Panlong jade lithospermum gel as well as preparation method and application thereof

Abstract

The invention relates to pan dragon jade and lithospermum gel as well as a preparation method and application thereof. The pan dragon jade and lithospermum gel is prepared from the following components in parts by weight: 20 to 50 parts of lithospermum oil mixed oil phase, 1 to 25 parts of pan dragon jade powder, 10 to 40 parts of surfactant, 2 to 8 parts of gel matrix, 200 to 700 parts of water phase, 25 to 75 parts of humectant, 1 to 5 parts of pH regulator and 0.5 to 2 parts of preservative, the lithospermum oil mixed oil phase is composed of lithospermum oil and a first oil phase, the mass ratio of the lithospermum oil to the first oil phase is 1: (0-10), and the lithospermum oil is prepared by wetting segment-shaped or granular lithospermum with a second oil phase, dipping and percolating. The oil-phase percolate is directly adopted as a target medicine to prepare the medicine-carrying oil phase, pan dragon jade powder is adopted as a mineral medicine, the advantages of the oil-phase percolate and the pan dragon jade powder are combined, and the gel which is good in stability, moderate in viscosity and easy to coat is obtained.

Description

Technical Field

[0001] The invention relates to Panlongyu Lithospermum officinale gel and a preparation method and application thereof, belonging to the field of traditional Chinese medicine preparations. Background Art

[0002] Lithospermum officinale is a traditional Chinese medicine, often used clinically for external treatment of skin diseases. Lithospermum officinale drugs mostly refer to preparations made with the Chinese herbal medicine Lithospermum officinale as the main ingredient, including oils, sprays, ointments, suppositories, etc., especially oils and ointments. Lithospermum officinale and Lithospermum officinale preparations have been widely used in the clinical treatment of diseases such as eczema, atopic dermatitis, skin wounds and mucosal injuries, psoriasis, common warts, and condyloma acuminatum. In addition, it has been found that lithospermum officinale drugs have the effects of cooling blood and activating blood circulation, detoxifying and clearing rashes when applied externally. They are widely used in traditional Chinese medicine dermatology and can treat burns, pressure sores, psoriasis, eczema, skin ulcers, dermatitis and other skin diseases. Naphthoquinone components are the main active ingredients of Lithospermum officinale. However, naphthoquinone components are extremely unstable and are greatly affected by temperature. Among them, shikonin is thermally unstable, so the percolation cold soaking extraction method is of great significance to improving the stability of shikonin.

[0003] The quartz, mica, chlorite, and calcite contained in Panlong jade have all been used as traditional Chinese medicine in classical medical texts. The Compendium of Materia Medica states that mica has the properties of calming the mind, relieving convulsions, promoting qi and relieving phlegm, and stopping bleeding and healing sores. It can be used to treat palpitations, insomnia, bleeding from wounds, carbuncles, ulcers, and eczema. The Shennong Herbal Classic states that white quartz is sweet, slightly warm, and can relieve rheumatism. The Mingyi Bielu describes purple quartz as having the ability to dissipate carbuncle swelling.

[0004] As the primary physical barrier between the human body and the outside world, the skin frequently suffers varying degrees of damage, necessitating wound healing and regeneration. From minor oral ulcers to major surgical wounds, and even amputations caused by intractable ulcers caused by diabetes, the importance of wound-healing drugs is evident. Therefore, developing effective, stable wound-healing drugs that both protect the wound surface and promote skin repair is of great medical significance. Gels are pharmaceuticals made from a combination of a drug and a gel-forming excipient. In addition to the therapeutic benefits of the drug itself, they also offer ease of use, ease of absorption, and long-lasting efficacy. Summary of the Invention

[0005] The purpose of the present invention is to provide a Panlongyu Lithospermum officinale gel and a preparation method and application thereof.

[0006] In a first aspect, the present invention provides a Panlongyu lithospermum gel, which comprises, by weight, 20 to 50 parts of a lithospermum oil mixed oil phase, 1 to 25 parts of Panlongyu powder, 10 to 40 parts of a surfactant, 2 to 8 parts of a gel matrix, 200 to 700 parts of an aqueous phase, 25 to 75 parts of a moisturizer, 1 to 5 parts of a pH regulator, and 0.5 to 2 parts of a preservative; the lithospermum oil mixed oil phase consists of lithospermum oil and a first oil phase, wherein the mass ratio of the lithospermum oil to the first oil phase is 1:(0 to 10), and the lithospermum oil is prepared by wetting and impregnating segmented or granular lithospermum with a second oil phase and then percolating.

[0007] Preferably, the Panlong jade powder is Panlong green jade powder and / or Panlong black jade powder, and the particle size of the Panlong jade powder is 80nm~200 μm.

[0008] Preferably, the first oil phase and / or the second oil phase is vegetable oil. Preferably, the vegetable oil is at least one of sesame oil, soybean oil, corn oil, olive oil, jojoba oil and peanut oil.

[0009] Preferably, the surfactant is at least one of Tween 80, Span 80, and polyvinyl alcohol (PVA); the gel matrix is ​​at least one of carbomer, methylcellulose, and sodium carboxymethylcellulose; the aqueous phase is a phosphate buffer solution or distilled water; the humectant is at least one of glycerol, sodium hyaluronate, trehalose, and hydroxypropyl methylcellulose; the pH adjuster is triethanolamine or sodium hydroxide; and the preservative is at least one of parabens, benzoates, sorbates, and propylene glycol.

[0010] In a second aspect, the present invention provides a method for preparing the above-mentioned Panlongyu Lithospermum officinale gel, comprising: 1) Cutting or crushing the lithospermum erythrorhizon into sections, wetting with a second oil phase, impregnating the lithospermum erythrorhizon, and then percolating to obtain lithospermum erythrorhizon oil; 2) mixing the lithospermum oil with the first oil phase to obtain the lithospermum oil mixed oil phase; 3) Grinding the gel matrix and moisturizer into fine powder, adding water and mixing evenly to fully swell the gel matrix; 4) The lithospermum oil mixed oil phase obtained in step 2) and the gel matrix obtained in step 3) are mixed evenly, and then Panlongyu powder is added and mixed evenly. Then, a surfactant, a pH adjuster and a preservative are added in sequence and mixed evenly to obtain the Panlongyu lithospermum gel.

[0011] Preferably, in step 1), the mass ratio of the lithospermum officinale to the second oil phase is 1:(2-5).

[0012] Preferably, in step 1), the wetting and soaking time is 2 to 10 days.

[0013] In a third aspect, the present invention provides a use of the above-mentioned Panlongyu Lithospermum gel in the preparation of medicines for treating burns, pressure sores, psoriasis, eczema, skin ulcers, and dermatitis.

[0014] The Panlongyu Lithospermum gel provided by the present invention is a combination of Panlongyu jade and Lithospermum erythrorhizon, with quartz, mica, calcite, and Lithospermum erythrorhizon extract as active ingredients. It has significant hemostatic, sore-healing, and rash-relieving effects. The resulting gel has a uniform, smooth, lustrous appearance, moderate viscosity, is easy to apply, and exhibits good in vitro drug release. Compared with traditional methods for preparing gels, the present invention directly uses oil-phase percolation as the target drug to produce the drug-loaded oil phase, and uses Panlongyu powder as the mineral drug. This simplifies the preparation process and combines the advantages of both to produce a stable, easily controllable, moderately viscous, and easily applied gel, enhancing its efficacy and user experience. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for describing the embodiments.

[0016] Figure 1 The following is the appearance and properties of the lithospermum oil prepared according to Example 1 and the HPLC spectrum of the index component shikonin.

[0017] Figure 2 The present invention shows the Panlongyu Lithospermum officinale gel prepared according to Example 2 and its appearance and properties.

[0018] Figure 3 The present invention discloses the Panlong Black Jade Lithospermum Gel prepared according to Example 3 and its appearance and properties.

[0019] Figure 4 The appearance and properties of the lithospermum gel prepared at different ratios of lithospermum oil and peanut oil in the lithospermum oil mixed oil phase in Comparative Example 1 are shown.

[0020] Figure 5 The present invention discloses the Panlong black jade lithospermum gel and its appearance properties with an excess of Panlong black jade powder added according to comparative example 2.

[0021] Figure 6 The following are the Panlong jade gels and their appearance properties obtained according to Comparative Example 3 with different amounts of Panlong jade powder added.

[0022] Figure 7 The HPLC spectrum of shikonin, an indicator component in the Panlong Black Jade Lithospermum Gel prepared according to Example 3.

[0023] Figure 8 The in vitro anti-inflammatory activity of the Panlong Black Jade Lithospermum Gel prepared according to Example 3. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only some embodiments, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of this application. In addition, it should be understood that the specific embodiments described herein are only used to illustrate and explain the present invention and are not intended to limit the present invention.

[0025] The invention prepares the Panlongyu-lithospermum compound gel by mixing lithospermum erythrorhizon and Panlongyu powder.

[0026] First, prepare lithospermum oil: Lithospermum erythrorhizon was purchased from Shanghai Kangqiao Chinese Medicine Pieces Co., Ltd. The lithospermum was cut into small segments approximately 1 cm in size, moistened with an oil phase, and soaked for several days before percolation to obtain the lithospermum percolate (lithospermum oil). It should be understood that the lithospermum can also be crushed into granules. The oil phase can be a vegetable oil, such as at least one of sesame oil, soybean oil, corn oil, olive oil, jojoba oil, and peanut oil. The amount of oil phase used for moistening and soaking can be 2 to 5 times the amount of lithospermum erythrorhizon. The oil phase can be added to the lithospermum all at once, or a portion can be added first to moisten the lithospermum, left to stand for a period of time, and then the remaining oil phase can be added to continue soaking. The number of days for moistening and soaking can range from 2 to 10 days.

[0027] The lithospermum oil can be further compounded with the oil phase to form lithospermum composite oil, and the weight ratio of the lithospermum oil to the oil phase in the lithospermum composite oil can be 1: (0-10).

[0028] Panlong jade powder is produced in the Longgang Mountains of Panshi City, Jilin Province. It is at least one of Panlong green jade (yellow-green) or Panlong black jade (black). The Panlong green jade powder or Panlong black jade powder used in this application is provided by Jilin Panlong Jade Development Co., Ltd. Before use, it is ground and sieved to a particle size of 80 nm to 200 µm. Preferably, the particle size of the Panlong jade powder is 80 to 500 nm.

[0029] Preparation of the gel matrix: Add the gel matrix to the moisturizer, grind thoroughly, and add an aqueous phase to swell the mixture to form a gel matrix. The gel matrix can be at least one of carbomer, methylcellulose, and sodium carboxymethylcellulose. The moisturizer can be at least one of glycerin, sodium hyaluronate, trehalose, and hydroxypropyl methylcellulose. The aqueous phase can be a phosphate buffer solution or distilled water.

[0030] The aforementioned lithospermum oil or lithospermum compound oil and a surfactant are added to the gel matrix and mixed thoroughly before adding the Panlongyu powder. A pH adjuster is added to adjust the pH to 6.8-7.2. After stirring thoroughly, an appropriate amount of preservative is added. The surfactant can be at least one of Tween 80, Span 80, and polyvinyl alcohol (PVA). The pH adjuster can be triethanolamine or sodium hydroxide. The preservative can be at least one of parabens, benzoates, sorbates, and propylene glycol.

[0031] The components of the Panlongyu and Lithospermum compound gel of the present invention may be composed of 20 to 50 parts of a mixed oil phase of lithospermum oil, 1 to 25 parts of Panlongyu powder, 10 to 40 parts of a surfactant, 2 to 8 parts of a gel base, 200 to 700 parts of an aqueous phase, 25 to 75 parts of a moisturizer, 1 to 5 parts of a pH regulator, and 0.5 to 2 parts of a preservative.

[0032] Preferably, the weight ratio of the lithospermum oil mixed oil phase to the Panlong jade powder is 20:(1-10). More preferably, the weight ratio of the lithospermum oil mixed oil phase to the Panlong jade powder is 20:(1-4).

[0033] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods. Unless otherwise specified, the materials and reagents used in the following examples are all commercially available. In the following examples, an SPD-20AD high performance liquid chromatograph (Shimadzu, Japan), a Varioskan Flash full wavelength scanning multifunctional reader (ThermoFisher, USA), a BSA-124S-CW analytical balance (Sartorius, Germany), an Eppendorf 5430 centrifuge (Eppendorf, Germany), and an S23-2 constant temperature magnetic stirrer (Shanghai Silo Instrument Co., Ltd., China) were used.

[0034] Example 1 Preparation of Lithospermum officinale oil Weigh the lithospermum root material, cut it into small pieces of about 1 cm, fully moisten it with 2 times the weight of peanut oil in a beaker, place it in a sealed container for 12 hours, then add 1 times the weight of peanut oil and soak it for 7 days, then filter it to obtain lithospermum oil for later use.

[0035] Example 2 Take 1 g of glycerol and add 0.1 g of carbomer 940 to grind finely, add 10 mL of distilled water to swell it, add 1 g of lithospermum oil and 0.2 g of Tween 80 to the above mixture, add 0.5 g of Panlong black jade powder / Panlong green jade powder (200 mesh), add triethanolamine to adjust the pH to 6.8-7.2, stir well, and add 1 drop of ethyl paraben solution to obtain Panlong jade lithospermum gel.

[0036] See also Figure 2 The appearance of the Panlong Black Jade Lithospermum gel prepared in Example 2 is dark purple, and the appearance of the Panlong Green Jade Lithospermum gel is dark purple-red. The gel surface is smooth, and the delicate feel, viscosity, and ductility are all good. The centrifugal stability and 4°C stability are good, and the pH is about 7.

[0037] Example 3 Take 1 g of glycerol and add 0.05 g of carbomer 940 to grind finely, add 10 mL of distilled water to swell it, add 1 g of lithospermum oil and 0.4 g of Tween 80 to the above mixture, add 0.1 g of Panlong black jade nanopowder, add triethanolamine to adjust the pH to 6.8-7.2, stir evenly, and add 1 drop of ethyl paraben solution to obtain Panlong black jade lithospermum gel.

[0038] See also Figure 3 The Panlong Black Jade Lithospermum Gel prepared in Example 3 has a dark purple appearance, a smooth surface, a fine feel, and good viscosity, good centrifugal stability, and 24h stability (4°C), and a pH of about 7.

[0039] Comparative Example 1 Take 1 g of glycerol and add 0.05 g of carbomer 940 to grind finely, add 10 mL of distilled water to swell it, add 1 / 0.25 / 0.1 / 0.05 / 0 g of lithospermum oil, and use peanut oil to make up if the oil phase is less than 1 g. Add 0.4 g of Tween 80 to the above mixture, add triethanolamine to adjust the pH to 6.8-7.2, stir evenly, and add 1 drop of ethyl paraben solution to obtain lithospermum gel with different proportions of lithospermum oil and peanut oil in the lithospermum oil mixed oil phase.

[0040] See also Figure 4 The lithospermum gel prepared in comparative example 1 presents different colors according to the different proportions of lithospermum oil and peanut oil in the lithospermum oil mixed oil phase. The prepared gel has a smooth surface, fine feel, good viscosity and ductility, good centrifugal stability and 4°C stability, and a pH of about 7.

[0041] Comparative Example 2 Take 1 g of glycerol and add 0.1 g of carbomer 940 to grind finely, add 10 mL of distilled water to swell it, add 1 g of lithospermum oil and 0.2 g of Tween 80 to the above mixture, add 1 g of Panlong black jade powder (100 mesh), add triethanolamine to adjust the pH to 6.8-7.2, stir well, and add 1 drop of ethyl paraben solution to obtain Panlong black jade lithospermum gel.

[0042] See also Figure 5 The color of the Panlong Black Jade Lithospermum gel prepared in Comparative Example 2 with excessive addition of Panlong Black Jade powder is close to black, the surface of the gel is matte, rough and sticky, not easy to apply, and stratification occurs during centrifugation.

[0043] Comparative Example 3 Take 1 g of glycerol and add 0.1 g of carbomer 940 to grind finely. Add 10 mL of distilled water to swell it, add 1 g of peanut oil and 0.2 g of Tween 80 to the above mixture, add 0.01 / 0.02 / 0.04 g of Panlong black jade nanopowder or 0.2 / 0.5 g of Panlong green jade nanopowder, add triethanolamine, adjust the pH to 6.8-7.2, stir well, and add 1 drop of ethylparaben solution to obtain Panlong black jade gel and Panlong green jade gel.

[0044] See also Figure 6 The Panlong green jade gel and Panlong black jade gel prepared in comparative example 3 show different colors according to the amount of Panlong green jade powder and Panlong black jade powder added. The prepared gel has a smooth surface, good fineness, viscosity and ductility, good centrifugal stability and 4°C stability, and a pH of about 7.

[0045] Example 4 Determination of the content of shikonin, a key component in lithospermum officinale oil and Panlong black jade lithospermum officinale gel Chromatographic conditions: Elite C18 column (4.6 mm x 250 mm); mobile phase: methanol-0.025 mol / L phosphoric acid (85:15); flow rate: 1.0 mL / min; detection wavelength: 516 nm; column temperature: 25°C; injection volume: 5 μL.

[0046] Preparation of reference solution: Accurately weigh 10.0 mg of shikonin reference substance into a 10 mL volumetric flask, dissolve it in methanol and dilute to the mark to make a 1 mg / mL solution as the reference stock solution. Then add methanol to dilute to 0.5, 1, 2, 5, 10, 25, 50, 100, and 200 μg / mL respectively.

[0047] Preparation of the test solution: a. Accurately pipette 0.1 mL of lithospermum oil sample, add 1 mL of methanol, vortex for 15 minutes, ultrasonically extract for 15 minutes, and centrifuge at 14,000 rpm for 10 minutes. Transfer the supernatant to a new EP tube, add 1 mL of methanol to the lower oil phase, repeat the above extraction process, combine the two supernatants, dilute to 2 mL, and centrifuge at 14,000 rpm for 20 minutes. The supernatant is used as the test solution - lithospermum oil. b. Accurately weigh 0.5 g of Panlong Black Jade Lithospermum Gel sample, add 1 mL of methanol, vortex for 15 minutes, ultrasonically extract for 15 minutes, and centrifuge at 14,000 rpm for 10 minutes. Transfer the supernatant to a new EP tube, add 1 mL of methanol to the lower gel phase, repeat the above extraction process, combine the two supernatants, dilute to 2 mL, and centrifuge at 14,000 rpm for 20 minutes. The supernatant is used as the test solution - Panlong Black Jade Lithospermum Gel.

[0048] Linear relationship investigation: 5 μL of reference solution with concentrations of 0.5, 1, 2, 5, 10, 25, 50, and 100 μg / mL was injected respectively, and the peak area was measured. The concentration (C) was used to perform linear regression on the peak area integral value (A). The regression equation was A=7341.5C+3603.7, R 2 =0.9998, indicating that shikonin has a good linear relationship with the peak area integral value in the concentration range of 0 ~ 100 μg / mL.

[0049] Five samples of lithospermum officinale oil were prepared according to the method of test solution a and determined under the above chromatographic conditions. The average content was 76.30 μg / mL, with an RSD of 2.38% (n=5) (see Figure 1 ).

[0050] Five samples of the prepared Panlong Black Jade Lithospermum gel were taken and prepared according to the method of the test solution b. The shikonin content in the Panlong Black Jade Lithospermum gel was determined according to the above chromatographic conditions. The results showed that the shikonin content in the Panlong Black Jade Lithospermum gel was 5.17±0.83 μg / g.

[0051] Example 5 Study on the anti-inflammatory effect of Panlong Moyu Lithospermum officinale gel in vitro Experimental purpose: To detect the effects of lithospermum officinale oil and Panlong Black Jade Lithospermum officinale gel on the secretion of NO, TNF-α and IL-6 in RAW 264.7 macrophages Cell culture and plating: RAW 264.7 mouse macrophages were cultured in DMEM high glucose medium (containing 10% inactivated FBS and 1% double-antibody) at 37°C in a cell culture incubator with 5% CO2 and passaged. 24-well transwell chambers with a pore size of 3 μm and a membrane area of ​​0.33 cm were used. 2 After moistening the chamber membrane with culture medium, the chamber was inverted with the bottom facing up, and 50 μL of RAW264.7 cell suspension in the logarithmic growth phase (density of 4×10 6 / mL), placed in a cell culture incubator and cultured for 4 hours until the membrane adhered, the chamber was placed upright, 500 μL of culture medium was added to the bottom of the well plate, and cultured for 16 hours.

[0052] Grouping and Dosing: Cells were divided into a normal control group, an LPS group, an LPS + lithospermum oil group, and an LPS + Panlong Black Jade Lithospermum Gel group. 0.05 g of Panlong Black Jade Lithospermum Gel (containing 0.26 μg shikonin and 0.4 mg Panlong Black Jade powder) prepared according to Example 3, 50 μL of a lithospermum oil / DMEM mixture (containing an equal amount of shikonin), or 50 μL of DMEM were added to the upper layer of the transwell chamber. After incubation in a cell culture incubator for 30 min, the lower layer of the culture medium in the other three groups, except the normal control group, was replaced with DMEM supplemented with 1 μg / mL LPS. The lower layer of the normal control group was replaced with normal DMEM. After 24 h, the cell culture medium was collected and centrifuged at 1200 rpm for 10 min. The supernatant was then collected for analysis. Absorbance was measured at 540 nm using a Griess assay kit in strict accordance with the kit instructions, and NO concentration in the cell supernatant was calculated. The absorbance at 450 nm was detected using the corresponding Elisa kit in strict accordance with the kit instructions, and the concentrations of TNF-α and IL-6 in the cell supernatant were calculated.

[0053] Effect on IL-6: Experimental results confirmed that after stimulating RAW 264.7 mouse macrophages with LPS (1 μg / mL), IL-6 levels in the cell supernatant significantly increased (P<0.001). Both lithospermum oil and Panlong Black Jade Lithospermum gel significantly inhibited IL-6 production (P<0.0001, P<0.001). Panlong Black Jade Lithospermum gel was more effective than lithospermum oil in inhibiting IL-6 production (P<0.05). This suggests that Panlong Black Jade Lithospermum gel has a significant anti-inflammatory effect in vitro.

[0054] Effect on TNF-α: The experimental results confirmed that after stimulating RAW 264.7 mouse macrophages with LPS (1 μg / mL), the TNF-α content in the cell supernatant increased significantly (P<0.0001). Both lithospermum oil and Panlong Black Jade Lithospermum gel could significantly inhibit the production of TNF-α (P<0.05, P<0.0001). Compared with lithospermum oil, Panlong Black Jade Lithospermum gel could better inhibit the production of TNF-α (P<0.01), indicating that Panlong Black Jade Lithospermum gel has good anti-inflammatory effect in vitro.

[0055] Effect on NO production: Experimental results confirmed that after stimulating RAW 264.7 mouse macrophages with LPS (1 μg / mL), the NO content in the cell supernatant increased significantly (P<0.05). Both lithospermum oil and Panlong Black Jade Lithospermum Gel could significantly inhibit the production of NO (P<0.01, P<0.01), indicating that Panlong Black Jade Lithospermum Gel has good anti-inflammatory effect in vitro.

[0056] Those skilled in the art should recognize that the above embodiments are merely intended to illustrate the present invention and are not intended to limit the present invention. As long as they are within the spirit of the present invention, any changes or modifications to the above embodiments will fall within the scope of the present invention.

Claims

1. A Panlongyu lithospermum gel, comprising by weight: 20 to 50 parts of comfrey oil mixed oil phase, 1 to 25 parts of Panlong jade powder, 10 to 40 parts of surfactant, 2 to 8 parts of gel base, 200 to 700 parts of aqueous phase, 25 to 75 parts of moisturizer, 1 to 5 parts of pH adjuster and 0.5 to 2 parts of preservative; The lithospermum oil mixed oil phase consists of lithospermum oil and a first oil phase, wherein the mass ratio of the lithospermum oil to the first oil phase is 1:(0-10), and the lithospermum oil is prepared by wetting and impregnating segmented or granular lithospermum with a second oil phase and then percolating.

2. The Panlongyu Lithospermum gel according to claim 1, characterized in that The Panlong jade powder is Panlong green jade powder and / or Panlong black jade powder, and the particle size of the Panlong jade powder is 80 nm~200µm.

3. The Panlongyu Lithospermum gel according to claim 1 or 2, characterized in that The first oil phase and / or the second oil phase is vegetable oil. Preferably, the vegetable oil is at least one of sesame oil, soybean oil, corn oil, olive oil, jojoba oil and peanut oil.

4. The Panlongyu Lithospermum gel according to any one of claims 1 to 3, characterized in that The surfactant is at least one of Tween 80, Span 80, and polyvinyl alcohol (PVA); the gel matrix is ​​at least one of carbomer, methylcellulose, and sodium carboxymethylcellulose; the aqueous phase is a phosphate buffer solution or distilled water; the moisturizer is at least one of glycerol, sodium hyaluronate, trehalose, and hydroxypropyl methylcellulose; the pH adjuster is triethanolamine or sodium hydroxide; and the preservative is at least one of parabens, benzoates, sorbates, and propylene glycol.

5. A method for preparing the Panlongyu Lithospermum officinale gel according to any one of claims 1 to 4, comprising: Cutting lithospermum into sections or crushing them, wetting with a second oil phase, impregnating and then percolating to obtain lithospermum oil; Mixing the lithospermum oil with the first oil phase to obtain the lithospermum oil mixed oil phase; Grinding the gel matrix and moisturizer into fine powder, adding water and mixing evenly to fully swell the gel matrix; The lithospermum oil mixed oil phase obtained in step 2) is mixed evenly with the gel matrix obtained in step 3), and then Panlongyu jade powder is added and mixed evenly. Then, a surfactant, a pH regulator and a preservative are added in sequence and mixed evenly to obtain the Panlongyu lithospermum gel.

6. The preparation method according to claim 5, characterized in that In step 1), the mass ratio of the lithospermum officinale to the second oil phase is 1:(2-5).

7. The preparation method according to claim 5 or 6, characterized in that: In step 1), the wetting and soaking time is 2 to 10 days.

8. Use of the Panlongyu Lithospermum Glyceride Gel according to any one of claims 1 to 4 in the preparation of a medicament for treating burns, scalds, pressure sores, psoriasis, eczema, skin ulcers, and dermatitis.

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