Method for detecting antibacterial property of worsted fabric

Abstract

The invention discloses a method for detecting the antibacterial performance of a worsted fabric, and belongs to the technical field of cross fusion of textile material science and microbiological detection technology, and the method comprises the following steps: cutting a sample with a certain size and a regular shape from a to-be-detected worsted fabric, marking a plurality of different detection positions on the inner side and the outer side of the sample respectively, the method comprises the following steps: inoculating a test strain into a culture medium, placing a prepared sample in a sterile culture dish subjected to sterilization treatment, eluting each detection position marked on the inner and outer sides of the sample by using sterile normal saline, and calculating the antibacterial rates of different positions on the inner and outer sides of the sample by using a calculation formula, according to the detection method, the detection efficiency is improved, a multi-part result is obtained through a single process, and the antibacterial performance detection results of different positions on the inner side and the outer side of the worsted fabric can be obtained at the same time in the single detection process; the tedious process of single-side detection of the fabric or independent detection of the inner side and the outer side of the fabric in an existing method is avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of cross-integration of textile material science and microbial detection technology, and in particular to a method for detecting the antibacterial properties of worsted fabrics. Background Art

[0002] Worsted fabric is a high-quality textile. It utilizes premium raw materials such as fine wool, high-quality cotton, or specialty fibers, and undergoes a sophisticated spinning process to create extremely fine and uniform yarns. Advanced looms are used during the weaving process to create a dense, defined texture. Worsted fabric boasts a refined appearance, a soft, smooth feel, a lustrous sheen, and excellent drape. It is also exceptionally durable, wrinkle-resistant, and durable, while offering excellent breathability and moisture absorption for comfort. Commonly used in high-end suits, shirts, and formal attire, it showcases the wearer's elegance and refined taste, making it a preferred fabric for those seeking quality and style.

[0003] However, with the continuous improvement of people's requirements for health and hygiene, the antibacterial property of worsted fabrics has become an important quality indicator. At present, the existing methods for testing the antibacterial property of worsted fabrics can usually only test one side of the fabric, or require independent testing of the inside and outside of the fabric. This not only increases the time and manpower costs of testing, but may also affect the comparability of the test results on both sides due to changes in the testing environment and operating conditions. In addition, since it is impossible to obtain the test results at different positions on the inside and outside of the fabric at the same time in a single testing process, it is difficult to comprehensively and accurately evaluate the overall antibacterial property of the fabric. Therefore, it is of great practical significance to develop a method that can simultaneously obtain the antibacterial property test results at different positions on the inside and outside of the worsted fabric in a single testing process. To solve the above problems, we propose a method for testing the antibacterial property of worsted fabrics. Summary of the Invention

[0004] The purpose of the present invention is to solve the problem that the existing methods for testing the antibacterial properties of worsted fabrics can only test one side of the fabric, or require independent testing of the inner and outer sides of the fabric, which not only increases the time and manpower costs of testing, but also may affect the comparability of the test results on both sides due to changes in the testing environment and operating conditions. A method for testing the antibacterial properties of worsted fabrics is proposed.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: A method for detecting the antibacterial properties of worsted fabrics comprises the following steps: Sample preparation: Samples of a certain size and regular shape are precisely cut from the worsted fabric to be tested. The cutting process strictly follows the operating specifications to avoid damaging the fabric fiber structure to ensure the original performance of the sample. Multiple different test positions are marked on the inside and outside of the sample, and the marking positions are selected at the four corners and the center of the fabric. Preparation of bacterial solution: The test bacteria are inoculated into the culture medium and cultured under precisely controlled temperature, humidity and incubation time until the bacteria reach a stable logarithmic growth phase. Subsequently, the cultured bacterial solution is gradient diluted with high-precision sterile saline. Through repeated experiments and verification, the required detection concentration range that can accurately reflect the antibacterial properties of the fabric is determined; Inoculation and culture: Place the prepared sample in a sterile culture dish that has been sterilized, ensuring that the sample is in full contact with the bottom of the dish and that there are no wrinkles. Use a pipette to draw an appropriate and equal amount of bacterial solution and drip it onto the marked detection positions on both sides of the sample in a predetermined order. Place the inoculated sample and the dish in a constant temperature and humidity incubator and culture for a certain period of time under temperature and humidity conditions that simulate the actual use environment. Elution and counting: After the incubation, sterile saline is used to fully elute the marked detection positions on both sides of the sample to ensure that the bacteria are completely eluted from the sample surface into the sterile saline. During the elution process, the operation force and time are strictly controlled to avoid damage to the bacteria. The eluted bacterial solution is counted using the internationally accepted bacterial counting method. The experiment is repeated many times and statistical analysis is performed; Result calculation and evaluation: Based on the number of bacteria at each test location, the antibacterial rate at different locations on the inside and outside of the sample is calculated using a calculation formula. Based on the calculated antibacterial rate, combined with industry standards and actual application needs, the antibacterial performance of worsted fabrics is evaluated and an evaluation report is given.

[0006] As a preferred solution, in the result calculation and evaluation step, the calculation formula introduces the formula for standard deviation correction: The first half of the formula is the conventional method for calculating the antibacterial rate, and the second half introduces the concept of standard deviation. is a coefficient determined according to experimental requirements ( The value can be between 0.1 and 0.3), by averaging the standard deviations of the control group and the experimental group and multiplying by the coefficient as an error correction term.

[0007] As a preferred embodiment, in the sample preparation step, the sample is cut into a square with a side length of 8-12 cm. An electric cloth cutting machine is used for cutting to avoid damage to the fiber structure of the fabric. The detection position is marked by punching, labeling or using a special marking pen, and the detection positions marked on the inside and outside of the sample correspond to each other. A micro puncher is used for punching, and the punching diameter is controlled at 1-3 mm. Special labels that are sterile, non-toxic, and not easy to fall off are used for labeling. When using a special marking pen, the ink of the marking pen needs to be specially treated so that it will not affect bacterial growth, and the marking is clear and durable.

[0008] As a preferred embodiment, in the sample preparation step, when marking the detection positions, 3-5 detection positions are marked on the inside of the sample. These positions are located at the edge, center, and transition area between the edge and center of the fabric, respectively, to fully reflect the antibacterial properties of different parts of the fabric. The number of detection positions marked on the outside of the sample corresponds to the number on the inside, and the deviation between the outer marked position and the inner marked position on the vertical projection of the fabric does not exceed 2 mm, ensuring the correspondence and comparability of the inner and outer detection positions.

[0009] As a preferred embodiment, in the bacterial solution preparation step, the test bacteria are one or more of Escherichia coli and Staphylococcus aureus, the culture medium is one of nutrient agar medium and blood agar medium, the culture temperature is 35-39°C, the culture time is 18-24 hours, and the detection concentration after the bacterial solution is diluted is 1×10 5 -1×10 6 CFU / mL.

[0010] As a preferred solution, in the inoculation and culturing steps, the amount of bacterial liquid added to each detection position is 8-12 μL, the culturing temperature of the constant temperature and humidity incubator is 35-39°C, the relative humidity is 85%-95%, and the culturing time is 18-24 hours. During the culturing process, the temperature, humidity and carbon dioxide concentration environmental parameters in the incubator are monitored in real time to ensure their stability and avoid affecting the growth of bacteria and the accuracy of the test results due to fluctuations in environmental parameters.

[0011] As a preferred embodiment, in the elution and counting steps, a sterile cotton swab is used to gently wipe the detection position for elution. The cotton swab is made of a soft, fiber-free medical cotton swab. The wiping force is controlled within a range that can fully elute the bacteria without causing damage to the sample. The cotton swab is placed in a test tube containing 5-10 mL of sterile saline and fully shaken for 1-2 minutes to ensure that the bacteria can be evenly dispersed in the saline. The bacterial counting method is the plate count method. 0.1-0.2 mL of the diluted bacterial solution is spread on a solid culture medium plate. A professional applicator is used in the coating process to ensure that the bacterial solution is evenly distributed on the surface of the plate. After culturing for 24 hours under appropriate culture conditions, the number of colonies on the plate is counted, and a multi-person review method is used in the statistical process.

[0012] As a preferred option, when evaluating the antibacterial performance of worsted fabrics based on the antibacterial rate, the antibacterial performance level is set as follows: an antibacterial rate of more than 90% is excellent, indicating that the fabric has extremely strong antibacterial ability and can effectively inhibit the growth and reproduction of bacteria; an antibacterial rate of 70%-90% is good, indicating that the fabric has certain antibacterial properties and can reduce the breeding of bacteria to a certain extent; 50%-70% is average, which means that the antibacterial performance of the fabric needs to be improved and further antibacterial treatment measures need to be taken; less than 50% is unqualified, indicating that the antibacterial performance of the fabric is poor and cannot meet the needs of actual application.

[0013] As a preferred embodiment, in the inoculation and culturing steps, the sample is kept flat when placed in a sterile culture dish, and the culture dish is strictly sterilized before use. The sterilization method adopts high-pressure steam sterilization, the sterilization temperature is 121°C, and the sterilization time is 15-20 minutes to ensure that there is no microbial contamination in the culture dish. The material of the culture dish is transparent, high-temperature resistant, and high-pressure resistant glass or plastic material, which is convenient for observing the changes of the sample during the culturing process. In the process of placing the sample in the culture dish and removing it from the culture dish, sterile operation technology is adopted to avoid contamination by external microorganisms.

[0014] As a preferred solution, a strict quality control system is established throughout the entire testing process, the operating procedures, environmental parameters, and experimental data of each step are recorded and monitored in detail, and the testing equipment and instruments are calibrated and maintained regularly.

[0015] Compared with the prior art, the present invention has the following beneficial effects: The detection method of the present application improves detection efficiency and obtains results for multiple locations through a single process. This solution can simultaneously obtain antibacterial performance test results for different locations on the inside and outside of worsted fabrics in a single detection process, avoiding the cumbersome process of testing only one side of the fabric or testing both the inside and outside independently in existing methods, greatly shortening detection time and reducing labor costs. By completing the detection of both the inside and outside of the fabric through a unified operating process, the environmental changes and operational errors caused by multiple operations are reduced, improving detection efficiency while also ensuring detection accuracy.

[0016] During sample preparation, the test positions of the inner and outer marks on the sample correspond to each other, and the deviation between the outer and inner mark positions on the vertical projection of the fabric does not exceed 2mm, ensuring the correspondence and comparability of the inner and outer test positions, so that the test results can more accurately reflect the overall antibacterial performance of the fabric. The antibacterial performance of the fabric is comprehensively evaluated through multi-position marking. Three to five test positions are marked on the inside of the sample. These positions are located at the edge, center, and transition area between the edge and center of the fabric. The number of test positions marked on the outer side of the sample and the corresponding number of test positions on the inside can fully reflect the antibacterial performance of different parts of the fabric, avoiding the evaluation deviation caused by a single test position, and providing more comprehensive data support for accurately evaluating the overall antibacterial performance of the fabric.

[0017] This application introduces standard deviation correction detection to improve the scientificity and standardization of the detection method. In the result calculation and evaluation steps, the calculation formula introduces standard deviation correction. By averaging the standard deviations of the control group and the experimental group and multiplying them by a coefficient as an error correction term, the error factors in the experimental process are taken into account, making the calculation of the antibacterial rate more scientific and reasonable, and improving the scientificity and reliability of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a flow chart of a method for detecting the antibacterial properties of worsted fabrics proposed in the present invention. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] In the description of the present invention, it should be noted that the terms "upper," "lower," "inner," "outer," "top / bottom," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "provided with," "mounted / connected," and "connected" should be understood in a broad sense. For example, "connected" may refer to a fixed connection, a detachable connection, or an integral connection. It may be a mechanical connection or an electrical connection. It may be a direct connection or an indirect connection through an intermediate medium. It may also refer to internal communication between two components. Those skilled in the art will be able to understand the specific meanings of the above terms in the present invention according to specific circumstances.

[0022] Example, see Figure 1 A method for detecting the antibacterial properties of worsted fabrics comprises the following steps: Sample preparation: Samples of a certain size and regular shape are precisely cut from the worsted fabric to be tested. The cutting process strictly follows the operating specifications to avoid damaging the fabric fiber structure to ensure the original performance of the sample. Multiple different test positions are marked on the inside and outside of the sample, and the marking positions are selected at the four corners and the center of the fabric. Preparation of bacterial solution: The test bacteria are inoculated into the culture medium and cultured under precisely controlled temperature, humidity and incubation time until the bacteria reach a stable logarithmic growth phase. Subsequently, the cultured bacterial solution is gradient diluted with high-precision sterile saline. Through repeated experiments and verification, the required detection concentration range that can accurately reflect the antibacterial properties of the fabric is determined; Inoculation and culture: Place the prepared sample in a sterile culture dish that has been sterilized, ensuring that the sample is in full contact with the bottom of the dish and that there are no wrinkles. Use a pipette to draw an appropriate and equal amount of bacterial solution and drip it onto the marked detection positions on both sides of the sample in a predetermined order. Place the inoculated sample and the dish in a constant temperature and humidity incubator and culture for a certain period of time under temperature and humidity conditions that simulate the actual use environment. Elution and counting: After the incubation, sterile saline is used to fully elute the marked detection positions on both sides of the sample to ensure that the bacteria are completely eluted from the sample surface into the sterile saline. During the elution process, the operation force and time are strictly controlled to avoid damage to the bacteria. The eluted bacterial solution is counted using the internationally accepted bacterial counting method. The experiment is repeated many times and statistical analysis is performed; Result calculation and evaluation: Based on the number of bacteria at each test location, the antibacterial rate at different locations on the inside and outside of the sample is calculated using a calculation formula. Based on the calculated antibacterial rate, combined with industry standards and actual application needs, the antibacterial performance of worsted fabrics is evaluated and an evaluation report is given.

[0023] In the result calculation and evaluation steps, the calculation formula introduces the formula for standard deviation correction: The first half of the formula is the conventional method for calculating the antibacterial rate, and the second half introduces the concept of standard deviation. is a coefficient determined according to experimental requirements ( The value can be between 0.1 and 0.3), by averaging the standard deviations of the control group and the experimental group and multiplying by the coefficient as an error correction term.

[0024] In the sample preparation step, the sample size is cut into a square with a side length of 8-12 cm. An electric cloth cutting machine is used for cutting to avoid damage to the fiber structure of the fabric. The detection position is marked by punching, labeling or using a special marking pen, and the detection positions marked on the inside and outside of the sample correspond to each other. A micro puncher is used for punching, and the punching diameter is controlled at 1-3 mm. Special labels that are sterile, non-toxic, and not easy to fall off are used for labeling. When using a special marking pen, the ink of the marking pen needs to be specially treated so that it will not affect bacterial growth, and the marking is clear and durable.

[0025] The operation method for cutting includes the following steps: Clean the fabric: Before cutting, make sure the surface of the worsted fabric is clean and tidy, free of dust, debris, etc. You can use a soft brush to gently brush off the dust on the surface of the fabric, or wipe it with a clean damp cloth (for some fabrics that are not water-resistant, be careful to use a damp cloth).

[0026] Flatten the fabric: Lay the worsted fabric flat on the cutting table and smooth it out by hand or with a special tool (such as an iron) to remove wrinkles and unevenness. For example, for some easily wrinkled worsted silk fabrics, use a low-temperature iron to gently iron them to make the surface smooth and easy to cut.

[0027] Marking Cutting Lines: Use chalk or a marker to accurately mark the cutting lines on the worsted fabric according to the design. Keep the lines clear and smooth, avoiding blurring or breakage. For example, when marking straight lines, use a ruler to ensure they remain straight. When marking curves, use smooth lines consistent with the design.

[0028] Secure the fabric: To prevent the fabric from shifting during the cutting process, use pins or clips to secure it to the cutting table. Be careful not to puncture the fabric or clamp it too tightly, as this can affect the cutting quality. For example, when securing worsted fabric, keep the pins approximately 10-15 cm apart to ensure stability without causing excessive damage.

[0029] Cutting order: Generally, cut the large outlines first, then the smaller details. For example, when cutting a worsted shirt, first cut out the large areas like the body and sleeves, then work on the details like the collar, cuffs, and pockets. This ensures cutting accuracy and efficiency, and avoids affecting larger areas while cutting smaller areas.

[0030] Cutting force and speed: When using electric cutting equipment, control the cutting force and speed according to the equipment's operating instructions. When cutting manually, maintain even force and a moderate speed. For example, when cutting worsted fabrics, do not apply excessive force, as this may damage the fabric by cutting too deeply. Also, do not cut too quickly to ensure the blade accurately follows the marked lines.

[0031] Checking Cutting Quality: After cutting, carefully inspect the fabric to ensure it meets design requirements, including size, shape, and cut smoothness. If any deviations or uneven cuts are found, correct them promptly. For example, for fabrics with minor dimensional deviations, use scissors for minor adjustments. For fabrics with uneven cuts, use sandpaper or a polishing tool to smooth them out.

[0032] Fabric finishing: Sort and arrange the cut worsted fabrics by part and size to facilitate subsequent sewing. Also, be careful to protect the edges of the fabric to avoid secondary damage during the finishing process. For example, wrap the cut fabrics in clean paper or plastic wrap to protect them from dust, moisture, and other contaminants.

[0033] The special treatment that marker inks need to undergo can be achieved through ingredient adjustment and process optimization to ensure that they do not affect bacterial growth and that the markings are clear and long-lasting. The details are as follows: Traditional marker inks often use alcohol as a solvent, but alcohol solvents are toxic and have a strong odor. Water-based marker inks are now being used instead of alcohol. These are inexpensive, safe, environmentally friendly, harmless to the human body, and do not affect bacterial growth. For example, Nakanishi's erasable water-based marker ink uses water as the solvent, a colorant dispersed or dissolved in water, a water-soluble polyvinyl acetal resin as a film-forming agent, liquid latex, and some film-blocking agents to create the erasable ink. This ink is non-toxic and odorless.

[0034] Adding appropriate additives to ink, such as stabilizers and surfactants, can help improve its performance and stability. For example, a black erasable ink formula contains α-cyclodextrin as a stabilizer, sodium dioctyl sulfosuccinate, and a fluorosurfactant (Surflon S141) as a surfactant. These additives make the ink smoother during writing and maintain viscosity over time without adversely affecting bacterial growth.

[0035] During the ink production process, parameters such as temperature, pressure, and reaction time are strictly controlled to ensure the ink's quality and stable performance. For example, when preparing water-based marker ink, the mixing ratio of various ingredients and reaction conditions must be precisely controlled to ensure that the ink's tinting strength, drying speed, and erasability meet the required properties.

[0036] Special treatment processes, such as filtration and sterilization, are used to remove impurities and microorganisms from the ink, preventing them from affecting bacterial growth. For example, during the ink production process, fine filtration equipment is used to remove particulate impurities, and high-temperature sterilization or ultraviolet sterilization are used to remove microorganisms to ensure the purity and safety of the ink.

[0037] During the sample preparation step, when marking the test positions, mark 3-5 test positions on the inside of the sample. These positions are located at the edge, center, and transition area between the edge and center of the fabric, so as to fully reflect the antibacterial properties of different parts of the fabric. The number of test positions marked on the outside of the sample corresponds to the number on the inside, and the deviation between the outer and inner marked positions on the vertical projection of the fabric does not exceed 2mm, ensuring the correspondence and comparability of the inner and outer test positions.

[0038] In the bacterial solution preparation step, the test strain is one or more of Escherichia coli and Staphylococcus aureus, the culture medium is one of nutrient agar medium and blood agar medium, the culture temperature is 35-39°C, the culture time is 18-24 hours, and the detection concentration after the bacterial solution is diluted is 1×10 5 -1×10 6 CFU / mL.

[0039] During the inoculation and cultivation steps, the amount of bacterial liquid added to each detection position is 8-12μL, the cultivation temperature of the constant temperature and humidity incubator is 35-39℃, the relative humidity is 85%-95%, and the cultivation time is 18-24 hours. During the cultivation process, the temperature, humidity and carbon dioxide concentration environmental parameters in the incubator are monitored in real time to ensure their stability and avoid affecting the growth of bacteria and the accuracy of the test results due to fluctuations in environmental parameters.

[0040] In the elution and counting steps, use a sterile cotton swab to gently wipe the test site for elution. The cotton swab should be made of soft, fiber-free medical cotton swabs. The wiping force should be controlled within the range that can fully elute the bacteria without causing damage to the sample. Place the cotton swab in a test tube containing 5-10mL of sterile saline and shake it thoroughly for 1-2 minutes to ensure that the bacteria can be evenly dispersed in the saline. The bacterial counting method is the plate count method. Take 0.1-0.2mL of the diluted bacterial solution and spread it on the solid culture medium plate. The coating process uses a professional applicator to ensure that the bacterial solution is evenly distributed on the plate surface. After culturing for 24 hours under appropriate culture conditions, count the number of colonies on the plate. The statistical process adopts a multi-person review method.

[0041] When evaluating the antibacterial properties of worsted fabrics based on the antibacterial rate, the antibacterial performance levels are set as follows: an antibacterial rate of more than 90% is excellent, indicating that the fabric has extremely strong antibacterial ability and can effectively inhibit the growth and reproduction of bacteria; an antibacterial rate of 70%-90% is good, indicating that the fabric has certain antibacterial properties and can reduce the growth of bacteria to a certain extent; 50%-70% is average, which means that the antibacterial performance of the fabric needs to be improved and further antibacterial treatment measures need to be taken; and a rate below 50% is unqualified, indicating that the antibacterial performance of the fabric is poor and cannot meet the needs of actual applications.

[0042] During the inoculation and culturing steps, the sample is kept flat when placed in a sterile culture dish, and the culture dish is strictly sterilized before use. The sterilization method adopts high-pressure steam sterilization, the sterilization temperature is 121°C, and the sterilization time is 15-20 minutes to ensure that there is no microbial contamination in the culture dish. The material of the culture dish is transparent, high-temperature and high-pressure resistant glass or plastic, which is convenient for observing the changes of the sample during the culturing process. When placing the sample in the culture dish and removing it from the culture dish, sterile operation technology is used to avoid contamination by external microorganisms.

[0043] Throughout the entire testing process, a strict quality control system is established to record and monitor the operating procedures, environmental parameters, and experimental data of each step in detail, and to calibrate and maintain testing equipment and instruments regularly.

[0044] The detection method of the present application improves detection efficiency and obtains results for multiple locations through a single process. This solution can simultaneously obtain antibacterial performance test results for different locations on the inside and outside of worsted fabrics in a single detection process, avoiding the cumbersome process of testing only one side of the fabric or testing both the inside and outside independently in existing methods, greatly shortening detection time and reducing labor costs. By completing the detection of both the inside and outside of the fabric through a unified operating process, the environmental changes and operational errors caused by multiple operations are reduced, improving detection efficiency while also ensuring detection accuracy.

[0045] During sample preparation, the test positions of the inner and outer marks on the sample correspond to each other, and the deviation between the outer and inner mark positions on the vertical projection of the fabric does not exceed 2mm, ensuring the correspondence and comparability of the inner and outer test positions, so that the test results can more accurately reflect the overall antibacterial performance of the fabric. The antibacterial performance of the fabric is comprehensively evaluated through multi-position marking. Three to five test positions are marked on the inside of the sample. These positions are located at the edge, center, and transition area between the edge and center of the fabric. The number of test positions marked on the outer side of the sample and the corresponding number of test positions on the inside can fully reflect the antibacterial performance of different parts of the fabric, avoiding the evaluation deviation caused by a single test position, and providing more comprehensive data support for accurately evaluating the overall antibacterial performance of the fabric.

[0046] This application introduces standard deviation correction detection to improve the scientificity and standardization of the detection method. In the result calculation and evaluation steps, the calculation formula introduces standard deviation correction. By averaging the standard deviations of the control group and the experimental group and multiplying them by a coefficient as an error correction term, the error factors in the experimental process are taken into account, making the calculation of the antibacterial rate more scientific and reasonable, and improving the scientificity and reliability of the test results.

[0047] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A method for detecting the antibacterial properties of worsted fabrics, characterized in that: The following steps are involved: Sample preparation: Cut a regularly shaped sample from the worsted fabric to be tested, and mark multiple different test positions on the inside and outside of the sample. The marking positions should be selected at the four corners and the center of the fabric. Preparation of bacterial solution: The test bacteria are inoculated into the culture medium and cultured under precisely controlled temperature, humidity and incubation time until the bacteria reach a stable logarithmic growth phase. Subsequently, the cultured bacterial solution is gradient diluted with sterile saline. Through repeated experiments and verification, the required detection concentration range that can accurately reflect the antibacterial performance of the fabric is determined; Inoculation and culture: Place the prepared sample in a sterile culture dish that has been sterilized, ensuring that the sample is in full contact with the bottom of the dish and that there are no wrinkles. Use a pipette to draw an appropriate and equal amount of bacterial solution and drip it onto the marked detection positions on both sides of the sample in a predetermined order. Place the inoculated sample and the dish in a constant temperature and humidity incubator. Elution and counting: After the incubation, use sterile saline to fully elute the marked detection positions on both sides of the sample to ensure that the bacteria are completely eluted from the sample surface into the sterile saline. The eluted bacterial solution is counted using the internationally accepted bacterial counting method. Result calculation and evaluation: Based on the number of bacteria at each test location, the antibacterial rate at different locations on the inside and outside of the sample is calculated using a calculation formula. Based on the calculated antibacterial rate, combined with industry standards and actual application needs, the antibacterial performance of worsted fabrics is evaluated and an evaluation report is given.

2. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the result calculation and evaluation step, the calculation formula introduces a formula for standard deviation correction; The first half of the formula is the conventional method for calculating the antibacterial rate, and the second half introduces the concept of standard deviation. is a coefficient determined according to experimental requirements ( The value can be between 0.1 and 0.3), by averaging the standard deviations of the control group and the experimental group and multiplying by the coefficient as an error correction term.

3. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the sample preparation step, the sample is cut into a square size with a side length of 8-12 cm. An electric cloth cutting machine is used for cutting to avoid damage to the fiber structure of the fabric. The detection position is marked by punching, labeling or using a special marking pen, and the detection positions marked on the inside and outside of the sample correspond to each other. A micro puncher is used for punching, and the punching diameter is controlled at 1-3 mm. Special labels that are sterile, non-toxic, and not easy to fall off are used for labeling. When using a special marking pen, the ink of the marking pen needs to be specially treated so that it will not affect bacterial growth, and the marking is clear and durable.

4. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the sample preparation step, when marking the test positions, 3-5 test positions are marked on the inside of the sample. These positions are located at the edge, center, and transition area between the edge and center of the fabric, respectively, to fully reflect the antibacterial properties of different parts of the fabric. The number of test positions marked on the outside of the sample corresponds to the number on the inside, and the deviation between the outer and inner marked positions on the vertical projection of the fabric does not exceed 2 mm, ensuring the correspondence and comparability of the inner and outer test positions.

5. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, characterized in that: In the bacterial solution preparation step, the test bacteria are one or more of Escherichia coli and Staphylococcus aureus, the culture medium is one of nutrient agar medium and blood agar medium, the culture temperature is 35-39°C, the culture time is 18-24 hours, and the detection concentration after the bacterial solution is diluted is 1×10 5 -1×10 6 CFU / mL.

6. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the inoculation and cultivation steps, the amount of bacterial liquid added to each detection position is 8-12 μL, the cultivation temperature of the constant temperature and humidity incubator is 35-39°C, the relative humidity is 85%-95%, and the cultivation time is 18-24 hours. During the cultivation process, the temperature, humidity and carbon dioxide concentration environmental parameters in the incubator are monitored in real time to ensure their stability and avoid affecting the growth of bacteria and the accuracy of the test results due to fluctuations in environmental parameters.

7. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the elution and counting steps, a sterile cotton swab is used to gently wipe the detection position for elution. The cotton swab is made of a soft, fiber-free medical cotton swab. The wiping force is controlled within a range that can fully elute the bacteria without damaging the sample. The cotton swab is placed in a test tube containing 5-10 mL of sterile saline and fully shaken for 1-2 minutes to ensure that the bacteria can be evenly dispersed in the saline. The bacterial counting method is the plate count method. 0.1-0.2 mL of the diluted bacterial solution is spread on a solid culture medium plate. A professional applicator is used in the coating process to ensure that the bacterial solution is evenly distributed on the surface of the plate. The number of colonies on the plate is counted after culturing for 24 hours under appropriate culture conditions. The statistical process adopts a multi-person review method.

8. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: When evaluating the antibacterial properties of worsted fabrics based on the antibacterial rate, the antibacterial performance levels are set as follows: an antibacterial rate of more than 90% is excellent, indicating that the fabric has extremely strong antibacterial ability and can effectively inhibit the growth and reproduction of bacteria; an antibacterial rate of 70%-90% is good, indicating that the fabric has certain antibacterial properties and can reduce the growth of bacteria to a certain extent; 50%-70% is average, which means that the antibacterial performance of the fabric needs to be improved and further antibacterial treatment measures need to be taken; and a rate below 50% is unqualified, indicating that the antibacterial performance of the fabric is poor and cannot meet the needs of actual applications.

9. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: In the inoculation and culturing steps, the sample is kept flat when placed in a sterile culture dish, and the culture dish is strictly sterilized before use. The sterilization method adopts high-pressure steam sterilization, the sterilization temperature is 121°C, and the sterilization time is 15-20 minutes to ensure that there is no microbial contamination in the culture dish. The material of the culture dish is transparent, high-temperature resistant, and high-pressure resistant glass or plastic material, which is convenient for observing the changes of the sample during the culturing process. When placing the sample in the culture dish and removing it from the culture dish, aseptic operation technology is adopted to avoid contamination by external microorganisms.

10. The method for detecting the antibacterial properties of worsted fabrics according to claim 1, wherein: During the entire testing process, a strict quality control system is established to record and monitor the operating procedures, environmental parameters, and experimental data of each step in detail, and to calibrate and maintain the testing equipment and instruments on a regular basis.

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