Traditional Chinese medicine composition with soothing and repairing effects, external preparation and cosmetic

Abstract

The invention relates to a traditional Chinese medicine composition with soothing and repairing effects, an external preparation and cosmetics, the traditional Chinese medicine composition is prepared from radix ampelopsis, herba elsholtziae, radix angelicae sinensis and uncaria catechu, the radix ampelopsis comprises radix ampelopsis medicinal materials and / or radix ampelopsis extracts, the herba elsholtziae comprises herba elsholtziae medicinal materials and / or herba elsholtziae extracts, and the radix ampelopsis medicinal materials comprise radix ampelopsis medicinal materials and / or herba elsholtziae extracts. The angelica dahurica comprises an angelica dahurica medicinal material and / or an angelica dahurica extract, and the uncaria rhynchophylla comprises an uncaria rhynchophylla medicinal material and / or an uncaria rhynchophylla extract. According to the traditional Chinese medicine composition disclosed by the invention, through reasonable and scientific compatibility of all the components and in combination with the formula idea of monarch, minister, assistant and guide in traditional Chinese medicine, synergistic interaction of the formula is realized, excellent anti-inflammatory, anti-sensitive and anti-oxidation efficacy activity is exerted, and the traditional Chinese medicine composition is mild and non-irritant, can comprehensively relieve and repair skin, and effectively solves the skin health problem of people with sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine compositions, and in particular to a traditional Chinese medicine composition, an external preparation, and a cosmetic with soothing and repairing effects. Background Art

[0002] With the gradual improvement of living standards and increasing stress, the number of people suffering from sensitive skin is increasing. Symptoms include redness, itching, and acne, which can have a significant impact on physical and mental health. While some current soothing and repairing topical products offer rapid treatment or repair, they can also damage the skin barrier, leading to recurrence or worsening of symptoms. These skincare products also contain chemical ingredients such as preservatives, which can irritate the skin. Consequently, consumers are increasingly concerned about the source of their ingredients. Compared to chemically synthesized ingredients, Chinese herbal ingredients are natural and safe, aligning with the current trend towards environmental sustainability and gaining popularity. However, the complex molecular structures of Chinese herbal active ingredients result in low transdermal absorption, making them difficult to effectively penetrate deep into the skin to exert their effects. Consequently, the utilization rate of the active ingredients is low, making it difficult to guarantee the soothing and repairing effects of Chinese herbal ingredients. Furthermore, products containing Chinese herbal ingredients are less stable and are susceptible to environmental influences, leading to precipitation and other phenomena, which can affect the product's efficacy. Therefore, developing highly active and stable Chinese herbal ingredients remains a crucial goal for addressing the skin health issues faced by people with sensitive skin. Summary of the Invention

[0003] Based on this, it is necessary to provide a Chinese medicine composition with soothing and repairing effects, high stability, safety and non-irritation to solve the skin health problems of people with sensitive skin.

[0004] In the first aspect, the present application provides a traditional Chinese medicine composition with soothing and repairing effects, wherein the composition includes white ampelopsis, licorice root, angelica sinensis and Uncaria catechu, wherein the white ampelopsis includes white ampelopsis medicinal materials and / or white ampel extracts, the licorice root includes licorice medicinal materials and / or licorice extracts, the angelica sinensis includes angelica sinensis medicinal materials and / or angelica sinensis extracts, and the Uncaria catechu includes Uncaria catechu medicinal materials and / or Uncaria catechu extracts.

[0005] In some embodiments, the mass content of total polyphenols in the white ampelopsis is ≥8%; and / or the mass content of total flavonoids in the calendula is ≥5%; and / or the mass content of coumarins in the angelica sinensis is ≥3%; and / or the mass content of catechins in the Uncaria catechu is ≥15%.

[0006] In some embodiments, the Chinese medicine composition further satisfies at least one of the following characteristics (1) to (6):

[0007] (1) In parts by mass, the composition of the Chinese medicine composition includes 10-20 parts of Bletilla striata, 8-20 parts of Elsholtzia ciliata, 1-10 parts of Angelica sinensis and 1-10 parts of Uncaria catechu;

[0008] (2) The Chinese medicine composition further comprises a solvent;

[0009] (3) extracting the Bletilla striata medicinal material with an extraction solvent to prepare the Bletilla striata extract;

[0010] (4) extracting the Radix Angelicae Sinensis medicinal material with an extraction solvent to prepare the Radix Angelicae Sinensise extract;

[0011] (5) performing supercritical extraction on the Elsholtzia ciliata medicinal material to prepare the Elsholtzia ciliata extract;

[0012] (6) The Uncaria rhynchophylla catechu extract is prepared by supercritical extraction of the Uncaria rhynchophylla catechu.

[0013] In some embodiments, the Chinese herbal composition with soothing and repairing effects further satisfies at least one of the following (1) to (10):

[0014] (1) The solvent includes one or more of polyol and water;

[0015] (2) The ratio of the total mass of the white ampelopsis, the scutellaria baicalensis, the angelica sinensis and the catechu Uncaria rhynchophylla to the mass of the solvent is (0.5-1.5): (50-70);

[0016] (3) The extraction solvent includes one or more of water and an organic solvent;

[0017] (4) The mass ratio of the white ampelopsis medicinal material and the Angelica sinensis medicinal material to the extraction solvent is 1: (10-20);

[0018] (5) The pressure of supercritical extraction is 20MPa-40MPa;

[0019] (6) The temperature of supercritical extraction is 40℃-50℃;

[0020] (7) The separation pressure of supercritical extraction is 5MPa-10MPa;

[0021] (8) The separation temperature of supercritical extraction is 30℃-70℃;

[0022] (9) The solvent for supercritical extraction is one or more of water and organic solvent;

[0023] (10) Supercritical extraction includes supercritical CO2 extraction.

[0024] In a second aspect, the present application provides a method for preparing the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect, comprising the following steps:

[0025] The Chinese medicinal composition with soothing and repairing effects is prepared by mixing white ampelopsis, licorice root, angelica sinensis and Uncaria catechu.

[0026] In some embodiments, microfluidization is used during the mixing process, the operating temperature of the microfluidization is 0° C.-70° C., the operating pressure of the microfluidization is 1000 psi-30000 psi; and / or, a solvent is added during the mixing process.

[0027] In a third aspect, the present application provides an external preparation, the ingredients of which include the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect or the traditional Chinese medicine composition with soothing and repairing effects prepared by the preparation method provided in the second aspect, and excipients.

[0028] In some embodiments, the mass addition amount of the Chinese herbal composition with soothing and repairing effects in the external preparation is 0.5%-10%; and / or, the excipients include one or more of fillers, diluents, lubricants, wetting agents and adhesives; and / or, the dosage form of the external preparation includes powders, ointments, pills, wines, tablets, aqueous solutions, aerosols or papules.

[0029] In a fourth aspect, the present application provides a cosmetic, the ingredients of which include the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect or the traditional Chinese medicine composition with soothing and repairing effects prepared by the preparation method provided in the second aspect, and additives.

[0030] In some embodiments, the cosmetic further satisfies at least one of the following characteristics (1) to (4):

[0031] (1) The amount of the Chinese medicine composition added to the cosmetic is 0.5%-10% by mass;

[0032] (2) The additives include one or more of a moisturizer, a surfactant, an antioxidant, and a stabilizer;

[0033] (3) The cosmetic is in the form of a cream, liquid, emulsion or foam;

[0034] (4) The cosmetics include essence, cream, mask or cleanser.

[0035] Compared with traditional technologies, the advantages of this application include:

[0036] The present application provides a Chinese medicine composition with soothing and repairing effects, which achieves synergistic effect of the prescription by rationally and scientifically combining Bletilla striata, Elsholtzia ciliata, Angelica sinensis with Dianthus dentata and Uncaria catechu, and combining the prescription concept of "monarch, minister, assistant and envoy" in traditional Chinese medicine. The Chinese medicine composition with soothing and repairing effects provided by the present application has excellent anti-inflammatory, anti-allergic and antioxidant activities, is mild and non-irritating, and can soothe and repair the skin in an all-round way. At the same time, the Chinese medicine composition with soothing and repairing effects provided by the present application has excellent permeability and stability, which can further exert the synergistic effect between the components and improve the storage performance of the Chinese medicine composition. DETAILED DESCRIPTION

[0037] To facilitate understanding of the present application, the present application will be described more fully below with reference to the relevant embodiments. Preferred embodiments of the present application are provided in the embodiments. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0039] As used herein, “optionally”, “optional” and “optional” refer to any one of the two parallel schemes of “with” or “without”. If multiple “optional” appear in a technical solution, unless otherwise specified and there is no contradiction or mutual restriction, each “optional” is independent of each other. The term “and / or” used herein includes any and all combinations of one or more related listed items. “Multiple”, “multiple” and the like involved in this article, unless otherwise specified, refer to a quantity greater than 2 or equal to 2, for example, “one or more” means one, two or more than two. In the open technical features or technical solutions described herein with words such as “contains”, “includes” and “includes”, unless otherwise specified, additional members other than the listed members are not excluded, and it can be regarded as providing both closed features or solutions consisting of the listed members and open features or solutions that also include additional members in addition to the listed members.

[0040] In this application, the terms "first," "second," "third," "fourth," etc. in "the first aspect," "the second aspect," "the third aspect," "the fourth aspect," etc. are used for descriptive purposes only and should not be understood as indicating or implying relative importance or quantity, nor should they be understood as implicitly indicating the importance or quantity of the indicated technical features. Furthermore, "first," "second," "third," "fourth," etc. serve only as non-exhaustive enumeration and description, and should be understood not to constitute a closed-ended limitation on quantity.

[0041] Unless otherwise specified, all embodiments and optional embodiments of the present application can be combined with each other to form a new technical solution.

[0042] Traditional medicines or skin care products for skin treatment or repair contain some chemically synthesized ingredients, which have certain irritation. They are also prone to damage to the skin barrier during treatment and repair, and are prone to repeated and deepening inflammation. Therefore, more and more consumers pay more attention to products with natural active ingredients. However, the active ingredient structure of natural active ingredients is complex, and the transdermal absorption rate is low, making it difficult to effectively penetrate into the deep layer of the skin to exert its effect. In the process of treatment and repair, the time is long and the efficacy is limited. At the same time, the synergistic efficacy activity is weak, resulting in a single efficacy activity, which is difficult to meet the needs of daily life. The present application aims to provide a highly active Chinese medicine composition that repairs the skin barrier, while also having diversified efficacy activities, such as Chinese medicine compositions with antioxidant, anti-allergic and other effects.

[0043] In the first aspect, the present application provides a traditional Chinese medicine composition with soothing and repairing effects, wherein the composition includes white ampelopsis, licorice root, angelica sinensis and Uncaria catechu, wherein the white ampelopsis includes white ampelopsis medicinal materials and / or white ampel extracts, the licorice root includes licorice medicinal materials and / or licorice extracts, the angelica sinensis includes angelica sinensis medicinal materials and / or angelica sinensis extracts, and the Uncaria catechu includes Uncaria catechu medicinal materials and / or Uncaria catechu extracts.

[0044] The Chinese herbal composition of this application includes Radix Angelicae Pubescentis as the main ingredient, primarily playing a leading role in antioxidant activity. Its rich polyphenol content can effectively scavenge free radicals and delay skin aging, providing fundamental support for its core efficacy. The Chinese herbal composition of this application includes Radix Echeveriae as a secondary ingredient, synergizing with the main ingredient, Radix Echeveriae, to exert its anti-pathogenic effect. Its antibacterial properties can effectively inhibit harmful bacteria on the skin surface and prevent skin infections. Through a reasonable proportion, the synergistic effect of Radix Echeveriae and Radix Echeveriae can significantly enhance the overall efficacy of cosmetic ingredients, enhancing the product's antibacterial and antioxidant capabilities, and providing more comprehensive protection for skin health. The Chinese herbal composition of this application includes Radix Angelicae Sinensis as an adjuvant, playing a synergistic and coordinating role in the composition. Its microcirculation-promoting properties can enhance the skin's nutrient supply and metabolic capacity, further enhancing its antioxidant and anti-aging effects. Furthermore, Radix Angelicae Sinensis Sinensis can reduce the irritation of other ingredients, making the entire Chinese herbal composition gentler, reducing adverse reactions to the skin, and improving product safety. The addition of catechu as an adjuvant to the Chinese medicine composition of this application is primarily responsible for regulating the pH value of the formula and the stability of the dosage form. This ingredient can maintain the pH value of the cosmetic raw materials within an appropriate range, ensuring the stability and safety of the product. Catechu also has a certain astringent effect, which can shrink skin pores, making the skin more delicate and smooth, further enhancing the product's use effect and sensory experience.

[0045] The Chinese medicine composition provided in this application achieves synergistic enhancement of the prescription through reasonable and scientific proportioning of Chinese medicine prescriptions, combined with the prescription concept of "monarch, minister, assistant and envoy" in traditional Chinese medicine. Through the synergistic effects of white ampelopsis, stone calendula, angelica sinensis and Uncaria catechu, the skin barrier repair function activity of the Chinese medicine composition is significantly improved, the penetration performance of the ingredients of the composition is improved, and synergistic multi-functional activity is produced to meet people's daily skin care needs.

[0046] Controlling the content of each ingredient in the Chinese medicine composition within a certain range is the core element to ensure its safety, effectiveness and quality stability. This process involves multidisciplinary theories such as Chinese medicine pharmacology, pharmacy, and quality control. This application can better achieve the synergistic effect of each ingredient in the formula by controlling the content of Bletilla striata, Elsholtzia ciliata, Angelica sinensis and Uncaria catechu within a certain range, thereby further obtaining higher skin barrier repair efficacy and activity.

[0047] In some embodiments, the mass content of total polyphenols in the white peony root in the traditional Chinese medicine composition is ≥8%. When the mass content of total polyphenols in the white peony root is ≥8%, the traditional Chinese medicine composition of the present application has a more active skin barrier repair effect, can better scavenge free radicals, delay skin aging, and give full play to its role as the main medicine in the traditional Chinese medicine composition. As a non-limiting example, the mass content of total polyphenols in the white peony root in the traditional Chinese medicine composition is ≥8%, including the mass content of total polyphenols in the white peony root medicinal material ≥8%, or the mass content of total polyphenols in the white peony root extract ≥8%, or the mass content of total polyphenols in the mixture of the white peony root medicinal material and the white peony root extract ≥8%. Further, the mass content of total polyphenols in the white peony root in the traditional Chinese medicine composition of the present application is 8%-24%, within which the technical effect of the present application can be better achieved. As a non-limiting example, the mass content of total polyphenols in the white peony root medicinal material and / or the white peony root extract in the traditional Chinese medicine composition of the present application is 8%-24%. Including but not limited to 8%, 12%, 16%, 20%, 24% or the range formed by any two of the foregoing and the values ​​within the range.

[0048] In some embodiments, the mass content of the total flavonoids in the Herba Cyperi in the Chinese medicine composition is ≥5%. When the mass content of the total flavonoids in the Herba Cyperi is ≥5%, it can better produce a synergistic effect of skin barrier repair with the Herba Cyperi with improved activity. At the same time, when the mass content of the total flavonoids in the Herba Cyperi is ≥5%, it can produce better antibacterial and antioxidant activity, inhibit harmful bacteria on the skin surface, prevent skin infection, promote skin repair, and give full play to its role as a ministerial drug in the Herba Cyperi formula. As a non-limiting example, the mass content of the total flavonoids in the Herba Cyperi in the Chinese medicine composition is ≥5%, including the mass content of the total flavonoids in the Herba Cyperi medicinal material ≥5%, or the mass content of the total flavonoids in the Herba Cyperi extract ≥5%, or the mass content of the total flavonoids in the Herba Cyperi medicinal material and the Herba Cyperi extract ≥5%. Further, the mass content of the total flavonoids in the Herba Cyperi in the Chinese medicine composition of the present application is 5%-15%, within which the technical effects of the present application can be better achieved. As a non-limiting example, the total flavonoids content of the Herba Elsholtziae medicinal materials and / or the Herba Elsholtziae extract in the traditional Chinese medicine composition of the present application is 5%-15% by weight, including but not limited to 5%, 7%, 9%, 11%, 13%, 15%, or a range formed by any two of the foregoing, and values ​​within the range.

[0049] In some embodiments, the mass content of the coumarin components in the Radix Angelicae Dahuricae in the Chinese medicine composition is ≥3%. When the mass content of the coumarin components in the Radix Angelicae Dahuricae is ≥3%, it can better produce a synergistic effect of skin barrier repair with white peony root and scutellaria baicalensis, while also reducing the irritation of the Chinese medicine composition, making the Chinese medicine composition milder. When the mass content of the coumarin components in the Radix Angelicae Dahuricae is ≥3%, it can promote microcirculation, thereby enhancing the nutrient supply and metabolic capacity of the skin, further enhancing the antioxidant and anti-aging effects, promoting skin repair, and giving full play to its adjuvant role in the formulation of the Chinese medicine composition. As a non-limiting example, the mass content of the coumarin components in the Radix Angelicae Dahuricae in the Chinese medicine composition is ≥3%, including the mass content of the coumarin components in the Radix Angelicae Dahuricae medicinal material ≥3%, or the mass content of the coumarin components in the Radix Angelicae Dahuricae extract is ≥3%, or the mass content of the coumarin components in the mixture of the Radix Angelicae Dahuricae medicinal material and the Radix Angelicae Dahuricae extract is ≥3%. Furthermore, the mass content of the coumarin components in the Radix Angelicae Dahuricae in the Chinese medicine composition of the present application is 3%-10%, and within this range, the technical effects of the present application can be better achieved. As a non-limiting example, the mass content of the coumarin components in the Radix Angelicae Dahuricae medicinal material and / or the Radix Angelicae Dahuricae extract in the Chinese medicine composition of the present application is 3%-10%. Including but not limited to the range formed by 3%, 5%, 7%, 9%, 10% or any two of the foregoing and the values ​​within the range.

[0050] In some embodiments, the mass content of catechins in the Uncaria catechu in the Chinese medicine composition is ≥15%. When the mass content of catechins in the Uncaria catechu is ≥15%, it can better produce a synergistic effect of skin barrier repair with white ampelopsis and scutellaria baicalensis, while also reducing the irritation of the Chinese medicine composition, making the Chinese medicine composition milder. When the mass content of catechins in the Uncaria catechu is ≥3%, it can promote microcirculation, thereby enhancing the nutrient supply and metabolic capacity of the skin, further enhancing the antioxidant and anti-aging effects, promoting skin repair, and giving full play to its adjuvant role in the Chinese medicine composition. As a non-limiting example, the mass content of catechins in the Uncaria catechu in the Chinese medicine composition is ≥15%, including the mass content of catechins in the Uncaria catechu medicinal material ≥15%, or the mass content of catechins in the Uncaria catechu extract ≥15%, or the mass content of catechins in the mixture of the Uncaria catechu medicinal material and the Uncaria catechu extract ≥15%. Furthermore, the mass content of catechins in the Uncaria catechu in the Chinese medicine composition of the present application is 15%-25%, and within this range, the technical effects of the present application can be better achieved. As a non-limiting example, the mass content of catechins in the Uncaria catechu medicinal material and / or the Uncaria catechu extract in the Chinese medicine composition of the present application is 15%-25%, including but not limited to 15%, 17%, 19%, 21%, 25%, or the range formed by any two of the foregoing, and values ​​within the range.

[0051] In some embodiments, the Chinese medicine composition comprises, by weight, 10-20 parts of Bletilla striata, 8-20 parts of Elsholtzia ciliata, 1-10 parts of Angelica sinensis and 1-10 parts of Uncaria catechu.

[0052] In some embodiments, the Chinese medicine composition comprises, by weight, 10-15 parts of Bletilla striata, 8-15 parts of Elsholtzia ciliata, 3-8 parts of Angelica sinensis and 1-5 parts of Uncaria catechu.

[0053] In some embodiments, the Chinese medicine composition comprises, by weight, 10-15 parts of Bletilla striata, 8-12 parts of Elsholtzia ciliata, 4-6 parts of Angelica sinensis and 2-4 parts of Uncaria catechu.

[0054] In some embodiments, the composition of the traditional Chinese medicine composition also includes a solvent.

[0055] In some embodiments, the solvent includes one or more of a polyol and water. As non-limiting examples, the polyol includes one or more of butanediol, 1,2-propylene glycol, glycerol, and 1,3-propylene glycol.

[0056] In some embodiments, the ratio of the total weight of the Bletilla striata, Elsholtzia ciliata, Angelica sinensis and Uncaria catechu to the solvent is (0.5-1.5):(50-70), including but not limited to 0.5:50, 0.5:60, 0.5:70, 1:50, 1:60, 1:70, 1.5:50, 1.5:60, 1.5:70, or a range formed by any two of the foregoing, and values ​​within the range.

[0057] In the traditional Chinese medicine composition of the present application, the type and content of the active ingredients contained in each component play an important role in the skin barrier repair effect of the traditional Chinese medicine composition of the present application.

[0058] The extraction method of Chinese herbal medicine extracts has a significant impact on the composition and efficacy of the extracts. The extraction methods of the Chinese herbal medicine extracts in the Chinese medicine composition described in this application are as follows:

[0059] In some embodiments, the Bletilla striata extract is prepared by extracting Bletilla striata medicinal materials with an extraction solvent.

[0060] In some embodiments, the extraction solvent includes one or more of water and an organic solvent, wherein the organic solvent includes an alcohol solvent. The Bletilla striata extract includes a water extract of Bletilla striata or an alcohol extract of Bletilla striata.

[0061] As a non-limiting example, the alcoholic solvent includes ethanol. In some embodiments, the mass fraction of the ethanol is 70%-80%. As a non-limiting example, it includes but is not limited to 70%, 72%, 75%, 78%, 80% or the range formed by any two of the foregoing and the values ​​within the range.

[0062] In some embodiments, the mass ratio of the material to the liquid during the extraction process (the mass ratio of the Bletilla striata medicinal material to the extraction solvent) is 1:(10-20). Non-limiting examples include, but are not limited to, 1:10, 1:12, 1:15, 1:18, 1:20, or a range formed by any two of the foregoing, and values ​​within the range.

[0063] In some embodiments, the extraction time is 1 hour to 3 hours. As non-limiting examples, the extraction time includes but is not limited to 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, or a range formed by any two of the foregoing, and values ​​within the range.

[0064] In some embodiments, the number of extractions is 1-3 times. As non-limiting examples, the number includes but is not limited to 1 time, 2 times, 3 times, or a range formed by any two of the foregoing, and values ​​within the range.

[0065] In some embodiments, the extraction process further includes a purification process in which the extract is subjected to column chromatography. As a non-limiting example, the column chromatography comprises a macroporous resin, including D101 and / or AB-8 macroporous resin.

[0066] In some embodiments, the elution solvent for column chromatography comprises one or more of methanol, ethanol, ethyl acetate, isopropanol, and acetone.

[0067] In some embodiments, the extract is freeze-dried to better ensure the activity of the active ingredients in the extract.

[0068] In some embodiments, the Angelica sinensis extract is prepared by extracting the Angelica sinensis medicinal material with an extraction solvent.

[0069] In some embodiments, the extraction solvent comprises one or more of water and an organic solvent, wherein the organic solvent comprises an alcohol solvent. The Angelica sinensis extract comprises an Angelica sinensis water extract or an Angelica sinensis alcohol extract.

[0070] As a non-limiting example, the alcoholic solvent includes ethanol. In some embodiments, the mass fraction of the ethanol is 70%-80%. As a non-limiting example, it includes but is not limited to 70%, 72%, 75%, 78%, 80% or the range formed by any two of the foregoing and the values ​​within the range.

[0071] As a non-limiting example, the alcohol extraction is ethanol extraction. In some embodiments, the mass fraction of the ethanol is 70%-80%. As a non-limiting example, it includes but is not limited to 70%, 72%, 75%, 78%, 80% or the range formed by any two of the foregoing and the numerical value within the range.

[0072] In some embodiments, the material-to-liquid mass ratio (mass ratio of the Angelica sinensis herb to the extraction solvent) during the extraction process is 1:(10-20). Non-limiting examples include, but are not limited to, 1:10, 1:12, 1:15, 1:18, 1:20, or a range formed by any two of the foregoing, and values ​​within those ranges.

[0073] In some embodiments, the extraction time is 1 hour to 3 hours. As non-limiting examples, the extraction time includes but is not limited to 1 hour, 1.5 hours, 2 hours, 2.5 hours, 3 hours, or a range formed by any two of the foregoing, and values ​​within the range.

[0074] In some embodiments, the number of extractions is 1-3 times. As non-limiting examples, the number includes but is not limited to 1 time, 2 times, 3 times, or a range formed by any two of the foregoing, and values ​​within the range.

[0075] In some embodiments, the extract is freeze-dried to better ensure the activity of the active ingredients in the extract.

[0076] In some embodiments, the Herba Elsholtziae extract includes the Herba Elsholtziae supercritical extract. The Herba Elsholtziae supercritical extract is prepared by supercritical extraction of Herba Elsholtziae medicinal materials. As a non-limiting example, the Herba Elsholtziae supercritical extract includes the Herba Elsholtziae supercritical CO2 extract.

[0077] In some embodiments, the pressure of supercritical extraction of Elsholtzia ciliata medicinal material is 20 MPa-40 MPa, including but not limited to 20 MPa, 22 MPa, 25 MPa, 28 MPa, 30 MPa, 35 MPa, 40 MPa, or a range formed by any two of the foregoing, and values ​​within the range.

[0078] In some embodiments, the temperature for supercritical extraction of Elsholtzia ciliata is 40-50° C. As non-limiting examples, the temperature includes but is not limited to 40° C., 42° C., 45° C., 48° C., 50° C., or a range formed by any two of the foregoing, and values ​​within the range.

[0079] In some embodiments, the separation temperature of the supercritical extraction of Elsholtzia ciliata medicinal material is 30° C.-70° C. As non-limiting examples, the temperature includes but is not limited to 30° C., 35° C., 40° C., 45° C., 50° C., 55° C., 60° C., 65° C., 70° C., or a range formed by any two of the foregoing, and values ​​within the range.

[0080] In some embodiments, the separation pressure of the supercritical extraction of the Herba Elsholtziae is 5 MPa-10 MPa, including but not limited to 5 MPa, 6 MPa, 7 MPa, 8 MPa, 9 MPa, 10 MPa, or a range formed by any two of the foregoing, and values ​​within the range.

[0081] In some embodiments, the solvent for supercritical extraction of the Herba Cynoglossi medicinal materials is one or more of water and an organic solvent. Further, the solvent for supercritical extraction of the Herba Cynoglossi medicinal materials of the present application is an organic solvent. As a non-limiting example, the solvent is ethanol. As a non-limiting example, the mass fraction of the ethanol is 70%-80%, including but not limited to 70%, 72%, 75%, 78%, 80% or the range formed by any two of the foregoing and the values ​​within the range.

[0082] In some embodiments, the extract prepared by supercritical extraction of the Elsholtzia ciliata medicinal material is subjected to low-temperature freeze-drying, which can better ensure the activity of the active ingredients in the extract.

[0083] In some embodiments, the supercritical extract of Uncaria catechu includes a supercritical extract of Uncaria catechu. The supercritical extract of Uncaria catechu is prepared by supercritical extraction of Uncaria catechu. As a non-limiting example, the supercritical extract of Uncaria catechu includes a supercritical CO2 extract of Elsholtzia oleifera.

[0084] In some embodiments, the pressure of the supercritical extraction of Uncaria rhynchophylla catechu is 20 MPa-40 MPa, including but not limited to 20 MPa, 22 MPa, 25 MPa, 28 MPa, 30 MPa, 35 MPa, 40 MPa, or a range formed by any two of the foregoing, and values ​​within the range.

[0085] In some embodiments, the temperature for supercritical extraction of Uncaria catechu is 40-50° C. As non-limiting examples, the temperature includes but is not limited to 40° C., 42° C., 45° C., 48° C., 50° C., or a range formed by any two of the foregoing, and values ​​within the range.

[0086] In some embodiments, the separation temperature of the supercritical extraction of Uncaria rhynchophylla catechu is 30° C.-70° C. As non-limiting examples, the separation temperature includes but is not limited to 30° C., 35° C., 40° C., 45° C., 50° C., 55° C., 60° C., 65° C., 70° C., or a range formed by any two of the foregoing, and values ​​within the range.

[0087] In some embodiments, the separation pressure of the supercritical extraction of Uncaria rhynchophylla catechu is 5 MPa-10 MPa, including but not limited to 5 MPa, 6 MPa, 7 MPa, 8 MPa, 9 MPa, 10 MPa, or a range formed by any two of the foregoing, and values ​​within the range.

[0088] In some embodiments, the solvent for supercritical extraction of the catechu and Uncaria rhynchophylla medicinal material is one or more of water and an organic solvent. Further, the solvent for supercritical extraction of the catechu and Uncaria rhynchophylla medicinal material of the present application is an organic solvent. As a non-limiting example, the solvent is ethanol. As a non-limiting example, the mass fraction of the ethanol is 70%-80%, including but not limited to 70%, 72%, 75%, 78%, 80% or the range formed by any two of the foregoing and the values ​​within the range.

[0089] In some embodiments, the extract prepared by supercritical extraction of the Uncaria catechu medicinal material is subjected to low-temperature freeze-drying, which can better ensure the activity of the active ingredients in the extract.

[0090] In a second aspect, the present application provides a method for preparing the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect, comprising the following steps:

[0091] The Chinese medicinal composition with soothing and repairing effects is prepared by mixing white ampelopsis, licorice root, angelica sinensis and Uncaria catechu.

[0092] In some embodiments, a solvent is added for mixing during the preparation of the traditional Chinese medicine composition with soothing and repairing effects.

[0093] In some embodiments, microfluidization is used during the mixing process. The present application uses microfluidization equipment to treat the mixed materials, which can disperse the Chinese medicine composition to the nanometer level, significantly improve the uniformity and stability of the Chinese medicine composition, and improve the penetration performance of the Chinese medicine composition during the application process, thereby better exerting the functional activity of the Chinese medicine composition.

[0094] In some embodiments, the operating temperature of the microfluidization process is 0°C-70°C. Non-limiting examples include, but are not limited to, 0°C, 5°C, 8°C, 10°C, 12°C, 15°C, 20°C, 25°C, 30°C, 40°C, 50°C, 60°C, 70°C, or any range formed by two of the foregoing, and values ​​within these ranges. Furthermore, the microfluidization process of the present application can better achieve the technical effects of the present application by using an operating temperature of 10°C.

[0095] In some embodiments, the microfluidization process operates at a pressure of 1000 psi to 30,000 psi. Non-limiting examples include, but are not limited to, 1000 psi, 5000 psi, 10,000 psi, 15,000 psi, 20,000 psi, 30,000 psi, or any range formed by two of the foregoing, and values ​​within these ranges. Furthermore, the microfluidization process of the present application utilizes an operating pressure of 15,000 psi to better achieve the technical effects of the present application.

[0096] In a third aspect, the present application provides an external preparation, the ingredients of which include the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect or the traditional Chinese medicine composition with soothing and repairing effects prepared by the preparation method provided in the second aspect, and excipients.

[0097] In some embodiments, the amount of the soothing and restorative Chinese medicine composition added to the topical preparation is 0.5%-10%, including, but not limited to, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or any two of the foregoing, and values ​​within these ranges.

[0098] In some embodiments, the excipient includes one or more of a filler, a diluent, a lubricant, a wetting agent, and a binder. As a non-limiting example, the excipient can be a pharmaceutically acceptable excipient.

[0099] In some embodiments, the dosage form of the external preparation includes powder, ointment, pill, wine, lozenge, solution, aerosol or papule.

[0100] The topical preparation provided in this application can be used for anti-inflammatory, anti-oxidation, skin barrier repair or inhibition of Propionibacterium acnes, etc., and has the effect of soothing the skin.

[0101] In a fourth aspect, the present application provides a cosmetic, the ingredients of which include the traditional Chinese medicine composition with soothing and repairing effects provided in the first aspect or the traditional Chinese medicine composition with soothing and repairing effects prepared by the preparation method provided in the second aspect, and additives.

[0102] In some embodiments, the amount of the soothing and restorative Chinese medicine composition added to the cosmetic is 0.5%-10%, including, but not limited to, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, or any two of the foregoing, and values ​​within these ranges.

[0103] In some embodiments, the additives include one or more of a humectant, a surfactant, an antioxidant, and a stabilizer.

[0104] In some embodiments, the cosmetic is in the form of a cream, liquid, emulsion or foam.

[0105] In some embodiments, the cosmetic comprises a serum, a cream, a mask, or a cleanser.

[0106] The cosmetics provided in this application can be used for anti-inflammatory, anti-oxidation, skin barrier repair or inhibition of Propionibacterium acnes, etc., and have the effect of soothing the skin.

[0107] For experimental parameters not specified in the following specific examples, reference is made to the guidance given in this application document, and reference is also made to experimental manuals in the field or other experimental methods known in the field, or to the experimental conditions recommended by the manufacturer.

[0108] The substrates and reagents involved in the following specific examples are commercially available or can be prepared by those skilled in the art according to known methods.

[0109] Preparation of Bletilla striata extract-1:

[0110] After the medicinal material of Bletilla striata was crushed, reflux extraction was carried out with an ethanol aqueous solution (ethanol content of 70wt%), the solid-liquid ratio (mass ratio) was 1:10, the extraction time was 2h, and the number of extractions was 2 times. The filter was filtered using a filter cloth and a filter membrane (0.45μm) in succession, and the filtrate was collected. It was then adsorbed and eluted with a D101 resin, and the elution solution was 50% ethanol for 3BV. Thereafter, the eluate was subjected to reduced pressure distillation using a rotary evaporator to remove ethanol, and the solid material was freeze-dried to prepare a Bletilla striata extract-1, wherein the mass content of total polyphenols in the Bletilla striata extract-1 is ≥8%.

[0111] Preparation of Bletilla striata Extract-2:

[0112] After the medicinal material of Bletilla striata was crushed, reflux extraction was performed with an ethanol aqueous solution (ethanol content of 75wt%), the solid-liquid ratio (mass ratio) was 1:10, the extraction time was 1h, and the number of extractions was 3. The product was filtered through a filter cloth and a filter membrane (0.45μm) in sequence, and the filtrate was collected. The filtrate was then subjected to reduced pressure distillation using a rotary evaporator to remove ethanol, and the solid material was then freeze-dried to prepare Bletilla striata extract-2. The mass content of total polyphenols in the Bletilla striata extract-2 was ≤8%.

[0113] Preparation of Angelica sinensis Extract-1:

[0114] The medicinal material of Angelica sinensis was crushed and reflux extracted with an ethanol solution (75wt%), the solid-liquid ratio (mass ratio) was 1:10, the extraction time was 1h, and the number of extractions was 3. The product was filtered using a filter cloth and a filter membrane (0.45μm) in succession, the filtrate was collected, and then adsorbed and eluted with an AB-8 resin, the elution solution was 50% ethanol, and the elution time was 3BV. The eluate was distilled under reduced pressure using a rotary evaporator to remove ethanol, and the solid material was freeze-dried to prepare an Angelica sinensis sinensis extract-1, wherein the mass content of coumarin components in the Angelica sinensis sinensis extract-1 is ≥3%.

[0115] Preparation of Angelica sinensis Extract-2:

[0116] The medicinal material of Angelica sinensis was crushed and reflux extracted with an ethanol solution (75wt%) at a solid-liquid ratio (mass ratio) of 1:10. The extraction time was 1h and the number of extractions was 3. The material was filtered using a filter cloth and a filter membrane (45μm) in succession, and the filtrate was collected. The filtrate was then subjected to reduced pressure distillation using a rotary evaporator to remove ethanol. The solid material was then freeze-dried to prepare an Angelica sinensis extract-2. The mass content of coumarin components in the Angelica sinensis extract-2 was ≤3%.

[0117] Preparation of Elsholtzia ciliata extract-1:

[0118] After the Herba Cyperi Herba is crushed, the Herba Cyperi Herba is placed in a supercritical CO2 extraction kettle, the extraction temperature is set to 50°C, the extraction pressure is set to 35MPa, the separation temperature is set to 60°C, the separation pressure is set to 10MPa, and the extraction solvent is an ethanol aqueous solution (ethanol content is 80wt%). After extraction, the filtrate is collected and the filtrate is subjected to reduced pressure distillation using a rotary evaporator to remove ethanol. The solid material is then freeze-dried to prepare the Herba Cyperi Herba Extract-1, wherein the mass content of total flavonoids in the Herba Cyperi Herba Extract-1 is ≥5%.

[0119] Preparation of Elsholtzia ciliata extract-2:

[0120] After the Herba Cyperi Herba is crushed, the Herba Cyperi Herba is placed in a supercritical CO2 extraction kettle, the extraction temperature is set to 50°C, the extraction pressure is set to 15MPa, the separation temperature is set to 60°C, the separation pressure is set to 10MPa, and the extraction solvent is an ethanol aqueous solution (ethanol content is 80wt%). After extraction, the filtrate is collected and the filtrate is subjected to reduced pressure distillation using a rotary evaporator to remove ethanol. The solid material is then freeze-dried to prepare the Herba Cyperi Herba Extract-2, wherein the mass content of total flavonoids in the Herba Cyperi Herba Extract-2 is ≤5%.

[0121] Preparation of Uncaria Catechu Extract-1:

[0122] The Uncaria catechu herb is crushed and placed in a supercritical CO2 extraction kettle. The extraction temperature is set to 50°C, the extraction pressure is set to 35MPa, the separation temperature is set to 60°C, the separation pressure is set to 10MPa, and the extraction solvent is an ethanol aqueous solution (ethanol content is 80wt%). After extraction, the filtrate is collected and the filtrate is subjected to reduced pressure distillation using a rotary evaporator to remove ethanol. The solid material is then freeze-dried to prepare the Uncaria catechu herb extract-1, and the mass content of catechin components in the Uncaria catechu herb extract-1 is ≥15%.

[0123] Preparation of Uncaria Catechu Extract-2:

[0124] The Uncaria catechu herb is crushed and placed in a supercritical CO2 extraction kettle. The extraction temperature is set to 50°C, the extraction pressure is set to 15 MPa, the separation temperature is set to 60°C, the separation pressure is set to 10 MPa, and the extraction solvent is an ethanol aqueous solution (ethanol content is 80wt%). After extraction, the filtrate is collected and the filtrate is subjected to reduced pressure distillation using a rotary evaporator to remove ethanol. The solid material is then freeze-dried to prepare the Uncaria catechu herb extract-2, and the mass content of catechin components in the Uncaria catechu herb extract-2 is ≤15%.

[0125] Example 1

[0126] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 1.12g of Bletilla striata extract, 1.12g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 1.2g of Uncaria catechu extract.

[0127] Example 2

[0128] The Chinese medicine composition with soothing and repairing effects of this embodiment includes: 1.12g of Bletilla striata extract, 1.10g of Elsholtzia ciliata extract, 1.5g of Angelica sinensis extract and 1.3g of Uncaria catechu extract.

[0129] Example 3

[0130] The Chinese medicine composition with soothing and repairing effects of this embodiment includes: 1.13g of Bletilla striata extract, 1.9g of Elsholtzia ciliata extract, 1.5g of Angelica sinensis extract and 1.3g of Uncaria catechu extract.

[0131] Example 4

[0132] The Chinese medicine composition with soothing and repairing effects of this embodiment includes: 1.10g of Bletilla striata extract, 1.10g of Elsholtzia ciliata extract, 1.6g of Angelica sinensis extract and 1.4g of Uncaria catechu extract.

[0133] Example 5

[0134] The Chinese medicine composition with soothing and repairing effects of this embodiment includes: 1.15g of Bletilla striata extract, 1.8g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 1.3g of Uncaria catechu extract.

[0135] Example 6

[0136] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 1.14g of Bletilla striata extract, 1.10g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 1.2g of Uncaria catechu extract.

[0137] Example 7

[0138] The Chinese medicine composition with soothing and repairing effects of this embodiment includes: 1.5g of Bletilla striata extract, 1.6g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 1.2g of Uncaria catechu extract.

[0139] Example 8

[0140] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 1.21g of Bletilla striata extract, 1.11g of Elsholtzia ciliata extract, 1.14g of Angelica sinensis extract and 1.0.2g of Uncaria catechu extract.

[0141] Example 9

[0142] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 1.12g of Ampelopsis pilosula extract, 1.12g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 2.2g of Uncaria catechu extract.

[0143] Example 10

[0144] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 2.12g of Ampelopsis pilosula extract, 1.12g of Elsholtzia ciliata extract, 2.4g of Angelica sinensis extract and 1.2g of Uncaria catechu extract.

[0145] The preparation method of the Chinese medicine composition with soothing and repairing effects of Examples 1 to 10 is:

[0146] According to the formula, the extract of Bletilla striata, the extract of Elsholtzia ciliata, the extract of Angelica sinensis and the extract of Uncaria catechu are mixed, and then 1100g of water and 700g of 1,3-propylene glycol are added and mixed. The mixture is treated with a low-temperature microfluidizer. The working temperature of the low-temperature microfluidizer is 10°C and the working pressure is 15000psi. The treatment is carried out three times to prepare a traditional Chinese medicine composition with soothing and repairing effects. The particle size of the traditional Chinese medicine composition is 56nm.

[0147] Example 11

[0148] The Chinese herbal composition with soothing and repairing effects of this embodiment includes: 1.12g of Bletilla striata extract, 1.12g of Elsholtzia ciliata extract, 1.4g of Angelica sinensis extract and 1.2g of Uncaria catechu extract.

[0149] The preparation method is as follows: according to the formula amount, the white ampelopsis extract, the stone holly extract, the angelica sinensis extract and the catechu uncaria extract are mixed, and then 1100g of water and 700g of 1,3-propylene glycol are added to mix, and a stirrer is used for 1h at a stirring speed of 500rpm to prepare a traditional Chinese medicine composition with soothing and repairing effects, wherein the particle size of the traditional Chinese medicine composition is 1225nm.

[0150] Comparative Example 1

[0151] The Chinese medicine composition of this comparative example comprises: 1.12 g of Bletilla striata extract and 1.12 g of Elsholtzia ciliata extract.

[0152] Comparative Example 2

[0153] The Chinese medicine composition of this comparative example comprises: 1.12 g of Bletilla striata extract and 1.12 g of Uncaria catechu extract.

[0154] Comparative Example 3

[0155] The Chinese medicine composition of this comparative example comprises: 112 g of Elsholtzia ciliata extract and 112 g of Angelica sinensis tricholoma extract.

[0156] Comparative Example 4

[0157] The Chinese medicine composition of this comparative example comprises: 1.12 g of Bletilla striata extract, 1.12 g of Angelica sinensis extract and 1.12 g of Uncaria catechu extract.

[0158] Comparative Example 5

[0159] The Chinese medicine composition of this comparative example comprises: 1.12g of Bletilla striata extract, 1.12g of Angelica sinensis extract, 1.12g of Uncaria catechu extract and 12g of Scutellaria baicalensis extract.

[0160] The preparation method of the scutellaria baicalensis extract is as follows: crushing the scutellaria baicalensis medicinal material, refluxing and extracting it with eight times the amount of 70wt% ethanol for 3 times, each time for 2 hours, combining the filtrates, and concentrating them into powder under reduced pressure to obtain the scutellaria baicalensis extract.

[0161] The preparation methods of the Chinese medicine compositions of Comparative Examples 1 to 5 are consistent with those of Examples 1 to 10.

[0162] In order to verify the efficacy and activity of the traditional Chinese medicine composition of the present application, the antioxidant properties, anti-inflammatory activity and stability of the traditional Chinese medicine compositions prepared in the above embodiments and comparative examples were verified, and the efficacy and activity and stability described in the traditional Chinese medicine compositions were verified by tests such as DPPH free radical scavenging test, inflammatory factor IL-6 inhibition, and inflammatory factor NO inhibition.

[0163] Test Example 1 Stability Test of Traditional Chinese Medicine Composition

[0164] The stability tests were performed on the samples of the traditional Chinese medicine compositions prepared in Examples 1 to 11 and Comparative Examples 1 to 5, and the physical state, color and odor of the samples were analyzed, tested and recorded. The samples were stored in sealed clean packaging materials and stored for 90 days under the following four conditions: high temperature (50°C), low temperature (-15°C), natural light (room temperature), and room temperature in the dark for 3 months. The storage environment temperature was 25°C ± 5°C, and the storage environment humidity was 50% RH ± 20%. The changes in the sample morphology (color, odor, state) were observed at 0 days, 30 days, 60 days, and 90 days, respectively. The data at 30 days, 60 days, and 90 days were compared with the data at 0 days to judge the stability of the traditional Chinese medicine formula. The results showed that the traditional Chinese medicine composition samples of Examples 1 to 11 were brown transparent liquids with a special odor. On day 0, the samples all met the standards (no precipitation in the material and no color change). On day 30, the sample of Example 11 had precipitation, while the other samples showed no obvious changes and all met the standards (no precipitation in the material and no color change). On days 60 and 90, the sample of Example 11 was found to have severe precipitation, while the other samples showed no obvious changes and all met the standards (no precipitation in the material and no color change). It can be seen that there is a significant difference in sample stability between Example 11 that has not been treated with low-temperature microfluidization and Examples 1-10 that have been treated with low-temperature microfluidization. The samples of the traditional Chinese medicine composition treated with low-temperature microfluidization in this application have good stability within 3 months and no abnormalities have occurred.

[0165] Test Example 2 Antioxidant Activity Test of Traditional Chinese Medicine Composition

[0166] The antioxidant activity of the Chinese medicine composition samples prepared in Examples 1 to 11 and Comparative Examples 1 to 5 was tested using the following method:

[0167] Accurately weigh 41.67 mg of DPPH reagent and dilute to a 100 mL brown volumetric flask. Take 5 mL of the DPPH reagent and dilute to a 50 mL brown volumetric flask with anhydrous ethanol to obtain a DPPH test solution (0.1 mmol / L). Pipette 1 mL of the DPPH test solution and add 0.5 mL of a 2 wt% concentration of any of the samples in Examples 1-11 and Comparative Examples 1-5. After thorough mixing, let the solution stand in the dark for 30 minutes. Measure the absorbance at 517 nm using anhydrous ethanol as a blank control and vitamin C as a control. Calculate the DPPH scavenging rate of each sample at a 2 wt% concentration according to the following formula (1).

[0168] (1)

[0169] Where: A s ——Absorbance of the reaction system of sample solution and DPPH; A r ——Absorbance of sample solution-anhydrous ethanol; A0——Absorbance of blank control-anhydrous ethanol.

[0170] The results obtained by the above test method are shown in Table 1.

[0171] Table 1: Antioxidant activity test results of Chinese herbal medicine compositions

[0172]

[0173] As can be seen from Table 1, compared with Comparative Examples 1 to 5, the Chinese medicine composition samples prepared in Examples 1 to 11 have a higher DPPH free radical scavenging rate, among which Examples 1-6 have the best scavenging rate, indicating that the scientifically formulated Chinese medicine composition has better antioxidant effect, can effectively alleviate the degradation of cell structure and function under the action of excessive free radicals generated after external stimulation, and has a good effect of promoting skin barrier repair.

[0174] Test Example 3 Hyaluronidase Activity Inhibition Test of Traditional Chinese Medicine Composition

[0175] The samples of the traditional Chinese medicine compositions prepared in Examples 1 to 11 and Comparative Examples 1 to 5 were subjected to a hyaluronidase activity inhibition test, and the test method was as follows:

[0176] The hyaluronidase inhibition method was used to investigate the in vitro anti-allergic efficacy of the Chinese medicine composition. 0.1 mL of CaCl2 solution (0.25 mmol / L) and 0.5 mL of hyaluronidase solution (100 U / mL) were reacted in a 37°C water bath for 20 min; 0.5 mL of each Chinese medicine composition sample was added and kept warm for 20 min; 0.5 mL of sodium hyaluronate solution (0.5 g / L) was added, reacted for 30 min, and then cooled for 5 min; 0.1 mL of sodium hydroxide solution (4 mol / L) and 0.5 mL of acetylacetone solution (3.5 mL of acetylacetone dissolved in 50 mL of 1.0 mol / L sodium carbonate solution) were added, and the mixture was boiled in a water bath for 15 min, then immediately transferred to an ice water bath for 5 min; 1 mL of Ehrlich reagent (0.8 g of p-dimethylaminobenzaldehyde dissolved in 15 mL of concentrated hydrochloric acid and 15 mL of anhydrous ethanol) was added dropwise and diluted with 3 mL of anhydrous ethanol. The mixture was allowed to stand at 25°C for 20 min for color development, and the absorbance was measured at 540 nm, with dipotassium glycyrrhizate as the control. The hyaluronidase inhibition rate was calculated according to the following formula (2):

[0177] (2)

[0178] In the formula: A is the absorbance of the control solution (acetic acid buffer solution is used instead of the sample solution); B is the absorbance of the control blank solution (acetic acid buffer solution is used instead of the sample solution and enzyme solution); C is the absorbance of the sample solution; D is the absorbance of the sample blank solution (acetic acid buffer solution is used instead of the enzyme solution).

[0179] The results obtained by the above test method are shown in Table 2.

[0180] Table 2: Hyaluronidase activity inhibition test results of traditional Chinese medicine compositions

[0181]

[0182] As can be seen from Table 2, the hyaluronidase inhibition performance of the traditional Chinese medicine compositions prepared in Examples 1 to 11 is higher than that of Comparative Examples 1 to 5, indicating that the traditional Chinese medicine compositions prepared in the present application have excellent anti-allergic efficacy and anti-inflammatory activity, among which the anti-allergic efficacy and anti-inflammatory activity of Examples 1 to 6 are better.

[0183] Test Example 4: Test on the inhibition of inflammatory factor NO by a Chinese herbal composition

[0184] The samples of the traditional Chinese medicine compositions prepared in Examples 1 to 11 and Comparative Examples 1 to 5 were tested for their inflammatory factor NO inhibitory activity. The testing method was as follows:

[0185] The concentration of the RAW 264.7 cell suspension was adjusted and added to a 96-well cell plate. The cells were cultured overnight at 37°C and 5% CO2 for detection. NMN was diluted to a non-cytotoxic concentration using a cell culture medium containing LPS and then added. The sample groups were prepared by adding the Chinese medicine composition samples of Examples 1 to 11 and Comparative Examples 1 to 5 to the above culture medium. The LPS-containing culture medium was used as the negative control (NC). The positive control group (PC) was added with a culture medium containing vitamin A and LPS. The cell culture medium without LPS was used as the blank control (BC). After adding the sample, the cells were cultured for 20-24 hours. After the reaction ended, the content of the inflammatory mediator NO in the supernatant was determined using Griess reagent. The calculation method of NO is shown in formula (3):

[0186] (3)

[0187] Where: OD sample ——Absorbance of sample group; OD PC ——Absorbance of the positive control group.

[0188] The results obtained by the above test method are shown in Table 3.

[0189] Table 3: Test on inhibition of inflammatory factor NO by Chinese herbal composition

[0190]

[0191] Test Example 5: Test on the inhibition of inflammatory factor IL-6 by a Chinese herbal composition

[0192] The samples of the traditional Chinese medicine compositions prepared in Examples 1 to 11 and Comparative Examples 1 to 5 were tested for their inflammatory factor IL-6 inhibitory activity, and the testing method was as follows:

[0193] The concentration of RAW264.7 cell suspension was adjusted and added to a 96-well cell plate. The cells were cultured overnight at 37°C and 5% CO2 for detection. The cells were diluted with LPS-containing cell culture medium to a non-cytotoxic concentration and then added. The sample groups were prepared by adding the Chinese medicine composition samples of Examples 1 to 11 and Comparative Examples 1 to 5 to the above culture medium. The LPS-containing culture medium was used as the negative control (MC), and the LPS-free cell culture medium was used as the blank control (BC). The positive control diluted with the LPS-containing cell culture medium was used as the positive control (PC). After adding the sample, the cells were cultured for 20h-24h. After the reaction was completed, the supernatant was taken and the IL-6 content was detected according to the operating procedures of the IL-6E1isa kit. The calculation method of IL-6 is shown in formula (4):

[0194] (4)

[0195] Where: T is the average value of inflammatory factor IL-6 in negative control group; NC is the average value of inflammatory factor IL-6 in experimental group.

[0196] The results obtained by the above test method are shown in Table 4.

[0197] Table 4: Test results of the Chinese herbal composition inhibiting the inflammatory factor IL-6

[0198]

[0199] It can be seen from Tables 3 and 4 that the Chinese medicine composition has a good inhibitory effect on the inflammatory factors IL-6 and NO. At the same time, compared with Comparative Examples 1 to 5, Examples 1 to 11 have more obvious inhibitory effects, indicating that the Chinese medicine composition after reasonable compounding has excellent anti-inflammatory properties.

[0200] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0201] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A Chinese medicine composition with soothing and repairing effects, characterized in that: The Chinese medicine composition comprises Bletilla striata, Elsholtzia ciliata, Angelica sinensis with long teeth and Uncaria catechu. The Bletilla striata comprises Bletilla striata medicinal materials and / or Bletilla striata extract, the Elsholtzia ciliata comprises Elsholtzia ciliata medicinal materials and / or Elsholtzia ciliata extract, the Angelica sinensis with long teeth medicinal materials and / or Angelica sinensis with long teeth extract, and the Uncaria catechu comprises Uncaria catechu medicinal materials and / or Uncaria catechu extract.

2. The Chinese medicine composition with soothing and repairing effects according to claim 1, characterized in that: The mass content of total polyphenols in the Bletilla striata is ≥8%; and / or the mass content of total flavonoids in the Elsholtzia ciliata is ≥5%; and / or the mass content of coumarin components in the Angelica sinensis is ≥3%; and / or the mass content of catechin components in the Uncaria catechu is ≥15%.

3. The Chinese medicine composition with soothing and repairing effects according to claim 1 or 2, characterized in that: The Chinese medicine composition also satisfies at least one of the following characteristics (1) to (6): (1) In parts by mass, the composition of the Chinese medicine composition includes 10-20 parts of Bletilla striata, 8-20 parts of Elsholtzia ciliata, 1-10 parts of Angelica sinensis and 1-10 parts of Uncaria catechu; (2) The Chinese medicine composition further comprises a solvent; (3) extracting the Bletilla striata medicinal material with an extraction solvent to prepare the Bletilla striata extract; (4) extracting the Radix Angelicae Sinensis medicinal material with an extraction solvent to prepare the Radix Angelicae Sinensise extract; (5) performing supercritical extraction on the Elsholtzia ciliata medicinal material to prepare the Elsholtzia ciliata extract; (6) The Uncaria rhynchophylla catechu extract is prepared by supercritical extraction of the Uncaria rhynchophylla catechu.

4. The Chinese medicine composition with soothing and repairing effects according to claim 3, characterized in that: The Chinese medicine composition further satisfies at least one of the following (1) to (10): (1) The solvent includes one or more of polyol and water; (2) The ratio of the total mass of the white ampelopsis, the scutellaria baicalensis, the angelica sinensis and the catechu Uncaria rhynchophylla to the mass of the solvent is (0.5-1.5): (50-70); (3) The extraction solvent includes one or more of water and an organic solvent; (4) The mass ratio of the white ampelopsis medicinal material and the Angelica sinensis medicinal material to the extraction solvent is 1: (10-20); (5) The pressure of supercritical extraction is 20MPa-40MPa; (6) The temperature of supercritical extraction is 40℃-50℃; (7) The separation pressure of supercritical extraction is 5MPa-10MPa; (8) The separation temperature of supercritical extraction is 30℃-70℃; (9) The solvent for supercritical extraction is one or more of water and organic solvent; (10) Supercritical extraction includes supercritical CO2 extraction.

5. The method for preparing the Chinese medicine composition with soothing and repairing effects according to any one of claims 1 to 4, characterized in that: The following steps are involved: The Chinese medicinal composition with soothing and repairing effects is prepared by mixing white ampelopsis, licorice root, angelica sinensis and Uncaria catechu.

6. The method for preparing the Chinese medicine composition with soothing and repairing effects according to claim 5, characterized in that: Microfluidization is used during the mixing process, the operating temperature of the microfluidization is 0° C.-70° C., and the operating pressure of the microfluidization is 1000 psi-30000 psi; and / or, a solvent is added during the mixing process.

7. A preparation for external use, characterized in that The ingredients of the external preparation include the traditional Chinese medicine composition with soothing and repairing efficacy according to any one of claims 1 to 4 or the traditional Chinese medicine composition with soothing and repairing efficacy prepared by the preparation method according to claim 5 or 6, and excipients.

8. The external preparation according to claim 7, characterized in that The mass addition amount of the Chinese medicine composition in the external preparation is 0.5%-10%; and / or, the excipients include one or more of fillers, diluents, lubricants, wetting agents and adhesives; and / or, the dosage form of the external preparation includes powder, ointment, pill, wine, tablet, aqueous solution, aerosol or papule.

9. A cosmetic, characterized in that The ingredients of the cosmetic include the traditional Chinese medicine composition with soothing and repairing effects according to any one of claims 1 to 4 or the traditional Chinese medicine composition with soothing and repairing effects prepared by the preparation method according to claim 5 or 6, and additives.

10. The cosmetic according to claim 9, characterized in that The cosmetic also satisfies at least one of the following characteristics (1) to (4): (1) The amount of the Chinese medicine composition added to the cosmetic is 0.5%-10% by mass; (2) The additives include one or more of a moisturizer, a surfactant, an antioxidant, and a stabilizer; (3) The cosmetic is in the form of a cream, liquid, emulsion or foam; (4) The cosmetics include essence, cream, mask or cleanser.

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