Preparation method of nutrient solution for willow orchid

Abstract

The invention discloses a preparation method of a nutrient solution for epilobium angustifolium, and relates to the technical field of epilobium angustifolium planting, and the preparation method comprises the following steps: staged formula design: according to physiological needs of the epilobium angustifolium in a germination stage, a growth stage and a flowering stage, respectively preparing nutrient mother liquor containing differential components, and biological-organic synergistic system construction: adding sterilized plant source organic matters and willow rhizosphere symbiotic microbial agents into the mother liquor, and carrying out stress resistance strengthening treatment.The method has the advantages that a differential formula is designed according to the physiological characteristics of the willow in the germination period, the growth period and the flowering period, and root development is promoted through high-phosphorus and seaweed extracts in the germination period; in the growing period, the molar ratio of nitrogen to potassium is 1: 1.2, and humic acid is used for enhancing the stem leaf and water-retaining property; in the flowering stage, high-potassium low-nitrogen and sodium molybdate are used for improving pollen viability. The nutrient absorption efficiency is remarkably optimized through staged design, and experiments prove that the germination rate is increased by 28% or more, the flowering phase is prolonged by 18 days, and the flower bud falling-off rate is reduced by 40% or more.

Description

Technical Field

[0001] The invention relates to the technical field of angustifolia planting, and in particular to a method for preparing an angustifolia nutrient solution. Background Art

[0002] Current plant nutrient solution technologies generally suffer from formula overgeneralization, making it difficult to meet the physiological needs of specific plants. This is particularly true for Epilobium cultivation, where existing solutions often use a uniform formulation without optimizing for the differentiated nutritional needs of Epilobium during germination, growth, and flowering stages. Some technologies rely on organic materials such as human and animal feces, which can easily introduce pathogens and pose high health risks. Furthermore, the lack of stress-resistant ingredients for high-altitude, arid environments results in a low survival rate for Epilobium under these harsh conditions. Furthermore, the complex preparation process of traditional nutrient solutions requires low temperatures or dark storage, making it difficult to maintain long-term microbial activity and limiting their practical application.

[0003] For wild alpine plants such as Epilobium, existing technologies have not yet effectively solved the core problems of their growth stage adaptability, biological activity synergy and stress resistance enhancement. The general formula cannot meet the needs of root promotion in the budding period, stem strengthening in the growth period, and flower preservation in the flowering period; chemical chelating agents and single inorganic salt systems are prone to low absorption efficiency, and microbial agents are easily inactivated in high-salt environments. These defects restrict the application of Epilobium in large-scale planting and ecological restoration. It is urgent to develop a special nutrient solution preparation method that takes into account stage adaptability, safety and stability. To this end, we propose a nutrient solution preparation method for Epilobium. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for preparing a nutrient solution for Epilobium angustifolium.

[0005] To achieve the above object, the present invention provides the following technical solution: a method for preparing a nutrient solution for Epilobium angustifolium, comprising the following steps:

[0006] (1) Phased formula design: According to the physiological needs of Epilobium angustifolium during the budding, growing, and flowering stages, nutrient mother solutions containing differentiated components are prepared respectively;

[0007] (2) Construction of biological-organic synergistic system: adding sterilized plant-derived organic matter and epiphyllum rhizosphere symbiotic microbial agents to the mother liquor;

[0008] (3) Stress resistance strengthening treatment: adding betaine 0.1mg / L–0.3mg / L and potassium silicate 20mg / L–30mg / L;

[0009] (4) Stabilization process: Dissolve the calcium source and phosphate step by step, mix them, and add 0.05 wt% of citric acid as a stabilizer to achieve storage at room temperature and away from light for ≥6 months.

[0010] As a further solution of the present invention: in step (1):

[0011] Bud stage mother solution: The phosphorus content is 30% higher than that of the flowering stage mother solution, and 0.5g / L–1.0g / L of seaweed extract is added to promote root development;

[0012] Mother solution during the growth period: The molar ratio of nitrogen to potassium is 1:1.2, and humic acid 0.8g / L–1.2g / L is added to enhance the water retention and ion exchange capacity of the substrate;

[0013] Mother solution during flowering period: The molar ratio of potassium to nitrogen is 3:1, and sodium molybdate 0.5g / L–1.0μmol / L is added to improve pollen vitality and pollination success rate.

[0014] As a further solution of the present invention: in step (2):

[0015] The sterilized plant-derived organic matter is soybean meal fermentation liquid, which is sterilized at 121°C for 20 minutes;

[0016] The microbial agent contains arbuscular mycorrhizal (AM) fungal spores, and the addition amount is 10 4 CFU / mL–10 5 CFU / mL.

[0017] As a further solution of the present invention: the microbial agent is encapsulated in microcapsules, the capsule wall material is a sodium alginate-chitosan complex, and the bacteria are released by mechanically breaking the capsule wall during use.

[0018] As a further solution of the present invention: in step (3):

[0019] Adding 0.8 μmol / L–1.2 μmol / L of boric acid reduced the flower bud abscission rate by ≥40%;

[0020] Betaine works synergistically with potassium silicate to increase the survival rate of Epilobium angustifolium by 30–50% in a low temperature environment of –5°C.

[0021] As a further solution of the present invention: in step (3), betaine improves the cell osmotic pressure tolerance, and potassium silicate enhances the mechanical strength of the cell wall, and together they cope with the high-altitude arid environment.

[0022] As a further embodiment of the present invention, the stepwise dissolution operation in step (4) comprises: first dissolving calcium nitrate in deionized water, then dissolving potassium dihydrogen phosphate separately, and finally slowly mixing and stirring for 30 minutes.

[0023] As a further solution of the present invention: the AM fungal agent in step (2) is embedded in sodium alginate-chitosan microcapsules with a particle size of 100 μm-200 μm and a wall-breaking release pH value of 6.0-6.5.

[0024] As a further embodiment of the present invention, 0.8 μmol / L-1.2 μmol / L boric acid is added to the mother liquor at each stage, and the pH value of the mother liquor is 5.8-6.2 during the budding period, 6.0-6.5 during the growth period, and 6.2-6.8 during the flowering period.

[0025] As a further solution of the present invention: after the nutrient solution is stored at 25° C. in the dark for 8 months, the active ingredient retention rate is ≥95%, the angustifolia germination rate is increased by 28%, and the flowering period is extended by 18 days.

[0026] By adopting the above technical solution, compared with the prior art, the beneficial effects of the present invention are:

[0027] 1. This invention designs differentiated formulas based on the physiological characteristics of Epilobium angustifolium during its budding, growing, and flowering stages: During the budding stage, high phosphorus (30% higher than during the flowering stage) and seaweed extract promote root development; during the growing stage, a nitrogen-potassium molar ratio of 1:1.2 and humic acid enhance stem and leaf growth and water retention; during the flowering stage, high potassium and low nitrogen (potassium-nitrogen molar ratio of 3:1) and sodium molybdate enhance pollen vitality. This phased design significantly optimizes nutrient absorption efficiency, with experimental results demonstrating a 28% or greater increase in germination rate, an 18-day extension of the flowering period, and a 40% or greater reduction in bud drop.

[0028] 2. The present invention uses sterilized soybean meal fermentation liquid to replace human and animal feces to avoid pathogen contamination (sterilization at 121°C for 20 minutes), combined with microcapsule-embedded AM fungi (Glomus intraradices, spore volume 10 4 –10 5 CFU / mL), and the capsule wall material (sodium alginate-chitosan) protects the bacteria from inactivation in high-salt environments. Breaking the capsule wall releases the bacteria and precisely improves phosphorus absorption. This system achieves synergistic effects between organic matter and microorganisms, with an AM fungal survival rate of ≥92% and an active ingredient retention rate of ≥95%, addressing the health risks and inactivation issues of traditional technologies.

[0029] 3. This invention enhances cell osmotic regulation by adding betaine (0.1–0.3 mg / L) and potassium silicate (20–30 mg / L) to strengthen the mechanical strength of the cell wall. These synergistically enhance the survival rate of Epilobium angustifolium at -5°C by 30–50% in response to high-altitude drought conditions. Furthermore, the stepwise dissolution of a calcium source and phosphate (to prevent precipitation), the addition of a citric acid stabilizer (0.05 wt%), and a nitrogen-filled sealing process enable storage at room temperature and in the dark for eight months, overcoming the limitation of traditional nutrient solutions that require low-temperature storage and making them suitable for large-scale production and field applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a general flow chart of the preparation of an epilobium nutrient solution in an embodiment of the present invention;

[0031] Figure 2A detailed flow chart of the phased formulation design in the embodiment of the present invention;

[0032] Figure 3 A flow chart for constructing a biological-organic synergistic system in an embodiment of the present invention;

[0033] Figure 4 This is a flow chart of stress resistance strengthening treatment in an embodiment of the present invention;

[0034] Figure 5 Flow chart of the stabilization process and preservation in an embodiment of the present invention. DETAILED DESCRIPTION

[0035] The specific embodiments of the present invention will be further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0036] In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0037] Please see the attached Figure 1 -Attached Figure 5 The present invention provides a method for preparing a nutrient solution for Epilobium angustifolium, comprising the following steps:

[0038] (1) Phased formula design: According to the physiological needs of Epilobium angustifolium during the budding, growing, and flowering stages, nutrient mother solutions containing differentiated components are prepared respectively;

[0039] (2) Construction of biological-organic synergistic system: adding sterilized plant-derived organic matter and epiphyllum rhizosphere symbiotic microbial agents to the mother liquor;

[0040] (3) Stress resistance strengthening treatment: adding betaine 0.1mg / L–0.3mg / L and potassium silicate 20mg / L–30mg / L;

[0041] (4) Stabilization process: Dissolve the calcium source and phosphate step by step, mix them, and add 0.05 wt% of citric acid as a stabilizer to achieve storage at room temperature and away from light for ≥6 months.

[0042] In one embodiment of the present invention: in step (1):

[0043] Bud stage mother solution: The phosphorus content is 30% higher than that of the flowering stage mother solution, and 0.5g / L–1.0g / L of seaweed extract is added to promote root development;

[0044] Mother solution during the growth period: The molar ratio of nitrogen to potassium is 1:1.2, and humic acid 0.8g / L–1.2g / L is added to enhance the water retention and ion exchange capacity of the substrate;

[0045] Mother solution during flowering period: The molar ratio of potassium to nitrogen is 3:1, and sodium molybdate 0.5g / L–1.0μmol / L is added to improve pollen vitality and pollination success rate.

[0046] In one embodiment of the present invention: in step (2):

[0047] The sterilized plant-derived organic matter was soybean meal fermentation broth, sterilized at 121°C for 20 min;

[0048] The microbial inoculant contains arbuscular mycorrhizal (AM) fungal spores, added at a rate of 10 4 CFU / mL–10 5 CFU / mL.

[0049] In one embodiment of the present invention, the microbial agent is encapsulated in microcapsules, the capsule wall material is a sodium alginate-chitosan complex, and the bacteria are released by mechanically breaking the capsule wall during use.

[0050] In one embodiment of the present invention: in step (3):

[0051] Adding 0.8 μmol / L–1.2 μmol / L of boric acid reduced the flower bud abscission rate by ≥40%;

[0052] Betaine works synergistically with potassium silicate to increase the survival rate of Epilobium angustifolium by 30–50% in a low temperature environment of –5°C.

[0053] In one embodiment of the present invention: in step (3), betaine improves the cell osmotic pressure tolerance, and potassium silicate enhances the mechanical strength of the cell wall, and together they cope with the high-altitude drought environment.

[0054] In one embodiment of the present invention, the stepwise dissolution operation in step (4) comprises: first dissolving calcium nitrate in deionized water, then dissolving potassium dihydrogen phosphate separately, and finally slowly mixing and stirring for 30 minutes.

[0055] In one embodiment of the present invention: the AM fungal agent in step (2) is embedded in sodium alginate-chitosan microcapsules with a particle size of 100 μm-200 μm and a wall-breaking release pH value of 6.0-6.5.

[0056] In one embodiment of the present invention, 0.8 μmol / L-1.2 μmol / L boric acid is added to the mother liquor at each stage, and the pH value of the mother liquor is 5.8-6.2 during the budding stage, 6.0-6.5 during the growth stage, and 6.2-6.8 during the flowering stage.

[0057] In one embodiment of the present invention, after the nutrient solution is stored at 25° C. in the dark for 8 months, the active ingredient retention rate is ≥95%, the epilobium germination rate is increased by 28%, and the flowering period is extended by 18 days.

[0058] Example 1 (Applicable to high-altitude cold areas)

[0059] 1. Staged mother liquor preparation

[0060] Mother solution for germination period:

[0061] Take potassium dihydrogen phosphate (the proportion of P2O5 increases by 30% vs. the flowering period), add seaweed extract (0.8g / L, derived from Ascophyllum nodosum), dissolve in deionized water, and adjust the pH value to 5.9.

[0062] Growth stage mother liquor:

[0063] Prepared at a nitrogen-potassium molar ratio of 1:1.2 (ammonium nitrate + potassium sulfate), integrated with 1.0 g / L humic acid (derived from weathered coal), pH 6.2.

[0064] Flowering mother solution:

[0065] The potassium-nitrogen molar ratio was 3:1 (potassium sulfate + calcium nitrate), 0.8 μmol / L sodium molybdate was added, and the pH value was 6.5.

[0066] 2. Bio-organic synergistic system

[0067] Soybean meal fermentation broth:

[0068] Soybean meal was mixed with water at a ratio of 1:5, inoculated with Bacillus subtilis (fermented at 30°C for 72 h), and sterilized at 121°C for 20 min.

[0069] Microbial agents:

[0070] Glomus intraradices spores (2×10 4 CFU / mL), and embedded in sodium alginate-chitosan microcapsules (particle size 150 μm).

[0071] 3. Anti-adversity strengthening treatment

[0072] Betaine 0.25 mg / L (osmotic regulator) and potassium silicate 25 mg / L (cell wall strengthener) were added, and boric acid 1.0 μmol / L was added simultaneously.

[0073] 4. Stabilization process

[0074] Dissolve in steps: Calcium nitrate (dissolved in 40°C deionized water) and potassium dihydrogen phosphate (dissolved separately) are slowly mixed and stirred for 30 minutes.

[0075] Add 0.05 wt% citric acid and fill in the container in the dark.

[0076] 5. Effect verification

[0077] Storage stability: After storage at 25°C away from light for 8 months, the active ingredient retention rate is 96.3%.

[0078] Stress resistance: After being treated at -5℃ for 7 days, the survival rate of Epilobium angustifolium was 48% (only 15% in the control group).

[0079] Growth indicators: germination rate increased by 30%, flowering period extended by 19 days, and flower bud shedding rate reduced by 42%.

[0080] Example 2 (Scaled production optimization)

[0081] 1. Staged mother liquor preparation

[0082] Germination stage: Phosphorus content increased by 30% (monoammonium phosphate), seaweed extract 1.0 g / L (pH 6.0).

[0083] Growth period: nitrogen-potassium molar ratio 1:1.2 (urea + potassium chloride), humic acid 1.2 g / L (pH 6.4).

[0084] Flowering period: potassium-nitrogen molar ratio 3:1 (potassium nitrate + ammonium sulfate), sodium molybdate 1.0 μmol / L (pH 6.7).

[0085] 2. Bio-organic synergistic system

[0086] Soybean meal fermentation broth: Processed in a large-scale fermentation tank (500L), sterilized at 121℃ for 20 minutes.

[0087] Microbial agent: AM fungal spores (5×10 4 CFU / mL), and the microcapsule wall was broken by centrifugal shaking (3000 rpm, 10 min).

[0088] 3. Stress resistance strengthening

[0089] Betaine 0.3mg / L + potassium silicate 30mg / L + boric acid 1.2μmol / L.

[0090] 4. Stabilization process

[0091] The calcium source (calcium chloride) and phosphate (potassium hydrogen phosphate) are dissolved in steps, mixed, and then citric acid is added.

[0092] Microcapsule embedding process: preparation by spray drying method (inlet temperature 120℃, outlet temperature 60℃).

[0093] 5. Effect verification

[0094] Process efficiency: Single batch output increased to 1 ton, and dissolution time shortened to 25 minutes.

[0095] Storage performance: After 8 months, the survival rate of AM fungi was 92%, and the betaine retention rate was 94.5%.

[0096] Application effect: low temperature survival rate is 52%, flowering period is extended by 21 days, suitable for plateau greenhouse cultivation.

[0097] Example 3 (precise control of trace elements)

[0098] 1. Staged mother liquor preparation

[0099] Germination stage: Phosphorus content increased by 30% (potassium dihydrogen phosphate), seaweed extract 0.5g / L (pH 5.8).

[0100] Growth period: nitrogen-potassium molar ratio 1:1.2 (potassium nitrate + ammonium sulfate), humic acid 0.8 g / L (pH 6.0).

[0101] Flowering period: potassium-nitrogen molar ratio 3:1 (potassium dihydrogen phosphate + calcium nitrate), sodium molybdate 0.5 μmol / L (pH 6.8).

[0102] 2. Bio-organic synergistic system

[0103] Soybean meal fermentation broth: pre-treated with enzymatic hydrolysis (cellulase 50 U / g, hydrolysis at 50°C for 4 h) and sterilized before use.

[0104] Microbial agents: AM fungi (Rhizophagusirregularis, 1×10 5 CFU / mL), microcapsule wall breaking pH 6.3.

[0105] 3. Stress resistance strengthening

[0106] Betaine 0.1mg / L + potassium silicate 20mg / L + boric acid 0.8μmol / L (precisely controlled concentration).

[0107] 4. Stabilization process

[0108] Dissolve calcium nitrate and potassium dihydrogen phosphate step by step, mix and let stand in the dark to defoam.

[0109] The amount of citric acid added is 0.05 wt%, and the bottle is sealed with nitrogen after filling.

[0110] 5. Effect verification

[0111] Ingredient stability: After 8 months, the molybdenum element retention rate is 97.1%, and no boric acid crystals are precipitated.

[0112] Physiological indicators: pollen vitality increased by 35%, and the pollination success rate was 88% (70% in the control group).

[0113] Environmental adaptability: Under drought stress (soil moisture content 15%), the biomass of Epilobium angustifolium increased by 40%.

[0114] Specifically, a differentiated formula was designed based on the physiological characteristics of Epilobium truncatum during its budding, growing, and flowering stages: During the budding stage, high phosphorus (30% higher than during the flowering stage) and seaweed extract promote root development; during the growing stage, a nitrogen-potassium molar ratio of 1:1.2 and humic acid enhance stem and leaf capacity and water retention; during the flowering stage, high potassium and low nitrogen (potassium-nitrogen molar ratio of 3:1) and sodium molybdate enhance pollen vitality. This phased design significantly optimizes nutrient absorption efficiency, with experimental results demonstrating a 28% or greater increase in germination rate, an 18-day extension of the flowering period, and a 40% or greater reduction in bud drop.

[0115] Specifically, sterilized soybean meal fermentation liquid was used instead of human and animal feces to avoid pathogen contamination (sterilized at 121°C for 20 minutes), combined with microcapsulated AM fungi (Glomus intraradices, spore volume 10 4 –10 5 CFU / mL), and the capsule wall material (sodium alginate-chitosan) protects the bacteria from inactivation in high-salt environments. Upon release from the capsule wall, phosphorus absorption is precisely enhanced. This system achieves synergistic effects between organic matter and microorganisms, achieving an AM fungal survival rate of ≥92% and an active ingredient retention rate of ≥95%, addressing the hygienic risks and inactivation of microbial agents associated with traditional technologies.

[0116] Specifically, the addition of betaine (0.1–0.3 mg / L) enhances cellular osmotic regulation and potassium silicate (20–30 mg / L) strengthens the mechanical strength of the cell wall, synergistically increasing the survival rate of Epilobium angustifolium by 30–50% at -5°C in response to high-altitude drought conditions. Furthermore, the stepwise dissolution of a calcium source and phosphate (to prevent precipitation), the addition of a citric acid stabilizer (0.05 wt%), and a nitrogen-filled, sealed process allow for eight months of storage at room temperature in the dark, overcoming the limitations of traditional nutrient solutions that require low-temperature storage and making them suitable for large-scale production and field applications.

[0117] Although the present invention is disclosed above in terms of preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present invention. Therefore, any modification, equivalent change and modification made to the above embodiments in accordance with the technical essence of the present invention without departing from the content of the technical solution of the present invention shall be construed as follows:

[0118] All of them fall within the protection scope defined by the claims of the present invention.

Claims

1. A method for preparing a nutrient solution for Epilobium angustifolium, characterized in that: The following steps are involved: (1) Phased formula design: According to the physiological needs of Epilobium angustifolium during the budding, growing, and flowering stages, nutrient mother solutions containing differentiated components are prepared respectively; (2) Construction of biological-organic synergistic system: adding sterilized plant-derived organic matter and epiphyllum rhizosphere symbiotic microbial agents to the mother liquor; (3) Stress resistance strengthening treatment: adding betaine 0.1mg / L–0.3mg / L and potassium silicate 20mg / L–30mg / L; (4) Stabilization process: Dissolve the calcium source and phosphate step by step, mix them, and add 0.05 wt% of citric acid as a stabilizer to achieve storage at room temperature and away from light for ≥6 months.

2. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: In the step (1): Bud stage mother solution: The phosphorus content is 30% higher than that of the flowering stage mother solution, and 0.5g / L–1.0g / L of seaweed extract is added to promote root development; Mother solution during the growth period: The molar ratio of nitrogen to potassium is 1:1.2, and humic acid 0.8g / L–1.2g / L is added to enhance the water retention and ion exchange capacity of the substrate; Mother solution during flowering period: The molar ratio of potassium to nitrogen is 3:1, and sodium molybdate 0.5g / L–1.0μmol / L is added to improve pollen vitality and pollination success rate.

3. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: In the step (2): The sterilized plant-derived organic matter is soybean meal fermentation liquid, which is sterilized at 121°C for 20 minutes; The microbial agent contains arbuscular mycorrhizal (AM) fungal spores, and the addition amount is 10 4 CFU / mL–10 5 CFU / mL.

4. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 3, wherein: The microbial agent is embedded in microcapsules, the capsule wall material is a sodium alginate-chitosan complex, and the bacteria are released by mechanically breaking the capsule wall during use.

5. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: In the step (3): Adding 0.8 μmol / L–1.2 μmol / L of boric acid reduced the flower bud abscission rate by ≥40%; Betaine works synergistically with potassium silicate to increase the survival rate of Epilobium angustifolium by 30–50% in a low temperature environment of –5°C.

6. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, characterized in that: In step (3), betaine improves the cell's osmotic pressure tolerance, and potassium silicate enhances the mechanical strength of the cell wall, and together they cope with the high-altitude drought environment.

7. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: The stepwise dissolution operation in step (4) includes: first dissolving calcium nitrate in deionized water, then dissolving potassium dihydrogen phosphate separately, and finally slowly mixing and stirring for 30 minutes.

8. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: The AM fungal agent in step (2) is embedded in sodium alginate-chitosan microcapsules with a particle size of 100 μm-200 μm and a pH value of 6.0-6.5 when the microcapsules are broken.

9. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: 0.8 μmol / L–1.2 μmol / L boric acid was added to the mother liquor at each stage, and the pH value of the mother liquor was 5.8–6.2 during the budding stage, 6.0–6.5 during the growth stage, and 6.2–6.8 during the flowering stage.

10. The method for preparing a nutrient solution for Epilobium angustifolium according to claim 1, wherein: After the nutrient solution is stored at 25° C. in the dark for 8 months, the active ingredient retention rate is ≥95%, the angustifolia germination rate is increased by 28%, and the flowering period is extended by 18 days.

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