Methylobacterium culture equipment

Abstract

The invention relates to the technical field of culture medium mixing, in particular to methylobacterium culture equipment which comprises a fermentation tank and a stirring mechanism, and the fermentation tank comprises a tank body, a cover body and a rotary driver used for driving the stirring mechanism; the stirring mechanism comprises a main shaft and a stirring rod sleeved on the main shaft, and the main shaft is in transmission connection with the driving end of the rotary driver; stirring blades are rotationally arranged on the stirring rod; an auxiliary control mechanism for controlling the stirring blades to rotate is arranged in the tank body; when the stirring blades stop rotating and synchronously rotate along with the stirring rod, the stirring mechanism is in a first stirring state; and when the stirring blades synchronously rotate along with the stirring rod and simultaneously rotate, the stirring mechanism is in a second stirring state. The double-stirring-state function of the stirring mechanism is achieved, the effect of adjusting the stirring state according to the growth condition of methylobacterium is achieved, the shearing force and the mixing efficiency are balanced, and the problem that thalli are damaged due to the fact that traditional equipment cannot balance the shearing force and the mixing efficiency is solved.

Description

Technical Field

[0001] The invention relates to the technical field of culture matrix mixing, in particular to methylobacterium culture equipment. Background Art

[0002] Methylobacterium is a Gram-negative bacterium widely distributed in nature with unique metabolic characteristics and ecological functions. When cultivating Methylobacterium, low concentrations of rare earth elements (such as Ce³⁺ and La³⁺) can stimulate its metabolism. Adding rare earth element-containing materials to the culture medium and mixing them can optimize Methylobacterium's function. However, traditional impellers are inefficient in mixing media with high viscosities or containing solid particles (such as rare earth minerals). Rare earth particles or bacteria can flocculate and settle. Low rare earth concentrations are ineffective, while high concentrations inhibit the growth of Methylobacterium.

[0003] To this end, a Chinese patent with authorization announcement number CN117282321B discloses a mushroom cultivation culture matrix mixing and stirring production equipment. The equipment disperses the rice particles that are gathered and discharged by dropping them onto a material-distributing plate. The reciprocating swing of the material-distributing plate and the inertial force of the rice particles cause them to slide downward. At the same time, the rice particles are diverted by material-distributing forks distributed in a Pascal's triangle, so that the rice particles are dispersed during the material-distributing process. At the same time, gypsum powder sprayed from multiple directions and different heights is wrapped around the surface of the rice particles and falls into a mixing barrel together. That is, pre-wrapping and mixing are performed during the feeding process before stirring, reducing the pressure of subsequent stirring and mixing, and also improving the uniformity of the mixing of gypsum powder and rice particles.

[0004] To ensure uniform mixing during the cultivation of Methylobacterium, the stirring blades typically rotate at high speeds to achieve uniform mixing. However, if the stirring blades rotate too high, local shear stresses exceeding the tensile strength of the cell wall can cause membrane perforation or lysis. This can damage a significant number of cells during the exponential growth phase, compromising the culture. Summary of the Invention

[0005] In response to the above problems, a methylbacterium cultivation device is provided, which solves the problem that traditional equipment cannot balance shear force and mixing efficiency and thus damages bacteria through a fermentation tank, a stirring mechanism and an auxiliary control mechanism.

[0006] In order to solve the problems of the prior art, the present invention provides a methylbacterium cultivation device, comprising a fermentation tank and a stirring mechanism, the fermentation tank comprising a tank body, a cover body and a rotary driver for driving the stirring mechanism; a main shaft of the stirring mechanism and a stirring rod sleeved on the main shaft, the main shaft being transmission-connected to the driving end of the rotary driver; a stirring blade is rotatably provided on the stirring rod; an auxiliary control mechanism for controlling the rotation of the stirring blade is provided in the tank body; when the stirring blade stops rotating and rotates synchronously with the stirring rod, the stirring mechanism is in a first stirring state; when the stirring blade rotates synchronously with the stirring rod and rotates at the same time, the stirring mechanism is in a second stirring state.

[0007] Preferably, the auxiliary control mechanism includes a transmission component and a limiting component; the transmission component is connected to the stirring blade; the limiting component is used to limit the rotation of the stirring blade; when the rotary drive drives the stirring rod to rotate and the limiting component does not limit the rotation of the stirring blade, the transmission component drives the stirring blade to rotate.

[0008] Preferably, the transmission assembly includes a gear ring and a rotating shaft; the gear ring is arranged in the tank body, and the axis of the gear ring is colinear with the axis of the main shaft; the rotating shaft is rotatably arranged on the stirring rod, and a rotating gear is sleeved on the rotating shaft, and the rotating gear is meshed with the gear ring; the stirring blade is sleeved on the rotating shaft.

[0009] Preferably, the lifting mechanism includes a mounting platform; the mounting platform is disposed within the tank body, and the mounting platform is provided with left-handed and right-handed double-ended staggered threads, and the gear ring is threadedly connected to the mounting platform via a double-ended staggered thread; The stirring rod is slidably sleeved on the main shaft along the main shaft axis; the first bracket is rotatably connected to the gear ring, and the rotating shaft is rotatably connected to the first bracket.

[0010] Preferably, the limiting component includes a raised portion arranged at the top of the stirring blade and a first telescopic block movably arranged on the stirring rod; the stirring rod is equipped with a first linear driver for controlling the extension and retraction of the first telescopic tube in the vertical direction; when the raised portion abuts against the first telescopic block, the rotation of the stirring blade is limited.

[0011] Preferably, a second telescopic block is movably provided on the mounting platform, and a second linear driver for controlling the second telescopic block to extend and retract in a vertical direction is built into the mounting platform.

[0012] Preferably, the mounting platform is arranged in the tank body so as to be lifted and lowered in the vertical direction, and a third linear drive is provided on the cover body, and the third linear drive is used to drive the mounting platform to be lifted and lowered.

[0013] Preferably, an observation window is provided on the tank body.

[0014] Preferably, a second bracket is provided on the tank body, a color identifier is provided on the second bracket, and the second bracket is located at the observation window.

[0015] Preferably, a heat conducting strip is provided inside the tank body, and the heat conducting strip extends from the top end to the bottom end of the tank body.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention realizes a dual stirring state function of the stirring mechanism through a fermentation tank, a stirring mechanism, and an auxiliary control mechanism. In the early stage of fermentation, the auxiliary control mechanism restricts the rotation of the stirring blade, and the stirring mechanism enters the first stirring state. At this time, the rotary driver drives the main shaft and stirring rod to rotate at high speed, rapidly mixing the culture medium. When the bacteria enter the exponential growth phase, the auxiliary control mechanism no longer restricts the rotation of the stirring blade, and the stirring mechanism enters the second stirring state. The rotary driver drives the main shaft and stirring rod to rotate and reduces the speed of the main shaft and stirring rod. The torque provided by the rotary driver is transmitted through the auxiliary control mechanism. Under the action of this torque, the stirring blade rotates while the stirring rod revolves. The stirring mechanism enters the second stirring state. The rotation of the stirring blade provides axial thrust to prevent stratification of the fermentation liquid. The stirring state is adjusted according to the growth of Methylobacterium, maintaining the optimal culture conditions of the culture medium, balancing shear force and mixing efficiency, and solving the problem that traditional equipment cannot balance shear force and mixing efficiency and damage bacteria.

[0017] 2. The present invention realizes the function of controlling the rotation of the stirring blade through the transmission component and the limiting component. The stirring blade is limited to rotate by the limiting component in the early stage of fermentation, and the stirring blade is driven to rotate by the transmission component during the exponential growth period of the bacteria, thereby achieving the effect of dynamically adjusting the stirring mode. In the early stage of fermentation, the operator sends a signal to the rotary driver through the controller, and the rotary driver drives the main shaft and the stirring rod to rotate, and limits the rotation of the stirring blade by the limiting component, and utilizes the stirring blade to increase the effective stirring area of ​​the stirring mechanism. In the early stage of fermentation, the circumferential flow of the fluid is enhanced, the concentration gradient is reduced, the stirring uniformity is improved, and the rare earth particles are quickly dispersed.

[0018] 3. The present invention realizes the function of controlling the stirring rod to rise and fall in a small range in the vertical direction in the first stirring state through the mounting platform, the connecting ring and the connecting rod. The pulsed pressure wave is formed by the lifting and lowering movement of the stirring rod, thereby enhancing the diffusion flux of rare earth elements to the surface of the bacteria and further improving the mixing uniformity of the culture matrix. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a three-dimensional schematic diagram of a Methylobacterium cultivation device of the present invention.

[0020] Figure 2 It is a three-dimensional exploded schematic diagram of a methylbacterium cultivation device of the present invention.

[0021] Figure 3 It is a three-dimensional schematic diagram of a stirring mechanism and an auxiliary control mechanism of a Methylobacterium cultivation device of the present invention from a first perspective.

[0022] Figure 4It is a stereoscopic schematic diagram of a stirring mechanism and an auxiliary control mechanism of a Methylobacterium cultivation device of the present invention from a second viewing angle.

[0023] Figure 5 The present invention Figure 4 A local enlarged schematic diagram of point A in the middle.

[0024] Figure 6 It is a three-dimensional schematic diagram of the coordination of a stirring blade and a transmission component of a methylbacterium cultivation device of the present invention.

[0025] Figure 7 The present invention is a stereoscopic schematic diagram of a stirring mechanism, a connecting ring and a connecting rod of a methylbacterium cultivation device.

[0026] Figure 8 It is a three-dimensional schematic diagram of the cooperation between the mounting platform and the gear ring of a Methylobacterium cultivation device of the present invention.

[0027] Figure 9 It is a three-dimensional schematic diagram of a tank body of a Methylobacterium cultivation device of the present invention.

[0028] Figure 10 The present invention Figure 9 A partial enlarged schematic diagram of point B in the middle.

[0029] The numbers in the figure are: 1. fermentation tank; 11. tank body; 111. observation window; 112. second bracket; 1121. color identifier; 113. heat conductive strip; 12. cover; 121. third linear drive; 13. rotary drive; 2. stirring mechanism; 21. main shaft; 22. stirring rod; 221. stirring blade; 3. auxiliary control mechanism; 31. transmission assembly; 311. gear ring; 312. rotating shaft; 3121. rotating gear; 313. first bracket; 314. pulley; 315. transmission belt; 32. limiting assembly; 321. protrusion; 322. first telescopic block; 4. lifting mechanism; 41. mounting platform; 411. second telescopic block; 42. connecting ring; 43. connecting rod. DETAILED DESCRIPTION

[0030] In order to further understand the features, technical means, specific objectives and functions achieved by the present invention, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0031] Reference Figure 1-Figure 3: A methylbacterium cultivation device includes a fermentation tank 1 and a stirring mechanism 2, the fermentation tank 1 includes a tank body 11, a cover body 12 and a rotary driver 13 for driving the stirring mechanism 2; the stirring mechanism 2 has a main shaft 21 and a stirring rod 22 sleeved on the main shaft 21, and the main shaft 21 is transmission-connected to the driving end of the rotary driver 13; a stirring blade 221 is rotatably provided on the stirring rod 22; an auxiliary control mechanism 3 for controlling the rotation of the stirring blade 221 is provided in the tank body 11; when the stirring blade 221 stops rotating and rotates synchronously with the stirring rod 22, the stirring mechanism 2 is in a first stirring state; when the stirring blade 221 rotates synchronously with the stirring rod 22 and rotates at the same time, the stirring mechanism 2 is in a second stirring state.

[0032] The present invention realizes the dual stirring state function of the stirring mechanism 2 through the fermentation tank 1, the stirring mechanism 2 and the auxiliary control mechanism 3. In the early stage of fermentation, the auxiliary control mechanism 3 restricts the rotation of the stirring blade 221, and the stirring mechanism 2 enters the first stirring state. At this time, the rotary driver 13 drives the main shaft 21 and the stirring rod 22 to rotate at high speed, quickly mixing the culture medium. When the bacteria enter the exponential growth phase, the auxiliary control mechanism 3 no longer restricts the rotation of the stirring blade 221, and the stirring mechanism 2 enters the second stirring state. The rotary driver 13 drives the main shaft 21 and the stirring rod 22 to rotate, and reduces the speed of the main shaft 21 and the stirring rod 22. The torque provided by the rotary driver 13 is transmitted by the auxiliary control mechanism 3. Under the action of the torque, the stirring blade 221 rotates while revolving with the stirring rod 22. The stirring mechanism 2 enters the second stirring state. The rotation of the stirring blade 221 provides axial thrust to prevent stratification of the culture medium. The stirring state is adjusted according to the growth of Methylobacterium, maintaining the optimal culture conditions of the culture medium, balancing shear force and mixing efficiency, and solving the problem that traditional equipment cannot balance shear force and mixing efficiency and damages bacteria. The lid 12 of the fermenter 1 is equipped with a controller for human-machine interaction. The rotary driver 13 is preferably a servo motor and is electrically connected to the controller. During the initial fermentation phase, the operator sends a signal to the rotary driver 13 via the controller, and the auxiliary control mechanism 3 limits the rotation of the stirring blade 221. The stirring mechanism 2 enters the first stirring state. After the rotary driver 13 is activated, it drives the main shaft 21 and stirring rod 22 to rotate at high speed, rapidly mixing the culture medium. When Methylobacterium enters the exponential growth phase, simply reducing the stirring rate to reduce shear forces may not be sufficient to maintain optimal culture conditions. The rapid increase in bacterial density during the exponential phase can easily lead to increased viscosity of the culture medium. Conventional radial stirring can easily form "circulation zones" and "dead zones," causing rare earth particles or bacteria to settle at the bottom. This can lead to insufficient local substrate or rare earth concentrations, inhibiting uniform bacterial growth. To this end, the auxiliary control mechanism 3 transmits the torque provided by the rotary driver 13 to drive the stirring blade 221 to rotate. The axial thrust provided by the rotation of the stirring blade 221 prevents stratification of the culture medium. At this time, the controller reduces the output speed of the rotary driver 13, thereby reducing the shear force of the stirring rod 22 and protecting the bacteria.

[0033] Reference Figure 2 and Figure 3 : The auxiliary control mechanism 3 includes a transmission component 31 and a limiting component 32; the transmission component 31 is connected to the stirring blade 221 for transmission; the limiting component 32 is used to limit the rotation of the stirring blade 221; when the rotary driver 13 drives the stirring rod 22 to rotate and the limiting component 32 does not limit the rotation of the stirring blade 221, the transmission component 31 drives the stirring blade 221 to rotate.

[0034] The present invention realizes the function of controlling the rotation of the stirring blade 221 through the transmission component 31 and the limiting component 32. In the early stage of fermentation, the stirring blade 221 is limited to rotate by the limiting component 32. In the exponential growth period of the bacterial cell, the stirring blade 221 is driven to rotate by the transmission component 31, thereby achieving the effect of dynamically adjusting the stirring mode. In the early stage of fermentation, the operator sends a signal to the rotary driver 13 through the controller. The rotary driver 13 drives the main shaft 21 and the stirring rod 22 to rotate, and limits the rotation of the stirring blade 221 by the limiting component 32. The stirring blade 221 is used to increase the effective stirring area of ​​the stirring mechanism 2. In the early stage of fermentation, the circumferential flow of the fluid is enhanced, the concentration gradient is reduced, the stirring uniformity is improved, and the rare earth particles are quickly dispersed. In the exponential growth period of the bacterial cell, the rotation restriction of the stirring blade 221 by the limiting component 32 is released. At this time, the rotary driver 13 drives the main shaft 21 and the stirring rod 22 to rotate, and the stirring rod 22 drives the stirring blade 221 located thereon to move synchronously. As the stirring blade 221 revolves, the transmission assembly 31 controls the stirring blade 221 to rotate. The axial thrust provided by the stirring blade 221 breaks the local concentration gradient, preventing stratification. At the same time, the axial flow of the culture medium pushes the internal flocculants, preventing sedimentation at the bottom of the tank 11.

[0035] Reference Figure 3 、 Figure 4 and Figure 6 : The transmission assembly 31 includes a gear ring 311 and a rotating shaft 312; the gear ring 311 is arranged in the tank body 11, and the axis of the gear ring 311 is colinear with the axis of the main shaft 21; the rotating shaft 312 is rotatably set on the stirring rod 22, and a rotating gear 3121 is sleeved on the rotating shaft 312, and the rotating gear 3121 is meshed with the gear ring 311; the stirring blade 221 is sleeved on the rotating shaft 312.

[0036] The present invention realizes the function of driving the stirring blade 221 to rotate when the stirring rod 22 rotates through the gear ring 311, the rotating shaft 312 and the rotating gear 3121. Two stirring blades 221 and two rotating shafts 312 are provided on both sides of the stirring rod 22. Each rotating shaft 312 is sleeved with a pulley 314, and a transmission belt 315 is sleeved on the pulley 314. The pulleys 314 on the two rotating shafts 312 on the same side are connected by the transmission belt 315. The rotating gear 3121 is sleeved on the rotating shaft 312 near the gear ring 311, and multiple rotating shafts 312 on the same side are connected by the pulley 314 and the transmission belt 315. When the rotating gear 3121 drives the rotating shaft 312 to rotate, the rotating shaft 312 drives the remaining rotating shafts 312 to rotate synchronously through the pulley 314 and the transmission belt 315. When the rotary driver 13 drives the main shaft 21 and the stirring rod 22 to rotate, the stirring rod 22 drives the stirring blade 221 to move synchronously, so that the stirring blade 221 rotates along the axis of the main shaft 21. The gear ring 311 is arranged in the tank body 11 and does not move with the rotation of the main shaft 21. The rotating gear 3121 sleeved on the rotating shaft 312 is meshed with the gear ring 311. When the rotating gear 3121 rotates around the axis of the main shaft 21, it moves relative to the gear ring 311 and then rotates on its own. The rotating gear 3121 drives the rotating shaft 312 and the stirring blade 221 to rotate, and provides axial thrust through the rotation of the stirring blade 221 to avoid stratification of the culture medium.

[0037] Reference Figure 2-Figure 5 : The lifting mechanism 4 includes a mounting table 41; the mounting table 41 is provided in the tank body 11, the mounting table 41 is provided with left-handed and right-handed double-ended staggered threads, the gear ring 311 is threadedly connected to the mounting table 41 by a double-ended staggered thread; The stirring rod 22 is slidably sleeved on the main shaft 21 along the axis of the main shaft 21 ; the first bracket 313 is rotatably connected to the gear ring 311 , and the rotating shaft 312 is rotatably connected to the first bracket 313 .

[0038] The present invention utilizes a mounting platform 41, a connecting ring 42, and a connecting rod 43 to achieve the function of controlling the vertical movement of the stirring rod 22 within a small range in the first stirring state. The movement of the stirring rod 22 generates a pulsed pressure wave, enhancing the diffusion flux of rare earth elements to the bacterial cell surface and further improving the mixing uniformity of the culture medium. A connecting ring 42 is mounted and rotatably connected to the stirring rod 22; a connecting rod 43 is movably mounted on the stirring rod 22 and connected to the connecting ring 42. The arrangement of the connecting ring 42 and connecting rod 43 guides the movement of the gear ring 311 and the stirring rod 22, ensuring stable movement of the rotating stirring rod 22. Limiting rings are provided at both the upper and lower ends of the mounting platform 41 to limit the range of movement of the gear ring 311. The axis of the connecting ring 42 is colinear with the axis of the main shaft 21. The double-start, staggered threads provided on the mounting platform 41 enable the gear ring 311 to perform reciprocating movement on the mounting platform 41 when it rotates continuously in one direction. When the limiting component 32 limits the rotation of the stirring blade 221, the rotating gear 3121 and the rotating shaft 312 are also unable to rotate. At this time, when the stirring rod 22 and the main shaft 21 rotate under the drive of the rotary driver 13, the stirring rod 22 drives the stirring blade 221, the rotating shaft 312 and the rotating gear 3121 to rotate around the axis of the main shaft 21. Since the rotating gear 3121 cannot rotate on its own, the rotating gear 3121 will push the gear ring 311 to rotate, and the gear ring 311 is threadedly connected to the mounting platform 41, and the mounting platform 41 is provided with a double-headed staggered thread, which is equivalent to the thread symmetrically arranged on the reciprocating screw, so that the gear ring 311 can perform a small range of reciprocating lifting and lowering motion on the mounting platform 41 during the rotation process, further improving the mixing effect on the culture medium.

[0039] Reference Figure 3 and Figure 7 : The limiting component 32 includes a protrusion 321 arranged at the top of the stirring blade 221 and a first telescopic block 322 movably arranged on the stirring rod 22; the stirring rod 22 has a built-in first linear driver for controlling the first telescopic tube to extend and retract in the vertical direction; when the protrusion 321 abuts against the first telescopic block 322, the rotation of the stirring blade 221 is limited.

[0040] The present invention realizes the function of limiting the rotation of the stirring blade 221 through the protrusion 321 and the first telescopic block 322. The first linear drive is preferably a linear motor, and the controller is electrically connected to the first linear drive through a slip ring and a wire. When the rotary drive 13 drives the main shaft 21 and the stirring rod 22 to rotate, the stirring rod 22 drives the rotating shaft 312 and the rotating gear 3121 sleeved on the rotating shaft 312 to rotate around the axis of the main shaft 21. At this time, the rotating gear 3121 has a tendency to rotate. In the early stage of fermentation, the first telescopic block 322 is driven to extend by the first linear drive built into the stirring rod 22. When the protrusion 321 on the stirring blade 221 contacts the first telescopic block 322, the rotation of the stirring blade 221 is limited, and then the gear ring 311 is pushed to rotate by the rotating gear 3121, so that the gear ring 311 performs reciprocating lifting motion.

[0041] Reference Figure 4 and Figure 8 : A second telescopic block 411 is movably provided on the mounting platform 41, and the mounting platform 41 is built with a second linear driver for controlling the second telescopic block 411 to extend and retract in the vertical direction.

[0042] The present invention realizes the function of limiting the lifting and lowering of the gear ring 311 through the second telescopic block 411 and the second linear drive. The second linear drive is preferably a linear motor, and the second linear drive is electrically connected to the controller. When the rotation restriction of the stirring blade 221 is released, the first telescopic tube is controlled to retract by the first linear drive on the stirring rod 22, and the rotation of the stirring blade 221 is no longer restricted by the first telescopic tube. At this time, when the rotating driver 13 drives the main shaft 21 and the stirring rod 22 to rotate, the stirring rod 22 drives the rotating shaft 312 and the rotating gear 3121 to rotate around the axis of the main shaft 21. At this time, the rotating gear 3121 also applies pressure to the gear ring 311, so that the gear ring 311 has a rotation tendency. When the gear ring 311 rotates, it moves up and down along the mounting platform 41, thereby affecting the rotation stability of the stirring rod 22. To this end, a second telescopic block 411 is set on the mounting platform 41, and the second telescopic block 411 is driven to extend by the second linear drive built into the mounting platform 41. After the second telescopic block 411 contacts the gear ring 311, the gear ring 311 is restricted from moving up. The upward movement of the gear ring 311 is then restricted, hindering its rotation. This allows the driving force provided by the rotary actuator 13 to stably drive the stirring rod 22, effectively mixing the culture medium. When the cells enter the exponential growth phase, the restriction on the rotation of the stirring blade 221 by the restriction assembly 32 is released, and the rotation speed of the stirring rod 22 is reduced, entering the second stirring state to prevent high shear forces from damaging the cells.

[0043] Reference Figure 1 and Figure 2: The mounting platform 41 is vertically raised and lowered in the tank body 11, and a third linear drive 121 is provided on the cover body 12. The third linear drive 121 is used to drive the mounting platform 41 to rise and fall.

[0044] The present invention realizes the function of driving the mounting platform 41 to rise and fall through the third linear drive 121, and then drives the rotating shaft 312 and the stirring rod 22 to rise and fall through the mounting platform 41, the gear ring 311 and the first bracket 313. The third linear drive 121 is preferably a linear cylinder, and the third linear drive 121 is electrically connected to the controller. In the process of mixing the culture matrix, it is necessary to ensure the mixing uniformity. To this end, a third linear drive 121 is provided for driving the stirring rod 22 to rise and fall. After starting the rotary drive 13, the rotary drive 13 drives the main shaft 21 and the stirring rod 22 to rotate. In this process, a signal is sent to the third linear drive 121 through the controller, and the third linear drive 121 drives the mounting platform 41 to rise and fall in the vertical direction, and then drives the rotating shaft 312 and the stirring rod 22 to rise and fall through the mounting platform 41, the gear ring 311 and the first bracket 313, adjusts the height of the stirring rod 22, and improves the mixing uniformity of the culture matrix. When the raised portion 321 on the stirring blade 221 contacts the first telescopic block 322, the stirring blade 221 is restricted from rotating, and the gear ring 311 is pushed to rotate by the rotating gear 3121. The gear ring 311 drives the stirring rod 22 to perform reciprocating lifting motion through the first bracket 313. The mounting platform 41 is lifted and lowered in the vertical direction under the drive of the third linear drive 121, so that the stirring rod 22 can also perform a small range of reciprocating lifting and lowering motion on the mounting platform 41 during the process of the mounting platform 41 being lifted and lowered.

[0045] Reference Figure 2 、 Figure 9 and Figure 10 : An observation window 111 is provided on the tank body 11.

[0046] The present invention facilitates the operator to obtain information about the internal conditions of the tank body 11 through the observation window 111, and then adjusts the stirring state of the stirring mechanism 2 according to the state of the culture medium in the tank body 11. In the early stage of fermentation, the auxiliary control mechanism 3 is first used to limit the rotation of the stirring blade 221, entering the first stirring state and rapidly mixing the culture mechanism. The operator then observes the state of the culture medium in the tank body 11 through the observation window 111. When the bacteria enter the exponential growth phase, the rotation restriction of the stirring blade 221 by the limiting component 32 is released, and the rotation speed of the stirring rod 22 is reduced, entering the second stirring state to prevent high shear force from damaging the bacteria.

[0047] Reference Figure 2 、 Figure 9 and Figure 10 : A second bracket 112 is provided on the tank body 11, a color identifier 1121 is provided on the second bracket 112, and the second bracket 112 is located at the observation window 111.

[0048] The present invention realizes the function of identifying the color of the culture substrate through the second bracket 112 and the color identifier 1121. The color identifier 1121 is electrically connected to the controller. Methylobacterium begins to secrete carotenoids during the exponential growth phase, and the color of the culture substrate gradually deepens from light pink. A color identifier 1121 for observing the color of the culture substrate is then provided at the observation window 111. The color of the culture substrate is monitored in real time by the color identifier 1121. When the bacteria are in the exponential growth phase, the color identifier 1121 recognizes that the color of the culture substrate has changed, and a feedback signal is given to the controller. The controller releases the rotation restriction on the stirring blade 221 through the limiting component 32, and the stirring mechanism 2 enters the second stirring state, achieving the effect of automatically switching the stirring state.

[0049] Reference Figure 2 and Figure 9 : A heat conducting strip 113 is provided on the inner side of the tank body 11, and the heat conducting strip 113 extends from the top end to the bottom end of the tank body 11.

[0050] The present invention realizes the function of improving the heat conduction efficiency of the culture medium in the tank body 11 through the heat-conducting strips 113. A plurality of heat-conducting strips 113 are provided, and the plurality of heat-conducting strips 113 are distributed in a circular array along the axis of the main shaft 21. Since the high viscosity of the culture medium will hinder heat exchange. The stirring rod 22 needs to be driven up and down by a linear drive for comprehensive stirring, and there will still be a stirring dead angle in a short period of time. The stirring dead angle of the stirring rod 22 will cause heat accumulation, affecting the activity of the methylobacterium enzyme. For this reason, a heat-conducting strip 113 for transmitting heat is provided in the tank body 11 to avoid the situation where the local temperature difference is too high.

[0051] The above embodiments merely represent one or more embodiments of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art may make various modifications and improvements without departing from the scope of the present invention, and these modifications and improvements fall within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be determined by the appended claims.

Claims

1. A methylbacterium cultivation device, comprising a fermentation tank (1) and a stirring mechanism (2), wherein the fermentation tank (1) comprises a tank body (11), a cover (12) and a rotary driver (13) for driving the stirring mechanism (2); It is characterized in that The stirring mechanism (2) comprises a main shaft (21) and a stirring rod (22) sleeved on the main shaft (21), wherein the main shaft (21) is in driving connection with a driving end of a rotary driver (13); A stirring blade (221) is rotatably provided on the stirring rod (22); An auxiliary control mechanism (3) for controlling the rotation of the stirring blade (221) is provided in the tank body (11); When the stirring blade (221) stops rotating and rotates synchronously with the stirring rod (22), the stirring mechanism (2) is in a first stirring state; When the stirring blade (221) rotates synchronously with the stirring rod (22) and simultaneously rotates on its own, the stirring mechanism (2) is in a second stirring state.

2. A methylbacterium cultivation device according to claim 1, characterized in that: The auxiliary control mechanism (3) includes a transmission component (31) and a limiting component (32); The transmission assembly (31) is in transmission connection with the stirring blade (221); The limiting component (32) is used to limit the rotation of the stirring blade (221); When the rotary driver (13) drives the stirring rod (22) to rotate, and the limiting assembly (32) does not limit the rotation of the stirring blade (221), the transmission assembly (31) drives the stirring blade (221) to rotate.

3. A methylbacterium cultivation device according to claim 2, characterized in that: The transmission assembly (31) includes a gear ring (311) and a rotating shaft (312); The gear ring (311) is disposed in the tank body (11), and the axis of the gear ring (311) is collinear with the axis of the main shaft (21); The rotating shaft (312) is rotatably mounted on the stirring rod (22), a rotating gear (3121) is sleeved on the rotating shaft (312), and the rotating gear (3121) is meshedly connected with the gear ring (311); The stirring blade (221) is sleeved on the rotating shaft (312).

4. A methylbacterium cultivation device according to claim 3, characterized in that: The lifting mechanism (4) includes a mounting platform (41); The mounting platform (41) is provided in the tank body (11), and the mounting platform (41) is provided with left-handed and right-handed double-ended staggered threads, and the gear ring (311) is threadedly connected to the mounting platform (41) via the double-ended staggered threads; The stirring rod (22) is slidably sleeved on the main shaft (21) along the axis of the main shaft (21); The gear ring (311) is rotatably connected to a first bracket (313), and the rotating shaft (312) is rotatably connected to the first bracket (313).

5. A methylbacterium cultivation device according to claim 2, characterized in that: The limiting assembly (32) includes a protrusion (321) provided at the top end of the stirring blade (221) and a first telescopic block (322) movably provided on the stirring rod (22); The stirring rod (22) is built with a first linear actuator for controlling the first telescopic tube to extend and retract in the vertical direction; When the raised portion (321) contacts the first telescopic block (322), the stirring blade (221) is restricted from rotating.

6. A methylbacterium cultivation device according to claim 4, characterized in that: A second telescopic block (411) is movably provided on the mounting platform (41), and a second linear driver for controlling the second telescopic block (411) to telescope in a vertical direction is built into the mounting platform (41).

7. The methylbacterium cultivation device according to claim 4, characterized in that: The mounting platform (41) is arranged in the tank body (11) to be lifted and lowered in a vertical direction, and a third linear drive (121) is provided on the cover body (12). The third linear drive (121) is used to drive the mounting platform (41) to be lifted and lowered.

8. The methylbacterium cultivation device according to claim 1, characterized in that: An observation window (111) is provided on the tank body (11).

9. The methylbacterium cultivation device according to claim 8, characterized in that: A second bracket (112) is provided on the tank body (11), a color identifier (1121) is provided on the second bracket (112), and the second bracket (112) is located at the observation window (111).

10. The methylbacterium cultivation device according to claim 1, characterized in that: A heat conducting strip (113) is provided on the inner side of the tank body (11), and the heat conducting strip (113) extends from the top end to the bottom end of the tank body (11).

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