White tomato extract and mannitol eutectic crystal as well as preparation method and application thereof

Abstract

The invention relates to the technical field of eutectic crystals, in particular to a white tomato extract and mannitol eutectic crystal and a preparation method and application thereof.The XRD spectrum of the eutectic crystal at least has characteristic diffraction peaks at the positions where the 2 theta angle is 13.76 degrees, 17.32 degrees, 17.44 degrees, 18.80 degrees, 19.92 degrees, 20.48 degrees, 21.43 degrees, 25.19 degrees, 27.05 degrees, 27.59 degrees, 28.21 degrees, 33.21 degrees, 34.02 degrees, 35.61 degrees, 36.25 degrees and 43.97 degrees, and the error tolerance is + / -0.2 degrees; characteristic endothermic peaks exist at 164.3 DEG C and 332.7 DEG C in a DSC spectrum, and error tolerance of + / -0.2 DEG C exists; the preparation method comprises the following steps: dissolving mannitol and the white tomato extract in water, and carrying out ultrasonic treatment, standing, crystallization, filtration, vacuum drying and grinding to obtain the eutectic which can be applied to cosmetics, health care products, food and medicines. The eutectic crystal disclosed by the invention has the advantages of no irritation, high safety, good transdermal absorption and good stability.

Description

Technical Field

[0001] The present invention relates to the technical field of co-crystals, and in particular to a co-crystal of white tomato extract and mannitol, and a preparation method and application thereof. Background Art

[0002] White tomato extract is an active ingredient extracted from white tomato fruits. It is rich in colorless carotenoids (such as phytoene, phytoene, and ζ-carotenoids), which have powerful antioxidant, UV protection, and whitening properties. Compared to regular tomatoes, the carotenoids in white tomato extract are colorless and do not deposit pigments in the skin. They are also more photostable and can effectively reduce UV-induced melanin production. Therefore, it is widely used in skin care, health care, and nutritional supplements. However, the carotenoids in tomato extract are sensitive to light and heat, which results in poor stability. The large molecular structure of tomato extract may also result in low transdermal absorption.

[0003] Mannitol is a six-carbon sugar alcohol found widely in plants such as kelp and mushrooms. It can also be produced through chemical synthesis. Mannitol has moisturizing, antioxidant, soothing, high permeability, low calorie content, and no toxic side effects. It is widely used in medicine, food, cosmetics, and health supplements. Summary of the Invention

[0004] In response to the problems in the prior art that white tomato extracts have certain irritation and poor transdermal absorption and stability, the present invention proposes a white tomato extract and mannitol co-crystal and its preparation method and application. The obtained co-crystal has the advantages of being non-irritating, highly safe, having good transdermal absorption and good stability.

[0005] Specifically, the present invention is achieved through the following technical solution: a co-crystal of a white tomato extract and mannitol, wherein the XRD pattern of the co-crystal of the white tomato extract and mannitol has characteristic diffraction peaks at least at 2θ angles of 13.76°, 17.32°, 17.44°, 18.80°, 19.92°, 20.48°, 21.43°, 25.19°, 27.05°, 27.59°, 28.21°, 33.21°, 34.02°, 35.61°, 36.25°, and 43.97°, with an error tolerance of ±0.2°; and the DSC spectrum has characteristic endothermic peaks at 164.3°C and 332.7°C, with an error tolerance of ±0.2°.

[0006] As a preferred embodiment, the XRD pattern of the cocrystal of the white tomato extract and mannitol also has characteristic diffraction peaks at 2θ angles of 9.51°, 11.06°, 25.78°, 28.65°, 30.47°, 31.12°, 31.57°, 36.70°, 38.26°, 39.64°, 40.85°, 44.73°, 48.38°, and 56.64°, with an error tolerance of ±0.2°; the grain sizes corresponding to the characteristic diffraction peaks are

[0007] As a preferred embodiment, the grain sizes corresponding to the characteristic diffraction peaks of 13.76°, 17.32°, 17.44°, 18.80°, 19.92°, 20.48°, 21.43°, 25.19°, 27.05°, 27.59°, 28.21°, 33.21°, 34.02°, 35.61°, 36.25°, and 43.97° are respectively

[0008] As a preferred embodiment, the infrared spectrum of the white tomato extract and mannitol cocrystal is at least 3374.2 cm -1 、3282.92cm -1 、2934.47cm -1 、1458.62cm -1 、1430.66cm -1 、1376.13cm -1 、1318.07cm -1 、1259.22cm -1 、1195.09cm -1 、1078.39cm -1 、1016.9cm -1 、949.13cm -1 、928.97cm -1 、878.16cm -1 、716.21cm -1 、625.71cm -1 There is a characteristic peak at ±0.2cm -1 error tolerance.

[0009] The present invention also provides a method for preparing a co-crystal of white tomato extract and mannitol, comprising the following steps:

[0010] S1. Weigh mannitol and pure water according to the ratio, and stir to dissolve mannitol in the pure water to form a mannitol solution;

[0011] S2. Weigh the white tomato extract according to the ratio and add it to the mannitol solution in S1. Heat and stir until the white tomato extract is completely dissolved.

[0012] S3, sonicating the solution in step S2 for 2-4 hours;

[0013] S4, taking out the reaction solution in S3 and letting it stand to wait for crystallization;

[0014] S5. Filter the crystals in S4 and vacuum dry them to obtain a crystalline powder of white tomato extract and mannitol.

[0015] Furthermore, the mass ratio of mannitol, white tomato extract and pure water is 2:1:7.

[0016] Furthermore, in step S2, stirring is performed in a heat-collecting constant temperature heating magnetic stirrer at a temperature of 30 to 50° C. and a rotation speed of 200 r / min, and stirring is continued for 1 to 2 hours until the white tomato extract is completely dissolved.

[0017] Furthermore, in step S3, the ultrasonic power is 300w-600w, and the ultrasonic frequency is 20-50khz.

[0018] Furthermore, in step S5, the vacuum degree is 50Pa-70Pa, and the temperature is 30-40°C.

[0019] The last aspect of the present invention also provides the use of the white tomato extract and mannitol co-crystal in cosmetics, food, health products and medicines.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] (1) Compared with the raw material white tomato extract, the white tomato extract and mannitol cocrystal prepared by the present invention has no skin irritation according to the chicken embryo test results, and the zebrafish test results show that its safety is better than that of white tomato extract, thereby effectively reducing the skin redness, itching and other allergic discomfort symptoms that may occur in cosmetics and applications, greatly improving the product safety in health products, food, and pharmaceutical applications;

[0022] (2) The surface release test on miniature pig skin showed that the transdermal absorption effect of the white tomato extract and mannitol co-crystal prepared by the present invention was better than that of the white tomato extract. This may be because the co-crystal optimized the intermolecular interactions and arrangement, thereby improving its ability to penetrate the skin barrier, thereby promoting the full play of the multiple effects of the white tomato extract and mannitol, such as whitening, antioxidant, moisturizing, antibacterial, soothing and calming.

[0023] (3) Compared with the raw material white tomato extract, the stability of the white tomato extract and mannitol co-crystal prepared by the present invention is significantly improved, so that the active ingredients can be kept stable and not easily deteriorated in the application of cosmetics, food, health products, and medicines, which also provides the possibility of extending the shelf life of the product. The highly stable co-crystal can ensure that the white tomato extract and mannitol can continue to exert their multiple effects such as whitening, anti-oxidation, moisturizing, antibacterial, soothing and calming in skin care products. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is the XRD pattern of the white tomato extract, mannitol, and the co-crystal of the white tomato extract and mannitol of the present invention;

[0025] Figure 2 is a DSC graph of the co-crystal of the white tomato extract and mannitol of the present invention;

[0026] Figure 3 It is the DSC diagram of mannitol of the present invention;

[0027] Figure 4 is a DSC graph of the white tomato extract of the present invention;

[0028] Figure 5 This is the infrared spectrum of the co-crystal of the white tomato extract and mannitol of the present invention;

[0029] Figure 6 This is a graph showing the irritation test of the white tomato extract and mannitol co-crystal of the present invention (chicken embryo test results);

[0030] Figure 7 This is a graph showing the irritation test of the white tomato extract of the present invention (chicken embryo test results);

[0031] Figure 8 This is a comparative data chart of transdermal absorption of mannitol white tomato extract co-crystal and white tomato extract of the present invention;

[0032] Figure 9 This is a stability test chart of the mannitol white tomato extract co-crystal and the white tomato extract solution of the present invention. DETAILED DESCRIPTION

[0033] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, rather than all the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0034] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0035] Unless otherwise specifically stated, the relative arrangement of the parts and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention. The technology, methods and equipment known to those of ordinary skill in the relevant art may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be considered as part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that similar numbers and letters represent similar items in the following figures, and therefore, once an item is defined in one figure, it does not need to be further discussed in subsequent figures.

[0036] Example 1

[0037] A method for preparing a co-crystal of white tomato extract and mannitol comprises the following steps:

[0038] S1. Weigh mannitol and pure water according to the ratio, and stir to dissolve mannitol in the pure water to form a mannitol solution;

[0039] S2. Weigh the white tomato extract and add it to the mannitol solution in S1. Stir the mixed solution in a heat-collecting constant temperature heating magnetic stirrer at 40°C and 200 rpm for 1.5 h until it is completely dissolved.

[0040] S3, placing the solution in step S2 in an ultrasonic instrument for 3 hours, with an ultrasonic power of 450W and an ultrasonic frequency of 35kHz;

[0041] S4. Take out the reaction solution in S3 and let it stand for 18 hours to wait for crystallization;

[0042] S5. Filter the crystals in S4, and then vacuum dry them at 35° C. and a vacuum degree of 60 Pa to obtain a co-crystal powder of white tomato extract and mannitol.

[0043] The mass ratio is shown in the following table:

[0044] Material Name Ratio White Tomato Extract 10％ Mannitol 20％ pure water 70％

[0045] Example 2

[0046] A method for preparing a co-crystal of white tomato extract and mannitol comprises the following steps:

[0047] S1. Weigh mannitol and pure water according to the ratio, and stir to dissolve mannitol in the pure water to form a mannitol solution;

[0048] S2. Weigh the white tomato extract and add it to the mannitol solution in S1. Stir the mixed solution in a heat-collecting constant temperature heating magnetic stirrer at 50°C and 200 rpm. Stir continuously for 1 hour until the mixture is completely dissolved.

[0049] S3, placing the solution in step S2 in an ultrasonic instrument for 2 hours, with an ultrasonic power of 600w and an ultrasonic frequency of 50kHz;

[0050] S4, take out the reaction solution in S3 and let it stand for 24 hours to wait for crystallization;

[0051] S5. Filter the crystals in S4, and then dry them in vacuo at 30° C. and a vacuum degree of 70 Pa to obtain a powder of cocrystal of white tomato extract and mannitol.

[0052] The mass ratio is shown in the following table:

[0053] Material Name Ratio White Tomato Extract 10％ Mannitol 20％ pure water 70％

[0054] Example 3

[0055] A method for preparing a co-crystal of white tomato extract and mannitol comprises the following steps:

[0056] S1. Weigh mannitol and pure water according to the ratio, and stir to dissolve mannitol in the pure water to form a mannitol solution;

[0057] S2. Weigh the white tomato extract and add it to the mannitol solution in S1. Stir the mixed solution in a heat-collecting constant temperature heating magnetic stirrer at 30°C and 200 rpm for 2 h until the mixture is completely dissolved.

[0058] S3, placing the solution in step S2 in an ultrasonic instrument for 4 hours, ultrasonic power 300w, ultrasonic frequency 20khz;

[0059] S4, take out the reaction solution in S3 and let it stand for 24 hours to wait for crystallization;

[0060] S5. Filter the crystals in S4, and then dry them in vacuo at 40° C. and a vacuum degree of 50 Pa to obtain a powder of the cocrystal of white tomato extract and mannitol.

[0061] The mass ratio is shown in the following table:

[0062] Material Name Ratio White Tomato Extract 10％ Mannitol 20％ pure water 70％

[0063] X-ray diffraction analysis was performed on the co-crystal of white tomato extract and mannitol obtained in Example 1 and the raw materials. The specific test parameters were:

[0064] Voltage, current: 40kV, 40mA

[0065] Detector: DteX250(H)

[0066] Test range: 5-80°

[0067] Step size: 0.01°

[0068] Scanning speed: 10.00deg / min

[0069] Divergence slit: 10mm;

[0070] Get as Figure 1 The XRD pattern shown in the figure shows that the XRD pattern of the cocrystal of white tomato extract and mannitol has characteristic diffraction peaks at least at 2θ angles of 13.76°, 17.32°, 17.44°, 18.80°, 19.92°, 20.48°, 21.43°, 25.19°, 27.05°, 27.59°, 28.21°, 33.21°, 34.02°, 35.61°, 36.25°, and 43.97°, with an error tolerance of ±0.2°. The grain sizes corresponding to the characteristic diffraction peaks are

[0071] The DSC spectrum has a characteristic endothermic peak at ℃ with an error tolerance of ±0.2℃.

[0072] The XRD pattern of the cocrystal of white tomato extract and mannitol also has characteristic diffraction peaks at 2θ angles of 9.51°, 11.06°, 25.78°, 28.65°, 30.47°, 31.12°, 31.57°, 36.70°, 38.26°, 39.64°, 40.85°, 44.73°, 48.38°, and 56.64°, with an error tolerance of ±0.2°; the grain sizes corresponding to each characteristic diffraction peak are

[0073] White tomato extract has a characteristic diffraction peak at a 2θ angle of 40.5° with an error tolerance of ±0.2°; mannitol mainly has characteristic diffraction peaks at 2θ angles of 14.80°, 18.98°, 21.31°, 23.55°, 23.69°, 28.58°, 33.77°, and 38.92°.

[0074] from Figure 1It can be seen from the figure that the characteristic diffraction peaks of the eutectic are different from those of the raw materials, indicating that the eutectic is obtained.

[0075] The specific XRD data of the obtained white tomato extract and mannitol co-crystal are shown in the following table:

[0076] Table 1 XRD data of white tomato extract and mannitol cocrystal

[0077]

[0078]

[0079] The white tomato extract and mannitol cocrystal obtained in Example 1 and the raw materials were subjected to DSC test. The test parameters were as follows: temperature 50-400°C, heating rate 10.0°C / min, Ar 20.0mL / min. The results are as follows Figure 2-4 The temperature corresponding to the peak in the figure is the temperature inside the high-temperature furnace of the instrument. Since the sample temperature is lower than the furnace temperature at the same time, the melting temperature of the sample is analyzed by software as follows:

[0080] 1) White tomato extract: White tomato extract has a melting peak at 242.76°C;

[0081] 2) Mannitol: melting peak at 169.2°C;

[0082] 3) Co-crystal of white tomato extract and mannitol: There are two melting peaks, 164.35℃ and 332.77℃, which are different from the peak positions of the raw material, indicating that a co-crystal was obtained.

[0083] The white tomato extract obtained in Example 1 and the mannitol cocrystal were subjected to infrared testing to obtain the following Figure 5 The infrared spectrum shown, Figure 5 The eutectic is shown at 3374.2 cm -1 、3282.92cm -1 、2934.47cm -1 、1458.62cm -1 、1430.66cm -1 、1376.13cm -1 、1318.07cm -1 、1259.22cm -1 、1195.09cm -1 、1078.39cm -1 、1016.9cm -1 、949.13cm -1 、928.97cm -1 、878.16cm -1 、716.21cm-1 、625.71cm -1 There is a characteristic peak at ±0.2cm -1 error tolerance.

[0084] Example 4 Efficacy Test (Sample 1# is the eutectic obtained in Example 1):

[0085] 1. Irritation test:

[0086] Test method: Chicken chorioallantoic membrane test for eye irritation / corrosion of cosmetics (SN / T 2329)

[0087] Determination of acute toxicity of water substances to freshwater fish (zebrafish) (GB / T13267-91)

[0088] Testing instruments: stereo microscope, fully automatic incubator

[0089] Sample Information:

[0090]

[0091] The results of the chicken embryo test are as follows:

[0092]

[0093] The zebrafish test results are as follows:

[0094]

[0095] According to the above chicken embryo test results tabular data and Figure 6 and Figure 7 It can be seen that 1.0% white tomato extract and mannitol cocrystal is non-irritating, and white tomato extract is slightly irritating; the zebrafish test results show that the safety of white tomato extract and mannitol cocrystal is better than that of raw white tomato extract.

[0096] 2. Transdermal absorption test

[0097] Sample Information:

[0098]

[0099]

[0100] (1) Test method for release from the surface of miniature pig skin:

[0101] Pig skin model: Xiaoba fragrant pig skin

[0102] Supply liquid: 1# white tomato extract and mannitol eutectic solution, 2# white tomato extract solution

[0103] Experimental methods:

[0104] Diffusion cell installation and skin condition testing:

[0105] Without adding receiving solution, use filter paper to absorb the moisture of the skin (weigh it) and place it on the top surface of the receiving pool with the skin stratum corneum facing up. Add water to the supply pool to test whether there is liquid infiltration in the receiving pool. If there is no infiltration, discard the saline in the supply pool and wipe the water on the supply pool and skin surface;

[0106] The sample receiving solution was balanced by adding 5 mL of physiological saline solution (weighed) until it was in complete contact with the skin and no bubbles were expelled. The sample was then placed in a water bath for 30 minutes to balance.

[0107] Add sample supply solution: Take 400 μL of 1# and 2# supply solution respectively and add them to the supply pool;

[0108] The diffusion cell temperature was 34°C and the stirring speed was 450 rpm.

[0109] Sampling test after 4 hours:

[0110] ① Supply liquid: weigh the total weight of the supply pool (place it in a 25mL beaker or centrifuge tube, the gross weight of the supply pool needs to be weighed in advance), rinse with 50% methanol and dilute to 10mL, shake it by ultrasonic for 20min, filter and ultrasonically degas;

[0111] ② Skin surface: Weigh the total weight (the skin weight needs to be weighed in advance), rinse with 50% methanol or wipe the surface with a cotton swab and dilute to 2 mL, shake well, filter and ultrasonically degas;

[0112] ③Inside the skin: mince the skin, add 2 mL of 50% methanol and sonicate for 20 minutes;

[0113] ④ Receiving solution: Take 1 mL of receiving solution, filter it, and then ultrasonically degas it for 1 minute before injection.

[0114] (2) White tomato extract transdermal absorption test method:

[0115] Instrument: Xevo G3-Q TOF liquid chromatography-mass spectrometry;

[0116] Chromatographic column: ACQUITY UPLC HSS BEH C18 (2.1*50 mm, 1.7 μm);

[0117] Mobile phase: Phase A 0.1% formic acid aqueous solution Phase B methanol: dichloromethane = 97:3;

[0118] Elution method: gradient method; B phase ratio: 0-3min, 92%; 4.5-15min, 100%;

[0119] 15.1-20min, 92%;

[0120] Flow rate: 0.4 mL / min;

[0121] Mass spectrometry conditions:

[0122] Negative ion mode, capillary voltage 2.5 kV, cone voltage 40 V, source default voltage 80 V, source temperature 120 °C, desolvation temperature 450 °C, carrier gas flow rate 50 L / H, desolvation gas flow rate 900 L / H;

[0123] Positive ion mode, capillary voltage 2.5 kV, cone voltage 40 V, source default voltage 80 V, source temperature 120 °C, desolvation temperature 450 °C, carrier gas flow rate 50 L / H, desolvation gas flow rate 800 L / H.

[0124] Test results:

[0125]

[0126]

[0127] From the above test results and Figure 8 It can be seen that with phytoene as the target and miniature pig skin as the model, it was found that the transdermal absorption of mannitol and white tomato extract cocrystal was better than that of white tomato extract.

[0128] 3. Stability test

[0129] White tomato extract content test method:

[0130] High performance liquid chromatograph: Waters E2695 high performance liquid chromatograph;

[0131] Chromatographic column: Wondasil C18-WR (150*4.6mm, 5μm);

[0132] Mobile phase: methanol-tetrahydrofuran (volume ratio 75:25);

[0133] Elution method: isocratic elution;

[0134] Flow rate: 1 mL / min;

[0135] Temperature: 30℃;

[0136] Detection wavelength: 287nm.

[0137] Test results:

[0138]

[0139] from Figure 9As can be seen from the data in the above table, taking the content of white tomato extract as an indicator, the 48-hour high-temperature stability test found that the stability of the mannitol and white tomato extract co-crystal was better than that of the white tomato extract.

[0140] The above are preferred embodiments of the present invention. Those skilled in the art to which the present invention belongs can also change and modify the above embodiments. Therefore, the present invention is not limited to the above specific embodiments. Any obvious improvements, replacements or modifications made by those skilled in the art on the basis of the present invention fall within the scope of protection of the present invention.

Claims

1. A co-crystal of white tomato extract and mannitol, characterized by: The XRD spectrum of the white tomato extract and mannitol cocrystal has characteristic diffraction peaks at least at 2θ angles of 13.76°, 17.32°, 17.44°, 18.80°, 19.92°, 20.48°, 21.43°, 25.19°, 27.05°, 27.59°, 28.21°, 33.21°, 34.02°, 35.61°, 36.25°, and 43.97°, with an error tolerance of ±0.2°; the DSC spectrum has characteristic endothermic peaks at 164.3°C and 332.7°C, with an error tolerance of ±0.2°.

2. The co-crystal of white tomato extract and mannitol according to claim 1, characterized in that: The XRD pattern of the white tomato extract and mannitol cocrystal also has characteristic diffraction peaks at 2θ angles of 9.51°, 11.06°, 25.78°, 28.65°, 30.47°, 31.12°, 31.57°, 36.70°, 38.26°, 39.64°, 40.85°, 44.73°, 48.38°, and 56.64°, with an error tolerance of ±0.2°; The grain sizes corresponding to the characteristic diffraction peaks are 3. The co-crystal of white tomato extract and mannitol according to claim 1, characterized in that: The grain sizes corresponding to the characteristic diffraction peaks of 13.76°, 17.32°, 17.44°, 18.80°, 19.92°, 20.48°, 21.43°, 25.19°, 27.05°, 27.59°, 28.21°, 33.21°, 34.02°, 35.61°, 36.25°, and 43.97° are 4. The co-crystal of white tomato extract and mannitol according to claim 1, characterized in that: The infrared spectrum of the cocrystal of white tomato extract and mannitol is at least 3374.2 cm -1 、3282.92cm -1 、2934.47cm -1 、1458.62cm -1 、1430.66cm -1 、1376.13cm -1 、1318.07cm -1 、1259.22cm -1 、1195.09cm -1 、1078.39cm -1 、1016.9cm -1 、 949.13cm -1 、928.97cm -1 、878.16cm -1 、716.21cm -1 、625.71cm -1 There is a characteristic peak at ±0.2cm -1 error tolerance.

5. The method for preparing the co-crystal of white tomato extract and mannitol according to any one of claims 1 to 4, characterized in that: Here are the steps: S1. Weigh mannitol and pure water according to the ratio, and stir to dissolve mannitol in the pure water to form a mannitol solution; S2. Weigh the white tomato extract according to the ratio and add it to the mannitol solution in S1. Heat and stir until the white tomato extract is completely dissolved. S3, sonicating the solution in step S2 for 2-4 hours; S4, taking out the reaction solution in S3 and letting it stand to wait for crystallization; S5. Filter the crystals in S4, vacuum-dry, and grind to obtain a crystalline powder of white tomato extract and mannitol.

6. The white tomato extract and mannitol co-crystal and the preparation method thereof according to claim 5, characterized in that: The mass ratio of mannitol, white tomato extract and pure water is 2:1:

7.

7. The method for preparing the co-crystal of white tomato extract and mannitol according to claim 5, characterized in that: In step S2, stirring is performed in a heat-collecting constant temperature heating magnetic stirrer at a temperature of 30 to 50° C. and a rotation speed of 200 r / min, and stirring is continued for 1 to 2 hours until the white tomato extract is completely dissolved.

8. The method for preparing the co-crystal of white tomato extract and mannitol according to claim 5, characterized in that: In step S3, the ultrasonic power is 300w-600w, and the ultrasonic frequency is 20-50khz.

9. The method for preparing the co-crystal of white tomato extract and mannitol according to claim 5, characterized in that: In step S5, the vacuum degree is 50Pa-70Pa and the temperature is 30-40°C.

10. Use of the white tomato extract and mannitol co-crystal according to any one of claims 1 to 4 in cosmetics, foods, health products and medicines.

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