Pretreatment device for gene detection

Abstract

The invention discloses a gene detection pretreatment device which comprises a support, a cleaning container is mounted at the top end of the support, a shifting plate is rotatably arranged on the inner wall of the cleaning container, a rotating shaft is fixedly mounted on the side face of the shifting plate, and the rotating shaft extends into the inner wall of the cleaning container and rotatably sleeves the inner wall of the cleaning container; a guide face, a groove face and a bent face are arranged on the track of the cross section of the inner wall of the bottom end of the cleaning container. Through the arrangement of the shifting plate, the guide surface, the groove surface and the bending surface, the tissue sample is driven to roll towards the groove surface and the bending surface under the action of flowing water by virtue of mutual cooperation of the shifting plate, the guide surface, the groove surface and the bending surface, and the tissue sample is gradually contacted with a plurality of arc-shaped contact plates on the surface of the bending surface; at the moment, when the tissue sample is in contact with the multiple arc-shaped contact plates from bottom to top in sequence, rolling friction can be fully performed between the tissue sample and the rough surfaces of the arc-shaped contact plates, so that impurities adhered to the surfaces of the tissue sample are fully removed under the cleaning cooperation of flowing water.

Description

Technical Field

[0001] The present invention relates to the technical field of sample preprocessing, and more specifically, to a preprocessing device for gene detection. Background Art

[0002] Genetic testing is a technology that detects DNA through blood, other body fluids, or cells. It is a method of taking peripheral venous blood or other tissue cells of the person being tested, amplifying their genetic information, and then using specific equipment to detect the DNA molecular information in the cells of the person being tested, analyzing the gene types and gene defects it contains and whether its expression function is normal. This method enables people to understand their own genetic information, identify the cause of the disease or predict the risk of developing a certain disease. Before testing the tissue cell sample, pretreatment is required, that is, without destroying the cell structure of the sample to be tested, appropriate cleaning methods are used to minimize the interfering components of impurities such as mucus and blood stains on the surface of the tissue sample, so as to improve the sensitivity and specificity of the test, thereby increasing the accuracy of the test.

[0003] Currently, during pre-treatment and cleaning, tissue samples are generally clamped with tweezers, placed in sterile water or pure water, and stirred back and forth for washing. However, during this process, due to the limited force of stirring and washing, some components with strong adhesion to the surface of the tissue sample, such as tissue mucus and blood stains, cannot be fully cleaned and removed. At the same time, due to the presence of a certain contact area dead angle when the tweezers and the tissue sample are in contact and clamped, the current cleaning method cannot fully clean the surface of the tissue sample, which brings certain obstacles to the subsequent detection procedures and reduces the efficiency and accuracy of subsequent detection. Summary of the Invention

[0004] To overcome the aforementioned shortcomings of the prior art, embodiments of the present invention provide a pretreatment device for genetic testing. By providing a paddle, a guide surface, a grooved surface, and a curved surface, the device utilizes the interaction of these components to drive a tissue sample toward the grooved and curved surfaces under the action of flowing water, causing the tissue sample to roll toward the grooved and curved surfaces, gradually contacting the multiple curved contact plates on the curved surface. As the tissue sample gradually contacts the multiple curved contact plates from bottom to top, the rough surfaces of the curved contact plates provide sufficient rolling friction with the tissue sample, thereby fully removing impurities adhering to the surface of the tissue sample, thereby addressing the aforementioned problems raised in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solutions: a pretreatment device for genetic testing, comprising a bracket, a cleaning container mounted on the top of the bracket, a toggle plate rotatably mounted on the inner wall of the cleaning container, a rotating shaft fixedly mounted on the side of the toggle plate, the rotating shaft extending into the inner wall of the cleaning container and rotatably fitted therewith;

[0006] The cross-sectional trajectory of the inner wall of the bottom end of the cleaning container is provided with three sections of trajectories: a guide surface, a groove surface and a curved surface, and the guide surface, the groove surface and the curved surface are distributed in sequence from left to right, the guide surface is a straight surface trajectory as a whole, the overall trajectory of the groove surface increases in sequence from left to right, the overall curvature of the curved surface is greater than the overall curvature of the groove surface, and the bending direction of the curved surface is toward one side of the groove surface, among the guide surface, the groove surface and the curved surface, the horizontal height of the groove surface is the lowest, and the toggle plate is located at a position obliquely above the groove surface;

[0007] A convex surface and a curved surface are respectively provided on the left and right sides of the bottom end of the toggle plate. One side of the bottom end of the convex surface is in contact with the surface of the guide surface. A circular arc surface is provided as a transition between the convex surface and the curved surface. The overall curvature of the convex surface is greater than the overall curvature of the curved surface.

[0008] The inner wall of the curved surface is equidistantly provided with a plurality of arc-shaped touch pads from bottom to top, and the volumes of the plurality of arc-shaped touch pads are increasing in sequence. The inner wall of the curved surface is provided with a discharge slot on one side of the plurality of arc-shaped touch pads.

[0009] In a preferred embodiment, a plurality of reinforcing plates are installed at the bottom end of the side surface of the bracket, and the bracket is in a T-shape as a whole.

[0010] In a preferred embodiment, the joints between the guide surface, the groove surface and the curved surface are all arranged in a smooth transition.

[0011] In a preferred embodiment, the bending directions of the plurality of arcuate touch pads are toward the overall track direction of the curved surface, the track line formed by the overall outer sides of the plurality of arcuate touch pads is curved, and the concave direction of the curve points to one side of the guide surface.

[0012] In a preferred embodiment, the entire surfaces of the plurality of arc-shaped touch panels are all frosted, and the ends of the arc-shaped touch panels are semicircular.

[0013] In a preferred embodiment, the bottom end of the discharge notch and one side of the arc-shaped touch pad are located on the same horizontal plane, the discharge notch is in a strip-shaped structure as a whole, and the multiple discharge notches and multiple arc-shaped touch pads are all located within the trajectory range of the curved surface.

[0014] The technical effects and advantages of the present invention are as follows:

[0015] The present invention provides a toggle plate, a guide surface, a groove surface and a curved surface, and utilizes the mutual cooperation of the toggle plate, so that the tissue sample is driven to roll toward the groove surface and the curved surface under the action of flowing water, and the tissue sample is gradually brought into contact with the multiple arc-shaped touch plates on the surface of the curved surface. At this time, when the tissue sample gradually contacts the multiple arc-shaped touch plates from bottom to top, the rough surfaces of the arc-shaped touch plates can fully perform rolling friction with the tissue sample, so that the impurities adhering to the surface of the tissue sample are fully removed under the cleaning cooperation of the flowing water. In addition, the multiple arc-shaped touch plates with increasing volumes can also guide the tissue sample to move upward along the arc-shaped surface with increasing curvature, and after reaching the maximum height, the tissue sample rolls back to the side of the guide surface along the curved direction of the curved surface. The intermittent impact of the water flow can drive the toggle plate to rotate toward one side of the groove surface, so that the other side The curved surface directly contacts and collides with the tissue sample, promoting the tissue sample to accelerate and fall back into the inside of the groove surface, further enhancing its tumbling effect, and repeating this process, so that the tissue sample is always in a rapid tumbling state in a disordered trajectory, further promoting the cleaning effect of the tissue sample in sterile water, and in the process of continuous water supply, the water flow will be continuously discharged from the discharge slot under the action of its own fluidity, so that the cleaning water in the cleaning container is always in a renewed state, and the discharged water flow continuously discharges the cleaned adhesions until the entire sample is cleaned. During the entire cleaning process, the sample is fully exposed to the cleaning water and effectively friction-cleaned, while avoiding the generation of cleaning dead corners, effectively improving the degree of cleaning of impurities on the surface of the tissue sample, facilitating the subsequent detection procedures, and improving its working effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0017] Figure 2 It is a side structural schematic diagram of the present invention;

[0018] Figure 3 It is a schematic diagram of a half-section three-dimensional structure of the present invention;

[0019] Figure 4 It is a cross-sectional structural schematic diagram of the present invention;

[0020] Figure 5 A schematic diagram of the local distribution position of the arc-shaped touch panel of the present invention;

[0021] Figure 6 Schematic diagram of the distribution range of the guide surface, groove surface and curved surface of the present invention;

[0022] Figure 7 A schematic diagram of the rolling trajectory of the tissue sample and the motion trajectory of the paddle of the present invention;

[0023] Figure 8Schematic diagram of the outer track line distribution of multiple arc-shaped touch pads of the present invention.

[0024] The figures are marked as follows: 1. bracket; 2. cleaning container; 3. toggle plate; 4. rotating shaft; 5. arc-shaped touch plate; 6. discharge slot; 7. reinforcement plate; 31. raised surface; 32. arc-shaped surface; 21. guide surface; 22. groove surface; 23. curved surface. DETAILED DESCRIPTION

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] As attached Figure 1 To the attached Figure 8 The present invention relates to a pretreatment device for genetic testing, comprising a support 1, a cleaning container 2 mounted on the top of the support 1, a toggle plate 3 rotatably mounted on the inner wall of the cleaning container 2, a rotating shaft 4 fixedly mounted on the side of the toggle plate 3, and the rotating shaft 4 extending into the inner wall of the cleaning container 2 and rotatably fitting therewith.

[0027] The cross-sectional trajectory of the inner wall of the bottom end of the cleaning container 2 is provided with three sections of trajectories: a guide surface 21, a groove surface 22 and a curved surface 23, and the guide surface 21, the groove surface 22 and the curved surface 23 are distributed in sequence from left to right, and the guide surface 21 is a straight surface trajectory as a whole, and the overall trajectory of the groove surface 22 increases in sequence from left to right, and the overall curvature of the curved surface 23 is greater than the overall curvature of the groove surface 22, and the bending direction of the curved surface 23 is toward one side of the groove surface 22. Through the above arrangement, when the tissue specimen rolls to one side of the groove surface 22 and the curved surface 23 under the action of flowing water, it can move upward along the arc surface of the continuously increasing curvature of the two, and after reaching the maximum height, it rolls back to the side of the guide surface 21 along the bending direction of the curved surface 23, and rolls back and forth like this, thereby achieving sufficient cleaning and removing the surface The effect of removing surface debris is that among the guide surface 21, the groove surface 22 and the curved surface 23, the groove surface 22 has the lowest horizontal height, and the paddle plate 3 is located at an obliquely upper position of the groove surface 22. Through the above arrangement, the groove surface 22 has the lowest horizontal height, so that the tissue sample will always return to the inside of the groove surface 22 during the continuous rolling process, and roll over and over again, so that during the entire cleaning process, the sterile water always covers the entire tissue sample, and after rolling back to one side of the guide surface 21, the paddle plate 3 located at an obliquely upper position of the groove surface 22 can collide with the tissue sample, so that it is accelerated to fall back into the inside of the groove surface 22, further promoting its rolling effect, and increasing the disorder of its rolling trajectory, thereby improving its mixing effect inside the sterile water, and further improving the cleaning effect of the tissue sample.

[0028] The left and right sides of the bottom end of the toggle plate 3 are respectively provided with a convex surface 31 and a curved surface 32. One side of the bottom end of the convex surface 31 is in contact with the surface of the guide surface 21. The convex surface 31 and the curved surface 32 are transitioned by an arc surface. The overall curvature of the convex surface 31 is greater than the overall curvature of the curved surface 32. Through the above arrangement, the convex surface 31 on one side of the toggle plate 3 can fully contact the downward inclined water flow, and through the impact of the water flow, it is driven to rotate toward one side of the groove surface 22, so that the curved surface 32 on the other side directly contacts and collides with the tissue sample, causing it to accelerate back to the inside of the groove surface 22, further promoting its tumbling effect, and so on. The tissue sample is always in a rapid tumbling state in a disordered trajectory, further promoting the cleaning effect of the tissue sample in sterile water;

[0029] The inner wall of the curved surface 23 is equidistantly provided with a plurality of arc-shaped touch plates 5 from bottom to top, and the volumes of the plurality of arc-shaped touch plates 5 are in a trend of increasing in sequence. The inner wall of the curved surface 23 is provided with a discharge slot 6 on one side of the plurality of arc-shaped touch plates 5. The above arrangement ensures that when the tissue sample gradually contacts the plurality of arc-shaped touch plates 5 from bottom to top, the arc-shaped touch plates 5 fully roll and rub against the tissue sample, thereby removing impurities adhering to the surface of the tissue sample. In addition, the plurality of arc-shaped touch plates 5 with increasing volumes also guide the tissue sample toward one side of the guide surface 21, thereby achieving a reciprocating rolling cleaning effect.

[0030] A plurality of reinforcing plates 7 are installed at the bottom end of the side of the bracket 1. The bracket 1 is in a T-shape as a whole. The bending direction of the plurality of arc-shaped touch plates 5 is toward the overall trajectory direction of the curved surface 23. The trajectory line formed by the overall outer side of the plurality of arc-shaped touch plates 5 is in a curved state, and the concave direction of the curve points to one side of the guide surface 21. The overall surface of the plurality of arc-shaped touch plates 5 is frosted, and the end of the arc-shaped touch plate 5 is semicircular. Through the above arrangement, the friction effect between the arc-shaped touch plate 5 and the tissue sample can be ensured, and the rolling direction of the tissue sample can be guided and controlled, thereby ensuring the smoothness of the overall cleaning process and the cleaning effect.

[0031] For details, please refer to the attached manual Figure 4 The guide surface 21, the groove surface 22 and the curved surface 23 are all arranged in a smooth transition.

[0032] The specific implementation method is as follows: by utilizing the above-mentioned setting, it can be ensured that there are no protruding edges at the joints of the guide surface 21, the groove surface 22 and the curved surface 23, thereby increasing the smoothness of the tissue specimen and sterile water during the guided flow, and further improving the equipment's cleaning effect on the tissue specimen.

[0033] For details, please refer to the attached manual Figure 5 The bottom end of the discharge slot 6 is located on the same horizontal plane as one side of the arc-shaped touch pad 5 . The discharge slot 6 is in a strip-shaped structure as a whole. The multiple discharge slots 6 and the multiple arc-shaped touch pads 5 are all located within the trajectory range of the curved surface 23 .

[0034] The specific implementation method is as follows: using the above-mentioned arrangement, the arc-shaped touch plate 5 can continuously contact the surface of the tissue specimen when the tissue specimen is flushed by flowing water, thereby increasing the friction between the tissue specimen and other structures during the cleaning process, thereby achieving the purpose of sufficient cleaning.

[0035] Working principle of the present invention:

[0036] Step 1: First, the operator assembles the various components of the device normally, and places the cleaned tissue sample onto the groove surface 22 inside the cleaning container 2 through tweezers.

[0037] Step 2: First, the operator pours sterile water or pure water into the interior of the cleaning container 2, and allows it to gradually flow into the interior under the drainage of the guide surface 21. At this time, the flowing water gradually fills the interior of the groove surface 22, and under the action of the flowing water, drives the tissue sample to roll toward the groove surface 22 and the curved surface 23, and makes the tissue sample gradually contact the multiple arc-shaped touch plates 5 on the surface of the curved surface 23. At this time, when the tissue sample gradually contacts the multiple arc-shaped touch plates 5 from bottom to top, the rough surface of the arc-shaped touch plates 5 can be fully The tissue sample is subjected to rolling friction with the tissue sample, thereby fully removing the impurities adhering to the surface of the tissue sample under the cooperation of the running water cleaning, and the multiple arc-shaped touch plates 5 with increasing volumes will also guide the tissue sample to move upward along the arc surface with increasing curvature, and after reaching the maximum height, it will roll back to the side of the guide surface 21 along the bending direction of its curved surface 23. At the same time, the operator intermittently injects sterile water or pure water through a syringe or a flowing pouring method. At this time, the convex surface 31 on one side of the toggle plate 3 can fully contact the downwardly inclined surface. The water flow contacts the toggle plate 3 and the intermittent impact of the water flow, which drives it to rotate toward one side of the groove surface 22, so that the curved surface 32 on the other side directly contacts and collides with the tissue sample, promoting the tissue sample to accelerate back into the interior of the groove surface 22, further enhancing its tumbling effect. This reciprocating process makes the tissue sample always in a rapid tumbling state in a disordered trajectory, further promoting the cleaning effect of the tissue sample in sterile water. At the same time, during the intermittent water supply process, the toggle plate 3 itself will return to its original position due to the impact of the water flow. The frequency of the intermittent water supply between the toggle plate 3 and the operator is exactly the same as the frequency of the tissue sample tumbling and falling, thereby further improving the coordination of the equipment and enhancing the cleaning effect of the tissue sample. During the continuous water supply process, the water flow will continuously pass over the surface of the curved contact plate 5 under the action of its own fluidity and be continuously discharged from the discharge notch 6 on one side, so that the cleaning water in the cleaning container 2 is always in a renewed state, and the discharged water flow continuously discharges the washed adhesions until the sample is cleaned.

[0038] Step 3: First, the operator shuts down the device normally, then checks whether the fixation between the various components of the device is normal, and then replaces and repairs the aging and severely worn parts inside the device.

[0039] Finally, a few points should be explained: First, in the description of this application, it should be noted that, unless otherwise specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense, and may refer to mechanical or electrical connections, internal communication between two components, or direct connection. "Up," "down," "left," and "right" are only used to indicate relative positional relationships. When the absolute positions of the objects being described change, the relative positional relationships may also change.

[0040] Secondly: The drawings of the embodiments disclosed in the present invention only involve structures related to the embodiments disclosed in the present invention. Other structures may refer to conventional designs. The same embodiment and different embodiments of the present invention may be combined with each other without conflict.

[0041] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A pretreatment device for gene detection, characterized in that: The cleaning container is fixed on the top of the cleaning container, and a toggle plate is rotatably provided on the inner wall of the cleaning container. A rotating shaft is fixedly installed on the side of the toggle plate, and the rotating shaft extends into the inner wall of the cleaning container and is rotatably fitted with the inner wall of the cleaning container. The cross-sectional trajectory of the inner wall of the bottom end of the cleaning container is provided with three sections of trajectories: a guide surface, a groove surface and a curved surface. The guide surface, the groove surface and the curved surface are distributed in sequence from left to right. The guide surface is a straight surface trajectory as a whole. The overall trajectory of the groove surface increases in sequence from left to right. The overall curvature of the curved surface is greater than the overall curvature of the groove surface, and the bending direction of the curved surface is toward one side of the groove surface. Among the guide surface, the groove surface and the curved surface, the horizontal height of the groove surface is the lowest, and the toggle plate is located at a position obliquely above the groove surface. A convex surface and a curved surface are respectively provided on the left and right sides of the bottom end of the toggle plate. One side of the bottom end of the convex surface is in contact with the surface of the guide surface. A circular arc surface is provided as a transition between the convex surface and the curved surface. The overall curvature of the convex surface is greater than the overall curvature of the curved surface. The inner wall of the curved surface is equidistantly provided with a plurality of arc-shaped touch pads from bottom to top, and the volumes of the plurality of arc-shaped touch pads are increasing in sequence. The inner wall of the curved surface is provided with a discharge slot on one side of the plurality of arc-shaped touch pads.

2. A gene detection pretreatment device according to claim 1, characterized in that: A plurality of reinforcing plates are installed on the bottom end of the side surface of the bracket, and the bracket is in a T shape as a whole.

3. A gene detection pretreatment device according to claim 1, characterized in that: The joints between the guide surface, the groove surface and the curved surface are all arranged in a smooth transition.

4. A gene detection pretreatment device according to claim 1, characterized in that: The bending directions of the plurality of arc-shaped touch pads are toward the overall track direction of the curved surface. The track line formed by the overall outer sides of the plurality of arc-shaped touch pads is in a curved state, and the concave direction of the curve points to one side of the guide surface.

5. A gene detection pretreatment device according to claim 1, characterized in that: The entire surfaces of the plurality of arc-shaped touch panels are all frosted, and the ends of the arc-shaped touch panels are semicircular.

6. A gene detection pretreatment device according to claim 1, characterized in that: The bottom end of the discharge notch and one side of the arc-shaped touch pad are located on the same horizontal plane. The discharge notch is in a strip-shaped structure as a whole. The multiple discharge notches and the multiple arc-shaped touch pads are all located within the track range of the curved surface.

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