A collection device for extracting environmental DNA from a water body

Abstract

The application relates to the technical field of water sample collection equipment, and discloses a collection equipment for extracting water body environmental DNA, wherein a plurality of assembly openings are arranged on the length direction of the side wall of a mounting cylinder; the output end of a motor is transmissionally matched with a main driving mechanism, the main driving mechanism is transmissionally matched with a plurality of driven mechanisms, the plurality of driven mechanisms are one-to-one corresponding to the plurality of assembly openings, the driven mechanisms are transmissionally matched with first connecting plates, and a plurality of insertion holes are arranged on the first connecting plates; a filter plate is detachably connected to the inner cavity of a sampling cylinder, the sampling cylinder can be detachably connected with the assembly openings, a plurality of opening and closing cylinders are arranged on the side wall of the sampling cylinder, opening and closing mechanisms are arranged in the opening and closing cylinders, a plurality of following mechanisms are transmissionally matched with the plurality of opening and closing mechanisms, the following mechanisms are transmissionally matched with second connecting plates, a plurality of insertion columns are fixedly connected to the second connecting plates, and the insertion columns are inserted into the insertion holes; the application can quickly obtain the DNA information of the water body environment at different depths, the sampling equipment does not need to be repeatedly operated, and the collection efficiency is effectively improved.

Description

Technical Field

[0001] This invention relates to the field of water sample collection equipment technology, and in particular to a collection device for extracting DNA from aquatic environments. Background Technology

[0002] Environmental DNA (eDNA) technology refers to the use of DNA extracted directly from environmental samples such as soil, water, and feces. This DNA may come from biological remains, cells, secretions, excrement, blood, etc.

[0003] When collecting DNA from aquatic environments, it is necessary to use collection equipment to obtain water samples and filter them through a filter plate to obtain the DNA in the water. However, existing collection equipment, such as the Chinese patent with publication number CN205785912U, requires multiple adjustments to the position of the equipment to obtain water samples at different depths. The operation is cumbersome and cannot quickly collect water samples at different depths in layers. Summary of the Invention

[0004] The purpose of this invention is to provide a collection device for extracting DNA from aquatic environments, aiming to solve or improve at least one of the aforementioned technical problems.

[0005] To achieve the above objectives, the present invention provides the following solution: The present invention provides a collection device for extracting DNA from aquatic environments, comprising:

[0006] The mounting cylinder has multiple assembly ports along its length on its side wall.

[0007] A motor is mounted on the mounting cylinder. The output end of the motor is driven by a main drive mechanism. The main drive mechanism is driven by multiple driven mechanisms. Each of the multiple driven mechanisms corresponds to a multiple assembly port. Each driven mechanism is driven by a first connecting plate. The first connecting plate has multiple insertion holes.

[0008] The sampling tube has a filter plate detachably connected to its inner cavity. The sampling tube can be detachably connected to the assembly port. The side wall of the sampling tube is provided with multiple opening and closing tubes. The opening and closing tubes are provided with opening and closing mechanisms. The multiple opening and closing mechanisms are driven and coordinated with a follower mechanism. The follower mechanism is driven and coordinated with a second connecting plate. Multiple insertion posts are fixedly connected to the second connecting plate. The insertion posts are inserted into the insertion holes.

[0009] Optionally, the opening and closing mechanism includes:

[0010] A sealing plug is disposed at the end of the opening and closing cylinder away from the inner cavity of the sampling cylinder. A plurality of first connecting rods are fixedly connected to the sealing plug. A plurality of slots are provided at the end of the opening and closing cylinder. The plurality of slots correspond one-to-one with the plurality of first connecting rods and slide in fit. A first spring is fixedly connected between the end of the slot and the first connecting rod.

[0011] The second connecting rod is fixedly connected to the sealing plug. The end of the second connecting rod away from the sealing plug extends into the inner cavity of the sampling tube and is fixedly connected to a force plate. The force plate is used to cooperate with the follow-up mechanism in transmission.

[0012] Optionally, the follower mechanism includes:

[0013] The first support plate is fixedly connected to the inner wall of the sampling cylinder;

[0014] The first rotating rod is rotatably connected to the first support plate. One end of the first rotating rod extends out of the sampling tube and is fixedly connected to the second receiving plate. A cam is fixedly connected to the first rotating rod, and the cam is used to drive and cooperate with the multiple force plates.

[0015] Optionally, the driven mechanism includes:

[0016] Multiple guide rods are fixedly connected to the inner wall of the mounting cylinder;

[0017] The second support plate is slidably fitted on the multiple guide rods. Multiple second springs are fixedly connected between the second support plate and the inner side wall of the mounting cylinder. A second rotating rod is rotatably connected to the second support plate. One end of the second rotating rod extends into the assembly port and is fixedly connected to the first connecting plate. A short gear is fixedly connected to the end of the second rotating rod away from the first connecting plate.

[0018] The third support plate is fixedly connected to the inner wall of the mounting cylinder. A long gear is rotatably connected to the third support plate. The long gear meshes with the short gear and is in transmission cooperation with the main drive mechanism.

[0019] Optionally, the main drive mechanism includes:

[0020] The third rotating rod is fixedly connected to the output shaft of the motor;

[0021] Multiple first bevel gears are fixedly connected to the third rotating rod;

[0022] Multiple second bevel gears correspond one-to-one with and mesh with multiple first bevel gears, and multiple second bevel gears correspond one-to-one with and are fixedly connected to multiple long gears.

[0023] Optionally, the assembly port is provided with an internal thread groove, and one end of the sampling cylinder is fixedly connected to a first external thread cylinder for threaded engagement with the internal thread groove.

[0024] Optionally, the end of the sampling cylinder away from the first external threaded cylinder is fitted with a first cap via a threaded connection.

[0025] Optionally, an installation ring is fixedly connected to the inner wall of the sampling tube, and an assembly ring is detachably connected to the installation ring by screws, and the filter plate is fixedly connected to the assembly ring.

[0026] Optionally, an electric telescopic rod is fixedly connected to one end of the mounting cylinder.

[0027] Optionally, it also includes a second cap, on which a second external threaded cylinder is fixedly connected, the second external threaded cylinder being used to engage with the internal threaded groove.

[0028] This invention discloses the following technical effects: By setting multiple assembly ports on the mounting cylinder, multiple sampling cylinders can be installed by selecting appropriate assembly ports according to the required sampling depth. The second connecting plate inside the sampling cylinder is connected to the first connecting plate inside the assembly port through a plug-in hole. During sampling, the main drive mechanism is driven by a motor. The main drive mechanism transmits the driving force to the follower mechanism through the driven mechanism and the first and second connecting plates. The follower mechanism controls the opening and closing states of multiple opening and closing mechanisms, allowing water samples to enter the sampling cylinder through the opening and closing cylinder and be filtered by the filter plate to obtain DNA from the water environment. The DNA information of the water environment at different depths can be quickly obtained simply by removing the mounting cylinder from the water environment and disassembling multiple sampling cylinders, without the need for repeated operation of the sampling equipment, effectively improving the collection efficiency. Attached Figure Description

[0029] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. In the drawings:

[0030] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0031] Figure 2 This is a cross-sectional view of the mounting cylinder and sampling cylinder of the present invention;

[0032] Figure 3 This is a cross-sectional view of the opening and closing cylinder of the present invention;

[0033] Figure 4 This is a schematic diagram of the second sealing structure of the present invention;

[0034] Figure 5 This is a schematic diagram of the mounting ring and assembly ring structure of the present invention.

[0035] In the diagram: 1. Mounting cylinder; 2. Assembly port; 3. Motor; 4. First connecting plate; 41. Insertion hole; 5. Sampling cylinder; 6. Filter plate; 7. Opening and closing cylinder; 8. Second connecting plate; 81. Insertion post; 9. Sealing plug; 10. First connecting rod; 11. Slot; 12. First spring; 13. Second connecting rod; 14. Force plate; 15. First support plate; 16. First rotating rod; 17. Cam; 18. Guide rod; 19. Second support plate; 20. Second spring; 21. Second rotating rod; 22. Short gear; 23. Third support plate; 24. Long gear; 25. Third rotating rod; 26. First bevel gear; 27. Second bevel gear; 28. Internal thread groove; 29. ​​First external thread cylinder; 30. First cap; 31. Mounting ring; 32. Assembly ring; 33. Electric telescopic rod; 34. Second cap; 35. Second external thread cylinder. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0038] Reference Figures 1-5 This invention provides a collection device for extracting DNA from aquatic environments, comprising:

[0039] The mounting cylinder 1 has multiple assembly ports 2 along its length on its side wall;

[0040] Motor 3 is mounted on mounting cylinder 1. The output end of motor 3 is driven by a main drive mechanism. The main drive mechanism is driven by multiple driven mechanisms. The multiple driven mechanisms correspond one-to-one with multiple assembly ports 2. The driven mechanisms are driven by a first connecting plate 4. The first connecting plate 4 has multiple insertion holes 41.

[0041] The sampling tube 5 has a filter plate 6 detachably connected to its inner cavity. The sampling tube 5 can be detachably connected to the assembly port 2. The side wall of the sampling tube 5 is provided with multiple opening and closing tubes 7. The opening and closing tubes 7 are provided with opening and closing mechanisms. The multiple opening and closing mechanisms are driven and coordinated with a follower mechanism. The follower mechanism is driven and coordinated with a second connecting plate 8. Multiple insertion posts 81 are fixedly connected to the second connecting plate 8. The insertion posts 81 are inserted into the insertion holes 41.

[0042] By setting multiple assembly ports 2 on the installation cylinder 1, multiple sampling cylinders 5 can be installed by selecting the appropriate position of the assembly port 2 according to the required sampling depth. The second connecting plate 8 inside the sampling cylinder 5 is connected to the first connecting plate 4 inside the assembly port 2 through the insertion post 81 and the insertion hole 41. During sampling, the main drive mechanism is driven by the motor 3. The main drive mechanism transmits the driving force to the follower mechanism through the driven mechanism and the first connecting plate 4 and the second connecting plate 8. The follower mechanism controls the opening and closing state of multiple opening and closing mechanisms, so that the water sample can enter the sampling cylinder 5 through the opening and closing cylinder 7 and be filtered by the filter plate 6 to obtain the DNA of the water environment. The DNA information of the water environment at different depths can be quickly obtained by simply removing the installation cylinder 1 from the water environment and disassembling the multiple sampling cylinders 5. There is no need to repeatedly operate the sampling equipment, which effectively improves the collection efficiency.

[0043] By linking multiple driven mechanisms with a main drive mechanism, vertical profile DNA data can be acquired in a single drop.

[0044] In one embodiment of the present invention, the opening and closing mechanism includes:

[0045] A sealing plug 9 is located at the end of the opening and closing cylinder 7 away from the inner cavity of the sampling cylinder 5. Multiple first connecting rods 10 are fixedly connected to the sealing plug 9. Multiple slots 11 are opened at the end of the opening and closing cylinder 7. The multiple slots 11 correspond one-to-one with the multiple first connecting rods 10 and slide in fit. A first spring 12 is fixedly connected between the end of the slot 11 and the first connecting rod 10.

[0046] The second connecting rod 13 is fixedly connected to the sealing plug 9. The end of the second connecting rod 13 away from the sealing plug 9 extends into the inner cavity of the sampling tube 5 and is fixedly connected to the force plate 14. The force plate 14 is used to cooperate with the follow-up mechanism for transmission.

[0047] In one embodiment of the present invention, the follower mechanism includes:

[0048] The first support plate 15 is fixedly connected to the inner wall of the sampling cylinder 5;

[0049] The first rotating rod 16 is rotatably connected to the first support plate 15. One end of the first rotating rod 16 extends out of the sampling tube 5 and is fixedly connected to the second receiving plate 8. A cam 17 is fixedly connected to the first rotating rod 16. The cam 17 is used to drive and cooperate with multiple force plates 14.

[0050] After the second connecting plate 8 is connected to the first connecting plate 4, when the first connecting plate 4 rotates, the second connecting plate 8 drives the first rotating rod 16 to rotate synchronously. The first rotating rod 16 drives the cam 17 to rotate, so that the cam 17 can squeeze the force plate 14 to move upward. The force plate 14 pushes the second connecting rod 13 to drive the sealing plug 9 to move upward, so that the opening of the opening and closing cylinder 7 is opened, allowing the water sample to enter the sampling cylinder 5 through the opening and closing cylinder 7. After the cam 17 separates from the force plate 14, the first spring 12 and the first connecting rod 10 can make the sealing plug 9 automatically reset and seal the opening and closing cylinder 7.

[0051] By rotatably connecting the first rotating rod 16 to the first support plate 15, when the sampling tube 5 is screwed into the assembly port 2, the positions of the insertion post 81 and the insertion hole 41 are correspondingly inserted, which can prevent the first connecting plate 4 from rotating when the sampling tube 5 is rotated.

[0052] Furthermore, the second receiving plate 8 is provided with a marker to facilitate obtaining the state of the cam 17 inside the sampling tube 5, and the first receiving plate 4 is connected when the marker is at its highest position.

[0053] In one embodiment of the present invention, the driven mechanism includes:

[0054] Multiple guide rods 18 are fixedly connected to the inner wall of the mounting cylinder 1;

[0055] The second support plate 19 is slidably fitted on multiple guide rods 18. Multiple second springs 20 are fixedly connected between the second support plate 19 and the inner wall of the mounting cylinder 1. A second rotating rod 21 is rotatably connected to the second support plate 19. One end of the second rotating rod 21 extends into the assembly port 2 and is fixedly connected to the first connecting plate 4. A short gear 22 is fixedly connected to the end of the second rotating rod 21 away from the first connecting plate 4.

[0056] The third support plate 23 is fixedly connected to the inner wall of the mounting cylinder 1. A long gear 24 is rotatably connected to the third support plate 23. The long gear 24 meshes with the short gear 22 and is in transmission cooperation with the main drive mechanism.

[0057] When the sampling tube 5 is screwed into the assembly port 2, the second connecting plate 8 pushes the first connecting plate 4, so that the first connecting plate 4 pushes the second support plate 19 to slide along multiple guide rods 18 through the second rotating rod 21. With this arrangement, the second connecting plate 8 can be placed outside the first external threaded cylinder 29, which facilitates the insertion and connection of the second connecting plate 8 and the first connecting plate 4. Furthermore, when the second rotating rod 21 slides, the transmission effect of the second rotating rod 21 can be maintained through the cooperation of the short gear 22 and the long gear 24.

[0058] In one embodiment of the present invention, the main drive mechanism includes:

[0059] The third rotating rod 25 is fixedly connected to the output shaft of the motor 3;

[0060] Multiple first bevel gears 26 are fixedly connected to the third rotating rod 25;

[0061] Multiple second bevel gears 27 correspond one-to-one with multiple first bevel gears 26 and mesh with each other, and multiple second bevel gears 27 correspond one-to-one with multiple long gears 24 and are fixedly connected.

[0062] The third rotating rod 25 is driven to rotate by the motor 3. The long gear 24 is rotated by the cooperation of the first bevel gear 26 and the second bevel gear 27. In turn, the long gear 24 drives the short gear 22 to rotate, which in turn makes the second rotating rod 21 rotate.

[0063] In one embodiment of the present invention, an internal threaded groove 28 is provided on the assembly port 2, and a first external threaded cylinder 29 for threaded engagement with the internal threaded groove 28 is fixedly connected to one end of the sampling cylinder 5.

[0064] Each sampling cylinder 5 is connected to the internal thread groove 28 via a first external threaded cylinder 29, and together with the sealing plug 9 and spring reset c, ensures that there is no cross-contamination during the sampling process.

[0065] In one embodiment of the present invention, the end of the sampling cylinder 5 away from the first external threaded cylinder 29 is threadedly fitted with a first cap 30, and the filter plate 6 is located between the plurality of opening and closing cylinders 7 and the first cap 30.

[0066] After water sampling is completed, the sampling tube 5 can be disassembled, the first cap 30 can be removed, and the water can be poured out. During the process of water passing through the filter plate 6, the DNA information in the water environment is intercepted on the filter plate 6.

[0067] In one embodiment of the present invention, an installation ring 31 is fixedly connected to the inner wall of the sampling tube 5, and an assembly ring 32 is detachably connected to the installation ring 31 by screws, and the filter plate 6 is fixedly connected to the assembly ring 32.

[0068] The filter plate 6 can be disassembled and replaced by removing the assembly ring 32 and the mounting ring 31, making the filter plate 6 removable and easy for operators to obtain DNA information of the water environment on the filter plate 6.

[0069] In one embodiment of the present invention, an electric telescopic rod 33 is fixedly connected to one end of the mounting cylinder 1.

[0070] The electric telescopic rod 33 can adjust the position of the mounting cylinder 1 in real time according to the water depth, making it suitable for sampling in deep water areas (such as the oxygen-deficient bottom area of ​​lakes).

[0071] In one embodiment of the present invention, a second cover 34 is further included, on which a second external threaded cylinder 35 is fixedly connected, the second external threaded cylinder 35 being used to engage with the internal threaded groove 28.

[0072] The assembly port 2 without the sampling tube 5 installed can be connected to the internal thread groove 28 through the second external threaded tube 35, so that the second cover 34 can block the assembly port 2 and prevent water seepage inside the equipment from interfering with the sampling results.

[0073] The invented filter plate 6 uses a polycarbonate membrane with a pore size of 0.2-0.45 μm, specifically designed to capture free DNA and microbial cell debris in water. This pore size range can effectively intercept DNA fragments (typically 0.2-2 μm) released by bacteria (0.5-5 μm) and eukaryotic cells (>2 μm), while preventing large particles from clogging the filter membrane.

[0074] This invention is particularly suitable for scenarios requiring the acquisition of environmental DNA from vertical water profiles, such as ecological monitoring of lakes, reservoirs, or oceans. In lake vertical profile sampling, water at different depths may contain specific microbial communities or fish DNA. Multiple sampling tubes (e.g., at depths of 0.5m, 2m, and 5m) can be deployed simultaneously via multiple mounting ports on the mounting tube, achieving simultaneous multi-depth sampling in a single operation. Traditional methods (such as Nissen water samplers or manual stratified samplers) require layer-by-layer operation, with each sampling session taking approximately 10-15 minutes per layer, requiring 30-45 minutes to complete three layers of sampling. In contrast, this invention, through a motor-driven opening and closing mechanism, can complete simultaneous multi-depth sampling within 5 minutes, improving efficiency by 6-9 times and avoiding repeated equipment deployment.

[0075] In the description of this invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this invention, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this invention.

[0076] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A collection device for extracting DNA from aquatic environments, characterized in that, include: The mounting cylinder (1) has multiple assembly ports (2) along its length on its side wall; The motor (3) is mounted on the mounting cylinder (1). The output end of the motor (3) is driven by a main drive mechanism. The main drive mechanism is driven by multiple driven mechanisms. The multiple driven mechanisms correspond one-to-one with the multiple assembly ports (2). The driven mechanisms are driven by a first connecting plate (4). The first connecting plate (4) has multiple insertion holes (41). The sampling tube (5) has a filter plate (6) detachably connected to its inner cavity. The sampling tube (5) can be detachably connected to the assembly port (2). The side wall of the sampling tube (5) is provided with multiple opening and closing tubes (7). The opening and closing tubes (7) are provided with opening and closing mechanisms. The multiple opening and closing mechanisms are driven and cooperated with follower mechanisms. The follower mechanisms are driven and cooperated with a second connecting plate (8). Multiple insertion posts (81) are fixedly connected to the second connecting plate (8). The insertion posts (81) are inserted into the insertion hole (41). The opening and closing mechanism includes: A sealing plug (9) is disposed at the end of the opening and closing cylinder (7) away from the inner cavity of the sampling cylinder (5). A plurality of first connecting rods (10) are fixedly connected to the sealing plug (9). A plurality of slots (11) are provided at the end of the opening and closing cylinder (7). The plurality of slots (11) correspond one-to-one with the plurality of first connecting rods (10) and slide in cooperation. A first spring (12) is fixedly connected between the end of the slot (11) and the first connecting rod (10). The second connecting rod (13) is fixedly connected to the sealing plug (9). The end of the second connecting rod (13) away from the sealing plug (9) extends into the inner cavity of the sampling tube (5) and is fixedly connected to a force plate (14). The force plate (14) is used to cooperate with the follower mechanism in transmission.

2. The collection device for extracting DNA from aquatic environments according to claim 1, characterized in that, The follower mechanism includes: The first support plate (15) is fixedly connected to the inner wall of the sampling cylinder (5); The first rotating rod (16) is rotatably connected to the first support plate (15). One end of the first rotating rod (16) extends out of the sampling tube (5) and is fixedly connected to the second receiving plate (8). A cam (17) is fixedly connected to the first rotating rod (16). The cam (17) is used to drive and cooperate with multiple force plates (14).

3. The collection device for extracting DNA from aquatic environments according to claim 1, characterized in that, The driven mechanism includes: Multiple guide rods (18) are fixedly connected to the inner wall of the mounting cylinder (1); The second support plate (19) is slidably fitted on the multiple guide rods (18). Multiple second springs (20) are fixedly connected between the second support plate (19) and the inner wall of the mounting cylinder (1). A second rotating rod (21) is rotatably connected to the second support plate (19). One end of the second rotating rod (21) extends into the assembly port (2) and is fixedly connected to the first connecting plate (4). A short gear (22) is fixedly connected to the end of the second rotating rod (21) away from the first connecting plate (4). The third support plate (23) is fixedly connected to the inner wall of the mounting cylinder (1). A long gear (24) is rotatably connected to the third support plate (23). The long gear (24) meshes with the short gear (22). The long gear (24) is in transmission cooperation with the main drive mechanism.

4. The collection device for extracting DNA from aquatic environments according to claim 3, characterized in that, The main drive mechanism includes: The third rotating rod (25) is fixedly connected to the output shaft of the motor (3); Multiple first bevel gears (26) are fixedly connected to the third rotating rod (25); Multiple second bevel gears (27) correspond one-to-one with multiple first bevel gears (26) and mesh with each other, and multiple second bevel gears (27) correspond one-to-one with multiple long gears (24) and are fixedly connected.

5. The collection device for extracting DNA from aquatic environments according to claim 1, characterized in that, The assembly port (2) is provided with an internal thread groove (28), and one end of the sampling cylinder (5) is fixedly connected to a first external thread cylinder (29) for threaded engagement with the internal thread groove (28).

6. The collection device for extracting DNA from aquatic environments according to claim 5, characterized in that, The sampling tube (5) has a first cap (30) threadedly fitted to the end away from the first external threaded tube (29).

7. The collection device for extracting DNA from aquatic environments according to claim 1, characterized in that, An installation ring (31) is fixedly connected to the inner wall of the sampling tube (5), and an assembly ring (32) is detachably connected to the installation ring (31) by screws. The filter plate (6) is fixedly connected to the assembly ring (32).

8. The collection device for extracting DNA from aquatic environments according to claim 1, characterized in that, An electric telescopic rod (33) is fixedly connected to one end of the mounting cylinder (1).

9. A collection device for extracting DNA from aquatic environments according to claim 5, characterized in that, It also includes a second cover (34), on which a second external threaded cylinder (35) is fixedly connected, and the second external threaded cylinder (35) is used to engage with the internal threaded groove (28).

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