Culture container conveying device and method for cell culture

Abstract

The invention discloses a culture container conveying device and method for cell culture, and belongs to the technical field of cell culture.The culture container conveying device is technically characterized by comprising an incubator and a second supporting seat, culture containers can be limited one by one through an arranged limiting conveying mechanism, meanwhile, automation of the culture container conveying and material taking process is achieved, and the production efficiency is improved. In addition, when the culture container is not grabbed by the material, the culture box is always in a closed state, a clean environment is provided for cell culture, the risk of experiment failure caused by pollution is reduced, automatic opening, closing and positioning of a plurality of limiting rings are realized through an automatic opening, closing and positioning operation mode of the positioning observation mechanism, and the operation efficiency is improved. The device is simple in structure and convenient to operate, does not need manual operation, greatly shortens the positioning time of the culture container, avoids frequent contact of operators with the culture container, reduces the risk that external pollutants are attached to the surface of the culture container, and has the advantages of convenience in closed transmission, automatic material taking and automatic positioning, observation and use.

Description

Technical Field

[0001] The present invention relates to the field of cell culture, and in particular to a culture container transmission device and method for cell culture. Background Art

[0002] Cell culture is a key technology in modern biomedical research, cell therapy, and biopharmaceuticals. Cells are extremely sensitive to their culture environment, requiring strict control of factors such as temperature, humidity, and sterility. Cell culture involves mimicking the in vivo environment (sterility, optimal temperature, pH, and specific nutritional conditions) in vitro to enable cells to survive, grow, reproduce, and maintain their primary structures and functions.

[0003] Currently, the transportation of culture containers mostly relies on manual operation, which has many problems: on the one hand, manual transportation is inefficient and cannot meet the needs of large-scale cell culture; on the other hand, before the observation test, the culture container needs to be placed in a relatively closed space for certain temperature and humidity monitoring. Manual removal requires manually opening the door to take it and then manually closing the door. This manual operation can easily cause external pollutants to enter the culture container, causing cell contamination and reducing the success rate of cell culture. In addition, when observing cell proliferation and cell morphology, the position of the culture container needs to be manually corrected to facilitate subsequent observation with a microscope. However, manual operation is inefficient and cannot meet the needs of actual use.

[0004] Therefore, it is necessary to provide a culture container transfer device and method for cell culture, aiming to solve the above problems. Summary of the Invention

[0005] In view of the shortcomings of the prior art, the embodiment of the present invention aims to provide a culture container transfer device and method for cell culture, aiming to solve the technical problems raised in the background art.

[0006] To achieve the above object, the present invention provides the following technical solutions: A culture container transport device for cell culture, comprising an incubator and a second support base, wherein an electric door for placing the culture container is provided on the outer side of the incubator, and a microscope for observation is mounted on the second support base via a vertical plate, and further comprising: A position limiting and transporting mechanism, installed inside the incubator, for transporting the culture containers one by one, the position limiting and transporting mechanism comprising a T-shaped position limiting plate for limiting the position of the culture containers one by one, the T-shaped position limiting plate being provided with a first position limiting wheel and a second position limiting wheel for guiding and limiting the movement of the culture containers; A material taking and placing mechanism is installed on the outside of the incubator and is used to take out the culture container for placement and observation. The material taking and placing mechanism includes a second sliding door and a first sliding door for sealing the incubator and connecting the material taking. The first sliding door is slidingly connected to one side of the second sliding door. A clamping plate for taking out the material is provided on one side of the first sliding door. A first fixed frame plate is connected to one side of the first sliding door and the second sliding door. The first fixed frame plate is fixedly installed inside the incubator. A second guide groove is opened on the first fixed frame plate for driving the clamping plate to turn the material taking. A positioning and observation mechanism is installed on the second support seat and is driven to open and center by the material taking and placing mechanism, and is used to position and center the placed culture container. The positioning and observation mechanism includes a first L-shaped connecting rod, a second L-shaped connecting rod and a third L-shaped connecting rod for centering drive. The first L-shaped connecting rod, the second L-shaped connecting rod and the third L-shaped connecting rod are all movably installed on the second support seat through a driving plate.

[0007] As a further solution of the present invention, the position limiting transmission mechanism also includes a first conveying platform, a second conveying platform and a third conveying platform for conveying culture containers. The first conveying platform, the second conveying platform and the third conveying platform are docked and connected one by one. The third conveying platform is provided with a position limiting rod for limiting a single conveyed culture container. The first conveying platform, the second conveying platform and the third conveying platform are all installed inside the incubator through the first support seat.

[0008] As a further solution of the present invention, the limiting transmission mechanism also includes a first electric push rod for driving the T-shaped limiting plate for limiting transmission, the T-shaped limiting plate is rotatably installed on one side of the third conveying platform through a first connecting shaft, one end of the T-shaped limiting plate is rotatably connected to the docking plate through a second connecting shaft, the docking plate is fixedly connected to the piston rod of the first electric push rod, and the first electric push rod is rotatably installed on the first conveying platform through a third connecting shaft.

[0009] As a further solution of the present invention, the material picking and placing mechanism also includes a second electric push rod and a fixed frame for clamping the culture container for material picking and placing connection drive, the clamping plate is rotatably connected to the extension plate through a fourth connecting shaft, the extension plate is fixedly connected to the piston rod of the second electric push rod, the second electric push rod is fixedly installed on the sealing plate through the fixed frame, the sealing plate is fixedly installed on one side of the first sliding door, one end of the clamping plate is rotatably connected to the docking rod, the other end of the docking rod is hingedly installed on the hinge seat, the hinge seat is fixedly installed on the fixed frame, and a push plate is also provided on the extension plate.

[0010] As a further solution of the present invention, the material picking and placing mechanism also includes a multi-stage electric telescopic rod and a motor for driving the splint to move and turn the connection. The first sliding door is slidingly connected to the second guide groove on the rotating plate and the first fixed frame plate through the second slider. The second slider is movably connected to the piston rod of the multi-stage electric telescopic rod. The multi-stage electric telescopic rod is rotatably installed on the first fixed frame plate through the rotating block. The rotating plate is provided with a first guide groove for adapting the sliding connection of the second slider. The rotating plate is fixedly connected to the output shaft of the motor. The motor is fixedly installed inside the incubator through the fixed block. The second guide groove is provided with a first area and a second area. The second sliding door is fixedly installed on the rotating plate.

[0011] As a further solution of the present invention, the positioning and observation mechanism also includes a first limiting ring, a second limiting ring and a third limiting ring for positioning the culture container for centering processing, the first limiting ring is fixedly connected to one end of the first L-shaped connecting rod, the second limiting ring is fixedly connected to one end of the second L-shaped connecting rod, the third limiting ring is fixedly connected to one end of the third L-shaped connecting rod, the first L-shaped connecting rod is rotatably connected to the second support seat through the sixth connecting shaft, and the second L-shaped connecting rod and the third L-shaped connecting rod are both rotatably connected to the second support seat through the seventh connecting shaft.

[0012] As a further solution of the present invention, the positioning and observation mechanism also includes a driving plate and a sliding rod for driving the first limiting ring, the second limiting ring and the third limiting ring to be connected to each other, the driving plate is connected to the second fixed frame plate by the sliding rod, the second fixed frame plate is fixedly installed on the second support seat, the first L-shaped connecting rod is connected to the driving plate by the first slider, the second L-shaped connecting rod is connected to the driving plate by the third slider, and the third L-shaped connecting rod is connected to the driving plate by the fourth slider.

[0013] As a further solution of the present invention, the positioning and observation mechanism also includes an L-shaped tooth plate and a sliding tooth plate for driving the driving plate to perform a movement control connection, the L-shaped tooth plate is fixedly connected to one end of the sliding rod, and the L-shaped tooth plate and the sliding tooth plate are both limitedly slidably connected to the inside of the second support seat, and the L-shaped tooth plate and the sliding tooth plate are both meshed and connected with a rotating gear, and the rotating gear is rotatably connected to the inside of the second support seat through a fifth connecting shaft, and a return spring is provided at the internal connection between one end of the sliding tooth plate and the second support seat, and a pressing plate is provided at the other end of the sliding tooth plate, and one side of the pressing plate is adapted to be connected with a push plate.

[0014] A method for using a culture container transfer device for cell culture, comprising the following steps: Step 1: Open the electric door of the incubator and place the culture container to be cultured on the first conveyor platform for transportation. After the first, second, and third conveyor platforms are connected one by one, multiple culture containers are stacked on one side of the T-shaped limit plate on the third conveyor platform. The first limit wheel on the T-shaped limit plate limits the culture container. Step 2: When the culture container needs to be transported out individually for material removal, the first electric push rod drives the T-shaped limit plate to rotate in the connection relationship with the first connecting shaft. At this time, the first electric push rod is in a retracted state, so the first limit wheel on the T-shaped limit plate is away from the limit of the culture container, and the second limit wheel on the T-shaped limit plate can push the culture container at this location to the side close to the limit rod. Then the first electric push rod is reset and extended. At this time, the T-shaped limit plate is reset to its original position to limit the next culture container; Step 3: The multi-stage electric telescopic rod drives the first sliding door and the splint connected to the second slider to retract and move, and then retract under the guidance of the second guide groove on the first fixed frame plate. When the multi-stage electric telescopic rod reaches the curved position close to the second guide groove, it stops retracting and the motor drives the rotating plate and the splint on the first sliding door to rotate 90 degrees. At this time, the second slider moves from the first area to the second area. Step 4: When the second slider moves to the second area, the clamping plate moves into the clamping range of the culture container on the third conveyor platform. The second electric push rod drives the extension plate to retract and move. At this time, under the connection relationship of the docking link, the clamping plates on the extension plate are close to each other to complete the clamping and fixing operation of the culture container. Then the motor resets and rotates in the opposite direction, the multi-stage electric telescopic rod extends and moves, and the clamping plate moves the clamped culture container to the second support seat for placement; When the second slider is pushed forward by the multi-stage electric telescopic rod under the guidance of the second guide groove, the pushing plate on the extension plate can push the sliding tooth plate on the pressing plate to move closer to the inside of the second support seat. Under the connection relationship of the rotating gear, the L-shaped tooth plate drives the driving plate on the sliding rod to move in the direction away from the second fixed frame plate. Under the traction movement of the first slider on the first L-shaped connecting rod, the third slider on the second L-shaped connecting rod and the fourth slider on the third L-shaped connecting rod, the first limiting ring on the first L-shaped connecting rod, the second limiting ring on the second L-shaped connecting rod and the third limiting ring on the third L-shaped connecting rod are moved away from each other, which facilitates the placement of the culture container clamped by the splint. Step 6: When the extension plate retracts after the culture container is placed, the sliding tooth plate moves toward the outside of the second support seat under the reset action of the reset spring. Then, under the connection relationship of the rotating gear, the L-shaped tooth plate drives the driving plate on the sliding rod to move toward the second fixed frame plate. Under the traction and movement of the first slider on the first L-shaped connecting rod, the third slider on the second L-shaped connecting rod and the fourth slider on the third L-shaped connecting rod, the first limiting ring on the first L-shaped connecting rod, the second limiting ring on the second L-shaped connecting rod and the third limiting ring on the third L-shaped connecting rod are brought close to each other, which is convenient for centering and positioning the placed culture container, thereby facilitating the observation operation of the microscope above.

[0015] In summary, the embodiments of the present invention have the following beneficial effects compared with the prior art: The present invention provides a limiting transmission mechanism, which can limit the culture containers one by one, so that the culture containers remain stable during the subsequent material collection and transmission process, avoiding shaking and collision, effectively protecting the culture containers and the cells cultured therein, and improving the quality of cell culture. Moreover, since the culture containers are limited one by one, when subsequently collecting materials, the clamping clamp can accurately and quickly find the position of the culture container at that location, facilitating the collection operation, greatly improving the work efficiency of cell culture, reducing the risk of sample contamination and operational errors caused by human factors, ensuring the safety of cell culture experiments and the accuracy of experimental results, and realizing the automation of the culture container transmission and collection process, reducing manual intervention, improving the automation level of the entire cell culture process, reducing labor costs, and ensuring the consistency and stability of the operation. In addition, when the culture container is not being collected and grabbed, the incubator is always in a closed state, and impurities such as dust and microorganisms from the outside cannot enter the incubator, avoiding contamination of the culture container and cell samples, providing a clean environment for cell culture, ensuring the normal growth and culture quality of cells, and reducing the risk of experimental failure due to contamination.

[0016] Through the automatic opening and closing and positioning operation mode of the positioning observation mechanism, the automatic opening and closing and positioning of multiple limit rings are realized without manual operation, which greatly shortens the positioning time of the culture container. At the same time, the culture container can be accurately limited to the specified position of the observation platform, avoiding positioning deviation caused by human operation, and ensuring the reliability of the observation results. At the same time, this automatic positioning process reduces human intervention, reduces the possibility of errors introduced by operators due to improper manual operation, avoids frequent contact between operators and culture containers, reduces the risk of external contaminants adhering to the surface of culture containers, better ensures the cleanliness of the cell culture environment, and is conducive to the healthy growth of cells and the smooth progress of experiments.

[0017] In order to more clearly illustrate the structural features and effects of the present invention, the present invention is described in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of an embodiment of the invention.

[0019] Figure 2 It is a side structural schematic diagram of an embodiment of the invention.

[0020] Figure 3 Schematic diagram of the connection structure inside the incubator in an embodiment of the invention.

[0021] Figure 4 This is a side view schematic diagram of the internal connections of the incubator in an embodiment of the invention.

[0022] Figure 5 for Figure 4 Schematic diagram of the enlarged structure of A in the figure.

[0023] Figure 6 Schematic diagram of the connection structure of the first fixed frame plate in an embodiment of the invention.

[0024] Figure 7 for Figure 6 Schematic diagram of the enlarged structure of B.

[0025] Figure 8 Schematic diagram of the connection structure of the first sliding door in an embodiment of the invention.

[0026] Figure 9 Schematic diagram of the connection structure inside the second support seat in an embodiment of the invention.

[0027] Figure 10 for Figure 9 Schematic diagram of the enlarged structure of C in the middle.

[0028] Figure 11 Schematic diagram of the connection structure of the driving plate in an embodiment of the invention.

[0029] Figure numerals: 1, incubator; 2, electric door; 3, first support seat; 4, first conveyor platform; 5, second conveyor platform; 6, third conveyor platform; 7, culture container; 8, limiting rod; 9, T-shaped limiting plate; 10, first limiting wheel; 11, second limiting wheel; 12, first connecting shaft; 13, first slider; 14, second connecting shaft; 15, docking plate; 16, first electric push rod; 17, third connecting shaft; 18, splint; 19, extension plate; 20, fourth connecting shaft; 21, docking link; 22, hinged seat; 23, second electric push rod; 24, fixed frame; 25, sealing plate; 26, first sliding door; 27, second sliding door; 28, rotating plate; 29, first guide groove; 30, second slider; 31, first fixed frame Plate; 32. Second guide groove; 33. Multi-stage electric telescopic rod; 34. Rotating block; 35. Fixed block; 36. Motor; 37. First area; 38. Second area; 39. Push plate; 40. Pressing plate; 41. Sliding tooth plate; 42. Return spring; 43. Rotating gear; 44. Fifth connecting shaft; 45. L-shaped tooth plate; 46. Sliding rod; 47. Second fixed frame plate; 48. Driving plate; 49. First L-shaped connecting rod; 50. Sixth connecting shaft; 51. First limiting ring; 52. Seventh connecting shaft; 53. Second L-shaped connecting rod; 54. Third slider; 55. Second limiting ring; 56. Third L-shaped connecting rod; 57. Third limiting ring; 58. Fourth slider; 59. Vertical plate; 60. Microscope; 61. Second support seat. DETAILED DESCRIPTION

[0030] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0031] The specific implementation of the present invention is described in detail below with reference to specific embodiments. Example 1

[0032] See also Figures 1 to 9 A culture container transfer device for cell culture includes an incubator 1 and a second support base 61. An electric door 2 for placing a culture container 7 is provided on the outside of the incubator 1. A microscope 60 for observation is mounted on the second support base 61 via a vertical plate 59. The device also includes: A position limiting and transporting mechanism is installed inside the incubator 1 and is used to transport the culture containers 7 one by one. The position limiting and transporting mechanism includes a T-shaped position limiting plate 9 for limiting the position of the culture containers 7 one by one during transport. The T-shaped position limiting plate 9 is provided with a first position limiting wheel 10 and a second position limiting wheel 11 for guiding and limiting the movement of the culture containers 7. The material taking and placing mechanism is installed on the outside of the incubator 1 and is used to take out the culture container 7 for placement and observation. The material taking and placing mechanism includes a second sliding door 27 and a first sliding door 26 for sealing the incubator 1 and connecting the material taking. The first sliding door 26 is limitedly slidably connected to one side of the second sliding door 27. A splint 18 for taking materials is provided on one side of the first sliding door 26. One side of the first sliding door 26 and the second sliding door 27 are connected to a first fixed frame plate 31. The first fixed frame plate 31 is fixedly installed inside the incubator 1. A second guide groove 32 is provided on the first fixed frame plate 31 for driving the splint 18 to turn the material taking.

[0033] Furthermore, the position-limiting transmission mechanism also includes a first conveying platform 4, a second conveying platform 5 and a third conveying platform 6 for conveying the culture container 7. The first conveying platform 4, the second conveying platform 5 and the third conveying platform 6 are docked and connected one by one. The third conveying platform 6 is provided with a position-limiting rod 8 for limiting the position of a single conveyed culture container 7. The first conveying platform 4, the second conveying platform 5 and the third conveying platform 6 are all installed inside the incubator 1 through the first support seat 3.

[0034] Furthermore, the limiting transmission mechanism also includes a first electric push rod 16 for driving the T-shaped limiting plate 9 to perform limiting transmission. The T-shaped limiting plate 9 is rotatably installed on one side of the third conveying platform 6 through the first connecting shaft 12. One end of the T-shaped limiting plate 9 is rotatably connected to the docking plate 15 through the second connecting shaft 14. The docking plate 15 is fixedly connected to the piston rod of the first electric push rod 16. The first electric push rod 16 is rotatably installed on the first conveying platform 4 through the third connecting shaft 17.

[0035] Furthermore, the material taking and placing mechanism also includes a second electric push rod 23 and a fixed frame 24 for clamping the culture container 7 for material taking and placing connection drive. The clamping plate 18 is rotatably connected to the extension plate 19 through the fourth connecting shaft 20. The extension plate 19 is fixedly connected to the piston rod of the second electric push rod 23. The second electric push rod 23 is fixedly installed on the sealing plate 25 through the fixed frame 24. The sealing plate 25 is fixedly installed on one side of the first sliding door 26. One end of the clamping plate 18 is rotatably connected to the docking link 21. The other end of the docking link 21 is hingedly installed on the hinge seat 22. The hinge seat 22 is fixedly installed on the fixed frame 24. A push plate 39 is also provided on the extension plate 19.

[0036] Furthermore, the material picking and placing mechanism also includes a multi-stage electric telescopic rod 33 and a motor 36 for driving the splint 18 to move and turn. The first sliding door 26 is slidingly connected to the rotating plate 28 and the second guide groove 32 on the first fixed frame plate 31 through the second slider 30. The second slider 30 is movably connected to the piston rod of the multi-stage electric telescopic rod 33. The multi-stage electric telescopic rod 33 is rotatably installed on the first fixed frame plate 31 through the rotating block 34. The rotating plate 28 is provided with a first guide groove 29 for adapting the sliding connection with the second slider 30. The rotating plate 28 is fixedly connected to the output shaft of the motor 36. The motor 36 is fixedly installed inside the incubator 1 through the fixed block 35. The second guide groove 32 is provided with a first area 37 and a second area 38. The second sliding door 27 is fixedly installed on the rotating plate 28.

[0037] Preferably, the electric door 2 on the incubator 1 is opened, and the culture container 7 that needs to be cultured for cells is placed on the first conveyor platform 4 for transportation. After the first conveyor platform 4, the second conveyor platform 5 and the third conveyor platform 6 are connected one by one, multiple culture containers 7 are stacked on one side of the T-shaped limiting plate 9 on the third conveyor platform 6, and the first limiting wheel 10 on the T-shaped limiting plate 9 limits the culture container 7, thereby facilitating subsequent material collection processing.

[0038] When the culture container 7 needs to be transported out individually for material collection, the first electric push rod 16 drives the T-shaped limit plate 9 to rotate under the connection relationship with the first connecting shaft 12. At this time, the first electric push rod 16 is in a retracted state, so the first limit wheel 10 on the T-shaped limit plate 9 is away from the limit of the culture container 7. The second limit wheel 11 on the T-shaped limit plate 9 can push the culture container 7 at this location to the side close to the limit rod 8. Then the first electric push rod 16 is reset and extended. At this time, the T-shaped limit plate 9 is reset to its original position to perform the limit processing of the next culture container 7.

[0039] By setting a limiting transmission mechanism, the culture container 7 can be limited one by one, so that the culture container 7 remains stable during the subsequent material collection and transmission process, avoiding shaking and collision, effectively protecting the culture container 7 and the cells cultured therein, and improving the quality of cell culture. In addition, since the culture container 7 is limited one by one, when the material is subsequently collected, the clamping splint 18 can accurately and quickly find the position of the culture container 7, which is convenient for the material collection operation and greatly improves the work efficiency of cell culture.

[0040] When the material is taken out, the multi-stage electric telescopic rod 33 drives the first sliding door 26 and the clamping plate 18 connected to the second slider 30 to retract and move, and moves back under the guidance of the second guide groove 32 on the first fixed frame plate 31. When the multi-stage electric telescopic rod 33 reaches the curved position close to the second guide groove 32, it stops retracting and moves. The motor 36 drives the rotating plate 28 and the clamping plate 18 on the first sliding door 26 to rotate 90 degrees. That is, at this time, the second slider 30 moves from the first area 37 to the second area 38. When the second slider 30 moves to the second area 38, the clamping plate 18 moves to the clamping range of the culture container 7 on the third conveyor 6. The second electric push rod 23 drives the extension plate 19 to retract and move. At this time, under the connection relationship of the docking link 21, the clamping plates 18 on the extension plate 19 are close to each other to complete the clamping and fixing operation of the culture container 7. Then the motor 36 resets and rotates in the opposite direction, the multi-stage electric telescopic rod 33 extends and moves, and the clamping plate 18 moves the clamped culture container 7 to the second support seat 61 for placement.

[0041] This method of grabbing the culture container 7 by the splint 18 and rotating it 90° can make the specific structural direction of the culture container 7 compatible with the requirements of subsequent experimental equipment. There is no need for manual adjustment by the operator, which reduces the number of operating steps, improves experimental efficiency, reduces the risk of sample contamination and operational errors caused by human factors, ensures the safety of cell culture experiments and the accuracy of experimental results, and at the same time realizes the automation of the culture container 7 transmission and material collection process, reduces manual intervention, improves the automation level of the entire cell culture process, reduces labor costs, and also ensures the consistency and stability of operations. In addition, when the culture container 7 is not grabbed for material collection, the incubator 1 is always in a closed state, and external dust, microorganisms and other impurities cannot enter the interior of the incubator 1, avoiding contamination of the culture container 7 and cell samples, providing a clean environment for cell culture, ensuring the normal growth and culture quality of cells, and reducing the risk of experimental failure due to contamination. Example 2

[0042] like Figures 1 to 11 As shown, this embodiment, based on embodiment 1, further includes a positioning observation mechanism, which is installed on the second support seat 61 and is driven to open and center by the material placement mechanism for positioning and centering the placed culture container 7. The positioning observation mechanism includes a first L-shaped connecting rod 49, a second L-shaped connecting rod 53 and a third L-shaped connecting rod 56 for centering drive. The first L-shaped connecting rod 49, the second L-shaped connecting rod 53 and the third L-shaped connecting rod 56 are all movably installed on the second support seat 61 through the driving plate 48.

[0043] Furthermore, the positioning and observation mechanism also includes a first limiting ring 51, a second limiting ring 55 and a third limiting ring 57 for positioning the culture container 7 for centering processing. The first limiting ring 51 is fixedly connected to one end of the first L-shaped connecting rod 49, the second limiting ring 55 is fixedly connected to one end of the second L-shaped connecting rod 53, and the third limiting ring 57 is fixedly connected to one end of the third L-shaped connecting rod 56. The first L-shaped connecting rod 49 is rotatably connected to the second support seat 61 through the sixth connecting shaft 50, and the second L-shaped connecting rod 53 and the third L-shaped connecting rod 56 are both rotatably connected to the second support seat 61 through the seventh connecting shaft 52.

[0044] Furthermore, the positioning and observation mechanism also includes a driving plate 48 and a sliding rod 46 for driving the first limiting ring 51, the second limiting ring 55 and the third limiting ring 57 to be connected to each other. The driving plate 48 is slidingly connected to the second fixed frame plate 47 through the sliding rod 46. The second fixed frame plate 47 is fixedly installed on the second support seat 61. The first L-shaped connecting rod 49 is slidingly connected to the driving plate 48 through the first slider 13. The second L-shaped connecting rod 53 is slidingly connected to the driving plate 48 through the third slider 54. The third L-shaped connecting rod 56 is slidingly connected to the driving plate 48 through the fourth slider 58.

[0045] Furthermore, the positioning and observation mechanism also includes an L-shaped tooth plate 45 and a sliding tooth plate 41 for driving the driving plate 48 to perform a movement control connection. The L-shaped tooth plate 45 is fixedly connected to one end of the sliding rod 46. The L-shaped tooth plate 45 and the sliding tooth plate 41 are both limitedly slidably connected to the inside of the second support seat 61. The L-shaped tooth plate 45 and the sliding tooth plate 41 are both meshed with a rotating gear 43. The rotating gear 43 is rotatably connected to the inside of the second support seat 61 through a fifth connecting shaft 44. A reset spring 42 is provided at the internal connection between one end of the sliding tooth plate 41 and the second support seat 61. A pressing plate 40 is provided at the other end of the sliding tooth plate 41, and a push plate 39 is adapted to be connected to one side of the pressing plate 40.

[0046] Preferably, in this embodiment, when the multi-stage electric telescopic rod 33 pushes the second slide block 30 to move forward under the guidance of the second guide groove 32, the pushing plate 39 on the extension plate 19 can push the sliding tooth plate 41 on the pressing plate 40 to move closer to the inside of the second support seat 61. Under the connection relationship of the rotating gear 43, the L-shaped tooth plate 45 drives the driving plate 48 on the sliding rod 46 to move in the direction away from the second fixed frame plate 47. Under the traction movement of the first slider 13 on the first L-shaped link 49, the third slider 54 on the second L-shaped link 53 and the fourth slider 58 on the third L-shaped link 56, the first limiting ring 51 on the first L-shaped link 49, the second limiting ring 55 on the second L-shaped link 53 and the third limiting ring 57 on the third L-shaped link 56 are moved away from each other, which is convenient for the culture medium clamped by the splint 18. The container 7 is placed, and when the extension plate 19 retracts after the culture container 7 is placed, the sliding tooth plate 41 moves toward the outside of the second support seat 61 under the reset action of the reset spring 42. Then, under the connection relationship of the rotating gear 43, the L-shaped tooth plate 45 drives the driving plate 48 on the sliding rod 46 to move toward the direction close to the second fixed frame plate 47. Under the traction and movement of the first slider 13 on the first L-shaped link 49, the third slider 54 on the second L-shaped link 53 and the fourth slider 58 on the third L-shaped link 56, the first limiting ring 51 on the first L-shaped link 49, the second limiting ring 55 on the second L-shaped link 53 and the third limiting ring 57 on the third L-shaped link 56 are close to each other, which is convenient for centering and positioning the placed culture container 7, thereby facilitating the observation operation of the microscope 60 above.

[0047] Through the automatic opening and closing and positioning operation mode of the positioning observation mechanism, the automatic opening and closing and positioning of multiple limit rings are realized without manual operation, which greatly shortens the positioning time of the culture container 7. At the same time, the culture container 7 can be accurately limited to the specified position of the observation platform, avoiding positioning deviation caused by human operation, and ensuring the reliability of the observation results. At the same time, this automatic positioning process reduces human intervention, reduces the possibility of errors introduced by operators due to improper manual operation, avoids frequent contact between operators and culture container 7, reduces the risk of external pollutants adhering to the surface of culture container 7, better ensures the cleanliness of the cell culture environment, and is conducive to the healthy growth of cells and the smooth progress of experiments.

[0048] A method for using a culture container transfer device for cell culture, comprising the following steps: Step 1: Open the electric door 2 on the incubator 1, place the culture container 7 to be cultured on the first conveyor platform 4 for transportation, and after the first conveyor platform 4, the second conveyor platform 5 and the third conveyor platform 6 are connected one by one, multiple culture containers 7 are stacked on one side of the T-shaped limit plate 9 on the third conveyor platform 6, and the first limit wheel 10 on the T-shaped limit plate 9 limits the position of the culture container 7; Step 2: When the culture container 7 needs to be transported out individually for material removal, the first electric push rod 16 drives the T-shaped limit plate 9 to rotate under the connection relationship with the first connecting shaft 12. At this time, the first electric push rod 16 is in a retracted state, so the first limit wheel 10 on the T-shaped limit plate 9 is away from the limit of the culture container 7, and the second limit wheel 11 on the T-shaped limit plate 9 can push the culture container 7 at this location to the side close to the limit rod 8. Then the first electric push rod 16 is reset and extended. At this time, the T-shaped limit plate 9 is reset to its original position to perform the limit processing of the next culture container 7; Step 3: The multi-stage electric telescopic rod 33 drives the first sliding door 26 and the clamping plate 18 connected to the second slider 30 to retract and move. The second slider 30 then moves back under the guidance of the second guide groove 32 on the first fixed frame plate 31. When the multi-stage electric telescopic rod 33 reaches the curved position close to the second guide groove 32, it stops retracting and the motor 36 drives the rotating plate 28 and the clamping plate 18 on the first sliding door 26 to rotate 90 degrees. At this time, the second slider 30 moves from the first area 37 to the second area 38. Step 4: When the second slide 30 moves to the second area 38, the clamping plate 18 moves into the clamping range of the culture container 7 on the third conveyor 6. The second electric push rod 23 drives the extension plate 19 to retract and move. At this time, under the connection relationship of the docking link 21, the clamping plates 18 on the extension plate 19 are close to each other to complete the clamping and fixing operation of the culture container 7. Then the motor 36 resets and rotates in the opposite direction, the multi-stage electric telescopic rod 33 extends and moves, and the clamping plate 18 moves the clamped culture container 7 to the second support seat 61 for placement; Step 5: When the multi-stage electric telescopic rod 33 pushes the second slide block 30 to move forward under the guidance of the second guide groove 32, the pushing plate 39 on the extension plate 19 can push the sliding tooth plate 41 on the pressing plate 40 to move closer to the inside of the second support seat 61. Under the connection relationship of the rotating gear 43, the L-shaped tooth plate 45 drives the driving plate 48 on the sliding rod 46 to move in the direction away from the second fixed frame plate 47. Under the traction movement of the first slider 13 on the first L-shaped connecting rod 49, the third slider 54 on the second L-shaped connecting rod 53 and the fourth slider 58 on the third L-shaped connecting rod 56, the first limiting ring 51 on the first L-shaped connecting rod 49, the second limiting ring 55 on the second L-shaped connecting rod 53 and the third limiting ring 57 on the third L-shaped connecting rod 56 are moved away from each other, which facilitates the placement operation of the culture container 7 clamped by the clamping plate 18. Step 6: When the extension plate 19 retracts after the culture container 7 is placed, the sliding tooth plate 41 moves toward the outside of the second support seat 61 under the reset action of the reset spring 42. Then, under the connection relationship of the rotating gear 43, the L-shaped tooth plate 45 drives the driving plate 48 on the sliding rod 46 to move toward the second fixed frame plate 47. Under the traction and movement of the first slider 13 on the first L-shaped link 49, the third slider 54 on the second L-shaped link 53 and the fourth slider 58 on the third L-shaped link 56, the first limiting ring 51 on the first L-shaped link 49, the second limiting ring 55 on the second L-shaped link 53 and the third limiting ring 57 on the third L-shaped link 56 are brought close to each other, which facilitates the centering and positioning of the placed culture container 7, thereby facilitating the observation operation of the microscope 60 above.

[0049] It should be noted that the components in this application are all universal standard parts or components well known to those skilled in the art, which effectively solve the technical problems raised in the background technology.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A culture container transfer device for cell culture, comprising a culture box (1) and a second support seat (61), characterized in that: The incubator (1) is provided with an electric door (2) for placing a culture container (7) on the outside, and a microscope (60) for observation is installed on the second support seat (61) via a vertical plate (59), and further comprises: A position limiting transmission mechanism is installed inside the incubator (1) and is used to transport the culture containers (7) one by one. The position limiting transmission mechanism includes a T-shaped position limiting plate (9) for limiting the position of the culture containers (7) one by one. The T-shaped position limiting plate (9) is provided with a first position limiting wheel (10) and a second position limiting wheel (11) for guiding and limiting the movement of the culture containers (7); A material taking and placing mechanism is installed on the outside of the incubator (1) and is used to take out the culture container (7) for placement and observation. The material taking and placing mechanism includes a second sliding door (27) and a first sliding door (26) for sealing the incubator (1) and connecting the material taking. The first sliding door (26) is connected to one side of the second sliding door (27) in a limited sliding manner. A clamping plate (18) for taking the material is provided on one side of the first sliding door (26). One side of the first sliding door (26) and the second sliding door (27) are connected to a first fixed frame plate (31). The first fixed frame plate (31) is fixedly installed inside the incubator (1). The first fixed frame plate (31) is provided with a second guide groove (32) for driving the clamping plate (18) to turn the material taking. A positioning observation mechanism is mounted on the second support seat (61) and is driven to open and center by the material placement mechanism for positioning and centering the placed culture container (7). The positioning observation mechanism includes a first L-shaped connecting rod (49), a second L-shaped connecting rod (53) and a third L-shaped connecting rod (56) for centering drive. The first L-shaped connecting rod (49), the second L-shaped connecting rod (53) and the third L-shaped connecting rod (56) are all movably mounted on the second support seat (61) through a driving plate (48).

2. The culture container transfer device for cell culture according to claim 1, characterized in that: The position-limiting transport mechanism further comprises a first transport platform (4), a second transport platform (5) and a third transport platform (6) for transporting the culture container (7); the first transport platform (4), the second transport platform (5) and the third transport platform (6) are docked and connected one by one; the third transport platform (6) is provided with a position-limiting rod (8) for limiting the position of a single transported culture container (7); the first transport platform (4), the second transport platform (5) and the third transport platform (6) are all installed inside the incubator (1) through the first support seat (3).

3. The culture container transfer device for cell culture according to claim 2, characterized in that: The position limiting transmission mechanism also includes a first electric push rod (16) for driving a T-shaped position limiting plate (9) for position limiting transmission, wherein the T-shaped position limiting plate (9) is rotatably mounted on one side of the third conveying platform (6) via a first connecting shaft (12), and one end of the T-shaped position limiting plate (9) is rotatably connected to a docking plate (15) via a second connecting shaft (14), and the docking plate (15) is fixedly connected to a piston rod of the first electric push rod (16), and the first electric push rod (16) is rotatably mounted on the first conveying platform (4) via a third connecting shaft (17).

4. The culture container transfer device for cell culture according to claim 1, characterized in that: The material taking and placing mechanism also includes a second electric push rod (23) and a fixed frame (24) for clamping the culture container (7) for material taking and placing connection drive, the clamping plate (18) is rotatably connected to the extension plate (19) through the fourth connecting shaft (20), the extension plate (19) is fixedly connected to the piston rod of the second electric push rod (23), the second electric push rod (23) is fixedly installed on the sealing plate (25) through the fixed frame (24), the sealing plate (25) is fixedly installed on one side of the first sliding door (26), one end of the clamping plate (18) is rotatably connected to the docking link (21), the other end of the docking link (21) is hingedly installed on the hinge seat (22), the hinge seat (22) is fixedly installed on the fixed frame (24), and a push plate (39) is also provided on the extension plate (19).

5. The culture container transfer device for cell culture according to claim 4, characterized in that: The material picking and placing mechanism also includes a multi-stage electric telescopic rod (33) and a motor (36) for driving the clamping plate (18) to move and turn. The first sliding door (26) is connected to the second guide groove (32) on the rotating plate (28) and the first fixed frame plate (31) through a second slider (30) in a limited sliding manner. The second slider (30) is movably connected to the piston rod of the multi-stage electric telescopic rod (33). The multi-stage electric telescopic rod (33) is rotatably installed on the first fixed frame plate (31) through a rotating block (34). The rotating plate (28) is provided with a first guide groove (29) for adapting the sliding connection with the second slider (30). The rotating plate (28) is fixedly connected to the output shaft of the motor (36). The motor (36) is fixedly installed inside the incubator (1) through a fixed block (35). The second guide groove (32) is provided with a first area (37) and a second area (38). The second sliding door (27) is fixedly installed on the rotating plate (28).

6. The culture container transfer device for cell culture according to claim 1, characterized in that: The positioning and observation mechanism also includes a first limiting ring (51), a second limiting ring (55) and a third limiting ring (57) for positioning the culture container (7) for centering processing, wherein the first limiting ring (51) is fixedly connected to one end of the first L-shaped connecting rod (49), the second limiting ring (55) is fixedly connected to one end of the second L-shaped connecting rod (53), and the third limiting ring (57) is fixedly connected to one end of the third L-shaped connecting rod (56). The first L-shaped connecting rod (49) is rotatably connected to the second support seat (61) through the sixth connecting shaft (50), and the second L-shaped connecting rod (53) and the third L-shaped connecting rod (56) are both rotatably connected to the second support seat (61) through the seventh connecting shaft (52).

7. The culture container transfer device for cell culture according to claim 6, characterized in that: The positioning observation mechanism also includes a driving plate (48) and a sliding rod (46) for driving the first limiting ring (51), the second limiting ring (55) and the third limiting ring (57) to be connected to each other, wherein the driving plate (48) is connected to the second fixed frame plate (47) by limiting sliding through the sliding rod (46), and the second fixed frame plate (47) is fixedly mounted on the second support seat (61), the first L-shaped connecting rod (49) is connected to the driving plate (48) by limiting sliding through the first slider (13), the second L-shaped connecting rod (53) is connected to the driving plate (48) by limiting sliding through the third slider (54), and the third L-shaped connecting rod (56) is connected to the driving plate (48) by limiting sliding through the fourth slider (58).

8. The culture container transfer device for cell culture according to claim 7, characterized in that: The positioning observation mechanism also includes an L-shaped tooth plate (45) and a sliding tooth plate (41) for driving the driving plate (48) to perform a movement control connection, wherein the L-shaped tooth plate (45) is fixedly connected to one end of the sliding rod (46), and the L-shaped tooth plate (45) and the sliding tooth plate (41) are both limitedly slidably connected to the inside of the second support seat (61), and the L-shaped tooth plate (45) and the sliding tooth plate (41) are both meshedly connected with a rotating gear (43), and the rotating gear (43) is rotatably connected to the inside of the second support seat (61) through a fifth connecting shaft (44), and a return spring (42) is provided at the connection between one end of the sliding tooth plate (41) and the inside of the second support seat (61), and a pressing plate (40) is provided at the other end of the sliding tooth plate (41), and a push plate (39) is adapted to be connected to one side of the pressing plate (40).

9. A method for using a culture container transfer device for cell culture, applied to the culture container transfer device for cell culture according to any one of claims 1 to 8, characterized in that: The method for using the culture container transfer device for cell culture comprises the following steps: Step 1: Open the electric door (2) on the incubator (1), place the culture container (7) to be cultured for cells on the first conveyor platform (4) for transportation, and after the first conveyor platform (4), the second conveyor platform (5) and the third conveyor platform (6) are connected one by one, multiple culture containers (7) are stacked on one side of the T-shaped limiting plate (9) on the third conveyor platform (6), and the first limiting wheel (10) on the T-shaped limiting plate (9) limits the culture container (7); Step 2: When the culture container (7) needs to be transported out individually for material collection, the first electric push rod (16) drives the T-shaped limit plate (9) to rotate under the connection relationship of the first connecting shaft (12). At this time, the first electric push rod (16) is in a retracted state, so the first limit wheel (10) on the T-shaped limit plate (9) is away from the limit of the culture container (7). The second limit wheel (11) on the T-shaped limit plate (9) can push the culture container (7) at this location to the side close to the limit rod (8), and then the first electric push rod (16) is reset and extended. At this time, the T-shaped limit plate (9) is reset to its original position to perform the limit processing of the next culture container (7); Step 3: The multi-stage electric telescopic rod (33) drives the first sliding door (26) and the splint (18) connected to the second slider (30) to retract and move, and then retract under the guidance of the second guide groove (32) on the first fixed frame plate (31). When the multi-stage electric telescopic rod (33) reaches the curved position close to the second guide groove (32), the multi-stage electric telescopic rod (33) no longer retracts and moves, and the motor (36) drives the rotating plate (28) and the splint (18) on the first sliding door (26) to rotate 90 degrees, that is, at this time, the second slider (30) moves from the first area (37) to the second area (38); Step 4: When the second slider (30) moves to the second area (38), the clamping plate (18) moves to the clamping range of the culture container (7) on the third conveyor (6), and the second electric push rod (23) drives the extension plate (19) to retract and move. At this time, under the connection relationship of the docking connecting rod (21), the clamping plates (18) on the extension plate (19) are close to each other to complete the clamping and fixing operation of the culture container (7). Then the motor (36) resets and rotates in the opposite direction, the multi-stage electric telescopic rod (33) extends and moves, and the clamping plate (18) moves the clamped culture container (7) to the second support seat (61) for placement; Step 5: When the multi-stage electric telescopic rod (33) pushes the second slider (30) to move forward under the guidance of the second guide groove (32), the push plate (39) on the extension plate (19) can push the sliding tooth plate (41) on the pressing plate (40) to move closer to the inside of the second support seat (61). Under the connection relationship of the rotating gear (43), the L-shaped tooth plate (45) drives the driving plate (48) on the sliding rod (46) to move in the direction away from the second fixed frame plate (47). Under the pulling and moving action of the first slider (13) on the first L-shaped connecting rod (49), the third slider (54) on the second L-shaped connecting rod (53), and the fourth slider (58) on the third L-shaped connecting rod (56), the first limiting ring (51) on the first L-shaped connecting rod (49), the second limiting ring (55) on the second L-shaped connecting rod (53), and the third limiting ring (57) on the third L-shaped connecting rod (56) are moved away from each other, thereby facilitating the placement operation of the culture container (7) clamped by the clamping plate (18); Step 6: When the culture container (7) is placed and the extension plate (19) is retracted, the sliding tooth plate (41) moves toward the outside of the second support seat (61) under the reset action of the reset spring (42). Then, under the connection relationship of the rotating gear (43), the L-shaped tooth plate (45) drives the driving plate (48) on the sliding rod (46) to move toward the second fixed frame plate (47). The first slider (13) and the second L-shaped connecting rod (49) are connected. Under the pulling and moving action of the third slider (54) on the L-shaped connecting rod (53) and the fourth slider (58) on the third L-shaped connecting rod (56), the first limiting ring (51) on the first L-shaped connecting rod (49), the second limiting ring (55) on the second L-shaped connecting rod (53) and the third limiting ring (57) on the third L-shaped connecting rod (56) are brought close to each other, which facilitates the centering and positioning of the placed culture container (7), thereby facilitating the observation operation of the microscope (60) above.

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