Alcohol effect dispelling and liver protecting preparation containing bifidobacterium longum subsp.infantis and preparation method thereof

Abstract

The invention belongs to the technical field of healthy food, and particularly provides a hangover-alleviating and liver-protecting preparation containing bifidobacterium longum subsp.infantis and a preparation method of the hangover-alleviating and liver-protecting preparation, and the hangover-alleviating and liver-protecting preparation is prepared from the following raw materials in parts by weight: 20-40 parts of plant extract, 8-18 parts of compound probiotics, 3-7 parts of prebiotics and 10-20 parts of antioxidant. According to the preparation for dispelling the effects of alcohol and protecting the liver, the compound probiotics and the plant extract are scientifically and reasonably matched for use, the components are natural and high in safety, all the components are in synergistic interaction, and the preparation has the effects of dispelling the effects of alcohol, protecting the liver and improving the body health condition.

Description

Technical Field

[0001] The invention belongs to the technical field of health foods, and particularly relates to a hangover-relief and liver-protecting preparation containing Bifidobacterium longum subspecies infantis and a preparation method thereof. Background Art

[0002] With the development of society and the improvement of people's living standards, drinking has become a common social activity. However, excessive drinking can cause many adverse effects on human health, especially serious damage to the liver. The liver is an important metabolic and detoxification organ in the human body. After alcohol enters the body, it is mainly metabolized and broken down in the liver. Alcohol and its metabolite acetaldehyde can have toxic effects on liver cells, causing liver cell damage and inflammatory reactions. Long-term and heavy drinking may also induce alcoholic liver disease, such as fatty liver, alcoholic hepatitis, liver fibrosis, and even cirrhosis.

[0003] China has a deep drinking culture, so people hope to improve the hangover feeling after drinking through functional preparations for alcohol detoxification, while alleviating the damage to the liver caused by alcohol or improving the individual's ability to metabolize alcohol. Currently, there are some alcohol detoxification and liver protection products on the market, each with its own limitations and limited effectiveness, and they are unable to fundamentally reduce the damage to the liver caused by alcohol and the adverse effects on other systems of the body. In recent years, more and more probiotics have been found to have alcohol detoxification and liver protection effects. Among them, Bifidobacterium longum subsp. infantis can not only help metabolize alcohol and protect intestinal health, but also reduce liver inflammation and protect liver cells. In view of the shortcomings of existing alcohol detoxification and liver protection methods and the many benefits of Bifidobacterium longum subsp. infantis, the development of an alcohol detoxification and liver protection preparation containing Bifidobacterium longum subsp. infantis has important practical significance.

[0004] Chinese patent CN 115044518 B discloses a liver-protecting probiotic and its application. Bifidobacterium longum W68, deposited with CGMCC No. 15028, has a significant effect on repairing liver damage. When used as a sustained-release liver-protecting probiotic tablet, its ingredients are as follows: a traditional Chinese medicine fermentation product (fermented by Bifidobacterium longum W68 on a traditional Chinese medicine extract), konjac glucomannan, sodium alginate, lactose, microcrystalline cellulose, magnesium stearate, sodium lauryl sulfate, cross-linked polyvinylpyrrolidone, cross-linked sodium carboxymethyl cellulose, and sodium bicarbonate. However, the ingredients of these liver-protecting probiotic sustained-release tablets are complex, increasing the cost of alcohol-detoxifying products and uncontrollable ingredients, leading to uncertainty in their effectiveness in detoxifying and protecting the liver.

[0005] Therefore, there is an urgent need for a hangover-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis, which is expected to exert the effects of hangover-detoxification and liver protection through the multiple biological functions of Bifidobacterium longum subsp. infantis combined with plant ingredients. Summary of the Invention

[0006] In response to existing technical problems, the present invention aims to provide a hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a method for preparing the preparation. This hangover-relief and liver-protecting preparation scientifically and rationally combines a composite probiotic with a plant extract. The ingredients are natural, highly safe, and synergistic, resulting in hangover-relief, liver-protection, and improved health.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] On the one hand, the present invention provides a hangover-relief and liver-protecting preparation containing Bifidobacterium longum subspecies infantis, which comprises the following raw materials in parts by weight: 20-40 parts of plant extract, 8-18 parts of compound probiotics, 3-7 parts of prebiotics, and 10-20 parts of antioxidants.

[0009] In some embodiments, the plant extract is a combination of Pueraria root extract, Hovenia dulcis fruit extract, Camellia sinensis extract, and Curcuma longa extract.

[0010] The present invention synergistically combines Pueraria root extract, Hovenia dulcis fruit extract, Camellia sinensis extract, and Curcuma longa extract to relieve alcohol and protect the liver, thereby producing a synergistic effect. It also reduces the adverse reactions that may occur when a single ingredient is used, thereby improving the safety and tolerability of the preparation. The reasons for this may be:

[0011] First, Hovenia dulcis extract, like Pueraria root extract, can increase the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) in the liver, thereby accelerating alcohol metabolism. The combined effect is significant. Flavonoids in Tengcha extract also have a certain promoting effect on alcohol metabolism.

[0012] Secondly, the flavonoids in Pueraria root extract possess strong antioxidant properties, scavenging free radicals in the body, alleviating alcohol-induced oxidative stress, and protecting liver cells. Hovenia dulcis fruit extract can also increase superoxide dismutase (SOD) activity in the liver, reduce malondialdehyde (MDA) levels, and enhance the liver's antioxidant capacity. The active ingredients in vine tea extract and turmeric extract also possess significant antioxidant effects, further mitigating alcohol-induced oxidative damage to the liver.

[0013] In some embodiments, the mass ratio of the Pueraria root extract, the Hovenia dulcis fruit extract, the Ampelopsis pilosula extract and the Curcuma longa extract is (5-10):(8-16):(2-4):1.

[0014] In some embodiments, the method for preparing the Pueraria root extract comprises the following steps:

[0015] R1. Cut the kudzu root into pieces, immerse in distilled water, heat to 50-60°C, filter, concentrate, dry, and grind to obtain kudzu root powder;

[0016] R2. Mix the oligopeptide and distilled water, adjust the pH value to 10-12, add the Pueraria powder obtained in step R1 while stirring, continue stirring for 2-4 hours, adjust the pH value to 6-7, centrifuge, collect the supernatant, and dry to obtain the Pueraria extract.

[0017] Ingredients such as daidzein in Pueraria extract can slow down the absorption of alcohol by the gastrointestinal tract, thereby reducing the ethanol concentration in the blood, improving the body's tolerance to alcohol, reducing the amount of alcohol entering the blood circulation, and thus reducing the stimulation and damage of alcohol to organs throughout the body; at the same time, flavonoids in Pueraria extract can significantly increase the activity of liver alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). ADH can convert ethanol into acetaldehyde, and ALDH can convert acetaldehyde into acetic acid. Acetic acid is eventually decomposed into carbon dioxide and water and excreted from the body, thereby accelerating the metabolism of alcohol, allowing the accumulated alcohol in the body to be cleared more quickly, shortening the time of drunkenness, and alleviating the symptoms of discomfort after drinking.

[0018] Furthermore, ingredients like daidzein and daidzin in Pueraria root extract have a protective effect on liver cells, enhancing their regenerative capacity, helping to repair damaged liver cells, restoring normal liver function, and maintaining the liver's normal metabolic and detoxification functions, thereby better addressing alcohol-induced liver damage. Pueraria root extract can also reduce liver fat content, improve the liver's lipid metabolism, and prevent and alleviate alcohol-induced fatty liver disease. Furthermore, Pueraria root extract can regulate abnormalities in liver enzymes, promoting glucose, fat, and protein metabolism, and enhancing the liver's detoxification and metabolic functions, allowing the liver to better perform its physiological functions and reducing the burden of alcohol on the liver.

[0019] However, the oral bioavailability of conventional kudzu root extracts is low. Based on this, the present invention first extracts kudzu root powder with distilled water, and then uses an alkaline environment to improve the solubility of kudzu root powder, and successfully loads it on oligopeptides to prepare kudzu root extracts. Oligopeptides themselves have certain biological activities, can regulate intestinal flora, enhance immunity, and after being mixed and modified with kudzu root powder, they can produce a synergistic effect with the effective ingredients in kudzu root, further enhancing the medicinal properties of kudzu root extracts. Compared with directly adding commercially available kudzu root extracts and oligopeptides, the kudzu root extract prepared by the present application improves its oral bioavailability, thereby making the hangover and liver protection preparations more effective.

[0020] In some embodiments, the oligopeptide in step R2 is soybean oligopeptide and / or corn oligopeptide.

[0021] In some embodiments, the mass ratio of the kudzu root powder to the oligopeptide in step R2 is 1:(1-2).

[0022] In some embodiments, the composite probiotic is a combination of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus, and Bifidobacterium adolescentis.

[0023] Preferably, the mass ratio of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis is (4-8):(3-5):1.

[0024] When Bifidobacterium longum infantis subspecies, Lactobacillus rhamnosus and Bifidobacterium adolescentis act together, a synergistic effect can be produced by regulating and controlling its specific ratio. Lactobacillus rhamnosus can reduce the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in serum, reduce triglycerides and reduced glutathione in the liver, thereby alleviating the oxidative damage of alcohol to the liver, delaying the formation of alcoholic fatty liver, and it can also inhibit the expression of ethanol-induced P2X4 and P2X7 genes, thereby alleviating symptoms of drunkenness. Bifidobacterium longum infantis subspecies and Bifidobacterium adolescentis can regulate intestinal flora, inhibit the generation of inflammatory factors, and alleviate the inflammatory response of the liver. Simultaneously, these composite probiotics can increase the short-chain fatty acids (SCFA) content in the intestinal tract by regulating intestinal flora, thereby improving the antioxidant capacity of the liver.

[0025] In some embodiments, the prebiotics include any one or more of fructooligosaccharides, xylan, inulin, mannose oligosaccharides, xylo-oligosaccharides, and isomaltooligosaccharides.

[0026] In some embodiments, the antioxidant is vitamin C and / or a vitamin C derivative.

[0027] In some embodiments, the preparation for detoxifying and protecting the liver is in the form of granules, powders, tablets or capsules.

[0028] Another aspect of the present invention provides a method for preparing a hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis, comprising the following steps:

[0029] The plant extract, compound probiotics, prebiotics and antioxidants are mixed, stirred, crushed and formed to obtain a hangover-relieving and liver-protecting preparation.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The alcohol-relieving and liver-protecting preparation of the present invention scientifically and rationally combines compound probiotics and plant extracts. The ingredients are natural and highly safe. The ingredients synergize with each other, have the effects of relieving alcohol, protecting the liver, and improving physical health, and have good market value.

[0032] 2. Ordinary kudzu root extract has low oral bioavailability. The kudzu root extract prepared by modifying with oligopeptides in the present invention not only improves oral bioavailability, but also enhances the medicinal properties of the kudzu root extract due to the synergistic effect of the biological activity of oligopeptides and the effective ingredients of kudzu root, making the alcohol-detoxifying and liver-protecting preparation more effective.

[0033] 3. The Pueraria root extract, Hovenia dulcis fruit extract, Camellia sinensis extract and Curcuma longa extract of the present invention are used together in combination to detoxify and protect the liver, which can synergistically enhance the effect, reduce adverse reactions, and improve the safety and tolerability of the detoxification and liver protection preparation.

[0034] 4. The Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis of the present invention can synergistically enhance efficacy when acting together, relieve symptoms of drunkenness, reduce oxidative damage of alcohol to the liver, regulate intestinal flora, and inhibit the production of inflammatory factors. DETAILED DESCRIPTION

[0035] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following examples are illustrative of the present invention and are intended only to illustrate the present invention, not to limit the present invention. Other combinations and various modifications within the scope of the present invention may be made without departing from the spirit or scope of the present invention.

[0036] According to the ratios of the raw materials and the preparation methods specified in the following examples and comparative examples, a hangover-relieving and liver-protecting preparation was prepared.

[0037] In order to facilitate those skilled in the art to implement the present invention, the sources of some raw materials in the embodiments and comparative examples are described as follows:

[0038] Bifidobacterium longum subsp. infantis deposit information:

[0039] Deposit institution: Guangdong Provincial Microbiological Culture Collection, deposit date: September 26, 2021, deposit number: GDMCC No.: 1.2817;

[0040] Bifidobacterium adolescentis collection information:

[0041] The deposit institution is Guangdong Provincial Microbiological Culture Collection Center, the deposit date is November 25, 2017, and the deposit number is GDMCC No.: 1.1262.

[0042] Lactobacillus rhamnosus preservation information:

[0043] The deposit institution is Guangdong Provincial Microbiological Culture Collection, the deposit date is September 3, 2019, and the deposit number is GDMCC No.: 1.1732.

[0044] Soybean oligopeptides: purchased from Jiangsu Jiujia Biotechnology Co., Ltd.

[0045] Fructooligosaccharide: purchased from Guangdong Mingtong Biotechnology Co., Ltd.

[0046] Hovenia dulcis fruit extract, Camellia sinensis extract, Curcuma longa extract, and Pueraria root extract were purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0047] Unless otherwise specified, other raw materials can be purchased from the market.

[0048] Preparation Example 1

[0049] The preparation method of vitamin C derivatives comprises the following steps:

[0050] S1. Add 50 mL of tert-amyl alcohol to a reactor, then add 4.1 g of vitamin C and 4 g of azelaic acid. After stirring and dissolving, heat to 45°C, then add 0.3 g of lipase. Incubate at 45°C with shaking for 24 h. Evaporate the solvent by vacuum distillation to obtain the reactant.

[0051] S2. The reactant obtained in step S1 was poured into a mixture of ice water and ethyl acetate (the volume ratio of ice water to ethyl acetate was 1:0.3), stirred evenly, filtered, washed three times with 100 mL of water each time, and vacuum dried at 50°C to constant weight to obtain a vitamin C derivative.

[0052] Preparation Example 2

[0053] The preparation method of Pueraria root extract A comprises the following steps:

[0054] R1. Cut 250g of Pueraria root into pieces, immerse in 2500mL of distilled water, heat to 60°C, filter, concentrate, dry at 85°C to constant weight, crush and sieve to 30 mesh to obtain Pueraria root powder;

[0055] R2. Mix 150 g of soybean oligopeptides and 1500 mL of distilled water, adjust the pH to 11, add 100 g of Pueraria powder obtained in step R1 while stirring, continue stirring for 3 h, adjust the pH to 6.5, centrifuge for 10 min, take the supernatant, and dry at 85°C to constant weight to obtain Pueraria extract A.

[0056] Preparation Example 3

[0057] The preparation method of Pueraria lobata extract B is the same as that of Preparation Example 2, except that the amount of soybean oligopeptide added in step R2 is 90 g.

[0058] Example 1

[0059] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subspecies infantis, comprising the following raw materials in parts by weight:

[0060] 30 parts of plant extract, 13 parts of compound probiotics, 5 parts of oligofructose, 15 parts of vitamin C derivatives, including:

[0061] The plant extract consists of Pueraria root extract A, Hovenia dulcis fruit extract, Camellia sinensis extract and Curcuma longa extract in a mass ratio of 7.5:12:3:1;

[0062] The compound probiotics consist of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis in a mass ratio of 6:4:1.

[0063] The preparation method of the alcohol-relieving and liver-protecting preparation of this embodiment comprises the following steps:

[0064] The plant extract, compound probiotics, oligofructose and vitamin C derivative are mixed, stirred evenly, crushed and formed to obtain a hangover-relieving and liver-protecting preparation.

[0065] Example 2

[0066] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subspecies infantis, comprising the following raw materials in parts by weight:

[0067] 20 parts of plant extract, 8 parts of compound probiotics, 3 parts of oligofructose, 10 parts of vitamin C derivatives, including:

[0068] The plant extract consists of kudzu root extract A, Hovenia dulcis fruit extract, Camellia sinensis extract and Curcuma longa extract in a mass ratio of 5:8:2:1;

[0069] The compound probiotics consist of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis in a mass ratio of 4:3:1.

[0070] The preparation method of the alcohol-relieving and liver-protecting preparation of this embodiment is the same as that of Example 1.

[0071] Example 3

[0072] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subspecies infantis, comprising the following raw materials in parts by weight:

[0073] 40 parts of plant extract, 18 parts of compound probiotics, 7 parts of oligofructose, 20 parts of vitamin C derivatives, including:

[0074] The plant extract consists of kudzu root extract A, Hovenia dulcis fruit extract, Camellia sinensis extract and Curcuma longa extract in a mass ratio of 10:16:4:1;

[0075] The compound probiotics consist of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis in a mass ratio of 8:5:1.

[0076] The preparation method of the alcohol-relieving and liver-protecting preparation of this embodiment is the same as that of Example 1.

[0077] Example 4

[0078] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of Pueraria root extract B is used instead of Pueraria root extract A.

[0079] Example 5

[0080] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of Pueraria root extract A is used instead of the plant extract.

[0081] Example 6

[0082] A hangover-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that the plant extract comprises Pueraria root extract A, Hovenia dulcis fruit extract, Camellia sinensis extract, and Curcuma longa extract in a mass ratio of 4:12:3:1.

[0083] Example 7

[0084] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that the composite probiotic comprises Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis in a mass ratio of 3:4:1.

[0085] Example 8

[0086] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of commercially available Pueraria lobata extract is used instead of Pueraria lobata extract A.

[0087] Comparative Example 1

[0088] A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of Bifidobacterium longum subsp. infantis is used instead of the composite probiotics.

[0089] Effect evaluation:

[0090] The alcohol-relieving and liver-protecting preparations prepared in Examples 1-8 and Comparative Example 1 were tested, and the specific results are shown in Table 1.

[0091] Performance testing:

[0092] A number of ICR mice weighing 18 to 22 g were randomly divided into multiple groups, with 8 mice in each group and the average weight difference between the mice in each group was no more than 0.5 g. The mice were administered once a day at a concentration of 0.50 g / kg. 30 minutes after each administration, except for the blank control group which was gavaged with an equal volume of distilled water, the mice in each embodiment and comparative example group were gavaged with the optimal alcohol dose for 7 consecutive days. At the end of the experiment, the mice in each group were fasted for 4 hours and weighed. The mice were sacrificed on the 8th day, and the livers were quickly removed, immediately rinsed with pre-cooled physiological saline, blotted dry with filter paper, and weighed. The liver index was calculated; liver index / % = mouse liver weight / mouse body weight × 100%;

[0093] Finally, a small amount of liver tissue was cut, added with 9 times the volume of normal saline, homogenized on ice, and centrifuged at 120,000×g, 4°C for 5 min to obtain the liver tissue supernatant, and the ADH and ALDH levels in the mouse liver tissue were measured.

[0094] Table 1

[0095]

[0096]

[0097] The results in Table 1 show that the alcohol-detoxifying and liver-protecting preparations prepared in Examples 1-3 can effectively reduce the liver index, significantly increase the levels of ADH and ALDH, and can increase the activity of the body's catalase, thereby accelerating alcohol metabolism and reducing the ethanol content in the blood, achieving a good alcohol-detoxifying and liver-protecting effect.

[0098] Compared with Example 1, in Example 4, the mass ratio of Pueraria powder and oligopeptides was changed when preparing the Pueraria extract, resulting in poor loading effect; at the same time, compared with Example 1, in Example 8, an equal amount of commercially available Pueraria extract was used to replace Pueraria extract A. All of the above will further reduce the bioavailability of the Pueraria extract, affecting the alcohol-relieving and liver-protecting effects.

[0099] Compared with Example 1, when preparing the hangover-relieving and liver-protecting preparation, Example 5 changed the type of plant extract and only used Pueraria extract A; Example 6 changed the mass ratio of Pueraria extract, Hovenia dulcis extract A, Camellia sinensis extract and Curcuma longa extract in the plant extracts. All of the above will further weaken the synergistic effect of hangover-relieving and liver-protecting, affecting the hangover-relieving and liver-protecting effect.

[0100] Compared with Example 1, when preparing the hangover-relieving and liver-protecting preparation, Example 7 changed the mass ratio of Bifidobacterium longum subsp. infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis in the composite probiotics; at the same time, compared with Example 1, Comparative Example 1 changed the type of composite probiotics and only used Bifidobacterium longum subsp. infantis. All of the above will further weaken the synergistic effect of hangover-relieving and liver-protecting, affecting the hangover-relieving and liver-protecting effect.

[0101] The above is only a preferred embodiment of the present invention and does not limit the present application in any form. Although the present application is disclosed as above with a preferred embodiment, it is not intended to limit the present application. Any technician familiar with this profession, without departing from the scope of the technical solution of the present application, makes some changes or modifications using the technical content disclosed above, which are equivalent to equivalent implementation cases. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution.

Claims

1. A hangover-relief and liver-protecting preparation containing Bifidobacterium longum subsp. infantis, characterized in that: The invention comprises the following raw materials in parts by weight: 20-40 parts of plant extract, 8-18 parts of compound probiotics, 3-7 parts of prebiotics, and 10-20 parts of antioxidants.

2. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 1, characterized in that: The plant extract is a composition of kudzu root extract, hovenia dulcis fruit extract, truncatum ulmoides extract and turmeric extract.

3. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 2, characterized in that: The mass ratio of the kudzu root extract, the hovenia dulcis fruit extract, the truncatum ulmoides extract and the turmeric extract is (5-10): (8-16): (2-4):

1.

4. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 2, characterized in that: The preparation method of the Pueraria root extract comprises the following steps: R1. Cut the kudzu root into pieces, immerse in distilled water, heat to 50-60°C, filter, concentrate, dry, and grind to obtain kudzu root powder; R2. Mix the oligopeptide and distilled water, adjust the pH value to 10-12, add the Pueraria powder obtained in step R1 while stirring, continue stirring for 2-4 hours, adjust the pH value to 6-7, centrifuge, collect the supernatant, and dry to obtain the Pueraria extract.

5. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 4, characterized in that: The oligopeptide in step R2 is soybean oligopeptide and / or corn oligopeptide.

6. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 4, characterized in that: The mass ratio of the kudzu root powder to the oligopeptide in step R2 is 1:(1-2).

7. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 1, characterized in that: The compound probiotics are a composition of Bifidobacterium longum subspecies infantis, Lactobacillus rhamnosus and Bifidobacterium adolescentis.

8. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 1, characterized in that: The antioxidant is vitamin C or a vitamin C derivative.

9. The alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to claim 1, characterized in that: The preparation for alcohol detoxification and liver protection is in the form of granules, powders, tablets or capsules.

10. A method for preparing the alcohol-detoxifying and liver-protecting preparation containing Bifidobacterium longum subsp. infantis according to any one of claims 1 to 9, characterized in that: The following steps are included: The plant extract, compound probiotics, prebiotics and antioxidants are mixed, stirred, crushed and formed to obtain a hangover-relieving and liver-protecting preparation.

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