Phalanx polypeptide-containing tincture composition for eliminating erlpain and preparation method of phalanx polypeptide-containing tincture

Abstract

The invention relates to the technical field of medicine, in particular to a phalanx polypeptide-containing tincture formula for relieving pain, diminishing swelling and dispersing blood stasis and a preparation process thereof.The preparation process comprises the following steps: S1, processing 10-20 parts of radix aconiti kusnezoffii, 5-15 parts of radix aconiti kusnezoffii and 5-15 parts of semen strychni respectively, cleaning 10-20 parts of radix clematidis and 10-20 parts of pawpaw, and then crushing the radix clematidis and the pawpaw into coarse powder; s2, percolating the coarse powder by using ethanol as a solvent, and collecting percolate; s3, taking fresh phalanx, adding a proper amount of enzyme to carry out enzymolysis reaction, and then separating and purifying to obtain phalanx polypeptide; s4, mixing the percolation liquid collected in the step S2 with the phalanx polypeptide obtained in the step S3, soaking, concentrating and filtering to obtain a Xiergtongtincture extracting solution; and adding a transdermal enhancer, uniformly stirring, and filtering to remove impurities, thereby obtaining the Xiergpain tincture finished product. When the Xiaergpain tincture prepared by the invention is used for treating, the Xiaergpain tincture is smeared on an affected part for 4-5 times every day, and after treatment for two weeks, the arthralgia of 80% of patients is relieved, the stiff feeling is relieved, and the activity is improved.

Description

Technical Field

[0001] The invention relates to the technical field of medicine, in particular to a Xiaoertong tincture formula containing a toe bone polypeptide for relieving pain, reducing swelling and removing blood stasis, and a preparation process thereof. Background Art

[0002] Pain is a common symptom of various diseases and injuries. There are many analgesics on the market, but external tinctures are still in great demand due to their ease of use and good local effects. Due to the addition of phalangeal polypeptide, the Xiaoertong tincture of the present invention has more significant effects in terms of alleviating pain, reducing swelling and removing blood stasis, and can improve patients' symptoms more quickly. Existing Xiaoertong tincture still has room for improvement in terms of analgesic effect, drug absorption efficiency, and safety. Therefore, developing a new and more effective Xiaoertong tincture prescription is of great significance. Summary of the Invention

[0003] The object of the present invention is to provide a Xiaoertong tincture formula containing phalangeal polypeptide and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0004] To achieve the above object, the present invention provides the following technical solutions: The invention provides a Xiaoertong tincture formula containing phalangeal polypeptide. The raw materials include, by weight, 10-20 parts of raw aconite root, 5-15 parts of raw chuanwu root, 5-15 parts of strychnine seeds, 10-20 parts of clematis root, 10-20 parts of papaya and 50-55 parts of phalangeal polypeptide.

[0005] Furthermore, the formulation further comprises a transdermal enhancer, which is azone, and the amount of the transdermal enhancer is 0.5%-0.6% of the total formulation amount.

[0006] The present invention also provides a method for preparing a Xiaoertong tincture formula containing a phalangeal polypeptide, comprising the following steps: S1. Separately process 10-20 parts of raw Aconitum kusnezoffii, 5-15 parts of raw Chuanwu, and 5-15 parts of Strychnos nux vomica, wash 10-20 parts of Clematidis and 10-20 parts of Papaya, and then grind them together into coarse powder; S2. Using 60-75% ethanol as solvent, percolate the coarse powder at a flow rate of 1-4 ml / min, and collect the percolate until it is 8-11 times the weight of the medicinal material; S3. Take fresh phalanges and add an appropriate amount of enzyme for enzymatic hydrolysis. Control the reaction temperature to 37°C and the reaction time to 5 hours. After the enzymatic hydrolysis is completed, separate and purify the phalange polypeptide using a membrane separation device. S4. The percolation liquid collected in step S2 is mixed with the phalangeal polypeptide obtained in step S3, and the mixture is soaked, concentrated, and filtered to obtain a finished product of the Xiaoertong tincture extract; a transdermal enhancer is then added, stirred evenly, and the ethanol is adjusted to a mass percentage of ethanol in the final formulation of 65-75%, and the impurities are removed by filtration, and the mixture is filled into a brown glass bottle to obtain a finished product of the Xiaoertong tincture.

[0007] Furthermore, in step S3, the enzyme is trypsin, and the mass ratio of the enzyme to the fresh toe bone is 0.2%-0.8%.

[0008] Furthermore, in step S1, the processing methods of the raw Aconitum kusnezoffii and the raw Aconitum carmichaelii include water soaking and steaming, and the processing method of the Strychnos nux vomica includes sand blanching.

[0009] Furthermore, in step S4, the mass ratio of the percolate collected in step S2 to the phalangeal polypeptide obtained in step S3 is 10:1-2.

[0010] Furthermore, in step S4, the mass percentage of the transdermal enhancer to the total amount of the formula is 0.5%-0.6%.

[0011] Compared with the prior art, the present invention has the following beneficial effects: Synergistic Effect: Phalangeal polypeptides promote tissue repair and regeneration, acting synergistically with traditional Chinese medicine ingredients such as Radix Aconiti Kusnezoffii, Radix Chuanwu, Semen Strychnos nux vomica, and Radix Clematis chinensis, which promote blood circulation, resolve stasis, reduce swelling, and relieve pain. This significantly enhances the effectiveness of treating conditions such as traumatic injuries and rheumatic pain. Radix Aconiti Kusnezoffii, Radix Chuanwu, and Semen Strychnos nux vomica in the formula work synergistically through different analgesic mechanisms, while Semen Strychnos nux vomica and papaya aid in improving blood circulation and tissue repair, resulting in a more sustained and pronounced analgesic effect.

[0012] Good transdermal performance: By utilizing the transdermal promoting effect of ethanol and azone, the drug can quickly penetrate the skin and act locally, reducing the occurrence of systemic adverse reactions.

[0013] The Xiaoertong tincture prepared by the present invention is used for treatment, and the affected area is applied 4-5 times a day. After two weeks of treatment, more than 95% of the patients can achieve joint pain relief or even disappearance, reduced stiffness, and improved mobility. DETAILED DESCRIPTION

[0014] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0015] 1. Formula ingredients 1.1 Main medicinal ingredients: In the Xiaoertong tincture formula of the present invention, the toe bone polypeptide has the effect of promoting tissue repair and regeneration, and has a synergistic effect with other Chinese medicinal ingredients in the formula that have the functions of promoting blood circulation, removing blood stasis, reducing swelling and relieving pain, and can significantly improve the effect of treating diseases such as traumatic injuries, rheumatic pain, and the like.

[0016] Raw Aconitum: It has the effects of dispelling wind and dampness, warming the meridians and relieving pain. Its main active ingredient, aconitine and other alkaloids, can block the conduction of nerve impulses, thereby exerting its analgesic effect.

[0017] Raw Chuanwu: Similar to raw Caowu, it has strong analgesic and anti-inflammatory abilities, and the diester alkaloids it contains have a significant effect on relieving pain.

[0018] Strychnine: It can relieve pain, dissipate stagnation and reduce swelling. The strychnine in Strychnine can excite the central nervous system, increase the pain threshold and enhance the analgesic effect.

[0019] Clematis chinensis: It can dispel rheumatism and dredge meridians. The ingredients it contains, such as anemonin, help improve local blood circulation and assist in pain relief.

[0020] Papaya: It relaxes the muscles and tendons, soothes the stomach and eliminates dampness. Oleanolic acid and other ingredients in papaya can fight inflammation and relieve pain, and promote tissue repair.

[0021] 1.2 Auxiliary ingredients: Ethanol: As a solvent, it dissolves the active ingredients in medicinal materials and promotes drug penetration through the skin, enhancing absorption. The dosage is determined based on the total amount of other ingredients and the final product concentration.

[0022] Transdermal enhancer: Azone is selected, which can improve the permeability of the skin, making it easier for drugs to penetrate the skin and reach the lesion site, thereby increasing the bioavailability of drugs.

[0023] 2. Preparation process 2.1 Pretreatment of medicinal materials: Wash raw Radix Aconiti Kusnezoffii, raw Radix Chuanwu, Strychnos nux vomica, Clemati radix, and papaya to remove impurities. Prepare raw Radix Aconiti Kusnezoffii, raw Radix Chuanwu, and Strychnos nux vomica separately to reduce toxicity. For example, raw Radix Aconiti Kusnezoffii and raw Radix Chuanwu can be soaked or steamed, while Strychnos nux vomica can be scalded with sand.

[0024] Phalangeal polypeptide extraction: For phalangeal polypeptides, enzymatic hydrolysis combined with membrane separation technology is used. First, the phalanges are hydrolyzed with a specific enzyme to release the polypeptides. The hydrolysis products are then separated and purified using membrane separation technology to obtain high-purity phalangeal polypeptides.

[0025] 2.2 Extraction of active ingredients: Use the cold extraction method. Grind the pretreated medicinal materials into a coarse powder, place them in a percolation cylinder, and percolate at a constant flow rate using ethanol as the solvent. Collect the percolate and set aside. Add the percolate and phalangeal polypeptide to the ethanol, seal, and soak for 10-20 days, stirring every 2-3 days.

[0026] After the soaking is completed, the mixture is filtered and the filtrate is collected to obtain the intermediate of Xiaoertong tincture.

[0027] 2.3 Add auxiliary ingredients: Add an appropriate amount of azone to the percolated liquid, stir evenly, adjust the ethanol concentration to a specified range, and obtain the finished product of Xiaoertong tincture through the steps of filtering and filling. Example 1

[0028] A method for preparing a xiaoertong tincture containing phalangeal polypeptide comprises the following specific steps: 1.1 Preparation of medicinal materials: Weigh 10g of raw Aconitum kusnezoffii, 5g of raw Chuanwu, 5g of Strychnos nux vomica, 10g of Clematidis, 10g of Papaya, and 50g of phalangeal polypeptide extract.

[0029] 1.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0030] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0031] 1.3 Extraction process: Wash the clematis root and papaya, then grind the processed herbs, clematis root, and papaya into a coarse powder. Place the powder in a percolation cylinder. Use 60% ethanol as the solvent and percolate at a flow rate of 1 ml / min. Collect the percolate until it is 11 times the weight of the herbs.

[0032] Phalangeal polypeptide extraction: 50g of fresh phalanges were enzymatically hydrolyzed with 0.1g of trypsin at 37°C for 5 hours. After enzymatic hydrolysis, the peptides were separated and purified using a membrane separation device to obtain phalangeal polypeptides.

[0033] 1.4 Finished product: Mixing and Refining: The percolate and phalangeal polypeptide are mixed in a 10:1 mass ratio, soaked, concentrated, filtered, and purified to obtain the finished Xiaoertong Tincture extract. Add 0.5% (mass fraction) of azone to the total formula, stir evenly, adjust the ethanol concentration to 65-75%, filter to remove impurities, and fill into brown bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Example 2

[0034] A method for preparing a xiaoertong tincture containing phalangeal polypeptide comprises the following specific steps: 1.1 Preparation of medicinal materials: Weigh 10g of raw Aconitum kusnezoffii, 5g of raw Chuanwu, 5g of Strychnos nux vomica, 10g of Clematidis, 10g of Papaya, and 50g of phalangeal polypeptide extract.

[0035] 1.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0036] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0037] 1.3 Extraction process: Wash the clematis root and papaya, then grind the processed herbs, clematis root, and papaya into a coarse powder. Place the powder in a percolation cylinder. Use 75% ethanol as the solvent and percolate at a flow rate of 4 ml / min. Collect the percolate until it reaches 8 times the weight of the herbs.

[0038] Phalangeal polypeptide extraction: 50g of fresh phalanges were enzymatically hydrolyzed with 0.4g of trypsin at 37°C for 5 hours. After the enzymatic hydrolysis, the peptides were separated and purified using a membrane separation device.

[0039] 1.4 Finished product: Mixing and Refining: The percolate and phalangeal polypeptide are mixed in a mass ratio of 10:2, soaked, concentrated, filtered, and purified to obtain the finished Xiaoertong Tincture extract. Add 0.6% (mass fraction) of azone to the total formula, stir evenly, adjust the ethanol concentration to 65-75%, filter to remove impurities, and fill into brown bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Example 3

[0040] A method for preparing a xiaoertong tincture containing phalangeal polypeptide comprises the following specific steps: 2.1 Preparation of medicinal materials: Weigh 20g of raw Aconitum kusnezoffii, 15g of raw Chuanwu, 15g of Strychnos nux vomica, 20g of Clematidis, 20g of Papaya, and 55g of phalangeal polypeptide extract.

[0041] 2.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0042] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0043] 2.3 Extraction process: Wash the clematis root and papaya. Grind the processed herbs, clematis root, and papaya into a coarse powder and place in a percolation cylinder. Use 60% ethanol as the solvent and percolate at a flow rate of 1 ml / min. Collect the percolation liquid until it is 11 times the weight of the herbs.

[0044] Phalangeal polypeptide extraction: 50g of fresh phalanges were enzymatically hydrolyzed with 0.1g of trypsin at 37°C for 5 hours. After enzymatic hydrolysis, the peptides were separated and purified using a membrane separation device to obtain phalangeal polypeptides.

[0045] 2.4 Finished products: Mixing and Refining: The percolate and phalangeal polypeptide are mixed in a mass ratio of 10:1, soaked, then concentrated, filtered, and purified to obtain the finished Xiaoertong Tincture extract. Add 0.5% (mass fraction) of azone to the total formulation, stir evenly, adjust the ethanol concentration to 65-75%, filter to remove impurities, and fill into brown glass bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Example 4

[0046] A method for preparing a xiaoertong tincture containing phalangeal polypeptide comprises the following specific steps: 2.1 Preparation of medicinal materials: Weigh 20g of raw Aconitum kusnezoffii, 15g of raw Chuanwu, 15g of Strychnos nux vomica, 20g of Clematidis, 20g of Papaya, and 55g of phalangeal polypeptide extract.

[0047] 2.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0048] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0049] 2.3 Extraction process: Wash the clematis root and papaya. Grind the processed herbs, clematis root, and papaya into a coarse powder and place in a percolation cylinder. Use 75% ethanol as the solvent and percolate at a flow rate of 4 ml / min. Collect the percolate until it is 8 times the weight of the herbs.

[0050] Phalangeal polypeptide extraction: 50g of fresh phalanges were enzymatically hydrolyzed with 0.4g of trypsin at 37°C for 5 hours. After the enzymatic hydrolysis, the peptides were separated and purified using a membrane separation device.

[0051] 2.4 Finished products: Mixing and Refining: The percolate and phalangeal polypeptide are mixed in a mass ratio of 10:2, soaked, concentrated, filtered, and purified to obtain the finished Xiaoertong Tincture extract. Add 0.6% (mass fraction) of azone to the total formulation, stir evenly, adjust the ethanol concentration to 65-75%, filter to remove impurities, and fill into brown glass bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Comparative Example 1

[0052] This comparison is a preparation method of Xiaoertong tincture without adding phalangeal polypeptide: 1.1 Preparation of medicinal materials: Weigh 10g of raw Aconitum kusnezoffii, 10g of raw Chuanwu, 5g of Strychnos nux vomica, 15g of Clematidis, and 15g of Papaya.

[0053] 1.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0054] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0055] 1.3 Extraction process: Wash the clematis root and papaya, then grind the processed herbs, clematis root, and papaya into a coarse powder. Place the powder in a percolation cylinder. Use 60% ethanol as the solvent and percolate at a flow rate of 1 ml / min. Collect the percolate until it is 11 times the weight of the herbs.

[0056] 1.4 Finished product: Refining: Through concentration, filtration, and other refining operations, the finished Xiaoertong Tincture extract is obtained. Azone (0.5% by mass) is then added, stirring evenly. The ethanol concentration is adjusted to 65-75%. Filter to remove impurities, and fill into brown bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Comparative Example 2

[0057] This comparison is a preparation method of Xiaoertong tincture without adding phalangeal polypeptide: 1.1 Preparation of medicinal materials: Weigh 10g of raw Aconitum kusnezoffii, 5g of raw Chuanwu, 5g of Strychnos nux vomica, 10g of Clematidis, and 10g of Papaya.

[0058] 1.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0059] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0060] 1.3 Extraction process: Wash the clematis root and papaya, then grind the processed herbs, clematis root, and papaya into a coarse powder. Place the powder in a percolation cylinder. Use 75% ethanol as the solvent and percolate at a flow rate of 4 ml / min. Collect the percolate until it reaches 8 times the weight of the herbs.

[0061] 1.4 Finished product: Refining: Through concentration, filtration, and other refining operations, the finished Xiaoertong Tincture extract is obtained. Azone (0.6% by mass) is then added, stirred evenly, and the ethanol concentration is adjusted to 65-75%. Filter to remove impurities, and fill into brown bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Comparative Example 3

[0062] This comparison is a preparation method of Xiaoertong tincture without adding phalangeal polypeptide: 2.1 Preparation of medicinal materials: Weigh 20g of raw Aconitum kusnezoffii, 15g of raw Chuanwu, 15g of Strychnos nux vomica, 20g of Clematidis, and 20g of papaya.

[0063] 2.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0064] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0065] 2.3 Extraction process: Wash the clematis root and papaya. Grind the processed herbs, clematis root, and papaya into a coarse powder and place in a percolation cylinder. Use 60% ethanol as the solvent and percolate at a flow rate of 1 ml / min. Collect the percolation liquid until it is 11 times the weight of the herbs.

[0066] 2.4 Finished products: Refining: Through concentration, filtration, and other refining operations, the finished Xiaoertong Tincture extract is obtained. Azone (0.5% by mass) is then added, stirring evenly. The ethanol concentration is adjusted to 65-75%. Filter to remove impurities, and fill into brown glass bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture. Comparative Example 4

[0067] This comparison is a preparation method of Xiaoertong tincture without adding phalangeal polypeptide: 2.1 Preparation of medicinal materials: Weigh 20g of raw Aconitum kusnezoffii, 15g of raw Chuanwu, 15g of Strychnos nux vomica, 20g of Clematidis, and 20g of papaya.

[0068] 2.2 Preparation process: Raw Aconitum kusnezoffii and Raw Aconitum carmichaelii: Soak in water for 3 days, changing the water twice a day, then steam for 1.5 hours, take out, slice, dry and set aside.

[0069] Strychnine: Use the sand scalding method to fry the Strychnine with an appropriate amount of river sand until the surface of the Strychnine bulges and the color inside becomes darker. Sieve out the river sand and let it cool for later use.

[0070] 2.3 Extraction process: Wash the clematis root and papaya. Grind the processed herbs, clematis root, and papaya into a coarse powder and place in a percolation cylinder. Use 75% ethanol as the solvent and percolate at a flow rate of 4 ml / min. Collect the percolate until it is 8 times the weight of the herbs.

[0071] 2.4 Finished products: Refining: Through concentration, filtration, and other refining operations, the finished Xiaoertong Tincture extract is obtained. Azone (0.6% by mass) is then added, stirred evenly, and the ethanol concentration is adjusted to 65-75%. Filter to remove impurities, and fill into brown glass bottles (50 ml per bottle) to obtain the finished Xiaoertong Tincture.

[0072] Utilizing the above-mentioned Examples 1-4 and Comparative Examples 1-4 of the present invention, a total of 8 formulations were used to treat 1600 patients with periarthritis of shoulder. The formulations were applied once daily to the affected area in the morning, noon, and evening. After one week of clinical trials, the data were summarized as shown in Table 1 below: Table 1

[0073] Note: Cured - clinical symptoms completely disappear and normal life resumes; Effective - clinical symptoms partially disappear and various signs gradually improve; Ineffective - no significant improvement in symptoms and signs.

[0074] In Example 4, 168 people were cured, 31 of whom were effective, and only one person experienced no effect from the Xiaoertong tincture using this formula. The overall effective rate reached 99.5%, a 21% increase compared to Comparative Example 4 (which used the same formulation parameters except for the omission of the phalangeal polypeptide). This result demonstrates that the addition of the phalangeal polypeptide, in conjunction with raw kusnezoffii, raw chuanwu, nux vomica, and clematis root, which promote blood circulation, resolve stasis, reduce swelling, and alleviate pain, significantly improving the efficacy of treating conditions such as traumatic injuries and rheumatic pain.

[0075] Synchronously, contrasting the above-described embodiments and comparative examples, it is found that after adding phalangeal polypeptide in the embodiments, analgesic and anti-inflammatory effects are all better than the xiaoertong tincture without phalangeal polypeptide.Wherein, analgesic effect is divided into 9 groups through selecting 90 mice to carry out writhing test, 10 in each group, and the 9th group is the untreated group, and the 1st-8th groups are tested by whole body wiping embodiment 1-4 and comparative example 1-4 medicinal liquid respectively.First, white mice are anesthetized with ether, then white mice are fixed on a special instrument, and its limbs are allowed to stretch freely.Then, wipe the corresponding medicinal liquid respectively, observe the number of writhings in 30 minutes afterwards, the results are shown in Table 2.Find that the number of writhings containing phalangeal polypeptide is reduced by 30% than the xiaoertong tincture without phalangeal polypeptide, illustrating that the addition of phalangeal polypeptide can be synergistic with other ingredients in the prescription, and achieves better analgesic effect.

[0076] Table 2

[0077] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, method, article, or apparatus.

[0078] It should be noted that the above content merely illustrates the technical idea of ​​the present invention and cannot be used to limit the scope of protection of the present invention. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications all fall within the scope of protection of the claims of the present invention.

Claims

1. A Xiaoertong tincture formula containing phalangeal polypeptide, characterized in that: The raw material components include, by weight, 10-20 parts of raw aconite root, 5-15 parts of raw chuanwu root, 5-15 parts of strychnine seeds, 10-20 parts of clematis root, 10-20 parts of papaya, and 50-55 parts of phalangeal polypeptide.

2. The Xiaoertong tincture formula containing phalangeal polypeptide according to claim 1, characterized in that: The prescription further comprises a transdermal enhancer, which is azone, and the amount of the transdermal enhancer is 0.5%-0.6% of the total amount of the prescription.

3. A method for preparing a Xiaoertong tincture formula containing phalangeal polypeptide, characterized in that: The following steps are involved: S1. Separately process 10-20 parts of raw Aconitum kusnezoffii, 5-15 parts of raw Chuanwu, and 5-15 parts of Strychnos nux vomica, wash 10-20 parts of Clematidis and 10-20 parts of Papaya, and then grind them together into coarse powder; S2. Using 60-75% ethanol as solvent, percolate the coarse powder at a flow rate of 1-4 ml / min, and collect the percolation liquid to 8-11 times the weight of the medicinal material; S3. Take fresh phalanges and add an appropriate amount of enzyme for enzymatic hydrolysis. Control the reaction temperature to 37°C and the reaction time to 5 hours. After the enzymatic hydrolysis is completed, separate and purify the phalange polypeptide using a membrane separation device. S4. The percolation liquid collected in step S2 is mixed with the phalangeal polypeptide obtained in step S3, and the mixture is soaked, concentrated, and filtered to obtain a finished product of the Xiaoertong tincture extract; a transdermal enhancer is then added, stirred evenly, the ethanol concentration is adjusted to 65-75%, the impurities are removed by filtration, and the mixture is filled into a brown glass bottle to obtain a finished product of the Xiaoertong tincture.

4. The method for preparing the Xiaoertong tincture formula containing phalangeal polypeptide according to claim 3, wherein: In step S3, the enzyme is trypsin, and the mass percentage of the enzyme to the fresh toe bone is 0.2%-0.8%.

5. The method for preparing the Xiaoertong tincture formula containing phalangeal polypeptide according to claim 3, wherein: In step S1, the processing methods of the raw Aconitum kusnezoffii and the raw Aconitum carmichaelii include water soaking and steaming, and the processing method of the Nux vomica includes sand blanching.

6. The method for preparing the Xiaoertong tincture formula containing phalangeal polypeptide according to claim 3, wherein: In step S4, the mass ratio of the permeate collected in step S2 to the phalangeal polypeptide obtained in step S3 is 10:1-2.

7. The method for preparing the Xiaoertong tincture formula containing phalangeal polypeptide according to claim 3, wherein: In step S4, the mass percentage of the transdermal enhancer to the total amount of the formula is 0.5%-0.6%, and the transdermal enhancer is azone.

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