Device and method for measuring multiphase flow saturation and capillary pressure of dynamically cultured microorganisms

Abstract

The invention discloses a device and a method for measuring multiphase flow saturation and capillary pressure of dynamically cultured microorganisms, the device comprises an experiment box body, a nutrient solution conveying assembly, a non-wet phase box body and a glass tube, a porous plate and a porous stone which are sequentially distributed at intervals in the vertical direction are arranged in the upper end of the experiment box body; the experiment box body is provided with at least one liquid inlet, a first liquid outlet and a second liquid outlet, the liquid inlet is correspondingly arranged above the porous medium sample, the first liquid outlet is correspondingly arranged between the porous plate and the porous stone, and the second liquid outlet is correspondingly arranged below the porous stone; the nutrient solution conveying assembly comprises a nutrient solution tank, a first overflow tank and a second overflow tank, the first overflow tank is adjustable in height and connected with the nutrient solution tank and the solution inlet, and the second overflow tank is adjustable in height and connected with the first solution outlet; the non-wet phase box body is connected with the liquid inlet; the glass tube is connected with the second liquid outlet, is adjustable in height and is provided with scale marks; and establishing a dynamic change relationship between the capillary pressure and the saturation under different microbial biomass in the multiphase flow.

Description

Technical Field

[0001] The present invention relates to the technical field of fluid dynamics parameter measurement, and in particular to a device and method for measuring saturation and capillary pressure of multiphase flow of dynamically cultured microorganisms. Background Art

[0002] At present, groundwater polluted by the petroleum and chemical industries is mostly multiphase flow that is extremely difficult to treat. Saturation and capillary pressure are extremely important dynamic parameters in multiphase flow. The relationship between saturation and capillary pressure of multiphase flow in porous media is an important relationship in oil field development and groundwater pollution treatment.

[0003] The relationship between multiphase flow saturation and capillary pressure in porous media has been explored in many aspects at home and abroad, such as the type of porous media sample, the mathematical model used, the interfacial area between phases, etc., but most studies have ignored the impact of microorganisms on the relationship between the two. Summary of the Invention

[0004] The main purpose of the present invention is to provide a device and method for measuring the saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms, aiming to solve the above problems.

[0005] To achieve the above-mentioned object, the present invention proposes a device for measuring saturation and capillary pressure of a multiphase flow of dynamically cultured microorganisms, comprising:

[0006] An experimental box body is provided with a first opening at its upper end for injection of a microbial inoculum. A porous plate and a permeable stone are provided in the experimental box body. The porous plate and the permeable stone are sequentially spaced in the vertical direction. A porous medium sample is placed on the porous plate. The experimental box body is provided with at least one liquid inlet, a first liquid outlet, and a second liquid outlet. The liquid inlet is provided above the porous medium sample, the first liquid outlet is provided between the porous plate and the permeable stone, and the second liquid outlet is provided below the permeable stone.

[0007] A nutrient solution delivery assembly includes a nutrient solution tank, a first overflow tank, and a second overflow tank. The nutrient solution tank is used to accommodate nutrient solution. The first overflow tank is vertically adjustable so that it can be positioned higher than the porous medium sample. The first overflow tank is connected to the nutrient solution tank and the liquid inlet for inputting nutrient solution into the experimental chamber. The second overflow tank is vertically adjustable so that it can be positioned flush with the porous medium sample. The second overflow tank is connected to the first liquid outlet.

[0008] a non-wetting phase box containing a non-wetting phase fluid and connected to the liquid inlet for injecting the non-wetting phase fluid into the experimental box; and

[0009] A glass tube having a second opening at its upper end, the glass tube being connected to the second liquid outlet and having an adjustable height in the vertical direction, so as to generate negative pressure in the experimental box to drive the non-wetting phase fluid to penetrate into the porous medium sample, and to receive a mixed liquid of the microbial inoculum and nutrient solution flowing out of the porous medium sample and passing through the permeable stone. Scale lines are provided on the glass tube.

[0010] Optionally, a first baffle extending in the up-down direction is provided in the first overflow tank, wherein the bottom end of the first baffle abuts against the bottom wall of the first overflow tank, and a first gap is provided between the top end of the first baffle and the top end of the first overflow tank, so as to divide the first overflow tank into an infusion tank and a reflux tank that are connected;

[0011] The infusion tank is connected to the nutrient solution tank via a first water pipe and is connected to the liquid inlet via a second water pipe. The reflux tank is connected to the nutrient solution tank via a third water pipe.

[0012] Optionally, a peristaltic pump is provided on the first water pipe;

[0013] The second water pipe is provided with a first valve.

[0014] Optionally, the liquid inlet is provided with one, and the non-wetting phase box is connected to the second water pipe through a fourth water pipe.

[0015] Optionally, a second valve is provided on the fourth water pipe.

[0016] Optionally, a second baffle extending in the up-down direction is provided in the second overflow tank, the bottom end of the second baffle abuts against the bottom wall of the second overflow tank, and a second gap is provided between the top end of the second baffle and the top end of the second overflow tank, so as to divide the second overflow tank into a first recovery tank and a second recovery tank that are connected;

[0017] The first recovery tank is connected to the first liquid outlet through a fifth water pipe, and a third valve is provided on the fifth water pipe.

[0018] Optionally, a carrying plate is provided above the bottom end of the experimental box, the carrying plate is hollowed out, and the permeable stone is placed on the carrying plate;

[0019] A plurality of supporting columns are provided on the permeable stone for supporting the porous plate.

[0020] Optionally, the experimental box includes:

[0021] The box body is cylindrical and is arranged to penetrate in the up-down direction. The porous plate and the permeable stone are arranged in the box body. The first opening, the liquid inlet and the first liquid outlet are opened in the box body; and

[0022] The liquid collecting part is arranged at the lower end of the box body and is connected to the box body. The liquid collecting part is arranged in a funnel shape, and its diameter is gradually reduced in the up and down directions. The second liquid outlet is arranged at the bottom end of the liquid collecting part.

[0023] The present invention also provides a method for measuring the saturation and capillary pressure of a multiphase flow of a dynamic culture of microorganisms, which is applicable to a device for measuring the saturation and capillary pressure of a multiphase flow of a dynamic culture of microorganisms, and comprises the following steps:

[0024] Step S100: placing a porous medium sample on a porous plate, and injecting a microbial inoculum into the experimental box through a first opening of the experimental box;

[0025] Step S200: adjusting the height of the glass tube to drive the microbial inoculum to penetrate into the porous medium sample until the porous medium sample is saturated, and adjusting the zero scale line of the glass tube to be flush with the upper surface of the porous medium sample;

[0026] Step S300: adjusting the height of the first overflow tank so that the first overflow tank is located above the porous medium sample, and adjusting the height of the second overflow tank so that the second overflow tank is flush with the porous medium sample, and then continuously injecting nutrient solution into the experimental box through the liquid inlet of the experimental box according to the preset microorganism culture time;

[0027] Step S400: stop inputting the nutrient solution, and input a non-wetting phase fluid into the experimental box through the liquid inlet of the experimental box;

[0028] Step S500: Stop inputting the non-wetting phase fluid and move the glass tube downward, so that negative pressure is generated in the experimental chamber to drive the non-wetting phase fluid to penetrate into the porous medium sample and drive the microbial inoculum and nutrient solution in the porous medium sample to flow out, so that the microbial inoculum and nutrient solution pass through the permeable stone and flow into the glass tube through the second liquid outlet of the experimental chamber;

[0029] Step S600: When the liquid level change value of the liquid in the glass tube is within a preset range, the wetting phase fluid saturation, the non-wetting phase fluid saturation and the capillary pressure in the porous medium sample are measured, and a portion of the porous medium sample is removed from the experimental chamber to measure the microbial biomass therein;

[0030] Step S700: clean the experimental box, adjust the preset microorganism cultivation time, and repeat the above steps S100 to S600.

[0031] In the technical solution of the present invention, the influence of microbial factors on the relationship between saturation and capillary pressure is taken into account. By setting the nutrient solution tank, the first overflow tank and the second overflow tank to continuously input nutrient solution into the experimental box, continuous cultivation of microorganisms is achieved, and then by adjusting the height of the glass tube to generate negative pressure to drive the dynamic balance of multiphase flow in the experimental box, and measuring parameters in real time, continuous monitoring of the dynamic growth process of microorganisms is achieved, and then the dynamic change relationship between capillary pressure and saturation under different microbial biomass conditions in the multiphase flow is established, thereby capturing the influence of the continuous change of microbial biomass over time on the multiphase flow parameters, which is closer to the evolution process of microorganisms in the actual environment, that is, it conforms to the actual situation of multiphase flow management, and has broad applicability for the management of industrially polluted groundwater under natural conditions and the development of new bioremediation technologies. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0033] Figure 1 A schematic structural diagram of an embodiment of a device for measuring saturation and capillary pressure of a multiphase flow for dynamic microbial culture provided by the present invention;

[0034] Figure 2 A flow chart of the method for determining the relationship between saturation and capillary pressure of multiphase flow in porous media provided by the present invention;

[0035] Figure 3 This is a diagram showing the dynamic relationship between capillary pressure and saturation under different microbial biomass conditions in multiphase flow, established based on multiple measurement experimental results.

[0036] Description of Figure Numbers:

[0037]

[0038]

[0039] The purpose, features and advantages of the present invention will be further described with reference to the accompanying drawings and in conjunction with the embodiments. DETAILED DESCRIPTION

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0041] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the various components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indications will also change accordingly.

[0042] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or suggesting their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of such features. In addition, the meaning of "and / or" appearing throughout the text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme in which A and B are satisfied at the same time. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0043] At present, groundwater polluted by the petroleum and chemical industries is mostly multiphase flow that is extremely difficult to treat. Saturation and capillary pressure are extremely important dynamic parameters in multiphase flow. The relationship between saturation and capillary pressure of multiphase flow in porous media is an important relationship in oil field development and groundwater pollution treatment.

[0044] At present, the relationship between the saturation and capillary pressure of multiphase flow in porous media has been explored in many aspects at home and abroad, such as the type of porous media, the mathematical model used, the interfacial area between phases, etc., but most studies have ignored the impact of microorganisms on the relationship between the two.

[0045] In view of this, the present invention provides a device 100 for measuring saturation and capillary pressure of a multiphase flow of a dynamic culture microorganism. Figure 1 This is an embodiment of a device 100 for measuring saturation and capillary pressure of a multiphase flow of dynamically cultured microorganisms provided by the present invention.

[0046] See also Figure 1, the dynamic culture microbial multiphase flow saturation and capillary pressure measuring device 100 includes an experimental box 1, a nutrient solution delivery component 2, a non-wetting phase box 3 and a glass tube 4, the upper end of the experimental box 1 is provided with a first opening for injection of microbial inoculum, a porous plate 5 and a permeable stone 6 are provided in the experimental box 1, the porous plate 5 and the permeable stone 6 are spaced in sequence in the up and down directions, a porous medium sample 200 is placed on the porous plate 5, the experimental box 1 is provided with at least one liquid inlet, a first liquid outlet and a second liquid outlet, the liquid inlet is arranged above the porous medium sample 200, the first liquid outlet is arranged between the porous plate 5 and the permeable stone 6, and the second liquid outlet is arranged below the permeable stone 6; the nutrient solution delivery component 2 includes a nutrient solution tank 21, a first overflow tank 22 and a second overflow tank 23, the nutrient solution tank 21 is used to accommodate nutrient solution, the first overflow tank 22 is arranged in the up and down directions The height of the test box 1 is adjustable so that it can be set higher than the porous medium sample, and the first overflow tank 22 is connected to the nutrient solution tank 21 and the liquid inlet to input nutrient solution into the experimental box 1. The second overflow tank 23 is adjustable in height in the vertical direction so that it can be set flush with the porous medium sample 200, and the second overflow tank 23 is connected to the first liquid outlet; the non-wetting phase box 3 contains non-wetting phase fluid and is connected to the liquid inlet to inject the non-wetting phase fluid into the experimental box 1; the upper end of the glass tube 4 is provided with a second opening, and the glass tube 4 is connected to the second liquid outlet, and the height in the vertical direction is adjustable to generate negative pressure in the experimental box 1 to drive the non-wetting phase fluid to penetrate into the porous medium sample 200, and receive the microbial inoculum and nutrient solution flowing out of the porous medium sample 200 and passing through the permeable stone 6, and the glass tube 4 is provided with scale lines.

[0047] In the technical solution of the present invention, the influence of microbial factors on the relationship between saturation and capillary pressure is taken into consideration. By setting the nutrient solution tank 21, the first overflow tank 22 and the second overflow tank 23, nutrient solution is continuously input into the experimental box 1, that is, continuous cultivation of microorganisms is achieved. Then, by adjusting the height of the glass tube 4, negative pressure is generated to drive the dynamic balance of the multiphase flow in the experimental box 1, and parameters are measured in real time to achieve continuous monitoring of the dynamic growth process of microorganisms, and then a dynamic change relationship between capillary pressure and saturation under different microbial biomass conditions in the multiphase flow is established, so as to capture the influence of the continuous change of microbial biomass over time on the multiphase flow parameters, which is closer to the evolution process of microorganisms in the actual environment, that is, it conforms to the actual situation of multiphase flow management, and has broad applicability for the management of industrially polluted groundwater under natural conditions and the development of new bioremediation technologies.

[0048] It should be noted that, in the present invention, porous media sample 200 refers to a solid material containing a large number of pores, that is, a medium containing various types of capillary systems, such as pores and microcracks. Multiphase flow in porous media refers to a process in which porous media sample 200 serves as the solid phase, and the other phases are a mixed flow of nutrient solution, non-wetting phase fluid, and microbial inoculum, where the nutrient solution and microbial inoculum serve as the wetting phase fluids. More specifically, in one embodiment of the present invention, the non-wetting phase fluid is LNAPL (light non-aqueous phase liquid), i.e., an oil phase with a density less than that of water.

[0049] It should also be noted that, in the present invention, the permeable stone 6 only allows the liquid phase to pass through within its critical negative pressure.

[0050] It should also be noted that, in the present invention, the experimental box 1 is acid-resistant. More specifically, the experimental box 1 is made of acrylic plastic.

[0051] Further, see Figure 1 The first overflow tank 22 is provided with a first baffle 221 extending vertically. The bottom end of the first baffle 221 abuts against the bottom wall of the first overflow tank 22. A first gap is defined between the top of the first baffle 221 and the top of the first overflow tank 22, thereby dividing the first overflow tank 22 into an infusion trough 222 and a return trough 223. The infusion trough 222 is connected to the nutrient solution tank 21 via a first water pipe 24 and to the liquid inlet via a second water pipe 25. The return trough 223 is connected to the nutrient solution tank 21 via a third water pipe 26. Thus, the infusion trough 222 and the return trough 223 are connected via the first gap. When the liquid level of the nutrient solution injected from the nutrient solution tank 21 into the infusion trough 222 exceeds the height of the first baffle 221, the nutrient solution flows into the return trough 223, thereby maintaining a stable water head height, that is, ensuring a stable water pressure within the infusion trough 222.

[0052] It should be noted that the flow direction of the nutrient solution in the nutrient solution tank 21 is: the nutrient solution in the nutrient solution tank 21 first flows into the infusion tank 222 through the first water pipe 24. When the liquid level of the nutrient solution in the infusion tank 222 is lower than the height of the first baffle 221, all the nutrient solution in the infusion tank 222 flows into the experimental box 1 through the second water pipe 25 and the liquid inlet. When the liquid level of the nutrient solution in the infusion tank 222 is greater than the height of the first baffle 221, the nutrient solution above the first baffle 221 flows through the first gap to the reflux tank 223, and then flows back to the nutrient solution tank 21 through the third water pipe 26, and continues to provide nutrient solution to the infusion tank 222.

[0053] Further, see Figure 1 The first water pipe 24 is provided with a peristaltic pump 27 to continuously pump the nutrient solution in the nutrient solution tank 21 into the experimental box 1. The second water pipe 25 is provided with a first valve 28, which can be used to control the input of the nutrient solution, that is, to control whether the nutrient solution is input into the experimental box 1 and to control the input rate of the nutrient solution to meet the experimental requirements.

[0054] Specifically, in the present invention, the liquid inlet can be provided with two or one; when the liquid inlet is provided with two, the infusion tank 222 and the non-wetting phase box 3 are connected to the two liquid inlets in a one-to-one correspondence; and when the liquid inlet is provided with one, please refer to Figure 1 The non-wet phase chamber 3 is connected to the second water pipe 25 via a fourth water pipe 29. Thus, the non-wet phase fluid and the nutrient solution enter the experimental chamber 1 through the same liquid inlet, streamlining the structure. More specifically, the fourth water pipe 29 and the second water pipe 25 are connected via a three-way valve.

[0055] Further, see Figure 1 , a second valve 2a is provided on the fourth water pipe 29; in this way, the input of the non-wetting phase fluid can be controlled, that is, whether the non-wetting phase fluid is input into the experimental box 1 and the input rate of the non-wetting phase fluid can be controlled to meet the experimental requirements.

[0056] For details, please refer to Figure 1 The second overflow tank 23 is provided with a second baffle 231 extending vertically. The bottom end of the second baffle 231 abuts the bottom wall of the second overflow tank 23. A second gap is defined between the top of the second baffle 231 and the top of the second overflow tank 23, thereby dividing the second overflow tank 23 into a first recovery tank 232 and a second recovery tank 233. The first recovery tank 232 is connected to the first liquid outlet via a fifth water pipe 2b, which is provided with a third valve 2c. Thus, the first recovery tank 232 and the second recovery tank 233 are connected via the second gap. When the liquid level of the wet phase fluid (i.e., the microbial inoculum and nutrient solution) flowing into the first recovery tank 232 exceeds the height of the second baffle 231, it flows into the second recovery tank 233, thereby maintaining a stable water head height, that is, a stable water pressure in the first recovery tank 232. Furthermore, the third valve 2c controls the flow of the mixed liquid of the microbial inoculum and nutrient solution within the experimental chamber 1 to the first recovery tank 232.

[0057] It should be noted that the second recovery tank 233 is connected to an external water collecting tank.

[0058] For details, please refer to Figure 1 A carrying plate is provided above the bottom end of the experimental box 1 , the carrying plate is hollowed out, and the permeable stone 6 is placed on the carrying plate; a plurality of support columns 7 are provided on the permeable stone 6 to support the porous plate 5 .

[0059] For details, please refer to Figure 1 The experimental box 1 includes a box body and a liquid collecting part. The box body is cylindrical and is arranged to penetrate in the up and down directions. The porous plate 5 and the permeable stone 6 are arranged in the box body 11. The first opening, the liquid inlet and the first liquid outlet are opened in the box body 11; the liquid collecting part is arranged at the lower end of the box body and is connected to the box body. The liquid collecting part is funnel-shaped, and its diameter is gradually reduced in the up and down directions. The second liquid outlet is arranged at the bottom end of the liquid collecting part.

[0060] Specifically, in the present invention, the manner in which the first overflow tank 22, the second overflow tank 23, and the glass tube 4 are height-adjustable in the vertical direction is not limited. Specifically, in one embodiment of the present invention, the first overflow tank 22, the second overflow tank 23, and the glass tube 4 are each slidably mounted on a bracket and are movable in the vertical direction to achieve height adjustment. Of course, in another embodiment of the present invention, the first overflow tank 22, the second overflow tank 23, and the glass tube 4 are each detachably connected to one of a plurality of mounting portions on a bracket, such as by threaded connection, snap connection, etc., so that the height of the first overflow tank 22, the second overflow tank 23, and the glass tube 4 is adjustable.

[0061] The present invention also provides a method for measuring the saturation and capillary pressure of a dynamic culture microbial multiphase flow, which is applicable to the dynamic culture microbial multiphase flow saturation and capillary pressure measuring device described above. Figure 2 The method for determining the saturation and capillary pressure of a dynamic culture microbial multiphase flow comprises the following steps:

[0062] Step S100: placing a porous medium sample on a porous plate, and injecting a microbial inoculum into the experimental box through a first opening of the experimental box.

[0063] Step S200: Adjust the height of the glass tube to drive the microbial inoculum to penetrate into the porous medium sample until the porous medium sample is saturated, and adjust the 0 scale line of the glass tube to be flush with the upper surface of the porous medium sample.

[0064] In this step, after the height adjustment of the glass tube is completed, the measuring device is left to stand for 2 days.

[0065] Step S300: Adjust the height of the first overflow tank so that the first overflow tank is located above the porous medium sample, and adjust the height of the second overflow tank so that the second overflow tank is flush with the porous medium sample, and then continuously input nutrient solution into the experimental box through the liquid inlet of the experimental box according to the preset microbial culture time.

[0066] In this step, the heights of the first overflow tank and the second overflow tank are first adjusted, and then the peristaltic pump, the first valve and the third valve are opened to continuously inject nutrient solution into the experimental box to culture microorganisms.

[0067] Step S400: stop inputting the nutrient solution, and input non-wetting phase fluid into the experimental box through the liquid inlet of the experimental box.

[0068] In this step, the first valve is first closed to stop the input of the nutrient solution. After the liquid level in the experimental box stabilizes, the third valve is closed and the second valve is opened to input the non-wetting phase fluid into the experimental box.

[0069] Step S500: Stop inputting the non-wetting phase fluid and move the glass tube downward, so that negative pressure is generated in the experimental box to drive the non-wetting phase fluid to penetrate into the porous medium sample, and drive the microbial inoculum and nutrient solution in the porous medium sample to flow out, so that the microbial inoculum and nutrient solution pass through the permeable stone and then flow into the glass tube through the second liquid outlet of the experimental box.

[0070] In this step, the second valve is first closed to stop the input of the non-wetting phase fluid, and then the glass tube is moved downward. A negative pressure is generated in the experimental chamber, driving the non-wetting phase fluid on the upper side of the porous medium sample to penetrate into the porous medium sample, thereby displacing the microbial inoculum and nutrient solution, i.e., the wetting phase fluid, in the porous medium sample, so that the wetting phase fluid flows out of the porous medium sample and flows through the permeable stone into the glass tube through the second liquid outlet.

[0071] Step S600: When the liquid level change value of the liquid in the glass tube is within a preset range, the wetting phase fluid saturation, the non-wetting phase fluid saturation and the capillary pressure in the porous medium sample are measured, and a portion of the porous medium sample is removed from the experimental box to measure the amount of microorganisms therein.

[0072] In this step, the liquid level height, i.e., the liquid volume, in the glass tube is obtained by the scale lines on the glass tube, thereby obtaining the wetting phase fluid saturation, and then the non-wetting phase fluid saturation is calculated based on the sum of the wetting phase fluid saturation and the non-wetting phase fluid saturation being 1; at the same time, a ruler is used to measure the height difference between the liquid level in the glass tube and the top surface of the porous medium sample, thereby obtaining the capillary pressure, and then, based on the measurement results, a dynamic change relationship between the capillary pressure and saturation in multiphase flow is established.

[0073] In addition, in one embodiment of the present invention, a cation exchange resin method is used to extract EPS (extracellular polymers) to determine the quality of microorganisms. The cation exchange resin method is a prior art and will not be described in detail here.

[0074] It should be noted that when the liquid level change value in the glass tube is within the preset range, the liquid level in the glass tube tends to remain unchanged, that is, the saturation of the wetting phase fluid changes very little. At this time, the experiment is stopped and the saturation and capillary pressure are measured.

[0075] Step S700: clean the experimental box, adjust the preset microorganism cultivation time, and repeat the above steps S100 to S600.

[0076] In this step, the experimental box is cleaned, that is, the porous medium sample that has been tested is taken out and replaced with a new porous medium sample, and the preset microbial culture time is adjusted, that is, as the number of experiments increases, the preset microbial culture time is also extended, so that the amount of microorganisms in each experiment is different. More specifically, the extension interval is 1 day, that is, in the first experiment, the preset microbial culture time is 1 day, in the second experiment, the preset microbial culture time is adjusted to 2 days, in the third experiment, the preset microbial culture time is adjusted to 3 days, and so on. Furthermore, based on the results of multiple measurements of the experiment, a dynamic change relationship between capillary pressure and saturation under different microbial amounts in multiphase flow is established (such as Figure 3 shown).

[0077] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention description and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms, characterized in that: The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms comprises: An experimental box body is provided with a first opening at its upper end for injection of a microbial inoculum. A porous plate and a permeable stone are provided in the experimental box body. The porous plate and the permeable stone are sequentially spaced in the vertical direction. A porous medium sample is placed on the porous plate. The experimental box body is provided with at least one liquid inlet, a first liquid outlet, and a second liquid outlet. The liquid inlet is provided above the porous medium sample, the first liquid outlet is provided between the porous plate and the permeable stone, and the second liquid outlet is provided below the permeable stone. A nutrient solution delivery assembly includes a nutrient solution tank, a first overflow tank, and a second overflow tank. The nutrient solution tank is used to accommodate nutrient solution. The first overflow tank is vertically adjustable so that it can be positioned higher than the porous medium sample. The first overflow tank is connected to the nutrient solution tank and the liquid inlet for inputting nutrient solution into the experimental chamber. The second overflow tank is vertically adjustable so that it can be positioned flush with the porous medium sample. The second overflow tank is connected to the first liquid outlet. a non-wetting phase box containing a non-wetting phase fluid and connected to the liquid inlet for injecting the non-wetting phase fluid into the experimental box; and A glass tube having a second opening at its upper end, the glass tube being connected to the second liquid outlet and having an adjustable height in the vertical direction, so as to generate negative pressure in the experimental box to drive the non-wetting phase fluid to penetrate into the porous medium sample, and to receive the microbial inoculum and nutrient solution flowing out of the porous medium sample and passing through the permeable stone, wherein the glass tube is provided with scale lines.

2. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 1, characterized in that: A first baffle extending in the up-down direction is provided in the first overflow tank, wherein the bottom end of the first baffle abuts against the bottom wall of the first overflow tank, and a first gap is provided between the top end of the first baffle and the top end of the first overflow tank to divide the first overflow tank into an infusion tank and a reflux tank that are connected; The infusion tank is connected to the nutrient solution tank via a first water pipe and is connected to the liquid inlet via a second water pipe. The reflux tank is connected to the nutrient solution tank via a third water pipe.

3. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 2, characterized in that: The first water pipe is provided with a peristaltic pump; The second water pipe is provided with a first valve.

4. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 2, wherein: The liquid inlet is provided with one, and the non-wetting phase box is connected to the second water pipe through a fourth water pipe.

5. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 4, characterized in that: The fourth water pipe is provided with a second valve.

6. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 1, characterized in that: A second baffle is provided in the second overflow tank and extends in the up-down direction. The bottom end of the second baffle abuts against the bottom wall of the second overflow tank. A second gap is provided between the top end of the second baffle and the top end of the second overflow tank to divide the second overflow tank into a first recovery tank and a second recovery tank that are connected. The first recovery tank is connected to the first liquid outlet through a fifth water pipe, and a third valve is provided on the fifth water pipe.

7. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 1, characterized in that: A carrying plate is provided above the bottom end of the experimental box, the carrying plate is hollowed out, and the permeable stone is placed on the carrying plate; A plurality of supporting columns are provided on the permeable stone for supporting the porous plate.

8. The device for measuring saturation and capillary pressure of multiphase flow of dynamic cultured microorganisms according to claim 1, wherein: The experimental box comprises: The box body is cylindrical and is arranged to penetrate in the up-down direction. The porous plate and the permeable stone are arranged in the box body. The first opening, the liquid inlet and the first liquid outlet are opened in the box body; and The liquid collecting part is arranged at the lower end of the box body and is connected to the box body. The liquid collecting part is arranged in a funnel shape, and its diameter is gradually reduced in the up and down directions. The second liquid outlet is arranged at the bottom end of the liquid collecting part.

9. A method for measuring saturation and capillary pressure of a multiphase flow of a dynamic culture of microorganisms, applicable to the device for measuring saturation and capillary pressure of a multiphase flow of a dynamic culture of microorganisms as claimed in any one of claims 1 to 8, characterized in that: The method for measuring saturation and capillary pressure of a dynamic culture microbial multiphase flow comprises the following steps: Step S100: placing a porous medium sample on a porous plate, and injecting a microbial inoculum into the experimental box through a first opening of the experimental box; Step S200: adjusting the height of the glass tube to drive the microbial inoculum to penetrate into the porous medium sample until the porous medium sample is saturated, and adjusting the zero scale line of the glass tube to be flush with the upper surface of the porous medium sample; Step S300: adjusting the height of the first overflow tank so that the first overflow tank is located above the porous medium sample, and adjusting the height of the second overflow tank so that the second overflow tank is flush with the porous medium sample, and then continuously injecting nutrient solution into the experimental box through the liquid inlet of the experimental box according to the preset microorganism culture time; Step S400: stop inputting the nutrient solution, and input a non-wetting phase fluid into the experimental box through the liquid inlet of the experimental box; Step S500: Stop inputting the non-wetting phase fluid and move the glass tube downward, so that negative pressure is generated in the experimental chamber to drive the non-wetting phase fluid to penetrate into the porous medium sample and drive the microbial inoculum and nutrient solution in the porous medium sample to flow out, so that the microbial inoculum and nutrient solution pass through the permeable stone and flow into the glass tube through the second liquid outlet of the experimental chamber; Step S600: When the liquid level change value of the liquid in the glass tube is within a preset range, the wetting phase fluid saturation, the non-wetting phase fluid saturation and the capillary pressure in the porous medium sample are measured, and a portion of the porous medium sample is removed from the experimental chamber to measure the microbial biomass therein; Step S700: clean the experimental box, adjust the preset microorganism cultivation time, and repeat the above steps S100 to S600.

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