A composition, an article comprising the composition, and an application thereof

Abstract

This invention provides a composition, an article comprising the same, and its application, relating to the field of dairy product technology. The composition provided by this invention comprises, by weight, the following components: 5-8 parts black cohosh extract, 0.5-2.5 parts banana flower extract, and 1.5-2.5 parts rice bran oil extract. In this composition, the black cohosh extract, rice bran oil extract, and banana flower extract work synergistically to maintain a good balance in bone calcium absorption and metabolism, significantly improving bone density, and is particularly applicable to improving bone density in middle-aged and elderly individuals and postmenopausal women.

Description

Technical Field

[0001] This invention relates to the field of dairy technology, and more particularly to a composition, articles comprising the composition, and applications thereof. Background Technology

[0002] Changes in bone density and decreased bone mass increase the risk of fractures in clinical practice. Decreased bone density is commonly seen in middle-aged and elderly individuals, with a significantly higher incidence in postmenopausal women. Bone density is closely related to hormone levels and nutrient absorption capacity. Currently, most methods for increasing bone density in middle-aged and elderly individuals involve dietary adjustments, such as supplementing with calcium-rich milk powder and protein powder, or taking vitamin D and calcium supplements. However, due to individual factors, chewing and digesting calcium tablets remains a significant challenge for adults, often resulting in a mismatch between supplementation, absorption, and utilization. For postmenopausal women with decreased bone density, current treatments primarily involve estrogen, growth hormone, and calcitonin. However, individual differences can lead to adverse reactions and side effects, such as breast tenderness, vaginal bleeding, increased risk of breast and endometrial cancer with long-term use, and the development of drug resistance. Therefore, finding and developing safer products to improve bone density, especially for elderly postmenopausal women, has become a new focus.

[0003] The existing technology CN105454464 discloses a calcium-supplementing and lipid-lowering rice bran oil, which contains a variety of ingredients such as rice bran oil, rapeseed oil, olive oil, beef bone, vitamins, walnut kernels, cassia seed pretreatment, honeysuckle extract, licorice, lysimachia christinae, ginseng and red dates. However, its solution is only to provide as much calcium as possible, and it cannot solve the problem of severe bone calcium loss in the human body and the difficulty in absorbing calcium supplements, thus failing to improve bone density. Summary of the Invention

[0004] This invention addresses the problem that current calcium supplements are ineffective in improving bone density, especially for middle-aged and elderly people and postmenopausal women who suffer from severe bone calcium loss and have difficulty absorbing calcium supplements. It provides a composition that, through the synergistic effect of its components, effectively reduces bone calcium loss, promotes calcium absorption, and thus jointly regulates the body's calcium metabolism balance, thereby increasing bone density and achieving a good effect on improving bone density.

[0005] Another object of the present invention is to provide the use of the composition in the preparation of articles that improve bone density.

[0006] Another object of the present invention is to provide an article of manufacture.

[0007] In a first aspect, the present invention provides a composition comprising, by parts by mass, the following components:

[0008] Black cohosh extract 5-8 parts, banana flower extract 0.5-2.5 parts, rice bran oil extract 1.5-2.5 parts.

[0009] According to a composition provided by the present invention, preferably, the rice bran oil extract contains ≥40wt% oryzanol.

[0010] According to the present invention, a composition preferably further comprises, by weight parts:

[0011] 1-3 parts of frog oil and 1-3 parts of valerian root extract.

[0012] According to a composition provided by the present invention, preferably, the total valeric acid content of the valerian root extract is ≥1 wt%.

[0013] According to a composition provided by the present invention, preferably, the protein content of the frog oil is ≥50wt%.

[0014] In a second aspect, the present invention also provides the use of the composition in the preparation of articles that improve bone density.

[0015] The use of a composition provided by the present invention in the preparation of articles for improving bone density, preferably the articles for improving bone density are for middle-aged and elderly people and / or postmenopausal women.

[0016] Thirdly, the present invention also provides an article comprising the composition.

[0017] According to the present invention, an article for improving bone density is preferably a dairy product, and more preferably, the dairy product includes the composition, milk powder and other excipients.

[0018] According to the present invention, preferably, the dairy product comprises, by weight, the following components:

[0019] The composition consists of 7-19 parts of carbohydrates, 4-8 parts of sugars, 1-4 parts of dietary fiber, 3-6 parts of grains, and 10-20 parts of milk powder.

[0020] According to a product provided by the present invention, preferably, the mass ratio of black cohosh extract, banana flower extract and rice bran oil extract in the product is 7:1:2.

[0021] According to a product provided by the present invention, preferably, the mass ratio of frog oil and valerian root extract in the product is 5:3.

[0022] Beneficial effects:

[0023] This invention provides a composition in which black cohosh extract mainly contains triterpenoid saponins that can adjust estrogen receptors and inhibit osteoclast activity, thereby reducing bone loss; rice bran oil extract contains oryzanol, which can promote calcium absorption and increase overall antioxidant capacity, thereby maintaining the balance of bone metabolism in the body; and banana flower extract mainly contains polyphenols and potassium and magnesium elements, which can regulate calcium metabolism. The three components work synergistically to maintain a good balance of bone calcium absorption and metabolism, significantly increasing bone density values, and have a good effect on increasing bone density, especially applicable to middle-aged and elderly people and postmenopausal women to increase bone density. Detailed Implementation

[0024] The following examples are for illustrative purposes only and are not intended to limit the scope of the invention. Where specific techniques or conditions are not specified in the examples, they should be performed according to the techniques or conditions described in the literature in this field, or according to the product instructions. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased from legitimate channels.

[0025] The main sources of raw materials are explained below:

[0026] Forest frog oil (purchased from Changbai Mountain Ecological Food Co., Ltd.): Protein content 51wt%. Before use, clean, dry, grind, and pass through a 200-mesh sieve.

[0027] Black cohosh extract (purchased from Shaanxi Huike Botanical Co., Ltd.): water extraction ratio 10:1 (10 kg of raw material concentrated into 1 kg of extract powder); raw material is in powder form, particle size ≥ 80 mesh, moisture content ≤ 6 wt%;

[0028] Banana flower extract (purchased from Shaanxi Sinote Biotechnology Co., Ltd.): alcohol extraction 10:1 (10 kg of raw material concentrated into 1 kg of extract powder), the raw material is in powder form, particle size ≥80 mesh, moisture content ≤6wt%;

[0029] Rice bran oil powder extract (purchased from Shaanxi Bolin Biotechnology Co., Ltd.): oryzanol content 40wt%;

[0030] Valerian root extract (imported from Italy): total valeric acid content 1wt%.

[0031] Job's tears: Agricultural products purchased from the market.

[0032] Unless otherwise specified, the raw materials mentioned in the embodiments and comparative examples of this invention are all the same raw materials.

[0033] In a specific embodiment, the present invention provides a composition comprising, by weight parts, the following components:

[0034] Black cohosh extract 5-8 parts, banana flower extract 0.5-2.5 parts, rice bran oil extract 1.5-2.5 parts.

[0035] Bone metabolism imbalance leads to two main issues: firstly, bone loss and damage to bone microstructure, manifested in increased osteoclast activity. Osteoclasts mediate enhanced bone resorption, thus promoting bone loss. In perimenopausal women, estrogen regulation further enhances osteoclast activity, resulting in bone resorption exceeding bone formation. Secondly, decreased osteoblast function leads to reduced bone matrix synthesis and mineralization. Inflammatory responses also inhibit osteoblast differentiation, all contributing to decreased bone density. Triterpenoid saponins in black cohosh extract can regulate bone metabolism, inhibit osteoclast activity, and modulate the inflammatory environment. Specifically, they inhibit the binding of nuclear factor κB receptor activator ligand (RANKL) to its receptor RANK, reducing osteoclast differentiation. Polyphenols in banana flower extract act on metabolic pathways, upregulating osteoprotegerin (OPG) expression, increasing the OPG / RANKL ratio, and inhibiting osteoclast activation. These polyphenols work synergistically with black cohosh extract to regulate osteoclast activity. Furthermore, plant polysaccharides in banana flower extract promote microbial metabolism, increase short-chain fatty acids in the gut, and promote osteogenic differentiation. Oryzanol in rice bran oil extract can block NF-κB inflammatory signals and inhibit osteoclast activity. It works synergistically with banana flower extract to inhibit intestinal cholesterol absorption and reduce blood cholesterol levels, thereby reducing the release of inflammatory factors and slowing down bone loss. Oryzanol has estrogen-like activity in its structure and can indirectly alleviate the effects of rapid estrogen loss on bone metabolism during perimenopause.

[0036] In the composition of this invention, black cohosh extract mainly contains triterpenoid saponins, which can adjust estrogen receptors to inhibit osteoclast activity and thus reduce bone loss; rice bran oil extract contains oryzanol, which can promote calcium absorption and increase overall antioxidant capacity, thereby maintaining the balance of bone metabolism in the body; banana flower extract mainly contains polyphenols and potassium and magnesium elements, which can regulate calcium metabolism. The three work synergistically to maintain a good balance of bone calcium absorption and metabolism, significantly improve bone density, and have a good effect on increasing bone density.

[0037] In some specific embodiments, the black cohosh extract of the present invention may be a point value of 5 parts, 6 parts, 7 parts, 8 parts, or any range of arbitrary values; the banana flower extract may be a point value of 0.5 parts, 1.0 parts, 1.5 parts, 2.0 parts, 2.5 parts, or any range of arbitrary values; and the rice bran oil extract may be a point value of 1.5 parts, 1.8 parts, 2.0 parts, 2.5 parts, or any range of arbitrary values.

[0038] In some specific embodiments, in order to achieve a better bone density increase effect, the present invention may further preferably contain ≥40wt% oryzanol in the rice bran oil extract.

[0039] Oryzanol is an active ingredient in rice bran oil extract. Increasing the oryzanol content in rice bran oil extract can, to a certain extent, help to better exert its efficacy and reduce the amount of rice bran oil extract used in the composition. However, the relationship between the oryzanol content in rice bran oil extract and the overall bone density increase effect of the composition of this invention is not linear. Oryzanol is a mixture of ferulic acid esters, mainly composed of triterpenoid alcohols. In bone metabolism, it mainly enhances the activity of antioxidant enzymes by activating the Nrf2 pathway. The enzyme content and carrier protein content in the body are also fixed. When the enzyme metabolism reaches its maximum capacity, increasing the enzyme metabolism activity cannot continuously increase the enzyme metabolism capacity. Therefore, the upper limit of the oryzanol content can be determined based on the actual effect.

[0040] Studies of the compositions provided in this invention have also revealed that for those with decreased bone density, especially middle-aged and elderly individuals and postmenopausal women, not only is bone calcium loss severe, but sleep quality is also generally poor, resulting in poor calcium absorption and utilization efficiency during calcium supplementation. Furthermore, in some specific embodiments, the compositions provided in this invention are further preferred to include, by weight parts, the following components: 1-3 parts of frog oil and 1-3 parts of valerian root extract.

[0041] The addition of specific amounts of frog oil and valerian root extract can further improve sleep quality. On one hand, it works synergistically with the black cohosh extract, banana flower extract, and rice bran oil extract of this invention to increase the absorption and conversion rate of bone calcium in the body, thus better regulating bone calcium metabolism and achieving a better bone calcium balance. On the other hand, it can also prolong the effective sleep time for calcium absorption, extending the absorption and conversion time of calcium in the body, which is more conducive to the full absorption and conversion of effective calcium already in the body system, thereby increasing the amount of calcium absorbed and converted, and further contributing to a better bone calcium balance.

[0042] The above-mentioned effects can make the preferred solution provided by the present invention more conducive to improving bone density and effectively reducing bone calcium loss.

[0043] In some specific embodiments, in order to ensure the full manifestation of the effects of the active ingredients and achieve better bone density improvement, the present invention further preferably has a total valeric acid content of ≥1wt% in the valerian root extract.

[0044] In some specific embodiments, in order to better supplement protein, enhance overall bodily functions, and better promote calcium absorption and conversion rate while promoting sleep improvement, the present invention further preferably has a protein content of ≥50wt% in the frog oil.

[0045] In other specific embodiments, the present invention also provides the use of the composition in the preparation of articles that improve bone density.

[0046] In some specific embodiments, the bone density improvement products provided by the present invention are products for improving bone density in middle-aged and elderly people and / or postmenopausal women.

[0047] Perimenopause women are affected by estrogen regulation, which increases osteoclast activity, resulting in bone resorption exceeding bone formation. In middle-aged and elderly people, osteoblast function decreases, making them more prone to bone calcium loss. Therefore, the composition of this invention, which is designed to improve bone calcium balance, is more effective in preparing products that improve bone density.

[0048] In some specific embodiments, the present invention also provides an article of manufacture comprising the aforementioned composition.

[0049] The products of this invention may include food products and functional products, and food products may include dairy products, beverages, and especially dairy-containing foods.

[0050] In other specific embodiments, in order to provide both high protein and high nutrition, the product of the present invention is preferably a dairy product, including the composition, milk powder and other excipients provided above by the present invention.

[0051] In specific embodiments, this invention does not specifically limit the types of dairy products; any dairy product that can be added to the composition provided by this invention, as determined by those skilled in the art, is included within the scope of the dairy products mentioned. The use of other excipients can be added according to the actual production and preparation requirements of the dairy product.

[0052] In some specific embodiments, the present invention also provides a dairy product comprising, by weight, the following components:

[0053] The composition consists of 7-19 parts of carbohydrates, 4-8 parts of sugars, 1-4 parts of dietary fiber, 3-6 parts of grains, and 10-20 parts of milk powder.

[0054] The sugars mentioned in this invention include trehalose, sucrose and its derivatives, syrups, maltose, etc.

[0055] The dietary fiber mentioned in this invention includes fruit powders, preferably fruit powders with high vitamin C content, such as acerola cherry powder, prickly pear powder, sea buckthorn powder, etc.

[0056] The grains mentioned in this invention include Job's tears, rice, millet, wheat, red rice, black rice, corn, barley, oats, etc.

[0057] In a specific implementation, the total amount of the above-mentioned dairy products is calculated in parts by weight of 100, with the remainder being replenished by production water.

[0058] Specific products, such as dairy products, can be composed of the following two categories:

[0059] Category I dairy products, by weight, include the following components: 5-8 parts black cohosh extract, 0.5-2.5 parts banana flower extract, 1.5-2.5 parts rice bran oil extract, 4-8 parts trehalose, 1-4 parts fruit powder, 3-6 parts coix seed, 10-20 parts milk powder, and the remaining part is made up to 100 parts by production water.

[0060] The second category of dairy products, by weight, includes the following components: 5-8 parts black cohosh extract, 0.5-2.5 parts banana flower extract, 1.5-2.5 parts rice bran oil extract, 1-3 parts frog oil, 1-3 parts valerian root extract, 4-8 parts trehalose, 1-4 parts fruit powder, 3-6 parts coix seed, 10-20 parts milk powder, and the remaining part is made up to 100 parts by production water.

[0061] In some specific embodiments, in order to obtain better sensory evaluation results and comprehensive improvement in bone density, the present invention preferably uses a mass ratio of black cohosh extract, banana flower extract and rice bran oil extract of 7:1:2 in the product.

[0062] In some specific embodiments, in order to obtain better sensory evaluation results and comprehensive improvement in bone density, the present invention further preferably uses a mass ratio of frog oil and valerian root extract of 5:3 in the product.

[0063] In a specific implementation, in order to make it easier for middle-aged and elderly patients to use, and to solve the problem of adult calcium tablets being difficult to chew and digest and absorb while promoting the balance of bone calcium absorption and metabolism, the product of the present invention is preferably a dairy product, which can be prepared into the form of milk porridge, making it convenient to eat and absorb.

[0064] In some embodiments, the present invention also specifically provides a method for preparing milk porridge, comprising the following steps:

[0065] Soaking: Add the crushed and sieved frog oil powder to room temperature water, stir at 45~90 rpm, and allow it to fully swell for 4~6 hours. Then, shear the liquid at high shear (6000 rpm) for 15~25 minutes and filter it through a 200-mesh filter bag to obtain the filter residue as material A.

[0066] Soaking: Steam the Job's tears for 7-10 minutes, then put them in a mesh bag and soak them in room temperature water for 6 hours to make material B.

[0067] Preparation: Add material A and material B to water at 50~60℃, slowly add the remaining components, heat to 80~90℃ and cook for 15~25 minutes to obtain milk porridge sample.

[0068] Filling: Fill the sample according to the packaging specifications;

[0069] Sterilization: 85℃, 25~40min spray sterilization for filling samples.

[0070] Examples 1-9

[0071] A composition comprising, by weight, the components shown in Table 1 below.

[0072] This embodiment also provides a dairy product for improving bone density – milk porridge, which includes the components shown in Table 1 below by mass.

[0073] In Example 5, the added sleep-aiding ingredient is GABA (γ-aminobutyric acid), a common and frequently used sleep-promoting substance. The amount added is 4 parts, replacing the sleep-aiding ingredients, frog oil and valerian root extract, in Example 3. (The actual dosage of GABA is 500 mg / day (refer to GB 2760-2014), the remainder being product excipients.)

[0074] This embodiment also provides a method for preparing a dairy product—milk porridge—that improves bone density, as follows:

[0075] Soaking: Add the crushed and sieved frog oil powder to room temperature water, stir at 60 rpm, and allow it to fully swell for 5 hours. Then, shear the liquid at high shear (6000 rpm) for 20 minutes, pass it through a 200-mesh filter bag, and keep the filter residue as material A.

[0076] Soaking: Steam the Job's tears in a steamer for 8 minutes, then put them in a mesh bag and soak them in room temperature water for 6 hours to make material B.

[0077] Preparation: Add material A and material B to water at 55°C, slowly add the remaining components, heat to 90°C and cook for 20 minutes to obtain a milk porridge sample.

[0078] Filling: Fill the sample according to the packaging specifications;

[0079] Sterilization: 85℃, 30min spray sterilization for filling samples.

[0080] If the relevant ingredients are not available, the corresponding steps can be omitted.

[0081] Table 1. Component content of each example

[0082]

[0083] Comparative Examples 1-6

[0084] A milk porridge, by mass parts, comprises the components shown in Table 2 below.

[0085] The milk porridge was prepared according to the method for preparing dairy products-milk porridge provided in Example 1, as follows:

[0086] Soaking: Add the crushed and sieved frog oil powder to room temperature water, stir at 60 rpm, and allow it to fully swell for 5 hours. Then, shear the liquid at high shear (6000 rpm) for 20 minutes, pass it through a 200-mesh filter bag, and keep the filter residue as A.

[0087] Soaking: Steam the Job's tears in a steamer for 8 minutes, then put them in a mesh bag and soak them in room temperature water for 6 hours to make B.

[0088] Preparation: Add A and B to water at 55°C, slowly add the remaining components, heat to 90°C and cook for 20 minutes to obtain a milk porridge sample.

[0089] Filling: Fill the sample according to the packaging specifications;

[0090] Sterilization: 85℃, 30min spray sterilization for filling samples.

[0091] If the relevant ingredients are not available, the corresponding steps can be omitted.

[0092] Table 2. Content of each component in the comparative example

[0093]

[0094] Result detection

[0095] The milk porridge prepared in the above embodiments and comparative examples was subjected to relevant performance tests.

[0096] The detection method is as follows:

[0097] The samples prepared in Examples 1-4 were subjected to sensory evaluation. A total of 50 people participated in the sensory evaluation, and the scoring criteria are shown in Table 3 below.

[0098] Table 3. Sensory Evaluation Criteria

[0099]

[0100] The sensory evaluation results are shown in Table 4.

[0101] Table 4. Sensory evaluation results of Examples 1-9

[0102]

[0103] The sensory evaluation was divided into four dimensions, but the final evaluation was based on the total score, as shown in Table 4 above.

[0104] Black cohosh extract has a slightly bitter herbal taste. When heated at high temperatures, the bitterness increases as saponins hydrolyze, and an unpleasant cooked taste is produced. Banana flower extract contains polyphenols, but prolonged high temperatures can cause a burnt, bitter taste, and the degradation of esters can produce an astringent feeling. Banana flower extract can also cause the overall product color to be darker. Rice bran oil extract is an oily substance, and its stability and flavor are easily oxidized at high temperatures. When heated, it can undergo a Maillard reaction with milk powder at high temperatures, producing brown products and bitterness, which can change the product color and deteriorate its flavor.

[0105] The most prominent flavor of frog oil is its fishy smell. When heated at high temperatures, it will produce a fishy and oily smell, and its texture will become sticky. If heated for too long, it will develop a rancid and bitter taste.

[0106] Example 3 represents the optimal component ratio of the present invention. Through the synergistic effect of each component in the milk porridge, it not only ensures the bone calcium balance effect, but also scores high in flavor, color, taste and texture, resulting in the best overall sensory evaluation and ensuring the overall flavor and taste of the milk porridge.

[0107] In Examples 1 and 2, the banana flower extract content was higher than that in Example 3, resulting in a darker overall product color. At the same time, the flavor of the valerian root extract was affected by heat processing, resulting in an unpleasant cooked taste.

[0108] Examples 5 and 6 did not include frog oil and valerian root extract, two ingredients that have a significant impact on flavor. The overall products showed significant improvement in rancidity and cooked flavor.

[0109] Example 7: When heated, frog oil produces a fishy and oily smell, and the overall taste of the product becomes sticky. At the same time, due to the increase in content, it will produce a bitter taste.

[0110] In Example 8, as the amount of valerian root extract added increased, a sour and putrid odor was produced during heat processing, and the earthy smell of the herbaceous plant intensified, with a noticeable burnt and bitter taste.

[0111] As can be seen from the data in Table 4 above, the overall sensory evaluation of the milk porridge samples provided by this invention can reach 68 points or above, which does not affect the edibility of the milk porridge samples.

[0112] Bone density test:

[0113] Thirty postmenopausal women aged 55-65 years without bone metabolic diseases were selected. They had not taken any bone-improving products in the three months prior to the test. The experimental test participants took the milk porridge product provided in the example for a fixed period of 60 days, once a day, 200g each time. During the period of taking the product, no other bone-improving drugs or foods were added.

[0114] Referring to the 2017 Guidelines for the Diagnosis and Treatment of Primary Osteoporosis, dual-energy X-ray absorptiometry (DEXA) was used to measure the bone mineral density (BMD) and T values ​​of the lumbar spine (L1-L4, overall) and the left humeral neck.

[0115] The measurement results are shown in Table 5 below.

[0116] Table 5. Bone mineral density measurement results of the examples and comparative examples

[0117]

[0118] Black cohosh extract mainly contains triterpenoid saponins, which can adjust estrogen receptors and inhibit osteoclast activity, thereby reducing bone loss. Rice bran oil extract can promote calcium absorption and increase overall antioxidant capacity, thereby maintaining the balance of bone metabolism in the body. Banana flower extract mainly contains polyphenols and potassium and magnesium elements, which can regulate calcium metabolism.

[0119] Example 3 shows that before and after taking the product, the test subject's bone density improved by 0.7, indicating a good effect on balancing bone calcium absorption.

[0120] Example 5 shows that after replacing the sleep-aiding ingredients (frog oil and valerian root extract) of the present invention with the commonly used sleep-aiding ingredient GABA (γ-aminobutyric acid), the change in bone density of the test subjects was only 0.35, far lower than that in Example 3. This indicates that the synergistic effect of the overall formula of the present invention is significantly better than that of conventional sleep-aiding ingredients in improving bone calcium balance. Example 6, without the addition of frog oil and valerian root extract, which have sleep-aiding effects, did not show an increase in effective sleep time before and after administration, and the change in bone density before and after administration was also significantly lower than that in Example 3. Example 7 added frog oil, and Example 8 added valerian root extract, which had a certain improvement effect on effective sleep, but it was still not as good as the synergistic effect of Examples 1-4. In Example 9, the proportion of valerian root extract and frog oil added was not within the preferred range, and the bone density improvement effect was also not as good as that of Examples 1-4.

[0121] Comparative Example 1, without the addition of black cohosh extract, showed a change in improvement of 0.15 after administration. Comparative Example 2, without the addition of banana flower extract, showed a change in improvement of 0.16 after administration. Comparative Example 3, without the addition of rice bran oil extract, showed a change in improvement of 0.12 after administration. The synergistic formulation of key components in Comparative Examples 4 and 5 is not within the scope of this invention; the overall bone calcium balance effect was significantly weakened, and the changes in improvement after administration were only 0.18 and 0.2, respectively.

[0122] Meanwhile, the total mass fractions of black cohosh extract, banana flower extract, and rice bran oil extract used in Comparative Examples 1-3 were consistent with those in Example 3, indicating that the components of the composition provided by the present invention have an unexpected synergistic promoting effect, and the effect of the present invention cannot be achieved by randomly selecting and adding them.

[0123] Comparative Example 6 replaced all the active ingredients with vitamin D. The improvement value before and after the test subjects took the supplement was 0.1. Supplementing with vitamin D can improve bone density, but the increase in bone density is slow.

[0124] Sleep-promoting effect test:

[0125] Thirty postmenopausal women aged 55-65 years with no bone metabolic diseases were selected. They had not taken any bone-improving products in the three months prior to the test. The experimental test participants took the milk porridge products provided in Examples 1-9 for a fixed period of 60 days, once a day, 200g each time. No other bone-improving drugs or foods were added during the period of taking the product.

[0126] Record total sleep time: From the moment you decide to fall asleep until you are fully awake, this is considered one sleep cycle. Record the effective sleep time value of one sleep cycle.

[0127] The sleep test results are shown in Table 6 below:

[0128] Table 6. Sleep tests in Examples 1-9

[0129]

[0130] Valerian root extract primarily modulates neurotransmitters to inhibit central nervous system excitability, significantly improving sleep quality and shortening sleep onset time. It also synergistically adjusts estrogen levels in the body with frog oil, thereby improving sleep quality. Example 3 showed a significant increase in sleep time, extending it by 2 hours. Valerian root extract improves sleep quality, and the increased sleep time stimulates osteoblast proliferation and enhances bone formation. The antioxidant and anti-inflammatory properties of frog oil can reduce the accumulation of inflammatory factors during sleep and inhibit bone mineralization. The positive impact of improved sleep on bone calcium absorption balance is consistent with the bone density changes shown in Table 5, which to some extent confirms that the milk porridge product of this invention improves bone density by improving sleep and extending the effective sleep time for calcium absorption.

[0131] The milk porridge samples in Examples 1-4 all had a positive effect on sleep, improving it, but the effect was most significant in the formulation of Example 3. Example 5, using GABA compared to frog oil and valerian root extract, prolonged sleep time by 1 hour, but was not as effective as Example 3.

[0132] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition, characterized in that, Based on parts by mass, it includes the following components: Black cohosh extract 5-8 parts, banana flower extract 0.5-2.5 parts, rice bran oil extract 1.5-2.5 parts.

2. The composition according to claim 1, characterized in that, The rice bran oil extract contains ≥40wt% oryzanol.

3. The composition according to claim 1 or 2, characterized in that, It also includes the following components by parts by mass: 1-3 parts of frog oil and 1-3 parts of valerian root extract.

4. The composition according to claim 3, characterized in that, The total valeric acid content of the valerian root extract is ≥1 wt%, and / or the protein content of the forest frog oil is ≥50 wt%.

5. Use of the composition according to any one of claims 1 to 4 in the preparation of articles for improving bone density.

6. The application according to claim 5, characterized in that, The product for improving bone density is designed for middle-aged and elderly people.

7. The application according to claim 5, characterized in that, The product for improving bone density is for postmenopausal women.

8. An article, characterized in that, Includes the composition according to any one of claims 1 to 4.

9. The article according to claim 8, characterized in that, The products include dairy products.

10. The article of claim 9, characterized in that, The dairy product, by weight, comprises the following components: The composition according to any one of claims 1 to 4 comprises 7 to 19 parts of carbohydrates, 4 to 8 parts of dietary fiber, 1 to 4 parts of cereals, and 10 to 20 parts of milk powder.

11. The article according to any one of claims 8 to 10, characterized in that, The article must meet at least one of the following conditions: (1) The mass ratio of black cohosh extract, banana flower extract and rice bran oil extract in the product is 7:1:2; (2) The mass ratio of frog oil and valerian root extract in the product is 5:3.

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