Hippocampal peptide hand cream and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and particularly discloses hippocampal peptide hand cream and a preparation method thereof. The hand cream takes hippocampal peptide as a core active component, and further comprises span 80, Tween 80, stearic acid, hexadecanol, glycerol, jojoba oil, rose essential oil and the like. The prepared hippocampal peptide hand cream product is good in moisturizing performance; after a human body trials for 4 weeks, the moisture value, the elasticity value and the whiteness of the skin are remarkably improved, layering and discoloration are avoided through centrifugation, cold resistance, heat resistance and illumination tests, and the safety meets the requirements of cosmetic safety technical specifications.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics and preparation methods thereof, and in particular to a hippocampal peptide hand cream and a preparation method thereof. Background Art

[0002] Long-term exposure to the external environment, frequent contact with detergents, UV radiation, and temperature and humidity fluctuations can lead to moisture loss in the stratum corneum and impaired barrier function, leading to dryness, roughness, and even cracking and aging. As a basic hand care product, hand creams must possess multiple benefits. However, traditional formulas, with glycerin, petrolatum, or plant oils as core ingredients, rely primarily on a water-locking film for basic moisturization, failing to meet the needs of deeper repair and anti-aging.

[0003] As a precious traditional Chinese medicine, hippocampus (Hippocampus) contains active peptides with excellent antioxidant, anti-inflammatory, tissue repair, and whitening properties (Chen L, Wang X, Huang B. The genus Hippocampus - A review on traditional medicinal uses, chemical constituents and pharmacological Properties [J]. Journal of Ethnopharmacology, 2015, 162: 104-111.). They are highly effective in scavenging free radicals and inhibiting collagen degradation. Small-molecule peptides are also more easily absorbed through the skin, thereby increasing the water-holding capacity of the stratum corneum. Currently, the application of hippocampus peptides in hand creams is still unexplored.

[0004] Based on single-factor experimental screening technology, the present invention constructs a hand cream formula with hippocampal peptide as the core active ingredient, obtains a product with better properties by optimizing the preparation process, and adopts a multi-dimensional evaluation system to verify the product performance, thus filling the technical gap in this field. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a hippocampal peptide hand cream and a preparation method thereof in view of the deficiencies in the prior art.

[0006] In order to achieve the purpose of the present invention, the following technical means are specifically adopted:

[0007] A hippocampal peptide hand cream, characterized in that it comprises the following components in percentage by mass, and the sum of the contents of each component is 100%:

[0008] Hippocampal peptide: 2.5-17.5%, of which protein content (on dry basis) ≥80%, peptide content (on dry basis) ≥98%, and the proportion of protein hydrolysates with a relative molecular mass of peptides less than 500 ≥95%;

[0009] Emulsifying system: Span 80, Tween 80, glyceryl stearate, stearic acid and cetyl alcohol, wherein the mass ratio of Span 80 to Tween 80 is 1: (1-3.5), and the mass ratio of stearic acid to cetyl alcohol is 1: (0.5-1.5);

[0010] Moisturizer: glycerin 8-12%;

[0011] Emollient: jojoba oil 4-6%, lanolin and petrolatum mass ratio (0.5-3):1;

[0012] Fragrance: rose essential oil 0.1-0.3%;

[0013] Preservative: sodium benzoate 0.2%;

[0014] Solvent: deionized water;

[0015] The preparation method of the hand cream comprises: (a) melting and dispersing Span 80, stearic acid, glyceryl stearate, lanolin, cetyl alcohol, jojoba oil, and vaseline at 70°C to prepare component A; (b) stirring and dissolving Tween 80, glycerol, and deionized water at 70°C to prepare component B; (c) slowly pouring component A into component B, stirring and emulsifying at 70-80°C and 800-1200 r / min for 3-11 minutes; and (d) cooling the mixture to 40°C, adding hippocampal peptide, rose essential oil, and sodium benzoate, and stirring and cooling the mixture to room temperature.

[0016] The hippocampus peptide hand cream is characterized in that the mass ratio of Span 80 to Tween 80 in the emulsification system is 2.5:2.5, and the mass ratio of stearic acid to hexadecanol is 1:1.5.

[0017] The hippocampus peptide hand cream is characterized in that the amount of glycerin is 8%, the amount of jojoba oil is 4%, and the amount of rose essential oil is 0.2%.

[0018] The preparation method of the hippocampus peptide hand cream is characterized in that the process parameters of step (c) are: emulsification temperature 75° C., stirring speed 1000 r / min, and emulsification time 7 minutes.

[0019] The hippocampus peptide hand cream is used for non-therapeutic improvement of moisture, elasticity and whiteness of hand skin.

[0020] The hippocampal peptide described in the present invention is powdery, free of lumps, white to light yellow, free of external visible impurities, with a protein content (on a dry basis) of ≥80%, a peptide content (on a dry basis) of ≥98%, a proportion of protein hydrolysates with a peptide relative molecular mass of less than 500 of ≥95%, a moisture content of ≤7g / 100g, and an ash content of ≤8g / 100g.

[0021] Beneficial effects

[0022] Beneficial effects of the present invention:

[0023] The hippocampus peptide hand cream presented by the present invention has stable quality, good smell, excellent moisturizing performance, can significantly improve the moisture, elasticity and whiteness of the hand skin, and has good safety. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0025] Description of the hippocampal peptide used in the present invention: the manufacturer is Xi'an Danais Biotechnology Co., Ltd., the product batch number is DNS20240609, the protein content (on a dry basis) is 88.06%, the peptide content (on a dry basis) is 99.50%, the proportion of protein hydrolysates with a relative molecular mass of peptides less than 500 is 95.1%, the water content is 3.62 g / 100 g, and the ash content is 4.33 g / 100 g.

[0026] Example 1 Hand Cream Composition Screening

[0027] 1. Preliminary formulation of prescription

[0028] Based on the general composition of O / W hand cream and the physicochemical properties of hippocampal peptide, the preliminary formula is shown in Table 1.

[0029] Table 1 Preliminary prescription

[0030]

[0031] 2. Preliminary preparation method

[0032] (1) Place Span 80, stearic acid, glyceryl stearate, lanolin, cetyl alcohol, jojoba oil, and vaseline in a beaker and stir at 70°C until dispersed, as component A;

[0033] (2) Weigh Tween 80, glycerol, and water into a beaker and stir at 70°C until completely dissolved. This is component B.

[0034] (3) Slowly pour component A into component B and emulsify evenly for a period of time. The emulsification temperature is 70°C, the emulsification time is 7 minutes, and the stirring rate is 1000 r / min. Slowly stir and cool to 40°C, then add component C and continue stirring and cooling to room temperature.

[0035] 3. The calculation and measurement methods of some evaluation indicators are as follows

[0036] 3.1 Sensory evaluation and stability determination

[0037] Ten volunteers aged 18 to 60 with healthy skin were randomly selected to undergo a sensory evaluation of the hand creams under the same conditions. The specific evaluation criteria are shown in Table 2 below.

[0038] Table 2 Hand cream sensory index scoring standard

[0039]

[0040]

[0041] 3.2 Aroma intensity determination

[0042] Ten volunteers aged 18 to 60 with healthy skin were randomly selected to try the hand cream. Their aroma was rated and the average value was calculated. The specific evaluation criteria are shown in Table 3 below.

[0043] Table 3 Scoring criteria for hand cream fragrance intensity

[0044] score Strength Description Acceptance Description 1 Almost fragrance-free, only detectable when applied close to the skin Low acceptance ("lack of fragrance") 2 Light, faintly odorous after application Acceptable, but some users would like it to be more obvious 3 Moderate, clearly discernible and not pungent after application Optimal acceptance interval ("pleasing") 4 Rich, but not reaching the discomfort threshold Some users think it is too concentrated 5 Pungent or chemical smell General rejection ("cheap / pungent scent")

[0045] 4. Single factor screening of prescription composition

[0046] 4.1 Investigation of glycerol dosage

[0047] Excessive amounts of glycerin in a formulation can cause moisture absorption and stickiness, while insufficient amounts can lead to insufficient moisturizing effects. Formulas with 8%, 10%, and 12% glycerin were investigated, and the water content was adjusted to maintain a 100% glycerin content. Samples were prepared using the proposed method, and the glycerin content was selected based on moisturizing performance measurements. The experimental results are shown in Table 4 below.

[0048] Table 4 Single factor investigation results of glycerol dosage

[0049]

[0050] The results in the table above show that when the glycerol dosage ranged from 8% to 12%, the overall prescription score was good (>7 points), indicating that prescriptions with a glycerol dosage of 8% to 12% were all qualified. It was also noted that the 8% glycerol dosage had the highest score, so this dosage was temporarily selected for subsequent screening studies.

[0051] 4.2 Investigation of the compound ratio of stearic acid and hexadecanol

[0052] The combination of stearic acid and cetyl alcohol can affect the texture, stability, and moisturizing properties of a paste. Using an 8% glycerin formulation, three ratios of stearic acid:cetyl alcohol were investigated: 1:0.5 (2%:1%), 1:1 (2%:2%), and 1:1.5 (2%:3%). The water content was adjusted to maintain a 100% water content. Samples were prepared using the proposed method, and the sensory scores of the samples prepared with different ratios were compared. The experimental results are shown in Table 5 below.

[0053] Table 5 Investigation results of compounding ratio of stearic acid and hexadecanol

[0054]

[0055] The results in the table above show that the overall scores for the formulations were good (>7 points) when the stearic acid:hexadecanol ratios were 1:0.5 (2%:1%), 1:1 (2%:2%), and 1:1.5 (2%:3%), indicating that the formulations were qualified within the 1:1 ratio (0.5-1.5). It was also noted that the highest score was achieved when the stearic acid:hexadecanol ratio was 1:1.5, so a 1:1.5 ratio was temporarily selected for subsequent screening studies.

[0056] 4.3 Investigation of the compound ratio of Span 80 and Tween 80

[0057] By adjusting the ratio of the two, the HLB value of the mixture was brought close to the HLB required by the oil phase, thus forming a stable O / W emulsified system. The formulation used 8% glycerol, and a 1:1.5 (2%:3%) ratio of stearic acid to cetyl alcohol. Three ratios of Span 80:Tween 80 (1:3.5%), 2:3 (2%:3%), and 2.5:2.5 (2.5%:2.5%) were investigated. The water content was adjusted to maintain a 100% HLB ratio throughout the formulation. Samples were prepared using the proposed method, and the sensory scores of the samples prepared with different ratios were compared. The experimental results are shown in Table 6 below.

[0058] Table 6 Results of the compounding ratio of Span 80 and Tween 80

[0059] Ratio Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 1：3.5 6 8 6 7 8 8 7.2 2：3 8 9 8 8 8 8 8.2 2.5：2.5 7 9 9 9 8 9 8.5

[0060] The results in the table above show that the overall scores for the prescriptions were good (>7 points) when the Span 80:Tween 80 ratios were 1:3.5 (1%:3.5%), 2:3 (2%:3%), and 2.5:2.5 (2.5%:2.5%), indicating that the Span 80:Tween 80 ratio within the 1:1 to 3.5 range was acceptable. It was also noted that a Span 80:Tween 80 ratio of 2.5:2.5 achieved the highest score, so a Span 80:Tween 80 ratio of 2.5:2.5 was temporarily selected for subsequent screening studies.

[0061] 4.4 Study on the dosage of jojoba oil prescription

[0062] The amount of glycerol in the prescription was selected to be 8%, the ratio of stearic acid to cetyl alcohol was 1:1.5 (2%:3%), the ratio of Span 80 to Tween 80 was 2.5:2.5 (2.5%:2.5%), and three ratios of jojoba oil dosage of 4%, 5% and 6% were investigated respectively. The amount of water was adjusted to keep the prescription at 100%. The samples were prepared using the proposed preparation method, and the sensory scores of the samples prepared with different ratios were compared. The experimental results are shown in Table 7 below.

[0063] Table 7 Results of the investigation on the dosage of jojoba oil prescription

[0064] Prescription amount (%) Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 4 9 8 9 9 8 8 8.5 5 9 8 7 8 8 9 8.2 6 9 9 6 8 9 9 8.3

[0065] The results in the table above show that the overall scores for jojoba oil prescriptions at 4%, 5%, and 6% were all good (>7 points), indicating that prescriptions with jojoba oil dosages ranging from 4% to 6% were all qualified. It was also noted that the 4% jojoba oil dosage had the highest score, so the 4% jojoba oil dosage was temporarily selected for subsequent screening studies.

[0066] 4.5 Study on the ratio of lanolin and vaseline

[0067] Lanolin and petrolatum require an appropriate ratio and dosage to balance the sealing and breathability of the final product. The formulation used 8% glycerin, a 1:1.5 ratio of stearic acid to cetyl alcohol (2%:3%), a 2.5:2.5 ratio of Span 80 to Tween 80 (2.5%:2.5%), and 4% jojoba oil. Three lanolin:petrolatum ratios of 1:0.5 (1%:0.5%), 1.5:0.5 (2%:1%), and 0.5:1 (0.5%:1%) were investigated. The water content was adjusted to maintain a constant 100% water content. Samples were prepared using the proposed method, and the sensory scores of the samples prepared with different ratios were compared. The experimental results are shown in Table 8 below.

[0068] Table 8 Lanolin and Vaseline Ratio Investigation Results

[0069] Ratio Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 1：0.5 9 9 9 8 8 8 8.5 1.5：0.5 9 9 7 8 8 9 8.3 0.5：1 9 9 7 7 8 8 8

[0070] The results in the table above show that the overall scores for the formulas were good (>7 points) for lanolin: petrolatum ratios of 1:0.5 (1%:0.5%), 1.5:0.5 (2%:1%), and 0.5:1 (0.5%:1%), indicating that formulas with a lanolin: petrolatum ratio of 0.5-3:1 were all acceptable. It was also noted that a lanolin: petrolatum ratio of 1:0.5 (1%:0.5%) scored the highest, so a lanolin: petrolatum ratio of 1:0.5 was temporarily selected for subsequent screening studies.

[0071] 4.6 Investigation of hippocampal peptide dosage

[0072] The amount of glycerol in the prescription was selected to be 8%, the ratio of stearic acid to hexadecanol was 1:1.5 (2%:3%), the ratio of Span 80 to Tween 80 was 2.5:2.5 (2.5%:2.5%), the ratio of jojoba oil was 4%, and the ratio of lanolin to vaseline was 1:0.5 (1%:0.5%). The amounts of hippocampal peptide in the prescriptions were investigated at 0%, 2.5%, 10% and 17.5%, respectively. The amount of water was adjusted to keep the prescription at 100%. The samples were prepared using the proposed preparation method, and the sensory scores of the samples prepared with different ratios were compared. The experimental results are shown in Table 9 below.

[0073] Table 9 Results of the investigation on the dosage of hippocampal peptide prescription

[0074] Prescription amount (%) Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 0 8 8 8 5 6 6 6.8 2.5 9 9 9 8 8 8 8.5 10 9 9 9 7 8 8 8.3 17.5 9 8.5 9 7 7 8 8.1

[0075] As can be seen from the results in the table above, when the dosage of hippocampal peptide in the prescription is 2.5%, 10% and 17.5%, the overall score of the prescription is good (>7 points), indicating that the dosage ratio of hippocampal peptide in the prescription is qualified within the range of 2.5-17.5%. It is also noted that the highest score is obtained when the dosage ratio of hippocampal peptide is 2.5%, so the dosage ratio of hippocampal peptide is temporarily selected for subsequent screening studies.

[0076] 4.7 Investigation of Rose Essential Oil Dosage

[0077] The formulation used 8% glycerol, a 1:1.5 ratio of stearic acid to hexadecanol (2%:3%), a 2.5:2.5 ratio of Span 80 to Tween 80 (2.5%:2.5%), a 4% ratio of jojoba oil, a 1:0.5 ratio of lanolin to petrolatum (1%:0.5%), and a 2.5% ratio of hippocampal peptide. Rose essential oil was tested at 0.1%, 0.2%, and 0.3% of the formulation, respectively. The water content was adjusted to maintain a 100% formulation. Samples were prepared using the proposed preparation method. The optimal fragrance ratio was selected by evaluating aroma intensity. The experimental results are shown in Table 10.

[0078] Table 10 Results of investigation on flavor dosage

[0079] Fragrance dosage Average aroma intensity rating 0.1％ 2.6 0.2％ 3 0.3％ 3.8

[0080] The results in the table above show that the overall aroma intensity scores for the prescriptions at 0.1%, 0.2%, and 0.3% rose essential oil were all within the acceptable range (2 to 4 points), indicating that the 0.1-0.3% rose essential oil dosage range was acceptable. It was also noted that the aroma intensity score was optimal at 0.2%, so 0.2% rose essential oil was temporarily selected for subsequent screening studies.

[0081] According to the above-mentioned results of the prescription composition investigation, the prescription composition is determined as shown in Table 11 below.

[0082] Table 11 Prescription composition

[0083]

[0084] Note: The qualified range of glycerin dosage is 8-12%; the qualified range of the ratio of stearic acid to cetyl alcohol is 1:(0.5-1.5); the qualified range of the ratio of Span 80 to Tween 80 is 1:(1-3.5); the qualified range of the ratio of jojoba oil is 4-6%; the qualified range of the ratio of lanolin to vaseline is (0.5-3):1; the qualified range of hippocampal peptide dosage is 2.5-17.5%; the qualified range of rose essential oil dosage is 0.1-0.3%.

[0085] Example 2 Screening of the Preparation Process for Hippocampal Peptide Hand Cream

[0086] In each investigation under this embodiment, the prescription composition in Table 11 was used.

[0087] 1. Investigation of feeding method

[0088] Method (1) Water-to-oil phase method: Place component A (oil phase) in a beaker and stir at a constant temperature of 70°C until dispersed. Place component B (water phase) in a beaker and stir at a constant temperature of 70°C until dispersed. Slowly pour component A into component B, emulsification temperature 70°C, emulsification time 7 minutes, stirring rate 1000r / min, slowly stir and cool to 40°C, add component C, and continue stirring and cooling to room temperature.

[0089] Method (2) Mixing and heating method: Place component A (oil phase) in a beaker and stir at a constant temperature of 70°C until dispersed. Place component B (water phase) in a beaker and stir at a constant temperature of 70°C until dispersed. Quickly pour component A into component B, emulsify at 70°C, emulsify for 7 minutes, stir at a rate of 1000 r / min, slowly stir and cool to 40°C, add component C and continue stirring to cool to room temperature.

[0090] The samples prepared by the above two methods were subjected to sensory evaluation, and the method with the higher score was used as the feeding method, and subsequent experiments were carried out. The experimental results are shown in Table 12 below.

[0091] Table 12 Results of investigation on feeding method

[0092] Feeding method Color Graininess greasy feeling Smoothness Hydration Moisturizing average value Water-phase oil-phase method 9 9 9 8 8 8 8.5 Mixed heating method 7 6 6 6 7 8 6.7

[0093] As can be seen from the results in the table above, the overall score of the samples prepared by the method (1) of adding oil to the aqueous phase is significantly better than that of the samples prepared by the method (2) of mixing and heating. Therefore, the preparation method of the hippocampus peptide hand cream adopts the method (1) of adding oil to the aqueous phase.

[0094] 2. Emulsification time inspection

[0095] The oil phase was added to the water phase method, and the emulsification time was set to 3 min, 7 min and 11 min respectively. The sensory scores of samples with different emulsification times were compared. The results are shown in Table 13 below.

[0096] Table 13 Emulsification time investigation results

[0097] Emulsification time (min) Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 3 8 6 6 7 8 7 7 7 10 8 8 9 8 9 8.7 11 7 9 7 7 8 9 7.8

[0098] The results in the table above show that the overall scores for samples prepared with emulsification times of 3, 7, and 11 minutes were all good (≥7 points), indicating that emulsification times within the 3-11 minute range are all acceptable. It was also noted that the highest score was achieved with an emulsification time of 7 minutes, so this emulsification time was temporarily selected for subsequent screening studies.

[0099] 3. Emulsification temperature investigation

[0100] The oil phase addition method was adopted, the emulsification time was set to 7 min, and the emulsification temperatures were set to 70°C, 75°C and 80°C respectively. The sensory scores of the samples prepared at different emulsification temperatures were compared. The results are shown in Table 14 below.

[0101] Table 14 Emulsification temperature investigation results

[0102] Emulsification temperature (℃) Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 70 10 8 8 9 8 9 8.7 75 10 8 9 9 8 9 8.8 80 9 9 7 9 8 9 8.5

[0103] The results in the table above show that the overall scores of samples prepared at emulsification temperatures of 70°C, 75°C, and 80°C were all good (≥7 points), indicating that the emulsification temperature range of 70-80°C is acceptable. It was also noted that the emulsification temperature of 75°C achieved the highest score, so 75°C was temporarily selected for subsequent screening studies.

[0104] 4. Investigation of stirring speed

[0105] The oil phase addition method was adopted, the emulsification time was set to 7 min, the emulsification temperature was 75°C, and the stirring speeds were set to 800 r / min, 1000 r / min and 1200 r / min respectively. The sensory scores of the samples prepared at different stirring speeds were compared. The results are shown in Table 15 below.

[0106] Table 15 Stirring speed investigation results

[0107] Stirring speed (r / min) Color Graininess greasy feeling Smoothness Hydration Moisturizing average value 800 9 7 6 7 8 7 7.3 1000 9 8 9 8 8 9 8.5 1200 9 7 7 9 8 9 8.2

[0108] The results in the table above show that the overall scores of the samples prepared at stirring speeds of 800 r / min, 1000 r / min, and 1200 r / min were all good (≥7 points), indicating that stirring speeds in the range of 800 to 1200 r / min were all qualified. It is also noted that the highest score was achieved at a stirring speed of 1000 r / min.

[0109] Based on the above investigation results, the preparation method of this product is as follows:

[0110] (1) Place Span 80, stearic acid, glyceryl stearate, lanolin, cetyl alcohol, jojoba oil, and vaseline in a beaker and stir at 70°C until dispersed, as component A;

[0111] (2) Weigh Tween 80, glycerol, and water into a beaker and stir at 70°C until completely dissolved. This is component B.

[0112] (3) Slowly pour component A into component B and emulsify evenly for a period of time. The emulsification temperature is 70-80°C, the emulsification time is 3-11 minutes, and the stirring rate is 800-1200 r / min. Slowly stir and cool to 40°C, then add component C and continue stirring and cooling to room temperature.

[0113] Example 3 Performance Evaluation of Hippocampal Peptide Hand Cream

[0114] In each investigation under this embodiment, the hippocampal peptide hand cream samples were prepared using the prescription composition in Table 11 and the preparation method optimized in Example 2.

[0115] 1.Physical and chemical properties

[0116] 1.1 Appearance and texture

[0117] White emulsion paste, fine and uniform texture, no granularity, light rose scent.

[0118] 1.2 pH value

[0119] Weigh 3g of hand cream (accurate to 0.1g) and add nine parts of boiled and cooled test water. Heat to 40°C, stirring continuously, and cool to room temperature before use. Before measurement, calibrate with two standard buffers. After calibration, rinse the electrode with water, then blot dry with filter paper. Carefully insert the electrode into the sample, immersing it. Wait for the pH meter reading to stabilize and record the reading.

[0120] Measurement results: 5.76, 5.74, 5.69, 5.92, 5.83, 5.89, 5.74, 5.76, 5.84.

[0121] 1.3 Stability Test

[0122] (1) Centrifugation test: 1.5 g of sample was transferred into a 2 mL EP tube and centrifuged at 3000 r / min for 30 min. The hand cream was observed for stratification.

[0123] Result: No stratification or oil-water separation.

[0124] (2) Cold resistance test:

[0125] Set the refrigerator temperature to -8°C, transfer 1.5 g of the sample into a 2 mL EP tube, and place it in the refrigerator. Take it out after 24 hours and observe whether the hand cream has stratification.

[0126] Results: The paste remained homogeneous after returning to room temperature.

[0127] (3) Heat resistance test:

[0128] Set the constant temperature incubator to 40°C, transfer 1.5 g of the sample into a 2 mL EP tube, and place it in the constant temperature incubator. Take it out after 24 hours and observe whether the hand cream has stratification.

[0129] Results: The paste remained homogeneous after returning to room temperature.

[0130] 1.4 Light stability test

[0131] Weigh hand cream samples of the same mass and place them in groups of 3 under natural light and dark conditions for 1, 2, 3, 4, and 5 hours respectively, and observe whether the hand cream changes color, odor, delamination, or other abnormal phenomena.

[0132] Result: No discoloration, odor change, delamination or other abnormal phenomena were observed.

[0133] 1.5 Moisturizing performance test

[0134] Moisturizing effect is one of the evaluation indicators of skin care products. We accurately weighed 5g of each hand cream sample and placed it in a weighing bottle. Each group of three samples was placed in an incubator at a relative humidity of 50% and a temperature of 25°C for 12, 24, 36, and 48 hours. The water loss rate of the hand cream was calculated according to formula (1). The lower the water loss rate, the better the moisturizing effect.

[0135] In addition, according to the formulation composition in Table 11, a blank matrix formulation without hippocampal peptide (ie, without adding hippocampal peptide) was prepared for comparative study of moisturizing performance.

[0136] Water loss rate = (M0-Mn) / M0×100% (1)

[0137] Where M0 is the original mass of the sample, g; Mn is the mass of the sample after being placed for n hours, g.

[0138] Table 16 Hand cream moisturizing performance test results

[0139] sample 12h 24h 36h 48h Hippocampal peptide hand cream sample 1 1.58％ 3.16％ 5.13％ 7.71％ Hippocampal peptide hand cream sample 2 1.98％ 3.75％ 5.93％ 8.31％ Hippocampal peptide hand cream sample 3 1.39％ 3.78％ 5.79％ 7.56％ Blank matrix sample 1 3.17％ 6.81％ 9.73％ 13.81％ Blank matrix sample 2 3.55％ 7.12％ 10.27％ 14.09％ Blank matrix sample 3 3.03％ 6.58％ 9.61％ 13.50％

[0140] From the results, we can see that the 48h moisturizing rate of hippocampal peptide hand cream is significantly better than that of blank matrix hand cream.

[0141] 2. Efficacy evaluation

[0142] The efficacy of hippocampal peptide hand cream and blank matrix hand cream (the same blank matrix hand cream prepared in the test of item 1.5 under Example 3) was compared. Thirty volunteers aged 18 to 60 with healthy skin were randomly divided into two groups, 15 people per group. Each group was given hippocampal peptide hand cream and blank matrix hand cream for a trial period of 4 weeks. The moisture, oil content, elasticity, and whiteness of the hand skin were tested before and after use of the hand cream using an intelligent skin tester. The test results are shown in Table 17.

[0143] Table 17 Efficacy evaluation results

[0144]

[0145] Note: Compared with the pre-trial group of the same sample, *P<0.05, **P<0.01, ***P<0.001

[0146] From the results in the above table, it can be seen that hippocampal peptide hand cream can significantly improve the moisture value, elasticity value and whiteness of the test subjects' hand skin, and has no effect on the oil content of the test subjects' hand skin; while the blank matrix hand cream can only significantly improve the moisture value of the test subjects' hand skin, and the increase is weaker than that of hippocampal peptide hand cream.

[0147] 3. Security Testing

[0148] Thirty volunteers aged 18 to 60 with healthy skin were randomly selected to trial the hand cream. Safety testing was conducted according to the human safety trial test specified in the 2022 edition of the "Technical Specifications for Safety of Cosmetics". The trial period was four weeks, and safety assessments were made according to Table 18 below. At the end of the trial, all participants had a skin reaction grade of 0, meaning "no reaction."

[0149] Table 18 Skin reaction grading standards for human trial tests

[0150] Skin reactions Grading No response 0 Faint erythema 1 Erythema, infiltration, papules 2 Erythema, edema, papules, blisters 3 Erythema, edema, bullae 4

[0151] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A hippocampal peptide hand cream, characterized in that: Contains the following components in percentage by mass, and the sum of the contents of each component is 100%: Hippocampal peptide: 2.5-17.5%, of which protein content (on dry basis) ≥80%, peptide content (on dry basis) ≥98%, and the proportion of protein hydrolysates with a relative molecular mass of peptides less than 500 ≥95%; Emulsifying system: Span 80, Tween 80, glyceryl stearate, stearic acid and cetyl alcohol, wherein the mass ratio of Span 80 to Tween 80 is 1: (1-3.5), and the mass ratio of stearic acid to cetyl alcohol is 1: (0.5-1.5); Moisturizer: glycerin 8-12%; Emollient: jojoba oil 4-6%, lanolin and petrolatum mass ratio (0.5-3):1; Fragrance: rose essential oil 0.1-0.3%; Preservative: sodium benzoate 0.2%; Solvent: deionized water; The preparation method of the hand cream comprises: (a) melting and dispersing Span 80, stearic acid, glyceryl stearate, lanolin, cetyl alcohol, jojoba oil, and vaseline at 70°C to prepare component A; (b) stirring and dissolving Tween 80, glycerol, and deionized water at 70°C to prepare component B; (c) slowly pouring component A into component B, stirring and emulsifying at 70-80°C and 800-1200 r / min for 3-11 minutes; and (d) cooling the mixture to 40°C, adding hippocampal peptide, rose essential oil, and sodium benzoate, and stirring and cooling the mixture to room temperature.

2. The hippocampal peptide hand cream according to claim 1, characterized in that: In the emulsification system, the mass ratio of Span 80 to Tween 80 is 2.5:2.5, and the mass ratio of stearic acid to cetyl alcohol is 1:1.

5.

3. The hippocampal peptide hand cream according to claim 1, characterized in that: The amount of glycerin used is 8%, the amount of jojoba oil used is 4%, and the amount of rose essential oil used is 0.2%.

4. A method for preparing the hippocampal peptide hand cream according to any one of claims 1 to 3, characterized in that: The process parameters of step (c) are: emulsification temperature 75° C., stirring speed 1000 r / min, and emulsification time 7 minutes.

5. Use of the hippocampal peptide hand cream according to any one of claims 1 to 3 for non-therapeutic improvement of moisture, elasticity and whiteness of hand skin.

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