Stem cell culture device

Abstract

The invention provides a stem cell culture device, and relates to the field of stem cell culture, the stem cell culture device comprises a bottom plate and a controller, the upper end of the bottom plate is provided with a base, the upper end of the base is provided with a sealing bin, the lower end of the bottom plate is provided with supporting legs, the upper ends of inner cavities of the supporting legs are provided with first hydraulic rods, and the lower ends of the first hydraulic rods are located in the supporting legs; the telescopic end of the first hydraulic rod is fixedly connected with the bottom end of the bottom plate, the bottom end of the first hydraulic rod is fixedly connected with an air spring, dampers are arranged on the two sides of the air spring, a containing table is arranged in the sealing bin, a containing plate is rotationally connected into the containing table, and third hydraulic rods are arranged on the two sides of the bottom end of an inner cavity of the containing plate. The stem cell culture device has the advantages that by arranging the damper and the air spring, external vibration can be effectively isolated, the high stability of the whole device during operation is ensured, by arranging the third hydraulic rods, the two third hydraulic rods are contracted according to a rule, an irregular fluid oscillation mode is simulated, and the requirements of different stem cell culture for diversified physical stimulation are met.

Description

Technical Field

[0001] The present invention relates to the field of stem cell culture, and in particular to a stem cell culture device. Background Art

[0002] Stem cells are a special type of cell with the ability to self-renew and multidirectional differentiation potential. Depending on their origin and differentiation ability, they can be mainly divided into embryonic stem cells, adult stem cells (such as bone marrow mesenchymal stem cells, neural stem cells, etc.) and induced pluripotent stem cells. In the fields of modern medicine and biology, stem cells have shown great application potential. They can differentiate into various specific types of cells, such as neurons for the treatment of neurological diseases, cardiomyocytes to repair damaged heart tissue, and pancreatic islet cells to improve insulin secretion in diabetic patients. In addition, stem cells play a key role in many aspects such as drug screening, disease model construction and regenerative medicine, bringing new hope for overcoming some difficult diseases and improving human health.

[0003] Publication number CN 118530835 A discloses a stem cell culture device, belonging to the field of stem cell culture. The device comprises a base and a sealed chamber fixed to its top, further comprising: a supporting platform rotatably connected to the sealed chamber, the base being provided with a shaking portion for driving the supporting platform to rotate, wherein a culture dish is placed on the supporting platform, and clamping portions are provided on both sides of the supporting platform for securing the culture dish. The present invention drives the supporting platform to rotate periodically, causing the culture dish fixed to the supporting platform to undergo a rotational shaking effect, allowing the stem cells in the culture dish to be evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of the stem cells and improving the culture effect. The heat generated by driving the supporting platform to rotate is transferred to a heating tank, adaptively heating the culture dish, providing a suitable temperature environment, improving the culture effect of the stem cells, and utilizing the heat generated during operation, which is more energy-efficient and environmentally friendly. Conventional methods can only rotate and shake the culture dish, which has a single stimulation method and generates vibration during use, which can affect the stem cell culture. Summary of the Invention

[0004] The purpose of the present invention is to provide a stem cell culture device that solves the technical problem that the culture dish can only be rotated and shaken, the stimulation method is single, and vibration is generated during the use of the device, which affects the stem cell culture.

[0005] In order to achieve the above-mentioned object of the invention, the technical solution adopted by the present invention is:

[0006] A stem cell culture device comprises a base plate and a controller, wherein the upper end of the base plate is provided with a base, the upper end of the base is provided with a sealed chamber, the lower end of the base plate is provided with a support leg, the upper end of the inner cavity of the support leg is provided with a first hydraulic rod, the lower end of the first hydraulic rod is located in the support leg, the telescopic end of the first hydraulic rod is fixedly connected to the bottom end of the base plate, the bottom end of the first hydraulic rod is fixedly connected to an air spring, dampers are provided on both sides of the air spring, the lower ends of the two dampers are fixedly connected to the bottom end of the inner cavity of the support leg, the upper ends of the two dampers are fixedly connected to the first hydraulic rod The bottom end is fixedly connected, and a placing table is provided in the sealed chamber, and a placing plate is rotatably connected in the placing table. Support plates are fixedly connected on both sides of the upper ends of the two placing plates, and second hydraulic rods are fixedly provided at the inner ends of the two support plates. The telescopic ends of the two second hydraulic rods are fixedly connected with splints, and pressure sensors are provided at the inner ends of the two splints. A heating groove is provided between the two splints, and the heating groove is opened on the placing plate. Third hydraulic rods are provided on both sides of the bottom end of the inner cavity of the placing plate, and the telescopic ends of the two third hydraulic rods are fixedly connected to the bottom end of the placing plate.

[0007] As an improvement, the sealed chamber adopts a double-layer sealing structure, including an inner layer and an outer layer. The inner layer is made of high-purity polytetrafluoroethylene material, and the outer layer is made of hard and transparent polycarbonate material. A vacuum insulation layer is provided between the two layers. The polytetrafluoroethylene material has good chemical stability and extremely low gas permeability. The polycarbonate material improves the overall visibility while ensuring good sealing, making it convenient for scientific researchers to observe the culture status of stem cells without opening the chamber body. The vacuum insulation layer reduces heat exchange, which is conducive to maintaining a stable temperature in the chamber.

[0008] As an improvement, a first electromagnetic block is provided at the lower end of the sealing chamber, a sealing gasket is provided around the first electromagnetic block, a groove is provided at the upper end of the base, and a second electromagnetic block is provided at the bottom end of the groove. The first electromagnetic block cooperates with the second electromagnetic block, which has better sealing and convenient operation.

[0009] As an improvement, the bottom end of the placing table is fixedly connected to a rotating rod, which extends into the base, and a driven gear is fixedly sleeved on the surface of the rotating rod. A forward and reverse motor is provided on one side of the rotating rod, and the forward and reverse motor is installed at the bottom end of the inner cavity of the base. The output end of the forward and reverse motor is connected to a driving gear, and the driving gear and the driven gear are meshed with each other. An annular slider is fixed to the bottom end of the placing table, and an annular groove is provided at the upper end of the base. The annular slider slides in cooperation with the annular groove, driving the culture dish to rotate and shake, making the device more stable.

[0010] As an improvement, sound insulation cotton is provided in the base to reduce noise.

[0011] As an improvement, a heater is provided at the lower end of the heating tank, and a temperature sensor is provided in the heating tank to heat the culture dish and sense the temperature of the culture dish.

[0012] The beneficial effects of the present invention are: by setting up a damper and an air spring, external vibrations can be effectively isolated, ensuring the high stability of the entire device during operation, and reducing the interference of external vibrations on stem cell culture. By setting up a third hydraulic rod, the two third hydraulic rods contract regularly to simulate irregular fluid oscillation patterns, meeting the needs of different stem cell cultures for diversified physical stimulation. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a front view of a stem cell culture device according to the present invention;

[0014] Figure 2 This is a schematic diagram of the interior of a stem cell culture device of the present invention;

[0015] Figure 3 This is a top view of a placement platform of a stem cell culture device of the present invention.

[0016] In the figure: 1. Bottom plate; 2. Base; 3. Sealing chamber; 4. Outer layer; 5. Vacuum insulation layer; 6. Inner layer; 7. Sealing gasket; 8. Groove; 9. First electromagnetic block; 10. Second electromagnetic block; 11. Sound insulation cotton; 12. First hydraulic rod; 13. Support leg; 14. Damper; 15. Air spring; 16. Forward and reverse motor; 17. Driving gear; 18. Driven gear; 19. Placement table; 20. Annular slide; 21. Annular slider; 22. Placement plate; 23. Support plate; 24. Second hydraulic rod; 25. Clamp; 26. Pressure sensor; 27. Heating tank; 28. Heater; 29. ​​Third hydraulic rod; 30. Temperature sensor; 31. Controller; 32. Turning rod. DETAILED DESCRIPTION

[0017] To make the contents of the present invention more clearly understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Identical parts are denoted by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "up," and "down" used in the following description refer to directions in the accompanying drawings, and the terms "inward" and "outward" refer to directions toward or away from the geometric center of a particular component, respectively.

[0018] like Figure 1 and Figure 3As shown, a stem cell culture device includes a base plate 1 and a controller 31. The upper end of the base plate 1 is provided with a base 2, and the base 2 is provided with sound insulation cotton 11 to reduce noise. The upper end of the base 2 is provided with a sealed chamber 3, and the lower end of the base plate 1 is provided with a supporting leg 13. The upper end of the inner cavity of the supporting leg 13 is provided with a first hydraulic rod 12, and the lower end of the first hydraulic rod 12 is located in the supporting leg 13. The telescopic end of the first hydraulic rod 12 is fixedly connected to the bottom end of the base plate 1, and the bottom end of the first hydraulic rod 12 is fixedly connected to an air spring 15. Both sides of the air spring 15 are provided with a damper 14, and the lower ends of the two dampers 14 are fixedly connected to the bottom end of the inner cavity of the supporting leg 13, and the upper ends of the two dampers 14 are fixedly connected to the bottom end of the first hydraulic rod 12. There is a placing table 19, and a placing plate 22 is rotatably connected to the placing table 19. Both sides of the upper ends of the two placing plates 22 are fixedly connected to support plates 23. The inner ends of the two support plates 23 are fixedly provided with second hydraulic rods 24, and the telescopic ends of the two second hydraulic rods 24 are fixedly connected to clamps 25. The inner ends of the two clamps 25 are provided with pressure sensors 26. A heating groove 27 is provided between the two clamps 25. The heating groove 27 is opened on the placing plate 22. Third hydraulic rods 29 are provided on both sides of the bottom end of the inner cavity of the placing plate 22. The telescopic ends of the two third hydraulic rods 29 are fixedly connected to the bottom end of the placing plate 22. A heater 28 is provided at the lower end of the heating groove 27. A temperature sensor 30 is provided in the heating groove 27 to heat the culture dish and sense the temperature of the culture dish.

[0019] The sealed chamber 3 adopts a double-layer sealing structure, including an inner layer 6 and an outer layer 4. The inner layer 6 is made of high-purity polytetrafluoroethylene, and the outer layer 4 is made of hard and transparent polycarbonate. A vacuum insulation layer 5 is provided between the two layers. The polytetrafluoroethylene material has good chemical stability and extremely low gas permeability. The polycarbonate material improves the overall visibility while ensuring good sealing, making it convenient for scientific researchers to observe the culture status of stem cells without opening the chamber. The vacuum insulation layer 5 reduces heat exchange and is conducive to maintaining a stable temperature in the chamber. A first electromagnetic block 9 is provided at the lower end of the sealed chamber 3, and a sealing gasket 7 is provided around the first electromagnetic block 9. A groove 8 is provided at the upper end of the base 2, and a second electromagnetic block 10 is provided at the bottom of the groove 8. The first electromagnetic block 9 cooperates with the second electromagnetic block 10, and the sealing is better and the operation is convenient.

[0020] The bottom end of the placement table 19 is fixedly connected to a rotating rod 32, and the rotating rod 32 extends into the base 2. A driven gear 18 is fixedly sleeved on the surface of the rotating rod 32. A forward and reverse motor 16 is provided on one side of the rotating rod 32. The forward and reverse motor 16 is installed at the bottom end of the inner cavity of the base 2. The output end of the forward and reverse motor 16 is connected to a driving gear 17. The driving gear 17 and the driven gear 18 are engaged with each other. An annular slider 21 is fixed to the bottom end of the placement table 19, and an annular slide groove 20 is opened at the upper end of the base 2. The annular slider 21 slides with the annular slide groove 20 to drive the culture dish to rotate and shake, making the device more stable.

[0021] When in use, the device is placed in a designated position, and the controller 31 is used to control the first hydraulic rod 12 to rise and fall, so that the device remains stable. The stem cell culture dish is placed in the heating tank 27, and the two second hydraulic rods 24 are activated by the controller 31 to drive the two clamps 25 to move in opposite directions to fix the culture dish. The first electromagnetic block 9 and the second electromagnetic block 10 cooperate to install the sealing chamber 3, and the forward and reverse motor 16 is activated by the controller 31 to drive the driving gear 17 to rotate. The driving gear 17 and the driven gear 18 are engaged with each other, driving the rotating rod 32 to rotate, driving the placement table 19 to rotate, and shaking the stem cell culture dish. The third hydraulic rod 24 is activated by the controller 31. Rod 29 drives the placement plate 22 to simulate an irregular fluid oscillation pattern, oscillating the stem cell culture dish so that the stem cells in the culture dish are evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of the stem cells and improving the culture effect. The temperature can be set by the controller 31, the temperature sensor 30 senses the temperature of the stem cell culture dish, and the heater 28 heats the stem cell culture dish. During the use of the device, vibrations will be generated. The damper 14 and the air spring 15 can effectively isolate external vibrations and buffer the vibrations of the device, ensuring the high stability of the entire device during operation and reducing the interference of external vibrations on the stem cell culture.

[0022] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising a base plate (1) and a controller (31), characterized in that: The upper end of the bottom plate (1) is provided with a base (2), the upper end of the base (2) is provided with a sealed chamber (3), the lower end of the bottom plate (1) is provided with a support leg (13), the upper end of the inner cavity of the support leg (13) is provided with a first hydraulic rod (12), the lower end of the first hydraulic rod (12) is located in the support leg (13), the telescopic end of the first hydraulic rod (12) is fixedly connected to the bottom end of the bottom plate (1), the bottom end of the first hydraulic rod (12) is fixedly connected to an air spring (15), dampers (14) are provided on both sides of the air spring (15), the lower ends of the two dampers (14) are fixedly connected to the bottom end of the inner cavity of the support leg (13), the upper ends of the two dampers (14) are fixedly connected to the bottom end of the first hydraulic rod (12), the sealed chamber (3) A placing table (19) is provided inside, and a placing plate (22) is rotatably connected to the placing table (19), and support plates (23) are fixedly connected to both sides of the upper ends of the two placing plates (22), and the inner ends of the two support plates (23) are fixedly provided with second hydraulic rods (24), and the telescopic ends of the two second hydraulic rods (24) are fixedly connected to clamps (25), and the inner ends of the two clamps (25) are provided with pressure sensors (26), and a heating groove (27) is provided between the two clamps (25), and the heating groove (27) is opened on the placing plate (22), and third hydraulic rods (29) are provided on both sides of the bottom end of the inner cavity of the placing plate (22), and the telescopic ends of the two third hydraulic rods (29) are fixedly connected to the bottom end of the placing plate (22).

2. A stem cell culture device according to claim 1, characterized in that: The sealed chamber (3) adopts a double-layer sealing structure, including an inner layer (6) and an outer layer (4), wherein the inner layer (6) is made of high-purity polytetrafluoroethylene material, and the outer layer (4) is made of hard and transparent polycarbonate material, and a vacuum insulation layer (5) is provided between the two layers.

3. The stem cell culture device according to claim 1, characterized in that: A first electromagnetic block (9) is provided at the lower end of the sealing chamber (3), a sealing gasket (7) is provided around the first electromagnetic block (9), a groove (8) is provided at the upper end of the base (2), a second electromagnetic block (10) is provided at the bottom end of the groove (8), and the first electromagnetic block (9) cooperates with the second electromagnetic block (10).

4. The stem cell culture device according to claim 1, characterized in that: The bottom end of the placement platform (19) is fixedly connected with a rotating rod (32), and the rotating rod (32) extends into the base (2). A driven gear (18) is fixedly sleeved on the surface of the rotating rod (32). A forward and reverse motor (16) is provided on one side of the rotating rod (32). The forward and reverse motor (16) is installed at the bottom end of the inner cavity of the base (2). The output end of the forward and reverse motor (16) is connected with a driving gear (17). The driving gear (17) and the driven gear (18) are meshed with each other. An annular slider (21) is fixedly provided at the bottom end of the placement platform (19). An annular slide groove (20) is provided at the upper end of the base (2). The annular slider (21) is slidably matched with the annular slide groove (20).

5. The stem cell culture device according to claim 1, characterized in that: Sound insulation cotton (11) is provided in the base (2).

6. The stem cell culture device according to claim 1, characterized in that: A heater (28) is provided at the lower end of the heating tank (27), and a temperature sensor (30) is provided in the heating tank (27).

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