Cell culture bioreactor

Abstract

The invention provides a cell culture bioreactor, and relates to the technical field of cell culture reactors. The reaction mechanism comprises a base, the upper surface of the base is fixedly connected with a reaction tank, the upper surface of the reaction tank is fixedly connected with a sealing cover, the upper surface of the sealing cover is fixedly connected with a servo motor, and the power output end of the servo motor is fixedly connected with a rotating rod; the bottom end of the rotating rod penetrates through the sealing cover and extends into the reaction tank, the rotating rod is rotationally connected with the sealing cover, and the bottom end of the rotating rod is fixedly connected with stirring blades; foam in the reaction tank can be pressed downwards through the pressing-down defoaming unit until the foam is pressed below the liquid level of the nutrient solution in the reaction tank, and at the moment, bubbles generated by stirring the nutrient solution can enter the nutrient solution again to be in contact with the nutrient solution again, so that the bubbles are fused into the nutrient solution, and the nutrient solution is stirred. Therefore, bubbles on the liquid level of the nutrient solution in the reaction tank are eliminated to a certain extent, and the phenomenon of excessive inactivation of biological cells caused by the bubbles is prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture reactors, in particular to a cell culture bioreactor. Background Art

[0002] When biological cells are cultured in vitro, the bioreactor is the key equipment in the entire culture process. It provides a suitable growth environment for the cells, allowing them to proliferate rapidly and form the required biological tissue products. Since animal cells are different from microbial cells in their morphological structure, culture methods, and required mechanical environment, traditional microbial reactors are obviously no longer suitable for large-scale culture of animal cells. Therefore, dedicated biological cell culture reactors are needed. Biological cell culture reactors are mainly composed of stirring mechanisms, monitoring equipment such as temperature and pH value monitoring, reaction tanks, and nutrient solution filling mechanisms.

[0003] In order to increase the degree of binding between biological cells and nutrient solution, or improve the mixing effect of oxygen and nutrient solution, biological cell culture reactors need to use a stirring mechanism to use a motor to drive a stirring rack to stir the nutrient solution inside the reaction tank, so that oxygen can be fully integrated with the nutrient solution, thereby ensuring the normal growth of biological cells. However, due to the large amount of nutrients in the nutrient solution, the concentration of the nutrient solution is high, and a large number of bubbles will form on the surface of the nutrient solution during stirring. Because the bubbles are difficult to be effectively eliminated inside the reaction tank, the existing cell culture bioreactors do not have a bubble elimination component, so when biological cells stay on the bubbles, they are easily inactivated due to insufficient nutrients and oxygen, thereby greatly limiting the high-density, high-expression and high-quality continuous culture effects of biological cells. Summary of the Invention

[0004] In view of this, the present invention provides a cell culture bioreactor to solve the problem that it is difficult to eliminate bubbles in the reaction tank to ensure stirring, so that the bubbles cause biological cells to be inactivated and affect the biological cell culture effect of the biological cell culture reactor.

[0005] The technical solution proposed by the present invention is: a cell culture bioreactor, specifically comprising a reaction mechanism; the reaction mechanism comprises a base, the upper surface of the base is fixedly connected to a reaction tank, the upper surface of the reaction tank is fixedly connected to a sealing cover, the upper surface of the sealing cover is fixedly connected to a servo motor, the power output end of the servo motor is fixedly connected to a rotating rod, the bottom end of the rotating rod passes through the sealing cover and extends to the interior of the reaction tank, the rotating rod is rotatably connected to the sealing cover, the bottom end of the rotating rod is fixedly connected to a stirring blade, the upper surface of the sealing cover is fixedly connected to an oxygen tube, the bottom surface of the reaction tank is fixedly connected to a liquid outlet valve, the liquid outlet of the liquid outlet valve passes through the base and extends to the bottom of the base, and a defoaming mechanism is provided above the sealing cover; The defoaming mechanism includes a downward pressure defoaming unit, which is arranged above the sealing cover and is used to press the foam in the reaction tank down to below the liquid level of the nutrient solution in the reaction tank; The defoaming mechanism further includes a foam absorption unit, which is arranged above the sealing cover and is used to absorb foam on the surface of the nutrient solution in the reaction tank; A liquid adding mechanism is provided on the left side of the foam absorption unit. The liquid adding mechanism is used in conjunction with the defoaming mechanism to reduce the foam generated by splashing when the nutrient solution is added into the reaction tank.

[0006] Furthermore, the downward pressure defoaming unit includes a conical downward pressure cover, which is located inside the reaction tank and is slidably connected to the outside of the rotating rod. The outer surface of the conical downward pressure cover is provided with a plurality of open grooves. The outer surface of the output end of the servo motor is fixedly connected to the first bevel gear, and the first bevel gear is located above the sealing cover. The upper surface of the sealing cover is fixedly connected to two support frames, and the interiors of the two support frames are rotatably connected to a bidirectional screw, and the outer surfaces of the two bidirectional screws are fixedly connected to the second bevel gear. The outer surface of the first bevel gear is meshed with two bevel gear reversing frames, and the outer surfaces of the two bevel gear reversing frames are both aligned with the second bevel gear. The outer surfaces are meshed with each other, and the outer surfaces of the two bevel gear reversing frames are rotatably connected to the bearing seats, the bottom surfaces of the two bearing seats are fixedly connected to the upper surface of the sealing cover, the outer surfaces of the two bidirectional screws are threadedly connected to the moving frames, and the two moving frames are located above the second bevel gear, the inner walls of the two support frames are fixedly connected to the limiting rods, and the two limiting rods are slidably connected to the moving frames, and the inner walls of the two moving frames are fixedly connected to connecting pipes, the bottom ends of the two connecting pipes pass through the sealing cover and the conical down-pressure cover in turn and extend to the bottom of the conical down-pressure cover, the two connecting pipes are slidably connected to the sealing cover, and the outer surfaces of the two connecting pipes are fixedly connected to the conical down-pressure cover.

[0007] Furthermore, the outer surfaces of the two connecting pipes are each sleeved with a sealing frame, the bottom surfaces of the two sealing frames are fixedly connected to the upper surface of the sealing cover, the inner walls of the two sealing frames are fixedly connected to two first sealing rings, and the inner wall of each of the first sealing rings is in contact with the outer surface of the connecting pipe.

[0008] Furthermore, the foam absorption unit includes two extraction cylinders, both of which are arranged above the sealing cover, and the tops of the two extraction cylinders are provided with connecting disks, and the outer surfaces of the two connecting disks are fixedly connected to fixing frames, and the sides of the two fixing frames that are close to each other are fixedly connected to the sides of the two movable frames that are away from each other, and the bottom surfaces of the two connecting disks are fixedly connected to pull rods, and the bottom ends of the two pull rods are fixedly connected to pistons, and the two pistons are slidably connected to the inside of the extraction cylinders, and the bottom surfaces of the two extraction cylinders are fixedly connected to fixed pipes, and the two fixed The outer surface of the fixed pipe is fixedly connected with a liquid inlet pipe, and the other ends of the two liquid inlet pipes are fixedly connected with a connecting hose, and the other ends of the two connecting hoses are fixedly connected with the top of the connecting pipe, and the bottom end of the fixed pipe is fixedly connected with a water suction pipe, and the other end of the water suction pipe passes through the sealing cover and extends to the interior of the reaction tank. An annular pipe is provided below the conical downward pressure cover, and the annular pipe is located inside the reaction tank. Several inlet ports are provided on the outer surface of the annular pipe, and the inner wall of the annular pipe is fixedly connected with a multi-way pipe, and the bottom ends of the two connecting pipes are fixedly connected to the outer surface of the multi-way pipe.

[0009] Furthermore, outer surfaces of the two extraction cylinders are fixedly connected to a stabilizing frame, and bottom surfaces of the two stabilizing frames are fixedly connected to the upper surface of the sealing cover.

[0010] Furthermore, three limiting plates are fixedly connected to the outer surfaces of the two pull rods, and the outer surface of each limiting plate is in contact with the inner wall of the extraction cylinder.

[0011] Furthermore, three fixing blocks are fixedly connected to the outer surface of the annular tube, and the upper surface of each fixing block is fixedly connected to the bottom surface of the conical downward pressure cover.

[0012] Furthermore, the liquid adding mechanism includes two limit frames, the bottom surfaces of the two limit frames are fixedly connected to the upper surface of the sealing cover, the interiors of the two limit frames are rotatably connected to the guide rods through pin shafts, the outer surfaces of the two extraction cylinders are fixedly connected to the liquid adding tubes, the outer surfaces of the two liquid adding tubes are fixedly connected to the liquid adding one-way valves, the inner walls of the two liquid adding tubes are fixedly connected to the first fixing rings, one side surface of the two first fixing rings are fixedly connected to the first springs, the other ends of the two first springs are fixedly connected to the first sealing plugs, the outer surfaces of the two first sealing plugs are sleeved with the first step frames, the outer surfaces of the two first step frames are fixedly connected to the inner walls of the liquid adding tubes, and the other ends of the two first sealing plugs are fixedly connected The lockhole that is formed on the two ends of the lifting pin is formed on the upper end of the lifting pin of the second rim, and the lower end of the lifting pin is fixed with a bolt, and the bolt has a round shank to contact with the bottom end of the lifting pin of the second rim.

[0013] Furthermore, outer surfaces of the two connecting rods are slidably connected with second sealing rings, and the side surfaces of the two second sealing rings close to each other are fixedly connected to ends of the two liquid adding pipes away from each other.

[0014] Furthermore, outer surfaces of the two movable rods are slidably connected to third sealing rings, and side surfaces of the two third sealing rings close to each other are fixedly connected to the outer surface of the fixed tube.

[0015] The cell culture bioreactor provided by the present invention has the following beneficial effects: When the present invention is in use, by providing a downward-pressing defoaming unit, the foam in the reaction tank can be pressed downward until the foam is pressed below the liquid level of the nutrient solution in the reaction tank. At this time, the bubbles generated by the stirring of the nutrient solution in the biological cell culture reactor will re-enter the nutrient solution and come into contact with the nutrient solution again, so that the bubbles are integrated into the nutrient solution, thereby eliminating the bubbles on the liquid level of the nutrient solution in the reaction tank to a certain extent, and preventing the excessive inactivation of biological cells caused by the bubbles. By setting up a foam absorption unit, bubbles on the liquid surface of the reaction tank and part of the nutrient solution can be sucked into the extraction tube, so that the bubbles and the nutrient solution are fused in the extraction tube, and then the sucked bubbles and nutrient solution are re-discharged from the extraction tube into the reaction tank. In the process of the bubbles and nutrient solution being discharged into the reaction tank, the bubbles will be forced to further fuse with the nutrient solution under pressure, so that the bubbles in the reaction tank can be further eliminated, so that the cell culture bioreactor has a good bubble elimination function, which is convenient for biological cell culture to achieve high-density, high-expression and high-quality continuous culture effects; By setting up a liquid adding mechanism, the flow rate and impact of the nutrient solution when entering the reaction tank can be reduced, making the nutrient solution more gentle and even when added, and preventing the nutrient solution from generating a large amount of foam due to excessive impact caused by excessive concentration of water flow, thereby further reducing the impact of foam on biological cell culture. In addition, the liquid adding mechanism can also flush out the foam remaining in the foam absorption unit, preventing excessive bubbles from remaining in the internal pipe of the foam absorption unit and affecting the survival of biological cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings of the embodiments are briefly introduced below.

[0017] The drawings described below only relate to some embodiments of the present invention, but are not intended to limit the present invention.

[0018] In the attached figure: Figure 1 Shows a schematic structural diagram of the present invention as a whole; Figure 2 A schematic structural diagram of a cross-section of a reaction tank of the present invention is shown; Figure 3 A schematic cross-sectional view of the conical downward pressure cover of the present invention is shown; Figure 4 Shows a schematic structural diagram of the support frame of the present invention; Figure 5 Shows a schematic structural diagram of the connecting pipe of the present invention; Figure 6 A schematic structural diagram of a cross-section of the extraction tube of the present invention is shown; Figure 7 A schematic structural diagram of a cross-section of a fixed tube according to the present invention is shown; Figure 8 A schematic structural diagram of a cross-section of the liquid adding tube of the present invention is shown.

[0019] Reference Signs List 1. Reaction mechanism; 11. Base; 12. Reaction tank; 13. Sealing cover; 14. Servo motor; 15. Rotating rod; 16. Stirring blade; 17. Oxygen tube; 18. Liquid outlet valve; 2. Defoaming mechanism; 21. Down-pressure defoaming unit; 2101. Conical down-pressure cover; 2102. Opening groove; 2103. First bevel gear; 2104. Support frame; 2105. Bidirectional screw; 2106. Second bevel gear; 2107. Bevel gear reversing frame; 2108. Bearing seat; 2109. Moving frame; 2110. Connecting pipe; 2111. Sealing frame; 2112. First sealing ring; 2113. Limiting rod; 22. Foam absorption unit; 2201. Extraction cylinder; 2202. Connecting plate; 2203. Fixed frame; 2204. Pull rod; 2205. Piston ;2206, fixed tube;2207, liquid inlet pipe;2208, connecting hose;2209, annular tube;2210, inlet port;2211, multi-way tube;2212, stabilizing frame;2213, limit plate;2214, fixed block;2215, water suction pipe;3, liquid adding mechanism;301, limit frame;302, guide rod;303, liquid adding pipe;304, liquid adding one-way valve;305, first fixed ring;306, first spring;307, first stepped frame;308, first sealing plug;309, connecting rod;310, second stepped frame;311, second sealing plug;312, second spring;313, second fixed ring;314, movable rod;315, sliding cylinder;316, fixed rod;317, second sealing ring;318, third sealing ring. DETAILED DESCRIPTION

[0020] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] For ease of description, X is calibrated as the left-right direction, and Y is calibrated as the front-back direction.

[0022] Please refer to Figures 1 to 4 , Example 1: The present invention provides a cell culture bioreactor, comprising a reaction mechanism 1; the reaction mechanism 1 comprises a base 11, the upper surface of the base 11 is fixedly connected to a reaction tank 12, the upper surface of the reaction tank 12 is fixedly connected to a sealing cover 13, the upper surface of the sealing cover 13 is fixedly connected to a servo motor 14, the power output end of the servo motor 14 is fixedly connected to a rotating rod 15, the bottom end of the rotating rod 15 passes through the sealing cover 13 and extends into the interior of the reaction tank 12, the rotating rod 15 is rotatably connected to the sealing cover 13, the bottom end of the rotating rod 15 is fixedly connected to a stirring blade 16, the upper surface of the sealing cover 13 is fixedly connected to an oxygen tube 17, the bottom surface of the reaction tank 12 is fixedly connected to a liquid outlet valve 18, the liquid outlet of the liquid outlet valve 18 passes through the base 11 and extends to the bottom of the base 11, and a defoaming mechanism 2 is provided above the sealing cover 13; The defoaming mechanism 2 includes a downward-pressing defoaming unit 21, which is disposed above the sealing cover 13 and is used to press the foam in the reaction tank 12 down to below the liquid level of the nutrient solution in the reaction tank 12; The defoaming mechanism 2 further includes a foam absorption unit 22, which is disposed above the sealing cover 13 and is used to absorb the foam on the surface of the nutrient solution in the reaction tank 12; A liquid adding mechanism 3 is provided on the left side of the foam absorption unit 22 . The liquid adding mechanism 3 is used in conjunction with the defoaming mechanism 2 . The liquid adding mechanism 3 is used to reduce the foam generated by splashing when the nutrient solution is added into the reaction tank 12 .

[0023] Preferably, the downward pressure defoaming unit 21 includes a conical downward pressure cover 2101, which is located inside the reaction tank 12, and the conical downward pressure cover 2101 is slidably connected to the outside of the rotating rod 15. The outer surface of the conical downward pressure cover 2101 is provided with a plurality of open grooves 2102. The outer surface of the output end of the servo motor 14 is fixedly connected to the first bevel gear 2103, and the first bevel gear 2103 is located above the sealing cover 13. The upper surface of the sealing cover 13 is fixedly connected to two support frames 2104, and the interiors of the two support frames 2104 are both rotatably connected to the bidirectional screws 2105. The outer surfaces of the two bidirectional screws 2105 are both fixedly connected to the second bevel gear 2106. The outer surface of the first bevel gear 2103 is meshed with two bevel gear reversing frames 2107, and the outer surfaces of the two bevel gear reversing frames 2107 are meshed with the outer surface of the second bevel gear 2106. The outer surfaces of the two bevel gear reversing frames 2107 are rotatably connected to the bearing seats 2108, and the bottom surfaces of the two bearing seats 2108 are fixedly connected to the upper surface of the sealing cover 13. The outer surfaces of the two bidirectional screws 2105 are threadedly connected to the movable frames 2109. The two movable frames 2109 are located above the second bevel gear 2106. The inner walls of the two support frames 2104 are fixedly connected to the limiting rods 2113, and the two limiting rods 2113 are slidably connected to the movable frames 2109. The inner walls of the two movable frames 2109 are fixedly connected to the connecting pipes 2110. The bottom ends of the two connecting pipes 2110 pass through the sealing cover 13 and the conical downward pressure cover 2101 in sequence and extend to the bottom of the conical downward pressure cover 2101. The two connecting pipes 2110 are slidably connected to the sealing cover 13, and the outer surfaces of the two connecting pipes 2110 are fixedly connected to the conical downward pressure cover 2101.

[0024] By setting up the downward pressure defoaming unit 21, the foam in the reaction tank 12 can be pressed downward until the foam is pressed below the liquid level of the nutrient solution in the reaction tank 12. At this time, the bubbles generated by the biological cell culture reactor stirring the nutrient solution will re-enter the nutrient solution and contact the nutrient solution again, so that the bubbles are integrated into the nutrient solution, and then the bubbles on the liquid level of the nutrient solution in the reaction tank 12 are eliminated to a certain extent, preventing the bubbles from causing excessive inactivation of biological cells.

[0025] Preferably, the outer surfaces of the two connecting tubes 2110 are both sleeved with sealing frames 2111, the bottom surfaces of the two sealing frames 2111 are fixedly connected to the upper surface of the sealing cover 13, and the inner walls of the two sealing frames 2111 are both fixedly connected with two first sealing rings 2112, and the inner wall of each first sealing ring 2112 is in contact with the outer surface of the connecting tube 2110.

[0026] By cooperating with the two first sealing rings 2112 through the sealing frame 2111, the sealing between the connecting tube 2110 and the sealing cover 13 can be increased without affecting the up and down movement of the connecting tube 2110, thereby preventing leakage from occurring in the gap between the connecting tube 2110 and the sealing cover 13, which may cause contamination of the biological cells inside the reaction tank 12.

[0027] On the basis of embodiment 1, embodiment 2, as Figures 3 to 6 As shown: A cell culture bioreactor, the foam absorption unit 22 includes two extraction cylinders 2201, the two extraction cylinders 2201 are both arranged above the sealing cover 13, the tops of the two extraction cylinders 2201 are both provided with connecting disks 2202, the outer surfaces of the two connecting disks 2202 are fixedly connected to the fixing frames 2203, the sides of the two fixing frames 2203 close to each other are respectively fixedly connected to the sides of the two movable frames 2109 away from each other, the bottom surfaces of the two connecting disks 2202 are both fixedly connected to the pull rods 2204, the bottom ends of the two pull rods 2204 are both fixedly connected to the pistons 2205, the two pistons 2205 are both slidably connected to the inside of the extraction cylinders 2201, the bottom surfaces of the two extraction cylinders 2201 are both fixedly connected to the fixed pipes 2206, and the two fixed pipes 2207 are fixedly connected to the fixed pipes 2208. The outer surface of 206 is fixedly connected with a liquid inlet pipe 2207, and the other ends of the two liquid inlet pipes 2207 are fixedly connected with a connecting hose 2208. The other ends of the two connecting hoses 2208 are fixedly connected to the top of the connecting pipe 2110. The bottom end of the fixed pipe 2206 is fixedly connected with a water suction pipe 2215. The other end of the water suction pipe 2215 passes through the sealing cover 13 and extends to the interior of the reaction tank 12. An annular pipe 2209 is arranged below the conical downward pressure cover 2101. The annular pipe 2209 is located inside the reaction tank 12. Several inlet ports 2210 are opened on the outer surface of the annular pipe 2209. The inner wall of the annular pipe 2209 is fixedly connected with a multi-way pipe 2211. The bottom ends of the two connecting pipes 2110 are fixedly connected to the outer surface of the multi-way pipe 2211.

[0028] By setting up the foam absorption unit 22, the bubbles on the liquid surface of the reaction tank 12 and part of the nutrient solution can be sucked into the extraction cylinder 2201, so that the bubbles and the nutrient solution are fused in the extraction cylinder 2201, and then the sucked bubbles and nutrient solution are re-discharged into the reaction tank 12 from the extraction cylinder 2201. In the process of discharging the bubbles and nutrient solution into the reaction tank 12, the bubbles will be forced to further fuse with the nutrient solution under pressure, so that the bubbles in the reaction tank 12 can be further eliminated, so that the cell culture bioreactor has a good bubble elimination function, which is convenient for biological cell culture to achieve high-density, high-expression and high-quality continuous culture effects.

[0029] Preferably, the outer surfaces of the two extraction cylinders 2201 are fixedly connected to a stabilizing frame 2212 , and the bottom surfaces of the two stabilizing frames 2212 are fixedly connected to the upper surface of the sealing cover 13 .

[0030] The stabilizing frame 2212 can stabilize the position of the extraction tube 2201 so that the extraction tube 2201 can be firmly fixed on the sealing cover 13, thereby increasing the stability and reliability of the extraction tube 2201 during use.

[0031] Preferably, three limiting plates 2213 are fixedly connected to the outer surfaces of the two pull rods 2204 , and the outer surface of each limiting plate 2213 is in contact with the inner wall of the extraction cylinder 2201 .

[0032] The limiting plate 2213 can increase the stability of the pull rod 2204 when it moves up and down inside the extraction cylinder 2201, prevent the pull rod 2204 from deflecting when it moves up and down, and improve the reliability of the pull rod 2204 when in use.

[0033] Preferably, three fixing blocks 2214 are fixedly connected to the outer surface of the annular tube 2209 , and the upper surface of each fixing block 2214 is fixedly connected to the bottom surface of the conical downward pressure cover 2101 .

[0034] The position of the annular tube 2209 can be fixed by the fixing block 2214 so that the annular tube 2209 can be firmly fixed on the conical downward pressure cover 2101, thereby increasing the firmness of the annular tube 2209 when in use.

[0035] On the basis of the second embodiment, the third embodiment, as shown in FIG. Figures 6 to 8 As shown: A cell culture bioreactor, the liquid adding mechanism 3 includes two limiting frames 301, the bottom surfaces of the two limiting frames 301 are fixedly connected to the upper surface of the sealing cover 13, the interiors of the two limiting frames 301 are rotatably connected to the guide rod 302 through a pin shaft, the outer surfaces of the two extraction cylinders 2201 are fixedly connected to the liquid adding tube 303, the outer surfaces of the two liquid adding tubes 303 are fixedly connected to the liquid adding one-way valve 304, the inner walls of the two liquid adding tubes 303 are fixedly connected to the first fixing ring 305, one side of the two first fixing rings 305 are fixedly connected to the first spring 306, the other ends of the two first springs 306 are fixedly connected to the first sealing plug 308, the outer surfaces of the two first sealing plugs 308 are sleeved with a first step frame 307, the outer surfaces of the two first step frames 307 are fixedly connected to the inner wall of the liquid adding tube 303, the other ends of the two first sealing plugs 308 are fixedly connected to the connecting rod 309, and the other ends of the two connecting rods 309 are fixedly connected. When the lock is unlocked, the lock 314 is unlocked, and the lock 314 is unlocked, so that the lock 314 can be unlocked. When the lock 314 is unlocked, the lock 314 can be unlocked.

[0036] By setting up the liquid adding mechanism 3, the flow rate and impact of the nutrient solution when entering the reaction tank 12 can be reduced, so that the nutrient solution can be added more gently and evenly, and the nutrient solution is not likely to produce a large amount of foam due to excessive impact force caused by excessive concentration of water flow, thereby further reducing the impact of foam on biological cell culture. In addition, the liquid adding mechanism 3 can also flush out the foam remaining in the foam absorption unit 22, preventing excessive bubbles from remaining in the internal pipe of the foam absorption unit 22 and affecting the survival of biological cells.

[0037] Preferably, the outer surfaces of the two connecting rods 309 are slidably connected with second sealing rings 317 , and the side surfaces of the two second sealing rings 317 close to each other are fixedly connected to the ends of the two liquid adding tubes 303 away from each other.

[0038] The second sealing ring 317 can increase the sealing between the connecting rod 309 and the liquid adding tube 303, making it difficult for liquid to leak out through the gap, thereby improving the coordination effect between the connecting rod 309 and the liquid adding tube 303.

[0039] Preferably, the outer surfaces of the two movable rods 314 are slidably connected to the third sealing rings 318 , and the side surfaces of the two third sealing rings 318 close to each other are fixedly connected to the outer surface of the fixed tube 2206 .

[0040] The third sealing ring 318 can seal the connection between the movable rod 314 and the fixed tube 2206 to a certain extent, so that the movable rod 314 will not cause leakage in the fixed tube 2206 when it moves, thereby improving the sealing effect of the fixed tube 2206 and the movable rod 314.

[0041] Working principle: first connect the servo motor 14 with the external power supply and the controller. When the nutrient solution and the biological cells need to be fully mixed and stirred, the power provided by the servo motor 14 is used to cooperate with the sealing cover 13 to drive the rotating rod 15 and the stirring blade 16 to rotate, so as to realize the stirring of the nutrient solution. When the nutrient solution inside the reaction tank 12 is stirred, the rotation of the servo motor 14 will also drive the first bevel gear 2103 to rotate. The engagement of the first bevel gear 2103 with the bevel gear reversing frame 2107 and the limitation of the bevel gear reversing frame 2107 by the bearing seat 2108 can drive the second bevel gear 2106 to rotate, and then the bidirectional screw 2105 can be driven to rotate under the limitation of the support frame 2104. The special bidirectional thread on the bidirectional screw 2105 can drive the movable frame 2109 to move up and down under the limitation of the limiting rod 2113. When the movable frame 2109 moves up and down, it can be sealed by the sealing frame 2111 and the first sealing ring 2112. The connecting pipe 2110 drives the conical downward pressure cover 2101 to move up and down. When the conical downward pressure cover 2101 moves up and down, the foam in the reaction tank 12 is pressed downward until the foam is pressed below the liquid level of the nutrient solution in the reaction tank 12. At this time, the bubbles generated by the biological cell culture reactor stirring the nutrient solution will re-enter the nutrient solution and contact the nutrient solution again, so that the bubbles are integrated into the nutrient solution, thereby eliminating the bubbles on the liquid level of the nutrient solution in the reaction tank 12 to a certain extent, and preventing the bubbles from causing excessive inactivation of biological cells. Moreover, due to the through hole on the conical downward pressure cover 2101, when the conical downward pressure frame moves upward, the nutrient solution will flow back into the reaction tank 12 through the through hole and the slope of the conical downward pressure cover 2101 itself, and the design of the open groove 2102 allows the conical downward pressure cover 2101 to move up and down smoothly while the sensor probe or pipeline is located in the open groove 2102, thereby not affecting the normal use of other sensor probes or pipelines in the cell culture bioreactor; When the movable frame 2109 moves up and down, it will also cooperate with the fixed frame 2203 to drive the connecting plate 2202 and the pull rod 2204 to move up and down, thereby driving the piston 2205 to move inside the extraction cylinder 2201. When the piston 2205 moves upward, a negative pressure environment will be formed inside the extraction cylinder 2201 and suction will be generated. Then, part of the nutrient solution in the reaction tank 12 will be sucked into the extraction cylinder 2201 through the fixed pipe 2206 and the water suction pipe 2215. At the same time, the fixed pipe 2206 is used to cooperate with the suction pipe 2215 to suck the nutrient solution into the extraction cylinder 2201. The liquid pipe 2207 and the connecting hose 2208 can generate suction inside the annular tube 2209 through the connecting pipe 2110 and the multi-way pipe 2211 without affecting the up and down movement of the mobile frame 2109, so that part of the nutrient solution is sucked into the extraction cylinder 2201 from the inlet 2210. When the annular tube 2209 is moved upward to the liquid surface of the nutrient solution in the reaction tank 12 by the conical downward pressure cover 2101 under the cooperation of the fixed block 2214, the bubbles can be destroyed by the suction. The bubbles are further eliminated by rupture, and some bubbles enter the extraction cylinder 2201 through the inlet 2210, the annular tube 2209, the multi-way tube 2211, the connecting tube 2110, the connecting hose 2208, the liquid inlet tube 2207 and the fixed tube 2206, and are preliminarily mixed with the nutrient solution entering the extraction cylinder 2201 through the water suction pipe 2215 in the extraction cylinder 2201. When the piston 2205 moves downward, the nutrient solution and bubbles in the extraction cylinder 2201 are pushed into the water suction pipe 2215 through the fixed tube 2206. Since the diameter of the water suction pipe 2215 is smaller than that of the extraction cylinder 2201, the bubbles are forced to merge with the nutrient solution under pressure and finally re-enter the nutrient solution in the reaction tank 12 from the water suction pipe 2215 and merge with the nutrient solution again, so that the bubbles in the reaction tank 12 can be further eliminated, so that the cell culture bioreactor has a good bubble elimination function, which is convenient for biological cell culture to achieve high density, high expression and high-quality continuous culture effects; When the piston 2205 moves upward, the second spring 312 will shrink under pressure, so that the second sealing plug 311 cooperates with the second fixing ring 313 to separate from the second ladder frame 310, allowing the nutrient solution and bubbles to smoothly enter the extraction cylinder 2201 through the gap between the second ladder frame 310 and the second sealing plug 311. Moreover, since the suction force is generated by the extraction cylinder 2201 and the suction force is upward, the nutrient solution will not generate a rightward thrust on the second sealing plug 311 during the process of entering the extraction cylinder 2201 through the suction pipe 2215, and will not affect the normal opening of the second sealing plug 311. When the piston 2205 moves downward, the pressure direction is opposite, so that the second sealing plug 311 is tightly fitted with the second ladder frame 310, and the second sealing plug 311 is opened. This prevents the nutrient solution and bubbles inside the extraction cylinder 2201 from flowing back through the liquid inlet pipe 2207, and allows the nutrient solution and bubbles to enter the reaction tank 12 only through the fixed pipe 2206 and the water suction pipe 2215, ensuring the normal operation of the bubble elimination work. After the stirring of the nutrient solution inside the reaction tank 12 is completed, or nutrient solution needs to be added again, it is only necessary to send the nutrient solution into the liquid adding pipe 303 through the liquid adding one-way valve 304. As the pressure increases, the nutrient solution will push the first sealing plug 308 to disengage from the first stepped frame 307, and force the first spring 306 to contract under the fixation of the first fixing ring 305. At this time, the nutrient solution will flow into the extraction cylinder 2201 through the gap between the first sealing plug 308 and the first stepped frame 307, and through the fixed pipe When the first sealing plug 308 is separated from the first ladder frame 307, the connecting rod 309 is pushed to slide in the liquid adding tube 303, and then the guide rod 302 is limited by the limiting frame 301, which can push the guide rod 302 to swing clockwise along the pin connection point with the limiting frame 301. When the guide rod 302 swings clockwise, the sliding cylinder 315 and the movable rod 314 are pulled to the left through the gap on the hinge point between the guide rod 302 and the fixed rod 316 through the fixed rod 316, thereby pulling the second sealing plug 311 out of the second ladder frame 310, so that the nutrient solution entering from the liquid adding tube 303 can pass through the fixed tube 2206 and the liquid inlet pipe 220 The connecting hose 2208, the connecting pipe 2110, the multi-way pipe 2211 and the annular pipe 2209 flow into the reaction tank 12 from the inlet 2210, flushing out the bubbles remaining in the fixed pipe 2206, the liquid inlet pipe 2207, the connecting hose 2208, the connecting pipe 2110, the multi-way pipe 2211 and the annular pipe 2209, so that the bubbles are merged with the nutrient solution in the reaction tank 12 again. Finally, when the piston 2205 moves upward to suck the nutrient solution in the reaction tank 12 into the extraction cylinder 2201, forcing the second sealing plug 311 to disengage from the second stepped frame 310, it will push the movable rod 314 to slide inside the sliding cylinder 315, without pushing the guide rod 302 to swing counterclockwise in the opposite direction, thereby preventing the nutrient solution from flowing back through the liquid adding pipe 303.

[0042] In this article, there are several points to note: 1. The drawings of the embodiments of the present invention only relate to the structures related to the embodiments of the present invention. Other structures may refer to conventional designs.

[0043] 2. In the absence of conflict, the embodiments of the present invention and the features therein may be combined with each other to form new embodiments.

[0044] The above are merely specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A cell culture bioreactor, comprising a reaction mechanism (1); characterized in that: The reaction mechanism (1) comprises a base (11), the upper surface of the base (11) is fixedly connected to a reaction tank (12), the upper surface of the reaction tank (12) is fixedly connected to a sealing cover (13), the upper surface of the sealing cover (13) is fixedly connected to a servo motor (14), the power output end of the servo motor (14) is fixedly connected to a rotating rod (15), the bottom end of the rotating rod (15) passes through the sealing cover (13) and extends to the interior of the reaction tank (12), the rotating rod (15) is rotatably connected to the sealing cover (13), the bottom end of the rotating rod (15) is fixedly connected to a stirring blade (16), the upper surface of the sealing cover (13) is fixedly connected to an oxygen pipe (17), the bottom surface of the reaction tank (12) is fixedly connected to a liquid outlet valve (18), the liquid outlet of the liquid outlet valve (18) passes through the base (11) and extends to the bottom of the base (11), and a defoaming mechanism (2) is provided above the sealing cover (13); The defoaming mechanism (2) comprises a downward-pressing defoaming unit (21), which is arranged above the sealing cover (13) and is used to press the foam in the reaction tank (12) downward to below the liquid level of the nutrient solution in the reaction tank (12); The defoaming mechanism (2) further comprises a foam absorption unit (22), the foam absorption unit (22) being arranged above the sealing cover (13), and the foam absorption unit (22) being used to absorb foam on the surface of the nutrient solution in the reaction tank (12); A liquid adding mechanism (3) is provided on the left side of the foam absorption unit (22). The liquid adding mechanism (3) is used in conjunction with the defoaming mechanism (2). The liquid adding mechanism (3) is used to reduce foam generated by splashing when the nutrient solution is added to the interior of the reaction tank (12).

2. A cell culture bioreactor according to claim 1, characterized in that: The downward pressure defoaming unit (21) includes a conical downward pressure cover (2101), the conical downward pressure cover (2101) is located inside the reaction tank (12), the conical downward pressure cover (2101) is slidably connected to the outside of the rotating rod (15), the outer surface of the conical downward pressure cover (2101) is provided with a plurality of open grooves (2102), the outer surface of the output end of the servo motor (14) is fixedly connected with a first bevel gear (2103), the first bevel gear (2103) is located above the sealing cover (13), and the The upper surface of the sealing cover (13) is fixedly connected to two support frames (2104), the interiors of the two support frames (2104) are rotatably connected to bidirectional screws (2105), the outer surfaces of the two bidirectional screws (2105) are fixedly connected to the second bevel gear (2106), the outer surface of the first bevel gear (2103) is meshedly connected to two bevel gear reversing frames (2107), and the outer surfaces of the two bevel gear reversing frames (2107) are meshed with the outer surface of the second bevel gear (2106). The outer surfaces of the two bevel gear reversing frames (2107) are rotatably connected to the bearing seats (2108), the bottom surfaces of the two bearing seats (2108) are fixedly connected to the upper surface of the sealing cover (13), the outer surfaces of the two bidirectional screws (2105) are threadedly connected to the moving frames (2109), the two moving frames (2109) are located above the second bevel gear (2106), the inner walls of the two support frames (2104) are fixedly connected to the limiting rods (2113), the two limiting rods (2113) are both slidably connected to the movable frame (2109), the inner walls of the two movable frames (2109) are fixedly connected with connecting tubes (2110), the bottom ends of the two connecting tubes (2110) pass through the sealing cover (13) and the conical downward pressure cover (2101) in sequence and extend to the bottom of the conical downward pressure cover (2101), the two connecting tubes (2110) are both slidably connected to the sealing cover (13), and the outer surfaces of the two connecting tubes (2110) are fixedly connected to the conical downward pressure cover (2101).

3. A cell culture bioreactor according to claim 2, characterized in that: The outer surfaces of the two connecting tubes (2110) are both sleeved with sealing frames (2111), the bottom surfaces of the two sealing frames (2111) are both fixedly connected to the upper surface of the sealing cover (13), and the inner walls of the two sealing frames (2111) are both fixedly connected to two first sealing rings (2112), and the inner wall of each of the first sealing rings (2112) is in contact with the outer surface of the connecting tube (2110).

4. A cell culture bioreactor according to claim 2, characterized in that: The foam absorption unit (22) includes two extraction cylinders (2201), both of which are arranged above the sealing cover (13), and the tops of the two extraction cylinders (2201) are provided with connecting plates (2202), and the outer surfaces of the two connecting plates (2202) are fixedly connected to the fixing frames (2203), and the sides of the two fixing frames (2203) that are close to each other are fixedly connected to the sides of the two movable frames (2109) that are away from each other, and the bottom surfaces of the two connecting plates (2202) are fixedly connected to the pull rods (2204), and the bottom ends of the two pull rods (2204) are fixedly connected to the pistons (2205), and the two pistons (2205) are slidably connected to the inside of the extraction cylinder (2201), and the bottom surfaces of the two extraction cylinders (2201) are fixedly connected to the fixing pipes (2206), and the outer surfaces of the two fixing pipes (2206) are fixedly connected to the fixing pipes (2206). The surfaces are fixedly connected with a liquid inlet pipe (2207), the other ends of the two liquid inlet pipes (2207) are fixedly connected with a connecting hose (2208), the other ends of the two connecting hoses (2208) are fixedly connected with the top of the connecting pipe (2110), the bottom end of the fixed pipe (2206) is fixedly connected with a water suction pipe (2215), the other end of the water suction pipe (2215) passes through the sealing cover (13) and extends to the interior of the reaction tank (12), an annular pipe (2209) is provided below the conical downward pressure cover (2101), the annular pipe (2209) is located inside the reaction tank (12), a plurality of inlet ports (2210) are opened on the outer surface of the annular pipe (2209), the inner wall of the annular pipe (2209) is fixedly connected with a multi-way pipe (2211), and the bottom ends of the two connecting pipes (2110) are fixedly connected with the outer surface of the multi-way pipe (2211).

5. A cell culture bioreactor according to claim 4, characterized in that: The outer surfaces of the two extraction cylinders (2201) are fixedly connected to a stabilizing frame (2212), and the bottom surfaces of the two stabilizing frames (2212) are fixedly connected to the upper surface of the sealing cover (13).

6. A cell culture bioreactor according to claim 4, characterized in that: The outer surfaces of the two pull rods (2204) are fixedly connected to three limit plates (2213), and the outer surface of each limit plate (2213) is in contact with the inner wall of the extraction cylinder (2201).

7. A cell culture bioreactor according to claim 4, characterized in that: Three fixing blocks (2214) are fixedly connected to the outer surface of the annular tube (2209), and the upper surface of each fixing block (2214) is fixedly connected to the bottom surface of the conical downward pressure cover (2101).

8. A cell culture bioreactor according to claim 4, characterized in that: The liquid adding mechanism (3) comprises two limiting frames (301), the bottom surfaces of the two limiting frames (301) are fixedly connected to the upper surface of the sealing cover (13), the interiors of the two limiting frames (301) are rotatably connected to the guide rod (302) via a pin shaft, the outer surfaces of the two extraction cylinders (2201) are fixedly connected to the liquid adding pipe (303), the outer surfaces of the two liquid adding pipes (303) are fixedly connected to the liquid adding one-way valve (304), and the inner walls of the two liquid adding pipes (303) are fixedly connected to the first fixed Ring (305), one side of the two first fixing rings (305) is fixedly connected to the first spring (306), the other end of the two first springs (306) is fixedly connected to the first sealing plug (308), the outer surface of the two first sealing plugs (308) is sleeved with a first step frame (307), the outer surface of the two first step frames (307) is fixedly connected to the inner wall of the liquid adding tube (303), the other end of the two first sealing plugs (308) is fixedly connected to the connecting rod (309), the two The other ends of the connecting rods (309) pass through the liquid adding tube (303) and are movably hinged to the top of the guide rod (302). The inner walls of the two liquid inlet tubes (2207) are fixedly connected to the second step frame (310). The interiors of the two second step frames (310) are clamped with second sealing plugs (311). The other ends of the two second sealing plugs (311) are fixedly connected to the second spring (312). The other ends of the two second springs (312) are fixedly connected to the second fixing ring (313). The outer surfaces of the second fixing rings (313) are fixedly connected to the inner wall of the liquid inlet pipe (2207), the other ends of the two second sealing plugs (311) are fixedly connected to the movable rod (314), the bottom ends of the two guide rods (302) are movably hinged to the fixing rod (316) through the pin shaft, the other ends of the two fixing rods (316) are fixedly connected to the sliding cylinder (315), and the other ends of the two movable rods (314) pass through the fixing tube (2206) and are slidably connected to the inside of the sliding cylinder (315).

9. A cell culture bioreactor according to claim 8, characterized in that: The outer surfaces of the two connecting rods (309) are both slidably connected to second sealing rings (317), and the side surfaces of the two second sealing rings (317) that are close to each other are fixedly connected to the ends of the two liquid adding pipes (303) that are away from each other.

10. The cell culture bioreactor according to claim 8, characterized in that: The outer surfaces of the two movable rods (314) are both slidably connected to a third sealing ring (318), and the side surfaces of the two third sealing rings (318) that are close to each other are both fixedly connected to the outer surface of the fixed tube (2206).

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