High-durability concrete for inducing adhesion of marine fixing organisms and preparation method of high-durability concrete

Abstract

The invention provides high-durability concrete for inducing adhesion of marine fixing organisms and a preparation method, and belongs to the crossing field of marine fixing organisms and marine concretes.The concrete is prepared by adopting a porous carrier loaded with induction bacteria, and the impregnation process of the porous carrier loaded with the induction bacteria is optimized; according to the present invention, the barnacle can be induced to adsorb to the concrete surface, the chloride ion resistance of the concrete can be improved, and the concrete has characteristics of simple component, low preparation cost, green environmental protection, and wide application prospect, and can provide more effective protection for the loaded bacteria, and can induce the barnacle to adsorb to the concrete surface. The goal of high durability of the concrete for underwater ocean engineering can be achieved.

Description

Technical Field

[0001] The present invention belongs to the intersection field of marine sessile organisms and marine concrete, and particularly relates to a high-durability concrete capable of inducing the attachment of marine sessile organisms and a preparation method thereof. Background Art

[0002] To minimize the damage caused by marine corrosion, countries around the world attach great importance to protecting concrete in marine environments. Traditional protection methods include the use of specialized materials, surface coatings, and anti-corrosion agents, but these methods are associated with high costs, difficult maintenance, and environmental pollution. Therefore, there is an urgent need to find more economical, environmentally friendly, and low-cost protection methods. In recent years, numerous experts and researchers have conducted research on the microbial repair of concrete cracks. Results indicate that microorganisms deposit calcium minerals on the concrete surface, forming an effective protective layer that can effectively repair surface damage and improve its impermeability. Marine environments are home to a large number of marine biofilms, such as barnacles, oysters, and mussels, which attach and grow on the surfaces of marine concrete structures. The interaction between marine biofilms and material surfaces generally occurs in two main stages: microfouling and macrofouling. Initially, the material surface is colonized by marine bacteria, forming a bacterial biofilm, also known as microfouling. This bacterial biofilm facilitates the colonization of other microorganisms, such as cyanobacteria, fungi, diatoms, barnacles, algae, and protozoa. Marine biofilms secrete protein colloids on the surface, forming a protective biofilm layer that provides some protection against concrete erosion and damage. Compared with traditional protection methods, marine biofilm has the advantages of low carbon, environmental protection, and cost saving. At the same time, marine biofilm also has the characteristics of self-repair and self-renewal.

[0003] Existing technologies, such as the Chinese invention patent with authorization announcement number CN 111268959 B, disclose a concrete and preparation method for inducing the attachment and promoting the growth of marine sessile organisms, which belongs to the intersection of marine sessile organisms and marine concrete. The material components of the present invention include: cementitious materials, crushed stone, sand, water, dark pigment, calcium carbonate powder, trace elements and superplasticizer. The present invention uses zinc sulfate, potassium sulfate, potassium nitrate, iron sulfate, zinc phosphate, ammonium nitrate, potassium phosphate, ammonium phosphate, iron phosphate and calcium phosphate as trace elements to be incorporated into concrete, and by modifying these substances, the strength and impermeability of the concrete remain basically unchanged, and the induced attachment rate of oyster larvae is greatly increased. This concrete has complex components, high cost, limited concrete durability, and is prone to causing problems such as marine environmental pollution. Summary of the Invention

[0004] The purpose of the present invention is to provide a high-durability concrete for underwater marine engineering and a preparation method thereof. The concrete has simple composition, low preparation cost, is green and environmentally friendly, has high durability, and has broad application prospects in the field of marine concrete engineering materials.

[0005] The technical solutions adopted by the present invention to achieve the above-mentioned purpose are: The invention discloses a high-durability concrete for inducing the attachment of marine sessile organisms, comprising the following components in parts by weight: 100-120 parts by weight of a cementitious material, 140-220 parts by weight of a coarse aggregate, 120-160 parts by weight of a fine aggregate, 30-80 parts by weight of a porous carrier loaded with inducing bacteria, 0.8-1 part by weight of a water reducer, 35-45 parts by weight of water, and 2-6 parts by weight of a substrate; The substrates include urea and calcium lactate, and the inducing bacteria include Bacillus pasteurianus. Using a porous carrier loaded with Bacillus pasteurianus to repair concrete cracks can achieve excellent repair results. Furthermore, concrete prepared with this porous carrier not only exhibits excellent self-repairing capabilities but also induces marine anchors, particularly barnacles, to rapidly adsorb onto the concrete surface, forming a dense layer of barnacle bioglue that resists chloride ion corrosion. Combined with microbial self-repair technology, this coupled effect further enhances concrete's chloride resistance.

[0006] Preferably, the preparation method of the porous carrier loaded with induced bacteria comprises: preparing the induced bacteria into a spore suspension, adding nutrients, konjac glucomannan, salicyloyl phytosphingosine, and diethylene glycol ethyl ether, adding the porous carrier, mixing evenly, vacuum impregnating for 50 min-120 min, and drying at 40-45 ° C to constant weight to complete the loading.

[0007] Preferably, the mass-to-volume ratio of the salicyloyl phytosphingosine to diethylene glycol ethyl ether is 2-7g:50-80mL. Salicyloyl phytosphingosine has both hydrophilic and hydrophobic groups. In the presence of a certain proportion of diethylene glycol ethyl ether in the impregnation solution, the hydrophobic groups can interact with the volcanic rock, while the hydrophilic groups interact with the bacteria, providing more loading sites for the volcanic rock to load the bacteria, increasing the loading rate of the induced bacteria and thereby enhancing the induction effect on marine anchors, especially barnacles. The coupled effect of the two further improves the chloride ion resistance of the concrete and improves its durability.

[0008] Preferably, the inducing bacteria are a combination of Bacillus pasteurianus, Bacillus cladodes, and Sporosarcina licheniformis.

[0009] Preferably, the inducing bacteria are a combination of Bacillus pasteurianus and Bacillus cladocerus.

[0010] Preferably, the inducing bacteria are a combination of Bacillus pasteurianus and Sporosarcina licheniformis.

[0011] Preferably, the above-mentioned inducing bacteria is Bacillus pasteurianus.

[0012] Preferably, the above-mentioned cementitious material is one or both of sulphoaluminate cement and silicate cement added with mineral admixtures.

[0013] Preferably, the above-mentioned cementitious material is silicate cement added with mineral admixtures.

[0014] More preferably, the mineral admixtures in the silicate cement with mineral admixtures include one or more of fly ash, mineral powder, and silica fume.

[0015] More preferably, the components of the silicate cement with mineral admixtures are: cement, fly ash, mineral powder, and silica fume, with the weight ratio being: (45-70): (20-30): (25-35): (1-3). Furthermore, the components of the silicate cement with mineral admixtures are: cement, fly ash, mineral powder, and silica fume, with the weight ratio being: (45-50): (22-26): (25-30): (1-2).

[0016] Preferably, the coarse aggregate is one or more of granite crushed stone, limestone crushed stone and basalt crushed stone.

[0017] Preferably, the fine aggregate is one or both of river sand and desalinated sea sand.

[0018] Preferably, the water reducer is a polycarboxylate water reducer.

[0019] Preferably, the mass ratio of the urea to calcium lactate is 1:1-1.2.

[0020] Preferably, the porous carrier is one or more of ceramsite, volcanic rock, activated carbon, and diatomaceous earth.

[0021] Preferably, the concentration of the spore suspension is 4×10 9 -8×10 10 cells / mL.

[0022] Preferably, the mass volume ratio of the konjac glucomannan to the spore suspension is 4-10 g:1 L.

[0023] Preferably, the mass volume ratio of the salicyloyl phytosphingosine to the spore suspension is 2-7 g:1 L.

[0024] Preferably, the mass-to-volume ratio of the porous carrier to the spore suspension is 7-12 g:1 mL.

[0025] Preferably, the nutrient is yeast extract. More preferably, the mass-to-volume ratio of the yeast extract to the spore suspension is 20-25 g:1 L.

[0026] Preferably, in the preparation method of the porous carrier loaded with inducing bacteria, after vacuum impregnation and before drying the carrier, glycerophosphoinositol choline salt is added, stirred evenly and allowed to stand for 15-25 minutes. Glycerophosphoinositol choline salt contains strong polar groups such as phosphate, choline and inositol, which can promote the porous carrier loaded with inducing bacteria to form small-particle microaggregates, provide more effective protection for the loaded bacteria, reduce direct contact between bacteria and cement matrix, increase bacterial activity and survival rate, and thus enhance the inducing effect on marine anchors, especially barnacles. Under the coupling effect of the two, the chloride ion resistance of concrete is further improved, thereby achieving the high durability goal of concrete for underwater marine engineering.

[0027] Preferably, the mass volume ratio of the above-mentioned glycerophosphoinositol choline salt to the spore suspension is 3-8 g:1 L.

[0028] The present invention discloses a method for preparing high-durability concrete capable of inducing attachment of marine sessile organisms, comprising: S1: Weigh 100-120 parts by weight of cementitious material, 140-220 parts by weight of coarse aggregate, 120-160 parts by weight of fine aggregate, 30-80 parts by weight of porous carrier loaded with induced bacteria, 0.8-1 part by weight of water reducer, 35-45 parts by weight of water, and 2-6 parts by weight of substrate; S2: First, pre-mix the coarse aggregate and fine aggregate in a mixer for 30-60 seconds, then add the cementitious material and stir for 60-90 seconds, then add the porous carrier loaded with induced bacteria and stir for 90-120 seconds, and finally add the pre-mixed mixture of substrate, water reducer and water, stir for 90-120 seconds, and then pour, vibrate, cure and finish.

[0029] Preferably, the substrate comprises urea and calcium lactate, and the inducing bacteria is a combination of one or more of Bacillus pasteurianus, Bacillus cladocerus, and Sporosarcina licheniformis.

[0030] The purpose of the present invention is to provide a high-durability concrete for underwater marine engineering and a preparation method thereof. The concrete has simple composition, low preparation cost, is green and environmentally friendly, has high durability, and has broad application prospects in the field of marine concrete engineering materials.

[0031] Since the present invention uses a porous carrier loaded with induced bacteria to prepare concrete, it has the following beneficial effects: the concrete is self-repairable and can induce marine anchors, especially barnacles, to adsorb to the concrete surface, forming a dense layer of barnacle bioglue on the concrete surface to resist chloride ion corrosion. Combined with the microbial self-repair technology, the coupling effect of the two further improves the concrete's resistance to chloride ions and enhances the durability of the concrete.

[0032] The present invention also optimizes the impregnation process of the porous carrier for loading induced bacteria, thereby having the following beneficial effects: it can increase the loading rate of the porous carrier and provide more effective protection for the loaded bacteria, reduce direct contact between the bacteria and the cement matrix, improve the activity and survival rate of the bacteria, and the self-repair ability of the concrete produced is stronger, and it can quickly induce barnacles to adsorb to the concrete surface, thereby achieving the goal of high durability of concrete for underwater marine engineering.

[0033] Therefore, the present invention provides a high-durability concrete and a preparation method thereof, which has simple ingredients, low preparation cost, is green and environmentally friendly, has high durability, and has broad application prospects in the field of marine concrete engineering materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 This is the measurement result of urea decomposition concentration in Test Example 1.

[0035] Figure 2 OD of the bacterial solution in Experiment 1 600 The measurement results of the value.

[0036] Figure 3 This is the SEM characterization image of the crack filling material of the specimen in Experimental Example 2.

[0037] Figure 4 The results of the barnacle count determination in Test Example 3 are shown.

[0038] Figure 5 The results of the electric flux measurement in Experiment 3 are shown.

[0039] Figure 6 This is the measurement result of the chloride ion diffusion coefficient in Test Example 3. DETAILED DESCRIPTION

[0040] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0041] The following first describes the concepts involved in this application with reference to the accompanying drawings. It should be noted that the following description of each concept is intended only to make the content of this application easier to understand and does not limit the scope of protection of this application. At the same time, the embodiments and features in the embodiments of this application can be combined with each other unless there is a conflict. The following detailed description of this application will be made with reference to the accompanying drawings and in conjunction with the embodiments.

[0042] In the embodiments of the present invention, unless otherwise stated, the equipment and materials used in the present invention are purchased from the market.

[0043] Example 1: 1. A method for preparing a porous carrier loaded with inducing bacteria, comprising: 1.1 The inducing bacteria was Bacillus pasteurianus, purchased from Shanghai Microorganism Collection Center (SHBCC), with the number ATCC-11859.

[0044] 1.2 The culture medium contains 5 g peptone, 3 g yeast extract, 20 g urea, and 1 L deionized water.

[0045] 1.3 Inoculate the above culture medium with Bacillus pasteurianus at a volume ratio of 2% and incubate at 30℃ for 36 hours before use.

[0046] 1.4 Centrifuge the bacterial solution, wash the bacteria with deionized water and centrifuge again. Repeat this process several times to obtain concentrated bacterial spores. Then dilute with sterile deionized water to obtain 7×10 9 cells / mL of spore suspension.

[0047] 1.5 Take 100 mL of spore suspension, add 2.3 g of yeast extract and 800 g of volcanic rock (volcanic rock particle size is 1-2 mm, dried at 105°C before use), mix well, and adsorb and impregnate at -0.08 MPa for 1 h. Remove the volcanic rock and dry it at 40°C to constant weight to obtain volcanic rock loaded with Bacillus pasteurianus.

[0048] 2. A method for preparing high-durability concrete, comprising: 2.1 The cementitious material is 48wt% ordinary Portland cement, blended with 24wt% fly ash, 27wt% mineral powder (granulated blast furnace slag powder), and 1wt% silica fume. The coarse aggregate is granite crushed stone with a particle size of 5-20mm and good gradation. The fine aggregate is river sand with a particle size of 1-4mm and good gradation. The water meets the standard for water used in concrete (JGJ63-2006).

[0049] 2.2 Weigh 100 parts by weight of cementitious material, 220 parts by weight of coarse aggregate, 160 parts by weight of fine aggregate, 50 parts by weight of porous carrier loaded with induced bacteria, 0.8 parts by weight of polycarboxylate water reducer, 40 parts by weight of water, 2 parts by weight of urea and 2 parts by weight of calcium lactate.

[0050] 2.3 Pre-mix the coarse aggregate and fine aggregate in a mixer for 45 seconds, then add the cementitious material and stir for 90 seconds. Then add the volcanic rock loaded with Bacillus pasteurianus prepared in 1 above and stir for 120 seconds. Finally, add the pre-mixed mixture of urea and calcium lactate, polycarboxylate water reducer and water. After stirring for 120 seconds, place the mixture in a mold pre-coated with oil release agent and vibrate on a vibration table until slurry comes out of the surface of the specimen. During the vibration process, use a shovel to tap along the mold wall to ensure comprehensive vibration and prevent segregation and water bleeding caused by excessive vibration.

[0051] 2.4 The specimens were covered with plastic film and allowed to stand at 22°C for 24 h. The molds were then removed and numbered. The finished specimens were obtained after curing in a standard concrete curing room for 28 days.

[0052] Example 2: 1. A method for preparing a porous carrier loaded with inducing bacteria, comprising: 1.1 The inducing bacteria was Bacillus pasteurianus, purchased from Shanghai Microorganism Collection Center (SHBCC), with the number ATCC-11859.

[0053] 1.2 The culture medium contains 5 g peptone, 3 g yeast extract, 20 g urea, and 1 L deionized water.

[0054] 1.3 Inoculate the above culture medium with Bacillus pasteurianus at a volume ratio of 2% and incubate at 30℃ for 36 hours before use.

[0055] 1.4 Centrifuge the bacterial solution, wash the bacteria with deionized water and centrifuge again. Repeat this process several times to obtain concentrated bacterial spores. Then dilute with sterile deionized water to obtain 7×10 9 cells / mL of spore suspension.

[0056] 1.5 Take 100 mL of spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), and 800 g of volcanic rock (volcanic rock particle size 1-2 mm, dried at 105°C before use), mix well, and adsorb and impregnate at -0.08 MPa for 1 h. Remove the volcanic rock and dry it at 40°C to constant weight to obtain volcanic rock loaded with Bacillus pasteurianus.

[0057] 2. A method for preparing high-durability concrete, comprising: 2.1 The cementitious material is 48wt% ordinary Portland cement, blended with 24wt% fly ash, 27wt% mineral powder (granulated blast furnace slag powder), and 1wt% silica fume. The coarse aggregate is granite crushed stone with a particle size of 5-20mm and good gradation. The fine aggregate is river sand with a particle size of 1-4mm and good gradation. The water meets the standard for water used in concrete (JGJ63-2006).

[0058] 2.2 Weigh 100 parts by weight of cementitious material, 220 parts by weight of coarse aggregate, 160 parts by weight of fine aggregate, 50 parts by weight of porous carrier loaded with induced bacteria, 0.8 parts by weight of polycarboxylate water reducer, 40 parts by weight of water, 2 parts by weight of urea and 2 parts by weight of calcium lactate.

[0059] 2.3 Pre-mix the coarse aggregate and fine aggregate in a mixer for 45 seconds, then add the cementitious material and stir for 90 seconds. Then add the volcanic rock loaded with Bacillus pasteurianus prepared in 1 above and stir for 120 seconds. Finally, add the pre-mixed mixture of urea and calcium lactate, polycarboxylate water reducer and water. After stirring for 120 seconds, place the mixture in a mold pre-coated with oil release agent and vibrate on a vibration table until slurry comes out of the surface of the specimen. During the vibration process, use a shovel to tap along the mold wall to ensure comprehensive vibration and prevent segregation and water bleeding caused by excessive vibration.

[0060] 2.4 The specimens were covered with plastic film and allowed to stand at 22°C for 24 h. The molds were then removed and numbered. The finished specimens were obtained after curing in a standard concrete curing room for 28 days.

[0061] Example 3: The difference from Example 1 is that: 1.5 Take 100 mL of the spore suspension, add 2.3 g of yeast extract, 0.4 g of salicyloyl phytosphingosine, 7 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size is 1-2 mm, dried at 105°C before use), mix well, adsorb and immerse at -0.08 MPa for 1 hour, remove the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0062] Example 4: The difference from Example 2 is that: 1.5 Take 100 mL of the spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 0.4 g of salicyloyl phytosphingosine, 7 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and immerse at -0.08 MPa for 1 hour, remove the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0063] Example 5: The difference from Example 4 is that: 1.5 Take 100 mL of the spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 0.4 g of salicyloyl phytosphingosine, 3 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and immerse at -0.08 MPa for 1 hour, remove the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0064] Example 6: The difference from Example 4 is that: 1.5 Take 100 mL of the spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 0.4 g of salicyloyl phytosphingosine, 15 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and immerse at -0.08 MPa for 1 hour, remove the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0065] Example 7: The difference from Example 4 is that: 1.5 To 100 mL of the spore suspension, 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 0.4 g of salicyloyl phytosphingosine, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use) were added, mixed evenly, and adsorbed and impregnated at -0.08 MPa for 1 h. The volcanic stone was removed and dried at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0066] Example 8: The difference from Example 4 is that: 1.5 Take 100 mL of the spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 7 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and immerse at -0.08 MPa for 1 hour, remove the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0067] Example 9: The difference from Example 2 is that: 1.5 Take 100 mL of spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and soak at -0.08 MPa for 1 hour, add 0.6 g of pullulan (effective substance content 99%), mix well, let stand for 15 minutes, take out the volcanic stone, and dry it at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0068] Example 10: The difference from Example 2 is that: 1.5 Take 100 mL of spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and impregnate at -0.08 MPa for 1 hour, add 0.5 g of glycerophosphoinositol choline salt, mix well, let stand for 15 minutes, add and remove volcanic stone, and dry at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0069] Example 11: The difference from Example 4 is that: 1.5 Take 100 mL of spore suspension, add 2.3 g of yeast extract, 0.8 g of konjac glucomannan (effective substance content 98.8%), 0.4 g of salicyloyl phytosphingosine, 7 mL of diethylene glycol ethyl ether, and 800 g of volcanic stone (volcanic stone particle size 1-2 mm, dried at 105°C before use), mix well, adsorb and soak at -0.08 MPa for 1 hour, add 0.5 g of glycerophosphoinositol choline salt, mix well, let stand for 15 minutes, add and remove volcanic stone, and dry at 40°C to constant weight to obtain volcanic stone loaded with Bacillus pasteurianus.

[0070] Test Example 1: 1. Determination of loading activity: The volcanic rock loaded with Bacillus pasteurianus was added to 100 mL of test solution (1 g / L yeast extract and 20 g / L urea), sealed and placed at 30 ° C. After 48 h, the ammonium ion (NH 4+ ) concentration, and the urea decomposition concentration of bacteria was converted. The results of urea decomposition concentration are shown in Figure 1 .

[0071] Depend on Figure 1It can be seen that the urea decomposition concentration of the volcanic rock loaded with Bacillus pasteurianus prepared in Example 3 is greater than that in Example 1, and the urea decomposition concentration of Example 4 is greater than that in Example 2, Example 5, Example 6, Example 7, and Example 8. The difference between Examples 2, 5, 6, 7, and 8 is not much, indicating that the addition of salicyloyl phytosphingosine and diethylene glycol ethyl ether in a mass volume ratio of 2-7 g: 50-80 mL during the impregnation loading process can provide more loading sites for the porous carrier and improve the loading rate of the induced bacteria.

[0072] 2. Determination of the protective effect of porous carriers on microorganisms: Simulated cement-based material pore solution: 0.002 mol / L Ca(OH)2, 0.25 mol / L NaOH, 0.54 mol / L KOH, 0.003 mol / L K2SO4, and the pH was adjusted to 13.54.

[0073] The volcanic rock loaded with Bacillus pasteurianus was immersed in the pore solution of the simulated cement-based material. After immersion for 56 days, it was taken out, dried and crushed. 30g of the rock was placed in 300mL of microbial culture medium and cultured with shaking at 30℃ and 150rpm for 120h. The OD value of the obtained bacterial solution was tested. 600 Value. Bacterial solution OD 600 The results of the determination of the value can be seen in Figure 2 .

[0074] Depend on Figure 2 It can be seen that the OD value of the bacterial solution measured by the volcanic rock loaded with Bacillus pasteurianus prepared in Example 10 is 600 The values ​​are greater than those of Example 2, and those of Example 11 are greater than those of Example 4, indicating that the addition of glycerophosphoinositol choline salt during the impregnation process can improve the effective protection of the porous carrier for loaded bacteria.

[0075] Test Example 2: The 100mm×50mm finished concrete specimens that had been cured to the age of 7 days were taken out and cracks with a width of about 0.4mm were created. First, a stainless steel hose clamp was used to cover the specimen, and the hose clamp was tightened with a screwdriver to ensure that uniform cracks were created. Then, the clamped specimen was placed upright on the pressure testing machine, and pressure was applied until obvious cracks appeared on the specimen. The loading was immediately stopped, the specimen was removed and the hose clamp was adjusted to create a crack with a width of about 0.4 mm. The hose clamp was then tightened again to prevent the crack width of the specimen from changing due to loosening during the repair process. In order to ensure that there was sufficient humidity at the crack, the concrete beams with prefabricated cracks on the bottom were inverted and immersed in water according to different groups to prevent mutual influence, and were cured and repaired in a curing box at 30±2℃ for 28 days. After 28 days of repair in water, the crack fillers of the specimens were characterized by SEM. The SEM characterization of the crack fillers of the specimens is shown in Figure 3 .

[0076] Depend on Figure 3 It can be seen that compared with Example 2, the crack filler particles of Example 4 are uniform in size, the particles are well bonded, and more cracks and pores are filled, indicating that the concrete specimen prepared in Example 4 has a higher concentration of Bacillus pasteurianus and a better self-healing effect.

[0077] Test Example 3: The test pieces prepared in the above Examples 1-11 were subjected to exposure tests.

[0078] 1. Exposure experiment plan: 1.1. The actual marine exposure site is located at the Wulong Passenger Terminal in Shengsi County, Zhoushan City, Zhejiang Province. It is located in the easternmost part of Zhejiang Province and the northernmost part of the Zhoushan Archipelago. It is located in the East China Sea at the confluence of the Yangtze River and Qiantang River estuaries. Its precise location is 30°42′5.1876″N, 122°31′13.9296″E, approximately 79 km and 114 km from Shanghai and Ningbo, respectively.

[0079] 1.2. Finished specimens measuring 150 mm × 150 mm × 150 mm were placed in the tidal range area of ​​Wulong Wharf on Shengsi Island, Zhoushan City, Zhejiang Province (April 2024–October 2024). To prevent loss of the concrete specimens due to strong winds and waves during the test, custom steel cages were used to secure the concrete specimens. The concrete specimens were placed in three locations on the same plane.

[0080] 1.3. After 180 days of exposure, retrieve the test specimens. Then, perform the cube compression test, electric flux test, chloride ion diffusion coefficient test, rapid carbonization test, and microscopic test according to the test procedures. Each test should be repeated three times, and the arithmetic average should be calculated.

[0081] 2. Barnacle attachment number test The specimens were retrieved after 180 days of exposure and the average number of barnacles attached to the specimens was calculated. The results of the determination of the number of barnacles are shown in Figure 4 .

[0082] Depend on Figure 4It can be seen that the number of barnacles in Example 3 is greater than that in Example 1, and the number of barnacles in Example 4 is greater than that in Examples 2, 5, 6, 7, and 8, and the differences among Examples 2, 5, 6, 7, and 8 are not much different, indicating that the addition of salicyl phytosphingosine and diethylene glycol ethyl ether at a mass-to-volume ratio of 2-7 g:50-80 mL during the impregnation loading process can increase the loading rate of the porous carrier for the induced bacteria, thereby inducing more marine anchor barnacles to adsorb to the concrete surface. The number of barnacles in Example 10 is greater than that in Example 2, and in Example 11 is greater than that in Example 4, indicating that the addition of glycerophosphoinositol choline salt during the impregnation process provides more effective protection for the loaded bacteria, improves the activity and survival rate of the bacteria, and thus induces more marine anchor barnacles to adsorb to the concrete surface.

[0083] 3. Durability test 3.1 Chloride ion flux test The test steps are carried out in accordance with the Standard for Test Methods for Long-term Performance and Durability of Ordinary Concrete (GB / T50082-2009), as follows: 3.1.1 Coring of specimens: After retrieving the specimens from the actual sea exposure site, a concrete core drill (HZ-20) shall be used to drill and coring, and the core drilling depth shall not be less than 70 mm so that it can be subsequently cut into specimens of 50 ± 2 mm.

[0084] 3.1.2 Vacuum water saturation: After the core is taken, the specimen is placed in an automatic vacuum water saturation machine for treatment. First, evacuate to vacuum conditions and maintain for 3 hours, then fill with water, soak for 1 hour, then restore to atmospheric pressure, and continue soaking for 18±2 hours.

[0085] 3.1.3 Fix the specimen; fix the specimen in the test tank and use glass glue to seal the sides of the specimen and the contact surface of the test tank to prevent leakage from the fixtures on both sides.

[0086] 3.1.4 Prepare the reagents; then, pour 0.3 mol / L sodium hydroxide solution into the positive electrode tank and 3.0% sodium chloride solution into the negative electrode tank.

[0087] 3.1.5 Collect data; Connect the positive and negative wires and turn on the power. This instrument is a fully automatic chloride ion flux meter. It does not require voltage setting. It will automatically stop and record the total flux of the specimen after the 6-hour test. The flux measurement results are shown in Figure 5 .

[0088] Depend on Figure 5It can be seen that the electric flux of Example 3 is less than that of Example 1, and the electric flux of Example 4 is less than that of Examples 2, 5, 6, 7, and 8. The differences between Examples 2, 5, 6, 7, and 8 are not significant. This indicates that the addition of salicyl phytosphingosine and diethylene glycol ethyl ether at a mass-to-volume ratio of 2-7 g:50-80 mL during the impregnation loading process can increase the loading rate of the porous carrier for the induced bacteria, induce more marine barnacles to adsorb to the concrete surface, and thus improve the density of the concrete under the coupling effect of Bacillus pasteurianus self-repair and marine barnacles. The electric flux of Example 10 is less than that of Example 2, and that of Example 11 is less than that of Example 4. This indicates that the addition of glycerophosphoinositide choline salt during the impregnation process provides more effective protection for the loaded bacteria, improves bacterial activity and survival rate, induces more marine barnacles to adsorb to the concrete surface, and thus improves the density of the concrete under the coupling effect of Bacillus pasteurianus self-repair and marine barnacles.

[0089] 3.2 The test steps are carried out in accordance with the "Standard for Test Methods of Long-term Performance and Durability of Ordinary Concrete" (GB / T50082-2009), as follows: The steps of specimen coring, vacuum saturation, specimen fixation and reagent preparation in this test are consistent with the chloride ion flux test, and will not be described in detail here. Take out the specimen and fracture the test block at a speed of 0.1kN / s under the press, and spray silver nitrate indicator on the surface. After color development, use a vernier caliper to measure the depth of the white precipitate, measure 10 times in equal parts, and take the arithmetic average. Enter the chloride ion diffusion coefficient of each channel for automatic display in the instrument. The results of the determination of the chloride ion diffusion coefficient are shown in Figure 6 .

[0090] Depend on Figure 6 It can be seen that the chloride ion diffusion coefficient of Example 3 is less than that of Example 1, and the chloride ion diffusion coefficient of Example 4 is less than that of Example 2, Example 5, Example 6, Example 7, and Example 8. The difference between Examples 2, Example 5, Example 6, Example 7, and Example 8 is not much, indicating that the addition of salicyloyl phytosphingosine and diethylene glycol ethyl ether with a mass volume ratio of 2-7g:50-80mL during the impregnation loading process can increase the loading rate of the porous carrier for the induced bacteria, induce more marine anchor barnacles to adsorb to the concrete surface, thereby enhancing the concrete's resistance to chloride ion erosion and improving the durability of the concrete. The chloride ion diffusion coefficient of Example 10 is less than that of Example 2, and that of Example 11 is less than that of Example 4, indicating that the addition of glycerophosphoinositol choline salt during the impregnation process provides more effective protection for the loaded bacteria, improves the activity and survival rate of the bacteria, induces more marine anchor barnacles to adsorb to the concrete surface, thereby enhancing the concrete's resistance to chloride ion erosion and improving the durability of the concrete.

[0091] The embodiments and / or implementation methods described above are only used to illustrate the preferred embodiments and / or implementation methods for realizing the technology of the present invention, and do not impose any form of limitation on the implementation methods of the technology of the present invention. Any person skilled in the art may make slight changes or modifications to other equivalent embodiments without departing from the scope of the technical means disclosed in the content of the present invention, but they should still be regarded as technologies or embodiments that are essentially the same as the present invention.

[0092] This article uses specific examples to illustrate the principles and implementation methods of this application. The description of the above embodiments is only used to help understand the method and core ideas of this application. The above is only the preferred implementation method of this application. It should be pointed out that due to the limitations of textual expression, there are objectively infinite specific structures. For ordinary technicians in this technical field, without departing from the principles of this application, they can also make several improvements, modifications or changes, and can also combine the above technical features in an appropriate manner; these improvements, modifications, changes or combinations, or the direct application of the inventive concept and technical solution to other occasions without improvement, should be regarded as the scope of protection of this application.

Claims

1. A highly durable concrete for inducing the attachment of marine sessile organisms, characterized in that: The invention comprises the following components in parts by weight: 100-120 parts by weight of cementitious material, 140-220 parts by weight of coarse aggregate, 120-160 parts by weight of fine aggregate, 30-80 parts by weight of porous carrier loaded with induced bacteria, 0.8-1 part by weight of water reducing agent, 35-45 parts by weight of water, and 2-6 parts by weight of substrate; The substrates include urea and calcium lactate, and the inducing bacteria include Bacillus pasteurianus.

2. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 1, characterized in that: The preparation method of the porous carrier loaded with induced bacteria includes: preparing the induced bacteria into a spore suspension, adding nutrients, konjac glucomannan, salicyloyl phytosphingosine, and diethylene glycol ethyl ether, adding the porous carrier, mixing evenly, vacuum impregnating for 50 minutes to 120 minutes, and drying at 40-45° C. to constant weight to complete the loading.

3. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 1, characterized in that: The cementitious material is one or both of sulphoaluminate cement and silicate cement added with mineral admixtures.

4. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 1, characterized in that: The mass ratio of the urea to calcium lactate is 1:1-1.

2.

5. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 1, characterized in that: The porous carrier is one or more of ceramsite, volcanic rock, activated carbon, and diatomaceous earth.

6. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 2, characterized in that: The mass volume ratio of the salicyloyl phytosphingosine and diethylene glycol ethyl ether is 2-7 g:50-80 mL.

7. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 2, characterized in that: The concentration of the spore suspension is 4×10 9 -8×10 10 cells / mL.

8. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 2, characterized in that: The mass volume ratio of the porous carrier to the spore suspension is 7-12 g:1 mL.

9. The high-durability concrete for inducing attachment of marine sessile organisms according to claim 2, characterized in that: The mass volume ratio of the konjac glucomannan to the spore suspension is 4-10 g:1 L.

10. The method for preparing a highly durable concrete capable of inducing attachment of marine sessile organisms according to claim 1, characterized in that: The following steps are involved: S1: Weigh 100-120 parts by weight of cementitious material, 140-220 parts by weight of coarse aggregate, 120-160 parts by weight of fine aggregate, 30-80 parts by weight of porous carrier loaded with induced bacteria, 0.8-1 part by weight of water reducer, 35-45 parts by weight of water, and 2-6 parts by weight of substrate; S2: First, pre-mix the coarse aggregate and fine aggregate in a mixer for 30-60 seconds, then add the cementitious material and stir for 60-90 seconds, then add the porous carrier loaded with induced bacteria and stir for 90-120 seconds, and finally add the pre-mixed mixture of substrate, water reducer and water and stir for 90-120 seconds, then pour, vibrate, cure and finish; Wherein, the substrate includes urea and calcium lactate, and the inducing bacteria includes Bacillus pasteurianus.

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