Saccharomyces cerevisiae genetically engineered bacterium for producing gamma-aminobutyric acid as well as construction method and application thereof

By modifying the Saccharomyces cerevisiae strain, overexpressing the mutant glutamate decarboxylase GAD/D360M and integrating the PdxS expression cassette, an engineered Saccharomyces cerevisiae strain that efficiently produces GABA was constructed. This solved the high cost of whole-cell catalytic production of GABA in Saccharomyces cerevisiae, achieving efficient and low-cost GABA production and resource utilization of industrial waste.

CN120591249APending Publication Date: 2025-09-05SHANDONG UNIV
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Patent Information

Application Number
CN202510770779.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-10
Publication Date
2025-09-05

AI Technical Summary

Technical Problem

The existing technology for producing γ-aminobutyric acid (GABA) by whole-cell catalysis of Saccharomyces cerevisiae is costly, mainly due to the requirement of expensive cofactor 5'-pyridoxal phosphate (PLP), and there are no reports on the rational design of the global metabolic pathway of Saccharomyces cerevisiae combined with whole-cell catalysis.

Method used

By modifying the Saccharomyces cerevisiae strain, overexpressing the mutant glutamate decarboxylase GAD/D360M, knocking out the GABA transaminase uga1 gene, and integrating the 5'-pyridoxal phosphate synthase PdxS expression cassette, a genetically engineered Saccharomyces cerevisiae strain that efficiently produces GABA was constructed, using glutamate waste liquid for whole-cell catalysis.

Benefits of technology

The efficient production of GABA was achieved without adding PLP, which reduced production costs, increased output, and realized the resource utilization of industrial waste.

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Abstract

The invention belongs to the technical field of microorganisms and metabolic engineering, and particularly relates to a saccharomyces cerevisiae genetically engineered bacterium for producing gamma-aminobutyric acid (GABA) as well as a construction method and application of the saccharomyces cerevisiae genetically engineered bacterium. The saccharomyces cerevisiae gene engineering strain capable of efficiently catalyzing and synthesizing GABA is obtained through rational metabolism modification means such as modifying a GABA synthesis key enzyme glutamate decarboxylase and overexpressing the GABA synthesis key enzyme glutamate decarboxylase in saccharomyces cerevisiae, blocking a GABA degradation pathway and increasing GABA synthesis cofactor supply. The strain is high in safety, a fermentation culture medium is composed of industrial by-products, the strain grows rapidly and is low in cost, and high conversion rates can be obtained through whole-cell catalysis of L-glutamic acid and glutamic acid waste liquid. The method provides possibility for large-scale industrial production of GABA by using saccharomyces cerevisiae as excellent chassis cells and resource utilization of glutamic acid waste liquid, and has good practical application value.
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