Industrial production method of black fungus through liquid submerged fermentation

Abstract

The invention relates to the technical field of edible fungus culture medium optimization technology, in particular to a black fungus liquid submerged fermentation industrial production method which comprises the following steps: firstly, performing nano enzymolysis on corn flour, performing ultrasonic alkali treatment on soybean meal, and adding a selenium-germanium-containing synergist and the like when a culture medium is prepared; performing specific sterilization, inoculating space mutagenesis and oxygen-enriched domestication strains, regulating and controlling fermentation in an intelligent fermentation tank according to parameters, and then separating and extracting a product through an advanced technology. The method has obvious advantages; the pretreated raw materials and the synergist promote growth; the fermentation is accurately controlled to guarantee uniform quality, pesticides are reduced, the period is shortened, the cost is reduced, and the innovative technology promotes the industry to develop to high-end intelligence.

Description

Technical Field

[0001] The invention relates to the technical field of edible fungus cultivation matrix optimization technology, in particular to an industrial production method of black fungus liquid submerged fermentation. Background Art

[0002] Black fungus, an edible fungus with a rich nutritional profile and unique flavor, holds a significant position in the global food market. Traditional cultivation of black fungus relies on solid cultivation methods, such as log cultivation and bag cultivation. While these methods have a long history, they suffer from numerous drawbacks and are unable to meet growing market demand.

[0003] Solid-state cultivation is significantly influenced by environmental factors. Even slight fluctuations in temperature, humidity, and light conditions can significantly impact the growth rate and ultimate yield of black fungus. For example, in cooler seasons, black fungus grows slowly, prolonging the production cycle and limiting yield per unit time. Furthermore, solid-state cultivation suffers from low spatial efficiency, making large-scale, high-density cultivation difficult to achieve, severely restricting yield increases.

[0004] Solid cultivation makes it difficult to precisely control the nutrients required for black fungus growth. Different batches of logs or bagged materials can have varying nutritional profiles, leading to inconsistent quality. This not only impacts the product's market competitiveness but also increases the difficulty of ensuring quality control. Furthermore, traditional cultivation methods are susceptible to pests and diseases. To combat these, growers often need to use large amounts of pesticides, which not only increases production costs but can also lead to excessive pesticide residues, posing a health risk to consumers.

[0005] With the acceleration of industrialization, submerged fermentation technology has gradually been introduced into the field of black fungus production. However, existing black fungus submerged fermentation technology still has many problems. Common culture medium formulas are relatively simple and have limited nutrients, which cannot fully meet the nutritional needs of black fungus during the fermentation process. For example, the carbon and nitrogen sources in traditional culture media are single and have low utilization rates, resulting in low fermentation efficiency and insufficient black fungus biomass accumulation.

[0006] Existing technologies often rely on fixed fermentation parameters, unable to adjust in real time based on the microbial growth status and metabolic needs during the fermentation process. This makes it difficult to maintain optimal fermentation conditions, leading to issues such as long fermentation cycles and unstable product quality. Furthermore, traditional separation and extraction processes are relatively crude, resulting in low extraction rates and a low product purity, which reduces the added value of black fungus products.

[0007] The current industrial production of black fungus (Fungus auricularia auricula) using submerged fermentation lacks innovative technologies and ingredient applications. For example, the introduction and utilization of new bioactive substances is limited, making it difficult to improve the quality and yield of black fungus through bioactivity regulation. Furthermore, issues such as low intelligence levels and poor interoperability between production equipment and process integration limit the efficiency and scale of industrial production.

[0008] In summary, developing an innovative and efficient industrial production method for black fungus liquid submerged fermentation is of great significance for solving the shortcomings of existing technologies and promoting the sustainable development of the black fungus industry. Summary of the Invention

[0009] (1) Technical problems solved

[0010] In view of the shortcomings of the existing technology, the present invention provides an industrial production method of black fungus by deep liquid fermentation.

[0011] (2) Technical solution

[0012] An innovative industrial production method for submerged fermentation of black fungus comprises preparing a culture medium by taking, by weight, 22-28 parts of corn flour pretreated with nanoenzymatic hydrolysis, wherein a nanocellulase and amylase composite system is used during the pretreatment process; and 18-22 parts of soybean meal powder treated with ultrasound-assisted alkali. The reaction formula during the treatment is:

[0013]

[0014] 6-8 parts of glucose, 4-6 parts of yeast extract, 0.8-1.5 parts of potassium dihydrogen phosphate, 0.4-0.8 parts of magnesium sulfate, 0.08-0.15 parts of zinc sulfate, and 0.02-0.04 parts of vitamin B1 are mixed; at the same time, a new bioactive synergist Y is added, which is an organic high molecular polymer containing selenium and germanium, and has the chemical formula:

[0015]

[0016] Wherein R and R' are organic groups, and the addition amount is 0.02-0.06 parts; add appropriate amount of water and adjust the pH to 5.7-6.3;

[0017] The subsequent sterilization is carried out by high-pressure sterilization at 123-125°C for 25-30 minutes; after cooling to 26-28°C, black fungus strains screened by space mutagenesis and domesticated in an oxygen-rich environment are inoculated at a volume ratio of 6%-8%; fermentation is carried out in a fermenter with an intelligent feedback control system at a temperature of 24-26°C, a stirring speed of 180-220 r / min, a ventilation volume of 0.8-1.2 vvm, online monitoring of dissolved oxygen, pH, and biomass, and intelligent control of a feed containing a specially formulated carbon and nitrogen source. The carbon source is a mixture of xylose, glucose, and sucrose in a mass ratio of 3:2:1, and the nitrogen source is a mixture of peptone, yeast extract powder, and ammonium sulfate in a mass ratio of 2:1:1. The dissolved oxygen is maintained at 40%-45%, the pH is 6.0-6.1, and the fermentation is carried out for 6-7 days.

[0018] After fermentation, the product is separated by a high-efficiency cross-flow membrane separation device, and the mycelium is treated by freeze-drying combined with supercritical carbon dioxide extraction technology, first freeze-dried at -40-30℃, and then extracted under supercritical carbon dioxide pressure of 10-15MPa and temperature of 35-40℃; the fermentation supernatant is extracted by two-phase aqueous extraction combined with chromatographic separation technology to extract the target components, and subsequent chromatographic separation uses gel permeation chromatography column.

[0019] Preferably, when pretreating corn flour with nanoenzymatic hydrolysis, the composite ratio of nanocellulase and amylase is 3:2-4:1, the enzymatic hydrolysis temperature is 45-50°C, and the time is 2-3 hours.

[0020] Preferably, when ultrasonically assisted alkali treatment of soybean meal powder is performed, the ultrasonic power is 100-150 W, the alkali concentration is 0.5%-1%, and the treatment time is 1-1.5 hours.

[0021] Preferably, the black fungus strains screened by space mutagenesis and domesticated in an oxygen-rich environment are domesticated in an oxygen-rich environment for 5-7 generations, and the strain activity is increased by 30%-40%.

[0022] Preferably, the intelligent feedback control system monitors the fermentation parameters in real time through sensors, collects data every 10-15 minutes, and adjusts the feed rate and ventilation volume according to a preset algorithm.

[0023] Preferably, the membrane pore size of the high-efficiency cross-flow membrane separation device is 0.1-0.2 μm, the operating pressure is 0.1-0.3 MPa, and the cross-flow velocity is 1-2 m / s.

[0024] Preferably, when freeze drying is combined with supercritical carbon dioxide extraction technology to treat the mycelium, the freeze drying time is 8-10 hours and the supercritical carbon dioxide extraction time is 1-2 hours.

[0025] Preferably, when the fermentation supernatant is extracted by aqueous two-phase extraction, the molecular weight of polyethylene glycol is 4000-6000, the molecular weight of dextran is 10000-15000, and the mass ratio of the two is 1:1-1.5:1.

[0026] Preferably, the gel permeation chromatography column has a length of 50-80 cm, an inner diameter of 1-2 cm, an eluent of 0.1 mol / L phosphate buffer, and a flow rate of 0.5-1 mL / min.

[0027] Preferably, the purity of the final products, the black fungus mycelium extract and the polysaccharide extract, are both not less than 95%.

[0028] (3) Beneficial technical effects

[0029] Compared with the existing technology, the beneficial effects of the present invention are:

[0030] 1. The corn flour pre-treated with nano-enzymatic hydrolysis and the soybean meal pre-treated with ultrasound-assisted alkali treatment release nutrients more fully, providing a rich source of nutrients for the growth of black fungus. Furthermore, the novel bioactive synergist Y enhances the activity of key enzymes, promoting the metabolism and growth of black fungus. Experimental verification shows that this method significantly increases black fungus biomass and yield compared to traditional methods.

[0031] 2. The strain, screened through space-induced mutagenesis and domesticated in an oxygen-rich environment, boasts strong vitality and excellent genetic stability. Under the precise control of an intelligent feedback control system, the fermentation process is consistently optimized, ensuring consistent black fungus quality. Furthermore, advanced separation and extraction technologies significantly enhance product purity, boosting its market competitiveness.

[0032] 3. This method reduces pesticide use and environmental pollution. Furthermore, the efficient fermentation process and precise nutrient utilization shorten the fermentation cycle and lower production costs. For example, the fermentation cycle is shortened compared to traditional methods, saving energy and labor costs.

[0033] 4. The present invention demonstrates a high degree of innovation and intelligence in the production process. An intelligent feedback control system monitors and adjusts fermentation parameters in real time to ensure that fermentation is always in optimal condition. The application of advanced technologies such as efficient cross-flow membrane separation, freeze-drying combined with supercritical carbon dioxide extraction, and aqueous two-phase extraction combined with chromatographic separation not only improves the extraction rate and purity of the product, but also achieves the effective extraction of a variety of high-value-added ingredients in black fungus, laying the foundation for the diversified development of products and promoting the development of the black fungus industry towards high-end and intelligent directions. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is a flow chart of an industrial production method of black fungus liquid submerged fermentation;

[0035] Figure 2 It is a bar chart comparing the comprehensive performance of the embodiment and the comparative example;

[0036] Figure 31. It is a line graph comparing the production cost and product qualification rate of the embodiment and the comparative example;

[0037] Figure 4 It is a bar chart comparing the cost-effectiveness of the embodiment and the comparative example. DETAILED DESCRIPTION

[0038] according to Figures 1 to 4 , the specific implementation of the present invention is as follows:

[0039] Example 1

[0040] Raw material preparation and pretreatment: Take 25kg of corn flour, add nanocellulase and amylase (compound ratio 3.5:1), and enzymatically hydrolyze at 48°C for 2.5 hours. At the same time, weigh 20kg of soybean meal powder and place it in a solution with an ultrasonic power of 120W and an alkali concentration of 0.8% for 1.2 hours. After the treatment is completed, 25kg of enzymatically hydrolyzed corn flour and 20kg of treated soybean meal powder are mixed with 7kg of glucose, 5kg of yeast extract, 1.2kg of potassium dihydrogen phosphate, 0.6kg of magnesium sulfate, 0.12kg of zinc sulfate, and 0.03kg of vitamin B1. Then add 0.04kg of an organic high molecular weight polymer containing selenium and germanium (new bioactive synergist Y), add water and stir evenly, and adjust the pH to 6.0 with dilute hydrochloric acid and sodium hydroxide solution.

[0041] Sterilization and inoculation: put the prepared culture medium into the stainless steel fermentation tank (volume 10m 3 ), sterilized by autoclaving at 124°C for 28 minutes. After cooling to 27°C, inoculate the culture medium with a black fungus strain that has been screened by space mutagenesis and acclimated for 5 generations in an oxygen-rich environment (28%), at an inoculum size of 7% of the culture medium volume.

[0042] Fermentation process: Start the fermenter, control the temperature at 25°C, the stirring speed at 200 r / min, and the ventilation volume at 1.0 vvm. Dissolved oxygen, pH, biomass, and other data are collected every 12 minutes through an intelligent feedback control system. According to a preset algorithm, when the dissolved oxygen falls below 40%, the ventilation volume is increased; when the pH falls below 6.0, a carbon source solution containing xylose, glucose, and sucrose (mass ratio of 3:2:1) and a nitrogen source solution containing peptone, yeast extract powder, and ammonium sulfate (mass ratio of 2:1:1) are added to maintain the dissolved oxygen at around 42% and the pH at 6.05. Fermentation is continued for 6.5 days.

[0043] Separation and Extraction: After fermentation, the fermentation broth was introduced into a high-efficiency cross-flow membrane separation device (membrane pore size 0.15μm, operating pressure 0.2MPa, cross-flow velocity 1.5m / s) for solid-liquid separation. The obtained mycelium was first freeze-dried at -35°C for 9 hours, then transferred to a supercritical carbon dioxide extraction device and extracted for 1.5 hours at a pressure of 12MPa and a temperature of 38°C. The fermentation supernatant was extracted with a two-phase aqueous system consisting of polyethylene glycol (molecular weight 5000) and dextran (molecular weight 12000, mass ratio of 1.2:1), and then separated using a gel permeation chromatography column with a column length of 60cm and an inner diameter of 1.5cm. The eluent was 0.1mol / L phosphate buffer at a flow rate of 0.8mL / min, ultimately obtaining high-purity black fungus mycelium extract and polysaccharide extract.

[0044] Example 2

[0045] Raw material preparation and pretreatment: 23 kg of corn flour was enzymatically hydrolyzed with nanocellulase and amylase (compound ratio 3:1) at 46°C for 2 hours. 21 kg of soybean meal was ultrasonically treated at 100 W and 0.6% alkali for 1 hour. 23 kg of enzymatically hydrolyzed corn flour and 21 kg of treated soybean meal were mixed, and 6 kg of glucose, 4.5 kg of yeast extract, 1 kg of potassium dihydrogen phosphate, 0.5 kg of magnesium sulfate, 0.1 kg of zinc sulfate, 0.025 kg of vitamin B1, and 0.03 kg of a new bioactive synergist Y were added. Water was added to adjust the pH to 5.8.

[0046] Sterilization and inoculation: culture medium is filled into 5m 3 A stainless steel fermenter was sterilized by autoclaving at 123° C. for 25 minutes, cooled to 26° C., and inoculated with a strain that had been acclimated for 6 generations in an oxygen-rich environment (oxygen content 26%), with an inoculation amount of 6%.

[0047] Fermentation process: The fermenter was maintained at 24°C, agitation at 180 rpm, and ventilation at 0.8 vvm. The intelligent system collected data every 10 minutes and controlled the fermentation algorithm to maintain a dissolved oxygen content of 40% and a pH of 5.9 for six days.

[0048] Separation and Extraction: Cross-flow membrane separation was performed using a 0.1 μm pore size, an operating pressure of 0.15 MPa, and a cross-flow velocity of 1 m / s. Mycelia were freeze-dried at -40°C for 8 hours and subjected to supercritical carbon dioxide extraction at a pressure of 10 MPa, a temperature of 35°C, and a time of 1 hour. The supernatant was extracted with aqueous two-phase extraction using polyethylene glycol (molecular weight, 4000) and dextran (molecular weight, 10,000, mass ratio, 1:1). The extract was then separated by gel permeation chromatography on a 50 cm long, 1 cm internal diameter column at an eluent flow rate of 0.5 mL / min.

[0049] Example 3

[0050] Raw material preparation and pretreatment: 27 kg of corn flour was enzymatically hydrolyzed with nanocellulase and amylase (compound ratio 4:1) at 50°C for 3 hours. 19 kg of soybean meal was ultrasonically treated at 150 W and 1% alkali for 1.5 hours. After mixing, 8 kg of glucose, 5.5 kg of yeast extract, 1.5 kg of potassium dihydrogen phosphate, 0.8 kg of magnesium sulfate, 0.15 kg of zinc sulfate, and 0.04 kg of vitamin B1 were added. 0.05 kg of a new bioactive synergist Y was also added, and the pH was adjusted to 6.2.

[0051] Sterilization and inoculation: culture medium is filled into 20ml 3 A stainless steel fermenter was sterilized by autoclaving at 125° C. for 30 minutes, cooled to 28° C., and inoculated with a strain that had been acclimated for 7 generations in an oxygen-rich environment (oxygen content 30%), with an inoculation amount of 8%.

[0052] Fermentation process: Temperature controlled at 26°C, stirring at 220 rpm, and ventilation at 1.2 vvm. An intelligent system collected data every 15 minutes to maintain dissolved oxygen at 45% and a pH of 6.1 for 7 days.

[0053] Separation and extraction: Cross-flow membrane separation (pore size 0.2 μm, operating pressure 0.3 MPa, cross-flow velocity 2 m / s). Mycelia were freeze-dried at -30°C for 10 hours, and then subjected to supercritical carbon dioxide extraction at a pressure of 15 MPa, a temperature of 40°C, and a time of 2 hours. The supernatant was extracted with aqueous two-phase extraction using polyethylene glycol (molecular weight 6000) and dextran (molecular weight 15000, mass ratio 1.5:1). The product was then separated by gel permeation chromatography on an 80 cm long, 2 cm internal diameter column at an eluent flow rate of 1 mL / min.

[0054] Comparative Example

[0055] Raw material preparation and pretreatment: 25 kg of ordinary corn flour and 20 kg of ordinary soybean meal were taken without special pretreatment and directly mixed with 7 kg of glucose, 5 kg of yeast extract, 1.2 kg of potassium dihydrogen phosphate, 0.6 kg of magnesium sulfate, 0.12 kg of zinc sulfate, and 0.03 kg of vitamin B1. No new bioactive synergist Y was added, and the pH was adjusted to 6.0.

[0056] Sterilization and inoculation: culture medium is filled into 10m 3 A stainless steel fermentation tank was sterilized at 121°C for 20 minutes, cooled to 30°C, and inoculated with common black fungus at an inoculation rate of 5%.

[0057] Fermentation process: Fermentation tank temperature was 22°C, stirring speed was 150 r / min, and ventilation volume was 0.5 vvm. Fermentation was carried out according to fixed parameters without intelligent control for 5 days.

[0058] Separation and extraction: The product was obtained by separation using an ordinary plate and frame filter press, ordinary drying of the mycelium, and simple concentration of the fermentation supernatant without fine extraction.

[0059] Performance Testing

[0060] Examples 1, 2, and 3 are significantly superior to the control examples in terms of black fungus biomass, polysaccharide content, mycelium extract purity, and pesticide residues. Among them, the biomass of Example 1 reached 35 grams per liter, the polysaccharide content was 45%, the extract purity was 96%, the fermentation cycle was 6.5 days, and no pesticide residues were detected; the biomass of Example 2 was 32 grams per liter, the polysaccharide content was 42%, the extract purity was 95%, the fermentation cycle was 6 days, and no pesticide residues were detected; Example 3 performed best, with a biomass of up to 38 grams per liter, a polysaccharide content of 48%, an extract purity of 97%, a fermentation cycle of 7 days, and no pesticide residues. In contrast, although the fermentation cycle of the control example was shorter at only 5 days, the biomass was only 20 grams per liter, the polysaccharide content was 30%, the extract purity was 85%, and the pesticide residues due to pest control were as high as 0.5 mg per kilogram. It can be seen that the optimized culture medium technology of the present invention not only significantly improves the biomass, polysaccharide content and extract purity of black fungus, but also avoids the use of pesticides, ensuring the safety and high quality of the product. The comprehensive performance comparison of the embodiment and the comparative example is shown in the following table:

[0061] Table 1

[0062]

[0063]

[0064] Conclusion: This table comprehensively compares the performance of the Examples and Comparative Examples in various aspects of black fungus fermentation. The Examples significantly outperform the Comparative Examples in terms of biomass, nutrient content, fermentation cycle control, cost-effectiveness, and product quality, highlighting the superior advantages of the present invention.

[0065] The cost-effectiveness comparison of the embodiment and the comparative example is shown in the following table:

[0066] Table 2

[0067] Environmental indicators Example 1 Example 2 Example 3 Comparative Example Pesticide usage (kg / batch) 0 0 0 5 <![CDATA[Wastewater discharge volume (m 3 / batch)]]> 5 6 4 10 <![CDATA[Emission of waste gas (m 3 / batch, in terms of CO2 equivalent)]]> 20 22 18 30 Waste residue generation (kg / batch) 10 12 8 20 Resource utilization (%) 85 83 88 60

[0068] Conclusion: This table focuses on environmental protection, clearly demonstrating the advantages of the Examples in terms of pesticide use, waste gas emissions, and resource utilization. The Examples achieve green production with low pollution and high resource utilization, while the Comparative Examples exhibit significant environmental disadvantages, further demonstrating the sustainability of the present invention's technology.

[0069] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An innovative black fungus liquid submerged fermentation industrial production method, characterized in that: First, in the culture medium preparation step, 22-28 parts by weight of corn flour pretreated with nanoenzymatic hydrolysis, using a nanocellulase and amylase composite system during the pretreatment process; 18-22 parts of soybean meal powder treated with ultrasound-assisted alkali, the reaction formula during the treatment is: 6-8 parts of glucose, 4-6 parts of yeast extract, 0.8-1.5 parts of potassium dihydrogen phosphate, 0.4-0.8 parts of magnesium sulfate, 0.08-0.15 parts of zinc sulfate, and 0.02-0.04 parts of vitamin B1 are mixed; at the same time, a new bioactive synergist Y is added, which is an organic high molecular polymer containing selenium and germanium, and has the chemical formula: Wherein R and R' are organic groups, and the addition amount is 0.02-0.06 parts; add appropriate amount of water and adjust the pH to 5.7-6.3; The subsequent sterilization is carried out by high-pressure sterilization at 123-125°C for 25-30 minutes; after cooling to 26-28°C, black fungus strains screened by space mutagenesis and domesticated in an oxygen-rich environment are inoculated at a volume ratio of 6%-8%; fermentation is carried out in a fermenter with an intelligent feedback control system at a temperature of 24-26°C, a stirring speed of 180-220 r / min, a ventilation volume of 0.8-1.2 vvm, online monitoring of dissolved oxygen, pH, and biomass, and intelligent control of the feed containing a specially formulated carbon and nitrogen source. The carbon source is a mixture of xylose, glucose, and sucrose in a mass ratio of 3:2:1, and the nitrogen source is a mixture of peptone, yeast extract powder, and ammonium sulfate in a mass ratio of 2:1:

1. The dissolved oxygen is maintained at 40%-45%, the pH is 6.0-6.1, and the fermentation is carried out for 6-7 days; After fermentation, the product is separated by a high-efficiency cross-flow membrane separation device, and the mycelium is treated by freeze-drying combined with supercritical carbon dioxide extraction technology, first freeze-dried at -40-30℃, and then extracted under supercritical carbon dioxide pressure of 10-15MPa and temperature of 35-40℃; the fermentation supernatant is extracted by two-phase aqueous extraction combined with chromatographic separation technology to extract the target components, and subsequent chromatographic separation uses gel permeation chromatography column.

2. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein When pretreating corn flour with nanoenzymatic hydrolysis, the composite ratio of nanocellulase and amylase is 3:2-4:1, the enzymatic hydrolysis temperature is 45-50°C, and the time is 2-3 hours.

3. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein When ultrasonic-assisted alkali treatment of soybean meal powder is performed, the ultrasonic power is 100-150W, the alkali concentration is 0.5%-1%, and the treatment time is 1-1.5 hours.

4. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein The black fungus strains screened by space mutagenesis and domesticated in an oxygen-rich environment have their activity increased by 30%-40% after 5-7 generations of domestication in an oxygen-rich environment.

5. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein The intelligent feedback control system monitors fermentation parameters in real time through sensors, collects data every 10-15 minutes, and adjusts the feed rate and ventilation volume according to the preset algorithm.

6. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein The membrane pore size of the high-efficiency cross-flow membrane separation device is 0.1-0.2μm, the operating pressure is 0.1-0.3MPa, and the cross-flow velocity is 1-2m / s.

7. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein When freeze drying is combined with supercritical carbon dioxide extraction technology to treat mycelium, the freeze drying time is 8-10 hours and the supercritical carbon dioxide extraction time is 1-2 hours.

8. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein When the fermentation supernatant is extracted by aqueous two-phase extraction, the molecular weight of polyethylene glycol is 4000-6000, the molecular weight of dextran is 10000-15000, and the mass ratio of the two is 1:1-1.5:

1.

9. The black fungus liquid submerged fermentation industrial production method according to claim 1, wherein The gel permeation chromatography column has a length of 50-80 cm, an inner diameter of 1-2 cm, an eluent of 0.1 mol / L phosphate buffer, and a flow rate of 0.5-1 mL / min.

10. The black fungus liquid submerged fermentation industrial production method according to claim 1, characterized in that: The purity of the final products, black fungus mycelium extract and polysaccharide extract, is not less than 95%.

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