Nucleic acid amplification reaction card box

Abstract

The invention discloses a nucleic acid amplification reaction card box which is used for being inserted into a reaction instrument to carry out nucleic acid amplification reaction. The nucleic acid amplification reaction card box comprises a card box body and a sealing cover, the sealing cover is used for sealing the card box body to guarantee the sealing reaction of the card box body, a sample adding pool used for adding reaction liquid is arranged at the top of the card box body, the card box body is communicated with the first sample feeding channel through the sample adding pool, and in the process that the sealing cover seals the sample adding pool, the first sample feeding channel is communicated with the second sample feeding channel. By extruding air at the top of the sample adding pool, the reaction liquid in the sample adding pool can be driven to flow along the first sample introduction channel, so that the dependence of an external device on the reaction liquid driving process is reduced, the convenience of nucleic acid detection is improved, and the nucleic acid detection efficiency is improved.

Description

Technical Field

[0001] The present application relates to the field of nucleic acid detection technology, and more specifically, to a nucleic acid amplification reaction cartridge. Background Art

[0002] Currently, the nucleic acid amplification process is usually implemented using microfluidic technology. However, existing microfluidic nucleic acid amplification equipment usually relies on external devices to drive the reaction liquid, making the nucleic acid detection process more cumbersome and resulting in low efficiency of nucleic acid detection.

[0003] In summary, how to improve the convenience of nucleic acid detection to improve the efficiency of nucleic acid detection is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0004] In view of this, the purpose of this application is to provide.

[0005] In order to achieve the above objectives, this application provides the following technical solutions:

[0006] A nucleic acid amplification reaction cartridge, for insertion into a reactor, comprises: a cartridge body and a sealing cover; wherein the sealing cover is used to seal the cartridge body; a sample loading reservoir is provided on the top of the cartridge body, the sample loading reservoir being connected to a first sample injection channel; the sealing cover is used to seal the sample loading reservoir; and the sealing cover can drive the reaction liquid in the sample loading reservoir to flow along the first sample injection channel during the process of sealing the sample loading reservoir.

[0007] In some embodiments, the sample addition pool is connected to the first sample introduction channel through a sample injection hole, and a flow cross section of the first sample introduction channel is much smaller than a cross section of the sample addition pool; and scale lines are provided on an outer surface of the sample addition pool.

[0008] In some embodiments, a first threaded structure is provided on the outside of the sample loading pool; the sealing cover includes: a cap, a second threaded structure is provided on the inner side of the cap, and the second threaded structure is threadedly engaged with the first threaded structure; a plunger, the plunger is fixedly connected to the inside of the cap and protrudes from the cap, and the plunger is sealed and engaged with the sample loading pool.

[0009] In some embodiments, the plunger is made of rubber; or, the plunger is made of silicone.

[0010] In some embodiments, the sample loading pool includes a connected equal diameter section and a tapered section, and the tapered section gradually tapers from the equal diameter section toward the injection hole; the equal diameter section and the tapered section are distributed in sequence along the first direction.

[0011] In some embodiments, the first injection channel is connected to at least two reaction units; the at least two reaction units are distributed sequentially along the flow direction of the reaction liquid in the second direction; and there is an angle between the first direction and the second direction.

[0012] In some embodiments, the reaction unit includes: a second injection channel, the second injection channel is connected to the first injection channel, the second injection channel extends along a first direction, and the flow cross-section of the second injection channel is larger than the flow cross-section of the first injection channel; a reaction chamber, the reaction chamber is connected to the second injection channel; a first exhaust hole, the first exhaust hole is connected to the reaction chamber through the first exhaust channel; the first exhaust hole, the first exhaust channel, the reaction chamber and the second injection channel are distributed in sequence along the first direction.

[0013] In some embodiments, in at least two of the reaction units, the apertures of the first exhaust holes decrease successively along the direction of flow of the reaction liquid.

[0014] In some embodiments, the end of the first injection channel is connected to a waste liquid pool; the waste liquid pool is connected to a second exhaust hole through a second exhaust channel, and the aperture of the second exhaust hole is smaller than the aperture of the first exhaust hole.

[0015] In some embodiments, the cartridge body includes a first surface and a second surface that are relatively distributed; on the first surface, the injection hole, the first injection channel, the second injection channel, the reaction chamber, the first exhaust channel, the waste liquid pool, and the second exhaust channel are all open structures and are all open facing the first surface and form a reaction area; the first exhaust hole and the second exhaust hole penetrate and connect the first surface and the second surface, and the second surface forms a film-sticking area, and the film-sticking area covers the first exhaust hole and the second exhaust hole.

[0016] In some embodiments, the reaction area is sealed by a first sealing member; and the film-attaching area is sealed by a second sealing member.

[0017] In some embodiments, the second sealing member is a hydrophobic breathable membrane.

[0018] In some embodiments, the reaction instrument is provided with a card slot, and the card box body is used to be plugged into and matched with the card slot; both ends of the card box body in the second direction are provided with a first positioning portion, and the first positioning portion is used to locate the insertion depth of the card box body in the reaction instrument; at least one end of the card box body in the second direction is provided with a second positioning portion, and the second positioning portion extends along the first direction, and the reaction instrument is provided with at least one positioning slot, and the second positioning portion is used to be positioned and matched with the positioning slot.

[0019] The nucleic acid amplification reaction cartridge provided in the present application is used to be inserted into a reactor for performing a nucleic acid amplification reaction; the nucleic acid amplification reaction cartridge includes a cartridge body and a sealing cover, the sealing cover is used to seal the cartridge body to ensure a sealed reaction of the cartridge body, a sample loading reservoir for adding a reaction liquid is provided on the top of the cartridge body, and the sample loading reservoir is connected to a first sample injection channel, and in the process of sealing the sample loading reservoir with the sealing cover, the reaction liquid in the sample loading reservoir can be driven to flow along the first sample injection channel by squeezing the air on the top of the sample loading reservoir, thereby reducing dependence on an external device for driving the reaction liquid, improving the convenience of nucleic acid detection, and improving the efficiency of nucleic acid detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are merely embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without any creative work.

[0021] Figure 1 A schematic diagram of the structure of the nucleic acid amplification reaction cartridge provided in an embodiment of the present application;

[0022] Figure 2 A front view of the nucleic acid amplification reaction cartridge provided in an embodiment of the present application;

[0023] Figure 3 A side cross-sectional view of a nucleic acid amplification reaction cartridge provided in an embodiment of the present application;

[0024] Figure 4 This is a rear view of the nucleic acid amplification reaction cartridge provided in an embodiment of the present application;

[0025] Figure 5 A schematic diagram of the sealing cover structure of the nucleic acid amplification reaction cartridge provided in an embodiment of the present application;

[0026] Figure 6 The detection instrument provided in the embodiment of the present application;

[0027] Figure 7 Schematic diagram of the cooperation between the nucleic acid amplification reaction cartridge and the detection instrument provided in the embodiment of the present application.

[0028] Description of reference numerals:

[0029] 100 - cartridge body, 101 - sample loading reservoir, 1011 - scale mark, 1012 - first thread structure, 102 - injection port, 103 - first injection channel, 104 - second injection channel, 105 - reaction chamber, 106 - first exhaust channel, 107 - first exhaust hole, 108 - waste liquid reservoir, 109 - second exhaust channel, 110 - second exhaust hole, 111 - film application area, 120 - first positioning portion, 130 - second positioning portion;

[0030] 200-sealing cover, 201-cap, 202-plunger, 203-second thread structure;

[0031] 300-first sealing member;

[0032] 400-second sealing member;

[0033] 500-reactor, 501-card slot, 502-positioning slot. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0035] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. The terms used in the following embodiments are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in the specification and claims of this application, the singular expressions "one", "a kind of", "said", "above", "the" and "this" are intended to also include expressions such as "one or more", unless the context clearly indicates otherwise.

[0036] References to "one embodiment" or "some embodiments" in this specification mean that a particular feature, structure, or characteristic described in conjunction with that embodiment is included in one or more embodiments of the present application. Thus, phrases such as "in one embodiment," "in some embodiments," "in other embodiments," and "in yet other embodiments" appearing in various places in this specification do not necessarily refer to the same embodiment, but rather mean "one or more but not all embodiments," unless otherwise specifically emphasized. The terms "including," "comprising," "having," and variations thereof mean "including but not limited to," unless otherwise specifically emphasized.

[0037] The "multiple" involved in the embodiments of the present application means greater than or equal to two. It should be noted that in the description of the embodiments of the present application, the words "first" and "second" are only used for the purpose of distinguishing the description and cannot be understood as indicating or implying relative importance or order.

[0038] The terms "parallel" and "perpendicular" in this application refer to "substantially parallel" and "substantially perpendicular" in actual operation. "Substantially parallel" can be understood as parallel with a certain error, and similarly, "substantially perpendicular" can be understood as perpendicular with a certain error.

[0039] like Figure 1 As shown, the nucleic acid amplification reaction cartridge provided in the embodiment of the present application is used to be inserted into the interior of a reactor 500 to perform a nucleic acid amplification reaction. The nucleic acid amplification reaction cartridge includes a cartridge body 100 and a sealing cover 200. The sealing cover 200 is used to seal the cartridge body 100 to ensure that the cartridge body 100 can perform a sealed reaction in the reactor 500. A sample loading reservoir 101 for adding a reaction liquid is provided on the top of the cartridge body 100. The sample loading reservoir 101 is connected to a first sample injection channel 103, and the sealing cover 200 can seal the sample loading reservoir 101. In the process of the sealing cover 200 sealing the sample loading reservoir 101, the sealing cover 200 squeezes the air on the top of the sample loading reservoir 101, which can drive the reaction liquid in the sample loading reservoir 101 to flow along the first sample injection channel 103, reducing the dependence on external devices for the reaction liquid driving process, improving the convenience of nucleic acid detection, and improving the efficiency of nucleic acid detection.

[0040] It should be noted that the reactor 500 may be an automatic nucleic acid amplification detector, an integrated molecular diagnostic system, or other instrument capable of performing nucleic acid amplification reaction and detection, and this embodiment of the present application does not limit this.

[0041] like Figure 1-Figure 2 As shown, the sample loading pool 101 is connected to the first sample loading channel 103 through the sample loading hole 102, and the flow cross-section of the first sample loading channel 103 is much smaller than the cross-section of the sample loading pool 101. The flow cross-section of the first sample loading channel 103 is extremely thin, so that in the absence of external force, the reaction liquid in the sample loading pool 101 will not flow along the first sample loading channel 103, and the outer surface of the sample loading pool 101 is provided with scale lines 1011. In this way, the scale lines 1011 can directly reflect the volume of the reaction liquid added in the sample loading pool 101, reducing the dependence on external sample loading equipment due to the need to accurately control the amount of reaction liquid added. The scale lines 1011 set in the sample loading pool 101 directly reflect the volume of the added reaction liquid, further improving the convenience of the nucleic acid detection process and further improving the detection efficiency.

[0042] like Figure 2 and Figure 5As shown, a first threaded structure 1012 is provided on the outside of the sample loading pool 101; the sealing cover 200 includes a cap 201 and a plunger 202, and a second threaded structure 203 is provided on the inner side of the cap 201, and the second threaded structure 203 is threadedly matched with the first threaded structure 1012, so that the cap 201 can be covered with the sample loading pool 101; the plunger 202 is fixedly connected to the inside of the cap 201 and protrudes from the cap 201, and the plunger 202 is sealed with the sample loading pool 101, so that when the cap 201 is screwed on the sample loading pool 101, the plunger 202 gradually compresses the air at the top of the sample loading pool 101, thereby providing pressure to the reaction liquid in the sample loading pool 101, so that the reaction liquid can move along the first injection channel 103.

[0043] In some embodiments, in order to ensure a sealed connection between the plunger 202 and the sample reservoir 101 , the plunger 202 is made of an elastic material, such as a rubber material or a silicone material, to ensure a sealed connection between the plunger 202 and the sample reservoir 101 .

[0044] like Figure 3 As shown, the sample loading pool 101 includes a connected equal-diameter section and a tapered section. The tapered section gradually tapers from the equal-diameter section to the injection hole, and the equal-diameter section and the tapered section are distributed in sequence along the first direction, so that the sample loading pool 101 forms a funnel structure. Under the pressure of the plunger 202, the reaction liquid can flow more smoothly in the sample loading pool 101, reducing the residual reaction liquid in the sample loading pool 101, thereby improving the accuracy of the reaction process.

[0045] like Figure 2 As shown, the first injection channel 103 is connected to at least two reaction units, and the at least two reaction units are distributed sequentially along the direction of flow of the reaction liquid in the second direction, so that the reaction liquid can perform nucleic acid amplification reaction in the reaction unit to achieve nucleic acid detection.

[0046] It should be noted that the first direction and the second direction may form an acute angle, a right angle, or an obtuse angle. In the embodiment of the present application, in order to ensure the filling effect of the reaction liquid on each reaction unit, the first direction and the second direction are arranged perpendicularly.

[0047] In some embodiments, as Figure 2 As shown, there are three reaction units, and the three reaction units are distributed in sequence along the direction of flow of the reaction liquid in the second direction; of course, the reaction units can also be four, five, etc., which can be set according to actual needs, and the embodiment of the present application does not limit this.

[0048] like Figure 2As shown, the reaction unit includes a second injection channel 104, a reaction chamber 105, a first exhaust channel 106 and a first exhaust hole 107; wherein, the second injection channel 104 is connected to the first injection channel 103, and the second injection channel 104 extends along the first direction, and the flow cross-section of the second injection channel 104 is larger than the flow cross-section of the first injection channel 103; the reaction chamber 105 is connected to the second injection channel 104, and the reaction chamber 105 is connected to the first exhaust hole 107 through the first exhaust channel 106, so that the reaction liquid can preferentially fill the second injection channel 104 and the reaction chamber 105.

[0049] like Figure 2 As shown, in the three reaction units distributed along the second direction, the apertures of the first exhaust holes 107 decrease in sequence along the direction of reaction liquid flow, so that the reaction chambers 105 along the direction of reaction liquid flow can be filled in sequence, thereby ensuring that the reaction liquid can be evenly distributed in each reaction chamber 105 in the cartridge body 100. Figure 2 As shown, a waste liquid pool 108 is connected to the end of the first injection channel 103, and the waste liquid pool 108 is connected to a second exhaust hole 110 through a second exhaust channel 109. The aperture of the second exhaust hole 110 is smaller than the aperture of the first exhaust hole 107, so that after the reaction liquid fills all the reaction chambers 105 in sequence, the remaining waste liquid will enter the waste liquid pool 108, so as to ensure that there is enough reaction liquid in the reaction chamber 105 for reaction, thereby further improving the accuracy of nucleic acid detection.

[0050] like Figure 1 、 Figure 3 and Figure 4 As shown, the cartridge body 100 includes a first surface and a second surface that are relatively distributed; on the first surface, the injection hole 102, the first injection channel 103, the second injection channel 104, the reaction chamber 105, the first exhaust channel 106, and the second exhaust channel 109 are all open structures and are all open to the first surface and form a reaction area;

[0051] The first exhaust holes 107 and the second exhaust holes 110 penetrate and connect the first surface and the second surface. A film pasting area 111 is formed on the second surface. The film pasting area 111 covers the first exhaust holes 107 and the second exhaust holes 110 .

[0052] The reaction area is sealed by the first seal 300, and the first seal 300 can be sealed to the reaction area of ​​the card box body 100 by bonding, heat sealing, hot pressing welding, ultrasonic welding, laser welding, etc., which is not limited in this embodiment of the present application.

[0053] In some other embodiments, the first sealing member 300 may be selected to be a sealing member with one-sided adhesive tape, so that the first sealing member 300 can directly seal the reaction area to improve detection efficiency.

[0054] In order to ensure the exhaust operation of the first exhaust hole 107 and the second exhaust hole 110, the second seal 400 is a hydrophobic breathable membrane, and the hydrophobic breathable membrane is sealed in the film area 111. The second seal 400 can be sealed in the film area 111 of the card box body 100 by bonding, heat sealing, hot pressure welding, ultrasonic welding, laser welding, etc., and the embodiment of the present application does not limit this.

[0055] In some other embodiments, the second sealing member 400 may be selected to be a sealing member with one-sided adhesive tape, so that the second sealing member 400 can directly seal the film-applying area 111 , thereby improving detection efficiency.

[0056] It should be noted that the hydrophobic and breathable membrane can be made of hydrophobic and breathable materials such as polytetrafluoroethylene, polyvinylidene fluoride, nanofiber membrane and graphene-based materials to maintain the air pressure balance between the inside of the card box body 100 and the outside world, reduce the leakage of reaction liquid due to the increase in air pressure inside the card box body 100 during nucleic acid amplification, and further ensure the accuracy and biosafety of the detection process.

[0057] like Figure 2 、 Figure 6 and Figure 7 As shown, in order to facilitate the insertion of the cartridge body 100 and the reaction instrument 500, the reaction instrument 500 is provided with a slot 501, and the cartridge body 100 is inserted into the slot 501;

[0058] The cartridge body 100 is provided with first positioning portions 120 at both ends in the second direction. The first positioning portions 120 are used to position the insertion depth of the cartridge body 100 in the reaction instrument 500.

[0059] A second positioning portion 130 is provided at at least one end of the cartridge body 100 in the second direction, the second positioning portion 130 extends along the first direction, and the reactor 500 is provided with at least one positioning groove 502, the second positioning portion 130 is used to position and cooperate with the positioning groove 502, so that in the process of inserting the cartridge body 100 into the interior of the reactor 500, the second positioning portion 130 is used to position and cooperate with the positioning groove 502 so that the cartridge body 100 can be correctly inserted, and the insertion depth of the cartridge body 100 is positioned by the first positioning portion 120 to ensure that the cartridge body 100 can perform a stable reaction in the reactor 500, thereby reducing the phenomenon of improper insertion between the cartridge body 100 and the reactor 500 and improving the accuracy of detection.

[0060] To ensure detection, at least one of the reactor 500 or the first seal 300 is entirely or partially made of a light-transmitting material, so that during the detection process, the fluorescence can pass through the light-transmitting material to achieve signal collection. In some embodiments, the light-transmitting material can be polycarbonate, polymethyl methacrylate, polypropylene, polyethylene terephthalate, polystyrene, polyethylene, polyamide, polyvinyl chloride, etc., which is not limited in this embodiment of the present application.

[0061] During use of the nucleic acid amplification reaction cartridge provided in the embodiment of the present application, the first sealing member 300 is firstly sealed with the reaction area of ​​the cartridge body 100, and the second sealing member 400 is sealed with the film-applying area 111; then, the reaction liquid is added to the sample loading reservoir 101, and the volume of the added reaction liquid is observed through the scale line 1011; then, the sealing cover 200 is screwed onto the sample loading reservoir 101, and under the pressure of the plunger 202, the reaction liquid flows along the first injection channel 103, and the reaction liquid fills the reaction chamber 105 in sequence along the flow direction, and the remaining waste liquid enters the waste liquid reservoir 108 along the first injection channel 103; then, the sealed cartridge body 100 is inserted into the interior of the reactor 500 along the card slot 501 to perform nucleic acid amplification reaction and detection, thereby improving the convenience of nucleic acid detection and improving the efficiency of nucleic acid detection.

[0062] In another use process of the nucleic acid amplification reaction cartridge provided in an embodiment of the present application, the enzymes, primers, probes and other biological reagents required for the nucleic acid amplification reaction are first pre-placed in the reaction chamber 105; then the first seal 300 is used to seal the reaction area of ​​the cartridge body 100, and the second seal 400 is used to seal the film area 111; then the sample liquid is added to the sample loading reservoir 101, and then the sealing cover 200 is screwed onto the sample loading reservoir 101. Under the pressure of the plunger 202, the sample liquid flows along the first injection channel 103 and fills the reaction chamber 105 in the flow direction, dissolving the pre-placed biological reagents in the reaction chamber 105, simplifying the reaction liquid configuration process and further improving the portability of the operation; then the sealed cartridge body 100 is inserted into the interior of the reactor 500 along the card slot 501 to perform the nucleic acid amplification reaction and detection. The above description of the disclosed embodiments enables those skilled in the art to implement or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A nucleic acid amplification reaction cartridge, for being inserted into a reactor (500), characterized in that: include: Card box body (100) and sealing cover (200); Wherein, the sealing cover (200) is used to seal the card box body (100); A sample loading reservoir (101) is provided on the top of the cartridge body (100), the sample loading reservoir (101) is connected to a first sample injection channel (103), and the sealing cover (200) is used to seal the sample loading reservoir (101); Furthermore, in the process of the sealing cover (200) sealing the sample loading pool (101), the reaction liquid in the sample loading pool (101) can be driven to flow along the first sample loading channel (103).

2. The nucleic acid amplification reaction cartridge according to claim 1, wherein The sample addition pool (101) is connected to the first sample injection channel (103) through the injection hole (102), and the flow cross section of the first sample injection channel (103) is much smaller than the cross section of the sample addition pool (101); The outer surface of the sample addition pool (101) is provided with scale lines (1011).

3. The nucleic acid amplification reaction cartridge according to claim 1, wherein The outside of the sample addition pool (101) is provided with a first thread structure (1012); The sealing cover (200) comprises: A cover cap (201), wherein a second thread structure (203) is provided on the inner side of the cover cap (201), and the second thread structure (203) is threadably engaged with the first thread structure (1012); A plunger (202) is fixedly connected to the interior of the cap (201) and protrudes from the cap (201), and the plunger (202) is sealed and matched with the sample addition pool (101).

4. The nucleic acid amplification reaction cartridge according to claim 3, wherein The plunger (202) is made of rubber; alternatively, the plunger (202) is made of silicone.

5. The nucleic acid amplification reaction cartridge according to claim 2, wherein: The sample addition pool (101) comprises a connected equal diameter section and a tapered section, wherein the tapered section gradually tapers from the equal diameter section toward the sample injection hole (102); The constant diameter sections and the tapered sections are distributed in sequence along the first direction.

6. The nucleic acid amplification reaction cartridge according to claim 2, wherein: The first injection channel (103) is connected to at least two reaction units; At least two reaction units are distributed sequentially in the second direction along the flow direction of the reaction liquid; An angle is formed between the first direction and the second direction.

7. The nucleic acid amplification reaction cartridge according to claim 6, characterized in that: The reaction unit comprises: a second injection channel (104), wherein the second injection channel (104) is connected to the first injection channel (103), the second injection channel (104) extends along a first direction, and a flow cross-section of the second injection channel (104) is larger than a flow cross-section of the first injection channel (103); a reaction chamber (105), the reaction chamber (105) being connected to the second injection channel (104); a first exhaust hole (107), the first exhaust hole (107) being connected to the reaction chamber (105) via a first exhaust channel (106); The first exhaust hole (107), the first exhaust channel (106), the reaction chamber (105) and the second injection channel (104) are distributed in sequence along a first direction.

8. The nucleic acid amplification reaction cartridge according to claim 7, wherein: In at least two of the reaction units, the apertures of the first exhaust holes (107) decrease in sequence along the direction of reaction liquid flow.

9. The nucleic acid amplification reaction cartridge according to claim 7, wherein: The end of the first injection channel (103) is connected to a waste liquid pool (108); The waste liquid pool (108) is connected to the second exhaust hole (110) through a second exhaust channel (109), and the aperture of the second exhaust hole (110) is smaller than the aperture of the first exhaust hole (107).

10. The nucleic acid amplification reaction cartridge according to claim 9, characterized in that: The card box body (100) comprises a first surface and a second surface that are oppositely distributed; On the first surface, the injection hole (102), the first injection channel (103), the second injection channel (104), the reaction chamber (105), the first exhaust channel (106), the waste liquid pool (108), and the second exhaust channel (109) are all open structures and are all open facing the first surface to form a reaction zone; The first exhaust hole (107) and the second exhaust hole (110) penetrate and connect the first surface and the second surface, and the second surface forms a film-sticking area (111), which covers the first exhaust hole (107) and the second exhaust hole (110).

11. The nucleic acid amplification reaction cartridge according to claim 10, wherein: The reaction zone is sealed by a first seal (300); The film-sticking area (111) is sealed by a second sealing member (400).

12. The nucleic acid amplification reaction cartridge according to claim 11, wherein The second sealing member (400) is a hydrophobic breathable membrane.

13. The nucleic acid amplification reaction cartridge according to any one of claims 1 to 12, characterized in that: The reaction instrument (500) is provided with a card slot (501), and the card box body (100) is used for plugging and matching with the card slot (501); Both ends of the cartridge body (100) in the second direction are provided with first positioning portions (120), and the first positioning portions (120) are used to position the insertion depth of the cartridge body (100) in the reaction instrument (500); The cartridge body (100) is provided with a second positioning portion (130) at at least one end in the second direction, and the second positioning portion (130) extends along the first direction. The reactor (500) is provided with at least one positioning groove (502), and the second positioning portion (130) is used for positioning and cooperating with the positioning groove (502).

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