A controllable degradation of siliconized DNA hydrogel and its preparation method and application

By introducing silanization and specific DNA structure assembly into DNA hydrogels, controllable degradation silanized DNA hydrogels are formed, solving the problems of insufficient mechanical properties and uncontrollable degradation of DNA hydrogels. This achieves high mechanical properties and controllable degradation, making it suitable for cell culture and drug release.

CN120590649BActive Publication Date: 2026-06-02SHANDONG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANDONG UNIV
Filing Date
2025-05-30
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing DNA hydrogels suffer from limitations in mechanical properties and uncontrollable degradation, making it difficult to withstand shear and tensile forces in physiological environments. Furthermore, their degradation behavior is difficult to regulate, thus limiting their application in drug delivery and tissue engineering.

Method used

A silica layer is deposited in the DNA network by silicification reaction, which combines the assembly of Y-shaped and linear DNA structures to form a controllable degradation silicified DNA hydrogel. The unpaired single-stranded regions are used as enzyme targets to achieve selective degradation, and the degradation of the gel is controlled by regulating the enzyme concentration and the number of unpaired bases.

Benefits of technology

It significantly improves the mechanical properties and thermal stability of hydrogels, while enabling enzyme-triggered controllable degradation, making it suitable for cell culture and drug release, and enhancing strain tolerance and biocompatibility.

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Abstract

The present application relates to a controllable degradation of siliconized DNA hydrogel and its preparation method and application, the hydrogel is assembled into a three-dimensional network through the DNA structure with single-stranded region in the center and linear DNA structure, and the mechanical properties and thermal stability thereof are improved through in-situ siliconization. The precisely designed single-stranded region can still be recognized and degraded by specific nucleic acid enzyme after siliconization, realizing the accurate control of selective degradation. Taking human umbilical vein endothelial cells as an example, the present application verifies the good biocompatibility of the material, and the present application can be used for cell embedding and release, and is suitable for advanced biological materials, medical, pharmaceutical and other research and application fields such as organoid culture, tissue engineering and controllable drug release.
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