Formula nutrition preparation for preventing and intervening Alzheimer's disease and preparation method thereof

Abstract

The invention relates to the technical field of formula nutrition preparations, in particular to a formula nutrition preparation for preventing and intervening Alzheimer's disease and a preparation method of the formula nutrition preparation for preventing and intervening Alzheimer's disease. Comprising the following raw materials: 3-6 parts of a phosphatidylserine compound, 10-20 parts of refined fish oil, 1-3 parts of a grape seed extract, 50-150 parts of medium chain triglyceride, 1-3 parts of epigallocatechin gallate, 5-10 parts of blueberry powder, 5-10 parts of sea-buckthorn powder, 5-10 parts of blackcurrant powder, 2-5 parts of phospholipid, 10-30 parts of xylooligosaccharide and 0.5-1 part of B vitamins. The formula nutrition preparation for preventing and intervening the Alzheimer's disease, prepared by the invention, can improve the intelligence improving effect by intervening a plurality of pathological pathways and exerting a multi-target synergistic effect, and has the characteristic of being easy to absorb.

Description

Technical Field

[0001] The present invention relates to the technical field of nutritional formula preparations, and in particular to a nutritional formula preparation for preventing and intervening in Alzheimer's disease and a preparation method thereof. Background Art

[0002] Alzheimer's disease is a complex brain disease syndrome involving multiple pathological pathways. Its main pathological features are Aβ plaques, abnormal phosphorylation of Tau protein, neuroinflammation, neurotransmitter changes, oxidative stress, cerebrovascular abnormalities, intestinal flora imbalance, etc. Existing drugs (such as cholinesterase inhibitors) have problems such as large side effects and limited efficacy, and safe and effective adjuvant therapies are urgently needed. Currently, there is no specific drug or surgical technique that can effectively treat Alzheimer's disease, but early and timely supplementation of nutrients can effectively prevent it. Even if symptoms of Alzheimer's disease exist, timely supplementation of brain nutrients can also avoid the deterioration of related symptoms to a certain extent. Existing health products used to prevent or intervene in Alzheimer's disease still have the following defects: (1) The effect of a single ingredient (such as ginkgo leaf extract and curcumin) is limited and lacks multi-target synergy; (2) low bioavailability (such as curcumin's poor water solubility) and insufficient stability.

[0003] Patent CN105311072A discloses a health product for improving memory and its preparation method. The health product is mainly made from the following raw materials by weight: 100-600 parts of phosphatidylserine, 1000-3000 parts of fish oil, and 100-600 parts of Acer truncatum seed oil. Acer truncatum seed oil is rich in neuraminic acid, which repairs and dredges damaged brain neural pathways (nerve fibers), promotes nerve cell regeneration, and promotes the body's absorption and utilization of the nutrients in fish oil and phosphatidylserine. Phosphatidylserine can improve nerve cell function, regulate the transmission of nerve impulses, and enhance brain memory. After absorption, it can enter the brain through the blood-brain barrier, soothing vascular smooth muscle cells and increasing blood supply to the brain. At the same time, phosphatidylserine and fish oil work synergistically, promoting mutual absorption and protecting nerve cells. However, if phosphatidylserine is consumed directly, its absorption efficiency is low, which can affect its mental benefits.

[0004] Patent CN118415343A discloses a phosphatidylserine multi-dimensional nutrient composition with the potential to improve memory. Per 100g of the multi-dimensional nutrient composition, the composition contains 0.5g-5g of phosphatidylserine, 0.5g-3g of γ-aminobutyric acid, 1-5g of kudzu root extract, 1-3g of taurine, and the remainder is virgin coconut oil microcapsule powder and enzymatically hydrolyzed oatmeal. This inventive complex boasts high purity, rich nutrition, a scientifically formulated formula, and is convenient for consumption and portability. It is said to enhance memory, improve blood circulation and sleep quality, regulate neuronal activity, and protect nerves. However, its formula is relatively simple in composition, failing to achieve multi-target synergistic effects.

[0005] Therefore, there is an urgent need in the market for a formula nutritional preparation for preventing and intervening in Alzheimer's disease that can exert multi-target synergistic effects and has excellent intelligence-enhancing effects. Summary of the Invention

[0006] In response to the problems existing in the prior art, the purpose of the present invention is to obtain a formula nutritional preparation for preventing and intervening in Alzheimer's disease that enhances the intelligence-enhancing effect through a multi-nutrient synergistic nutrient cocktail therapy mechanism and is easily absorbed.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] On the one hand, the present invention provides a formula nutritional preparation for preventing and intervening in Alzheimer's disease, which includes the following raw materials, in parts by weight: 3-6 parts of phosphatidylserine complex, 10-20 parts of refined fish oil, 1-3 parts of grape seed extract, 50-150 parts of medium-chain triglycerides, 1-3 parts of epigallocatechin gallate, 5-10 parts of blueberry powder, 5-10 parts of sea buckthorn powder, 5-10 parts of blackcurrant powder, 2-5 parts of phospholipids, 10-30 parts of xylo-oligosaccharides, and 0.5-1 part of B vitamins.

[0009] This application uses scientific compatibility and synergy between its ingredients to provide a one-stop supplement for the brain's multiple nutritional elements. Phospholipids and phosphatidylserine work synergistically with DHA from fish oil to protect and repair nerve cells, promote synaptic remodeling, improve cognitive function, and significantly alleviate cognitive decline. Phosphatidylserine and EPA from fish oil work synergistically to regulate mood and alleviate anxiety or depression. Phospholipids and EPA from fish oil work synergistically to optimize vascular elasticity, slow aging of brain microvessels, and keep blood vessels unobstructed. Grape seed extract prevents oxidation of various substances in the formula.

[0010] In addition, the various substances in the formula nutritional preparations can enhance the intellectual effect of the formula nutritional preparations by jointly intervening in multiple pathological pathways through multiple targets. Among them, phosphatidylserine can improve the function of nerve cells and improve sleep mechanism; grape seed extract contains more than 95% oligomeric proanthocyanidins, which can improve the body's antioxidant capacity, regulate cytokine levels, improve cell inflammation, lower blood pressure, and improve arteriosclerosis; epigallocatechin gallate and different polyphenol compounds contained in blueberry powder, sea buckthorn powder, and blackcurrant powder can not only bidirectionally regulate intestinal flora, maintain intestinal health, improve immunity, inhibit neuroinflammation, protect neurons, and reduce the risk of Alzheimer's disease, but also enhance Digestive system function, promote the matrix's full absorption of nutrients, and then ensure the health of the brain through the "gut-brain axis"; medium-chain triglycerides can quickly supply energy, increase the brain's energy source, and thus enhance brain vitality and improve cognitive function; the active ingredients in refined fish oil can reduce neuroinflammation; phospholipids contain antioxidant groups that can protect nerve cells from oxidative damage; oligoxylose can regulate intestinal flora, enhance immunity, control blood sugar and lower blood lipids, regulate metabolism, promote mineral absorption, and reduce toxins in the intestine from entering the blood to protect the liver; B vitamins may delay the progression of Alzheimer's disease by regulating homocysteine ​​metabolism, anti-oxidation, and improving mitochondrial function.

[0011] In some embodiments, the method for preparing the phosphatidylserine complex comprises the following steps:

[0012] A1. Add phosphatidylserine to deionized water, add an emulsifier, stir, and homogenize to obtain solution 1;

[0013] A2. Add lecithin and resveratrol-galactoside to deionized water, stir at high speed at 40-50° C. for 20-30 min to obtain solution 2. Add solution 1 obtained in step A1 to solution 2, stir to obtain a uniform emulsion, and spray dry to obtain a phosphatidylserine complex.

[0014] Preferably, the lecithin is egg yolk lecithin.

[0015] This application uses resveratrol-galactoside modified lecithin to combine the two through conjugation, hydrogen bonding and electrostatic forces, which not only improves the dispersion performance of lecithin in water and thus reduces the use of solvents, but is also more conducive to food safety and prolongs the peak time of resveratrol to improve its bioavailability. This application further compounds resveratrol-galactoside modified lecithin with phosphatidylserine to obtain a phosphatidylserine complex, which can achieve targeted sustained release of phosphatidylserine in the intestine and improve the bioavailability of phosphatidylserine. Resveratrol can also exert significant synergistic effects with phosphatidylserine in terms of anti-oxidation, anti-inflammatory, neuroprotection, immunomodulation, and improvement of the cardiovascular system, which can further enhance the intellectual effect of the formulated nutritional preparation. In addition, the benzene rings on resveratrol and galactoside, epigallocatechin gallate, and different polyphenol compounds contained in blueberry powder, sea buckthorn powder, and blackcurrant powder make it easier for phosphatidylserine to be surrounded by polyphenol compounds after release through the π-π conjugation effect. The antioxidant effect of polyphenol compounds can reduce the oxidative damage of PS during the release process, thereby prolonging its effective action time in the body, especially in the brain, which is very sensitive to oxidative stress, thereby significantly enhancing the intellectual effect of the formula nutritional preparation.

[0016] In some embodiments, the mass ratio of the lecithin to resveratrol-galactoside is 1:(0.1-0.4).

[0017] In some embodiments, the mass ratio of lecithin to phosphatidylserine is 1:(0.2-0.4).

[0018] The present application can further enhance the intelligence-enhancing effect of the nutritional preparation for preventing and intervening in Alzheimer's disease by limiting the mass ratios of lecithin and resveratrol-galactoside and lecithin and phosphatidylserine. This may be because at this ratio, the phosphatidylserine complex has a higher loading rate and better sustained-release effect.

[0019] In some embodiments, the method for preparing resveratrol-galactoside comprises the following steps:

[0020] B1. Add lactose to a phosphate buffer solution to obtain a lactose solution. Add resveratrol, β-galactosidase, and the lactose solution to acetone. React at 40-50° C. for 40-60 minutes, then heat to 100° C. and boil for 4-6 minutes to obtain a reaction solution.

[0021] B2. Centrifuge the reaction solution obtained in step B1 for 15-25 minutes, draw the supernatant, adsorb it with a macroporous adsorption resin, elute it with an ethanol gradient, and freeze-dry it to obtain resveratrol-galactoside.

[0022] Preferably, the mass ratio of lactose to β-galactosidase is 1:(0.010-0.016).

[0023] The resveratrol-galactoside prepared in the present application can be catalyzed by β-galactosidase in the brain to break the glycosidic bond, thereby releasing resveratrol to act on brain cells, thereby improving the bioavailability and brain permeability of resveratrol in the brain and enhancing the intellectual effect of the formulated nutritional preparation.

[0024] In some embodiments, the mass ratio of lactose to resveratrol is 1:(0.55-0.65).

[0025] The present application can further enhance the intelligence-enhancing effect of the nutritional preparation for preventing and intervening in Alzheimer's disease by limiting the mass ratio of lactose to resveratrol. This may be because under this ratio, resveratrol can be released in time and act on brain cells after the glycosidic bond is broken.

[0026] In some embodiments, the macroporous adsorption resin is of model XAD-16.

[0027] In some embodiments, the phospholipid is a composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylserine, and the mass ratio of the four is 1:(0.15-0.18):(0.05-0.1):(0.2-0.3).

[0028] In some embodiments, the B vitamins are a combination of B6, B9, and B12, and the mass ratio of the three is 1: (0.25-0.31): (0.001-0.002).

[0029] In some embodiments, the phospholipid is soy lecithin.

[0030] On the other hand, the present invention provides a method for preparing a formula nutritional preparation for preventing and intervening in Alzheimer's disease, comprising the following steps: uniformly mixing phosphatidylserine complex, refined fish oil, grape seed extract, medium-chain triglycerides, epigallocatechin gallate, blueberry powder, sea buckthorn powder, blackcurrant powder, phospholipids, oligoxylose, and B vitamins to obtain the formula nutritional preparation.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) The present invention can supplement the various nutrients needed by the brain in one stop through the scientific compatibility and mutual synergy of the various components. Among them, phospholipids and DHA play a synergistic role in protecting and repairing nerve cells, promoting the remodeling of synapses and improving cognitive function; phosphatidylserine and fish oil can promote each other's absorption and utilization. The combined supplementation of PS + DHA can significantly improve cognitive decline, and PS + EPA can jointly regulate mood and relieve anxiety / depression tendencies; phospholipids and EPA in fish oil keep blood vessels unobstructed; grape seed extract prevents the oxidation of various substances.

[0033] (2) The present invention intervenes in multiple pathological pathways, making the health care products used to prevent or improve Alzheimer's disease have better mental health effects. Grape seed extract improves the body's antioxidant capacity; EGCG and different polyphenol compounds contained in blueberry powder, sea buckthorn powder, and blackcurrant powder regulate intestinal flora in a two-way manner, thereby affecting brain health through the "gut-brain axis"; medium-chain triglycerides can quickly supply energy and solve the problem of low glucose metabolism in patients; unsaturated fatty acids DHA / EPA in refined fish oil can reduce neuroinflammation; antioxidant groups in phospholipids can protect nerve cells from oxidative damage; oligoxylose can regulate intestinal flora, enhance immunity, control blood sugar and lower blood lipids, regulate metabolism, promote mineral absorption, reduce the entry of toxins in the intestine into the blood, and play a liver-protecting role.

[0034] (3) The present invention improves the dispersion performance of lecithin in water by using resveratrol-galactoside modified lecithin and then compounds it with phosphatidylserine to obtain a phosphatidylserine complex, which can target the release of phosphatidylserine in the intestine, thereby not only improving the bioavailability of phosphatidylserine but also reducing intestinal irritation. Resveratrol can also have significant synergistic effects with phosphatidylserine in terms of anti-oxidation, anti-inflammatory, and neuroprotection, which can further enhance the intellectual effect of health products. DETAILED DESCRIPTION

[0035] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following examples are illustrative of the present invention and are intended only to illustrate the present invention and are not intended to limit the present invention. Other combinations and various modifications within the scope of the present invention may be made without departing from the spirit or scope of the present invention.

[0036] In the following examples and comparative examples, except for the phosphatidylserine complex, the other compounds and related reagents used can be purchased from the market, among which the refined fish oil is Australian Nu-Mega deep-sea fish oil, and the grape seed extract is French Indena grape seed extract.

[0037] Preparation Example 1

[0038] The preparation method of resveratrol-galactoside-1 comprises the following steps:

[0039] B1. Add 1 g of lactose to 10 g of IH012-PBS phosphate buffer solution to obtain a lactose solution. Add 0.6 g of resveratrol, 0.013 g of β-galactosidase, and the lactose solution to 50 g of acetone. React at 45° C. for 50 min, then heat to 100° C. and boil for 5 min to obtain a reaction solution.

[0040] B2. Centrifuge the reaction solution obtained in step B1 for 20 minutes, draw the supernatant, adsorb it with XAD-16 macroporous adsorption resin, elute it with ethanol gradient, and freeze-dry it to obtain resveratrol-galactoside-1.

[0041] Preparation Example 2

[0042] The preparation method of resveratrol-galactoside-2 is the same as that of Preparation Example 1, except that the added amount of resveratrol is 0.45 g.

[0043] Preparation Example 3

[0044] The preparation method of phosphatidylserine complex-1 comprises the following steps:

[0045] A1. Add 3 g of phosphatidylserine to 50 g of deionized water, add 0.05 g of Tween 80 emulsifier, stir, and homogenize to obtain Solution 1;

[0046] A2. Add 10 g of lecithin and 2.5 g of resveratrol-galactoside-1 to 100 g of deionized water, and stir at 45° C. for 25 min to obtain solution 2. Add solution 1 obtained in step A1 to solution 2, stir to obtain a uniform emulsion, and spray dry to obtain phosphatidylserine complex-1.

[0047] Preparation Example 4

[0048] The preparation method of phosphatidylserine complex-2 is the same as that of Preparation Example 3, except that the added amount of resveratrol-galactoside-1 is 5 g.

[0049] Preparation Example 5

[0050] The preparation method of phosphatidylserine complex-3 is the same as that of Preparation Example 3, except that the amount of phosphatidylserine added is 5 g.

[0051] Preparation Example 6

[0052] The preparation method of phosphatidylserine complex-4 is the same as that of Preparation Example 3, except that an equal amount of resveratrol-galactoside-1 is replaced by resveratrol-galactoside-2.

[0053] Example 1

[0054] A nutritional preparation for preventing and intervening in Alzheimer's disease comprises the following ingredients, measured in parts by weight: 4 parts of phosphatidylserine complex-1, 15 parts of refined fish oil, 2 parts of grape seed extract, 100 parts of medium-chain triglycerides, 2 parts of epigallocatechin gallate, 7 parts of blueberry powder, 7 parts of sea buckthorn powder, 8 parts of blackcurrant powder, 3 parts of phospholipids, 15 parts of xylo-oligosaccharides, and 0.7 parts of B vitamins.

[0055] The phospholipid is a composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, and the mass ratio of the four is 1:0.16:0.07:0.25.

[0056] B vitamins are a combination of B6, B9, and B12, with the mass ratio of the three being 1:0.28:0.0015.

[0057] The preparation method of the formula nutritional preparation for preventing and intervening in Alzheimer's disease in this embodiment comprises the following steps: phosphatidylserine complex-1, refined fish oil, grape seed extract, medium-chain triglycerides, epigallocatechin gallate, blueberry powder, sea buckthorn powder, blackcurrant powder, phospholipids, oligoxylose, and B vitamins are uniformly mixed at room temperature to obtain the preparation.

[0058] Example 2

[0059] A nutritional preparation for preventing and intervening in Alzheimer's disease comprises the following ingredients, measured in parts by weight: 3 parts of phosphatidylserine complex-1, 10 parts of refined fish oil, 1 part of grape seed extract, 50 parts of medium-chain triglycerides, 1 part of epigallocatechin gallate, 5 parts of blueberry powder, 5 parts of sea buckthorn powder, 5 parts of blackcurrant powder, 2 parts of phospholipids, 10 parts of xylo-oligosaccharides, and 0.5 parts of B vitamins.

[0060] The phospholipid is a composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, and the mass ratio of the four is 1:0.15:0.05:0.2.

[0061] B vitamins are a combination of B6, B9, and B12, with the mass ratio of the three being 1:0.25:0.001.

[0062] The preparation method of the formula nutritional preparation for preventing and intervening in Alzheimer's disease in this embodiment comprises the following steps: phosphatidylserine complex-1, refined fish oil, grape seed extract, medium-chain triglycerides, epigallocatechin gallate, blueberry powder, sea buckthorn powder, blackcurrant powder, phospholipids, oligoxylose, and B vitamins are uniformly mixed at room temperature to obtain the preparation.

[0063] Example 3

[0064] A nutritional preparation for preventing and intervening in Alzheimer's disease comprises the following ingredients, measured in parts by weight: 6 parts of phosphatidylserine complex-1, 20 parts of refined fish oil, 3 parts of grape seed extract, 150 parts of medium-chain triglycerides, 3 parts of epigallocatechin gallate, 10 parts of blueberry powder, 10 parts of sea buckthorn powder, 10 parts of blackcurrant powder, 5 parts of phospholipids, 30 parts of xylo-oligosaccharides, and 1 part of B vitamins.

[0065] The phospholipid is a composition of phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylserine, and the mass ratio of the four is 1:0.18:0.1:0.3.

[0066] B vitamins are a combination of B6, B9, and B12, with the mass ratio of the three being 1:0.31:0.002.

[0067] The preparation method of the formula nutritional preparation for preventing and intervening in Alzheimer's disease in this embodiment comprises the following steps: phosphatidylserine complex-1, refined fish oil, grape seed extract, medium-chain triglycerides, epigallocatechin gallate, blueberry powder, sea buckthorn powder, blackcurrant powder, phospholipids, oligoxylose, and B vitamins are uniformly mixed at room temperature to obtain the preparation.

[0068] Example 4

[0069] A nutritional formulation for preventing and intervening in Alzheimer's disease and a preparation method thereof. The specific implementation method is the same as that of Example 1, except that an equal amount of phosphatidylserine complex-1 is replaced by phosphatidylserine complex-2.

[0070] Example 5

[0071] A nutritional formulation for preventing and intervening in Alzheimer's disease and a preparation method thereof. The specific implementation method is the same as that of Example 1, except that an equal amount of phosphatidylserine complex-1 is replaced by phosphatidylserine complex-3.

[0072] Example 6

[0073] A nutritional formulation for preventing and intervening in Alzheimer's disease and a preparation method thereof. The specific implementation method is the same as that of Example 1, except that an equal amount of phosphatidylserine complex-1 is replaced by phosphatidylserine complex-4.

[0074] Comparative Example 1

[0075] A nutritional formula for preventing and intervening in Alzheimer's disease and a preparation method thereof. The specific implementation method is the same as that of Example 1, except that an equal amount of phosphatidylserine complex-1 is replaced by phosphatidylserine.

[0076] Performance Testing

[0077] The nutritional preparations for preventing and intervening in Alzheimer's disease obtained in the above-mentioned examples and comparative examples were evaluated for their intelligence-enhancing effects and safety:

[0078] Nootropic effects:

[0079] 1. Mouse behavioral experiments

[0080] 1.1 Experimental Animals

[0081] Thirty-five SPF female ICR mice weighing 30 ± 5 g were housed in a barrier-free animal room with free access to food and water throughout the experiment. The room temperature was 20°C-24°C and the relative humidity was 40%-70%. After seven days of acclimatization with a complete pelleted mouse diet, the mice were randomly divided into five groups of six mice each based on body weight and subjected to the shuttle box test. Six mice in each group served as negative and positive controls, as well as experimental mice receiving different doses of the test substance.

[0082] 1.2 Dose selection

[0083] The dosage for mice was calculated based on the recommended dosage for humans: 0.30 g of phosphatidylserine, 7.5 g, 15 g, and 30 g of the formula nutritional preparation for the prevention and intervention of Alzheimer's disease obtained in Example 1 were added to 100 ml of sterile water at 40°C, and the gavage volume was 10 ml / kg, resulting in a low dose of 0.75 g / kg.bw, a medium dose of 1.5 g / kg.bw, and a high dose of 3.0 g / kg.bw.

[0084] 1.3 Experimental Group

[0085] Negative control group: gavage with 10 ml / kg.bw sterile water;

[0086] Positive control group: oral administration of 0.03 g / kg.bw phosphatidylserine;

[0087] Low-dose group: Oral administration of 0.75g / kg.bw of a nutritional preparation for the prevention and intervention of Alzheimer's disease;

[0088] Medium-dose group: Oral administration of 1.5g / kg.bw of a nutritional preparation for the prevention and intervention of Alzheimer's disease;

[0089] High-dose group: Oral administration of 3.0g / kg.bw of a nutritional preparation for the prevention and intervention of Alzheimer's disease.

[0090] 1.4 Experimental methods

[0091] The mice were gavaged once daily for 4 consecutive weeks, and training was performed in the early stage. After the last gavage, the mice were subjected to the shuttle box test. During the experiment, the mice in each group had normal food intake and free access to water.

[0092] 1.5 Mouse shuttle box test

[0093] (1) Training period: Each mouse moves freely in the test box for 1 minute to eliminate the exploratory reflex. The animal is placed in the electric shock area of ​​the shuttle test box. First, the conditioned stimulus (light) and / or buzzer (pure tone stimulus) are given for 15 seconds (adjustable duration), and then an electric stimulus of 0.2 mA (current intensity is 0.2-0.8 mA, adjustable) is given for 5 seconds (adjustable duration). If the mouse escapes to the safe area within 20 seconds after the light or sound is turned on, it is an active avoidance reaction. If it escapes to the safe area only after the electric shock, it is a passive avoidance reaction. After several training sessions, the mouse can gradually form an active avoidance conditioned response, thereby acquiring memory.

[0094] (2) Formal testing period: During the experiment, the experimental animals were placed in the electric shock area of ​​the shuttle box, and the methods and steps were the same as those of the training period. The number of times the animals were shocked (the number of times they passively avoided) and the number of times they actively avoided were recorded. The first training was the first day, and each mouse was trained 25 times / day, that is, the number of tests set after 24 hours was 25 times, and continued for 4 weeks. After the end of the training, the drug was stopped for one week, and the active avoidance rate was tested to determine the mice's extinction phase reaction.

[0095] Test active avoidance rate (%) = (active avoidance times / total test times) × 100%

[0096] The test results are shown in Table 1:

[0097] Table 1

[0098]

[0099]

[0100] As can be seen from the data in Table 1, mice were continuously given three different doses of the test substance of the present invention by oral gavage for 28 days. The phosphatidylserine in the mouse shuttle box experiment and the high-dose experimental group both showed positive results, and had a certain intelligence-enhancing effect on the mice compared with the negative control group, indicating that the formula nutritional preparation for preventing and intervening in Alzheimer's disease of the present invention has an excellent intelligence-enhancing effect; the mice given each dose of the test substance by oral gavage were in good condition during the experimental training, indicating that the formula nutritional preparation for preventing and intervening in Alzheimer's disease of the present invention is safe and has no adverse effects.

[0101] 2. Prevention and Intervention Effects on Alzheimer's Disease

[0102] 2.1 Experimental subjects

[0103] A total of 175 volunteers with early symptoms of Alzheimer's disease, such as behavioral confusion, aphasia, memory loss, dizziness, and slow movement, were selected for the experiment. Among them, there were 100 males and 75 females, aged 50-85 years old.

[0104] 2.2 Experimental methods

[0105] Volunteers were divided into 7 groups, each with 25 participants. Each group consumed the nutritional formulations for preventing and intervening Alzheimer's disease obtained from the embodiments of the present invention and the comparative examples. The dosage consisted of adding 10g of the nutritional formulations for preventing and intervening Alzheimer's disease to 100ml of 40°C stable water and stirring evenly. The formulations were administered once in the morning and evening for 3 months. The preventive and intervention effects were evaluated, and the intelligence-enhancing effects were determined.

[0106] Markedly effective: The main symptoms have completely disappeared or basically disappeared, the patient is conscious and well-oriented, can answer questions basically correctly, has relatively sensitive reactions, can take care of himself, and can carry out general social activities;

[0107] Effective: The main mental symptoms have been weakened or partially disappeared, the patient can take care of himself and answer questions basically correctly, but the reaction is slow, and there are still some intellectual and personality disorders;

[0108] Ineffective: The main symptoms remain unchanged or continue to develop, the patient cannot take care of himself, answers questions incorrectly, and has dementia.

[0109] The test results are shown in Table 2:

[0110] Table 1

[0111]

[0112]

[0113] It can be seen from the data in Table 1 that the formula nutritional preparations for preventing and intervening in Alzheimer's disease in Examples 1-3 of the present invention have excellent intelligence-enhancing effects; from the comparison of Examples 4 and 5 with Example 1, it can be seen that changing the ratio of lecithin and resveratrol-galactoside or lecithin and phosphatidylserine will affect the loading amount of the phosphatidylserine complex or the sustained-release effect of phosphatidylserine, thereby reducing the intelligence-enhancing effect of the formula nutritional preparation; from the comparison of Example 6 and Example 1, it can be seen that changing the ratio of lactose and β-galactosidase will prevent resveratrol from being released in time and acting on brain cells, thereby slightly reducing the intelligence-enhancing effect of the formula nutritional preparation; from the comparison of Comparative Example 1 and Example 1, it can be seen that if phosphatidylserine is not modified, the intelligence-enhancing effect of the formula nutritional preparation is relatively poor.

[0114] The above embodiments are only for illustrating the technical concept and features of the present invention. Its purpose is to enable people familiar with this technology to understand the content of the present invention and implement it. It cannot be used to limit the scope of protection of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of protection of the present invention.

Claims

1. A nutritional preparation for preventing and intervening in Alzheimer's disease, characterized in that: The invention comprises the following raw materials in parts by weight: 3-6 parts of phosphatidylserine complex, 10-20 parts of refined fish oil, 1-3 parts of grape seed extract, 50-150 parts of medium-chain triglycerides, 1-3 parts of epigallocatechin gallate, 5-10 parts of blueberry powder, 5-10 parts of sea buckthorn powder, 5-10 parts of blackcurrant powder, 2-5 parts of phospholipids, 10-30 parts of xylo-oligosaccharides and 0.5-1 part of B vitamins.

2. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 1, characterized in that: The preparation method of the phosphatidylserine complex comprises the following steps: A1. Add phosphatidylserine to deionized water, add an emulsifier, stir, and homogenize to obtain solution 1; A2. Add lecithin and resveratrol-galactoside to deionized water, stir at high speed at 40-50° C. for 20-30 min to obtain solution 2. Add solution 1 obtained in step A1 to solution 2, stir to obtain a uniform emulsion, and spray dry to obtain a phosphatidylserine complex.

3. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 2, characterized in that: The mass ratio of the lecithin to resveratrol-galactoside is 1:(0.1-0.4).

4. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 2, characterized in that: The mass ratio of the lecithin to phosphatidylserine is 1:(0.2-0.4).

5. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 2, characterized in that: The preparation method of resveratrol-galactoside comprises the following steps: B1. Add lactose to a phosphate buffer solution to obtain a lactose solution. Add resveratrol, β-galactosidase, and the lactose solution to acetone. React at 40-50° C. for 40-60 minutes, then heat to 100° C. and boil for 4-6 minutes to obtain a reaction solution. B2. Centrifuge the reaction solution obtained in step B1 for 15-25 minutes, draw the supernatant, adsorb it with a macroporous adsorption resin, elute it with an ethanol gradient, and freeze-dry it to obtain resveratrol-galactoside.

6. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 5, characterized in that: The mass ratio of the lactose to resveratrol is 1:(0.55-0.65).

7. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 5, characterized in that: The model of the macroporous adsorption resin is XAD-16.

8. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 1, characterized in that: The B vitamins are a combination of B6, B9, and B12, and the mass ratio of the three is 1: (0.25-0.31): (0.001-0.002).

9. The nutritional preparation for preventing and intervening in Alzheimer's disease according to claim 1, characterized in that: The phospholipid is soybean lecithin.

10. A method for preparing the nutritional preparation for preventing and intervening in Alzheimer's disease according to any one of claims 1 to 9, characterized in that: The method comprises the following steps: uniformly mixing phosphatidylserine complex, refined fish oil, grape seed extract, medium chain triglyceride, epigallocatechin gallate, blueberry powder, sea buckthorn powder, black currant powder, phospholipids, oligoxylose and B vitamins.

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