Extraction reagent and extraction method for functional components of natural plants

Abstract

The invention discloses an extraction reagent and an extraction method for functional components of natural plants. The method is applied to extraction of curcumin in turmeric, xanthophyll in marigold, linalool in lavender, rosmarinic acid in basil, ellagic acid in pomegranate, anthocyanin in lycium ruthenicum, isorhamnetin in sea-buckthorn, ginsenoside in ginseng and baicalin in scutellaria baicalensis. According to the method, the potential of DES in replacement of a traditional solvent can be visually displayed, and the method is suitable for courses of chemistry, environmental science or materials science and helps students to understand core concepts (such as recoverability and low-toxicity design) of green chemistry. Experiment cost is low, operation is safe, and the device is suitable for teaching laboratories.

Description

Technical Field

[0001] The invention belongs to the technical field of novel green solvents, and in particular relates to an extraction reagent and an extraction method for natural plant functional components. Background Art

[0002] With growing awareness of environmental protection and increased attention to pollution sources, the development of resource-saving and environmentally friendly green chemical processes is imperative. Biocatalysis is the large-scale use of microorganisms or enzymes as catalysts to produce chemicals, pharmaceuticals, energy, and materials. It boasts high process efficiency (low energy consumption and high atom economy), mild reactions (low energy consumption), and environmental friendliness (low pollution). This aligns with the "green chemistry" philosophy of efficient resource utilization and energy conservation and emission reduction, and is a key trend in the development of green chemistry and green chemical engineering. Existing solvent technologies are widely used in various fields, but they come with the following major drawbacks: 1. Environmental and health hazards, such as toxicity risks: Organic solvents such as benzene, toluene, and chloroform are neurotoxic or carcinogenic, posing a threat to worker health with long-term exposure. 2. Safety risks: Flammability and explosiveness: Low-flash-point solvents such as ethanol and acetone are prone to fire, requiring additional explosion-proof measures and increasing production costs. Corrosiveness: Strong acidic / alkaline solvents (such as concentrated sulfuric acid and sodium hydroxide solution) corrode equipment, shortening its service life. 3. Efficiency and Cost Issues: Recycling Difficulties: Separation of high-boiling-point solvents (such as DMF) consumes high energy, resulting in low recovery rates (less than 50% in some industries), increasing waste disposal costs. Limited Selectivity: Some reactions require specific solvents (such as non-polar solvents to dissolve polymers), limiting process flexibility. High Carbon Footprint: Traditional solvent production relies on fossil fuels. For example, each ton of acetone produced emits approximately 2.5 tons of CO₂. Supercritical Fluids: Supercritical CO₂ technology requires high-pressure equipment (typically >73 atm), increasing initial investment costs by 30%-50%. Ionic Liquids: Complex synthesis (some require multi-step reactions) and high prices (approximately $100-$1000 / kg) limit large-scale application. Water-based Solvents: Poor solubility for hydrophobic substances and high drying energy consumption (water has a heat of vaporization of 2260 kJ / kg). These issues include:

[0003] Deep eutectic solvents (DES) are an emerging green solvent technology. Their core concept is to form a low-melting-point liquid system by mixing hydrogen bond donors (such as urea and polyols) with hydrogen bond acceptors (such as choline chloride) in a specific molar ratio. Compared with traditional ionic liquids, DES offer significant advantages such as readily available raw materials, simple preparation (no purification required), and biodegradability. Their physicochemical properties can also be flexibly manipulated through molecular design. Their technological breakthrough lies in their unique solvation capacity: the strong hydrogen-bonding network within DES efficiently dissolves biomass components such as cellulose and lignin, demonstrating their potential as a replacement for toxic organic solvents in biorefining. Furthermore, their wide electrochemical window (>2V) and ionic conductivity make them suitable as novel electrolyte materials for metal electrodeposition and energy storage device development. In the pharmaceutical field, DES can serve as reaction media to accelerate drug synthesis and enhance the bioavailability of poorly soluble drugs by forming drug cocrystals. Key technological approaches focus on molecular engineering, using computer simulations to predict interactions between components and experimental screening to optimize solvent performance parameters (such as viscosity and polarity). Current research hotspots include the development of light / heat responsive intelligent DES systems and the construction of recyclable closed-loop solvent systems. This technology is expected to promote green transformation in the fields of chemical industry, energy, medicine, etc., and achieve the dual goals of "atom economy" and process intensification. In addition, deep eutectic solvents (DES), as the preferred alternative to ionic liquids, also have the characteristics of ionic liquids. DES composed of hydrogen bond donors (HBD) and hydrogen bond acceptors (HBA) have unique advantages over ionic liquids. DES can be easily prepared by mixing cheap HBD and HBA at a certain molar ratio and temperature without further purification, and a high atomic utilization rate of 100% will be achieved during the synthesis process. As a new generation of green organic solvents, DES has similar properties to ionic liquids (Ma et al. 2021), such as high stability, low volatility, low vapor pressure and adjustable polarity. DES is easy to prepare and inexpensive, making it both efficient and economical. Therefore, the DES method for lignin separation holds broad application prospects. DES is also easily recyclable, and its composition can be optimized to optimize its recyclability, further improving the process's economics. Furthermore, as a new green solvent, DES offers advantages such as non-toxicity, low volatility, and biodegradability, potentially reducing or eliminating the use and generation of substances harmful to the environment and human health during the design, development, and application of chemicals and their production processes. The emergence of DES has opened up new areas for enzyme catalysis. Enzymes, as the primary catalysts in biocatalysis, possess characteristics such as high efficiency, specificity, mildness, and tunable activity. Their catalytic performance is influenced by conditions such as the reaction medium, substrate properties, reaction temperature, and reaction pH. Microbial catalysts utilize intact microbial cells as catalysts, and the catalysis remains essentially an intracellular enzymatic reaction.Due to the protective effects of structures such as cell walls and membranes, enzymes in cells are in a relatively suitable environment, resulting in improved stability of microbial catalysts during reactions. Enzyme biocatalysis technology aligns with the "green chemistry" philosophy of efficient resource utilization and energy conservation and emission reduction, and is a key trend in the development of green chemistry and green chemical engineering. Non-aqueous biocatalysis offers broad prospects for biocatalytic reactions involving many water-insoluble substrates, opening up new research areas for the development of green chemistry. In recent years, a major research direction in non-aqueous biocatalysis has been the greening of reaction media. This concept represents a significant shift from a passive approach to a proactive approach to sustainable development. Traditional organic solvents are used to separate and extract natural bioactive substances, but they are prone to residues and environmental pollution, which is inconsistent with the principles of green chemistry. Therefore, it is crucial to find green and environmentally friendly alternatives to traditional organic solvents for extraction and separation. Currently, deep eutectic solvents are widely used in a variety of fields, including organic synthesis, materials chemistry, electrochemistry, and biocatalysis. The extraction and separation of raw materials or products is a crucial unit operation in the chemical industry. As a green solvent, DES has been widely used in the extraction of natural products. According to published literature, DESs have been used to extract flavonoids, phenolics, terpenes, saponins, anthocyanins, and other compounds from plant raw materials, achieving extraction yields exceeding those of traditional organic solvents. In addition to hydrophilic DESs, hydrophobic DESs have also been demonstrated to be useful for the extraction of hydrophobic natural active ingredients. As a novel green solvent medium, deep eutectic solvents possess unique solubility properties, and therefore hold great potential for the separation and extraction of functional plant components. DESs not only possess the excellent properties of traditional ionic liquids but also offer simple preparation, low cost, and environmental friendliness. Their applications in various fields, including environmental science, biology, catalysis, and chemistry, have been widely reported. DESs can be used in electrochemistry to study the electrochemical behavior between electrodes and solvents. Studies have used cyclic voltammetry and electrochemical impedance spectroscopy to evaluate the interfacial properties between platinum, gold, and glassy carbon electrodes and deep eutectic solvents based on choline chloride and glycerol. DESs are among the most promising green solvents in recent years, and their hydrophobicity and hydrophilicity can be modulated by modifying their inherent hydrogen bond donors and acceptors. DES can replace environmentally polluting, volatile organic solvents or expensive ionic liquids as the main medium for biocatalysis. At the same time, it has the characteristics of ionic liquids and can dissolve and disperse some substances that are difficult to dissolve or insoluble in water. The discovery of DES is more in line with the concept of green chemistry. Many DES can be recycled after extraction. Moreover, because DES is not easy to volatilize and has stable properties, it is also easy to operate and transport.Recent research has also revealed new applications for DES in analytical testing. DES has made significant contributions to the detection of some bioactive substances. Adding a certain amount of DES to the corresponding buffer can enhance the electrochemical detection signal of antioxidants such as quercetin (QR). The applications of DES in analytical chemistry are primarily reflected in the following areas: (a) extracting target analytes from complex matrices for quantitative determination using analytical instruments; (b) acting as a dispersant and stabilizer for materials, modifying silica for solid-phase extraction and chromatography, and as an adsorbent in chromatographic columns; and (c) as an additive to mobile phases in chromatography. DES has also played a significant role in nanotechnology and the preparation of biodiesel.

[0004] Invention content In view of this, the present invention aims to provide an extraction reagent and extraction method for natural plant functional ingredients to solve at least one technical problem in the background technology.

[0005] To achieve the above object, the technical solution created by the present invention is implemented as follows: The invention discloses an extraction reagent for natural plant active ingredients, comprising choline chloride, glucose and ethylene glycol.

[0006] Furthermore, the molar ratio of choline chloride, glucose and ethylene glycol is 1:1.5-2.5:1.

[0007] Furthermore, the molar ratio of choline chloride, glucose and ethylene glycol is 1:2:1.

[0008] The preparation method of the above-mentioned extraction reagent of natural plant functional ingredients comprises the following steps: mixing choline chloride and glucose in proportion, adding ethylene glycol in proportion, heating to 80-90° C., and stirring for 0.5-2 hours.

[0009] A method for preparing an extraction reagent for natural plant functional components is disclosed. The prepared extraction reagent for natural plant functional components is used in extracting functional components from natural plants.

[0010] It is used to extract rosmarinic acid from rosemary leaves, curcumin from turmeric, lutein from marigold, linalool from lavender, rosmarinic acid from basil, ellagic acid from pomegranate, anthocyanins from black wolfberry, isorhamnetin from sea buckthorn, ginsenosides from ginseng, and baicalin from scutellaria.

[0011] A method for extracting a reagent for extracting natural plant functional ingredients comprises the following steps: S1: crushing the natural plant to be extracted, and mixing the crushed natural plant with the extraction reagent of the natural plant active ingredients; S2: Stir for 20-60 min at 30-80°C; S2: Centrifuge to separate the solid residue and collect the supernatant to obtain the natural plant active ingredients.

[0012] Compared with the prior art, the extraction reagent and extraction method of the natural plant functional ingredients created by the present invention have the following advantages: 1. This application demonstrates the potential of DES in replacing traditional solvents and is suitable for use in chemistry, environmental science, or materials science courses, helping students understand core concepts of green chemistry (e.g., recyclability and low-toxicity design). The experiment is low-cost and safe, making it suitable for promotion in teaching laboratories.

[0013] 2. The design of DES in this application to meet the needs of different enzymatic reactions is still an unresolved issue. Ultrasonic-assisted deep eutectic solvents combined with biological enzymes, as a new technology for extracting bioactive ingredients, not only overcome the defects of traditional extraction solvents, but also expand the scope of biological enzymes in extraction applications, and extract water-soluble and fat-soluble components simultaneously in a green and efficient manner. Due to the good biocompatibility and reusability of deep eutectic solvents, the processing process is reduced and the production cost is reduced. This technology further expands the process range of deep eutectic solvents for extracting active substances, and provides a reference for the future development of green and efficient processes for extracting natural active substances.

[0014] 3. The extraction reagent of a natural plant active ingredient of the present application can be extracted efficiently: Due to the strong hydrogen bonding effect, the extraction efficiency of DES may be higher than that of ethanol or water, and the extraction time is shorter.

[0015] 2. The extraction reagent of a natural plant active ingredient in the present application is recyclable: the extraction efficiency of the recycled DES decreases by less than 10% after three cycles, reducing resource waste.

[0016] 3. The extraction reagent of a natural plant active ingredient of the present application is biodegradable: DES is significantly degraded by composting microorganisms within 4 weeks, while traditional solvents have a long residual time.

[0017] 4. The extraction reagent of a natural plant active ingredient of the present application has low toxicity: the germination rate of seeds treated with DES is close to that of the control group, while the germination of the seeds treated with traditional solvents is inhibited. DETAILED DESCRIPTION

[0018] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other.

[0019] The present invention will be described in detail below with reference to the embodiments.

[0020] Example 1: 13.96 of choline chloride and 18.02 of glucose were mixed in proportion, 12.41 g of ethylene glycol was added in proportion, and the mixture was heated to 80° C. and stirred for 0.5 hour to prepare DES as a transparent and uniform liquid.

[0021] Ingredients: Dried rosemary leaves (rosmarinic acid).

[0022] 1. Mix 1 g of crushed rosemary leaves with 10 mL of DES.

[0023] 2. Stir in a 60℃ water bath for 20 minutes.

[0024] 3. Centrifuge to separate the solid residue and collect the supernatant. The centrifugation speed is 4500 / min and the centrifugation time is 15 minutes.

[0025] 4. Determine the rosmarinic acid content in the extract by UV spectrophotometry or HPLC. Extract the rosmarinic acid with an antioxidant. The extraction rate of rosmarinic acid is 93.45%.

[0026] Example 2 Target ingredient: Curcumin 1. Mix the crushed turmeric with the DES prepared in Example 1, with the material-liquid ratio of the crushed turmeric to the DES being 1:15 (g / mL); 2. Stir in a 65°C water bath for 30 minutes. Add 0.2% Tween-80 to improve the solubility of curcumin. And with the assistance of microwave ultrasound (45kHz, 250W), the extraction time is shortened; 3. Centrifuge to separate the solid residue and collect the supernatant. The centrifuge speed is 4500 / min and the centrifugation time is 15 minutes.

[0027] 4. Determine the content of curcumin in the extract and extract curcumin with antioxidants. The extraction rate of curcumin is 94.45%.

[0028] Example 3 Target ingredient: Lutein 1. Crush marigold and mix with DES in Example 1. The material-liquid ratio of crushed marigold to DES is 1:2.

[0029] 2. Stir in a 30°C water bath for 55 minutes, adjust the pH to 4.0–5.5, and add a small amount of ethanol (7% v / v) to prevent lipid oxidation; use ultrasonic microwave assistance (650W, 2 minutes) to improve efficiency.

[0030] 3. Centrifuge to separate the solid residue and collect the supernatant. The centrifugation speed is 4500 / min and the centrifugation time is 15 minutes.

[0031] 4. Determine the lutein content in the extract and extract the lutein with antioxidants. The extraction rate of lutein is 93.42%.

[0032] Example 4 Target ingredient: Linalool 1. Mix crushed lavender with the DES prepared in Example 1, with the material-liquid ratio of crushed lavender to DES being 1:9 (g / mL); 2. Stir in a 55°C water bath for 23 minutes.

[0033] 3. Centrifuge to separate the solid residue and collect the supernatant. The centrifuge speed is 4500 / min and the centrifugation time is 15 minutes.

[0034] 4. Determine the linalool content in the extract and extract linalool with antioxidants. The extraction rate of linalool is 94.32%.

[0035] Example 5 Target ingredient: Rosmarinic acid 1. Mix crushed basil with the DES prepared in Example 1, with the material-liquid ratio of crushed basil to DES being 1:9 (g / mL); 2. Stir in a 75°C water bath for 30 minutes, add 0.04% ascorbic acid to prevent oxidative loss, and release intracellular components with cellulose plum and ultrasonic microwave assistance (400w for 2 minutes); 3. Centrifuge to separate the solid residue and collect the supernatant at 4500 rpm for 15 minutes.

[0036] 4. Determine the content of rosmarinic acid in the extract and extract rosmarinic acid with antioxidants. The extraction rate of rosmarinic acid is 93.50%.

[0037] Example 6 Target ingredient: Ellagic acid 1. Pomegranate crushed fruit was mixed with the DES prepared in Example 1, with the material-liquid ratio of pomegranate crushed fruit to DES being 1:13 (g / mL); 2. Stir in a 75°C water bath for 50 minutes and add 0.1% chitosan to flocculate impurities; 3. Centrifuge to separate the solid residue and collect the supernatant. Centrifuge at 4500 rpm for 15 minutes.

[0038] 4. Determine the content of ellagic acid in the extract. Extract ellagic acid with antioxidants. The extraction rate of ellagic acid is 93.56%.

[0039] Example 7 Target ingredient: anthocyanin 1. Crush the black wolfberry and mix it with the DES in Example 1. The material-liquid ratio of crushed black wolfberry to DES is 1:2 (g / mL).

[0040] 2. Stir in a 65°C water bath for 40 minutes and adjust the pH to 2.8–3.0; 3. Centrifuge to separate the solid residue and collect the supernatant; centrifuge at 4500 rpm for 15 minutes.

[0041] 4. Determine the anthocyanin content in the extract and extract the anthocyanin with antioxidants. The extraction rate of anthocyanin is 93.40%.

[0042] Example 8 Target ingredient: Isorhamnetin 1. Mix the crushed seabuckthorn and the DES in Example 1, with the material-liquid ratio of crushed seabuckthorn to DES being 1:25 (g / mL); 2. Stir in a 50°C water bath for 40 minutes and adjust the pH to 2.8–3.0; 3. Centrifuge to separate the solid residue and collect the supernatant. Centrifuge at 4500 rpm for 15 minutes.

[0043] 4. Determine the isorhamnetin content in the extract and extract isorhamnetin with antioxidants. The extraction rate of isorhamnetin is 93.50%.

[0044] Example 9 Target ingredient: Ginsenosides (triterpenoids, regulate oxidative stress pathways) 1. Grind ginseng and mix it with the DES prepared in Example 1. The material-liquid ratio of ginseng to DES is 1:2 (g / mL).

[0045] 2. Stir in a 75°C water bath for 85 minutes, adjust the pH to 5.0–6.5, and disrupt the cell membrane using microwave-assisted methods (850W, 5 minutes). 3. Centrifuge to separate the solid residue and collect the supernatant; 4. Determine the content of ginsenosides in the extract. The antioxidant extracts ginsenosides. The extraction rate of ginsenosides is 90.2%.

[0046] Example 10 Target ingredient: Baicalin (flavonoid glycoside, anti-inflammatory and antioxidant) The radix scutellariae was ground and mixed with the DES prepared in Example 1, with the solid-liquid ratio of radix scutellariae to DES being 1:2 (g / mL); 2. Stir in a 60°C water bath for 40 minutes and add 0.05% sodium hydroxide to improve the solubility of baicalin; use ultrasound (25 kHz, 300 W) to disrupt the covalent bond binding to the pectinase system; 3. Centrifuge to separate the solid residue and collect the supernatant; the centrifugal speed is 4500 / min and the centrifugal time is 15 minutes 4. Determine the content of baicalin in the extract and extract baicalin with antioxidants. The extraction rate of baicalin is 91.04%.

[0047] Example 11 DES solution recovery method: 1. Remove water and volatile components from the extracted DES solution by vacuum distillation (60°C, 0.1 MPa). 2. Add a small amount of deionized water (if solute separation is required) and recover the DES by liquid-liquid separation. 3. Verify recovery efficiency: Repeat the same extraction experiment three times using the recovered DES, measuring the extraction efficiency each time. 4. Biodegradability Test Method: Mix discarded DES (1 mL) with compost (simulating a natural environment) and a control with a traditional solvent (e.g., ethyl acetate). Observation: Measure the DES residue in the compost weekly (via TOC analysis or microbial activity assay) to compare degradation rates.

[0048] Toxicity comparison experiment Plant seed germination experiment: Pea seeds were treated with a DES aqueous solution (1:10 dilution) and a traditional solvent. Seed germination rate and root growth were observed over 7 days, and toxicity was compared. Expected results and environmental performance were demonstrated.

[0049] Efficient Extraction: Due to strong hydrogen bonding, DES has the potential to achieve higher extraction efficiency than ethanol or water, and in a shorter extraction time. Recyclability: The extraction efficiency of recycled DES decreases by less than 10% after three cycles, reducing resource waste. Biodegradability: DES is significantly degraded by composting microorganisms within four weeks, while traditional solvents have a longer residual lifespan. Low Toxicity: The germination rate of seeds treated with DES is close to that of the control, while germination is inhibited in the traditional solvent treatment.

[0050] The green chemistry principles of this application encompass atom economy: DES preparation requires no complex synthesis, resulting in high raw material utilization; reduction of hazardous substances: avoiding the use of volatile organic compounds (VOCs); energy efficiency: low extraction temperature (60°C vs. 80°C+ for traditional Soxhlet extraction); and renewable raw materials: choline chloride and urea can be derived from biomass or industrial byproducts. This experiment visually demonstrates the potential of DES as a replacement for traditional solvents and is suitable for students in chemistry, environmental science, or materials science courses, helping them understand core green chemistry concepts (such as recyclability and low-toxicity design). The experiment is low-cost and safe, making it suitable for implementation in teaching laboratories.

[0051] This application develops non-traditional hydrogen bond donor / acceptor combinations to break through the limitations of the traditional choline chloride / urea system; introduces bifunctional or multifunctional components (such as bio-based molecules with both catalytic and solvating capabilities) to achieve "solvent-catalyst" integration.

[0052] The high-efficiency and low-energy preparation process proposes green synthesis methods such as microwave assistance and mechanochemistry to shorten the reaction time to minutes (traditionally it takes several hours); establishes an in-situ solvent regeneration process to achieve continuous operation of reaction-separation-solvent recovery.

[0053] Directed functional regulation technology achieves precise adaptation of solvent polarity, viscosity, and solubility through dynamic hydrogen bond network regulation (such as increasing the solubility of poorly soluble drugs by more than 10 times); develops light / thermal / electrically responsive intelligent DES to achieve dynamic switching of solvent properties through external stimuli.

[0054] Closed-loop regeneration and recycling systems establish resource utilization pathways for solvent degradation products (such as conversion into fertilizers or chemical precursors).

[0055] Objects of protection: DES systems modified with light / heat-sensitive groups (such as azobenzene derivative doping) and their physical property mutation thresholds under triggering conditions (such as the wavelength / temperature required for a 50% decrease in viscosity).

[0056] Comparative Example 1 The difference from Example 1 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of rosmarinic acid is 90.1%.

[0057] Comparative Example 2 The difference from Example 1 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of rosmarinic acid is 89.5%.

[0058] Comparative Example 3 The difference from Example 1 is that no glucose is added, and the molar ratio of choline chloride to ethylene glycol is 1:1. The extraction rate of rosmarinic acid is 88.2%.

[0059] Comparative Example 4 The difference from Example 1 is that ethylene glycol is not added, the molar ratio of choline chloride to glucose is 1:2, and the extraction rate of rosmarinic acid is 85.5%.

[0060] Comparative Example 5 The difference from Example 2 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of curcumin is 90.2%.

[0061] Comparative Example 6 The difference from Example 2 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2: 1, and the extraction rate of curcumin is 89.5%.

[0062] Comparative Example 7 The difference from Example 2 is that no glucose is added, and the molar ratio of choline chloride to ethylene glycol is 1:1. The extraction rate of curcumin is 85.0%.

[0063] Comparative Example 8 The difference from Example 2 is that ethylene glycol is not added, and the molar ratio of choline chloride to glucose is 1:2. The extraction rate of curcumin is 82.2%.

[0064] Comparative Example 9 The difference from Example 3 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The lutein extraction rate is 80.2%.

[0065] Comparative Example 10 The difference from Example 3 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the lutein extraction rate is 79.85%.

[0066] Comparative Example 11 The difference from Example 3 is that no glucose was added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The lutein extraction rate was 78.70%.

[0067] Comparative Example 12 The difference from Example 3 is that ethylene glycol is not added, the molar ratio of choline chloride to glucose is 1:2, and the lutein extraction rate is 75.50%.

[0068] Comparative Example 13 The difference from Example 4 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of linalool is 92.30%.

[0069] Comparative Example 14 The difference from Example 4 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of linalool is 91.52%.

[0070] Comparative Example 15 The difference from Example 4 is that no glucose is added, and the molar ratio of choline chloride to ethylene glycol is 1:1. The extraction rate of linalool is 90.00%.

[0071] Comparative Example 16 The difference from Example 4 is that ethylene glycol is not added, the molar ratio of choline chloride to glucose is 1:2, and the extraction rate of linalool is 88.50%.

[0072] Comparative Example 17 The difference from Example 5 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of rosmarinic acid is 92.52%.

[0073] Comparative Example 18 The difference from Example 5 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of rosmarinic acid is 90.65%.

[0074] Comparative Example 19 The difference from Example 5 is that no glucose was added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The extraction rate of rosmarinic acid was 89.50%.

[0075] Comparative Example 20 The difference from Example 5 is that ethylene glycol is not added, and the molar ratio of choline chloride to glucose is 1:2. The extraction rate of rosmarinic acid is 88.20%.

[0076] Comparative Example 21 The difference from Example 6 is that the molar ratio of choline chloride, glucose, and ethylene glycol is 1:1:1. The extraction rate of ellagic acid is 92.10%.

[0077] Comparative Example 22 The difference from Example 6 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of ellagic acid is 91.65%.

[0078] Comparative Example 23 The difference from Example 6 is that no glucose was added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The ellagic acid extraction rate was 90.4%.

[0079] Comparative Example 24 The difference from Example 6 is that ethylene glycol was not added, the molar ratio of choline chloride to glucose was 1:2, and the extraction rate of ellagic acid was 89.2%.

[0080] Comparative Example 25 The difference from Example 7 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of anthocyanin is 92.50%.

[0081] Comparative Example 26 The difference from Example 7 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2: 1, and the extraction rate of anthocyanins is 91.65%.

[0082] Comparative Example 27 The difference from Example 7 is that no glucose was added, the molar ratio of choline chloride to ethylene glycol was 1:1, and the anthocyanin extraction rate was 90.50%.

[0083] Comparative Example 28 The difference from Example 7 is that ethylene glycol is not added, and the molar ratio of choline chloride to glucose is 1:2. The extraction rate of anthocyanin is 89.50%.

[0084] Comparative Example 29 The difference from Example 8 is that the molar ratio of choline chloride, glucose, and ethylene glycol is 1:1:1. The extraction rate of isorhamnetin is 92.52%.

[0085] Comparative Example 30 The difference from Example 8 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of isorhamnetin is 91.56%.

[0086] Comparative Example 31 The difference from Example 8 is that no glucose was added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The isorhamnetin yield was 90.40%, and the extraction rate was 90.40%.

[0087] Comparative Example 32 The difference from Example 8 is that ethylene glycol was not added, and the molar ratio of choline chloride to glucose was 1:2. The extraction rate of isorhamnetin was 89.30%.

[0088] Comparative Example 33 The difference from Example 9 is that the molar ratio of choline chloride, glucose, and ethylene glycol is 1:1:1. The extraction rate of ginsenosides is 91.60%.

[0089] Comparative Example 34 The difference from Example 9 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of ginsenosides is 90.20%.

[0090] Comparative Example 35 The difference from Example 9 is that glucose was not added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The ginsenoside extraction rate was 88.90%.

[0091] Comparative Example 36 The difference from Example 9 is that ethylene glycol was not added, and the molar ratio of choline chloride to glucose was 1:2. The extraction rate of ginsenosides was 87.40%.

[0092] Comparative Example 37 The difference from Example 10 is that the molar ratio of choline chloride, glucose and ethylene glycol is 1:1:1. The extraction rate of baicalin is 91.40%.

[0093] Comparative Example 38 The difference from Example 10 is that choline chloride is not added, the molar ratio of glucose to ethylene glycol is 2:1, and the extraction rate of baicalin is 90.60%.

[0094] Comparative Example 39 The difference from Example 10 is that no glucose was added, and the molar ratio of choline chloride to ethylene glycol was 1:1. The extraction rate of baicalin was 89.50%.

[0095] Comparative Example 40 The difference from Example 10 is that ethylene glycol is not added, and the molar ratio of choline chloride to glucose is 1:2. The extraction rate of baicalin is 84.62%.

[0096] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A natural plant functional ingredient extraction reagent, characterized in that: Includes choline chloride, dextrose, and ethylene glycol.

2. The extraction reagent of a natural plant functional component according to claim 1, characterized in that: The molar ratio of choline chloride, glucose and ethylene glycol is 1:1.5-2.5:

1.

3. The extraction reagent of a natural plant functional component according to claim 1, characterized in that: The molar ratio of choline chloride, glucose and ethylene glycol is 1:2:

1.

4. A method for preparing an extraction reagent for a natural plant functional component according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: mixing choline chloride and glucose in proportion, adding ethylene glycol in proportion, heating to 80-90° C., and stirring for 0.5-2 hours.

5. The extraction reagent of natural plant functional components prepared by the preparation method of the extraction reagent of natural plant functional components according to claim 4 is used in the extraction of functional components from natural plants.

6. The use according to claim 5, characterized in that: It is used to extract rosmarinic acid from rosemary leaves, curcumin from turmeric, lutein from marigold, linalool from lavender, rosmarinic acid from basil, ellagic acid from pomegranate, anthocyanins from black wolfberry, isorhamnetin from sea buckthorn, ginsenosides from ginseng, and baicalin from scutellaria.

7. The method for extracting a natural plant functional component extracting agent according to any one of claims 1 to 2, characterized in that: The steps include: S1: crushing the natural plant to be extracted, and mixing the crushed natural plant with the extraction reagent of the natural plant active ingredients; S2: Stir for 20-60 min at 30-80°C; S2: Centrifuge to separate the solid residue and collect the supernatant to obtain the natural plant active ingredients.