Traditional Chinese medicinal material experimental sample embedding and fixing device and use method

Abstract

The invention discloses a traditional Chinese medicinal material experimental sample embedding and fixing device and a use method, and belongs to the technical field of test sample preparation devices, the traditional Chinese medicinal material experimental sample embedding and fixing device comprises a thermal insulation shell, an embedding manipulator, an evaporator and a paraffin supply device. Wherein the bottom of the heat preservation shell is provided with a subpackaging vessel, the top of the heat preservation shell is provided with a displacement mechanism, the displacement mechanism is provided with an embedding mechanical arm, the mechanical arm is dragged by the displacement mechanism to move in the heat preservation shell so as to pick up medicinal material seeds, meanwhile, embedding operation on the medicinal material seeds is completed, and a sample subjected to paraffin packaging is spitted out; the embedding manipulator comprises an air pump, a push-pull electromagnet, a clamping jaw and a rubber pipe, and the rubber pipe is arranged in the embedding guide pipe and can be connected with the air pump and the paraffin supply device at the same time. During specific implementation, firstly, seeds are sucked into the rubber tube by the air pump, when the rubber tube is clamped by the clamping jaw, the air pump stops working, and molten paraffin is filled into the rubber tube by the paraffin supply device so as to wrap a sample.

Description

Technical Field

[0001] The invention relates to the technical field of test sample preparation devices, and in particular to a Chinese medicinal material experimental sample embedding and fixing device and a use method. Background Art

[0002] The principle of the embedding seed method is to mix the tissue sample with a polymer and solidify it under pressure and temperature. This process is called embedding because the tissue sample is encased in the polymer. The most commonly used polymer is paraffin wax because it provides sufficient hardness and cutting properties.

[0003] Under experimental conditions, seeds need to be cleaned, soaked and infiltrated before embedding. Finally, when embedding is carried out, the physical properties of the seeds have changed, becoming soft and fragile. During the embedding process, the experimenter needs to manually place the sample in the molten paraffin liquid with tweezers and wait for it to solidify. This process requires the staff to carefully pinch the sample and adjust the direction of the sample.

[0004] Obviously, completing the seed embedding process is quite labor-intensive. When batches of samples need to be embedded, the embedding effect is difficult to guarantee. Summary of the Invention

[0005] To this end, the present invention provides a Chinese medicinal material experimental sample embedding and fixing device and a method of use, so as to solve the problem in the prior art that the embedding effect is poor due to the cumbersome manual seed embedding process.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] According to the first aspect of the present invention.

[0008] The present invention discloses a device for embedding and fixing a sample of a Chinese medicinal material experiment, comprising:

[0009] The heat-insulating shell has a dispensing container at the bottom and a displacement mechanism at the top;

[0010] An embedding manipulator is installed on the displacement mechanism and is pulled by the displacement mechanism to move within the heat-insulating shell. The embedding manipulator includes:

[0011] The air pump is connected to the embedded guide tube through a tee pipe at the bottom;

[0012] A push-pull electromagnet is fixedly mounted on the embedding guide tube and is transmission-connected to a clamping claw mounted on the end of the embedding guide tube. The middle portion of the clamping claw is hinged to the tail end of the embedding guide tube, and the clamping claw is V-shaped.

[0013] The rubber tube is arranged in the embedded guide tube and connected to the tee tube. When the push-pull electromagnet is fully extended, the clamping claw clamps the rubber tube.

[0014] Among them, the side of the three-way pipe is connected to the paraffin supply device installed at the rear of the insulation shell. An evaporator is installed at the bottom of the paraffin supply device. The evaporator is used to absorb the heat in the insulation shell. When the claws clamp the seeds, the air pump sucks the seeds into the rubber tube, and the paraffin supply device supplies molten paraffin to the rubber tube to embed the seed samples.

[0015] Furthermore, a rotary chuck is rotatably provided at the end of the clamping jaw.

[0016] Furthermore, the push-pull electromagnet includes:

[0017] The electromagnetic coil has a top portion fixedly connected to an embedded guide tube, and the embedded guide tube is located inside the electromagnetic coil;

[0018] A return spring, the top of which is connected to the electromagnetic coil, and the bottom of which is connected to the telescopic tube, wherein the telescopic tube is slidably sleeved on the outside of the embedded guide tube;

[0019] The connecting rod has an upper end connected to the telescopic tube and a lower end hinged to a pair of supporting rods, and the pair of supporting rods are hinged to the clamping claws.

[0020] Furthermore, the displacement mechanism includes:

[0021] The first motor has an output end coaxially connected to the screw;

[0022] The second motor has an output end coaxially connected to the spline shaft;

[0023] A sliding component, wherein a transmission belt is installed inside, and a telescopic electromagnet is installed on the transmission belt;

[0024] The spline shaft drives the transmission belt to move, and drives the telescopic electromagnet to move through the transmission belt. The telescopic electromagnet is equipped with a push-pull electromagnet and drives the push-pull electromagnet to move up and down.

[0025] Furthermore, the sliding component includes:

[0026] The frame is equipped with a linear displacement sensor to detect the position of the telescopic electromagnet;

[0027] A first pulley is slidably sleeved on the spline shaft and rotatably arranged at one end of the frame, and a nut is fixedly arranged at the other end of the frame, the nut is sleeved on the screw and is threadably connected to the screw;

[0028] The second drive is rotatably arranged on the frame and is connected to the first pulley through the transmission belt.

[0029] Furthermore, the paraffin supply device includes:

[0030] Heat conductor, with thermal resistance wire and melt cavity inside;

[0031] A stepper motor, the output end of which is coaxially driven by a lead screw, the lead screw being located at the axis of the melt chamber;

[0032] A piston is sleeved on the lead screw and slides along a guide groove provided inside the melt chamber;

[0033] Wherein, a feeding funnel and a discharging port are provided on the top of the heat conductor, and both the feeding funnel and the discharging port are communicated with the melt cavity.

[0034] Furthermore, the outer side of the heat-insulating shell is sealed and inlaid with transparent glass.

[0035] Furthermore, the evaporator includes a heat absorption tube and a stand, the stand is installed at the bottom of the paraffin supply device, a fan is installed on the rear side of the paraffin supply device, and a heat absorption tube is installed on the front side, and the heat absorption tube is externally connected to a compressor.

[0036] Furthermore, the packaging vessel includes a culture dish and a dispenser, and the culture dish and the dispenser are both fixedly arranged at the bottom of the insulation shell.

[0037] According to the second aspect of the present invention.

[0038] The method of use disclosed in the present invention, using the embedding and fixing device as described above, comprises the following steps:

[0039] 1. The gripper uses a rotary chuck to pick up seeds from the culture dish. The rotary chuck is vertically upward under the action of gravity.

[0040] Second, the jaws are released, and the air pump sucks air into the rubber tube, while the jaws continue to open to seal the bottom of the rubber tube;

[0041] 3. The paraffin supply device injects molten paraffin into the rubber tube to wrap the seeds. After solidification, the rubber tube is inflated by an air pump to push the embedded sample out of the rubber tube.

[0042] The present invention has the following advantages:

[0043] The present invention adopts the method of picking up seeds by means of a clamping claw, and straightening the direction of the seed sample by means of a rotary clamping head, and then using the suction force generated by an air pump to suck the sample into a rubber tube, while the clamping claw continues to open to seal the bottom of the rubber tube, and finally injecting molten paraffin into the rubber tube to wrap the seeds, and after solidification, inflating the rubber tube by means of an air pump, thereby ejecting the embedded sample from the rubber tube. Compared with the method of manually embedding seeds in the prior art, the present invention can greatly improve the speed of sample processing, simplify the process of sample preservation, and solve the problem in the prior art that the embedding effect is poor due to the cumbersome process of manually embedding seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the embodiments or the description of the prior art. Obviously, the drawings described below are merely exemplary, and those skilled in the art can derive other implementation drawings based on the provided drawings without inventive effort.

[0045] The structures, proportions, sizes, etc. illustrated in this specification are intended only to complement the contents disclosed herein and to facilitate understanding and reading by persons familiar with the art. They are not intended to limit the conditions under which the present invention may be implemented and therefore have no substantive technical significance. Any structural modifications, changes in proportions, or adjustments in sizes, without affecting the efficacy and objectives of the present invention, shall still fall within the scope of the technical contents disclosed herein.

[0046] Figure 1 A three-dimensional diagram of the sample embedding and fixing device provided by the present invention;

[0047] Figure 2 A three-dimensional diagram of the push-pull electromagnet provided by the present invention;

[0048] Figure 3 A front view of the push-pull electromagnet provided by the present invention;

[0049] Figure 4 The present invention provides Figure 3 Cross-sectional view at AA;

[0050] Figure 5 A three-dimensional perspective view of the paraffin supply device provided by the present invention;

[0051] Figure 6 A diagram showing the internal structure of the paraffin supply device provided by the present invention;

[0052] Figure 7 A diagram showing the internal structure of the sliding component provided by the present invention;

[0053] Figure 8A three-dimensional diagram of the displacement mechanism provided by the present invention;

[0054] Figure 9 A schematic diagram of seed embedding provided by the present invention;

[0055] In the figure: 1 insulation shell; 2 displacement mechanism; 21 first motor; 22 screw; 23 second motor; 24 spline shaft; 25 sliding component; 251 frame; 252 linear displacement sensor; 253 first pulley; 254 second pulley; 255 nut; 26 transmission belt; 27 telescopic electromagnet; 3 embedding manipulator; 31 air pump; 32 tee pipe; 33 push-pull electromagnet; 331 electromagnetic coil; 332 return spring; 333 connecting rod; 334 support rod; 335 telescopic tube; 34 embedding guide tube; 35 clamping claw; 36 rubber tube; 4 evaporator; 41 heat absorbing tube; 42 stand; 5 paraffin supply device; 51 heat conductor; 52 thermal resistance wire; 53 stepping motor; 54 melt chamber; 55 discharge port; 56 feeding funnel; 57 piston; 58 guide groove; 59 screw; 6 filling vessel; 61 culture dish; 62 filling device; 7 rotary chuck. DETAILED DESCRIPTION

[0056] The following describes the implementation of the present invention using specific embodiments. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. Obviously, the embodiments described are only a portion of the present invention, not all of it. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are intended to fall within the scope of protection of the present invention.

[0057] Please refer to Figures 1-9 The present invention will now describe the device for embedding and fixing Chinese medicinal material experimental samples. The device comprises an insulating shell 1, an embedding robot 3, an evaporator 4, and a paraffin supply device 5. The bottom of the insulating shell 1 is provided with a dispensing container 6, and the top is equipped with a displacement mechanism 2. The embedding robot 3 is mounted on the displacement mechanism 2 and is pulled by the displacement mechanism 2 to move within the insulating shell 1 to pick up medicinal material seeds.

[0058] In this embodiment, the embedding robot 3 can not only take the medicinal seeds, but also complete the embedding operation on the medicinal seeds and spit out the paraffin-encapsulated samples. The embedding robot 3 includes an air pump 31, a push-pull electromagnet 33, a clamping claw 35 and a rubber tube 36. The rubber tube 36 is arranged in the embedding guide tube 34 and can be connected to the air pump 31 and the paraffin supply device 5 at the same time. Specifically, Figure 3The bottom of the air pump 31 is connected to the embedding guide tube 34 through a tee pipe 32, and the outlet on the side of the tee pipe 32 is connected to the paraffin supply device 5. A push-pull electromagnet 33 is installed on the embedding guide tube 34. The push-pull electromagnet 33 is transmission-connected to the clamping jaw 35 at the end of the guide tube 34 to drive the clamping jaw 35 to open and close. The middle part of the clamping jaw 35 is hinged to the tail end of the embedding guide tube 34. The embedding guide tube 34 is equipped with a rubber tube 36, and the clamping jaw 35 is V-shaped. Therefore, when the clamping jaw 35 is fully opened, the rubber tube 36 can be clamped by the clamping jaw 35. In specific implementation, the air pump 31 first sucks the seeds into the rubber tube 36. When the rubber tube 36 is clamped by the clamping jaw 35, the air pump 31 stops working, and the paraffin supply device 5 fills the rubber tube 36 with molten paraffin to wrap the sample.

[0059] The rubber tube 36 is connected to the tee 32, the side of which is connected to the paraffin supply device 5 mounted on the rear of the insulation shell 1. The bottom of the paraffin supply device 5 is mounted with an evaporator 4, which absorbs heat from the insulation shell 1, thereby rapidly cooling and solidifying the paraffin. After the jaws 35 grasp the seeds, the air pump 31 draws the seeds into the rubber tube 36, and the paraffin supply device 5 supplies molten paraffin to the rubber tube 36 to embed the seed sample.

[0060] In this embodiment, a rotary chuck 7 is rotatably provided at the end of the jaw 35. When the jaw 35 is closed, the sample is picked up by the rotary chuck 7 contacting the sample. When the jaw 35 is lifted, the rotary chuck 7 is affected by gravity and droops vertically, thereby making the length and direction of the seed vertically upward, so that the air pump 31 can suck the seed into the rubber tube 36, and under the squeezing of the inner wall of the rubber tube 36, the seed sample remains in a vertical state.

[0061] In this embodiment, the push-pull electromagnet 33 comprises an electromagnetic coil 331, a return spring 332, and a connecting rod 333. The electromagnetic coil 331 is a fixed structure, and the top of the electromagnetic coil 331 is fixedly connected to the embedded guide tube 34, which is located within the electromagnetic coil 331. When the electromagnetic coil 331 is energized, it generates a magnetic force that pushes the telescopic tube 335, which is slidably mounted outside the embedded guide tube 34. To ensure that the telescopic tube 335 can be reset when the electromagnetic coil 331 is de-energized, the electromagnetic coil 331 is connected to the top of the return spring 332, and the bottom of the return spring 332 is connected to the telescopic tube 335, thereby utilizing the elastic force to reset the telescopic tube 335. Furthermore, the telescopic tube 335 is connected to the upper end of the connecting rod 333, the lower end of which is hingedly connected to a pair of support rods 334, which are hingedly connected to the clamping jaws 35. Thus, the electromagnetic coil 331 can be used to drive the clamping jaws 35 to open and close.

[0062] In some embodiments, the displacement mechanism 2 includes a first motor 21, a screw 22, a second motor 23, a spline shaft 24 and a sliding component 25. The output end of the first motor 21 is coaxially connected to the screw 22, the output end of the second motor 23 is coaxially connected to the spline shaft 24, and the interior of the sliding component 25 is provided with a transmission belt 26, on which a telescopic electromagnet 27 is installed. The telescopic electromagnet 27 is used to drive the embedding robot 3 to move up and down. Specifically, the spline shaft 23 drives the transmission belt 26 to move, and drives the telescopic electromagnet 27 to move through the transmission belt 26. A push-pull electromagnet 33 is installed on the telescopic electromagnet 27, and drives the push-pull electromagnet 33 to move up and down, thereby allowing the embedding robot 3 to move in the insulation shell 1 and be suitable for transporting the encapsulated and embedded samples to the packaging vessel 6.

[0063] In this embodiment, the sliding component 25 includes a frame 251, a first pulley 253, and a second pulley 254. The second pulley 254 is rotatably mounted on the frame 251 and is in transmission connection with the first pulley 253 via a transmission belt 26. A linear displacement sensor 252 is mounted within the frame 251 for detecting the position of the telescopic electromagnet 27. The first pulley 253 is slidably mounted on the spline shaft 23 and is rotatably mounted at one end of the frame 251. A nut 255 is fixedly mounted at the other end of the frame 251. The nut 255 is mounted on the screw 22 and is in threaded transmission connection with the screw 22. Therefore, when the screw 22 rotates, the frame 251 is displaced. When the spline shaft 24 rotates, the transmission belt 26 is driven via the first pulley 253, thereby displacing the embedding robot 3 and controlling its position.

[0064] In some embodiments, the paraffin supply device 5 includes a heat conductor 51, a stepper motor 53, and a piston 57. A heat resistor 52 and a melting chamber 54 are provided inside the heat conductor 51. The heat resistor 52 is energized to generate heat, melting the paraffin powder in the melting chamber 54. The melting chamber 54 is cylindrical, and a lead screw 59 is provided at the axis of the melting chamber 54. The output end of the stepper motor 53 is coaxially driven with a lead screw 59. A piston 57 is sleeved on the lead screw 59. The piston 57 slides along a guide groove 58 provided inside the melting chamber 54. When the lead screw 59 rotates, the piston 57 can also move along the length direction of the melting chamber 54. A feed hopper 56 and a discharge port 55 are provided on the top of the heat conductor 51. The feed hopper 56 is used to store paraffin powder. Both the feed hopper 56 and the discharge port 55 are connected to the melting chamber 54. When the piston 57 moves, the molten paraffin can be injected into the rubber tube 36 through the discharge port 55.

[0065] In some embodiments, the evaporator 4 includes a heat-absorbing tube 41 and a stand 42. The stand 42 is mounted on the bottom of the paraffin supply device 5. A fan is mounted on the rear side of the paraffin supply device 5, and a heat-absorbing tube 41 is mounted on the front side. The heat-absorbing tube 41 is externally connected to a compressor to absorb heat and reduce the temperature inside the insulation shell 1. In this embodiment, the dispensing container 6 includes a culture dish 61 and a dispenser 62. Both the culture dish 61 and the dispenser 62 are fixedly mounted on the bottom of the insulation shell 1.

[0066] Based on the same inventive concept, the method of use disclosed in the present invention, refer to Figure 9 , including the embedding and fixing device proposed above, comprising the following steps:

[0067] First, the gripper 35 uses the rotary chuck 7 to pick up seeds from the culture dish 61. The rotary chuck 7 is vertically upward under the action of gravity;

[0068] Second, the clamping jaws 35 are loosened, and the air pump 31 sucks air into the rubber tube 36. At the same time, the clamping jaws 35 continue to open, sealing the bottom of the rubber tube 36.

[0069] 3. The paraffin supply device 5 injects molten paraffin into the rubber tube 36 to wrap the seeds. After solidification, the rubber tube 36 is inflated by the air pump 31 to push the embedded sample out of the rubber tube 36.

[0070] Although the present invention has been described in detail above using general descriptions and specific embodiments, it will be apparent to those skilled in the art that modifications and improvements may be made thereto. Therefore, such modifications and improvements, without departing from the spirit of the present invention, are intended to be within the scope of protection claimed herein.

Claims

1. A device for embedding and fixing Chinese medicinal material experimental samples, characterized in that: include: The heat-insulating shell (1) has a dispensing container (6) at the bottom and a displacement mechanism (2) at the top. An embedding manipulator (3) is installed on the displacement mechanism (2) and is pulled by the displacement mechanism (2) to move the manipulator (3) within the heat-insulating shell (1). The embedding manipulator (3) comprises: The bottom of the air pump (31) is connected to the embedded guide tube (34) through a three-way pipe (32); A push-pull electromagnet (33) is fixedly mounted on the embedded guide tube (34) and is in transmission connection with a clamping claw (35) mounted on the end of the embedded guide tube (34). The middle portion of the clamping claw (35) is hinged to the tail end of the embedded guide tube (34), and the clamping claw (35) is V-shaped. The rubber tube (36) is arranged in the embedded guide tube (34) and is connected to the three-way tube (32). When the push-pull electromagnet (33) is fully extended, the clamping claw (35) clamps the rubber tube (36); The side of the three-way pipe (32) is connected to a paraffin supply device (5) installed at the rear of the insulation shell (1), and an evaporator (4) is installed at the bottom of the paraffin supply device (5). The evaporator (4) is used to absorb heat in the insulation shell (1). When the clamping claws (35) clamp the seeds, the air pump (31) sucks the seeds into the rubber tube (36), and the paraffin supply device (5) supplies molten paraffin to the rubber tube (36) to embed the seed sample.

2. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: A rotary chuck (7) is rotatably provided at the end of the clamping jaw (35).

3. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: The push-pull electromagnet (33) comprises: The electromagnetic coil (331) has a top fixedly connected to the embedded guide tube (34), and the embedded guide tube (34) is located inside the electromagnetic coil (331); A return spring (332), the top of which is connected to the electromagnetic coil (331), and the bottom of which is connected to a telescopic tube (335), wherein the telescopic tube (335) is slidably sleeved on the outside of the embedded guide tube (34); The connecting rod (333) has an upper end connected to the telescopic tube (335) and a lower end hinged to a pair of supporting rods (334), and the pair of supporting rods (334) are hinged to the clamping claw (35).

4. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: The displacement mechanism (2) comprises: The output end of the first motor (21) is coaxially connected to the screw (22); The second motor (23) has an output end coaxially connected to the spline shaft (24); A sliding member (25) is provided with a transmission belt (26) therein, and a telescopic electromagnet (27) is installed on the transmission belt (26); The spline shaft (23) drives the transmission belt (26) to move, and drives the telescopic electromagnet (27) to move through the transmission belt (26); a push-pull electromagnet (33) is installed on the telescopic electromagnet (27), and drives the push-pull electromagnet (33) to move up and down.

5. The Chinese medicinal material experimental sample embedding and fixing device according to claim 4, characterized in that: The sliding component (25) comprises: The frame (251) is internally provided with a linear displacement sensor (252) for detecting the position of the telescopic electromagnet (27); A first pulley (253) is slidably sleeved on the spline shaft (23) and rotatably arranged on one end of the frame (251); a nut (255) is fixedly arranged on the other end of the frame (251); the nut (255) is sleeved on the screw rod (22) and is threadedly connected to the screw rod (22); The second pulley (254) is rotatably arranged on the frame (251) and is transmission-connected to the first pulley (253) via the transmission belt (26).

6. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: The paraffin supply device (5) comprises: A heat conductor (51) is provided with a heat resistance wire (52) and a melt cavity (54) therein; A stepping motor (53) is coaxially driven with a lead screw (59) at its output end, and the lead screw (59) is located at the axis of the melt chamber (54); A piston (57) is sleeved on the lead screw (59) and slides along a guide groove (58) provided inside the melt chamber (54); A feeding funnel (56) and a discharging port (55) are provided on the top of the heat conductor (51), and both the feeding funnel (56) and the discharging port (55) are communicated with the melt chamber (54).

7. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: The outer side of the heat-insulating shell (1) is sealed and inlaid with transparent glass.

8. The Chinese medicinal material experimental sample embedding and fixing device according to claim 1, characterized in that: The evaporator (4) comprises a heat absorbing pipe (41) and a stand (42), wherein the stand (42) is mounted on the bottom of the paraffin supply device (5), a fan is mounted on the rear side of the paraffin supply device (5), and a heat absorbing pipe (41) is mounted on the front side, wherein the heat absorbing pipe (41) is externally connected to a compressor.

9. The Chinese medicinal material experimental sample embedding and fixing device according to claim 2, characterized in that: The packaging vessel (6) comprises a culture dish (61) and a dispenser (62), and the culture dish (61) and the dispenser (62) are both fixedly arranged at the bottom of the heat-insulating shell (1).

10. A method of use, comprising embedding the fixation device according to claim 9, characterized in that: The steps include:

1. The clamping jaws (35) use the rotary chuck (7) to pick up the seeds from the culture dish (61), and the rotary chuck (7) is vertically upward under the action of gravity; Second, the clamping jaws (35) are loosened, and the air pump (31) sucks air into the rubber tube (36). At the same time, the clamping jaws (35) continue to open and seal the bottom of the rubber tube (36); 3. The paraffin supply device (5) injects molten paraffin into the rubber tube (36) to wrap the seeds. After solidification, the rubber tube (36) is inflated by the air pump (31), thereby pushing the embedded sample out of the rubber tube (36).

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