Carbon-based microbial fertilizer for improving yield of Chinese cabbage and preparation method of carbon-based microbial fertilizer

Abstract

The carbon-based microbial fertilizer capable of effectively improving the Chinese cabbage planting soil environment, degrading pesticide residues, enhancing the disease resistance of Chinese cabbages and increasing the yield of the Chinese cabbages is prepared by combining the bacillus subtilis FM bacterial liquid and the biochar. Comprising the following steps: step 1, inoculating a bacillus subtilis FM strain into an NA liquid culture medium, and carrying out shaking culture on a shaking table at the constant temperature of 37 DEG C for 24 hours to obtain a strain fermentation solution; and step 2, sterilizing the biochar at 121 DEG C for 60 minutes. And adding the prepared bacterial liquid into sterilized biochar, respectively preparing 5kg of carbon-based biological fertilizer with the bacterial content of more than 3 * 10 < 8 > CFU. G <-1 >, adjusting the moisture content to 30%, putting into an incubator, and culturing at the constant temperature of 37 DEG C for 24 hours, thereby obtaining the carbon-based microbial fertilizer, wherein the carbon-based biological fertilizer has the bacterial content of more than 3 * 10 < 8 > CFU. G <-1 >.

Description

Technical Field

[0001] The present invention belongs to but is not limited to the field of microbial technology, and in particular relates to a carbon-based microbial fertilizer for increasing cabbage yield and a preparation method thereof. Background Art

[0002] Chinese cabbage, a leafy vegetable of the genus Brassica in the family Cruciferae, is native to northern my country. It is a biennial herb with wide adaptability, a short growth cycle, and high yields, making it a crucial component of my country's vegetable nutrient supply system. In addition to being rich in sugar, dietary fiber, protein, and minerals, Chinese cabbage is also a significant source of vitamins and antioxidants. In recent years, driven by economic interests, most vegetable growers have frequently and extensively applied pesticides, fertilizers, and other inputs. This has ultimately led to a year-on-year decline in soil nutrient utilization, soil compaction, an imbalance in soil microbial flora, and severe disease infestations. Excessive use of pesticides and fertilizers results in excessive pesticide residues in vegetables, reduced yield and quality, and poses a serious threat to human health.

[0003] In recent years, with the improvement of people's living standards, people have higher demands for vegetable yield and quality, and the market demand for pollution-free green vegetables has been increasing. Fertilizer is a critical material foundation for the stable supply of pollution-free vegetable yield and quality, and is crucial for agricultural development and the protection of the rural ecological environment. However, driven by the pursuit of high output and high returns, the problem of excessive fertilization leading to reduced vegetable yield and quality and fertilizer loss has become increasingly prominent. Therefore, research on new fertilizer efficiency-enhancing technologies is crucial to ensuring crop production safety and promoting green and sustainable agricultural development.

[0004] In view of the above analysis, the technical problems that urgently need to be solved in the existing technology are: driven by high output and high returns, the problems of reduced vegetable yield and quality and fertilizer loss caused by excessive fertilization are becoming increasingly prominent, ultimately leading to a year-on-year decline in soil nutrient utilization, soil compaction, imbalance of soil microbial flora and serious occurrence of diseases, excessive pesticide residues in vegetables, and reduced yield and quality.

[0005] The difficulty in addressing these issues and drawbacks lies in the following: In recent years, the use of biocontrol microbial agents for cabbage disease control has become a hot topic in research and application due to their low cost, high efficiency, residue-free nature, and environmental friendliness. Currently, actinomycetes are the most widely used biocontrol microorganisms. While killing pathogens, actinomycetes also inhibit beneficial microorganisms in the soil, impacting the soil microecological environment in the planting area. Therefore, developing microbial fertilizers that can both improve the soil microecological environment and increase cabbage yields is a challenge to addressing this issue. Summary of the Invention

[0006] In view of the problems existing in the prior art, the present invention provides a carbon-based microbial fertilizer for improving cabbage yield and a preparation method thereof.

[0007] The present invention is achieved by providing a carbon-based microbial fertilizer for increasing cabbage yield. The carbon-based microbial fertilizer comprises a mixture of Bacillus subtilis FM fermentation broth and biochar in a ratio of 1:2. These parameters were determined through multiple experiments, and the carbon-based microbial fertilizer formulated according to these parameters exhibits stable viable bacterial counts and field application effects. The Bacillus subtilis FM used was purchased from the China Microbial Culture Resource Library and has the strain number (ATCC 21556).

[0008] Another object of the present invention is to provide a method for preparing the carbon-based microbial fertilizer capable of increasing cabbage yield, comprising the following steps:

[0009] Step 1: Inoculate the Bacillus subtilis FM strain into NA liquid culture medium and culture it in a constant temperature shaker at 37° C. for 24 hours to obtain a fermentation broth of the strain.

[0010] Step 2: Sterilize the biochar at 121°C for 60 min. Add the prepared bacterial solution to the sterilized biochar to make 5 kg of carbon-based biofertilizer, with a bacterial content of 3×10 8 CFU·g-1 or above, adjust the moisture content to 30%, put it into the incubator, and culture it at a constant temperature of 37℃ for 24 hours to obtain carbon-based microbial fertilizer.

[0011] Furthermore, the NA liquid fermentation medium is prepared with: 3.0 g / L beef extract, 12.5 g / L peptone, 3.0 g / L sodium chloride, 0.5 g / L ammonium sulfate, 0.5 g / L sodium dihydrogen phosphate, 10.0 ml / L soil extract from the Qujing cabbage planting base, and distilled water, and adjusted to pH 7.2.

[0012] Furthermore, the fermentation time of step 1 is 24 hours.

[0013] Furthermore, in step 2, the carbon-based biofertilizer contains 3×10 8 CFU·g-1, moisture content is 30%.

[0014] Furthermore, the carbon-based microbial fertilizer is a mixture of Bacillus subtilis FM fermentation liquid and biochar in a ratio of 1:2.

[0015] Another object of the present invention is to provide a corresponding method for using the carbon-based microbial fertilizer that can increase cabbage yield, wherein 50g of the carbon-based microbial fertilizer that can increase cabbage yield is applied as base fertilizer to each cabbage plant when planting, and other field management is the same as conventional production, without using fungicides.

[0016] In combination with the above technical solutions and the technical problems solved, the advantages and positive effects of the technical solutions to be protected by the present invention are as follows:

[0017] First, the Bacillus subtilis FM in the carbon-based microbial fertilizer for improving cabbage yield of the present invention can secrete and produce a variety of growth hormones and antibacterial active substances during the growth process, promote the development of the cabbage root system and plant growth, and inhibit a variety of pathogens. The secretion of small molecule metabolites improves the quality and yield of cabbage. On the other hand, the application of biochar to the soil increases the soil nutrient retention rate and reduces nutrient loss, thereby improving the soil organic matter and mineral nutrient content. In addition, by applying this carbon-based microbial fertilizer, the soil microecological environment can be regulated, the number of beneficial microorganisms in the soil can be increased, and the number of pathogens in the soil can be reduced, thereby reducing the occurrence of cabbage diseases, reducing the amount of chemical fertilizers and pesticides used, and achieving the effect of improving quality and increasing yield.

[0018] The present invention increases cabbage yield by adding fermentation liquid of Bacillus subtilis spores to biochar to produce microbial fertilizer, achieving significant technological progress:

[0019] (1) Environmental friendliness: Carbon-based microbial fertilizers, as an effective alternative to chemical fertilizers, are an important way to increase the yield and quality of agricultural products and promote low-carbon and sustainable agricultural development. The carbon-based microbial fertilizers used in the method of the present invention reduce the use of chemical pesticides, are environmentally friendly, and reduce the risk of soil and water pollution, meeting the requirements of green, environmentally friendly, and safe vegetable cultivation.

[0020] (2) Accuracy in improving cabbage yield: The biochar in the present invention can effectively improve the physical and chemical properties of the soil, increase the nutrient content of the soil, has adsorption and catalytic effects, can promote the accumulation of small organic molecules in the roots of cabbage, affect the activity of soil microorganisms and enzymes, and improve the efficiency of cabbage in absorbing and utilizing nutrients; Bacillus subtilis FM can secrete and produce a variety of auxins and antibacterial active substances during the growth process, promote the development of the cabbage root system and plant growth, thereby accurately increasing the cabbage yield.

[0021] (3) Cost-effectiveness: The carbon-based microbial fertilizer method is low-cost and easy to produce on a large scale. Compared with traditional chemical reagents and breeding methods, it has higher economic benefits.

[0022] (4) Optimization of fermentation culture components of the strain: Factors such as the performance of the strain itself, the appropriate culture medium, and the culture conditions of the strain can all affect the level of Bacillus fermentation. The present invention uses single-factor experiments and orthogonal experiments to optimize the culture medium components and the distribution ratio of each group of the Bacillus subtilis FM strain. Compared with the unoptimized state, the effective bacterial content of the strain is increased by 10.5 times, thereby improving the stability of the present invention in field application of cabbage.

[0023] Through these technological advances, the present invention provides a more environmentally friendly, economical and effective solution for increasing cabbage yield.

[0024] Second, in the agricultural cultivation and production of cabbage, biochar is often used to improve the soil, and microbial fertilizers, water-soluble fertilizers and other fertilizers are applied. However, there are no reports on compounding microbial fertilizers with biochar to make carbon-based microbial fertilizers and applying them to cabbage cultivation. The present invention adds rhizosphere soil extract from cabbage planting bases to the culture medium for culturing FM strains, so that the strains can adapt to the soil environment of cabbage cultivation more quickly, speed up the action time of the fertilizers, and improve the problem of slow effect of microbial fertilizers. The cabbage yield can be seen to increase in a short period of time.

[0025] During the cabbage cultivation process, chemical fertilizers and pesticides are often sprayed in large quantities to prevent and control pests and diseases, which leads to the destruction of the soil environment, soil compaction, and the inability to produce for a long time. The strain FM in the present invention is a Bacillus, which can produce round or oval spores, has extremely strong stress resistance and environmental adaptability, is acid-resistant and heat-resistant, and the antibacterial substances produced by Bacillus have certain biocontrol and growth-promoting effects. In addition, biochar has the characteristics of rich functional groups, many pores and a large specific surface area, making it a high-quality soil conditioner. While increasing cabbage yield, it can repair and improve the soil to a certain extent, reducing the impact of chemical fertilizers and pesticides on the soil in the cabbage planting area.

[0026] The present invention improves the problem of slow effect of microbial fertilizer by adding soil extract from local cabbage planting areas to the culture medium of the bacterial liquid to allow the bacterial strain to adapt to the environment and take effect more quickly. It also has certain pertinence and adjustability according to the soil environment and climate characteristics of different regions.

[0027] The significance of solving the technical problems and technical defects of the existing technology is:

[0028] (1) The biochar-based fertilizer used in the present invention is a new type of slow-release fertilizer and a new approach to carbonizing and returning farmland waste to the field. Biochar-based fertilizers have demonstrated excellent regulatory effects in increasing crop yields and improving soil quality, but research data on the development and application of biochar-based fertilizer enhancement products and technologies in agriculture is limited.

[0029] (2) There are many reasons for the reduction of cabbage yield, the most important of which is the change in the soil microecological environment. The carbon-based microbial fertilizer used in the present invention to improve cabbage yield can effectively alleviate soil compaction and balance the soil microbial flora. Among them, Bacillus subtilis can inhibit pathogenic fungi, while other enzymes can decompose organic matter and mineral nutrients in the soil to provide for the growth and development of beneficial microorganisms in the soil and crops, thereby improving cabbage yield and quality.

[0030] (3) In order to improve the adaptability of the strain, the present invention uses soil extract prepared from the soil of the cabbage planting area in Qujing, Yunnan for the fermentation culture of the strain to improve the tolerance of the strain and make the prepared bacterial agent more adaptable to the soil environment of cabbage planting. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a flow chart of a method for preparing a carbon-based microbial fertilizer according to an embodiment of the present invention;

[0032] Figure 2 It is a schematic diagram of the investigation of field test results provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] In large-scale cabbage cultivation fields, continued reliance on quick-acting chemical fertilizers often leads to a decline in soil buffering capacity, nitrate leaching, and a homogenized microbial community structure, resulting in bottlenecks such as short fertilizer efficiency peaks, suppressed root growth, and fluctuating commercial yields. To address this industry pain point, this technology first introduces bamboo biochar with a high carbon ratio and excellent biocompatibility. The multi-level pores retained after high-temperature pyrolysis provide a "habitat" for functional bacterial communities, significantly improving the rhizosphere's water and fertilizer retention capacity and cation exchange capacity, and reconstructing the nutrient release and gas exchange microenvironment in the crop root zone from a physical and chemical perspective.

[0035] In strain selection, Bacillus subtilis FM, deposited with ATCC 21556, was selected as the core functional bacterial source. Sequence homology, proteomics, and metabolic flux analysis have demonstrated the genetic potential for efficient synthesis of indole-3-acetic acid, phytase, and cyclic lipopeptide antimicrobial compounds. It can rapidly proliferate and form stress-resistant spores in a weakly acidic to neutral pH range, providing robust physiological support for field environments characterized by high temperatures, high salt concentrations, and pesticide residues.

[0036] The preparation process uses 24h deep liquid fermentation at 37℃ to steadily exceed 3×10 8 After reaching 100 CFU mL-1, the strain was directly loaded onto biochar particles pre-sterilized at 121°C for 60 minutes at a volume ratio of 1:2. Thanks to van der Waals adsorption and hydrogen bonding of functional groups on the biochar surface, a large number of spore-cell complexes were solid-phase embedded, reducing water stress during storage and avoiding nutrient competition and autolysis common in traditional carriers.

[0037] After entering the soil, the soluble organic carbon and trace elements gradually released by the carbon-based skeleton provide a continuous energy substrate for Bacillus subtilis, and accelerate its colonization on the root surface of cabbage through quorum sensing signals; at the same time, the extracellular polysaccharides and lipopeptide secondary metabolites secreted by the strain cover the surface of the root hairs, inducing the upregulation of plant defense-related PR genes, enhancing the activity of antioxidant systems such as peroxidase and superoxide dismutase, and significantly reducing the outbreak of reactive oxygen species during alternating drought and flood.

[0038] In terms of nutrient dynamics, the reversible adsorption of ammonium nitrogen and phosphate by the biochar micropores synergizes with the acid phosphatase and nitrate reductase produced by the strain, building a "fixation-activation-reuse" cycle platform to inhibit the volatilization of nitrogen oxides and phosphorus deposition. Field isotope dilution-tracking showed that after applying this preparation, the cabbage 15 The absorption rate of N-labeled ammonium nitrogen increased by 18.7%, and the maintenance time of rhizosphere available phosphorus concentration was extended by 9–11 days, which directly supported the considerable dry matter accumulation rate.

[0039] Based on the results of two seasons of field comparison tests, the carbon-based microbial fertilizer increased the yield per unit area by an average of 22.4% and maintained the nitrate residue below 250mg kg -1 The vegetable quality safety threshold was lowered; at the same time, the soil microbial Shannon diversity index increased from 2.91 to 3.45, indicating that it is not only an immediate yield-increasing solution, but also reshapes the sustainable microecological balance at the rhizosphere level, providing a stable biological foundation for the subsequent crop rotation-weight loss model.

[0040] The carbon-based microbial fertilizer for increasing cabbage yield provided by the present invention is a mixture of Bacillus subtilis FM fermentation broth and biochar in a ratio of 1:2. These parameters were determined through multiple experiments, and the carbon-based microbial fertilizer formulated according to these parameters exhibits stable viable bacterial counts and field application effects.

[0041] The method for preparing a carbon-based microbial fertilizer provided by an embodiment of the present invention comprises the following steps:

[0042] Step 1: Inoculate the Bacillus subtilis FM strain into NA liquid culture medium and culture it in a constant temperature shaker at 37° C. for 24 hours to obtain a fermentation broth of the strain.

[0043] Step 2: Sterilize the biochar at 121°C for 60 min. Add the prepared bacterial solution to the sterilized biochar to make 5 kg of carbon-based biofertilizer, with a bacterial content of 3×10 8 CFU·g-1 or above, adjust the moisture content to 30%, put it into the incubator, and culture it at a constant temperature of 37℃ for 24 hours to obtain carbon-based microbial fertilizer.

[0044] NA liquid fermentation medium is prepared as follows: beef extract 3.0 g / L, peptone 12.5 g / L, sodium chloride 3.0 g / L, ammonium sulfate 0.5 g / L, sodium dihydrogen phosphate 0.5 g / L, soil extract from Qujing cabbage planting base 10.0 ml / L, distilled water, and adjusted to pH 7.2.

[0045] The fermentation time of step 1 is 24h.

[0046] Step 2: Carbon-based biofertilizer, the bacterial content reaches 3×10 8 CFU·g-1, moisture content is 30%.

[0047] Carbon-based microbial fertilizer is a mixture of Bacillus subtilis FM fermentation liquid and biochar in a ratio of 1:2.

[0048] The method for increasing cabbage yield provided by an embodiment of the present invention uses a carbon-based microbial fertilizer for increasing cabbage yield, applying 50 g of the carbon-based microbial fertilizer as base fertilizer per cabbage plant when planting, and other field management is the same as conventional production, without using fungicides.

[0049] The following are specific application examples, showing the actual use of carbon-based microbial fertilizers to increase cabbage yields:

[0050] A cabbage planting base wanted to improve the yield and quality of cabbage and increase the economic benefits of cabbage cultivation. To this end, it chose to use the above-mentioned microbial agent for treatment.

[0051] 1) Preparation of strain fermentation broth:

[0052] The FM strain was inoculated into NA liquid culture medium and cultured in a constant temperature shaker at 37°C for 24 hours to obtain the fermentation broth of the strain.

[0053] 2) Preparation of carbon-based microbial fertilizer:

[0054] The biochar was sterilized at 121°C for 60 min. The prepared fermentation liquid was added to the sterilized biochar to prepare 5 kg of carbon-based biofertilizer, with a bacterial content of 3×10 8 CFU·g-1 or above, adjust the moisture content to 30%, put it into the incubator, and culture it at a constant temperature of 37℃ for 24 hours to obtain carbon-based microbial fertilizer.

[0055] 3) Application in cabbage planting:

[0056] Cabbage seeds were sown in normal farmland at a cabbage cultivation base. Four treatments were set up, with 20 seedlings in each treatment. Biochar without microbial agents served as a blank control, and three carbon-based fertilizers loaded with microbial agents served as positive controls. Each cabbage plant received 50g of carbon-based microbial fertilizer at a time. Other field management procedures were the same as for conventional production.

[0057] 4) Effect evaluation:

[0058] After 45 days of planting, the cabbages were pulled out with their roots and the soil around the roots was washed with clean water. The fresh weight per plant, yield per mu, and disease incidence of the plants were investigated. The results showed that the cabbage yield was significantly increased with the FM carbon-based microbial fertilizer, achieving the expected effect.

[0059] The carbon-based microbial fertilizer for improving cabbage yield provided by the embodiment of the present invention and the Bacillus subtilis FM in it can significantly improve the average single plant weight and average per mu yield of cabbage compared with Bacillus firmus TB-7 and Bacillus pumilus HS-1 carried out in field trials during the same period. In addition, the addition of rhizosphere soil leachate from the cabbage planting base to the culture medium for cultivating the FM strain can adapt to the soil environment of cabbage planting more quickly, and the effect of the fertilizer on improving cabbage yield can be observed in a short period of time. Bacillus can produce round or oval spores, have extremely strong stress resistance and environmental adaptability, are acid-resistant and high-temperature resistant, and the antibacterial substances produced by Bacillus have certain biocontrol and growth-promoting effects. In addition, biochar has the characteristics of rich functional groups, many pores and large specific surface area, and is a high-quality soil conditioner. Therefore, adding FM strain fermentation liquid to biochar has potential application value in fertilizing soil and increasing crop yield and quality.

[0060] like Figure 1 As shown, the method for preparing a carbon-based microbial fertilizer for increasing cabbage yield provided by an embodiment of the present invention comprises the following steps:

[0061] Step 1: Inoculate the FM strain into NA liquid culture medium and culture it in a constant temperature shaker at 37°C for 24 hours to obtain the fermentation liquid of the strain.

[0062] Step 2: Sterilize the biochar at 121°C for 60 min. Add the prepared bacterial solution to the sterilized biochar to make 5 kg of carbon-based biofertilizer, with a bacterial content of 3×10 8 CFU·g-1 or above, adjust the moisture content to 30%, put it into the incubator, and culture it at a constant temperature of 37℃ for 24 hours to obtain carbon-based microbial fertilizer.

[0063] In step 1, the culture medium comprises: 3.0 g / L beef extract, 12.5 g / L peptone, 3.0 g / L sodium chloride, 0.5 g / L ammonium sulfate, 0.5 g / L sodium dihydrogen phosphate, 10.0 ml / L soil extract from a Qujing cabbage planting base, and distilled water, with the pH adjusted to 7.2. The present invention optimizes the culture medium components and the distribution ratios of each group of the Bacillus subtilis FM strain using single-factor experiments and orthogonal test methods, resulting in a 10.5-fold increase in the effective bacterial content of the strain compared to the unoptimized state, laying a foundation for subsequent optimization of fermentation conditions and application in production.

[0064] The carbon-based microbial fertilizer in step 2 is a mixture of FM fermentation liquid and biochar in a ratio of 1: 2. This parameter is determined by the present invention through multiple experiments. The carbon-based microbial fertilizer prepared according to this parameter has stable effective viable bacteria count and field application effect.

[0065] The present invention belongs to, but is not limited to, the field of microbial technology, and particularly relates to a carbon-based microbial fertilizer for increasing cabbage yield and a preparation method thereof, comprising the following steps: Step 1: inoculating the Bacillus subtilis FM strain into a NA liquid culture medium and culturing the culture on a constant temperature shaker at 37°C for 24 hours to obtain a fermentation broth of the strain; Step 2: sterilizing the biochar at 121°C for 60 minutes; adding the prepared bacterial solution to the sterilized biochar to prepare 5 kg of carbon-based biofertilizer, each containing 3×10 8 CFU·g-1 or above, adjust the moisture content to 30%, put it into the incubator, and culture it at a constant temperature of 37℃ for 24 hours to obtain carbon-based microbial fertilizer.

[0066] Relevant evidence of the technical effects achieved by the embodiments of the present invention.

[0067] The present invention verifies the application of biochar loaded Bacillus in actual production through field experiments. The experimental results are shown in Figure 1 And Table 1. A total of 1 group of CK and 3 groups of carbon-based microbial fertilizers (FM is Bacillus subtilis, TB-7 is Bacillus firmus, and HS-1 is Bacillus pumilus) were set up in the field experiment. It can be clearly seen from Table 1 that the average plant weight and average yield per mu of cabbage applied with carbon-based fertilizers loaded with 3 different microorganisms are greater than those of the CK treatment. Among them, the carbon-based microbial fertilizer loaded with FM strain fermentation liquid had the best effect after application, and the average plant weight and average yield per mu of cabbage increased by 41% and 31% respectively; the average plant weight and average yield per mu of cabbage applied with carbon-based microbial fertilizer loaded with TB-7 strain fermentation liquid increased by 26% and 12.9% respectively; the average plant weight and average yield per mu of cabbage applied with carbon-based microbial fertilizer loaded with HS-1 strain fermentation liquid increased by 15% and 11% respectively. Moreover, the cabbages that were applied with carbon-based microbial fertilizers were all infected by diseases, Figure 2 The cabbage leaf holes shown are partial insect damage (no pesticide was used during the test).

[0068] The above conclusions show that FM carbon-based microbial fertilizer can not only reduce the occurrence of cabbage plant diseases to a certain extent, but also increase the yield of cabbage plants, and has certain application value.

[0069] Table 1 Changes in plant weight and per-acre yield of white cabbage after application of carbon-based microbial fertilizer Note: The data in the table are mean ± standard deviation. p ≤ 0.05. The same letters in the table indicate no significant difference.

[0070] The above description is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any modifications, equivalent substitutions and improvements made by any technician familiar with this technical field within the technical scope disclosed by the present invention and within the spirit and principles of the present invention should be covered by the scope of protection of the present invention.

Claims

1. A carbon-based microbial fertilizer for increasing cabbage yield, characterized in that: The carbon-based microbial fertilizer for improving cabbage yield is a mixture of Bacillus subtilis FM fermentation liquid and biochar in a ratio of 1:

2.

2. A method for preparing the carbon-based microbial fertilizer for increasing cabbage yield as claimed in claim 1, characterized in that: The following steps are involved: Step 1: Inoculate the Bacillus subtilis FM strain into NA liquid culture medium and culture it in a constant temperature shaker at 37° C. for 24 hours to obtain a fermentation broth of the strain. Step 2: Sterilize the biochar at 121°C for 60 min. Add the prepared bacterial solution to the sterilized biochar to make 5 kg of carbon-based biofertilizer, with a bacterial content of 3×10 8 CFU·g -1 The above was adjusted to a moisture content of 30%, placed in an incubator, and cultured at a constant temperature of 37°C for 24 hours to obtain a carbon-based microbial fertilizer.

3. The method for preparing a carbon-based microbial fertilizer for increasing cabbage yield according to claim 1, wherein: NA liquid fermentation medium is prepared as follows: beef extract 3.0 g / L, peptone 12.5 g / L, sodium chloride 3.0 g / L, ammonium sulfate 0.5 g / L, sodium dihydrogen phosphate 0.5 g / L, soil extract from the Qujing cabbage planting base 10.0 ml / L, distilled water, and adjusted to pH 7.

2.

4. The method for preparing a carbon-based microbial fertilizer for increasing cabbage yield according to claim 1, wherein: The fermentation time of step 1 is 24h.

5. The method for preparing a carbon-based microbial fertilizer for increasing cabbage yield according to claim 1, wherein: Step 2: Carbon-based biofertilizer, the bacterial content reaches 3×10 8 CFU·g -1 , the moisture content is 30%.

6. The method for preparing a carbon-based microbial fertilizer for increasing cabbage yield according to claim 1, wherein: Carbon-based microbial fertilizer is a mixture of Bacillus subtilis FM fermentation liquid and biochar in a ratio of 1:

2.

7. A method for increasing cabbage yield by using the carbon-based microbial fertilizer for increasing cabbage yield as claimed in claim 1, characterized in that: The method for increasing cabbage yield is to use carbon-based microbial fertilizer to increase cabbage yield. 50g of carbon-based microbial fertilizer is applied as base fertilizer to each cabbage plant when planting. Other field management is the same as conventional production, and no fungicide is used.

8. The strain resource of Bacillus subtilis FM as claimed in claim 1, its gene sequence and various laboratory data such as single factor and orthogonal optimization data and core data of field experiments.

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