Real-time fluorescent quantitative PCR (Polymerase Chain Reaction) equipment

Abstract

The invention discloses real-time fluorescent quantitative PCR equipment, and relates to the technical field of gene detection, the real-time fluorescent quantitative PCR equipment comprises a PCR equipment assembly, the PCR equipment assembly comprises a PCR main body, a first groove is formed in the PCR main body, a second groove is formed in the first groove, a fan is fixed in the second groove, and an inclined strip is fixed at the top in the second groove; and the bearing assembly is arranged in the second groove, and comprises a placing piece positioned in the second groove and a fixing frame positioned on the outer side of the PCR main body. The device has the beneficial effects that through the arrangement of the bearing assembly, when the placing piece and the driving piece are pushed in, the perforated plate and the positioning frame can be automatically settled and locked, the overlapping area of the first air hole and the second air hole is reduced, uniform heating of the side of the positioning frame is facilitated, when the placing piece and the driving piece are moved out, the perforated plate and the positioning frame are automatically ejected and locked, and the positioning efficiency is improved. The overlapping area of the first air holes and the second air holes is increased, and waste heat dissipation is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of gene detection, in particular to a real-time fluorescence quantitative PCR device. Background Art

[0002] Real-time fluorescence quantitative PCR equipment is a method that uses fluorescent chemicals to measure the total amount of products after each polymerase chain reaction (PCR) cycle in a DNA amplification reaction. It can be divided into push-pull type and flip-top type according to the operation method.

[0003] In the existing push-pull PCR equipment, test tubes are usually placed or removed after the mechanism slides out, and manual insertion is required to place or remove the test tubes. This is not only inconvenient to operate, but also easily causes the test tubes to shake or even slip. At the same time, the simple placement design makes it difficult to dissipate heat efficiently when removing, and the temperature uniformity of the test tubes on the sides is generally poor when pushing in, which cannot meet actual usage needs. Summary of the Invention

[0004] In view of the above problems existing in the existing real-time fluorescence quantitative PCR equipment, the present invention is proposed.

[0005] Therefore, the problem to be solved by the present invention is that the push-pull PCR equipment in the prior art requires manual insertion to place or take out the test tubes, which is not only inconvenient to operate, but also easily causes the test tubes to shake or even slip. At the same time, the simple placement design makes it difficult to dissipate heat efficiently when removing, and the temperature uniformity of the test tubes on the sides is generally poor when pushing them in.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a real-time fluorescence quantitative PCR device, comprising: A PCR device assembly includes a PCR body, wherein the PCR body is provided with a first groove, a second groove is provided in the first groove, a fan is fixed in the second groove, and an oblique bar is fixed on the top of the second groove; and The bearing assembly is arranged in the second groove, includes a placement piece located in the second groove, includes a fixed frame located outside the PCR body, a positioning frame is slidably connected in the fixed frame, a porous plate is placed on the positioning frame, side frames are fixed on both sides of the positioning frame, a first air hole is opened on one side of the side frame, a sliding sleeve is provided on the outside of the side frame, and positioning heads are slidably connected to both ends of the sliding sleeve, a guide rail is fixed in the second groove, a sliding seat is slidably connected on the guide rail, a second air hole is opened on one side of the sliding seat, a locking piece is provided on the sliding seat, and a driving piece is provided on the fixed frame, including a sealing plate fixed on the fixed frame, a displacement frame is slidably connected in the sealing plate, a pressing piece is provided on one side of the sealing plate, and a positioning sleeve is embedded in the first groove.

[0007] As a preferred solution of the real-time fluorescence quantitative PCR device of the present invention, through slots are provided on both sides of the fixing frame, and an electric heating plate is fixed in the fixing frame.

[0008] As a preferred solution of the real-time fluorescence quantitative PCR device described in the present invention, the locking member includes a slide bar fixed on the sealing plate, a limit plate is provided in the slide seat, a positioning groove is provided on the limit plate, a first oblique groove is provided at the bottom of the positioning groove, and a second spring is fixed to the bottom of the limit plate.

[0009] As a preferred solution of the real-time fluorescence quantitative PCR device described in the present invention, a displacement groove is opened on one side of the slide, the slide bar and the limit plate are both slidably connected in the displacement groove, and the other end of the second spring is fixed in the displacement groove.

[0010] As a preferred solution of the real-time fluorescence quantitative PCR device of the present invention, the outer ring of the positioning head is provided with a limiting groove, which cooperates with a limiting plate.

[0011] As a preferred solution of the real-time fluorescence quantitative PCR device described in the present invention, a support member is provided at the bottom of the positioning frame, including a support rod slidably connected to the fixed frame, a first spring is sleeved on the support rod, and an auxiliary groove is opened at the top of the fixed frame.

[0012] As a preferred solution of the real-time fluorescence quantitative PCR device of the present invention, one end of the first spring is fixed on the supporting rod, and the other end of the first spring is fixed in the auxiliary groove.

[0013] As a preferred embodiment of the real-time fluorescence quantitative PCR device of the present invention, a mounting groove is provided in the sealing plate and cooperates with the displacement rack. A protrusion is fixed at the center of one side of the displacement rack, a fourth spring is fixed on the protrusion, and the other end of the fourth spring is fixed in the mounting groove.

[0014] As a preferred solution of the real-time fluorescence quantitative PCR device described in the present invention, a receiving groove is opened in the first groove, the positioning sleeve is slidably connected to the receiving groove, a third spring is fixed on one side of the positioning sleeve, and the other end of the third spring is fixed in the receiving groove.

[0015] As a preferred solution of the real-time fluorescence quantitative PCR device described in the present invention, the buckling part includes an auxiliary frame fixed on the sealing plate, a buckle block is movably connected to the auxiliary frame, a protrusion is fixed on the buckle block, a torsion spring is fixed on the top and bottom of the buckle block, the other end of the torsion spring is fixed on the auxiliary frame, an inclined plate is provided on the buckle block, and a card slot is provided in the first groove and cooperates with the buckle block.

[0016] The beneficial effects of the present invention are as follows: through the arrangement of the bearing assembly, when the placement member and the driving member are pushed in, the porous plate and the positioning frame are automatically sunk and locked, and the overlapping area of ​​the first air hole and the second air hole is reduced, which is beneficial to the heat distribution on the sides of the positioning frame; when the placement member and the driving member are moved out, the porous plate and the positioning frame are automatically ejected and locked, and the overlapping area of ​​the first air hole and the second air hole is increased, which is beneficial to the dissipation of residual heat and is more in line with actual use requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0018] Figure 1 This is a structural diagram of the real-time fluorescence quantitative PCR equipment.

[0019] Figure 2 A front view of the PCR equipment components of a real-time fluorescence quantitative PCR device.

[0020] Figure 3 This is a structural diagram of the supporting components of the real-time fluorescence quantitative PCR device.

[0021] Figure 4 A diagram showing the placement of components for real-time fluorescence quantitative PCR equipment.

[0022] Figure 5 This is another perspective view of the real-time fluorescence quantitative PCR device when the sliding sleeve and the sliding base are separated.

[0023] Figure 6 For real-time fluorescence quantitative PCR equipment Figure 5 Enlarged view of point A in the middle.

[0024] Figure 7 This is a diagram showing the separation of the driving components of the real-time fluorescence quantitative PCR device.

[0025] Figure 8 This is a structural diagram of the buckle of the real-time fluorescence quantitative PCR device.

[0026] Figure 9 It is an oblique side view of the real-time fluorescence quantitative PCR device.

[0027] In the figure: 1. PCR equipment assembly; 11. PCR body; 11-1. First groove; 11-2. Second groove; 11-3. Card slot; 11-4. Storage slot; 12. Fan; 13. Oblique bar; 2. Carrying assembly; 21. Placement member; 21-1. Fixing frame; 21-11. Through groove; 21-2. Hot plate; 21-3. Positioning frame; 21-4. Perforated plate; 21-5. Side frame; 21-51. First air hole; 21-6. Sliding sleeve; 21-61. Positioning head; 21-611. Limiting groove; 21-7. Support member; 21-71. Support rod; 21-72. First spring; 21-73. Auxiliary groove; 21-8. Guide rail; 21-9, slide seat; 21-91, second air hole; 21-92, displacement groove; 21-10, locking member; 21-101, slide bar; 21-102, limit plate; 21-103, positioning groove; 21-104, first inclined groove; 21-105, second spring; 22, driving member; 22-1, sealing plate; 22-11, mounting groove; 22-2, displacement frame; 22-21, convex seat; 22-22, fourth spring; 22-3, pressing member; 22-31, auxiliary frame; 22-32, buckle block; 22-33, convex block; 22-34, torsion spring; 22-35, inclined plate; 22-4, positioning sleeve; 22-41, third spring. DETAILED DESCRIPTION

[0028] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0029] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments. Example 1

[0031] Reference Figures 1 to 7, which is the first embodiment of the present invention, provides a real-time fluorescence quantitative PCR device, which includes a PCR device component 1 and a carrier component 2. Through the setting of the carrier component 2, it can complete automatic sedimentation and locking during the push-in operation, and the ventilation area is reduced, which is conducive to heat distribution on the sides. During the removal operation, it can complete automatic ejection and locking, and the ventilation overlap area is increased, which is conducive to the dissipation of residual heat.

[0032] Specifically, the PCR device assembly 1 includes a PCR body 11, which is provided with a first groove 11-1, a second groove 11-2 in the first groove 11-1, a fan 12 fixed in the second groove 11-2, and an oblique bar 13 fixed at the top of the second groove 11-2.

[0033] There is a ventilation space between the fan 12 and the second groove 11-2. The fan 12 is fixed in the second groove 11-2 by a bracket screw. When the fan 12 is working, it promotes the flow of air to evenly distribute the heat generated by the heating module such as the metal base or heating block of the thermal cycler to all reaction holes. Similarly, in the annealing and cooling stage, the fan cooperates with the refrigeration module such as the semiconductor cooler to accelerate heat exchange, shorten the temperature conversion time, and improve the experimental efficiency. The working principles of this part are all existing technologies, which can be clearly understood by those skilled in the art and will not be elaborated here.

[0034] Specifically, the supporting component 2 is arranged in the second groove 11-2, including a placement piece 21 located in the second groove 11-2, including a fixing frame 21-1 located on the outside of the PCR body 11, a positioning frame 21-3 is slidably connected in the fixing frame 21-1, a porous plate 21-4 is placed on the positioning frame 21-3, side frames 21-5 are fixed on both sides of the positioning frame 21-3, a first air hole 21-51 is opened on one side of the side frame 21-5, a sliding sleeve 21-6 is provided on the outside of the side frame 21-5, and positioning heads 21-61 are slidably connected at both ends of the sliding sleeve 21-6.

[0035] A guide rail 21-8 is fixed in the second groove 11-2, and a slide 21-9 is slidably connected to the guide rail 21-8. A second air hole 21-91 is opened on one side of the slide 21-9, and a locking member 21-10 is provided on the slide 21-9. A driving member 22 is provided on the fixed frame 21-1, including a sealing plate 22-1 fixed on the fixed frame 21-1, a displacement frame 22-2 is slidably connected in the sealing plate 22-1, and a buckling member 22-3 is provided on one side of the sealing plate 22-1. A positioning sleeve 22-4 is embedded in the first groove 11-1.

[0036] The fixed frame 21 - 1 is fixedly connected to the slide 21 - 9 . When the slide 21 - 9 moves in a push or pull manner, the fixed frame 21 - 1 will also move synchronously.

[0037] There are two positioning heads 21-61, and the centers of the two positioning heads 21-61 are rotatably connected to positioning rods. One positioning head 21-61 is installed on the first air hole 21-51 through the positioning rod, and the other positioning head 21-61 is installed in the second groove 11-2 through the positioning rod.

[0038] By setting the locking piece 21-10, the positioning head 21-61 can be locked after the push-pull operation is completed, and the angle of the positioning head 21-61 can be limited, thereby completing the high or low position locking of the positioning frame 21-3 and the side frame 21-5, and the overall operational stability is better. Example 2

[0039] Reference Figures 2 to 9 , which is the second embodiment of the present invention, and this embodiment is based on the previous embodiment.

[0040] Specifically, through slots 21 - 11 are provided on both sides of the fixing frame 21 - 1 , and the electric heating plate 21 - 2 is fixed inside the fixing frame 21 - 1 .

[0041] The electric heating plate 21-2 is located at the bottom of the positioning frame 21-3. The positioning frame 21-3 and the porous plate 21-4 are both made of 6061 aluminum alloy, which has a thermal conductivity of approximately 167W / (m·K), combining good thermal conductivity and mechanical strength. The surface of the positioning frame 21-3 is anodized to enhance corrosion resistance and ensure long-term stable use in the PCR experimental environment.

[0042] When the placement member 21 and the driving member 22 are pushed in, the positioning frame 21 - 3 will automatically sink and contact the top of the electric heating plate 21 - 2 to achieve subsequent heat exchange and heating.

[0043] The locking member 21-10 includes a slide bar 21-101 fixed on the sealing plate 22-1, a limiting plate 21-102 is arranged in the slide seat 21-9, a positioning groove 21-103 is opened on the limiting plate 21-102, a first inclined groove 21-104 is opened at the bottom of the positioning groove 21-103, and a second spring 21-105 is fixed to the bottom of the limiting plate 21-102.

[0044] Through the setting of the first inclined groove 21-104, when the slide bar 21-101 moves, it can contact the first inclined groove 21-104 and press the limit plate 21-102 to move downward.

[0045] By setting the second spring 21-105, the limit plate 21-102 can be elastically supported, and the limit plate 21-102 can be prevented from moving downward at will in the absence of external force.

[0046] A displacement groove 21-92 is provided on one side of the slide 21-9, the slide bar 21-101 and the limit plate 21-102 are both slidably connected in the displacement groove 21-92, and the other end of the second spring 21-105 is fixed in the displacement groove 21-92.

[0047] By setting the displacement groove 21-92, the movement space requirements of the slider 21-101 and the limit plate 21-102 can be met.

[0048] The outer ring of the positioning head 21-61 is provided with a limiting groove 21-611, and cooperates with the limiting plate 21-102.

[0049] The setting of the limiting plate 21-102 and the limiting groove 21-611 can play a role in positioning and preventing disengagement. When the limiting groove 21-611 is inserted into the limiting plate 21-102, the positioning head 21-61 is prevented from rotating and changing angles at will.

[0050] A support member 21-7 is provided at the bottom of the positioning frame 21-3, including a support rod 21-71 slidably connected to the fixed frame 21-1, a first spring 21-72 is sleeved on the support rod 21-71, and an auxiliary groove 21-73 is opened at the top of the fixed frame 21-1.

[0051] One end of the first spring 21-72 is fixed on the supporting rod 21-71, and the other end of the first spring 21-72 is fixed in the auxiliary groove 21-73.

[0052] The first spring 21-72 can play a role of elastic support and prevent the support rod 21-71 from moving downward at will in the absence of external force.

[0053] By setting the auxiliary groove 21-73, the displacement of the support rod 21-71 can be positioned and guided to ensure the stability of the movement of the support rod 21-71.

[0054] A mounting groove 22-11 is provided in the sealing plate 22-1 and cooperates with the displacement frame 22-2. A convex seat 22-21 is fixed at the center of one side of the displacement frame 22-2. A fourth spring 22-22 is fixed on the convex seat 22-21. The other end of the fourth spring 22-22 is fixed in the mounting groove 22-11.

[0055] The connection between the displacement frame 22-2 and the installation groove 22-11, and the connection between the protrusion 22-21 and the installation groove 22-11 are in sliding contact. Through the setting of the installation groove 22-11, the displacement space requirements of the displacement frame 22-2 can be met. Through the setting of the protrusion 22-21, it is convenient for the operator to press, thereby driving the displacement frame 22-2 to move.

[0056] By providing the fourth spring 22 - 22 , the convex seat 22 - 21 can be elastically supported, and the convex seat 22 - 21 and the displacement frame 22 - 2 can be prevented from being randomly displaced in the absence of external force.

[0057] A receiving groove 11-4 is provided in the first groove 11-1, and a positioning sleeve 22-4 is slidably connected in the receiving groove 11-4. A third spring 22-41 is fixed to one side of the positioning sleeve 22-4, and the other end of the third spring 22-41 is fixed in the receiving groove 11-4.

[0058] By setting the positioning sleeve 22-4 and the third spring 22-41, when the sealing plate 22-1 is pushed in for installation, the positioning sleeve 22-4 can be pressed against and the third spring 22-41 can be compressed, thereby providing elastic support force for the sealing plate 22-1 during the removal operation, thereby driving the sealing plate 22-1 to move a certain distance, which is beneficial for the operator to pull out the sealing plate 22-1 by hand.

[0059] The buckling member 22-3 includes an auxiliary frame 22-31 fixed on the sealing plate 22-1, a buckle block 22-32 is movably connected to the auxiliary frame 22-31, a protrusion 22-33 is fixed to the buckle block 22-32, a torsion spring 22-34 is fixed to the top and bottom of the buckle block 22-32, the other end of the torsion spring 22-34 is fixed to the auxiliary frame 22-31, an inclined plate 22-35 is provided on the buckle block 22-32, and a card slot 11-3 is provided in the first groove 11-1, which cooperates with the buckle block 22-32.

[0060] The side of the protrusion 22-33 away from the buckle block 22-32 contacts the displacement frame 22-2, and the side of the slide bar 21-101 away from the limiting plate 21-102 contacts the displacement frame 22-2.

[0061] By setting the torsion spring 22-34, a torsion force can be provided for the buckle block 22-32, thereby preventing the buckle block 22-32 from rotating and opening at will without the action of external force.

[0062] By setting the buckle block 22-32 and the card slot 11-3, when the buckle block 22-32 is inserted into the card slot 11-3, it can play the role of a limit lock, thereby ensuring the installation stability of the sealing plate 22-1.

[0063] Through the setting of the torsion spring 22-34, when the sealing plate 22-1 is stably displaced, the inclined plate 22-35 can contact the PCR body 11, so that the buckle block 22-32 automatically rotates open, and the torsion spring 22-34 is tightened. When the buckle block 22-32 is aligned with the slot 11-3, under the action of the torsion spring 22-34, the buckle block 22-32 will automatically be stuck in the slot 11-3.

[0064] The oblique strip 13 is viewed from the side as shown in the accompanying drawings of the specification. Figure 9As shown, it includes an oblique rod, a guide rod and a flat rod. The oblique rod is fixed to the top of the second groove 11-2, the other end of the oblique rod is fixed to the guide rod, and the other end of the guide rod is fixed to the flat rod. It is supported by the first spring 21-72 and the support rod 21-71. The positioning frame 21-3 is a protruding setting. When the positioning frame 21-3 moves backward, it will contact the guide rod on the oblique bar 13 to squeeze the positioning frame 21-3 to automatically move downward until the top of the positioning frame 21-3 is flush with the bottom of the flat rod. At this time, the top of the positioning frame 21-3 is also flush with the top of the fixed frame 21-1.

[0065] When in use, the carrying component 2 is in a pulled-out and exposed state, and the positioning frame 21-3 is protruding compared to the fixed frame 21-1. At this time, it is only necessary to move the porous plate 21-4 carrying the sample tube down, place it in the positioning frame 21-3, press the protrusion 22-21, and then push the sealing plate 22-1 into the first groove 11-1. The overlapping area of ​​the first air hole 21-51 and the second air hole 21-91 is reduced, and the buckle block 22-32 is locked in the card slot 11-3 to complete the locking.

[0066] When the protrusion 22-21 is pressed, the fourth spring 22-22 is compressed, the displacement frame 22-2 is displaced and presses the slide bar 21-101. With the assistance of the first inclined groove 21-104, the limit plate 21-102 can be pressed to move downward stably and disengage from the limit groove 21-611, and the second spring 21-105 is compressed.

[0067] When the above-mentioned limiting plate 21-102 is out of the limiting groove 21-611, the driving member 22 and the placing member 21 are pushed into the second groove 11-2, and are positioned and guided by the guide rail 21-8 and the slide 21-9, thereby improving the movement stability of the fixed frame 21-1 and the sealing plate 22-1 and changing the inclination angle of the positioning head 21-61.

[0068] As the sealing plate 22-1 moves, the upper inclined plate 22-35 of the buckle block 22-32 contacts the PCR body 11, squeezing the buckle block 22-32 to rotate open, and the torsion spring 22-34 tightens until the buckle block 22-32 is stuck in the slot 11-3, ensuring the position stability of the sealing plate 22-1 and the fixed frame 21-1.

[0069] As the sealing plate 22-1 moves, the positioning frame 21-3 contacts the oblique bar 13 and is squeezed, automatically sinking into the fixed frame 21-1, and the support rod 21-71 synchronously moves downward in the auxiliary groove 21-73, and the first spring 21-72 is compressed until the bottom of the positioning frame 21-3 contacts the top of the electric heating plate 21-2, and the fixed frame 21-1 and the sealing plate 22-1 are both locked and installed. At this time, the limit plate 21-102 is aligned with the new limit groove 21-611.

[0070] Release and stop pressing on the protrusion 22-21. Under the elastic support of the second spring 21-105, the limit plate 21-102 will move up and reset into the new limit groove 21-611, completing the locking of the positioning head 21-61 and ensuring the installation stability of the positioning frame 21-3 and the side frame 21-5.

[0071] The side frame 21-5 moves downward synchronously with the positioning frame 21-3, and the overlapping area of ​​the first air hole 21-51 and the second air hole 21-91 thereof is reduced. In this state, when the PCR equipment component 1 is working to provide heat, the fan 12 works synchronously to suppress violent air convection and slow down the loss of internal heat to the external environment. In conjunction with the through slot 21-11, the purpose of evenly dissipating heat on the sides of the positioning frame 21-3 is achieved, especially when the power of the electric heating plate 21-2 is limited or a constant temperature needs to be maintained, which can improve thermal efficiency and reduce energy consumption.

[0072] Similarly, when the pulling-out operation is required, press the protrusion 22-21 to disengage the limit plate 21-102 from the limit groove 21-611 and the buckle block 22-32 from the card slot 11-3, and then pull out the sealing plate 22-1 and the fixed frame 21-1 until the positioning frame 21-3 automatically moves up and protrudes, and the limit plate 21-102 is aligned with the new limit groove 21-611, release the protrusion 22-21, and the limit plate 21-102 is snapped into the limit groove 21-611 to ensure the height of the positioning frame 21-3 and the side frame 21-5, which is conducive to removing or re-placing the positioning frame 21-3.

[0073] In this state, the overlapping area of ​​the first air hole 21-51 and the second air hole 21-91 increases, forming a large hole. The large hole increases the contact area with the outside air, which is equivalent to the "heat sink fin" effect, accelerating heat dissipation through the dual effects of convection and radiation. Example 3

[0074] Reference Figures 3 to 9 , which is the third embodiment of the present invention, and is based on the first two embodiments.

[0075] Specifically, a finger groove is provided on the sealing plate 22-1 for the operator to insert his fingers, making it convenient to pull the sealing plate 22-1, while leaving the thumb free to press the protrusion 22-21. It conforms to the ergonomic design and is more comfortable to use.

[0076] In actual application, the overlapping area of ​​the first pores 21-51 and the second pores 21-91 is reduced to form small holes, so that heat can diffuse slowly near the reaction area, avoiding local temperature fluctuations caused by strong convection.

[0077] In actual application, the overlapping area of ​​the first air hole 21-51 and the second air hole 21-91 increases to form a large hole, and the fan 12 can also be controlled to work alone to form a forced air duct, accelerate air flow, and significantly improve the heat dissipation rate.

[0078] The fan 12 cooperates with the electric heating plate 21-2 and the built-in temperature control system of the PCR equipment component 1 to achieve a heating temperature range of 40-95°C for the equipment, a temperature control accuracy of ±0.1°C, and a heating and cooling rate of ≥5°C / s; under a constant temperature of 95°C, the temperature deviation of each well of the multi-well plate 21-4 is ≤±0.3°C, ensuring the accuracy and consistency of the temperature conditions in the PCR experiment. The working principles of this part are all existing technologies, which can be clearly understood by those skilled in the art and will not be elaborated here. Example 4

[0079] Reference Figures 1 to 8 , which is the fourth embodiment of the present invention, and is based on the first three embodiments.

[0080] Specifically, the fluorescence detection module of the PCR equipment component 1 includes an excitation light source, a fluorescence detector, a filter set and an optical path system. The excitation light source uses a 470nm high-power LED, which is focused by a lens and vertically irradiated to the samples in the multi-well plates 1-4; the fluorescence detector is a high-sensitivity PMT, which receives the 520nm fluorescence signal generated by the sample through the emission filter.

[0081] During the PCR cycle, the temperature control component precisely controls the temperature at the set value. When entering the annealing and extension stages, the fluorescence detection module automatically starts signal acquisition. The collected fluorescence signal is amplified and transmitted to the control system after analog-to-digital conversion. The Ct value is calculated by the built-in analysis software to achieve quantitative analysis of the sample nucleic acid. At the same time, the control system compensates and corrects the fluorescence signal based on real-time temperature data to ensure the accuracy of the test results. The working principles of this part are all existing technologies and are clearly understood by those skilled in the art, so they will not be elaborated here.

[0082] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A real-time fluorescence quantitative PCR device, characterized in that: include, A PCR device assembly (1) comprises a PCR body (11), wherein a first groove (11-1) is provided on the PCR body (11), a second groove (11-2) is provided in the first groove (11-1), a fan (12) is fixed in the second groove (11-2), and an oblique strip (13) is fixed at the top of the second groove (11-2); and The bearing assembly (2) is arranged in the second groove (11-2), including a placement member (21) located in the second groove (11-2), and including a fixing frame (21-1) located outside the PCR body (11), wherein a positioning frame (21-3) is slidably connected in the fixing frame (21-1), and side frames (21-5) are fixed on both sides of the positioning frame (21-3), and a first air hole (21-51) is opened on one side of the side frame (21-5), and a sliding sleeve (21-6) is provided on the outside of the side frame (21-5), and both ends of the sliding sleeve (21-6) are slidably connected. A positioning head (21-61) is connected, a slide seat (21-9) is provided in the second groove (11-2), a second air hole (21-91) is provided on one side of the slide seat (21-9), a locking member (21-10) is provided on the slide seat (21-9), and a driving member (22) is provided on the fixed frame (21-1), comprising a sealing plate (22-1) fixed on the fixed frame (21-1), a displacement frame (22-2) is slidably connected in the sealing plate (22-1), and a pressing member (22-3) is provided on one side of the sealing plate (22-1).

2. The real-time fluorescence quantitative PCR device according to claim 1, wherein: Through grooves (21-11) are provided on both sides of the fixing frame (21-1), an electric heating plate (21-2) is fixed in the fixing frame (21-1), a porous plate (21-4) is placed on the positioning frame (21-3), a guide rail (21-8) is fixed in the second groove (11-2), and the slide seat (21-9) is slidably connected to the guide rail (21-8).

3. The real-time fluorescence quantitative PCR device according to claim 1, wherein: The locking member (21-10) comprises a slide bar (21-101) fixed on a sealing plate (22-1); a limiting plate (21-102) is provided in the sliding seat (21-9); a positioning groove (21-103) is provided on the limiting plate (21-102); a first inclined groove (21-104) is provided at the bottom of the positioning groove (21-103); and a second spring (21-105) is fixed to the bottom of the limiting plate (21-102).

4. The real-time fluorescence quantitative PCR device according to claim 3, wherein: A displacement groove (21-92) is provided on one side of the slide seat (21-9), the slide bar (21-101) and the limit plate (21-102) are both slidably connected in the displacement groove (21-92), and the other end of the second spring (21-105) is fixed in the displacement groove (21-92).

5. The real-time fluorescence quantitative PCR device according to claim 3, wherein: The outer ring of the positioning head (21-61) is provided with a limiting groove (21-611) and cooperates with the limiting plate (21-102).

6. The real-time fluorescence quantitative PCR device according to claim 1, wherein: A support member (21-7) is provided at the bottom of the positioning frame (21-3), comprising a support rod (21-71) slidably connected to the fixed frame (21-1), a first spring (21-72) being sleeved on the support rod (21-71), and an auxiliary groove (21-73) is provided at the top of the fixed frame (21-1).

7. The real-time fluorescence quantitative PCR device according to claim 6, wherein: One end of the first spring (21-72) is fixed on the supporting rod (21-71), and the other end of the first spring (21-72) is fixed in the auxiliary groove (21-73).

8. The real-time fluorescence quantitative PCR device according to claim 1, wherein: A mounting groove (22-11) is provided in the sealing plate (22-1) and cooperates with the displacement frame (22-2). A convex seat (22-21) is fixed at the center of one side of the displacement frame (22-2). A fourth spring (22-22) is fixed on the convex seat (22-21). The other end of the fourth spring (22-22) is fixed in the mounting groove (22-11).

9. The real-time fluorescence quantitative PCR device according to claim 1, wherein: A positioning sleeve (22-4) is embedded in the first groove (11-1), a receiving groove (11-4) is provided in the first groove (11-1), the positioning sleeve (22-4) is slidably connected to the receiving groove (11-4), a third spring (22-41) is fixed to one side of the positioning sleeve (22-4), and the other end of the third spring (22-41) is fixed in the receiving groove (11-4).

10. The real-time fluorescence quantitative PCR device according to claim 1, wherein: The buckling member (22-3) comprises an auxiliary frame (22-31) fixed on the sealing plate (22-1); a buckle block (22-32) is movably connected to the auxiliary frame (22-31); a protrusion (22-33) is fixed to the buckle block (22-32); a torsion spring (22-34) is fixed to the top and bottom of the buckle block (22-32); the other end of the torsion spring (22-34) is fixed to the auxiliary frame (22-31); an inclined plate (22-35) is provided on the buckle block (22-32); and a clamping slot (11-3) is provided in the first groove (11-1) and cooperates with the buckle block (22-32).

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