Chlorella vulgaris and application thereof in carbon fixation and emission reduction and co-production of fertilizer

Abstract

The application discloses a chlorella and application of the chlorella in carbon fixation and emission reduction and co-production of biological fertilizer, and discloses a process for coupling microalgae carbon fixation and emission reduction of power plant flue gas and utilization of microalgae biological fertilizer, which comprises the following steps: 1) culturing the algal strain of the application in power plant flue gas; and 2) harvesting the algal strain cultured in step 1) and using the algal strain to prepare microalgae biological fertilizer. Through experiments, in view of the disadvantages of traditional treatment technologies for coal-fired power plant flue gas, the microalgae carbon fixation technology with the characteristics of environmental protection, economy, high efficiency and conformity to the natural cycle is adopted, and the microalgae biological fertilizer is coupled, so that the flue gas is treated and utilized, the entire ecological chain is prolonged, and the carbon fixation and emission reduction amount in the whole life cycle is effectively increased.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, and in particular to a type of Chlorella with high tolerance to power plant flue gas and its application in carbon sequestration, emission reduction and the production of bio-fertilizer. Background Technology

[0002] Coal-fired power generation is a significant source of carbon emissions, with flue gas containing large amounts of CO2 and trace pollutants, including SO2, NOx, and particulate matter.

[0003] Traditional CO2 treatment technologies for flue gas mainly include chemical and physical methods such as separation, absorption, recovery, and ammonia synthesis. However, traditional flue gas emission reduction technologies for coal-fired power plants generally suffer from problems such as complex processes and equipment, high energy consumption, and high treatment costs. There is still no mature decarbonization technology that can solve the CO2 emission problem of coal-fired power plants.

[0004] Microalgae are a type of photosynthetically autotrophic eukaryotic or prokaryotic microorganisms that convert CO2, water, and light energy into lipids, carbohydrates, and proteins through photosynthesis, enabling their growth. They are characterized by rapid growth and high photosynthetic carbon fixation efficiency; for example, their carbon fixation efficiency is 5-10 times that of typical terrestrial plants. Compared to traditional chemical and physical treatment technologies, microalgae flue gas carbon fixation and emission reduction technology is a novel technology combining biology and chemistry, offering advantages such as environmental friendliness, economy, high efficiency, and alignment with natural cycles. However, microalgae carbon fixation technology suffers from drawbacks such as a short lifespan and difficulties in subsequent microalgae treatment, which limits its large-scale application. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention isolates and screens a strain of *Chlorella vulgaris*, which, through pot experiments and cultivation in real power plant flue gas, demonstrates rapid growth, high stress resistance, and high fertilizer efficiency, adapting well to the characteristics of power plant flue gas emissions. The microalgae cultivation process achieves both flue gas carbon sequestration and emission reduction, as well as flue gas resource utilization. This couples power plant flue gas microalgae carbon sequestration and emission reduction with microalgae biofertilizer utilization, improving the economics of the technology while extending the entire ecological chain and effectively increasing the total carbon sequestration and emission reduction over the entire life cycle.

[0006] According to one aspect of the present invention, a strain of Parachlorella kessleri, Chlorella sp.HY1, is provided and deposited at the China Center for Type Culture Collection with accession number CCTCC NO: M 2025689.

[0007] According to another aspect of the present invention, the application of Chlorella vulgaris of the present invention in carbon sequestration and emission reduction in power plant flue gas and in the production of bio-fertilizer is provided.

[0008] According to an embodiment of the present invention, the Chlorella vulgaris is cultured in power plant flue gas.

[0009] According to another aspect of the present invention, a method is provided that couples microalgae carbon sequestration and emission reduction in power plant flue gas with the utilization of microalgae biofertilizer, comprising:

[0010] 1) Cultivating the algal strain of the present invention in power plant flue gas: and

[0011] 2) Harvest the algal strains cultivated in step 1) and use them to prepare microalgae bio-fertilizer.

[0012] According to an embodiment of the present invention, in step 1), the carbon fixation efficiency of the algal strain is above 0.5 g / L / d.

[0013] According to an embodiment of the present invention, step 2 includes compounding the harvested algae with other fertilizers to form microalgae bio-fertilizer, wherein the other fertilizers include inorganic fertilizers, organic fertilizers or bacterial bio-fertilizers.

[0014] According to an embodiment of the present invention, in step 2, the microalgae bio-fertilizer comprises an active microalgae fertilizer liquid product.

[0015] Compared to existing technologies, this invention provides a microalgae with better flue gas tolerance, higher carbon sequestration efficiency, and better fertilizer effect. Specifically, this invention achieves carbon sequestration and emission reduction in flue gas during microalgae cultivation. By coupling power plant flue gas microalgae carbon sequestration and emission reduction with microalgae bio-fertilizer utilization, it realizes the resource utilization of flue gas, improving the economics of the technology while extending the entire ecological chain, reducing subsequent microalgae waste emissions, and effectively increasing the total carbon sequestration and emission reduction throughout the entire life cycle. This process has advantages such as being environmentally friendly, economical, simple, efficient, and in line with the characteristics of natural cycles. Attached Figure Description

[0016] Figure 1 A photograph of the morphological characteristics of the *Parachlorella kessleri* strain *Chlorella sp. HY1* under an optical microscope, according to the present invention.

[0017] Figure 2 Phylogenetic tree of the 18S rDNA sequence of the *Parachlorella kessleri* strain *Chlorella sp. HY1* according to the present invention;

[0018] Figure 3 A process system diagram of coupling power plant flue gas microalgae carbon sequestration and emission reduction with microalgae bio-fertilizer utilization according to an embodiment of the present invention;

[0019] Figure 4 This is a schematic diagram of a microalgae cultivation system according to an embodiment of the present invention;

[0020] Figure 5 These are overall and root photos of lettuce under different treatments during the verification of the fertilizer effect of actual flue gas cultured Chlorella vulgaris liquid in flower pots, according to an embodiment of the present invention.

[0021] Figure 6 Figure 1 shows the experimental results of fresh weight of lettuce under different treatments according to the embodiments of the present invention.

[0022] Figure 7 The diagram shows the cell wall disruption effect of Chlorella according to an embodiment of the present invention.

[0023] Figure 8 The figure shows the experimental results of the growth and fresh weight of lettuce with different treatments according to another embodiment of the present invention. Detailed Implementation

[0024] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. The content shown is used to fully illustrate the content of the present invention, but is not intended to limit the present invention.

[0025] Unless otherwise specified, the experimental methods used in the embodiments are conventional methods, and the chemical and biological reagents used are conventional reagents in the art and are available for purchase.

[0026] Soil samples were collected from the field for soil microalgae enrichment. In a clean bench, 10g of the collected soil was weighed and transferred to a conical flask containing 90mL of sterile BG11 medium. The flask was sealed with gauze and sealing film, and then placed on a shaker and shaken (150rpm, 30min) to obtain a 10-fold dilution (10... -1 Soil suspension; 10 μL was aspirated using a pipette. -1 10 mL of the soil suspension was transferred to an Erlenmeyer flask containing 90 mL of sterile BG11 medium to obtain a 100-fold dilution (10). -2 The soil suspension was repeated to obtain 10 -3Soil suspension was collected. Erlenmeyer flasks were then placed in a light incubator (25±1℃, 3000 Lux continuous light) for enrichment culture. The flasks were shaken periodically, and enrichment culture was completed when the algal cell density reached 5000 cells / mL, as observed under a microscope. Algal purification was then performed. 5g of agar powder was weighed and poured into an Erlenmeyer flask containing 250mL of BG11 medium. After mixing, the flask was sealed with sealing film, and the flask was sterilized in an autoclave (121℃, 30min). After cooling to 50℃, the agar medium was poured into sterile petri dishes in a laminar flow hood. After cooling, 2% agar solid medium was obtained. A sterile inoculation loop was used to dab the enriched algal solution onto the agar solid medium. The petri dishes were then fully sealed with sealing film and placed in a light incubator (25±1℃, 3000 Lux continuous light) for continuous culture. After the colonies have grown on the solid culture medium, single colonies are picked out in a clean bench and sealed into a sterile centrifuge tube containing 5 mL of BG11 medium. The centrifuge tube is then placed in a light incubator for static culture for about 10 days. The algal solution is examined under an optical microscope. The algal solution free of contaminants and algae is transferred to a sterile conical flask containing 100 mL of BG11 medium for expansion culture to obtain a pure algal strain.

[0027] Morphological observation of the algal solution using an optical microscope revealed that the algal strain was an elliptical, 5-7 μm in size, non-flagellated single-celled green algae (see Appendix). Figure 1 The algal species was named Chlorella sp.HY1.

[0028] The algal species *Chlorella sp. HY1* was subjected to 18S rDNA sequencing by Sangon Biotech (Shanghai) Co., Ltd. The 18S rDNA sequence was then subjected to BLAST alignment analysis in the NCBI database. The alignment results showed that this algal species had 100% sequence coverage and 99.65% sequence similarity with *Parachlorella kessleri*. Homologous sequences with the highest alignment results in the NCBI database were selected, and homology alignment was performed using ClustalX software to obtain a multiple sequence matching permutation matrix. Then, a phylogenetic tree was constructed using Mega software with a neighbor-to-bootstraps algorithm and a bootstraps value of 1000 based on the 18S rDNA sequence (see Appendix). Figure 2 Analysis results indicate that the algal species is Parachlorella kessleri.

[0029] Therefore, this invention provides a Kjeldahl-type Chlorella, taxonomically named Chlorella sp. HY1, which was deposited on April 2, 2025, at the China Center for Type Culture Collection (CCTCC), located at No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province. The deposit date is April 2, 2025, and the accession number is CCTCC NO:M 2025689.

[0030] After obtaining and identifying the Chlorella strain of the Kjeldahl type, further experiments were conducted using this Chlorella strain.

[0031] Example 1: Tolerance experiment of algal strains in simulated flue gas

[0032] Figure 4 The figure shows a laboratory simulated flue gas culture system for Chlorella vulgaris strains according to an embodiment of the present invention. This system simulates the composition of power plant exhaust gas for microalgae culture. Generally, power plant exhaust gas contains CO2, SO2, and NOx, typically meeting the characteristics of ultra-low emission flue gas. However, in actual production, the composition of the flue gas can change due to factors such as production processes, especially SO2 and NOx. The concentration of these substances affects the growth and development of Chlorella vulgaris, thus affecting its carbon fixation effect. In this experiment, conditions other than flue gas ventilation, such as temperature, light, and CO2 concentration, were fixed. After single-factor screening with flue gas ventilation, the growth performance of different algal strains (including control strains) under different simulated flue gas concentrations was investigated. The experimental conditions are as follows:

[0033] Algal strain: Chlorella strain of the Kjeldahl class (in this invention); Control group algal strains: Chlorella ellipsoides and Scenedesmus;

[0034] Simulated flue gas: flue gas (SO2:NOx:N2 = 0.49%:0.98%:98.53%), high-purity CO2, and high-purity air;

[0035] Light and temperature: Light intensity approximately 100 μmol / m 2 / s, cultured in a water bath at a controlled temperature (28±0.5℃);

[0036] Microalgae cultivation system (see appendix) Figure 4The gas used in this experiment is a mixture of three gases: flue gas (an acidic mixture of NOx and SO2 at a certain concentration), high-purity air, and high-purity carbon dioxide from a cylinder. The actual concentrations of flue gas, air, and CO2 are controlled by adjusting the mass flow meter. An air filter is added to the inlet of a 400ml glass tube to remove bacteria from the gas. The gas flow rate is controlled by adjusting the mass flow meter and introduced into the 400ml glass tube through a glass tube with an aperture of approximately 2mm. The exhaust gas is collected and discharged into the atmosphere. The 400ml glass tube is placed in a thermostatic water bath made of highly transparent acrylic glass. The temperature of the water bath is controlled by a low-temperature thermostatic circulator. The light source used in the experiment is cold-light LEDs. The light intensity can be finely adjusted by adjusting the distance between the reactor and the light source. This culture system ensures that the light intensity, flue gas concentration, CO2 concentration, aeration rate, and temperature are all within a controllable range.

[0037] Experimental methods:

[0038] The activated algal strain was inoculated into a 400ml glass tube containing 300ml of BG11 liquid culture medium. The 400ml glass tube was placed in a water bath with circulation, and the water bath temperature was controlled at 28±0.5℃, the CO2 concentration at approximately 10%, and sterilization was performed using a microporous membrane filter. The simulated flue gas aeration rate was 0.2L / min, at approximately 100μmol / m³. 2 The cells were continuously illuminated by white LED tubes at a rate of / s, and the culture and screening cycle was set at 4 days. The cells were compared using relevant growth indicators.

[0039] First round: Chlorella vulgaris; Chlorella ellipsoides; Scenedesmus

[0040] (1) Initial inoculation amount: OD680 = 0.4;

[0041] (2) Flue gas: SO 2( 17.5mg / m 3 NOx (16.69 mg / m³) 3 ); An activation tolerance test was conducted under CO2: 10.0% conditions to examine the growth status of the algal strains;

[0042] (3) Algal strain culture: After 4 days of culture, it was found that:

[0043] (a) Under the experimental conditions, all three algal strains grew normally and no mortality was observed.

[0044] (b) Dry weight data showed that all three algal strains reached 2.3 g / L or higher, with the dry weight of Chlorella vulgaris strain reaching 2.67 g / L.

[0045] (c) The three algal strains are growing well and the differences are relatively small, so the next round of cultivation with increased concentration of flue gas will be carried out.

[0046] Second round: Chlorella vulgaris; Chlorella ellipsoides; Scenedesmus

[0047] (1) Initial inoculation amount: OD680 = 0.4;

[0048] (2) Flue gas: SO2: (35mg / m³) 3 NOx (33.39 mg / m³) 3 ); An activation tolerance test was conducted under CO2: 10.0% conditions to examine the growth status of the algal strains;

[0049] (3) Algal strain culture: After 4 days of culture, it was found that:

[0050] (a) The Scenedesmus could not adapt to the flue gas concentration and died on the second day. The pH of the algal solution was around 3.1, which was not suitable for the growth of the algal plants. The algal plants appeared white.

[0051] (b) Dry weight data: The dry weight of the surviving algal strains was all above 2.2 g / L;

[0052] (c) Using the two surviving algal strains, a third round of flue gas experiments was conducted to increase the flue gas concentration and proceed with the next algal strain experiment.

[0053] Third round: Chlorella strains of the Kjeldahl class; Chlorella ellipsoidale;

[0054] (1) Initial inoculation amount: OD680 = 0.4

[0055] (2) Flue gas: SO2 (78.9 mg / m³) 3 An activation tolerance test was conducted under the following conditions: NOx (75.88 mg / m3, approximately 60 ppm); CO2: approximately 11.5% to investigate the growth status of the algal strain.

[0056] (3) Algal strain culture: After 4 days of culture, it was found that:

[0057] (a) Dry weight data: The Chlorella strain of the Kjeldahl type grew normally and its dry weight reached 2.0 g / L;

[0058] (2) The elliptic Chlorella died on the first day, and the pH of the algal solution was about 2.8, and the algal solution turned milky white.

[0059] (3) Microscopic examination revealed that the Chlorella strains of the Kjeldahl class showed good activity and no aggregation.

[0060] The experimental results are shown in the table below:

[0061]

[0062] As shown above, after three rounds of algal strain tolerance tests with different flue gas concentrations, the flue gas concentration was gradually increased from a low concentration (SO₂). 2 From an initial concentration of 17.5 mg / m³ 3 Increased to approximately 78.9 mg / m³ 3 NO X From an initial concentration of approximately 16.7 mg / m³ 3 Increased to approximately 75.88 mg / m³ 3 (CO2 increased from 10% to about 11.5%). By comparing the performance indicators of the algal strains through a short 4-day culture period, it can be found that the Chlorella strain of the present invention has good tolerance in a wide range of flue gas concentrations, grows well, and its dry weight is basically above 2.0 g / L. Furthermore, microscopic examination revealed no aggregation.

[0063] Example 2: Cultivation of Chlorella vulgaris in simulated flue gas

[0064] In the laboratory, the composition of power plant flue gas was simulated using gas cylinders: SO2 (70 mg / m³). 3 NO x (67mg / m 3 The activated Chlorella vulgaris culture was inoculated into a 400ml glass tube containing 300mL of BG11 medium, with an inoculation concentration of 0.1g / L. The 400mL glass tube was placed in a water bath with a circulating temperature controlled at 28±0.5℃, simulating a flue gas aeration rate of 0.2L / min, and approximately 100μmol / m³. 2 After 8 days of continuous illumination under a white LED tube, the microalgae biomass was measured at the end of the experiment. The maximum dry weight was 4.1 g / L and the average carbon fixation rate was 0.943 g / L / d.

[0065] Example 3: Cultivation of Chlorella vulgaris using power plant flue gas

[0066] Actual emissions from a coal-fired power plant (ultra-low emission standard: approximately 10.0% CO2, 35.2 mg / m³) were measured. 3 SO2, 49.7 mg / m³ 3 The carbon fixation and growth performance of microalgae were evaluated using NOx-free methods. Activated Chlorella vulgaris strains were inoculated into a 10L column reactor containing 7 LB G11 medium, which was placed inside a container on-site. The inoculation concentration was 0.1 g / L. The indoor temperature was controlled at 25℃ by air conditioning, the actual flue gas aeration rate was 5 L / min, and the algae were continuously cultured under approximately 15000 Lux white LED lights for 8 days. After the experiment, the microalgal biomass was measured, reaching a maximum dry weight of 3.5 g / L and an average carbon fixation rate of 0.805 g / L / d.

[0067] Example 4: Cultivation of Chlorella vulgaris using power plant flue gas

[0068] At the coal-fired power plant site, actual emissions of flue gas (ultra-low emission standard: approximately 10.0% CO2, 35.2 mg / m³) were measured. 3 SO2, 49.7 mg / m³ 3 The carbon fixation and growth performance of microalgae were evaluated using NOx-free methods. Activated Chlorella vulgaris strains were inoculated into a 10L column reactor containing 7 LB G11 medium, which was placed inside a container on-site. The inoculation concentration was 0.1 g / L. The indoor temperature varied from 15-35℃, the actual flue gas aeration rate was 6 L / min, and the daytime sunlight intensity varied from approximately 1500-25000 Lux. The experiment was conducted for 8 days. After the experiment, the microalgal biomass was measured, reaching a maximum dry weight of 2.4 g / L and an average carbon fixation rate of 0.552 g / L / d.

[0069] Example 5: Experiment on Chlorella liquid bio-fertilizer (Kjeldahl class)

[0070] Figure 3 This paper illustrates the process flow of coupling microalgae carbon sequestration and emission reduction with microalgae biofertilizer utilization in power plant flue gas according to an embodiment of the present invention. In this embodiment, a potted plant fertilization efficiency verification experiment was conducted using actual flue gas culture of *Chlorella vulgaris* from Example 4. Lettuce was used as the model plant, and four treatments were set up: treatment 1 (control, CK), treatment 2 (C1, 15 mL algae solution), treatment 3 (C2, 30 mL algae solution), and treatment 4 (C3, 50 mL algae solution), with three replicates for each treatment. Each pot contained 2 L of nutrient soil (organic fertilizer and coconut coir, 1:1 (v / v)). Twelve lettuce plants of the same size and growth status were selected, and one seedling was planted in each pot. Fertilization was performed on the day of lettuce transplanting. Each pot was first irrigated with 100 mL of garden nutrient solution (GNS), and then 15, 30, and 50 mL of algae solution were added to treatments 2, 3, and 4, respectively. On the fourth day, 50 mL of water was added to each treatment group. On days 7 and 11, each treatment group was irrigated with 100 mL of GNS. After 20 days of cultivation, the top layer of lettuce leaves was harvested. Figure 5 The images show the overall shape and root portion of lettuce treated at harvest, along with fresh weight measurements. The fresh weights for each treatment are shown below. Figure 6 As shown in the figure, compared with the control group without algal solution, the fresh weight of the treatment groups treated with 15 mL, 30 mL, and 50 mL of algal solution increased by 18.0%, 23.0%, and 32.8%, respectively. The results indicate that the Chlorella vulgaris of the present invention has significant fertilizer efficacy.

[0071] Example 6: Experiment on biofertilizer made from Chlorella lysate of Kjeldahl algae

[0072] The Chlorella algae liquid harvested in Example 4 was subjected to cell wall disruption treatment using a high-pressure homogenizer. The pressure was adjusted to 1000-1400 bar, and the mixture was circulated 3-4 times. The cell wall disruption temperature was controlled at 4°C using a circulating cooling water system. The cell wall disruption rate of the Chlorella after disruption was over 90%. For details on the cell wall disruption effect, please refer to the appendix. Figure 7 .

[0073] The microalgae lysate obtained above was used as the experimental fertilizer. Lettuce was used as the model plant, and four treatments were set up: Treatment 1 (control, CK), Treatment 2 (C1, 15 mL microalgae lysate), Treatment 3 (C2, 30 mL microalgae lysate), and Treatment 4 (C3, microalgae lysate, 50 mL algae solution), with three replicates for each treatment. Each pot contained 2 L of nutrient soil (organic fertilizer and coconut coir, 1:1 (v / v)). Twelve lettuce plants of similar size and growth status were selected, and one seedling was planted in each pot. Fertilization was performed on the day of transplanting. Each pot was first irrigated with 100 mL of garden nutrient solution (GNS), and then 15, 30, and 50 mL of microalgae lysate were added to Treatments 2, 3, and 4, respectively. On the 4th day, 50 mL of water was added to each treatment group. On the 7th and 11th days, each treatment group was watered with 100 mL of GNS. After 20 days of cultivation, the top layer of lettuce leaves was harvested and their fresh weight was measured. The fresh weight of each treatment is as follows: Figure 8 As shown in the figure, compared with the control group without algal solution, the fresh weight of the treatment groups treated with 15 mL, 30 mL, and 50 mL of algal solution increased by 13.8%, 26.2%, and 43.1%, respectively. The results indicate that the Chlorella vulgaris of the present invention has significant fertilizer efficacy.

[0074] Using microalgae for flue gas carbon sequestration and emission reduction has many advantages: 1) Microalgae grow rapidly and have strong vitality. Their CO2 fixation capacity is 10-50 times that of ordinary forests, and their biomass yield is 4.5 times that of soybeans; 2) Microalgae can be cultivated and sequestered on non-arable land or in seawater, so they will not cause changes in land properties, thereby minimizing the related environmental impacts; 3) Microalgae can be grown and cultivated year-round, making them suitable for flue gas emissions from coal-fired power plants that operate year-round.

[0075] This invention isolated a strain of *Chlorella vulgaris*, which exhibits good tolerance to flue gas concentrations over a wide range and grows well and rapidly, making it highly suitable for direct application in carbon sequestration and emission reduction from power plant flue gas. Furthermore, this invention also revealed that this microalgae possesses excellent fertilizing properties, making it suitable as a microalgae bio-fertilizer with significant economic value. Based on these findings, this invention innovatively couples microalgae carbon sequestration and emission reduction from power plant flue gas with the utilization of microalgae bio-fertilizer, greatly extending the ecological chain of this technology, effectively increasing the total carbon sequestration and emission reduction over the entire life cycle, while simultaneously reducing subsequent waste emissions and treatment, lowering costs, and demonstrating excellent economic feasibility.

[0076] The above description of the embodiments is intended to enable those skilled in the art to understand and apply the present invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the embodiments described herein, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. Parachlorella kessleri strain HY1, characterized in that, It is deposited at the China Center for Type Culture Collection with accession number CCTCC NO: M 2025689.

2. The application of the Chlorella vulgaris species described in claim 1 in carbon sequestration, emission reduction, and co-production of microalgae bio-fertilizer in power plant flue gas, characterized in that... The Chlorella species of the Kjeldahl class are cultured in power plant flue gas, and the resulting algal strains are used to prepare microalgae bio-fertilizer, which is used to promote the growth of lettuce.

3. A method for coupling microalgae carbon sequestration and emission reduction in power plant flue gas with the utilization of microalgae biofertilizer, characterized in that, include: Step 1) Cultivate the algal strain of claim 1 in the flue gas of a power plant; as well as Step 2) Harvest the algal strains cultivated in Step 1) and use them to prepare microalgae bio-fertilizer, which is used to promote the growth of lettuce.

4. The method for coupling microalgae carbon sequestration and emission reduction in power plant flue gas with microalgae biofertilizer utilization according to claim 3, characterized in that, In step 1), the carbon fixation efficiency of the algal strain is above 0.5 g / L / d.

5. The method for coupling microalgae carbon sequestration and emission reduction in power plant flue gas with microalgae biofertilizer utilization according to claim 3, characterized in that, Step 2) involves compounding the harvested algae with other fertilizers to form microalgae bio-fertilizer, wherein the other fertilizers include inorganic fertilizers, organic fertilizers, or bacterial bio-fertilizers.

6. The method for coupling microalgae carbon sequestration and emission reduction in power plant flue gas with microalgae biofertilizer utilization according to claim 3, characterized in that, In step 2), the microalgae bio-fertilizer includes an active microalgae bio-fertilizer liquid product.

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