Abnormality detection method and abnormality detection device

Abstract

In order to accurately detect an abnormality in an abnormality monitoring target device, a physical path that is turned on by a voltage applied by a voltage source and the voltage source are provided. A first current meter connected in series with a physical path and a voltage source reads a first current value. A step (S103) of calculating a standard deviation of the first current value is executed. When the standard deviation is greater than a first threshold value, which is a predetermined threshold value, the first threshold value is greater than the first threshold value. And outputting a step (S104) in which a discharge is generated in an insulated portion of a path other than the physical path.

Description

Technical Field

[0001] The present invention relates to an abnormality detection method and an abnormality detection device technology. Background Art

[0002] Capillary electrophoresis devices exist that analyze the chemical properties of a sample by electrophoresis in a capillary tube filled with a polymer (electrophoretic separation medium). One example of such a capillary electrophoresis device is one that is configured to detect the current flowing between an electrode in a cathode-side buffer solution and a high-voltage power supply, and the current flowing between an electrode in an anode-side buffer solution and a ground line. Alternatively, another example of a capillary electrophoresis device is one that can interrupt electrophoresis based on fluctuations in the current flowing between an electrode in an anode-side buffer solution and a ground line.

[0003] For example, Patent Document 1 discloses an electrophoresis device and an electrophoresis method that "measures the current flowing in the current path during electrophoresis, detects the state of the separation medium, and interrupts the application of voltage to the current path. Preferably, the presence or absence of bubbles in the separation medium is detected based on the time change of the current value, and the application of voltage to the current path is interrupted when bubbles occur" (see abstract).

[0004] In addition, Patent Document 2 discloses a capillary electrophoresis device characterized in that "the electrophoresis device has a capillary 02 and analyzes a sample by electrophoresis, has a heater assembly 60 for heating the capillary, and the heater assembly 60 includes a heater 62 as a heat source and a conductive component 63 at least partially made of metal, the conductive component 63 being in contact with a ground portion and insulated" (see abstract).

[0005] Prior art literature

[0006] Patent Literature

[0007] Patent Document 1: Japanese Patent Application Laid-Open No. 2003-344356

[0008] Patent Document 2: Japanese Patent Application Laid-Open No. 2020-38233 Summary of the Invention

[0009] Problems to be solved by the invention

[0010] Patent Document 1 describes a method for detecting bubbles in a capillary electrophoresis apparatus based on a change in the current value detected by a second ammeter flowing between an electrode and a ground line in an anode-side buffer solution.

[0011] However, the technology described in Patent Document 1 is a structure that detects the presence or absence of bubbles, etc. based on the time change of the current value detected by the second ammeter. That is, the technology described in Patent Document 1 is configured to detect discharges and poor conduction caused by bubbles, etc. generated in the flow path. However, the technology described in Patent Document 1 requires improvements in the detection of discharges generated outside the flow path. When Patent Document 1 was written, the voltage applied to the flow path of the capillary electrophoresis device was suppressed to a low level within a predetermined range. In addition, the size of each part of the capillary electrophoresis device was larger than the current situation. Therefore, when implementing Patent Document 1, the risk of discharges occurring outside the flow path is extremely small.

[0012] Therefore, the bubble detection method described in patent document 1 fully and effectively functions as a discharge (leakage) countermeasure. However, in recent years, the situation surrounding the capillary electrophoresis device has changed. Specifically, the inventors independently discovered that in the process of increasing the voltage applied to the flow path and the demand for miniaturization of the capillary electrophoresis device, the leakage countermeasure only through the flow path has become insufficient. Therefore, it is necessary to have a countermeasure for increasing the tendency of increasing the voltage applied to the flow path and miniaturization of the electrophoresis device. It is generally believed that the high voltage applied to the flow path and the miniaturization of the capillary electrophoresis device will promote the ease of discharge. If discharge occurs in the capillary electrophoresis device, the possibility of inducing a bad electrophoresis result increases, and therefore it is not preferred.

[0013] Examining discharge countermeasures in capillary electrophoresis devices, we can see that they can be broadly categorized into the following two approaches. One is to improve the capillary electrophoresis device to a structure that prevents or minimizes discharge. The other is to capture any potential discharge. In this case, when discharge occurs, the impact of the discharge on the capillary electrophoresis device is minimized by accurately detecting it and safely stopping the device.

[0014] Patent Document 2 can be considered an example of the former. Patent Document 2 discloses a capillary electrophoresis device that is difficult to generate discharge. However, Patent Document 2 does not describe a structure for detecting discharge when it occurs and stopping the capillary electrophoresis device based on the detection result. This is because the technology described in Patent Document 2 is based on the premise that basically no discharge occurs, or only a very small discharge occurs to an extent that has almost no impact on the safety of the device. Under the condition that the applied voltage to the flow path is suppressed to a low level within a predetermined range and the capillary electrophoresis device is sufficiently large to prevent discharge, it is believed that the structure described in Patent Document 2 can fully cope with discharge.

[0015] However, as mentioned above, in recent years, the situation surrounding the capillary electrophoresis device has changed, and there is a tendency to increase the voltage applied to the flow path and the requirement for the miniaturization of the capillary electrophoresis device. In view of this, it is also necessary to assume that due to the extremely high applied voltage and the proximity between the components accompanying the miniaturization of the capillary electrophoresis device, unpredictable discharges are generated outside the flow path, and thus safety measures need to be taken. Therefore, it is insufficient to simply improve the capillary electrophoresis device itself to a structure that is difficult to fully discharge, and further improvement is needed. Under such circumstances, it is not preferable to rely solely on a structure that does not generate discharge itself or is extremely difficult to generate discharge itself, such as that described in patent document 2. Therefore, it is necessary to implement in some form a structure that accurately detects the discharge when a discharge is generated and safely stops the capillary electrophoresis device.

[0016] The present invention has been made in view of the above background, and an object of the present invention is to accurately detect an abnormality in an abnormality monitoring target device.

[0017] Means for solving problems

[0018] In order to solve the above-mentioned problems, the present invention is characterized in that an abnormality detection device that reads a first current value from a first ammeter connected in series with the first path and the voltage source between a first path conducted by a voltage applied by a voltage source and the voltage source executes: a standard deviation calculation step of calculating the standard deviation of the first current value; and a first judgment step of outputting that a discharge has occurred in a second path that is a path other than the first path when the standard deviation is greater than a first threshold value that is a predetermined threshold value.

[0019] Other solutions will be described appropriately in the embodiments.

[0020] Effects of the Invention

[0021] According to the present invention, an abnormality in an abnormality monitoring target device can be accurately detected. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a conceptual diagram of a general anomaly detection system to which an anomaly detection method is applied.

[0023] Figure 2 This is a functional block diagram showing a configuration example of a processing device.

[0024] Figure 3A This is a graph showing the temporal changes in the applied voltage and the source current value in a state where no discharge occurs in the insulating portion.

[0025] Figure 3B This is a graph showing the temporal changes in the applied voltage and the source current value in a state where discharge occurs in an insulating portion.

[0026] Figure 3C This is a partially enlarged view of a graph showing temporal changes in applied voltage and source current value in a state where discharge occurs in an insulating portion.

[0027] Figure 4 This is a diagram showing a method for obtaining a source current value.

[0028] Figure 5 This is a flowchart showing an example of the processing procedure of the first embodiment.

[0029] Figure 6 This is a flowchart showing an example of the processing procedure of the fourth embodiment.

[0030] Figure 7A FIG. 1 is a diagram showing the configuration of an example of a capillary electrophoresis system used in the seventh embodiment.

[0031] Figure 7B This is an enlarged view of the front end of the hollow electrode.

[0032] Figure 8 This is a diagram showing a voltage control circuit for controlling the voltage of a capillary electrophoresis device.

[0033] Figure 9 This is a flowchart showing an example of a processing procedure from the start to the end of analysis in a capillary electrophoresis apparatus.

[0034] Figure 10 This is a flowchart showing an example of a processing procedure for checking a current value according to the seventh embodiment.

[0035] Figure 11 This is a flowchart showing an example of the processing procedure of the eighth embodiment.

[0036] Figure 12 This is a flowchart showing an example of the processing procedure of the ninth embodiment.

[0037] Figure 13 This is a flowchart showing an example of a processing procedure of the tenth embodiment.

[0038] Figure 14 This is a flowchart showing an example of a processing procedure of the eleventh embodiment.

[0039] Figure 15 This is a flowchart showing an example of the processing procedure of the twelfth embodiment.

[0040] Figure 16 This is a flowchart showing an example of a processing procedure of the thirteenth embodiment.

[0041] Figure 17 This is a diagram showing an example of an abnormality detection screen. DETAILED DESCRIPTION

[0042] Hereinafter, various embodiments of the abnormality detection method of the present invention will be described in detail with reference to the accompanying drawings.

[0043] [First embodiment]

[0044] First, refer to Figures 1 to 5 , an overview of the first embodiment is described.

[0045] (Overview of Abnormality Detection System Z)

[0046] Figure 1 This is a conceptual diagram of a general abnormality detection system Z to which the abnormality detection method of the first embodiment is applied.

[0047] exist Figure 1 , a processing device 1 as an abnormality detection device that detects abnormality in the abnormality monitoring target device 2 is connected to the abnormality monitoring target device 2.

[0048] A physical path 26 as a first path is provided in the abnormality monitoring target device 2. In addition, the abnormality monitoring target device 2 includes a positive terminal 25 to which a positive voltage is applied and a negative terminal 24 to which a negative voltage is applied. One of the positive terminal 25 and the negative terminal 24 is connected to a voltage source 23. The voltage source 23 is a DC voltage source. Figure 1 In the example shown, a voltage source 23 is connected to the positive electrode terminal 25. The voltage source 23 applies a voltage to the positive electrode terminal 25 or the negative electrode terminal 24. Figure 1 In the example shown, a voltage source 23 is connected to the positive terminal 25. Source current is the current observed by a source ammeter 21, serving as a first ammeter, directly connected to the voltage source 23. Furthermore, the current observed by a return ammeter 22, serving as a second ammeter, connected to the voltage source 23 via a physical path 26 conducted by the voltage applied by the voltage source 23 is referred to as return current. Furthermore, the voltage source 23 is grounded, and the side opposite to the side connected to the voltage source 23 (the return ammeter 22 side) is also grounded.

[0049] Furthermore, the locations where processing device 1 detects abnormalities include physical path 26 and a second path, namely insulating portion 27, which is a path other than physical path 26. In this embodiment, physical path 26 is the portion energized by the current flowing due to the voltage applied by voltage source 23. Furthermore, insulating portion 27 is the portion other than physical path 26, specifically the portion where discharge, described later, occurs. Physical path 26 is formed between positive electrode terminal 25 and negative electrode terminal 24. Insulating portion 27 is the portion other than physical path 26 and is potentially affected by the applied voltage.

[0050] like Figure 1 As shown, the source ammeter 21 is connected in series with the physical path 26 and the voltage source 23 between them. In contrast, the return ammeter 22 is connected in series with the physical path 26 and the voltage source 23 on the opposite side of the physical path 26 from the voltage source 23.

[0051] The voltage source 23 and the return current meter 22 are grounded in order to make the potentials of the voltage source 23 and the return current meter 22 equal.

[0052] In addition, in the first to third embodiments, the return ammeter 22 can be omitted.

[0053] (Processing device 1)

[0054] Figure 2 1 is a functional block diagram showing a configuration example of the processing device 1. Figure 1 .

[0055] The processing device 1 is a PC or the like and includes a memory 11 composed of RAM or the like, a computing device 12 composed of a CPU, a GPU or the like, and a storage device 13 composed of a HDD, a SSD or the like. Furthermore, the processing device 1 includes an input device 14 such as a keyboard and a mouse, a display, an output device 15 as an output unit, and a communication device 16 for transmitting and receiving information to and from the abnormality monitoring target device 2.

[0056] The program stored in the storage device 13 is loaded into the memory 11 and executed by the arithmetic device 12. Thus, the processing unit 110 and the current value acquisition unit 111, calculation unit 112, determination processing unit 113, control processing unit 114, and output processing unit 115 constituting the processing unit 110 are embodied.

[0057] The current value acquisition unit 111 acquires a source current value as a first current value and a return current value as a second current value from the source ammeter 21 and the return ammeter 22 via the communication device 16 .

[0058] The calculation unit 112 calculates the standard deviation of the source current value and the variation value of the return current value.

[0059] The determination processing unit 113 determines whether an abnormality has occurred in the insulating portion 27 or the physical path 26 based on the standard deviation of the source current value and the fluctuation value of the return current value.

[0060] The control processing unit 114 performs operations such as stopping the abnormality-monitoring target device 2 based on the determination result of the determination processing unit 113 .

[0061] The output processing unit 115 outputs an error, an alarm, or the like to the output device 15 based on the determination result of the determination processing unit 113 .

[0062] The control processing unit 114 is used in the fifth, eighth, and twelfth embodiments, and the output processing unit 115 is used in the second, third, sixth, ninth, eleventh, and thirteenth embodiments.

[0063] The inventors have independently discovered that the abnormality that causes the current fluctuation is not limited to the physical path 26, but also occurs in the form of discharge in the insulating portion 27. Figures 3A to 3C A method of observing the discharge generated in the insulating portion 27 will be described.

[0064] (Time variation of source current)

[0065] Figure 3A 1 and 2 are graphs showing temporal changes in applied voltage and source current value in a state where no discharge occurs in insulating portion 27 . Figure 3B 1 and 2 are graphs showing temporal changes in applied voltage and source current value in a state where discharge occurs in insulating portion 27 . Figure 3C It will Figure 3B The portion indicated by symbol X is an enlarged view.

[0066] exist Figures 3A to 3C In the graph, the first vertical axis represents the source current value (unit: μA), the second vertical axis represents the applied voltage value (voltage) (unit: kV), and the horizontal axis represents the elapsed time of the applied voltage (time) (unit: 10 2 msec). In addition, Figure 3A to Figure 3C In the figure, the solid line represents the current drawn, and the dotted line represents the applied voltage. Figures 3A to 3C In the example, the applied voltage is applied in steps, but this is because the voltage is applied in steps for the purpose of experiment. In practice, the applied voltage is applied in a single step. Figures 3A to 3C In the circuit, the applied voltage is applied in steps, so the source current also changes in steps.

[0067] right Figure 3A and Figure 3B By comparing the chart shown in the figure, we can see the following. Figure 3A , when the insulation part 27 does not generate discharge, the pulling current value shows a flat curve. It can be seen that the pulling current value is stable. This is because there is no current leaking to the outside (insulation part 27), and the pulling current value is stable. In contrast, Figure 3B as well as Figure 3CAs shown, when discharge occurs in the insulating portion 27, the resulting curve shows a slight fluctuation in the source current value. In other words, the source current value is erratic. This is because discharge occurs outside the physical path 26 (i.e., the insulating portion 27), causing the source current value to leak, resulting in an unstable source current value.

[0068] In this embodiment, it is characterized by using Figures 3A to 3C The characteristic of the pull current value as shown in FIG. 1 is used to detect the discharge generated in the insulating portion 27 based on the behavior of the pull current value. Figure 4 This will be described later. Figures 3A to 3C It can be seen that immediately after the applied voltage rises, the pull current value also rises approximately vertically. This is a common phenomenon caused by Ohm's law, because when the resistance value is the same, as the applied voltage rises, the pull current also rises. Therefore, the rising change in the pull current value is not accompanied by abnormal discharge. Therefore, the inventors independently discovered that one of the issues is to distinguish between the change in the pull current value accompanied by abnormal discharge in the insulating portion 27 and other changes in the pull current value. Therefore, in this embodiment, as Figure 3C As shown, the source current value during a predetermined period (period T11) immediately after the applied voltage undergoes a substantial, substantially vertical change is excluded from the basis for discharge detection. Furthermore, one of the characteristics of the abnormality detection method of this embodiment is that discharge detection is performed based on source current values ​​other than the source current value during a period other than the excluded period (period T12). Changes in source current values ​​immediately after a voltage change may be due to changes in the voltage.

[0069] The voltage change is performed by the operator's instruction or by the control computer 400 (see Figure 7A ) instructions. However, the processing device 1 can detect the timing of the voltage change by receiving instructions from the control computer 400. However, as described later, due to fluctuations in the source current value caused by discharge in the insulating portion 27, it is impossible to distinguish whether the fluctuation in the measured source current value is accompanied by a voltage change. Therefore, in this embodiment, the source current value immediately after the voltage change is excluded from the discharge determination.

[0070] Figure 4 This is a diagram showing a method for obtaining a source current value.

[0071] In this embodiment, by using the standard deviation of the source current value, the slight fluctuation of the source current value related to the discharge of the insulating portion 27 is distinguished from the fluctuation according to Ohm's law. The fluctuation according to Ohm's law is based on the control computer 400 (see Figure 7A) changes in voltage. For example, as shown in FIG3 , the pull current value is measured every 100msec (measurement time "t0" to "t10"), so that the pull current value is sampled at 10 points in 1sec. That is, during the sampling period of 1sec, the pull current value is measured (sampled) with a sampling cycle of 100msec. Then, the processing device 1 calculates the standard deviation based on the 10 pull current values ​​obtained during the measured 1msec period. When discharge occurs in the insulating portion 27, the pull current value deviates, so the standard deviation of the pull current value becomes larger than when there is no discharge in the insulating portion 27. In addition, the sampling period of the pull current value is not limited to 1sec, and the sampling cycle of the pull current value is not limited to 100msec. In addition. As shown in FIG3 , the number of sampling times is not limited to 10 times.

[0072] Therefore, in this embodiment, a first threshold is set as a certain threshold value, and when the standard deviation of the source current value exceeds the set first threshold value, it is determined that the source current value is abnormal. The first threshold value is determined by the user taking into account the parameters that affect the source current value.

[0073] (flow chart)

[0074] Next, refer to Figure 5 The abnormality detection method shown in the first embodiment is described with reference to the flowchart of FIG.

[0075] Figure 5 This is a flowchart showing an example of the processing procedure of the first embodiment. Figure 1 .

[0076] Figure 5 The flowchart shown is a process performed every time the source current value is read by the source current meter 21 .

[0077] The current value acquisition unit 111 reads the source current value from the source current meter 21 (S101). The source current value is read by Figure 4 The sampling method shown is used. Figure 4 The source current value is read at the timing of time t0 to t10 shown in FIG. Step S101 is a source current value reading step in which the current value acquisition unit 111 reads the source current value at a predetermined sampling cycle (at each time interval).

[0078] Then, the calculation unit 112 determines whether the set number of times (set number of times: according to Figure 4 The example shown is 10 times) of the pull current value (S102).

[0079] When the source current value has not been read the set number of times ( S102 -> No), the processing device 1 returns the process to step S101 .

[0080] If the source current values ​​have been read the set number of times (S102 → Yes), the calculation unit 112 calculates the standard deviation of the source current values ​​using the read source current values ​​(S103). Step S103 is a standard deviation calculation step. Specifically, in step S103, the calculation unit 112 calculates the standard deviation based on the source current values ​​read at predetermined time intervals.

[0081] Then, the determination processing unit 113 determines whether the standard deviation calculated in step S103 is larger than a first threshold value ( S104 : first determination step).

[0082] When the value of the standard deviation is equal to or smaller than the first threshold value ( S104 →No), the determination processing unit 113 determines that no discharge has occurred in the insulating portion 27 (no discharge) ( S105 ).

[0083] When the value of the standard deviation exceeds the first threshold value (S104→Yes), the determination processing unit 113 determines whether the current state is immediately after the applied voltage has been changed (S106). The determination of step S106 is performed by the determination processing unit 113 determining whether a predetermined time has passed since the applied voltage was changed. As described above, in the case of immediately after the applied voltage has been changed, the pull current value also changes as the applied voltage changes according to Ohm's law. Therefore, when the determination result is "Yes" in step S106, the determination processing unit 113 determines that the change in the pull current value is not associated with discharge in the insulating portion 27, and the determination is retained (S107: exclusion step). In this way, after the voltage applied to the voltage source 23 is changed, an exclusion step is performed to set the pull current value read within a predetermined period as outside the determination object of discharge.

[0084] The extent to which the source current value is excluded from the change in applied voltage can be set to a different value depending on the status of the abnormality monitoring target device 2. Furthermore, the first threshold used in step S104 is a predetermined threshold that can be set by the operator, such as σ or 2σ (σ is the standard deviation).

[0085] On the other hand, in step S106, if the read source current value is not immediately after the applied voltage is changed (S106 → No), the determination processing unit 113 determines that a discharge has occurred in the insulating portion 27 (S108). In this case, as described later, the control processing unit 114 may stop the abnormality monitoring target device 2, or the output processing unit 115 may output an error or an alarm to the output device 15.

[0086] As described above, in the first embodiment, even if the standard deviation of the source current value is greater than the first predetermined threshold, processing device 1 excludes that source current value from the basis for abnormality determination immediately after the applied voltage is changed. Furthermore, if the standard deviation of source current values ​​sampled other than the excluded source current value is less than the first threshold, processing device 1 determines that there is no discharge. This allows accurate detection of discharge in physical path 26. Furthermore, this allows for the selective and accurate extraction of fluctuations in source current values ​​associated with discharge in insulating portion 27.

[0087] Furthermore, in the first embodiment, a discharge is determined to have occurred when the standard deviation of the source current value is greater than a first threshold value. This allows the abnormality monitoring target device 2 to be safely stopped as in the second embodiment described later, or allows the operator of the abnormality monitoring target device 2 to be accurately informed of the abnormality as in the third embodiment described later.

[0088] Furthermore, the processing of step S104 and step S106 may be interchanged.

[0089] [Second embodiment]

[0090] The first embodiment described above describes an abnormality detection method for confirming whether or not discharge has occurred in the insulating portion 27. In contrast, in the second embodiment, the output processing unit 115 outputs an error or the like when discharge has occurred in the insulating portion 27.

[0091] Although not shown in the figure, in the first embodiment, the determination processing unit 113 Figure 6 After it is determined that "discharge occurs" in step S108, an error is output and the abnormal monitoring object device 2 is forcibly stopped. In this respect, the second embodiment is different from the first embodiment, but in other respects, the same processing as the first embodiment is performed. In the second embodiment, when it is determined that a discharge has occurred in the insulating portion 27, the abnormal monitoring object device 2 is forcibly stopped. As a result, the operator will not operate the abnormal monitoring object device 2 in a state where the insulating portion 27 is discharged, thereby preventing damage to the components of the abnormal monitoring object device 2. In addition, the processing performed in the second embodiment is the same as that described later. Figure 12 The same processing as shown.

[0092] [Third embodiment]

[0093] In the first embodiment described above, an abnormality detection method for confirming whether discharge occurs in the insulating portion 27 is described. In the third embodiment, an alarm is output as a specific example of an error output. Although not shown in the figure, the output processing unit 115 in the first embodiment Figure 5In step S108, the third embodiment is different from the first embodiment in that it determines that there is discharge, but the other aspects are the same as the first embodiment. In addition, the processing of the third embodiment is to output the alarm. Figure 12 Step S408B is replaced by the process of outputting an alarm. The output of the alarm may be sounding an alarm by a buzzer (not shown) or displaying the alarm on the output device 15.

[0094] According to the third embodiment, as a specific example of the error output, the output processing unit 115 outputs an alarm so that the operator can be aware of the situation in which discharge occurs in the insulating portion 27 of the abnormality-monitoring target device 2 .

[0095] [Fourth embodiment]

[0096] Figure 6 This is a flowchart showing an example of the processing procedure of the fourth embodiment. Figure 1 .

[0097] The first embodiment described above describes an abnormality detection system Z that can detect discharge generated in the insulating portion 27 by measuring the source current value. In contrast, the fourth embodiment includes steps S201 to S204 as a return current value abnormality detection process.

[0098] The source current is largely unaffected by the state of the physical path 26. Therefore, the source current exhibits a current value derived almost directly from the applied voltage and Ohm's law. In contrast, the return current is significantly affected by the state of the physical path 26. Specifically, if there is an abnormality in the physical path 26 that causes discharge, poor conduction, or the like, the return current is affected by the abnormality. Leveraging this property, the fourth embodiment detects abnormalities in the physical path 26 by observing the return current.

[0099] Specifically, first, the return current value is read by the return current meter 22 ( S201 : second current value reading step).

[0100] Next, the calculation unit 112 calculates the change value of the return current value (S202). Specifically, the determination processing unit 113 calculates the difference between the return current value read last time and the return current value read this time.

[0101] Then, the determination processing unit 113 determines whether the change value of the return current value is larger than the second threshold value ( S203 : second determination step).

[0102] If the change in the return current value is greater than the second threshold value (S203→Yes), the determination processing unit 113 determines that an abnormality has occurred in the physical path 26 (S204: second determination step). Figure 7A In the case of the capillary electrophoresis device 300 shown, the abnormality refers to discharge, conduction failure, etc. generated in the capillary 312 due to bubbles generated in the capillary 312 .

[0103] If the change value of the current value returned in step S203 is less than the second threshold value (S203→No), the current value is read by the current meter 21 (S101). The processing after step S101 is the same as Figure 5 The same treatment is applied.

[0104] The fourth embodiment differs from the first embodiment in that it includes an abnormality detection process based on the return current value, namely steps S201 to S204, but otherwise remains the same as the first embodiment. By adding abnormality detection based on the return current value, steps S201 to S204 enable the fourth embodiment to detect an abnormality in the physical path 26. Furthermore, by displaying abnormality detection based on the return current value and discharge detection based on the source current value separately, the operator can easily determine where the abnormality has occurred.

[0105] [Fifth embodiment]

[0106] In the fourth embodiment described above, the method for confirming Figure 1 In contrast, in the fifth embodiment, when discharge occurs in the insulating portion 27, the control processing unit 114 (see Figure 2 ) is used to forcibly stop the abnormal monitoring target device 2. Although not shown in the figure, it is added to the fourth embodiment. Figure 6 The fifth embodiment is different from the fourth embodiment in this respect, but is the same as the fourth embodiment in other respects. In addition, the processing of the fifth embodiment is the same as that of the fourth embodiment described later. Figure 15 Same treatment.

[0107] According to the fifth embodiment, in the determination processing unit 113 (refer to Figure 2 ) determines that a discharge has occurred in the insulating portion 27, the control processing unit 114 forcibly stops the abnormality monitoring target device 2. This allows the processing device 1 to detect the discharge in the insulating portion 27 and safely stop the abnormality monitoring target device 2. Furthermore, since the operator will not operate the abnormality monitoring target device 2 while a discharge has occurred in the insulating portion 27, damage to the components of the abnormality monitoring target device 2 can be prevented.

[0108] [Sixth embodiment]

[0109] In the fourth embodiment described above, an abnormality detection method for confirming whether an abnormality has occurred in the physical path 26 of the abnormality monitoring target device 2 has been described. In contrast, in the sixth embodiment, when a discharge is detected in the insulating portion 27, an output processing unit 115 (see Figure 2 ) Output error processing. Although not shown, the determination processing unit 113 is added in the fourth embodiment. Figure 6 The output processing unit 115 outputs an error when the judgment in step S106 is "No". The sixth embodiment is different from the fourth embodiment in this respect, but is the same as the fourth embodiment in other respects. According to the sixth embodiment, when a discharge is detected in the insulating portion 27, the output processing unit 115 outputs an error. Thus, the operator can notice that a discharge has occurred in the insulating portion 27 of the abnormality monitoring target device 2. In addition, the processing performed in the sixth embodiment is the same as that described later. Figure 16 The same processing as shown.

[0110] [Seventh embodiment]

[0111] Next, refer to Figures 7A to 9 In the seventh embodiment, a case where the abnormality detection method described in the first embodiment is installed in a capillary electrophoresis device 300 will be described.

[0112] In the following embodiments, Figure 7A In the capillary electrophoresis device 300 shown in FIG. 1 , an insulating portion 27 (see FIG. 1 ) outside the flow path as a sample is used for detecting the sample. Figure 1 ) discharge, using a source current. Processing device 1 then calculates a standard deviation using the source current value. Next, processing device 1 determines whether discharge has occurred in insulating portion 27 based on whether the calculated standard deviation exceeds a threshold. Processing device 1 performing this determination is connected to capillary electrophoresis device 300, thereby detecting discharge generated in capillary electrophoresis device 300.

[0113] (Capillary electrophoresis system 3)

[0114] Figure 7A : is a diagram showing an example of a capillary electrophoresis system 3 used in the seventh embodiment. Figure 7B yes Figure 7A The enlarged view of the portion indicated by symbol Y is an enlarged view of the front end portion of the hollow electrode 313 .

[0115] The capillary electrophoresis system 3 is composed of a capillary electrophoresis device 300 and a control computer 400 .

[0116] The capillary electrophoresis device 300 includes a detection unit 301 for optically detecting a sample and an oven (constant temperature bath) 351 for maintaining the temperature of the capillary 312. In addition, the capillary electrophoresis device 300 includes a cathode end 312A (see Figure 7B ) An automatic sampler 330 for transporting various containers. Furthermore, the capillary electrophoresis apparatus 300 includes a high-voltage power supply 23A for applying a DC high voltage to the capillary 312.

[0117] (Anomaly Detection System)

[0118] Furthermore, the capillary electrophoresis device 300 includes a source ammeter 21 for detecting the current generated by the voltage applied by the high-voltage power supply 23A. In addition, the capillary electrophoresis device 300 includes a return ammeter 22 for detecting the current flowing through the anode electrode 342A. The high-voltage power supply 23A, the source ammeter 21, and the return ammeter 22 originally use the power supply provided by the capillary electrophoresis device 300. In addition, the high-voltage power supply 23A is equivalent to Figure 1 voltage source 23.

[0119] (Capillary array 311)

[0120] The capillary electrophoresis device 300 includes a capillary array 311 composed of one or more capillaries 312. Furthermore, the capillary electrophoresis device 300 includes a pump mechanism 320 or a polymer transport unit for injecting a highly viscous polymer solution (hereinafter referred to as polymer) serving as an electrophoretic medium into the capillary array 311. Furthermore, the capillary electrophoresis device 300 includes a loading head 331 and a capillary head 321.

[0121] As described above, the capillary array 311 includes one or more capillaries 312. Figure 7A In the example shown, the capillary array 311 is composed of eight capillaries 312. The capillary array 311 is a replaceable component.

[0122] When changing the measurement method, that is, when changing the sample, the operator replaces the capillary array 311 and adjusts its length. Furthermore, if damage or quality degradation is observed, the operator replaces the capillary array 311 with a new one. The capillary 312 is made of a glass tube with an inner diameter of tens to hundreds of microns and an outer diameter of several hundred microns. To enhance strength, the surface of the capillary 312 is coated with polyimide. However, the polyimide coating is removed from the detection unit 301, which is irradiated with laser light, to prevent leakage of internal light.

[0123] The interior of the capillary 312 is filled with a polymer, which is a separation medium for imparting a difference in migration velocity during electrophoresis. Polymers have both fluidity and non-fluidity.

[0124] In the loading head 331, a metal hollow electrode 313 (see Figure 7B ).like Figure 7B As shown, the front end of the capillary 312 protrudes from the hollow electrode 313 by about 0.5 mm.

[0125] All hollow electrodes 313 are electrically connected to a high-voltage power supply 23A mounted on the capillary electrophoresis apparatus 300. The hollow electrodes 313 function as cathode electrodes when voltage application is required, such as during electrophoresis or sample introduction.

[0126] The loading head 331 is fixed to the oven 351. The ends (anode ends) of the capillaries 312 located opposite the cathode ends 312A (see FIG7 ) of the capillaries 312 are bundled together by the capillary head 321. Furthermore, the capillaries 312 can be attached and detached from the capillary head 321 in a bundle with a pressure-resistant seal.

[0127] (Pump mechanism 320)

[0128] The pump mechanism 320 includes a pump 322 having a plunger and a block 323 having a flow path therein.

[0129] The inner diameter of the flow path provided inside the block 323 is 0.5 to 2 mm, which is several to dozens of times larger than the inner diameter of the capillary 312. This is to avoid voltage loss during electrophoresis. A pump 322, a capillary head 321, a first tube 343a, and a second tube 343b are connected to the block 323. The pump 322, the capillary head 321, the first tube 343a, and the second tube 343b are interconnected via the flow path provided inside the block 323. Furthermore, the first tube 343a connects the block 323 to the polymer contained in the polymer bottle 341.

[0130] Pump 322 draws polymer from polymer bottle 341, which stores the polymer, via first tube 343a. Pump 322 also draws buffer solution from anode buffer container 342 via second tube 343b. Anode electrode 342A is immersed in the buffer solution in anode buffer container 342. Polymer bottle 341 stores a sufficient volume of polymer for continuous operation. A vent valve (not shown) is provided in polymer bottle 341 to prevent negative pressure inside polymer bottle 341 even when polymer is drawn from polymer bottle 341. Alternatively, a sufficient gap may be provided in polymer bottle 341 at the insertion port of first tube 343a.

[0131] A check valve 344 is provided in the first pipe 343a. In addition, the second pipe 343b connects the block 323 with the buffer solution contained in the anode buffer container 342. An electric buffer valve 345 is provided in the second pipe 343b. Figure 7A Although not explicitly shown, polymer bottle 341 is positioned lower than anode buffer container 342. This is to utilize the pressure generated by the height difference to prevent backflow of polymer from polymer bottle 341 to anode buffer container 342. Conversely, backflow of polymer or buffer solution from anode buffer container 342 to polymer bottle 341 is prevented by check valve 344.

[0132] When injecting polymer into capillaries 312 of capillary array 311, buffer valve 345 is closed. This seals the flow path between capillary array 311 and anode buffer container 342. Pump 322 then drives polymer stored in polymer bottle 341 into capillary 312. During electrophoresis, buffer valve 345 is opened, connecting the flow path between capillary array 311 and anode buffer container 342.

[0133] (Optical Inspection System)

[0134] The optical detection system is composed of a light source 302 for illuminating the detection portion 301 and an optical detector 303 for detecting luminescence generated by the detection portion 301 .

[0135] The detection unit 301 is a component that obtains information based on a sample such as DNA that has been imbued with a fluorescent substance. Capillaries 312 are arranged and fixed to an optical plane near the detection unit 301 with a precision of several microns. During electrophoresis, a coaxial laser is irradiated from the light source 302. The irradiated laser continuously passes through all capillaries 312. This laser generates information light (fluorescence having a wavelength dependent on the sample) from the sample and is emitted to the outside from the detection unit 301. The optical detector 303 detects this information light. An analysis device (not shown) then analyzes the information light, thereby analyzing the sample.

[0136] (Autosampler 330)

[0137] The autosampler 330 is movable along three axes: vertical, horizontal, and depth. A cathode buffer container 332, a sample container 333, and the like are placed on a movable table 334 of the autosampler 330. This allows the autosampler 330 to transport the cathode buffer container 332, the sample container 333, and the like as needed. The sample container 333 contains a sample solution mixed with a sample.

[0138] (Control systems and processing systems)

[0139] The capillary electrophoresis device 300 is used while connected to a control computer 400 via a communication cable. An operator controls the functions of the capillary electrophoresis device 300 by operating the control computer 400. Furthermore, the control computer 400 can transmit and receive data detected by the detection unit 301 included in the capillary electrophoresis device 300. Furthermore, the control computer 400 can stop the capillary electrophoresis device 300.

[0140] exist Figure 7A In the illustrated example, the control computer 400 and the capillary electrophoresis apparatus 300 are separate apparatuses, but the control computer 400 may be integrated with the capillary electrophoresis apparatus 300 .

[0141] Furthermore, the processing device 1 obtains a source current value from the source ammeter 21 and a return current value from the return ammeter 22. Based on the obtained source and return current values, the processing device 1 detects discharge in the insulating portion 27 and abnormalities in the physical path 26. If discharge in the insulating portion 27 is detected, the processing device 1 outputs an error or instructs the control computer 400 to stop the capillary electrophoresis apparatus 300.

[0142] In addition, if Figure 7A As shown, the pull ammeter 21 is connected to the loading head 331. In addition, the return ammeter 22 is connected to the anode electrode 342A immersed in the buffer solution in the anode buffer container 342.

[0143] In addition, in the present embodiment, the processing device 1 and the control computer 400 are provided as separate devices, but the processing device 1 and the control computer 400 may be an integrated device.

[0144] In addition, the anode electrode 342A → the buffer solution contained in the anode buffer container 342 → the second tube 343b → the capillary head 321 → the capillary 312 → the loading head 331 is equivalent to Figure 1 The physical path shown is 26. In addition, Figure 1 The insulating portion 27 shown primarily corresponds to the gap between the loading head 331 and the cathode buffer container 332, or between the loading head and the buffer solution contained therein. The cathode buffer container 332 is position-controlled by the autosampler 330, but the distance between the cathode buffer container 332 and the loading head 331 may be increased due to a setting error or other factors. This condition can cause discharge between the loading head 331 and the cathode buffer container 332, or between the loading head and the buffer solution contained therein.

[0145] In addition, the loading head 331 is equivalent to Figure 1 The negative terminal 24, the anode electrode 342A is equivalent to Figure 1The positive terminal 25. In addition, Figure 1 In the embodiment, the positive terminal 25 is connected to the pull current meter 21 and the voltage source 23, and the negative terminal 24 is connected to the return current meter 22. Figure 7A In the example shown, the return current meter 22 is equivalent to Figure 1 The positive terminal 25 of the anode electrode 342A is connected. Figure 1 The negative terminal 24 of the loading head 331 is connected to the current meter 21 and the high voltage power supply 23A ( Figure 1 As described above, the configuration may be such that the source ammeter 21 is connected to the voltage source 23 side of the physical path 26, and the return ammeter 22 is connected to the opposite side. For example, the source ammeter 21 and the return ammeter 22 may be connected to either the negative terminal 24 or the positive terminal 25.

[0146] (Voltage Control Circuit)

[0147] Figure 8 2 is a diagram showing a voltage control circuit for performing voltage control in the capillary electrophoresis apparatus 300 .

[0148] The voltage control circuit includes a processing device 1, a control computer 400, a high-voltage power supply 23A, a source ammeter 21, and a return ammeter 22. The high-voltage power supply 23A applies voltage to the physical path 26 based on the control of the processing device 1. The high-voltage power supply 23A is equivalent to Figure 1 The voltage source 23. In addition, the physical path 26 is as described above.

[0149] The electrophoresis path is a flow path provided in the capillary array 311 , the interior of the block 323 , and a polymer filled in the second tube 343 b .

[0150] The high voltage power supply 23A is connected to the electrode 361 via the source ammeter 21, the hollow electrode 313 and the return ammeter 22. The electrode 361 is equivalent to Figure 1 The negative terminal 24 or Figure 7A When the high voltage power supply 23A applies a voltage of several tens of kilovolts to one end of the pull current meter 21, a voltage difference of several tens of kilovolts is generated between the pull current meter 21 and the electrode 361. At this time, an electric field is generated from the hollow electrode 313 toward the electrode 361. The sample, which is negatively charged by this electric field, is drawn from the cathode end 312A of the capillary 312 (refer to FIG. 3). Figure 7B ) moves toward the detection unit 301.

[0151] Then, the source ammeter 21 measures the source current value flowing from the high-voltage power supply 23A to the hollow electrode 313 and transmits the measured source current value to the processing device 1. In addition, the return ammeter 22 measures the return current value flowing from the electrode 361 to the GND and transmits the measured return current value to the processing device 1.

[0152] The processing device 1 reads the source current value from the source ammeter 21 and the return current value from the return ammeter 22, and performs calculations, i.e., an abnormality detection method. The processing device 1 then sends an instruction to the control computer 400 based on the results of the abnormality detection method. The control computer 400 forcibly shuts off the voltage of the high-voltage power supply 23A, thereby stopping the capillary electrophoresis device 300. Furthermore, the processing device 1 is capable of communicating with the control computer 400, which is located outside the capillary electrophoresis device 300.

[0153] Then, refer to Figure 7A and Figure 7B , the preparations before electrophoresis are explained.

[0154] Before starting measurement in the capillary electrophoresis apparatus 300 , the operator sets the following container in the capillary electrophoresis apparatus 300 .

[0155] • Anode buffer container 342 filled with buffer solution.

[0156] A cathode buffer container 332 is integrated with a waste liquid container for discharging a capillary cleaning liquid and polymer in the capillary 312 .

[0157] A polymer container 116 containing a polymer serving as a separation medium and a sample container 333 containing a sample to be measured.

[0158] The operator fills the anode buffer container 342 with enough buffer solution to fully immerse both the anode electrode 342A and the second tube 343b. The operator also checks that enough buffer solution is placed in the cathode buffer container 332 to fully immerse the hollow electrode 313 and the cathode end 312A of the capillary 312.

[0159] If the measurement is started without buffer solution, there is a risk of discharge between the high-potential cathode and other components with lower potentials when a high voltage is applied. In addition, the electrophoresis path or the flow path for transporting the polymer is completely filled with polymer before the measurement begins.

[0160] (Analysis and Processing)

[0161] Figure 9 1 is a flowchart showing an example of a process from the start of analysis to the end of analysis in the capillary electrophoresis apparatus 300. Figure 1 、 Figure 7A 、 Figure 7B .

[0162] The capillary electrophoresis device 300 starts analysis in response to a command sent from the control computer 400 ( S301 ).

[0163] Next, in preparation for injecting the polymer into the capillary 312 , the autosampler 330 mounted on the capillary electrophoresis apparatus 300 transports the cathode buffer container 332 to the cathode end 312A of the capillary 312 ( S302 ).

[0164] Thereafter, the polymer is injected into the capillary 312 by the pump mechanism 320 included in the capillary electrophoresis apparatus 300 ( S303 ).

[0165] In addition, the cathode end 312A of the capillary array 311 (capillary 312) is cleaned (S304).

[0166] Then, it is checked whether there is any abnormality in the capillary electrophoresis device 300. In checking whether there is any abnormality, the high-voltage power supply 23A applies a weak voltage (S305).

[0167] The processing device 1 then performs a current value check (S306) to determine whether an abnormality has occurred in the capillary electrophoresis device 300. The details of the current value check performed in step S306 will be described later. In step S306, the current value checked is the pull-through current value or the return current value. By performing the current value check at the stage of step S306, if an abnormality is detected at the stage of step S306, the operator can suspend subsequent processing. As a result, in the event of an abnormality, it is possible to prevent high voltage from being applied to the physical path 26, and to prevent waste of samples, etc.

[0168] When the processing device 1 determines that an abnormality has occurred as a result of the current value check (S306 → abnormality is present), the processing device 1 outputs an abnormality detection (S321). The abnormality detection output is an error output or an alarm output.

[0169] After step S321 , an abnormality response is performed ( S322 ). The abnormality response is a response by the operator or a stop of the capillary electrophoresis device 300 .

[0170] However, steps S321 and S322 may not be performed.

[0171] The weak voltage applied in step S305 is lower than the voltage applied by the high voltage power supply 23A during preliminary phoresis, sample introduction, and electrophoresis described later. However, the voltage applied in step S305 is several kV, which is generally considered to be high voltage.

[0172] At the stage of step S306 , the operator notices that an abnormality has occurred in the capillary electrophoresis device 300 , thereby reducing damage to components of the capillary electrophoresis device 300 .

[0173] In step S306, if the capillary electrophoresis apparatus 300 is operating normally (S306 → Normal), the high-voltage power supply 23A applies a predetermined voltage to the sample flow path, and the processing device 1 performs a preliminary electrophoresis run. During this run (during the preliminary electrophoresis run), a current value check is performed (S307). The preliminary electrophoresis run is used to prepare the polymer filling the capillary 312 for analysis before the actual electrophoresis analysis process, from sample introduction, begins. During the preliminary electrophoresis run, a voltage of several kilovolts to several tens of kilovolts is typically applied to the current path for several to several tens of minutes.

[0174] If the current check during the preliminary electrophoresis determines that the processing device 1 is abnormal (S307 → Abnormality Present), steps S321 and S322 are executed. By checking the current value in step S307, a current value check is performed before sample introduction. This allows abnormality determination of the capillary electrophoresis device 300 to be performed simultaneously with the preliminary electrophoresis. Furthermore, by performing abnormality detection of the capillary electrophoresis device 300 during the preliminary electrophoresis, electrophoresis can be suspended before sample introduction. This prevents sample waste.

[0175] When the preliminary electrophoresis is complete (the current test during the preliminary electrophoresis determines that the processing device 1 has no abnormalities: S307 → Normal), the cathode end 312A of the capillary 312 is cleaned with a buffer solution (S308). The autosampler 330 then transports the sample container 333 to the cathode end of the capillary 312 (S309).

[0176] Next, when a voltage of several kV is applied to the sample liquid contained in the sample container 333 by the high-voltage power supply 23A at the cathode electrode of the capillary 312, an electric field is generated between the sample liquid and the anode electrode. This electric field draws the sample in the sample liquid into the capillary 312. At this point, the processing device 1 checks the current value during sample introduction (S310).

[0177] If the current value check during sample introduction determines an abnormality (S310 → Abnormality), steps S321 and S322 are performed. By performing the current value check at step S310, the current value check is performed before electrophoresis. That is, abnormality detection of the capillary electrophoresis device 300 can be performed simultaneously with sample introduction. Furthermore, by performing abnormality detection of the capillary electrophoresis device 300 during sample introduction, electrophoresis can be suspended before electrophoresis.

[0178] When the introduction of the sample is completed (in the current check during the sample introduction, it is determined that the processing device 1 has no abnormality: S310 →normal), the cathode end 312A of the capillary 312 is cleaned with a buffer solution ( S311 ).

[0179] Next, the cathode buffer container 332 is transported to the cathode end 312A of the capillary 312 by the autosampler 330 (S312). The high-voltage power supply 23A then applies a predetermined voltage to the buffer solution contained in the cathode buffer container 332, thereby initiating electrophoresis. At this point, the voltage value during electrophoresis is checked (S313).

[0180] When it is determined that an abnormality is present in the voltage value inspection during electrophoresis ( S313 → abnormality present), the processes of steps S321 and S322 are performed.

[0181] During electrophoresis, the sample in the capillary 312 is given mobility by the action of the electric field generated between the cathode end 312A and the anode electrode 342A of the capillary 312. As a result, the sample is separated based on the difference in mobility that depends on the properties of the sample. The separated and moved samples are optically detected in sequence starting from the sample that reaches the detection unit 301. For example, if the sample is DNA, the mobility varies depending on the length of its bases, so the DNA with a shorter base length and a faster migration speed passes through the detection unit 301 in sequence. Since a fluorescent substance is pre-installed in the DNA, optical detection is performed by the detection unit 301. Generally, the measurement time and voltage application time are set according to the sample with the longest electrophoresis time.

[0182] like Figure 9 As shown in step S313, the current value check can also be performed during the electrophoresis stage. Furthermore, electrophoresis requires the application of high voltage for a long period of time. If a high voltage is continuously applied to the abnormal portion of the capillary electrophoresis device 300 while an abnormality is occurring, damage to the abnormal portion or surrounding components may occur. If an abnormality is detected in the capillary electrophoresis device 300 during electrophoresis, component damage can be avoided by stopping the capillary electrophoresis device 300 or notifying the operator of an error.

[0183] During the electrophoresis current value inspection, if the processing device 1 is determined to be normal (S313 → Normal), and a predetermined time has elapsed since the start of voltage application, the analysis device (not shown) completes acquisition of the predetermined data. High-voltage power supply 23A then stops applying voltage, and electrophoresis ends (S314). The analysis device analyzes the acquired data (electrophoresis results), completing the analysis.

[0184] The above is a series of measurement sequences.

[0185] The current value checks performed in steps S307 , S310 , and S313 can be omitted. However, by performing the current value checks in steps S307 , S310 , and S313 , it is possible to determine whether or not there is an abnormality in the capillary electrophoresis device 300 at each stage.

[0186] (Current value check)

[0187] Next, refer to Figure 10 The method for checking the current value is explained. Figures 10 to 16 The current value shown is checked at Figure 9 The process is performed in step S306 and then in S307, S310 and S314 as needed.

[0188] That is, in Figures 10 to 16 The current value check is performed before the preparatory electrophoresis of the capillary electrophoresis device 300 ( Figure 9 In step S306) of Figures 10 to 16 The current value inspection is performed at least once during the preparatory electrophoresis of the capillary electrophoresis device 300, during the sample introduction, and during electrophoresis. Figures 10 to 16 The current value inspection performed in the step includes a standard deviation calculation step, a first determination step, a second current value reading step, and a second determination step.

[0189] Figure 10 This is a flowchart showing an example of the process of checking the current value. Figures 10 to 16 In the description, refer to Figure 2 and Figure 7A .

[0190] Figure 10 The series of processing shown is to Figure 5 The processing shown is applied to the processing of the capillary electrophoresis system 3.

[0191] When voltage application ( Figure 9 When the current value is measured and the return current is measured, the current value is read (S401). Step S401 is the first current value reading step. Figure 7A In the case of the capillary electrophoresis system 3 shown in FIG. 1 , the return current meter 22 is connected to GND, so that almost no discharge occurs. Therefore, in the seventh embodiment, the value of the return current is not used. The source current value is obtained by Figure 4 The methods shown are sampled.

[0192] Next, the calculation unit 112 determines whether a sufficient amount, that is, a set number of times (according to Figure 4 The example shown is 10 times) of the source current value (S402).

[0193] When the source current value has not been read a set number of times ( S402 ), the processing device 1 returns the process to step S401 .

[0194] When the source current value has been read the set number of times ( S402 →Yes), the calculation unit 112 calculates the standard deviation per unit time using each of the read source current values ​​( S403 ). Step S403 is a standard deviation calculation step.

[0195] Then, the determination processing unit 113 determines whether the standard deviation per unit time is larger than a first threshold value ( S404 : first determination step).

[0196] In the case of the seventh embodiment, while the voltage is applied to the physical path 26, the processing device 1 reads and checks the fluctuation of the source current value at a cycle of 100 msec (see Figure 4 ). The calculation unit 112 calculates the standard deviation based on the current value obtained by sampling at several points (S403). Figure 4 In the example shown, the source current value is sampled at 10 points in 1 second, and the calculation unit 112 calculates the standard deviation of the sampled 10-point source current value. Thus, the calculation unit 112 calculates the standard deviation within 1 second (i.e., unit time). Figure 3A to Figure 3C As described in the insulating portion 27 (ie, Figure 7A If discharge occurs between the loading head 331 and the cathode buffer container 332 or the buffer solution, the drawn current value will have unevenness (standard deviation). Therefore, the standard deviation of the drawn current value becomes larger than when no discharge occurs in the insulating portion 27.

[0197] Therefore, in the seventh embodiment, a first threshold value is set as a certain threshold value, and the determination processing unit 113 determines whether the standard deviation calculated in step S403 is greater than the first threshold value (S404). The first threshold value is determined by the operator. For example, parameters that affect the source current value are considered, such as the type of capillary electrophoresis device 300 (CCE, 3500, etc.), the length of the capillary 312, the number of capillaries 312, and the type of polymer used.

[0198] In step S404, if the standard deviation exceeds the first threshold value (S404 → Yes), the determination processing unit 113 determines whether the current state has just been changed in the applied voltage (S406: exclusion step). If the applied voltage has just been changed (S406 → Yes), the determination processing unit 113 determines that the change in the source current value is not due to discharge in the insulating portion 27. This is because, as described above, immediately after the applied voltage has been changed, the source current value (and the return current value) fluctuates in accordance with Ohm's law.

[0199] Therefore, if the applied voltage has just been changed (S406→Yes), the determination is left (S407: exclusion step). Thus, if the applied voltage has just been changed, the measured source current value is excluded from the discharge determination of the insulating portion 27.

[0200] For example, the current drawn value for 3 seconds (100 msec x 30 points) after the applied voltage is changed is excluded from the discharge determination of the insulating portion 27. The operator determines the length of time immediately after the applied voltage is changed that is not used for discharge determination. For example, parameters that affect the current drawn value are considered, such as the type of capillary electrophoresis device 300 (CCE, 3500, etc.), the length of the capillary 312, the number of capillaries 312, and the type of polymer used.

[0201] If the result of step S406 indicates that the applied voltage has not been changed immediately (S406 → No), the determination processing unit 113 determines that discharge has occurred in the insulating portion 27 (S408). In this case, the control processing unit 114 may stop the capillary electrophoresis device 300, and the output processing unit 115 may output an error or alarm.

[0202] On the other hand, in step S404 , when the value of the standard deviation is equal to or smaller than the first threshold value ( S404 →No), it is determined that discharge has not occurred in the insulating portion 27 ( S408 ).

[0203] According to the seventh embodiment, the abnormality detection method performed in the first embodiment can be applied to the capillary electrophoresis system 3 .

[0204] [Eighth Embodiment]

[0205] Next, refer to Figure 11 An eighth embodiment of the present invention will be described.

[0206] Figure 11 This is a flowchart showing an example of the processing procedure of the eighth embodiment.

[0207] The seventh embodiment described above describes a detection method for determining whether discharge occurs in the insulating portion 27 in the capillary electrophoresis device 300. The eighth embodiment describes stopping the capillary electrophoresis device 300 as a response method when discharge occurs in the insulating portion 27.

[0208] In this embodiment, if Figure 11As shown, if the determination in step S406 is "No," the control processing unit 114 forcibly stops the capillary electrophoresis device 300 (S408A: stop control processing step). In other words, upon detecting discharge in the insulating portion 27, the control processing unit 114 forcibly stops the capillary electrophoresis device 300, which is the abnormality monitoring target device 2.

[0209] In addition, if it is determined as "No" in step S404 or "Yes" in step S406, electrophoresis is continued (S411). Figure 10 In the case of determining that the discharge is retained (S407) or "no discharge" (S408), the capillary electrophoresis device 300 does not stop but continues the electrophoresis. Figure 9 The processing shown.

[0210] The eighth embodiment differs from the seventh embodiment in the aforementioned respects, but is otherwise identical to the seventh embodiment. According to the eighth embodiment, upon determining that a discharge has occurred in the insulating portion 27, the control processing unit 114 forcibly stops the capillary electrophoresis device 300. This allows the processing device 1 to detect the discharge in the insulating portion 27 and safely stop the capillary electrophoresis device 300. Furthermore, since an operator cannot move the capillary electrophoresis device 300 while a discharge has occurred in the insulating portion 27, damage to the components of the capillary electrophoresis device 300 can be prevented.

[0211] [Ninth embodiment]

[0212] Next, refer to Figure 12 A ninth embodiment of the present invention will be described.

[0213] Figure 12 This is a flowchart showing an example of the processing procedure of the ninth embodiment.

[0214] In the eighth embodiment described above, the control processing unit 114 stops the capillary electrophoresis device 300 when discharge occurs in the insulating portion 27. In contrast, in the ninth embodiment, the output processing unit 115 outputs an error instead of stopping the capillary electrophoresis device 300.

[0215] In this embodiment, when the determination result in step S406 is “No”, the output processing unit 115 causes the output device 15 ( Figure 2 ) Output error (S408B: error output step). After that, the operator takes action (S421).

[0216] The ninth embodiment differs from the eighth embodiment in the aforementioned respects, but is otherwise identical to the eighth embodiment. In the ninth embodiment, upon detecting discharge in the insulating portion 27, the output processing unit 115 outputs an error output to the output device 15. This allows the operator to be aware that discharge is occurring in the insulating portion 27 of the capillary electrophoresis device 300.

[0217] [Tenth embodiment]

[0218] Next, refer to Figure 13 A tenth embodiment of the present invention will be described.

[0219] Figure 13 This is a flowchart showing an example of a processing procedure of the tenth embodiment.

[0220] In the ninth embodiment described above, the output processing unit 115 outputs an error when a discharge occurs in the insulating portion 27. In the tenth embodiment, an alarm is output as a specific example of an output error (S408C). While the tenth embodiment differs from the ninth embodiment in this respect, it is otherwise identical to the ninth embodiment. In the tenth embodiment, if it is determined that the insulating portion 27 is discharging, the output processing unit 115 outputs an alarm as an error output. This allows the operator to be aware that the insulating portion 27 of the capillary electrophoresis device 300 is discharging.

[0221] [Eleventh embodiment]

[0222] Next, refer to Figure 14 An eleventh embodiment of the present invention will be described.

[0223] Figure 14 This is a flowchart showing an example of a processing procedure of the eleventh embodiment.

[0224] The seventh embodiment described above detects only the discharge of the insulating portion 27 by measuring the pull current value. In the eleventh embodiment, there is an abnormality detection process of returning the current value, namely S501 to S505. Figures 14 to 16 In the flowchart shown, the return current meter 22 is not connected to GND.

[0225] That is, the return current value is read by the return current meter 22 ( S501 : second current value reading step).

[0226] Next, the calculation unit 112 calculates the change value of the return current value (S502). Specifically, the determination processing unit 113 calculates the difference between the return current value read last time and the return current value read this time.

[0227] Then, the determination processing unit 113 determines whether the change value of the return current value is larger than the second threshold value ( S503 : second determination step).

[0228] If the change in the return current value exceeds the second threshold (S503 → Yes), the determination processing unit 113 determines that an abnormality has occurred in the physical path 26, and the output processing unit 115 outputs an error (S504: second determination step). An abnormality in the physical path 26 refers to discharge or poor conduction caused by, for example, bubbles entering the flow path. After step S504, the operator performs measures such as removing the bubbles (S505).

[0229] If the change value of the return current value is less than the second threshold value (S503→No), the current value is read by the current meter 21 (S401). Figure 10 The same, so the description is omitted.

[0230] The eleventh embodiment differs from the seventh embodiment in that steps S501 to S505 are performed, but is the same as the seventh embodiment in other respects. The eleventh embodiment only detects discharge detection by measuring the pull current value. In contrast, in the eleventh embodiment, the abnormality detection process of the return current value shown in steps S501 to S504 is performed. In addition, the abnormality of the physical path 26 detected based on the change in the return current value refers to discharge and poor conduction caused by bubbles and garbage mixed in the flow path of the capillary 312, etc. The eleventh embodiment differs from the seventh embodiment in that steps S501 to S504 are performed, but is the same as the seventh embodiment in other respects. By performing steps S501 to S504, when bubbles and garbage enter the flow path of the sample, the abnormality of the return current value from the flow path (physical path 26) is output as an error (S504). Then, the operator can take measures such as bubble removal (S505).

[0231] [Twelfth embodiment]

[0232] Next, refer to Figure 15 A twelfth embodiment of the present invention will be described.

[0233] Figure 15 This is a flowchart showing an example of the processing procedure of the twelfth embodiment.

[0234] In the eleventh embodiment, a method for detecting whether an abnormality occurs in the physical path 26 is described. In the twelfth embodiment, when an electric discharge occurs in the insulating portion 27, that is, when it is determined to be "No" in step S406, the control processing unit 114 forcibly stops the capillary electrophoresis device 300 (S408A). In addition, when it is determined to be "No" in step S404 or "Yes" in step S406, electrophoresis is continued (S411). In these aspects, the twelfth embodiment is different from the eleventh embodiment, but the other aspects are the same as the eleventh embodiment. Thus, in the twelfth embodiment, when it is determined that an electric discharge occurs in the insulating portion 27, the control processing unit 114 forcibly stops the capillary electrophoresis device 300. Thus, the operator will not move the capillary electrophoresis device 300 in the state of discharge, thereby preventing damage to the components of the capillary electrophoresis device 300.

[0235] [Thirteenth embodiment]

[0236] Next, refer to Figure 16 A thirteenth embodiment of the present invention will be described.

[0237] Figure 16 This is a flowchart showing an example of a processing procedure of the thirteenth embodiment.

[0238] The twelfth embodiment described above describes a method in which the control processing unit 114 stops the capillary electrophoresis device 300 when discharge occurs in the insulating portion 27. In the thirteenth embodiment, an error is output instead of stopping the capillary electrophoresis device 300.

[0239] That is, in the thirteenth embodiment, when discharge occurs in the insulating portion 27, that is, when the determination in step S406 is "No", the output processing unit 115 outputs an error (S408B). Thereafter, the operator takes action (S421).

[0240] The thirteenth embodiment differs from the twelfth embodiment in the above respects, but is otherwise the same as the twelfth embodiment. In the thirteenth embodiment, when it is determined that the insulating portion 27 is discharged, an error is output, allowing the operator to notice that discharge has occurred in the insulating portion 27.

[0241] [Screen example]

[0242] Figure 17 1 is a diagram showing an example of the abnormality detection screen 500 .

[0243] like Figure 17As shown, the abnormality detection screen 500 includes a source current value display portion 501 , a standard deviation display portion 502 , an insulation portion detection result display portion 503 , and a physical path detection result display portion 504 .

[0244] The current value display unit 501 displays the Figure 5 The source current value read in step S101 etc. In addition, in the source current value display unit 501, as shown in FIG. Figure 17 As shown, a period T11 not used for discharge detection of the insulating portion 27 and a period T12 used can be displayed.

[0245] Furthermore, the standard deviation display unit 502 displays Figure 5 The standard deviation calculated in step S103 and the like is displayed in the insulation part detection result display unit 503. Figure 5 That is, the occurrence of discharge in the insulating portion 27 is outputted in the insulating portion detection result display unit 503 .

[0246] In addition, the physical path detection result display unit 504 displays Figure 6 That is, the occurrence of an abnormality in the physical path 26 is outputted in the physical path detection result display unit 504 .

[0247] The present invention is not limited to the above-described embodiments and includes various variations. For example, the above-described embodiments are described in detail to facilitate understanding of the present invention and are not necessarily limited to having all the structures described. In addition, a portion of the structure of a certain embodiment can be replaced with the structure of another embodiment, and the structure of another embodiment can be added to the structure of a certain embodiment. In addition, with respect to a portion of the structure of each embodiment, other structures can be added, deleted, or replaced.

[0248] In addition, in this embodiment Figure 5 、 Figure 6 、 Figures 10 to 16 In each flowchart in FIG, after calculating the standard deviation, it is determined whether the current state is immediately after the voltage has changed. However, the present invention is not limited to this, and the standard deviation may be calculated after determining that the current state is not immediately after the voltage has changed.

[0249] In addition, the above-mentioned structures, functions, processing unit 100, current value acquisition unit 111 to output processing unit 115, storage device 13, etc. can also be implemented by hardware by designing part or all of them, for example, using integrated circuits. Figure 2As shown, the above-mentioned structures and functions can also be implemented by software by a processor such as a CPU interpreting and executing programs that implement each function. In addition to being stored in an HD (Hard Disk), information such as programs, tables, and files that implement each function can also be stored in a recording device such as the memory 11, an SSD (Solid State Drive), or a recording medium such as an IC (Integrated Circuit) card, an SD (Secure Digital) card, or a DVD (Digital Versatile Disc).

[0250] In addition, in each embodiment, the control lines and information lines are shown as lines considered necessary for explanation, and not all control lines and information lines are shown in the product. In reality, it can be considered that almost all components are connected to each other.

[0251] Explanation of symbols

[0252] 1. Processing device (abnormality detection device)

[0253] 2. Devices subject to abnormal monitoring

[0254] 3 Capillary electrophoresis system

[0255] 15 output device (output unit)

[0256] 21 pull current meter (first current meter)

[0257] 22 Return current meter (second current meter)

[0258] 23 Voltage Source

[0259] 23A high voltage power supply

[0260] 24 negative terminal

[0261] 25 positive terminal

[0262] 26 physical paths (first path)

[0263] 27 Insulation portion (second path)

[0264] 110 Processing Department

[0265] 111 Current value acquisition unit

[0266] 112 Computing Department

[0267] 113 Judgment Processing Unit

[0268] 114 Control Processing Unit

[0269] 115 Output Processing Unit

[0270] 300 capillary electrophoresis device

[0271] 301 Inspection Department

[0272] 302 light source

[0273] 303 optical detector

[0274] 311 capillary array

[0275] 312 capillary

[0276] 312A cathode end

[0277] 313 hollow electrode

[0278] 331 loading head

[0279] 332 cathode buffer container

[0280] 333 sample container

[0281] 342 anode buffer container

[0282] 342A anode electrode

[0283] 343a First Tube

[0284] 343b second tube

[0285] 361 electrode

[0286] 400 control computer

[0287] 500 abnormality detection screen

[0288] 501 source current value display unit

[0289] 502 standard deviation display unit

[0290] 503 insulation part test result display

[0291] 504 Physical path detection result display unit

[0292] During T11

[0293] During T12

[0294] Z Anomaly Detection System

[0295] S101: Reading the source current value (first current value reading step)

[0296] S103, S403 Standard Deviation Calculation (Standard Deviation Calculation Steps)

[0297] S104, S404 Comparison of standard deviation with first threshold value (first determination step)

[0298] S106 and S406 determine whether the voltage has just been changed (elimination step)

[0299] S107, S407 reserved (elimination step)

[0300] S408A Capillary Electrophoresis Device Stop (Stop Control Processing Step)

[0301] S408B Error Output (Error Output Step)

[0302] S201 returns to current value reading (second current value reading step)

[0303] S203 Returns the comparison of the current value with the second threshold value (second determination step)

[0304] S306 Current value check (standard deviation calculation step, first determination step, second current value reading step, and second determination step performed before preparatory electrophoresis)

[0305] S307 Current Value Check (Standard Deviation Calculation Step, First Determination Step, Second Current Value Reading Step, and Second Determination Step Performed During Preparatory Phosphoresis)

[0306] S310 Current Value Check (Standard Deviation Calculation Step, First Judgment Step, Second Current Value Reading Step, and Second Judgment Step Performed During Sample Introduction)

[0307] S313 Current Value Check (Standard Deviation Calculation Step, First Judgment Step, Second Current Value Reading Step, and Second Judgment Step Performed During Electrophoresis)

[0308] S401: The source current value is read (first current value reading step).

Claims

1. A method for detecting anomalies, characterized in that: An abnormality detection device that reads a first current value from a first ammeter connected in series with a first path between a first path connected by a voltage applied by a voltage source and the voltage source performs the following steps: a standard deviation calculating step of calculating the standard deviation of the first current value; and In the first determination step, when the standard deviation is larger than a first threshold value that is a predetermined threshold value, outputting that discharge has occurred in a second path that is a path other than the first path.

2. The anomaly detection method according to claim 1, wherein: The abnormality detection device executes a first current value reading step of reading the first current value at predetermined time intervals, In the standard deviation calculation step, the standard deviation is calculated based on the first current value read in at a predetermined sampling cycle.

3. The anomaly detection method according to claim 1, wherein: The abnormality detection device executes an exclusion step of excluding the first current value read within a predetermined period after the voltage applied to the voltage source is changed from being a target for determination of the discharge.

4. The anomaly detection method according to claim 1, wherein: The abnormality detection device executes a stop control processing step of stopping the abnormality monitoring target device provided with the first path when the occurrence of the discharge is detected.

5. The anomaly detection method according to claim 1, wherein: The abnormality detection device performs an error output step of performing an error output on an output unit when the discharge is detected.

6. The anomaly detection method according to claim 5, characterized in that: The error output is an output of an alarm.

7. The anomaly detection method according to claim 1, wherein: The anomaly detection device performs the following steps: a second current value reading step of reading a second current value from a second ammeter connected in series with the first path and the voltage source on the side opposite to the voltage source via the first path; as well as In the second determination step, a variation value of the second current value is calculated, and when the variation value is larger than a second threshold value which is a predetermined threshold value, an output is given that an abnormality has occurred in the first path.

8. The anomaly detection method according to claim 1, wherein: The abnormality monitoring target device provided with the first path is a capillary electrophoresis device, The first ammeter is connected to the loading head.

9. The anomaly detection method according to claim 8, characterized in that: The standard deviation calculation step and the first determination step are performed before the preliminary electrophoresis of the capillary electrophoresis apparatus.

10. The anomaly detection method according to claim 9, characterized in that: The standard deviation calculation step and the first determination step are performed at at least one of a preliminary run, a sample introduction, and electrophoresis in the capillary electrophoresis apparatus.

11. The anomaly detection method according to claim 8, wherein: In the anode buffer container, the anode electrode immersed in the buffer solution is connected to a second ammeter. The anomaly detection device performs the following steps: a second current value reading step of reading a second current value from the second ammeter; as well as In the second determination step, a variation value of the second current value is calculated, and when the variation value is larger than a second threshold value which is a predetermined threshold value, an output is given that an abnormality has occurred in the first path.

12. The abnormality detection method according to claim 11, characterized in that: The second current value reading step and the second determination step are performed before the preliminary electrophoresis of the capillary electrophoresis apparatus.

13. The abnormality detection method according to claim 11, characterized in that: The second current value reading step and the second determination step are performed at at least one of a preliminary electrophoresis time, a sample introduction time, and an electrophoresis time of the capillary electrophoresis device.

14. An abnormality detection device, characterized in that: have: a calculation unit that calculates a standard deviation of a first current value read from a first ammeter connected in series with a first path and the voltage source, between the first path conducted by a voltage applied by a voltage source and the voltage source; as well as The determination processing unit outputs that discharge has occurred in a second path that is a path other than the first path, when the standard deviation is larger than a first threshold value that is a predetermined threshold value.

Images