] When the protective shell 2013 and the sampling piece 2012 need to be taken out, the No. 1 electric push rod 209 can be directly driven to work, and the protective shell 2013 can be pushed out through the No. 1 electric push rod 209.

[0105] The third embodiment is distinguished from the first embodiment and / or the second embodiment in that: the cleaning mechanism includes a plurality of through ports 103 fixed in the sampling barrel 101;

[0106] A blocking cover 105 for blocking the sampling barrel 101 is also slidably mounted on the sampling platform 1 . An integrated nozzle 102 is provided at the bottom of the blocking cover 105 . Both the integrated nozzle 102 and the blocking cover 105 are connected to a water supply device.

[0107] In an embodiment of the present invention, the sampling barrel 101 can be sealed when the sealing cover 105 is moved to the end of the stroke by an electric slide rail or manually driven. At the same time, water is supplied by the integrated nozzle 102 and the sealing cover 105 through the water supply equipment to clean the inside of the sampling barrel 101 by spraying water.

[0108] A pressure strip 6 is slidably mounted on both sides of the sampling barrel 101 , and a second electric push rod 601 is fixed on both sides of the two sampling barrels 101 , and the movable rod of the second electric push rod 601 is fixed to the pressure strip 6 .

[0109] In the embodiment of the present invention, the second electric push rod 601 is used to drive the holding bar 6 to move vertically and vertically. When the holding bar 6 descends, it presses both sides of the carrier film 3. Since the carrier film 3 is in a taut state, in order to prevent the carrier film 3 from being twisted when receiving the edge banding, the two ends of the carrier film 3 are fixed by pressing the two holding bars 6.

[0110] Among them, since the defecation grooves 301 on the supporting film 3 are arranged at equal intervals and the distance between the two defecation grooves 301 is greater than the length of the supporting film 3, the parts without defecation grooves 301 are respectively received, and the positions of the defecation grooves 301 will follow the transportation of the supporting film 3, and cooperate with the scraper 104 in the sampling bucket 101 to scrape off the feces, so that the feces fall into the sampling bucket 101.

[0111] The present invention also provides a method for sampling intestinal flora based on neonatal sepsis research, which uses the intestinal flora sampling device based on neonatal sepsis research, comprising the following steps:

[0112] Step 1: The infant lies on the sampling table 1 with its buttocks positioned in the sampling bucket 101 so that feces fall into the sampling bucket 101 during defecation.

[0113] Step 2: When the stool falls into the sampling bucket 101, it will be received by the supporting film 3, so that the stool falls onto the supporting film 3;

[0114] Step three: sampling the stool on the supporting film 3 by the sampling mechanism. After the stool sampling is completed, the sampling barrel 101 is cleaned by the cleaning mechanism.

[0115] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

[0116] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A device for sampling intestinal flora based on neonatal sepsis research, characterized in that: include: A sampling platform (1) and a sampling barrel (101) fixed on the sampling platform (1), wherein the sampling barrel (101) is provided with a through opening (103) penetrating the sampling barrel (101), and a carrying film (3) is installed in the through opening (103) via a retractable assembly; A sampling mechanism is installed in the sampling platform (1), and stool is dipped and collected by the sampling mechanism; A cleaning mechanism is installed in the sampling barrel (101) and is used to clean feces splashed onto the inner wall of the sampling barrel (101); Wherein, the supporting film (3) is provided with defecation grooves (301) at equal intervals along the length direction, and the distance between two defecation grooves (301) is greater than the length of a single defecation groove (301).

2. The intestinal flora sampling device based on neonatal sepsis research according to claim 1, characterized in that: The retracting and unreeling assembly comprises an unreeling roller (4) and a reeling roller (402) rotatably mounted in the sampling platform (1) and located at both ends of the sampling platform (1); A plurality of limiting rollers (401) are rotatably mounted in the sampling platform (1); A No. 1 motor (403) is fixed inside the sampling platform (1), and the No. 1 motor (403) is connected to the winding roller (402) via a belt; A flattening piece is installed in the sampling platform (1).

3. The intestinal flora sampling device based on neonatal sepsis research according to claim 2, characterized in that: The flattening member comprises two sets of rolling members rotatably mounted on both sides of the supporting film (3); The rolling element comprises two tension rollers (5), and the two tension rollers (5) are respectively located at the top and bottom of the carrier film (3); One side of each of the two tensioning rollers (5) is rotatably mounted with a number one gear set (501), the number one gear set (501) comprising two mutually meshing gears, the two gears being connected to the two number one gear sets (501) via two number one synchronous belts (502); A second gear set (503) is rotatably mounted between the two rolling elements. The two second gear sets (503) include two mutually meshing gears. The two gears are respectively connected to one of the first gear sets (501) of the two rolling elements via a second synchronous belt (504). A second motor (505) is also fixed to one side of the first gear set (501), and the second motor (505) is connected to one of the first gear sets (501) via a belt.

4. The intestinal flora sampling device based on neonatal sepsis research according to claim 3, characterized in that: The sampling mechanism comprises three supporting members (202) fixed in the sampling platform (1), a carrying ring (2) is rotatably mounted between the three supporting members (202), and the carrying ring (2) passes through the sampling barrel (101) and is sealingly and slidingly connected thereto; The bottom circumference of the carrying ring (2) is provided with a plurality of assembly grooves (203) at equal intervals, and a dipping assembly is installed in each of the plurality of assembly grooves (203). A sealing tube assembly that cooperates with the dipping assembly is also installed in the sampling station (1); A transmission wheel (201) is rotatably mounted on each of the two supporting members (202), the transmission wheel (201) and the carrying ring (2) are in rolling engagement, and a No. 1 motor (205) is fixed on each of the two supporting members (202), the output shaft of the No. 1 motor (205) being coaxially fixed with the transmission wheel (201).

5. The intestinal flora sampling device based on neonatal sepsis research according to claim 4, characterized in that: The dipping assembly comprises a No. 1 electric push rod (209) fixed in the assembly groove (203); a mounting portion (2010) is coaxially fixed to the movable rod end of the No. 1 electric push rod (209); a plurality of electromagnetic sliders (2011) for clamping are fixed in the mounting portion (2010); and a sampling piece (2012) is detachably mounted in the electromagnetic slider (2011).

6. The intestinal flora sampling device based on neonatal sepsis research according to claim 5, characterized in that: The tube sealing assembly includes a delivery member (204) fixed in the sampling platform (1), a tube supply port is provided on the top of the delivery member (204), and the tube supply port cooperates with the assembly groove (203), and an electric push rod is also fixed on the bottom of the delivery member (204), the electric push rod is located directly below the tube supply port, and the movable rod of the electric push rod passes through the bottom of the delivery member (204); A screw rod (206) is rotatably mounted on one side of the delivery member (204), and a threaded sleeve is sleeved on the screw rod (206) and is threadedly matched with the screw rod. The threaded sleeve is fixed to a push plate slidably mounted in the delivery member (204). A second motor (208) is also fixed on the assembly groove (203), and the output shaft of the second motor (208) is coaxially fixed with the screw rod (206).

7. The intestinal flora sampling device based on neonatal sepsis research according to claim 6, characterized in that: The sealing tube assembly further comprises a protective shell (2013); a top hole (2014) is provided on the top of the assembly groove (203); and a diameter of the top hole (2014) is larger than that of the sampling piece (2012); A sealing cover (2015) that matches the top hole (2014) is fixed on the rod body of the sampling piece (2012).

8. The intestinal flora sampling device based on neonatal sepsis research according to claim 1, characterized in that: The cleaning mechanism includes a plurality of through ports (103) fixed in the sampling barrel (101); A blocking cover (105) for blocking the sampling barrel (101) is also slidably mounted on the sampling platform (1), and an integrated nozzle (102) is provided at the bottom of the blocking cover (105). Both the integrated nozzle (102) and the blocking cover (105) are connected to a water supply device.

9. The intestinal flora sampling device based on neonatal sepsis research according to claim 3, characterized in that: A pressure strip (6) is slidably mounted on both sides of the sampling barrel (101), and a second electric push rod (601) is fixed on both sides of the two sampling barrels (101), and the movable rod of the second electric push rod (601) is fixed to the pressure strip (6).

10. A method for sampling intestinal flora based on neonatal sepsis research, using the intestinal flora sampling device based on neonatal sepsis research according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: The infant lies on the sampling table (1) with its buttocks positioned in the sampling bucket (101), so that feces of the infant falls into the sampling bucket (101) during defecation; Step 2: When the stool falls into the sampling bucket (101), it is received by the supporting film (3), so that the stool falls onto the supporting film (3); Step three, sampling the stool on the supporting film (3) by means of a sampling mechanism, and after the stool sampling is completed, cleaning the inside of the sampling barrel (101) by means of a cleaning mechanism.

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