Oscillator for cell culture medium

Abstract

The invention discloses an oscillator for a cell culture medium, and relates to the technical field of cell culture, in particular to the oscillator for the cell culture medium, which comprises a shell, a first fixed cylinder is fixedly mounted in the shell, and an inner gear is fixedly mounted at the top of the inner wall of the first fixed cylinder; a motor is installed in the first fixing cylinder, a transmission plate is installed at the output end of the motor, a first connecting bearing is installed outside the transmission plate, a fourth connecting bearing is installed on the right side in the transmission plate, a rotating cylinder is installed in the fourth connecting bearing, and a telescopic column penetrates through the interior of the rotating cylinder; and a mounting barrel is mounted above the telescopic column. According to the oscillator for the cell culture medium, circular motion, autorotation motion and up-down reciprocating motion are combined to form multi-dimensional synergistic composite oscillation, three-dimensional diffusion can be achieved through multi-dimensional displacement, the oscillation efficiency of the cell culture medium is improved, and meanwhile the effect is better during oscillation of a large culture volume.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to an oscillator for cell culture medium. Background Art

[0002] Cell culture medium is not only the basic substance that provides cell nutrition and promotes cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. The oscillator in the cell culture medium mainly promotes the uniform mixing of culture medium and cells through continuous mechanical vibration, prevents cell sedimentation or aggregation, thereby ensuring that nutrients and oxygen are fully diffused in the culture medium and maintains the stable environment required for cell metabolism. In addition, oscillation can also reduce the accumulation of local metabolic waste (such as lactic acid), avoid drastic fluctuations in pH, and increase dissolved oxygen levels by enhancing gas-liquid exchange. It is especially suitable for scenarios that require a dynamic environment, such as suspended cell culture or microbial fermentation.

[0003] Existing oscillators usually use motors to drive eccentric wheels, belts or crank-connecting rod mechanisms to convert rotational motion into horizontal or vertical oscillations. The motion mode is usually circular motion or linear swing. Single circular motion only provides mixing within a two-dimensional plane, which is difficult to completely break the stratification of the culture medium, and the cells or particles at the bottom are easy to settle; reciprocating swinging may form a "backflow dead corner" due to its fixed direction, resulting in uneven local nutrient concentration, which will affect the oscillation efficiency of the oscillator. At the same time, when the single motion is used to expand the culture volume (such as in a bioreactor), the mixing efficiency decreases nonlinearly. Summary of the Invention

[0004] In response to the deficiencies of the prior art, the present invention provides an oscillator for cell culture medium, which solves the problem raised in the above background technology that the existing oscillators usually use a motor to drive an eccentric wheel, a belt or a crank-connecting rod mechanism to convert rotational motion into horizontal or vertical oscillation. The motion mode is usually circular motion or linear swing. Single circular motion only provides mixing within a two-dimensional plane, which is difficult to completely break the culture medium stratification phenomenon, and the cells or particles at the bottom are easy to settle; the reciprocating swing may form a "backflow dead corner" due to the fixed direction, resulting in uneven local nutrient concentration. At the same time, when the culture volume is enlarged (such as in a bioreactor), the mixing efficiency decreases nonlinearly.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: an oscillator for cell culture medium, comprising:

[0006] The housing comprises a first fixed cylinder fixedly installed inside the housing, an internal gear fixedly installed on the top of the inner wall of the first fixed cylinder, a motor installed inside the first fixed cylinder, a transmission plate installed on the output end of the motor, a first connecting bearing installed outside the transmission plate, a fourth connecting bearing installed on the right side of the interior of the transmission plate, a rotating cylinder installed inside the fourth connecting bearing, a telescopic column passing through the interior of the rotating cylinder, a mounting barrel installed above the telescopic column, a fixed column fixedly installed on the left side above the transmission plate, a second connecting bearing installed inside the fixed column, a second bevel gear installed on the right side of the fixed column, a first bevel gear installed below the second bevel gear, a transmission gear installed below the first bevel gear, a third connecting bearing installed inside the second bevel gear, a transmission rod installed on the right side of the second bevel gear, a bearing seat installed above the transmission rod, a connecting annular plate installed above the bearing seat, a second fixed cylinder installed above the connecting annular plate, a fifth connecting bearing installed inside the second fixed cylinder, and a connecting cylinder installed inside the fifth connecting bearing.

[0007] As a preferred oscillator for cell culture medium of the present invention, the transmission plate is movably connected to the first fixed cylinder via the first connecting bearing, and the transmission plate is movably connected to the rotating cylinder via the fourth connecting bearing.

[0008] As a preferred oscillator for cell culture medium of the present invention, the transmission gear is fixedly connected to the rotating cylinder, the transmission gear is meshed with the internal gear, and the diameter of the transmission gear is smaller than the diameter of the internal gear.

[0009] As a preferred oscillator for cell culture medium of the present invention, the second bevel gear is movably connected to the fixed column through the second connecting bearing, the first bevel gear is fixedly connected to the rotating cylinder, and the second bevel gear is meshedly connected to the first bevel gear.

[0010] As a preferred oscillator for cell culture medium of the present invention, the transmission rod is movably connected to the second bevel gear via the third connecting bearing, and the transmission rod is movably connected to the connecting annular plate via the bearing seat.

[0011] As a preferred oscillator for cell culture medium of the present invention, the second fixed cylinder is fixedly connected to the mounting barrel, the second fixed cylinder is movably connected to the connecting cylinder through the fifth connecting bearing, and the connecting cylinder is fixedly connected to the connecting annular plate.

[0012] As a preferred oscillator for cell culture medium of the present invention, the rotating cylinder is further provided with:

[0013] A position-limiting movable groove is provided on the inner wall of the rotating cylinder, and a position-limiting movable block is movably installed inside the position-limiting movable groove.

[0014] As a preferred oscillator for cell culture medium of the present invention, the limiting movable block is fixedly connected to the telescopic column, the shape and size of the limiting movable block are consistent with the shape and size of the inside of the limiting movable groove, and the telescopic column is movably connected to the rotating cylinder through the limiting movable groove and the limiting movable block.

[0015] As a preferred oscillator for cell culture medium of the present invention, the mounting barrel is further provided with:

[0016] Rubber bands are installed inside the installation barrel, and the rubber bands are evenly distributed along the interior of the installation barrel.

[0017] As a preferred oscillator for cell culture medium of the present invention, the housing is further provided with:

[0018] A control panel is installed at the front end of the housing, and the control panel is electrically connected to the motor.

[0019] The present invention provides an oscillator for cell culture medium, which has the following beneficial effects:

[0020] 1. This oscillator for cell culture medium is equipped with an internal gear and a transmission gear to drive the rotating cylinder and the rotating telescopic column to rotate, thereby driving the installation barrel to rotate, and then driving the test tube inside the installation barrel to rotate, so that the cell culture medium inside the test tube generates centrifugal force, effectively counteracting the gravitational sedimentation of cells and microcarriers, maintaining the uniform suspension of the high-density culture system, and accelerating the diffusion of metabolic waste. The centrifugal force generated by the rotation cooperates with the revolution of the transmission plate to form an oscillating force, making the shear force distribution more balanced and avoiding local high shear damage, which is particularly beneficial for the three-dimensional culture of stem cell spheres and organoids.

[0021] 2. The oscillator for cell culture medium is equipped with a second bevel gear that cooperates with the transmission rod to push the mounting barrel up and down in a small range. The vertical acceleration directly counteracts the gravity sedimentation, achieving uniform suspension of high-density cells and eliminating the nutrient concentration gradient at the bottom of the culture container.

[0022] 3. The oscillator for cell culture medium combines circular motion, rotational motion and up and down reciprocating movement to form a multi-dimensional synergistic compound oscillation. The multi-dimensional displacement can achieve three-dimensional diffusion, improve the oscillation efficiency of the cell culture medium, and the effect is better when the oscillation is in a larger culture volume. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the main structure of the present invention;

[0024] Figure 2 Schematic diagram of the internal structure of the housing of the present invention;

[0025] Figure 3 For the present invention Figure 2 A schematic diagram of the partially enlarged structure at center A;

[0026] Figure 4 This is a schematic diagram of the connection structure between the telescopic column and the rotating cylinder of the present invention;

[0027] Figure 5 This is a schematic diagram of the connection structure between the internal gear and the transmission gear of the present invention;

[0028] Figure 6 This is a schematic diagram of the connection structure of the connecting cylinder and the connecting annular plate of the present invention;

[0029] Figure 7 This is a schematic diagram of the connection structure between the transmission rod and the second bevel gear of the present invention;

[0030] Figure 8 It is a schematic diagram of the connection structure between the rotating drum and the transmission plate of the present invention.

[0031] In the figure: 1. Shell; 2. Mounting barrel; 3. Fixed column; 4. Internal gear; 5. First fixed cylinder; 6. Motor; 7. First connecting bearing; 8. Transmission gear; 9. First bevel gear; 10. Connecting cylinder; 11. Telescopic column; 12. Second fixed cylinder; 13. Transmission rod; 14. Second bevel gear; 15. Rotating cylinder; 16. Connecting annular plate; 17. Limiting movable groove; 18. Limiting movable block; 19. Transmission plate; 20. Second connecting bearing; 21. Third connecting bearing; 22. Fourth connecting bearing; 23. Bearing seat; 24. Rubber band; 25. Fifth connecting bearing; 26. Control panel. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0033] In the description of the present invention, unless otherwise specified, "plurality" means two or more; terms such as "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," and "tail" indicate positions or relationships based on those shown in the accompanying drawings. These terms are intended solely to facilitate the description of the present invention and simplify the description. They do not indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limiting the present invention. Furthermore, terms such as "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0034] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "connected" and "connection" should be understood in a broad sense. For example, they can refer to fixed connection, detachable connection, or integral connection; mechanical connection, electrical connection; direct connection, or indirect connection through an intermediary. Those skilled in the art will understand the specific meanings of the above terms in the present invention based on the specific circumstances.

[0035] See also Figure 1-8 An oscillator for cell culture medium includes a housing 1, a first fixed cylinder 5 is fixedly installed inside the housing 1, an internal gear 4 is fixedly installed on the top of the inner wall of the first fixed cylinder 5, a motor 6 is installed inside the first fixed cylinder 5, a transmission plate 19 is installed on the output end of the motor 6, a first connecting bearing 7 is installed on the outside of the transmission plate 19, a fourth connecting bearing 22 is installed on the right side of the inside of the transmission plate 19, a rotating cylinder 15 is installed inside the fourth connecting bearing 22, a telescopic column 11 is penetrated inside the rotating cylinder 15, a mounting barrel 2 is installed above the telescopic column 11, a fixed column 3 is fixedly installed on the left side above the transmission plate 19, and the inside of the fixed column 3 A second connecting bearing 20 is installed, a second bevel gear 14 is installed on the right side of the fixed column 3, a first bevel gear 9 is installed below the second bevel gear 14, a transmission gear 8 is installed below the first bevel gear 9, a third connecting bearing 21 is installed inside the second bevel gear 14, a transmission rod 13 is installed on the right side of the second bevel gear 14, a bearing seat 23 is installed above the transmission rod 13, a connecting annular plate 16 is installed above the bearing seat 23, a second fixed cylinder 12 is installed above the connecting annular plate 16, a fifth connecting bearing 25 is installed inside the second fixed cylinder 12, and a connecting cylinder 10 is installed inside the fifth connecting bearing 25.

[0036] In this embodiment, the oscillator for cell culture medium is provided with an internal gear 4 and a transmission gear 8 to drive the rotating cylinder 15 and the rotating telescopic column 11 to rotate, thereby driving the mounting barrel 2 to rotate, and further driving the test tube inside the mounting barrel 2 to rotate, so that the cell culture medium inside the test tube generates centrifugal force, effectively counteracting the gravitational sedimentation of cells and microcarriers, maintaining a uniform suspension of the high-density culture system, and accelerating the diffusion of metabolic waste; the centrifugal force generated by the rotation cooperates with the revolution of the transmission plate 19 to form an oscillating force, making the shear force distribution more balanced, avoiding local high shear damage, and being particularly beneficial for the three-dimensional culture of stem cell spheres and organoids; and a second bevel gear 14 is provided to cooperate with the transmission rod 13 to push the mounting barrel 2 up and down in a small range. The vertical acceleration directly counteracts gravitational sedimentation, achieves a uniform suspension of high-density cells, and eliminates the nutrient concentration gradient at the bottom of the culture container; finally, the oscillator for cell culture medium combines circular motion, rotational motion, and up and down reciprocating motion to form a multi-dimensional synergistic composite oscillation. The multi-dimensional displacement can achieve three-dimensional diffusion, improve the oscillation efficiency of the cell culture medium, and at the same time, the effect is better when oscillating a larger culture volume.

[0037] Going further:

[0038] In an optional embodiment, the transmission plate 19 is movably connected to the first fixed cylinder 5 via the first connecting bearing 7 , and the transmission plate 19 is movably connected to the rotating cylinder 15 via the fourth connecting bearing 22 .

[0039] In this embodiment, when the transmission plate 19 rotates, it can drive the rotating cylinder 15 to perform a circular motion, and further drive the mounting barrel 2 to perform a circular motion.

[0040] Going further:

[0041] In an optional embodiment, the transmission gear 8 is fixedly connected to the rotating cylinder 15 , the transmission gear 8 is meshed with the internal gear 4 , and the diameter of the transmission gear 8 is smaller than the diameter of the internal gear 4 .

[0042] In this embodiment, when the rotating drum 15 performs a circular motion, the transmission gear 8 and the internal gear 4 can drive the rotating drum 15 to rotate.

[0043] Going further:

[0044] In an optional embodiment, the second bevel gear 14 is movably connected to the fixed column 3 through the second connecting bearing 20, the first bevel gear 9 is fixedly connected to the rotating cylinder 15, and the second bevel gear 14 is meshedly connected to the first bevel gear 9.

[0045] In this embodiment, when the rotating drum 15 rotates, the second bevel gear 14 can be driven to rotate by the first bevel gear 9 .

[0046] Going further:

[0047] In an optional embodiment, the transmission rod 13 is movably connected to the second bevel gear 14 via a third connecting bearing 21 , and the transmission rod 13 is movably connected to the connecting annular plate 16 via a bearing seat 23 .

[0048] In this embodiment, when the second bevel gear 14 rotates, it can push the mounting barrel 2 to perform up and down reciprocating motion.

[0049] Going further:

[0050] In an optional embodiment, the second fixed cylinder 12 is fixedly connected to the mounting barrel 2 , the second fixed cylinder 12 is movably connected to the connecting cylinder 10 via a fifth connecting bearing 25 , and the connecting cylinder 10 is fixedly connected to the connecting annular plate 16 .

[0051] In this embodiment, when the mounting barrel 2 rotates, the second fixing barrel 12 and the fifth connecting bearing 25 prevent the connecting barrel 10 and the connecting annular plate 16 from rotating, thereby always ensuring power transmission between the transmission rod 13 and the mounting barrel 2.

[0052] Going further:

[0053] In an optional embodiment, the rotating drum 15 is further provided with:

[0054] The limiting movable groove 17 is provided on the inner wall of the rotating cylinder 15, and a limiting movable block 18 is movably installed inside the limiting movable groove 17; the limiting movable block 18 is fixedly connected to the telescopic column 11, and the shape and size of the limiting movable block 18 are consistent with the shape and size of the inside of the limiting movable groove 17. The telescopic column 11 is movably connected to the rotating cylinder 15 through the limiting movable groove 17 and the limiting movable block 18.

[0055] In this embodiment, when the rotating drum 15 rotates, the telescopic column 11 can be driven to rotate through the limiting movable groove 17 and the limiting movable block 18, thereby driving the mounting barrel 2 to rotate.

[0056] Going further:

[0057] In an optional embodiment, the installation barrel 2 is further provided with:

[0058] The rubber bands 24 are installed inside the installation barrel 2 , and the rubber bands 24 are evenly distributed along the interior of the installation barrel 2 .

[0059] In this embodiment, when a test tube or culture bottle is placed in the mounting barrel 2 , the rubber band 24 will deform because the test tube or culture bottle is larger than the distance between the rubber bands 24 . The elastic force generated by the deformation will bind the test tube or culture bottle inside the mounting barrel 2 .

[0060] Going further:

[0061] In an optional embodiment, the housing 1 is further provided with:

[0062] The control panel 26 is mounted on the front end of the housing 1 , and is electrically connected to the motor 6 .

[0063] In this embodiment, the start and stop of the motor 6 and the rotation speed of the motor 6 can be controlled by the control panel 26 to meet the oscillation requirements.

[0064] In summary, when using the oscillator for cell culture medium, first put the test tube or culture bottle containing the cell culture medium into the interior of the mounting barrel 2, use the deformation elastic force of the rubber band 24 inside the mounting barrel 2 to restrain it, and then use the control panel 26 (model: IKA MS1) controls the motor 6 to be energized and drives the transmission plate 19 to rotate. Since the rotating cylinder 15 is installed on a side deviated from the center of the transmission plate 19, the rotating transmission plate 19 drives the mounting barrel 2 to perform circular motion through the rotating cylinder 15 and the telescopic column 11. Secondly, during the circular rotation of the rotating cylinder 15, the transmission gear 8 rotates under the action of the internal gear 4. While the transmission gear 8 rotates, it drives the rotating cylinder 15 and the telescopic column 11 to rotate, thereby driving the mounting barrel 2 to rotate. Thirdly, when the rotating cylinder 15 rotates, it drives the first bevel gear 9 to rotate, and the first bevel gear 9 drives the second bevel gear 14 to rotate. During the rotation of the second bevel gear 14, the connecting annular plate 16 is pushed up and down through the transmission rod 13. The connecting annular plate 16 then pushes the mounting barrel 2 up and down through the second fixed cylinder 12, the fifth connecting bearing 25 and the connecting cylinder 10, thereby driving the test tube or culture bottle to perform circular motion and up and down movement, thereby achieving composite oscillation of the cell culture medium.

[0065] The above are only preferred specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with this technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solutions and inventive concepts of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. An oscillator for cell culture medium, characterized in that include: A housing (1) is provided, wherein a first fixed cylinder (5) is fixedly installed inside the housing (1), an internal gear (4) is fixedly installed on the top of the inner wall of the first fixed cylinder (5), a motor (6) is installed inside the first fixed cylinder (5), a transmission plate (19) is installed on the output end of the motor (6), a first connecting bearing (7) is installed outside the transmission plate (19), a fourth connecting bearing (22) is installed on the right side inside the transmission plate (19), a rotating cylinder (15) is installed inside the fourth connecting bearing (22), a telescopic column (11) is penetrated inside the rotating cylinder (15), a mounting barrel (2) is installed above the telescopic column (11), a fixed column (3) is fixedly installed on the left side above the transmission plate (19), a second connecting bearing (7) is installed inside the fixed column (3), and a second connecting bearing (7) is installed inside the fixed column (3). A bearing (20) is provided, a second bevel gear (14) is installed on the right side of the fixing column (3), a first bevel gear (9) is installed below the second bevel gear (14), a transmission gear (8) is installed below the first bevel gear (9), a third connecting bearing (21) is installed inside the second bevel gear (14), a transmission rod (13) is installed on the right side of the second bevel gear (14), a bearing seat (23) is installed above the transmission rod (13), a connecting annular plate (16) is installed above the bearing seat (23), a second fixing cylinder (12) is installed above the connecting annular plate (16), a fifth connecting bearing (25) is installed inside the second fixing cylinder (12), and a connecting cylinder (10) is installed inside the fifth connecting bearing (25).

2. The oscillator for cell culture medium according to claim 1, characterized in that: The transmission plate (19) is movably connected to the first fixed cylinder (5) via the first connecting bearing (7), and the transmission plate (19) is movably connected to the rotating cylinder (15) via the fourth connecting bearing (22).

3. The oscillator for cell culture medium according to claim 1, characterized in that: The transmission gear (8) is fixedly connected to the rotating cylinder (15), and the transmission gear (8) is meshed with the internal gear (4). The diameter of the transmission gear (8) is smaller than the diameter of the internal gear (4).

4. The oscillator for cell culture medium according to claim 1, characterized in that: The second bevel gear (14) is movably connected to the fixed column (3) through the second connecting bearing (20), the first bevel gear (9) is fixedly connected to the rotating cylinder (15), and the second bevel gear (14) is meshedly connected to the first bevel gear (9).

5. The oscillator for cell culture medium according to claim 1, characterized in that: The transmission rod (13) is movably connected to the second bevel gear (14) via the third connecting bearing (21), and the transmission rod (13) is movably connected to the connecting annular plate (16) via the bearing seat (23).

6. The oscillator for cell culture medium according to claim 1, characterized in that: The second fixed cylinder (12) is fixedly connected to the mounting barrel (2), the second fixed cylinder (12) is movably connected to the connecting cylinder (10) via the fifth connecting bearing (25), and the connecting cylinder (10) is fixedly connected to the connecting annular plate (16).

7. The oscillator for cell culture medium according to claim 1, characterized in that: The rotating drum (15) is further provided with: A position-limiting movable groove (17) is provided on the inner wall of the rotating cylinder (15), and a position-limiting movable block (18) is movably installed inside the position-limiting movable groove (17).

8. The oscillator for cell culture medium according to claim 7, characterized in that: The limiting movable block (18) is fixedly connected to the telescopic column (11); the shape and size of the limiting movable block (18) match the shape and size of the interior of the limiting movable groove (17); and the telescopic column (11) is movably connected to the rotating cylinder (15) via the limiting movable groove (17) and the limiting movable block (18).

9. The oscillator for cell culture medium according to claim 1, characterized in that: The installation barrel (2) is further provided with: Rubber bands (24) are installed inside the installation barrel (2), and the rubber bands (24) are evenly distributed along the inside of the installation barrel (2).

10. The oscillator for cell culture medium according to claim 1, characterized in that: The housing (1) is further provided with: A control panel (26) is mounted on the front end of the housing (1), and the control panel (26) is electrically connected to the motor (6).

Images