Whey protein probiotic freeze-dried powder capable of reducing cholesterol as well as preparation method and application of whey protein probiotic freeze-dried powder

Abstract

The invention relates to the field of probiotic freeze-dried powder, and provides whey protein probiotic freeze-dried powder capable of reducing cholesterol as well as a preparation method and application thereof.Probiotics are lactobacillus paracasei, the lactobacillus paracasei is added into a whey fermentation culture medium to be fermented and centrifugally separated to remove supernate to obtain protein bacterial sludge, xylitol, VC, trehalose and water are added, and the whey protein probiotic freeze-dried powder capable of reducing cholesterol is obtained. And uniformly shearing the mixture, pre-freezing, and carrying out vacuum freeze-drying to obtain the whey protein probiotic freeze-dried powder. The method greatly reduces the production cost, is suitable for large-scale industrial production, and realizes the resource reutilization of whey. The final product is rich in protein nutrition, and can provide abundant nutritional ingredients for the growth of the probiotics, reduce the activity loss of the probiotics and ensure the activity and stability of the probiotics, so that the effects of the probiotics can be kept for a longer time, and a relatively high survival rate can be kept in the transportation and storage processes. The obtained freeze-dried powder can reduce cholesterol and can be used for preparing products for reducing cholesterol.

Description

Technical Field

[0001] The present invention relates to the field of probiotic freeze-dried powder, and in particular to a whey protein probiotic freeze-dried powder for reducing cholesterol, and a preparation method and application thereof. Background Art

[0002] Whey is a byproduct of the industrial production of cheese and casein. During cheese production, only about 10% of raw milk is made into cheese, while the remaining 90% is discarded as whey. Whey is divided into two types: sweet whey (SW) and acid whey (AW). Whey contains a large amount of amino acids, protein, vitamins, and carbohydrates, making it a nutritionally valuable food ingredient. However, whey is underutilized in the food industry. Of all whey discharged worldwide, only 59% is used for industrial purposes, while the remaining 41% is either used as livestock feed or agricultural fertilizer or discarded as industrial waste. Therefore, effectively utilizing whey and expanding its applications are urgent issues for dairy manufacturers.

[0003] Cholesterol is an essential component of human tissues and cells, primarily synthesized by the body itself, with a small portion derived from dietary intake. A moderate amount of cholesterol is crucial for maintaining normal physiological functions. Excessive levels of total cholesterol, triglycerides, and low-density lipoprotein (LDL) in serum can lead to hyperlipidemia, which can cause coronary heart disease, myocardial infarction, cerebral infarction, hypertension, and other conditions, ultimately impacting health.

[0004] Probiotics are a class of living microorganisms that benefit the host, helping to maintain a balanced intestinal flora, promote digestion and absorption, enhance immunity, and even potentially improve certain diseases. Some probiotics, such as Lactobacillus plantarum, Lactobacillus paracasei, Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus rhamnosus, as well as fermented products containing probiotics, have cholesterol-lowering and lipid-regulating effects. The development of cholesterol-lowering probiotic powders is of great significance. However, the activity of probiotics is easily affected by the external environment, making it a significant challenge to maintain their activity during storage and transportation. With increasing health awareness, freeze-dried probiotic powders have garnered widespread attention for their unique health benefits. Freeze-dried probiotic powders are powdered probiotics produced through freeze-drying. This process effectively maintains the activity and stability of the probiotics, facilitating storage and transportation. This method maximizes the activity of the probiotics, as the low temperature and lack of oxygen minimize cellular damage. With the advancement of scientific research and the growing consumer demand for health products, freeze-dried probiotic powders are becoming increasingly important in the market. It can not only be eaten directly as a health product, but also can be used as an additive in the production of food, beverages and health products.

[0005] Currently, whey wastewater treatment is associated with issues such as residual chemical reagents, the discharge of highly polluted wastewater, waste of resources such as lactose, and process safety. Sweet whey processing requires supporting membrane separation, fermentation tanks, and drying equipment, resulting in high investment costs. Existing technologies also suffer from low viable counts of probiotics and low survival rates during freezing and shelf life. Currently, there is a lack of a method for culturing and fermenting probiotics using whey that retains the nutritional value of whey protein while delivering the benefits of probiotics and ensuring microbial activity. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a whey protein probiotic freeze-dried powder for lowering cholesterol, and a preparation method and application thereof. The probiotic freeze-dried powder can improve intestinal flora, lower cholesterol, and overcome the problems of low viable bacteria count and low survival rate during freezing and shelf life in probiotics in the prior art.

[0007] In order to achieve the above object, the present invention provides a method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder, comprising the following steps:

[0008] (1) pasteurizing the whey culture medium to obtain a whey fermentation medium;

[0009] (2) adding the activated Lactobacillus paracasei strain into a whey fermentation medium for fermentation;

[0010] (3) centrifuging the fermented whey and removing the supernatant to obtain protein sludge;

[0011] (4) Add 0.5-2 parts of xylitol, 0.5-2 parts of VC, 1-2 parts of trehalose, and 50-100 parts of water to 10-20 parts of protein mud. Shear the mixture evenly and pre-freeze it. After pre-freezing, vacuum freeze-dry it to obtain whey protein probiotic freeze-dried powder.

[0012] Preferably, in step (1), the pasteurization conditions of the whey culture medium are 90-95° C. for 10-15 min.

[0013] Preferably, in step (2), the volume ratio of the inoculation amount of Lactobacillus paracasei is 1-3%, the fermentation time is 10-18 h, and the fermentation temperature is 37-42°C.

[0014] Preferably, in step (3), the centrifugation parameters are 8000-12000 r / min, 10-30 min.

[0015] Preferably, in step (4), the shear parameter is 10000~12000 rpm, 3 min; the pre-freezing temperature is -20~-80℃, and the pre-freezing time is 8~24 h; the vacuum freeze-drying temperature is -50~-80℃, the vacuum freeze-drying pressure is 0.5~1kPa, and the vacuum freeze-drying time is 48~72 h.

[0016] In some embodiments, the pre-freezing temperature is -40~-80°C or -60~-80°C, and the pre-freezing time is 8~12 h or 12~24 h; the vacuum freeze-drying temperature is -50~-60°C or -50~-70°C, the vacuum freeze-drying pressure is 0.5~1 kPa, and the vacuum freeze-drying time is 60~72 h.

[0017] Another aspect of the present invention provides a whey protein probiotic freeze-dried powder for lowering cholesterol, which is prepared by the above-mentioned preparation method. In the present invention, Lactobacillus paracasei is a Gram-positive bacterium, a type of probiotic, and has multiple benefits for human health. Lactobacillus paracasei can colonize in the intestines, help maintain the balance of intestinal microflora, inhibit the growth of harmful bacteria, and promote intestinal health. Lactobacillus paracasei can also stimulate the activity of macrophages and NK cells, can enhance the body's immunity, and help resist the invasion of pathogens. In addition, Lactobacillus paracasei can also assist in lowering cholesterol and improving blood lipid metabolism; and can produce a variety of beneficial substances, which have a positive promoting effect on the digestion, absorption and nutritional status of the human body.

[0018] In another aspect, the present invention provides an application of a cholesterol-lowering whey protein probiotic freeze-dried powder for preparing a cholesterol-lowering product; the product comprises one or more of a medicine, a nutrient or a health product.

[0019] The product also includes acceptable excipients.

[0020] Another aspect of the present invention provides a product; the product contains the cholesterol-lowering whey protein probiotic freeze-dried powder; the product includes one or more of medicines, nutritional products or health products.

[0021] Beneficial effects of the present invention:

[0022] Whey is a by-product of the cheese and other dairy industries. The present invention uses whey as a culture medium to ferment and culture probiotics to prepare probiotic freeze-dried powder. Whey has the advantages of low raw material cost and a wide source, which can significantly reduce production costs and is suitable for large-scale industrial production; it also achieves the resource recycling of whey. The final product is rich in protein nutrition. Xylitol, VC, and trehalose can provide rich nutrients for the growth of probiotics, reduce the loss of probiotic activity, ensure the activity and stability of probiotics, and enable probiotics to maintain their efficacy for a longer period of time, and maintain a high survival rate during transportation and storage. The Lactobacillus paracasei of the present invention is a lactic acid bacterium that can lower cholesterol. The probiotic powder has the function of improving intestinal flora and lowering cholesterol, and can be used to prepare products that improve intestinal flora and / or lower cholesterol. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a scanning electron microscope image of the whey protein probiotic freeze-dried powder of the present invention. DETAILED DESCRIPTION

[0024] The present invention is described in detail below with reference to the following examples. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used below were all obtained from commercial sources unless otherwise specified.

[0025] Example 1

[0026] The present embodiment provides a cholesterol-lowering whey protein probiotic freeze-dried powder, which is prepared by the following preparation method, comprising the steps of:

[0027] (1) pasteurizing the whey culture medium at 95°C for 15 min to obtain a whey fermentation medium;

[0028] (2) The activated Lactobacillus paracasei strain was inoculated into whey fermentation medium at a 1% inoculum rate and fermented at 37°C for 14 h;

[0029] (3) The fermented whey was centrifuged at 10,000 r / min for 15 min, and the supernatant was removed to obtain protein sludge.

[0030] (4) Add 1 part of xylitol, 1 part of VC, 1 part of trehalose and 50 parts of water to 10 parts of protein slurry. The mixture is sheared at 10000-12000 rpm for 3 min. After uniform shearing, it is quickly pre-frozen at a temperature of -80℃ for 12 h. After pre-freezing, it is vacuum freeze-dried at a temperature of -50℃, a pressure of 0.5-1 kPa and a time of 72 h to obtain whey protein probiotic freeze-dried powder.

[0031] Example 2

[0032] The present embodiment provides a cholesterol-lowering whey protein probiotic freeze-dried powder, which is prepared by the following preparation method, comprising the steps of:

[0033] (1) pasteurizing the whey culture medium at 95°C for 15 min to obtain a whey fermentation medium;

[0034] (2) The activated Lactobacillus paracasei strain was inoculated into whey fermentation medium at a 2% inoculum rate and fermented at 37°C for 10 h;

[0035] (3) The fermented whey was centrifuged at 10,000 r / min for 15 min, and the supernatant was removed to obtain protein sludge.

[0036] (4) Add 1 part of xylitol, 1 part of VC, 1 part of trehalose and 50 parts of water to 10 parts of protein slurry. The mixture is sheared at 10000-12000 rpm for 3 min. After uniform shearing, it is quickly pre-frozen at a temperature of -80℃ for 12 h. After pre-freezing, it is vacuum freeze-dried at a temperature of -50℃, a pressure of 0.5-1 kPa and a time of 72 h to obtain whey protein probiotic freeze-dried powder.

[0037] Example 3

[0038] The present embodiment provides a cholesterol-lowering whey protein probiotic freeze-dried powder, which is prepared by the following preparation method, comprising the steps of:

[0039] (1) pasteurizing the whey culture medium at 95°C for 15 min to obtain a whey fermentation medium;

[0040] (2) The activated Lactobacillus paracasei strain was inoculated into whey fermentation medium at a 3% inoculum rate and fermented at 37°C for 10 h;

[0041] (3) The fermented whey was centrifuged at 10,000 r / min for 15 min, and the supernatant was removed to obtain protein sludge.

[0042] (4) Add 1 part of xylitol, 1 part of VC, 1 part of trehalose and 50 parts of water to 10 parts of protein slurry. The mixture is sheared at 10000-12000 rpm for 3 min. After uniform shearing, it is quickly pre-frozen at a temperature of -80℃ for 12 h. After pre-freezing, it is vacuum freeze-dried at a temperature of -50℃, a pressure of 0.5-1 kPa and a time of 72 h to obtain whey protein probiotic freeze-dried powder.

[0043] Scanning electron microscopy images of whey protein probiotic freeze-dried powder with different inoculation amounts of Example 1-3 are as follows Figure 1 As shown in Table 1, as the amount of probiotic inoculation increases, whey protein tends to entrap the bacteria, and this effect is highly correlated with higher bacterial counts. The viable bacterial counts of whey protein probiotic freeze-dried powders with different inoculation amounts in Examples 1-3 are shown in Table 1 after storage for different periods of time:

[0044] Table 1 Viable bacteria count of whey protein probiotic freeze-dried powder (CFU / g)

[0045]

[0046] Comparing the data in Table 1, the probiotic freeze-dried powder prepared in Example 3 has the best stability and the longest storage time, thereby better extending the shelf life of the probiotic freeze-dried powder. The whey protein probiotic freeze-dried powder has the functions of regulating intestinal flora, improving immunity, lowering cholesterol, and improving memory.

[0047] Example 4

[0048] The cholesterol-lowering whey protein probiotic freeze-dried powder prepared in any one of Examples 1-3 is used to prepare a medicine; the medicine contains the cholesterol-lowering whey protein probiotic freeze-dried powder and can assist in lowering cholesterol.

[0049] Example 5

[0050] The cholesterol-lowering whey protein probiotic freeze-dried powder prepared in any one of Examples 1-3 is used to prepare a nutritional product; the nutritional product contains the cholesterol-lowering whey protein probiotic freeze-dried powder and can assist in lowering cholesterol.

[0051] Example 6

[0052] The cholesterol-lowering whey protein probiotic freeze-dried powder prepared in any one of Examples 1-3 is used to prepare a health product; the health product contains the cholesterol-lowering whey protein probiotic freeze-dried powder and can assist in lowering cholesterol.

Claims

1. A method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder, characterized in that: The following steps are involved: (1) pasteurizing the whey culture medium to obtain a whey fermentation medium; (2) adding the activated Lactobacillus paracasei strain into a whey fermentation medium for fermentation; (3) centrifuging the fermented whey and removing the supernatant to obtain protein sludge; (4) Add 0.5-2 parts of xylitol, 0.5-2 parts of VC, 1-2 parts of trehalose, and 50-100 parts of water to 10-20 parts of protein mud. Shear the mixture evenly and pre-freeze it. After pre-freezing, vacuum freeze-dry it to obtain whey protein probiotic freeze-dried powder.

2. The method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder according to claim 1, wherein: In step (1), the pasteurization conditions of the whey culture medium are 90-95°C for 10-15 minutes.

3. The method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder according to claim 1, wherein: In step (2), the inoculation amount of Lactobacillus paracasei is 1-3%, the fermentation time is 10-18 h, and the fermentation temperature is 37-42°C.

4. The method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder according to claim 1, wherein: In step (3), the centrifugation parameters are 8000-12000 r / min, 10-30 min.

5. The method for preparing a cholesterol-lowering whey protein probiotic freeze-dried powder according to claim 1, wherein: In step (4), the shearing parameters are 10000~12000 rpm, 3 min; the prefreezing temperature is -20~-80 ℃, and the prefreezing time is 8~24 h; the vacuum freeze-drying temperature is -50~-80 ℃, the vacuum freeze-drying pressure is 0.5~1 kPa, and the vacuum freeze-drying time is 48~72 h.

6. A whey protein probiotic freeze-dried powder for lowering cholesterol, characterized by: Prepared according to any one of claims 1 to 5.

7. The use of a whey protein probiotic freeze-dried powder for lowering cholesterol according to claim 6, characterized in that: Used for preparing products for lowering cholesterol; the products are one or more of medicines, nutritional products or health products.

8. A product characterized by: The product contains the cholesterol-lowering whey protein probiotic freeze-dried powder according to claim 6; the product is one or more of a medicine, a nutritional product or a health product.

Images