Plunger type deep-sea organism cultivation device for in-situ environment remodeling

Abstract

The plunger type deep-sea organism cultivation device comprises a cultivation cabin, the upper portion and the lower portion of the cultivation cabin are each provided with a large opening, one side wall of the cultivation cabin is provided with a small opening, a plunger is installed between the two large openings in a matched mode, and the plunger is provided with a plurality of cabin penetrating openings and a water inlet and outlet connector. A biological transfer butt joint valve is mounted at the small opening; a flange ring is mounted at the top of the culture cabin, a base is mounted at the bottom of the culture cabin, and the culture cabin is connected with a temperature control system, so that zero-load sealing of a peripheral structure is realized; due to the integrated design, the temperature adjusting coil pipe is in direct contact with an internal medium, and the temperature adjusting efficiency is improved; the pressurizing temperature compensation coil pipe eliminates the influence of medium temperature change in the pressurizing and water changing process on a temperature field in the culture cabin, and comprehensive heat management is carried out in combination with the working process of the culture device.

Description

Technical Field

[0001] The present invention relates to the technical field of deep-sea organism cultivation devices, in particular to a plunger-type deep-sea organism cultivation device for in-situ environment remodeling. Background Art

[0002] With the advancement of deep-sea exploration technology, more and more deep-sea creatures are being discovered, constantly refreshing human cognition. Because deep-sea creatures have adapted to the extreme environment of the deep sea (pressure, temperature, etc.), conventional laboratory methods cannot be used to cultivate them. At present, there are two main ways to achieve the live cultivation of deep-sea organisms. One is to achieve "in situ" cultivation and observation in a deep-sea environment. The other is to establish a cultivation system suitable for the growth and survival of deep-sea organisms in a land laboratory. The first method is costly and risky. Establishing a deep-sea biological cultivation device in a land laboratory is one of the best options.

[0003] In existing technologies, incubation devices use flange bolts, threaded connections, or external clamps to seal the device. These rely on the pressure-bearing structure of the container's outer shell, requiring reinforced support, increasing manufacturing costs, and leading to stress concentration in bolt holes or at the contact points of the clips. Long-term use can easily induce metal fatigue and shorten service life. Furthermore, some existing incubation devices fail to consider temperature control, making it difficult to achieve long-term low (or high) temperature incubation. Some devices that do consider temperature control utilize coils or jackets arranged outside the chamber for temperature control. However, the walls of the devices under ultra-high pressure conditions (≥100MPa) are relatively thick, making external temperature control less efficient. Furthermore, existing incubation devices fail to consider the impact of changes in inlet water temperature due to pressurization and water replacement on the internal environment of the incubation chamber. Summary of the Invention

[0004] In response to the shortcomings of the above-mentioned existing production technologies, the applicant provides a plunger-type deep-sea biological cultivation device for in-situ environmental reshaping, thereby effectively solving problems such as reliance on the pressure-bearing and sealing of the container's peripheral structure, low temperature regulation efficiency, and the impact of changes in inlet water temperature caused by pressurization and water exchange on the internal environment of the culture chamber.

[0005] The technical solutions adopted in the present invention are as follows:

[0006] A plunger-type deep-sea organism cultivation device for in-situ environmental remodeling includes a culture chamber, wherein the upper and lower portions of the culture chamber are respectively provided with large openings, and a small opening is provided on one side wall of the culture chamber. A plunger is mounted between the two large openings, and the plunger is provided with multiple hatches and water inlet and outlet ports. A biological transfer docking valve is installed at the small opening.

[0007] A flange ring is installed at the top of the culture chamber, a base is installed at the bottom of the culture chamber, and the culture chamber is connected to a temperature control system;

[0008] The structure of the temperature control system is as follows: it includes a refrigeration unit, the outlet of the refrigeration unit is connected to the refrigerant buffer tank through a pipeline, and the refrigerant buffer tank is connected to the inlet of the refrigeration unit through a first circulation pump; the refrigerant buffer tank is also connected to the heat exchanger through a second circulation pump, and the heat exchanger returns to the refrigerant buffer tank; the refrigerant buffer tank is connected to the inlet of the temperature control coil through a third circulation pump, the temperature control coil is wound around the plunger, and the outlet of the temperature control coil returns to the refrigerant buffer tank; the heat exchanger is also connected to the water inlet of the plunger through a pipeline, and the heat exchanger is connected to the water exchange port of the plunger through a fourth circulation pump; it also includes a water tank, which is also connected in parallel with the heat exchanger through a fourth circulation pump and a pipeline, and the water tank is also connected to the pressure control system.

[0009] Its further technical solution is:

[0010] The culture cabin adopts an integrated structure, the main body of the culture cabin is a spherical structure, and heat insulation materials are arranged outside the culture cabin.

[0011] A plurality of top screw holes and bottom threaded holes are respectively reserved on the top and bottom of the culture chamber.

[0012] The structure of the plunger is as follows: the upper and lower covers of the plunger are respectively provided with an upper cover sealing groove and a lower cover sealing groove, the upper cover of the plunger is provided with a water inlet for injecting water and feeding bait; the upper cover of the plunger is also provided with a pressure measuring port and a temperature measuring port for installing a pressure sensor and a temperature sensor; the upper cover of the plunger is also provided with a camera hatch and a lighting hatch for installing a camera and a lighting; the upper cover of the plunger is provided with screw holes for installing the plunger lifting ears; the lower cover of the plunger is provided with a water exchange port for changing water and discharging sewage.

[0013] The plunger also has reserved temperature control coil inlet and outlet interfaces for installing the temperature control coil in the temperature control system; the plunger lower cover has reserved interfaces for expanding functions.

[0014] The cross-sectional area of ​​the upper cover and the lower cover of the plunger is the same.

[0015] A plurality of first mounting holes are evenly distributed in the circumferential direction of the flange ring, and culture chamber lifting ears are symmetrically mounted on the flange ring.

[0016] The cross section of the base is a "convex"-shaped structure, and an inspection port, a cleaning water tank, a drainage pipe and a plurality of second installation holes are provided on the base.

[0017] A pressurized temperature compensation coil is arranged inside the refrigerant buffer box. The inlet of the pressurized temperature compensation coil is connected to the outlet of the pressure control system, and the outlet is connected to the water injection port.

[0018] A heater is installed in the refrigerant buffer tank for high and low temperature adjustment.

[0019] The beneficial effects of the present invention are as follows:

[0020] The present invention has a compact and reasonable structure and is easy to operate. Sealing is achieved by arranging plungers at the upper and lower large openings of the culture cabin, and the cross-sectional areas of the upper and lower covers of the plunger are equal. When the interior of the cabin is under pressure, the upper and lower covers of the plunger are subjected to force in opposite directions and are equal in size. The plunger is in a self-balanced state under all pressures, and the plunger achieves sealing under zero external load conditions without the need for container structure support. The interfaces and functions required inside the device are all arranged on the plunger, and the device has a high degree of integration. At the same time, the temperature control coil is arranged on the plunger, and the integrated design is easy to install and maintain. The coil is in direct contact with the internal medium during temperature adjustment and insulation, thereby improving the temperature control efficiency. In addition, a pressurization temperature compensation coil is provided, which can eliminate the influence of medium temperature changes caused by pressurization of the pressure control system during pressurization and water change, and comprehensive thermal management is performed in combination with the work flow of the cultivation device.

[0021] The present invention achieves mutual cooperation among components such as a culture chamber, a plunger, a flange ring, a base, a temperature control system, a biological transfer docking valve, a clamp, a sealing ring and a pressure control system, and assembles the culture chamber, the plunger and the sealing ring to form a biological culture space. The remaining components are used to meet the installation and use of the device. A large opening is provided at the upper and lower parts of the culture chamber, and a small opening is provided at the equator. The plunger is inserted into the large opening of the culture chamber. The plunger is provided with multiple hatches for functional integration and is also wrapped with a temperature control coil for temperature control. The cross-sectional areas of the upper and lower covers of the plunger are equal. Under the pressure condition of the culture chamber, it maintains a self-balancing state, realizing zero-load sealing of the peripheral structure; the integrated design enables the temperature control coil to be in direct contact with the internal medium, thereby improving the temperature control efficiency; the pressurization temperature compensation coil eliminates the influence of the medium temperature change during the pressurization and water exchange process on the temperature field in the culture chamber, and performs comprehensive thermal management in combination with the work flow of the culture device. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the present invention.

[0023] Figure 2 for Figure 1 Partial view of (a).

[0024] Figure 3 for Figure 1 Partial view of (2).

[0025] Figure 4 It is a structural schematic diagram of the culture chamber of the present invention.

[0026] Figure 5 This is a structural schematic diagram of the culture chamber of the present invention from another perspective.

[0027] Figure 6 It is a schematic structural diagram of the plunger of the present invention.

[0028] Figure 7 It is a front view of the plunger of the present invention.

[0029] Figure 8 for Figure 7 Top view of .

[0030] Figure 9 for Figure 7 Bottom view of .

[0031] Figure 10 It is a structural schematic diagram of the flange ring of the present invention.

[0032] Figure 11 It is a structural schematic diagram of the base of the present invention.

[0033] Including: 1. Culture chamber; 2. Plunger; 3. Flange ring; 4. Base; 5. Temperature control system; 6. Biological transfer docking valve; 7. Top screw hole; 8. Bottom threaded hole; 9. Clamp; 10. Upper cover sealing ring; 11. Lower cover sealing ring; 12. Pressure control system

[0034] 201, upper cover sealing groove; 202, lower cover sealing groove; 203, water filling port; 204, pressure measuring port; 205, temperature measuring port; 206, camera hatch; 207, lighting hatch; 208, screw hole; 209, plunger lifting lug; 210, water exchange port; 211, thermostat coil inlet port; 212, thermostat coil outlet port; 213, reserved port;

[0035] 301, culture chamber hanging ear; 302, first mounting hole;

[0036] 401, inspection port; 402, water tank for cleaning; 403, drainage pipe; 404, second mounting hole;

[0037] 501. Refrigeration unit; 502. Refrigerant buffer tank; 503. Heat exchanger; 504. Temperature control coil; 505. Insulation material; 506. First circulation pump; 507. Second circulation pump; 508. Third circulation pump; 509. Fourth circulation pump; 510. Pressurized temperature compensation coil; 511. Water tank; 512. First high-pressure stop valve; 513. Second high-pressure stop valve.

[0038] 50201, first inlet of buffer box; 50202, first outlet of buffer box; 50203, second outlet of buffer box; 50204, second inlet of buffer box; 50205, third outlet of buffer box; 50206, third inlet of buffer box; 50207, heater. DETAILED DESCRIPTION

[0039] The specific embodiments of the present invention will be described below with reference to the accompanying drawings.

[0040] like Figures 1-11As shown, the plunger-type deep-sea organism cultivation device for in-situ environmental remodeling of this embodiment includes a culture chamber 1, with large openings at the upper and lower portions of the culture chamber 1, a small opening at one side wall of the culture chamber 1, a plunger 2 mounted between the two large openings, multiple hatches and water inlet and outlet ports provided on the plunger 2, and an organism transfer docking valve 6 mounted at the small opening;

[0041] A flange ring 3 is installed at the top of the culture chamber 1, a base 4 is installed at the bottom of the culture chamber 1, and the culture chamber 1 is connected to a temperature control system 5;

[0042] The structure of the temperature control system 5 is as follows: it includes a refrigeration unit 501, the outlet of the refrigeration unit 501 is connected to the coolant buffer tank 502 through a pipeline, and the coolant buffer tank 502 is connected to the inlet of the refrigeration unit 501 through a first circulation pump 506; the coolant buffer tank 502 is also connected to the heat exchanger 503 through a second circulation pump 507, and the heat exchanger 503 returns to the coolant buffer tank 502; the coolant buffer tank 502 is connected to the temperature control coil 501 through a third circulation pump 508. 4 inlet connection, the temperature control coil 504 is wound around the plunger 2, and the outlet of the temperature control coil 504 is returned and connected to the coolant buffer tank 502; the heat exchanger 503 is also connected to the water injection port 203 of the plunger 2 through a pipeline, and the heat exchanger 503 is connected to the water exchange port 210 of the plunger 2 through a fourth circulation pump 509; and also includes a water tank 511, which is also connected in parallel with the heat exchanger 503 through the fourth circulation pump 509 and the pipeline, and the water tank 511 is also connected to the pressure control system 12.

[0043] The culture chamber 1 adopts an integrated structure. The main body of the culture chamber 1 is a spherical structure. A heat-insulating material 505 is arranged outside the culture chamber 1 .

[0044] A plurality of top screw holes 7 and bottom threaded holes 8 are respectively reserved on the top and bottom of the culture chamber 1 .

[0045] The structure of the plunger 2 is as follows: the upper and lower covers of the plunger 2 are respectively provided with an upper cover sealing groove 201 and a lower cover sealing groove 202, and the upper cover of the plunger 2 is provided with a water injection port 203 for injecting water and feeding; the upper cover of the plunger 2 is also provided with a pressure measuring port 204 and a temperature measuring port 205 for installing a pressure sensor and a temperature sensor; the upper cover of the plunger 2 is also provided with a camera hatch 206 and a lighting hatch 207 for installing a camera and a lighting; the upper cover of the plunger 2 is provided with a screw hole 208 for installing a plunger lifting ear 209; the lower cover of the plunger 2 is provided with a water exchange port 210 for changing water and discharging sewage.

[0046] The plunger 2 also reserves a temperature control coil inlet interface 211 and a temperature control coil outlet interface 212 for installing the temperature control coil 504 in the temperature control system 5; the lower cover of the plunger 2 reserves an interface 213 for expanding functions.

[0047] The cross-sectional area of ​​the upper cover and the lower cover of the plunger 2 is the same.

[0048] A plurality of first mounting holes 302 are evenly distributed in the circumferential direction of the flange ring 3 , and culture chamber hanging ears 301 are symmetrically mounted on the flange ring 3 .

[0049] The cross section of the base 4 is a convex structure, and the base 4 is provided with an inspection port 401 , a cleaning water tank 402 , a drainage pipe 403 and a plurality of second mounting holes 404 .

[0050] A pressurized temperature compensating coil 510 is arranged inside the coolant buffer tank 502 . The inlet of the pressurized temperature compensating coil 510 is connected to the outlet of the pressure control system 12 , and the outlet is connected to the water injection port 203 .

[0051] A heater 50207 is provided in the coolant buffer tank 502 for adjusting the high and low temperatures.

[0052] like Figure 1-Figure 3 As shown, the specific structure and functions of the plunger-type deep-sea organism cultivation device for in-situ environment remodeling of this embodiment are as follows:

[0053] The culture chamber 1 comprises a culture chamber 1, the main body of which is a spherical structure. A large opening is respectively provided at the upper and lower parts of the culture chamber 1, and a small opening is provided at the equator of the spherical shell of the culture chamber 1.

[0054] The upper and lower large openings of the culture chamber 1 are provided with plungers 2, and the plunger 2 is provided with multiple hatches and water inlet and outlet interfaces.

[0055] A flange ring 3 is installed on the top of the culture chamber 1 , and a culture chamber hanging ear 301 and a first mounting hole 302 are symmetrically arranged on the flange ring 3 .

[0056] A base 4 is installed at the bottom of the culture chamber 1 , and the base 4 is provided with an inspection port 401 , a cleaning water tank 402 , a drainage pipe 403 and a plurality of second installation holes 404 .

[0057] The culture chamber 1 is connected to a temperature control system 5, which includes a refrigeration unit 501, a coolant buffer tank 502, a heat exchanger 503, a temperature control coil 504, an insulation material 505, a first circulation pump 506, a second circulation pump 507, a third circulation pump 508, a fourth circulation pump 509, a boost temperature compensation coil 510 and a water tank 511.

[0058] The small opening at the equator of the culture chamber 1 is connected to a biological transfer docking valve 6 .

[0059] A plurality of top screw holes 7 and bottom threaded holes 8 are respectively reserved on the top and bottom of the culture chamber 1 .

[0060] like Figure 6 and Figure 7As shown, the plunger 2 is a cylindrical I-shaped structure. The upper and lower covers of the plunger 2 are respectively provided with an upper cover sealing groove 201 and a lower cover sealing groove 202. The upper cover of the plunger 2 is provided with a water inlet 203 for water injection and bait feeding. The upper cover of the plunger 2 is also provided with a pressure measuring port 204 and a temperature measuring port 205 for installing a pressure sensor and a temperature sensor. The upper cover of the plunger 2 is also provided with a camera through hatch 206 and a light through hatch 207 for installing a camera and a light. The upper cover of the plunger 2 is provided with a screw hole 208 for installing a plunger lifting lug 209. The lower cover of the plunger 2 is provided with a water exchange port 210 for water exchange and sewage discharge. The plunger 2 also reserves a thermostat coil inlet interface 211 and a thermostat coil outlet interface 212 for installing the thermostat coil 504 in the temperature control system 5. The lower cover of the plunger 2 reserves an interface 213 for expanding functions.

[0061] like Figure 4 As shown, two culture chamber hanging ears 301 are arranged on the flange ring 3 at intervals of 180 degrees, and six first mounting holes 302 are arranged at intervals of 60 degrees. The flange ring 3 is connected to the culture chamber 1 by installing screws in the first mounting holes 302 .

[0062] like Figure 1 As shown, the biological transfer docking valve 6 is connected to the culture chamber 1 through a clamp 9. The clamp 9 consists of two pieces and can be disassembled and assembled by bolts.

[0063] like Figure 1 、 Figure 3 As shown, the outlet of the refrigeration unit 501 in the temperature control system 5 is connected to the first inlet 50201 of the buffer tank of the coolant buffer tank 502 through a pipeline, and the first outlet 50202 of the buffer tank of the coolant buffer tank 502 is connected to the inlet of the refrigeration unit 501 through a first circulation pump 506; the second outlet 50203 of the buffer tank of the coolant buffer tank 502 is connected to the primary side inlet of the heat exchanger 503 through a second circulation pump 507, and the second inlet of the buffer tank of the coolant buffer tank 502 is connected to the primary side inlet of the heat exchanger 503. The port 50204 is connected to the primary side outlet of the heat exchanger 503 through a pipeline; the third buffer tank outlet 50205 of the refrigerant buffer tank 502 is connected to the inlet of the temperature control coil 504 through the third circulation pump 508, and the third buffer tank inlet 50206 of the refrigerant buffer tank 502 is connected to the outlet of the temperature control coil 504 through a pipeline; the secondary side outlet of the heat exchanger 503 is connected to the water injection port 203 through a pipeline, and the secondary side inlet is connected to the water exchange port 210 through the fourth circulation pump 509.

[0064] like Figure 6 As shown, the cross-sectional areas of the upper and lower covers of the plunger 2 are equal.

[0065] The upper cover sealing groove 201 and the lower cover sealing groove 202 on the plunger 2 are sealed with an upper cover sealing ring 10 and a lower cover sealing ring 11. The inner wall of the culture chamber 1, the plunger 2, the upper cover sealing ring 10 and the lower cover sealing ring 11 are assembled to form a closed space.

[0066] like Figure 1 As shown, the temperature control system 5 is further provided with a water tank 511, which is also connected in parallel with the heat exchanger 503 through the fourth circulation pump 509 and the pipeline. The fourth circulation pump 509 is shared to reduce the temperature of the water in the water tank 511. The water tank 511 is also connected to the pressure control system 12 to supply hot and cold water for pressurization and water exchange.

[0067] The temperature control system 5 is further provided with a pressurized temperature compensating coil 510 placed in the coolant buffer tank 502 . The inlet of the pressurized temperature compensating coil 510 is connected to the outlet of the pressure control system 12 , and the outlet is connected to the water inlet 203 .

[0068] like Figure 1 、 Figure 3 As shown, a heater 50207 is provided in the coolant buffer tank 502 for adjusting the high and low temperatures; a temperature regulating coil 504 is spirally wound around the plunger 2 and is a pressure-resistant coil; and a heat-insulating material 505 is arranged outside the culture chamber 1.

[0069] The working principle and actual working implementation process of this embodiment are as follows:

[0070] In this embodiment, a plunger 2 is installed inside the culture chamber 1 to achieve sealing. The cross-sectional areas of the upper and lower covers of the plunger 2 are consistent. When the culture chamber 1 is pressurized, the upper and lower covers of the plunger 2 are subjected to equal and opposite forces, causing the plunger 2 to be in a self-balanced state under all pressures. No external load is required to be applied by the peripheral structure to achieve sealing between the culture chamber 1 and the plunger 2. The plunger 2 is inserted into the culture chamber 1, and various interfaces can be integrated on the plunger 2. At the same time, a temperature control coil 504 is integrated to achieve direct contact between the medium inside the culture chamber 1 and the temperature control coil 504, thereby improving heat transfer and insulation efficiency. The water tank 511 provides hot and cold water for pressurization and water exchange. However, the pressurization process will cause the medium temperature to change. Before entering the culture chamber 1, the pressurized medium is first temperature-controlled by heat exchange with the refrigerant through the pressurization temperature compensation coil 510. The medium enters the culture chamber 1 at the target temperature, eliminating the impact of the pressurization and water exchange processes on the temperature field inside the culture chamber 1.

[0071] In actual work process:

[0072] First, secure the incubation chamber 1 to the base 4 and install the insulation material 505. Install the upper cover sealing ring 10 and the lower cover sealing ring 11 into the upper cover sealing groove 201 and the lower cover sealing groove 202. Then, install the temperature control coil 504 onto the plunger 2, connecting the coil inlet and outlet to the temperature control coil inlet port 211 and the temperature control coil outlet port 212 on the plunger 2, respectively. Install the pressure sensor, temperature sensor, camera, and lighting at the pressure gauge port 204, temperature gauge port 205, camera hatch 206, and lighting hatch 207 on the plunger 2, respectively. Next, lower the plunger 2 into the incubation chamber 1 using the plunger lifting lug 209. Secure the flange ring 3 to the incubation chamber 1, and connect the temperature control system 5 to the incubation chamber 1 using the piping. Next, fill the incubation chamber 1 with water through the water inlet 203. Turn on the temperature control system 5 and set the target temperature to rapidly cool or heat the coolant. Once the culture chamber 1 is filled with water, the second, third, and fourth circulation pumps 507, 508, and 509 are turned on. The medium in the culture chamber 1 exchanges heat with the coolant through the heat exchanger 503 and the temperature-regulating coil 504. After the temperature is adjusted to the target temperature, the external atmospheric pressure line is shut off via the first and second high-pressure shut-off valves 512, 513, stopping circulation within the culture chamber 1. The fourth circulation pump 509 is then connected to the water tank 511, allowing the water in the tank 511 to be heated and supplied for pressurization and water exchange. The third circulation pump 508 is kept running, and the temperature-regulating coil 504 is used to neutralize heat loss to the environment. Water is then drawn from the water tank 511 and pumped into the culture chamber 1 via the pressure control system 12 for pressurization. Before entering the culture chamber 1, the pressurized medium passes through the pressurization temperature compensation coil 510 to eliminate temperature fluctuations caused by the pressurization process. Once the target environment is reached, the organisms to be cultured are transferred to the culture chamber 1 for long-term cultivation via the organism transfer docking valve 6. During the cultivation period, feed and water are added through the water injection port 203 and the water exchange port 210, and the temperature is maintained stable through the temperature regulating coil 504.

[0073] In this embodiment, plungers 2 are arranged at the upper and lower large openings of the culture chamber to achieve sealing, and the cross-sectional areas of the upper and lower covers of the plunger 2 are equal. When the interior of the chamber is under pressure, the upper and lower covers of the plunger 2 are subjected to forces in opposite directions and of equal magnitude. The plunger 2 is in a self-balanced state under all pressures, and the plunger 2 achieves sealing under zero external load conditions without the need for a container structure to bear the load. The interfaces and functions required inside the device are all provided on the plunger 2, and the device has a high degree of integration. At the same time, the temperature control coil 504 is arranged on the plunger 2, and the integrated design is convenient for installation and maintenance. The coil is in direct contact with the internal medium during temperature adjustment and insulation, thereby improving the temperature control efficiency. In addition, a pressurization temperature compensation coil 510 is provided. When pressurizing and changing water, the influence of medium temperature changes caused by pressurization of the pressure control system 12 can be eliminated, and comprehensive thermal management is performed in combination with the work flow of the culture device.

[0074] The above description is an explanation of the present invention, not a limitation of the present invention. The scope of the present invention is defined in the claims. Any modifications may be made within the scope of protection of the present invention.

Claims

1. A plunger-type deep-sea organism cultivation device for in-situ environmental remodeling, characterized by: The culture chamber (1) comprises a culture chamber (1), wherein the upper and lower parts of the culture chamber (1) are respectively provided with large openings, a side wall of the culture chamber (1) is provided with a small opening, a plunger (2) is installed between the two large openings, a plurality of hatch openings and water inlet and outlet interfaces are provided on the plunger (2), and a biological transfer docking valve (6) is installed at the small opening; A flange ring (3) is installed at the top of the culture chamber (1), a base (4) is installed at the bottom of the culture chamber (1), and the culture chamber (1) is connected to a temperature control system (5); The structure of the temperature control system (5) is as follows: it includes a refrigeration unit (501), the outlet of the refrigeration unit (501) is connected to the coolant buffer tank (502) through a pipeline, the coolant buffer tank (502) is connected to the inlet of the refrigeration unit (501) through a first circulation pump (506); the coolant buffer tank (502) is also connected to the heat exchanger (503) through a second circulation pump (507), and the heat exchanger (503) returns to the coolant buffer tank (502); the coolant buffer tank (502) is connected to the temperature control coil (504) through a third circulation pump (508). The plunger (2) is connected to the heat exchanger (503) through a pipe, the temperature regulating coil (504) is wound around the plunger (2), and the outlet of the temperature regulating coil (504) is returned and connected to the coolant buffer tank (502); the heat exchanger (503) is also connected to the water injection port (203) of the plunger (2) through a pipe, and the heat exchanger (503) is connected to the water exchange port (210) of the plunger (2) through a fourth circulation pump (509); and the water tank (511) is also connected in parallel with the heat exchanger (503) through the fourth circulation pump (509) and the pipe, and the water tank (511) is also connected to the pressure control system (12).

2. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: The culture chamber (1) adopts an integrated structure, the main body of the culture chamber (1) is a spherical structure, and a heat-insulating material (505) is arranged outside the culture chamber (1).

3. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: The top and bottom of the culture chamber (1) are respectively provided with a plurality of top screw holes (7) and bottom threaded holes (8).

4. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: The structure of the plunger (2) is as follows: the upper and lower covers of the plunger (2) are respectively provided with an upper cover sealing groove (201) and a lower cover sealing groove (202); the upper cover of the plunger (2) is provided with a water injection port (203) for injecting water and feeding; the upper cover of the plunger (2) is also provided with a pressure measuring port (204) and a temperature measuring port (205) for installing a pressure sensor and a temperature sensor; the upper cover of the plunger (2) is also provided with a camera through hatch (206) and a lighting through hatch (207) for installing a camera and a lighting; the upper cover of the plunger (2) is provided with a screw hole (208) for installing a plunger lifting lug (209); and the lower cover of the plunger (2) is provided with a water exchange port (210) for exchanging water and discharging sewage.

5. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 4, characterized in that: The plunger (2) also reserves a temperature control coil inlet interface (211) and a temperature control coil outlet interface (212) for installing the temperature control coil (504) in the temperature control system (5); the plunger (2) lower cover reserves an interface (213) for expanding functions.

6. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 5, characterized in that: The cross-sectional areas of the upper cover and the lower cover of the plunger (2) are the same.

7. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: The flange ring (3) is evenly distributed with a plurality of first mounting holes (302) in the circumferential direction, and the flange ring (3) is symmetrically mounted with culture chamber hanging ears (301).

8. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: The cross section of the base (4) is in a "convex" shape, and the base (4) is provided with an inspection port (401), a cleaning water tank (402), a drainage pipe (403) and a plurality of second mounting holes (404).

9. The plunger-type deep-sea organism cultivation device for in-situ environmental remodeling according to claim 1, characterized in that: A pressurized temperature compensation coil (510) is arranged inside the coolant buffer tank (502), the inlet of the pressurized temperature compensation coil (510) is connected to the outlet of the pressure control system (12), and the outlet is connected to the water injection port (203).

10. The plunger-type deep-sea organism cultivation device for in-situ environment remodeling according to claim 1, characterized in that: A heater (50207) is provided in the coolant buffer tank (502) for adjusting high and low temperatures.

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