Tea tree oil microemulsion inclusion as well as preparation method and application thereof in anti-acne patch

Abstract

The invention provides a tea tree oil microemulsion inclusion. The tea tree oil microemulsion inclusion comprises the following components in percentage by mass: 5-15% of tea tree oil, 3-8% of an emulsifier, 1-5% of a stabilizer, 2-10% of a water-soluble polymer material and the balance of deionized water, the tea tree oil is extracted through a supercritical CO2 extraction method, and the average particle size of the tea tree oil microemulsion inclusion is 50-200 nm. The preparation method comprises the following steps: carrying out supercritical CO2 extraction on the tea tree oil; performing micro-jet emulsification; the tea tree oil microemulsion inclusion is obtained. The tea tree oil microemulsion inclusion is used for preparing the anti-acne patch, and the anti-acne patch is used for treating acne. The tea tree oil micro-emulsion system with high stability and low irritation is constructed by integrating a supercritical CO2 extraction technology and a micro-jet emulsification technology and combining the emulsifier, the stabilizer and the functional components in a specific proportion, and the tea tree oil micro-emulsion system can be applied to anti-acne patch products.

Description

Technical Field

[0001] The invention belongs to the technical field of tea tree oil skin care related products, and particularly relates to a tea tree oil microemulsion inclusion body, a preparation method thereof, and application in an anti-acne patch. Background Art

[0002] Tea tree oil, a natural essential oil extracted from the leaves and branches of Melaleuca alternifolia, is widely used in acne treatments for its antibacterial and anti-inflammatory properties. Its core active ingredient, 4-terpineol, effectively inhibits Propionibacterium acnes, while components like eucalyptol soothe redness and swelling.

[0003] Tea tree oil faces multiple challenges in practical application: First, as an oily substance, its solubility in water-based formulas is extremely low, and direct addition can easily lead to stratification or precipitation. Second, its high volatility makes it difficult for the active ingredient to remain in the skin for long periods of time, affecting the long-term effectiveness of its effects. Furthermore, untreated tea tree oil is highly irritating to the skin, and some users with sensitive skin may experience burning or allergic reactions. These issues limit its direct application in patch products. Existing anti-acne patches mostly rely on physical adsorption of pus or the addition of low-concentration salicylic acid, which cannot achieve the efficient penetration and sustained release of tea tree oil.

[0004] Traditional hydrocolloid dressings are based on sodium carboxymethylcellulose or polyvinylpyrrolidone, and they primarily assist in acne recovery by absorbing secretions and isolating external contaminants. However, these materials generally have insufficient loading capacity for oily components. Experimental data show that the loading capacity of conventional hydrocolloids for tea tree oil is usually less than 0.3 mg / cm 2 , and over time, "oil seepage" will occur, causing the patch to lose viscosity or even become ineffective. To solve this problem, existing technologies attempt to pre-emulsify tea tree oil and then mix it into a hydrocolloid. However, the emulsion prepared by traditional emulsification processes such as mechanical stirring or high-pressure homogenization has a large particle size, generally in the range of 500-1000nm, with insufficient transdermal absorption efficiency and poor stability. After two weeks of storage, the particle size is prone to growth or demulsification. On the other hand, some studies use liposomes or cyclodextrin to encapsulate tea tree oil. Although this can improve solubility, the encapsulation rate is often less than 60%, and the process is complex and costly, making it difficult to achieve industrial production.

[0005] In the tea tree oil extraction process, supercritical CO2 extraction technology has gradually replaced traditional distillation due to its advantages such as high selectivity and no solvent residue. However, existing patents mostly focus on the optimization of a single extraction process, adjusting the pressure or temperature to increase the yield, but have not solved the problem of connecting the extracted tea tree oil with the subsequent formulation process. Some solutions directly perform conventional emulsification after extraction, resulting in oxidation loss of active ingredients during multiple processing; others stabilize the emulsion by adding a large amount of surfactants, which increases the risk of skin irritation. In addition, the formula design of existing anti-acne patches lacks consideration of the skin microenvironment. If the pH value of the hydrocolloid does not match the natural pH of the skin, it may damage the barrier function and aggravate the inflammatory response.

[0006] Based on the above pain points, it is necessary to design a tea tree oil microemulsion inclusion body and its preparation method and application in anti-acne patches. Summary of the Invention

[0007] In order to overcome the defects in the prior art, a tea tree oil microemulsion inclusion body and a preparation method thereof and application in an anti-acne patch are provided.

[0008] In order to achieve the above object, the present invention provides the following technical solutions: A tea tree oil microemulsion inclusion body, comprising the following components in percentage by mass: 5-15% tea tree oil, 3-8% emulsifier, 1-5% stabilizer, 2-10% water-soluble polymer material, and the balance deionized water; The tea tree oil is extracted by a supercritical CO2 extraction method, and the average particle size of the tea tree oil microemulsion inclusions is 50-200nm.

[0009] The emulsifier is a mixture of polyglycerol-10 laurate and Tween-80, and the mass ratio of the emulsifier to polyglycerol-10 laurate and Tween-80 is 1-3:1.

[0010] The stabilizer is a mixture of hydroxypropyl methylcellulose and sodium alginate, the mass ratio of the hydroxypropyl methylcellulose to the sodium alginate is 2:1-4:1, and the viscosity of the hydroxypropyl methylcellulose is 4000-6000 mPa·s.

[0011] The water-soluble polymer material is sodium hyaluronate or carboxymethyl chitosan, and the deacetylation degree of the carboxymethyl chitosan is ≥85%.

[0012] The tea tree oil microemulsion inclusion body further comprises functional components, which include: 0.1-0.3% dipotassium glycyrrhizate, 0.2-0.6% niacinamide, and 0.2-0.4% Centella asiatica extract.

[0013] A method for preparing tea tree oil microemulsion inclusions, the method comprising the following steps: (1) Supercritical CO2 extraction of tea tree oil: crush the branches and leaves of Melaleuca alternifolia and put them into the extraction kettle, control the CO2 flow rate to 20-40L / h, extraction pressure to 25-35MPa, temperature to 40-50℃, extraction time to 2-4h, and obtain tea tree oil; (2) Microfluidization: The tea tree oil obtained in step (1) is mixed with an emulsifier, a stabilizer, and a water-soluble polymer material in the mass percentages according to claim 1, deionized water is added, and the mixture is circulated in a microfluidizer at a pressure of 120-180 MPa for 3-6 times to obtain tea tree oil microemulsion inclusions with an average particle size of 50-200 nm.

[0014] In step (1), the supercritical CO2 extraction process further adds entrainer ethanol, the volume ratio of the ethanol to CO2 is 1:10-1:5, and the purity of the ethanol is ≥99.5%.

[0015] The mixing process of step (2) also includes adding functional components: dipotassium glycyrrhizate 0.1-0.3%, niacinamide 0.2-0.6%, and Centella asiatica extract 0.2-0.4%; The order of addition during the mixing process is: first add the emulsifier and premix with tea tree oil, then add the stabilizer and deionized water, and finally add the water-soluble polymer material and functional components; The interactive cavity of the microfluidizer has an aperture of 75-150 μm, is made of diamond, has an emulsification temperature of 10-25° C., and takes 30-60 minutes.

[0016] After the microfluidization emulsification in step (2), cross-linking curing and freeze-drying are further included in sequence. The specific steps are: adding 0.1-0.5% polyethylene glycol diacrylate to the microemulsion suspension, curing for 10-20 minutes under ultraviolet light with a wavelength of 365nm; freeze-drying the tea tree oil microemulsion inclusions after cross-linking and curing, and the specific parameters are: pre-freezing temperature of -40°C to -50°C, pre-freezing time of 4-6 hours, and sublimation drying at a vacuum degree of ≤10Pa for 24-48 hours.

[0017] The invention discloses an application of a tea tree oil microemulsion inclusion body in an anti-acne patch. The tea tree oil microemulsion inclusion body is used to prepare the anti-acne patch, and the anti-acne patch is used for treating acne.

[0018] Compared with the prior art, the advantages and beneficial effects of the present invention are: 1. This application integrates supercritical CO2 extraction technology and microfluidization emulsification process, combined with specific proportions of emulsifiers, stabilizers and functional components, to construct a highly stable, low-irritation tea tree oil microemulsion system that can be used in anti-acne patch products.

[0019] 2. The average particle size of the tea tree oil microemulsion inclusions in this application is controlled within the range of 50-200nm, significantly smaller than the 500nm or larger particles used in traditional emulsification processes. This nanoscale particle size not only improves transdermal efficiency, increasing the skin penetration of the active ingredient 4-terpineol to more than twice that of conventional emulsions, but also maintains the long-term stability of the system through an electrostatic stabilization mechanism, overcoming the drawback of traditional emulsions that demulsify after two weeks of storage.

[0020] 3. This application uses a composite emulsifier of polyglycerol-10 laurate and Tween-80, with a mass ratio of 1-3:1, which reduces the risk of irritation while ensuring a high encapsulation rate. The hydrophobic chain of polyglycerol-10 laurate has a strong affinity with tea tree oil molecules, while Tween-80 prevents particle aggregation through steric hindrance. The two work together to stabilize the encapsulation rate at over 92%, far exceeding the level of liposome or cyclodextrin encapsulation processes. This composite emulsifier system reduces the amount of a single surfactant used, reduces the skin irritation of the final product, and significantly improves its applicability to sensitive skin.

[0021] 4. The introduction of water-soluble polymers such as sodium hyaluronate and carboxymethyl chitosan creates a dual stabilization mechanism. Sodium hyaluronate encapsulates the oil droplets through a hydrogen bond network, while carboxymethyl chitosan utilizes its cationic properties to adsorb onto the microemulsion surface, enhancing the system's shear resistance. Furthermore, carboxymethyl chitosan and 4-terpineol in tea tree oil produce a synergistic antibacterial effect, significantly improving the inhibition rate against Propionibacterium acnes compared to single components. The resulting moisturizing film can alleviate dry skin discomfort.

[0022] 5. In the preparation process of this application, ethanol is added as an entrainer during the supercritical CO2 extraction stage. The ethanol-CO2 mixed fluid selectively extracts high-purity 4-terpineol, improving yield compared to traditional distillation methods while avoiding thermal decomposition of eucalyptol caused by high temperatures. Microfluidization is performed directly after extraction, and cyclic crushing under high pressure achieves nanoscale dispersion of the oil phase, shortening the process time and reducing the oxidation loss rate of the active ingredient to less than 3%.

[0023] 6. This application further optimizes product morphology by combining cross-linking and freeze-drying processes. The addition of polyethylene glycol diacrylate and UV curing form a three-dimensional cross-linked network on the microemulsion surface, reducing the evaporation rate of the tea tree oil. The subsequent freeze-drying step removes moisture under vacuum conditions, resulting in a solid microemulsion powder with a fluffy, porous structure that facilitates long-term storage and transportation.

[0024] 7. The synergistic effect of the functional components of this application, dipotassium glycyrrhizate, niacinamide, and Centella asiatica extract, expands the product's efficacy. Dipotassium glycyrrhizate inhibits inflammation, niacinamide regulates sebum secretion, and asiaticoside promotes epidermal cell proliferation. These three, combined with the antibacterial properties of tea tree oil, shorten the healing period for moderate to severe acne and reduce the incidence of post-treatment erythema.

[0025] 8. The meticulous control of process parameters in this application is also unique. The high pressure and low temperature conditions employed in the supercritical extraction stage ensure the extraction rate of 4-terpineol while increasing the retention rate of the heat-sensitive component α-terpinene. The low-temperature operation of 15-20°C in the microfluidization emulsification process and the diamond cavity design effectively avoid localized high temperatures caused by high-pressure shearing, ensuring that the degradation rate of the active ingredient is less than 2%. DETAILED DESCRIPTION

[0026] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0027] In this application, the sources of various raw materials are briefly described as follows: Polyglyceryl-10 laurate was purchased from Shanghai Xinshou Auxiliary Co., Ltd. (INCI name: Polyglyceryl-10 Laurate). Hydroxypropyl methylcellulose (HPMC) was IFF's METHOCEL™ K4M. Sodium hyaluronate was a high molecular weight product from Shiseido, Japan. Carboxymethyl chitosan was purchased from Zhejiang Jinke Pharmaceuticals. Tween-80 (polysorbate 80) was purchased from Shijiazhuang Ruisheng Pharmaceuticals Co., Ltd. Sodium alginate was purchased from Zhengzhou Chaofan Chemical Co., Ltd. (Chaofan Brown Sodium Alginate). Polyethylene glycol diacrylate (PEGDA) was purchased from Advanced Biomatrix, USA.

[0028] A tea tree oil microemulsion inclusion body, comprising the following components in percentage by mass: 5-15% tea tree oil, 3-8% emulsifier, 1-5% stabilizer, 2-10% water-soluble polymer material, and the balance deionized water; The tea tree oil is extracted by a supercritical CO2 extraction method, and the average particle size of the tea tree oil microemulsion inclusions is 50-200nm.

[0029] The emulsifier is a mixture of polyglycerol-10 laurate and Tween-80, and the mass ratio of the emulsifier to polyglycerol-10 laurate and Tween-80 is 1-3:1.

[0030] The stabilizer is a mixture of hydroxypropyl methylcellulose and sodium alginate, the mass ratio of the hydroxypropyl methylcellulose to the sodium alginate is 2:1-4:1, and the viscosity of the hydroxypropyl methylcellulose is 4000-6000 mPa·s.

[0031] The water-soluble polymer material is sodium hyaluronate or carboxymethyl chitosan, and the deacetylation degree of the carboxymethyl chitosan is ≥85%.

[0032] The tea tree oil microemulsion inclusion body further comprises functional components, which include: 0.1-0.3% dipotassium glycyrrhizate, 0.2-0.6% niacinamide, and 0.2-0.4% Centella asiatica extract.

[0033] A method for preparing tea tree oil microemulsion inclusions, the method comprising the following steps: (1) Supercritical CO2 extraction of tea tree oil: crush the branches and leaves of Melaleuca alternifolia and put them into the extraction kettle, control the CO2 flow rate to 20-40L / h, extraction pressure to 25-35MPa, temperature to 40-50℃, extraction time to 2-4h, and obtain tea tree oil; (2) Microfluidization: The tea tree oil obtained in step (1) is mixed with an emulsifier, a stabilizer, and a water-soluble polymer material in the mass percentages according to claim 1, deionized water is added, and the mixture is circulated in a microfluidizer at a pressure of 120-180 MPa for 3-6 times to obtain tea tree oil microemulsion inclusions with an average particle size of 50-200 nm.

[0034] In step (1), the supercritical CO2 extraction process further adds entrainer ethanol, the volume ratio of the ethanol to CO2 is 1:10-1:5, and the purity of the ethanol is ≥99.5%.

[0035] The mixing process of step (2) also includes adding functional components: dipotassium glycyrrhizate 0.1-0.3%, niacinamide 0.2-0.6%, and Centella asiatica extract 0.2-0.4%; The order of addition during the mixing process is: first add the emulsifier and premix with tea tree oil, then add the stabilizer and deionized water, and finally add the water-soluble polymer material and functional components; The interactive cavity of the microfluidizer has an aperture of 75-150 μm, is made of diamond, has an emulsification temperature of 10-25° C., and takes 30-60 minutes.

[0036] After the microfluidization emulsification in step (2), cross-linking curing and freeze-drying are further included in sequence. The specific steps are: adding 0.1-0.5% polyethylene glycol diacrylate to the microemulsion suspension, curing for 10-20 minutes under ultraviolet light with a wavelength of 365nm; freeze-drying the tea tree oil microemulsion inclusions after cross-linking and curing, and the specific parameters are: pre-freezing temperature of -40°C to -50°C, pre-freezing time of 4-6 hours, and sublimation drying at a vacuum degree of ≤10Pa for 24-48 hours.

[0037] The invention discloses an application of a tea tree oil microemulsion inclusion body in an anti-acne patch. The tea tree oil microemulsion inclusion body is used to prepare the anti-acne patch, and the anti-acne patch is used for treating acne.

[0038] The technical solution of the present invention is further illustrated below by examples and comparative examples, but the protection scope of the present invention is not limited thereto.

[0039] Example 1 During the supercritical CO2 extraction stage, the CO2 flow rate was set at 40 L / h, the extraction pressure at 30 MPa, and the extraction temperature at 40°C for 4 hours. The volume ratio of the entraining agent, ethanol, to CO2 was adjusted to 1:5. During the microfluidization emulsification stage, polyglycerol-10 laurate and Tween-80 were mixed in a mass ratio of 3:1, and hydroxypropyl methylcellulose and sodium alginate were added in a ratio of 4:1. Sodium hyaluronate was used as the water-soluble polymer, and the functional components included 0.3% dipotassium glycyrrhizate, 0.6% niacinamide, and 0.4% Centella asiatica extract. The microfluidizer pressure was set at 150 MPa, and the process was repeated three times. The pore size of the interaction chamber was 150 μm. The emulsification temperature was maintained at 10°C for 60 minutes. During the cross-linking and curing stage, 0.5% polyethylene glycol diacrylate was added, and UV curing was performed for 20 minutes. Freeze-drying parameters included a pre-freezing temperature of -50°C and vacuum drying for 48 hours.

[0040] Example 2 The extraction process employed a CO2 flow rate of 20 L / h, a pressure of 35 MPa, a temperature of 50°C, an extraction time of 2 hours, and an ethanol to CO2 volume ratio of 1:10. The emulsifier consisted of a 1:1 mass ratio of polyglycerol-10 laurate to Tween-80, and a 2:1 mixture of hydroxypropyl methylcellulose and sodium alginate as the stabilizer. The water-soluble polymer was carboxymethyl chitosan with an 85% deacetylation degree. The functional components included 0.1% dipotassium glycyrrhizate, 0.2% niacinamide, and 0.2% Centella asiatica extract. The microfluidization pressure was increased to 180 MPa, and the process was repeated six times. The cavity aperture was 75 μm, the emulsification temperature was 25°C, and the extraction time was 30 minutes. The crosslinker, polyethylene glycol diacrylate, was used at a concentration of 0.1%, the curing time was 10 minutes, the pre-freezing temperature was -40°C, and the drying time was 24 hours.

[0041] Example 3 The supercritical CO2 flow rate was set at 30 L / h, the pressure at 25 MPa, the temperature at 45°C, the extraction time at 3 hours, and the volume ratio of ethanol to CO2 was 1:7. The emulsifier weight ratio was 2:1, the stabilizer hydroxypropyl methylcellulose to sodium alginate ratio was 3:1, and the water-soluble polymer material was sodium hyaluronate. Functional components included 0.2% dipotassium glycyrrhizate, 0.4% niacinamide, and 0.3% Centella asiatica extract. The microfluidizer pressure was 120 MPa, the treatment cycle was repeated four times, the cavity aperture was 110 μm, the emulsification temperature was 18°C, and the extraction time was 45 minutes. The crosslinker dosage was 0.3%, UV curing was performed for 15 minutes, the pre-freezing temperature was -45°C, and vacuum drying was performed for 36 hours.

[0042] Comparative Example 1

[0043] The similarities between this comparative example and Example 1 are not repeated here, and the differences are as follows: the mass ratio of polyglycerol-10 laurate to Tween-80 is adjusted to 4:1.

[0044] Comparative Example 2

[0045] The similarities between this comparative example and Example 2 are not repeated here, and the differences are as follows: the viscosity of hydroxypropyl methylcellulose is changed to 3000 mPa·s.

[0046] Comparative Example 3

[0047] The same points as in Example 3 are not described in detail here, and the differences are as follows: The microjet pressure was increased to 200 MPa.

[0048] Comparative Example 4

[0049] The same points as in Example 1 are not described in detail here, and the differences are as follows: The water-soluble polymer material only uses sodium hyaluronate, without adding carboxymethyl chitosan.

[0050] Comparative Example 5

[0051] The same points as in Example 2 are not described in detail here, and the differences are as follows: Eliminate the UV cross-linking curing step.

[0052] Performance testing and effect analysis

[0053] The performance of the tea tree oil microemulsion inclusions prepared in the three examples and five comparative examples was tested according to a general method. The test results are shown in Table 1.

[0054] As can be seen in Table 1, comparative testing revealed that Examples 1-3 significantly outperformed the comparative examples in terms of encapsulation efficiency, particle size control, and stability. Example 1 achieved an encapsulation efficiency of 94.2%, a particle size of 58 nm, and a skin irritation index of only 0.12. However, the imbalance in the emulsifier ratio in Comparative Example 1 resulted in a decrease in encapsulation efficiency to 81.4%, and an increase in particle size to 235 nm. The lack of a crosslinking process (Comparative Example 5) increased volatile losses to 27.8%, while Example 1 controlled volatile losses to 8.3% through UV crosslinking and freeze-drying. The synergistic effect of the functional components was particularly evident in the examples: Example 1 achieved a 98.5% inhibition rate against P. acnes, while Comparative Example 4, lacking the cationic antimicrobial activity of carboxymethyl chitosan, saw its inhibition rate drop to 89.3%.

[0055] In Example 1, the 3:1 ratio of polyglycerol-10 laurate to Tween-80 synergistically increased the encapsulation efficiency to 94.2% through hydrophobic interaction and steric hindrance. However, the 4:1 ratio in Comparative Example 1 disrupted this balance, resulting in loose molecular arrangement and a decreased encapsulation efficiency. In Example 3, a pressure of 120 MPa combined with an intermediate number of cycles (4) stabilized the particle size at 123 nm. However, the 200 MPa pressure in Comparative Example 3 exceeded the equipment's tolerance, resulting in uneven oil droplet breakage and an increase in particle size to 521 nm. In Example 2, UV crosslinking formed a dense network structure, anchoring tea tree oil molecules at the crosslinking points. This resulted in a 28-day volatile loss of only 9.8%, while the uncrosslinked microemulsion in Comparative Example 5, lacking a fixed structure, experienced a volatile loss of 27.8%. In Example 1, 0.3% dipotassium glycyrrhizate was used in combination with carboxymethyl chitosan to inhibit the secretion of the inflammatory factor IL-6 by 75%, which was significantly improved compared with the comparative example 4 in which only sodium hyaluronate was used, confirming the synergistic effect of the cationic material and the anti-inflammatory component.

[0056] Table 1 Performance test results

[0057] This application achieves efficient nano-scale encapsulation of tea tree oil through the synergistic effect of composite emulsifiers, supercritical extraction and microfluidic emulsification process, and UV cross-linking curing technology, combining strong transdermal permeability, low irritation, long-lasting sustained release and multiple synergistic effects, breaking through the technical bottleneck of low oil loading and poor stability of traditional anti-acne patches.

[0058] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A tea tree oil microemulsion inclusion body, characterized in that: The tea tree oil microemulsion inclusions include the following components in percentage by mass: 5-15% tea tree oil, 3-8% emulsifier, 1-5% stabilizer, 2-10% water-soluble polymer material, and the balance deionized water; The tea tree oil is extracted by a supercritical CO2 extraction method, and the average particle size of the tea tree oil microemulsion inclusions is 50-200nm.

2. The tea tree oil microemulsion inclusion according to claim 1, wherein: The emulsifier is a mixture of polyglycerol-10 laurate and Tween-80, and the mass ratio of the emulsifier to polyglycerol-10 laurate and Tween-80 is 1-3:

1.

3. The tea tree oil microemulsion inclusion according to claim 1, characterized in that: The stabilizer is a mixture of hydroxypropyl methylcellulose and sodium alginate, the mass ratio of the hydroxypropyl methylcellulose to the sodium alginate is 2:1-4:1, and the viscosity of the hydroxypropyl methylcellulose is 4000-6000 mPa·s.

4. The tea tree oil microemulsion inclusion according to claim 1, characterized in that: The water-soluble polymer material is sodium hyaluronate or carboxymethyl chitosan, and the deacetylation degree of the carboxymethyl chitosan is ≥85%.

5. The tea tree oil microemulsion inclusion according to claim 1, characterized in that: The tea tree oil microemulsion inclusion body further comprises functional components, which include: 0.1-0.3% dipotassium glycyrrhizate, 0.2-0.6% niacinamide, and 0.2-0.4% Centella asiatica extract.

6. A method for preparing the tea tree oil microemulsion inclusions according to any one of claims 1 to 5, characterized in that: The method comprises the following steps: (1) Supercritical CO2 extraction of tea tree oil: crush the branches and leaves of Melaleuca alternifolia and put them into the extraction kettle, control the CO2 flow rate to 20-40L / h, extraction pressure to 25-35MPa, temperature to 40-50℃, extraction time to 2-4h, and obtain tea tree oil; (2) Microfluidization: The tea tree oil obtained in step (1) is mixed with an emulsifier, a stabilizer, and a water-soluble polymer material in the mass percentages according to claim 1, deionized water is added, and the mixture is circulated in a microfluidizer at a pressure of 120-180 MPa for 3-6 times to obtain tea tree oil microemulsion inclusions with an average particle size of 50-200 nm.

7. The method for preparing a tea tree oil microemulsion inclusion according to claim 6, wherein: In step (1), the supercritical CO2 extraction process further adds entrainer ethanol, the volume ratio of the ethanol to CO2 is 1:10-1:5, and the purity of the ethanol is ≥99.5%.

8. The method for preparing a tea tree oil microemulsion inclusion body according to claim 6, wherein: The mixing process of step (2) also includes adding functional components: dipotassium glycyrrhizate 0.1-0.3%, niacinamide 0.2-0.6%, and Centella asiatica extract 0.2-0.4%; The order of addition during the mixing process is: first add the emulsifier and premix with tea tree oil, then add the stabilizer and deionized water, and finally add the water-soluble polymer material and functional components; The interactive cavity of the microfluidizer has an aperture of 75-150 μm, is made of diamond, has an emulsification temperature of 10-25° C., and takes 30-60 minutes.

9. The method for preparing a tea tree oil microemulsion inclusion according to claim 6, wherein: After the microfluidization emulsification in step (2), cross-linking curing and freeze-drying are further included in sequence. The specific steps are: adding 0.1-0.5% polyethylene glycol diacrylate to the microemulsion suspension, curing for 10-20 minutes under ultraviolet light with a wavelength of 365nm; freeze-drying the tea tree oil microemulsion inclusions after cross-linking and curing, and the specific parameters are: pre-freezing temperature of -40°C to -50°C, pre-freezing time of 4-6 hours, and sublimation drying at a vacuum degree of ≤10Pa for 24-48 hours.

10. Use of the tea tree oil microemulsion inclusions according to any one of claims 1 to 5 in an anti-acne patch, characterized in that: The tea tree oil microemulsion inclusion body is used to prepare an anti-acne patch, and the anti-acne patch is used for treating acne.

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