Vinasse seedling substrate and preparation process thereof

Abstract

The invention relates to a vinasse seedling substrate and a preparation process thereof. The vinasse seedling substrate comprises the following components in parts by weight: 40-60 parts of vinasse, 25-35 parts of coco coir, 7-12 parts of perlite, 7-12 parts of humic acid, 5-15 parts of bacterium-loaded bamboo fibers and 0.4-0.8 part of ferroferric oxide particles. The preparation method comprises the following steps: S1, carrying out fermentation treatment on the vinasse; s2, vinasse, coco coir and humic acid are put into a double-shaft stirrer to be preliminarily mixed, and a preliminarily mixed matrix is formed; s4, the primarily mixed matrix, perlite, bacterium-loaded bamboo fibers and ferroferric oxide particles are put into a three-dimensional mixing machine and mixed for 30 min at the rotating speed of 20-30 rpm, the water content is adjusted to be 30-40%, and the pH value is adjusted to be 6.0-7.0; and S5, carrying out magnetization treatment on the ferroferric oxide particles. The seedling substrate disclosed by the invention has relatively good air permeability and nutritional ingredients, and is also beneficial to promoting the growth of root systems.

Description

Technical Field

[0001] The invention belongs to the technical field of seedling culture medium preparation, and in particular relates to a distiller's grains seedling matrix and a preparation process thereof. Background Art

[0002] As China's baijiu (white spirits) industry continues to expand on a large scale, the annual production of its byproduct, distiller's grains, is estimated to have exceeded 20 million tons and continues to grow. Improper disposal of such a massive amount of distiller's grains would pose significant environmental pressures. However, research has revealed that distiller's grains contain a high-value biological resource, rich in crude protein, essential amino acids, B vitamins, and minerals such as calcium, phosphorus, potassium, and magnesium. This unique system of bioactive ingredients offers significant nutritional advantages.

[0003] The current mainstream seedling substrate is mainly composed of materials such as peat (organic material) and vermiculite (inorganic material), supplemented by ingredients such as bagasse and decomposed compost. This type of substrate has a dense physical structure and generally low aeration porosity, which can easily lead to insufficient oxygen exchange efficiency at the roots and cause root rot.

[0004] Therefore, there is an urgent need to develop a new seedling cultivation medium that uses distiller's grains as the main ingredient and other auxiliary materials through a special process. This medium must have high air permeability and nutrient content to be suitable for seedling cultivation and can effectively promote the growth of the seedling root system. Summary of the Invention

[0005] The invention provides a distiller's grains seedling matrix and a preparation process thereof. The seedling matrix can provide high air permeability and nutrients and can effectively promote the growth of seedling roots.

[0006] The technical solution adopted by the present invention is:

[0007] A distiller's grains seedling matrix comprises, by weight, 40-60 parts of distiller's grains, 25-35 parts of coconut bran, 7-12 parts of perlite, 7-12 parts of humic acid, 5-15 parts of bacteria-laden bamboo fibers, and 0.4-0.8 parts of ferroferric oxide particles.

[0008] Furthermore, it includes 50 parts of wine lees, 30 parts of coconut bran, 10 parts of perlite, 10 parts of humic acid, 10 parts of bacteria-loaded bamboo fiber and 0.6 parts of ferrosoferric oxide particles.

[0009] Furthermore, the lees are white wine lees.

[0010] A preparation process for distiller's grains seedling matrix comprises the following steps:

[0011] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0012] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 20-30 rpm for 20 min to form a primary mixed matrix;

[0013] S4. Add the primary mixed matrix, perlite, bacteria-loaded bamboo fiber, and ferroferric oxide particles into a three-dimensional mixer and mix at 20-30 rpm for 30 min. During the mixing process, water and limestone are added to adjust the water content of the mixed matrix to 35-45% and the pH value to 6.0-7.0.

[0014] S5. Magnetizing the ferroferric oxide particles.

[0015] Furthermore, the specific steps of fermentation treatment of wine lees include:

[0016] S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 2-5 kGy and a process temperature of ≤40°C;

[0017] S12a. Adjust the moisture content of the lees to ≤40%, add EM bacterial agent at 0.5%-1% of the weight of the lees, mix well, and then ferment for 7-10 days, maintaining the temperature ≤40°C during the process.

[0018] Furthermore, the step of demagnetizing the ferroferric oxide particles includes:

[0019] S11b, placing the ferroferric oxide particles in a demagnetizer, and treating them at a frequency of 0.5-2 Hz and a magnetic field strength of 2000-3000 Oersteds for 3-5 minutes;

[0020] S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

[0021] Furthermore, the particle size of the ferrosoferric oxide particles is 0.4-0.6 mm.

[0022] Furthermore, the ferroferric oxide is treated by the following steps before demagnetization:

[0023] S11c, dissolving low-density polyethylene in xylene solvent to prepare a 10-15 wt% low-density polyethylene solution;

[0024] S12c, preheating the ferrosoferric oxide particles to 80-100° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.2-1.5 m / s to disperse them;

[0025] S13c, at a fluidized bed temperature of 120-130° C., spraying a low-density polyethylene solution at a rate of 2-4 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of the sprayed liquid being 8-12% of the mass of the iron powder;

[0026] S14c. Maintaining the inert gas flow rate, reduce the fluidized bed temperature to below 25° C. at a rate of 3-7° C. / min, and allow the ferroferric oxide particles to be dispersed by air jets in the fluidized bed for ≥30 min. Pass the coated ferroferric oxide particles through a 20-mesh sieve.

[0027] Furthermore, the magnetization treatment method of the ferroferric oxide is: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 5000-7000 Oersteds, a pulse frequency of 3-5 times / s, and a duration of 60-90s.

[0028] Furthermore, the preparation steps of the bacteria-loaded bamboo fiber include the following:

[0029] S41a. Crush the bamboo fiber into pieces of 0.8-1.2 mm, soak it in 3-4% hydrogen peroxide for 30 minutes to remove impurities, and dry it to a moisture content of ≤5%.

[0030] S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, and the live bacterial concentration of the composite bacterial solution is ≥ 5×10 9 CFU / mL;

[0031] S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 3-5% of the bamboo fiber mass, fermenting at 30-35°C and 55-60% humidity for 7 days, stirring once every 24 hours;

[0032] S44a, drying at 40-45° C. until the moisture content is ≤10%, to obtain bacteria-laden bamboo fiber.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. Good air permeability: Through the rational ratio of perlite and coconut coir, their porous structure and the fiber network formed after the fermentation of distiller's grains form a three-dimensional interconnected pore system. When mixed with 2-3mm perlite, it can provide a high natural porosity. Secondly, the demagnetized ferroferric oxide particles are first evenly mixed into the matrix. The matrix, which is then adjusted to an appropriate humidity, is then placed in a magnetic field. The magnetic field can induce the matrix particles to align in a certain direction, which is beneficial for optimizing pore connectivity and thus increasing porosity. At the same time, the ferroferric oxide particles are magnetized as a subsequent magnetic field source.

[0035] 2. High nutrient matrix:

[0036] The main ingredient, baijiu lees, undergoes irradiation sterilization and microbial fermentation. Leveraging the abundant nutrients within the lees, this treatment decomposes macromolecules and releases bound nutrients, forming a nutrient reservoir comprised of fast-acting small-molecule organic matter, inorganic salts, slow-acting humic substances, and some recalcitrant organic matter. Humic acid, a natural chelating agent, contains functional groups such as carboxyl and phenolic hydroxyl groups that react with trace element ions such as iron, manganese, zinc, and copper to form highly stable organic-metal chelates. This prevents trace elements from being fixed in the matrix while allowing for a slow release of nutrients through the gradual decomposition of the chelate, significantly increasing their bioavailability. Combined with bacterial-loaded bamboo fiber, which is loaded with functional microorganisms such as Bacillus subtilis and Bacillus mucilaginosus, the fiber continuously secretes a variety of metabolites, including organic acids, iron carrier proteins, and auxin-like substances, during the subsequent cultivation and fermentation process. These metabolites can acidify the matrix environment, dissolve insoluble phosphates and metal oxides, and activate fixed nutrients through chelation, forming a dynamic nutrient supply system of "microbial activation-metabolite conversion-humic acid stabilization", ensuring that the seedlings receive balanced nutritional support throughout the growth cycle.

[0037] 3. Promote vascular development and rooting:

[0038] This invention utilizes physical-biological synergistic stimulation to enhance the physiological activity of seedlings. During the fermentation process, the Bacillus subtilis and Bacillus mucilaginosus bacteria loaded into the bamboo fiber continuously secrete various plant growth regulators. These, combined with the weak magnetic field generated by the magnetized ferroferric oxide particles, create a synergistic effect: the growth regulators induce vascular cell division and differentiation. Combined with the magnetic field, they modulate cell membrane potential and ion channel activity, accelerating proton pump operation and material transport. The two synergistically shorten the period of new root primordium formation, effectively promoting the development of the seedling's vascular bundle and root growth. DETAILED DESCRIPTION

[0039] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0040] Example 1

[0041] A distiller's grains seedling matrix comprises, by weight, 40 parts of distiller's grains, 25 parts of coconut bran, 7 parts of perlite, 7 parts of humic acid, 5 parts of bacteria-loaded bamboo fiber and 0.4 parts of ferroferric oxide particles, wherein the distiller's grains are white wine distiller's grains.

[0042] A preparation process of a distiller's grains seedling matrix comprises the following steps:

[0043] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0044] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 20 rpm for 20 min to form a primary mixed matrix;

[0045] S4, adding the primary mixed matrix, perlite, bacteria-loaded bamboo fiber and ferrosoferric oxide particles into a three-dimensional mixer, mixing at 20 rpm for 30 min, and adding water and limestone during the stirring process to make the water content of the mixed matrix 35% and the pH value 6.0;

[0046] S5. Magnetizing the ferroferric oxide particles.

[0047] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0048] S11a, irradiating the wine lees with an electron beam for sterilization, with an irradiation dose of 2 kGy and a process temperature controlled at 35°C;

[0049] S12a, adjusting the moisture content of the lees to 35%, adding EM bacterial agent at a rate of 0.5% of the weight of the lees, mixing well, and then fermenting for 7 days, maintaining the temperature at 35°C during the process.

[0050] Preferably, the step of demagnetizing the ferroferric oxide particles comprises:

[0051] S11b, placing the ferroferric oxide particles in a demagnetizer and treating them at a frequency of 0.5 Hz and a magnetic field strength of 2000 Oersteds for 3 minutes;

[0052] S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

[0053] Preferably, the particle size of the ferrosoferric oxide particles is 0.4 mm.

[0054] Preferably, the ferroferric oxide is treated by the following steps before demagnetization:

[0055] S11c, dissolving low-density polyethylene in xylene solvent to prepare a 10 wt% low-density polyethylene solution;

[0056] S12c, preheating the ferrosoferric oxide particles to 80° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.2 m / s to disperse them;

[0057] S13c, at a fluidized bed temperature of 120°C, spray a low-density polyethylene solution at a rate of 2 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of sprayed liquid being 8% of the mass of the iron powder;

[0058] S14c. Maintaining the inert gas flow rate, lower the fluidized bed temperature to below 20° C. at a rate of 3° C. / min, allowing the ferroferric oxide particles to be dispersed by air jets in the fluidized bed for 30 minutes, and passing the coated ferroferric oxide particles through a 20-mesh sieve.

[0059] Preferably, the magnetization treatment method of ferroferric oxide is: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 6000 Oersteds, a pulse frequency of 3 times / s, and a duration of 60s.

[0060] Preferably, the preparation steps of the bacteria-loaded bamboo fiber include the following:

[0061] S41a, crushing the bamboo fiber into 0.8 mm, soaking it in 3% hydrogen peroxide for 30 minutes to remove impurities, and drying it to a moisture content of 5%.

[0062] S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, with a live bacterial concentration of 5×10 9 CFU / mL;

[0063] S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 3% of the bamboo fiber mass, and fermenting at 30°C and 55% humidity for 7 days, stirring once every 24 hours;

[0064] S44a, drying at 40° C. to a moisture content of 5% to obtain bacteria-laden bamboo fiber.

[0065] Example 2

[0066] A distiller's grains seedling matrix comprises, by weight, 60 parts of distiller's grains, 35 parts of coconut bran, 12 parts of perlite, 12 parts of humic acid, 15 parts of bacteria-loaded bamboo fiber, and 0.8 parts of ferroferric oxide particles, wherein the distiller's grains are white wine distiller's grains.

[0067] A preparation process for distiller's grains seedling matrix comprises the following steps:

[0068] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0069] S2, put the wine grains, coconut husk and humic acid into a twin-shaft mixer and mix at 30 rpm for 20 min to form a primary mixed matrix;

[0070] S4, adding the primary mixed matrix, perlite, bacteria-loaded bamboo fiber and ferrosoferric oxide particles into a three-dimensional mixer, mixing at 30 rpm for 30 min, and adding water and limestone during the stirring process to make the water content of the mixed matrix 45% and the pH value 7.0;

[0071] S5. Magnetizing the ferroferric oxide particles.

[0072] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0073] S11a, sterilizing the lees by irradiation with an electron beam, with an irradiation dose of 5 kGy and a process temperature of 37°C;

[0074] S12a. Adjust the moisture content of the lees to 37%, add EM bacterial agent at 1% of the weight of the lees, mix well, and then ferment for 10 days while maintaining the temperature at 37°C.

[0075] Preferably, the step of demagnetizing the ferroferric oxide particles comprises:

[0076] S11b, placing the ferroferric oxide particles in a demagnetizer and treating them at a frequency of 2 Hz and a magnetic field strength of 3000 Oersteds for 5 minutes;

[0077] S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

[0078] Preferably, the particle size of the ferrosoferric oxide particles is 0.6 mm.

[0079] Preferably, the ferroferric oxide is treated by the following steps before demagnetization:

[0080] S11c, dissolving low-density polyethylene in xylene solvent to prepare a 15 wt% low-density polyethylene solution;

[0081] S12c, preheating the ferrosoferric oxide particles to 100° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.5 m / s to disperse them;

[0082] S13c, at a fluidized bed temperature of 130°C, spraying a low-density polyethylene solution at a rate of 4 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of the sprayed liquid being 12% of the mass of the iron powder;

[0083] S14c. Maintaining the inert gas flow rate, lower the fluidized bed temperature to below 25° C. at a rate of 7° C. / min, and then disperse the ferroferric oxide particles in the fluidized bed by air jet for 33 minutes. Pass the coated ferroferric oxide particles through a 20-mesh sieve.

[0084] Preferably, the magnetization treatment method of ferroferric oxide is: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 7000 Oersteds, a pulse frequency of 5 times / s, and a duration of 90s.

[0085] Preferably, the preparation steps of the bacteria-loaded bamboo fiber include the following:

[0086] S41a, crushing the bamboo fiber into 1.2 mm, soaking it in 4% hydrogen peroxide for 30 minutes to remove impurities, and drying it to a moisture content of 5%.

[0087] S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, with a live bacterial concentration of 5×10 9 CFU / mL;

[0088] S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 5% of the bamboo fiber mass, fermenting at 35°C and 60% humidity for 7 days, stirring once every 24 hours;

[0089] S44a, drying at 45° C. to a moisture content of 10% to obtain bacteria-laden bamboo fiber.

[0090] Example 3

[0091] A distiller's grains seedling matrix comprises, by weight, 50 parts of distiller's grains, 30 parts of coconut bran, 10 parts of perlite, 10 parts of humic acid, 10 parts of bacteria-loaded bamboo fiber, and 0.6 parts of ferroferric oxide particles, wherein the distiller's grains are white wine distiller's grains.

[0092] A preparation process of a distiller's grains seedling matrix comprises the following steps:

[0093] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0094] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 25 rpm for 20 min to form a primary mixed matrix;

[0095] S4, adding the primary mixed matrix, perlite, bacteria-loaded bamboo fiber and ferrosoferric oxide particles into a three-dimensional mixer, mixing at 25 rpm for 30 min, and adding water and limestone during the stirring process to make the water content of the mixed matrix 37% and the pH value 6.5;

[0096] S5. Magnetizing the ferroferric oxide particles.

[0097] First, the ferroferric oxide particles are demagnetized to prevent the ferroferric oxide particles from agglomerating during the mixing process, and then the ferroferric oxide particles uniformly mixed in the matrix are magnetized as a whole. Combined with the appropriate humidity of the matrix, it is suitable for cultivating seedlings while also keeping the magnetized ferroferric oxide particles uniformly in the matrix. The magnetized ferroferric oxide particles can provide a uniform micromagnetic field for the seedlings planted in the matrix, which is beneficial to promoting the uniform growth of new roots of the seedlings around the main stem, and preventing the new roots from growing only on one side and affecting the nutrient absorption effect.

[0098] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0099] S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 3.5 kGy and a process temperature of 40°C;

[0100] S12a. Adjust the moisture content of the lees to 40%, add EM bacterial agent at a rate of 0.75% by mass of the lees, mix well, and ferment for 10 days while maintaining the temperature at 40° C., wherein the EM bacterial agent includes yeast, photosynthetic bacteria, lactic acid bacteria, and actinomycetes at a viable cell count ratio of 1:1:1:1.

[0101] First, the wine lees are sterilized and insect eggs are killed to provide a sterile environment for subsequent fermentation to improve the fermentation effect. Then the humidity of the wine lees is adjusted to be suitable for fermentation. The use of EM bacteria can promote the full fermentation and decomposition of the wine lees.

[0102] Preferably, the step of demagnetizing the ferroferric oxide particles comprises:

[0103] S11b, placing the ferroferric oxide particles in a demagnetizer and treating them at a frequency of 1.3 Hz and a magnetic field strength of 2500 Oersteds for 4 minutes;

[0104] S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

[0105] The above steps can be used to demagnetize the ferroferric oxide particles.

[0106] Preferably, the particle size of the ferrosoferric oxide particles is 0.5 mm.

[0107] Preferably, the ferroferric oxide is treated by the following steps before demagnetization:

[0108] S11c, dissolving low-density polyethylene in xylene solvent to prepare a 12 wt% low-density polyethylene solution;

[0109] S12c, preheating the ferrosoferric oxide particles to 90° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.3 m / s to disperse them;

[0110] S13c, at a fluidized bed temperature of 125°C, spray a low-density polyethylene solution at a rate of 3 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of the sprayed liquid being 10% of the mass of the iron powder;

[0111] S14c. Maintaining the inert gas flow rate, lower the fluidized bed temperature to below 25°C at 5°C / min, and then keep the ferroferric oxide particles dispersed by air jet in the fluidized bed for 35 minutes. Pass the wrapped ferroferric oxide particles through a 20-mesh sieve to obtain single ferroferric oxide particles wrapped with a plastic anti-rust layer. Optionally, ferroferric oxide particles that are wrapped uniformly without cracks can be manually screened out for backup.

[0112] The above process uses polyethylene to completely and evenly coat the outer surface of the ferroferric oxide particles. This prevents further oxidation of the ferroferric oxide particles in the culture medium, ensuring stable magnetic properties after magnetization. It also prevents contamination of the culture medium caused by oxidation of the ferroferric oxide particles and facilitates particle recovery. The coated ferroferric oxide particles also release trace iron into the matrix for absorption by the seedlings, thereby promoting root development.

[0113] Preferably, the magnetization treatment method of ferroferric oxide is: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 6000 Oersteds, a pulse frequency of 4 times / s, and a duration of 75s.

[0114] The purpose of the above treatment process is to magnetize the ferrosoferric oxide particles uniformly mixed in the matrix as a whole.

[0115] Preferably, the preparation steps of the bacteria-loaded bamboo fiber include the following:

[0116] S41a, crush the bamboo fiber into 1 mm, soak it in 3.5% hydrogen peroxide for 30 minutes to remove impurities, and dry it to a moisture content of 5%.

[0117] S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, with a live bacterial concentration of 5×10 9 CFU / mL;

[0118] S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 4% of the bamboo fiber mass, fermenting at 33°C and 57% humidity for 7 days, stirring once every 24 hours;

[0119] S44a, drying at 43° C. to a moisture content of 10% to obtain bacteria-laden bamboo fiber.

[0120] The natural antibacterial properties of bamboo fiber and the biological control function of the loaded bacterial strain form a synergistic effect, significantly improving the inhibitory ability against soil-borne pathogens. At the same time, the organic acids, auxins and other substances produced by the metabolism of the bacterial strains can promote the development of the seedling root system, while the moisture absorption and air permeability of bamboo fiber provide a suitable microenvironment for the bacterial strains, thereby extending the effective period of the function.

[0121] Comparative Example 1

[0122] The difference from Example 3 is that:

[0123] Replace 0.6 parts of ferroferric oxide particles with 0.6 parts of bacteria-loaded bamboo fiber, and delete the relevant processing procedures for ferroferric oxide particles. Details are as follows:

[0124] A distiller's grains seedling matrix comprises, by weight, 50 parts of distiller's grains, 30 parts of coconut bran, 10 parts of perlite, 10 parts of humic acid and 10.6 parts of bacteria-loaded bamboo fiber, wherein the distiller's grains are white wine distiller's grains.

[0125] A preparation process of a distiller's grains seedling matrix comprises the following steps:

[0126] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0127] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 25 rpm for 20 min to form a primary mixed matrix;

[0128] S4. Add the primary mixed matrix, perlite and bacteria-loaded bamboo fiber into a three-dimensional mixer and mix at 25 rpm for 30 minutes. During the stirring process, water and limestone are added to make the water content of the mixed matrix 37% and the pH value 6.5.

[0129] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0130] S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 3.5 kGy and a process temperature of 40°C;

[0131] S12a. Adjust the moisture content of the lees to 40%, add EM bacterial agent at a rate of 0.75% by mass of the lees, mix well, and ferment for 10 days while maintaining the temperature at 40° C., wherein the EM bacterial agent includes yeast, photosynthetic bacteria, lactic acid bacteria, and actinomycetes at a viable cell count ratio of 1:1:1:1.

[0132] Preferably, the preparation steps of the bacteria-loaded bamboo fiber include the following:

[0133] S41a, crush the bamboo fiber into 1 mm, soak it in 3.5% hydrogen peroxide for 30 minutes to remove impurities, and dry it to a moisture content of 5%.

[0134] S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, with a live bacterial concentration of 5×10 9 CFU / mL;

[0135] S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 4% of the bamboo fiber mass, fermenting at 33°C and 57% humidity for 7 days, stirring once every 24 hours;

[0136] S44a, drying at 43° C. to a moisture content of 10% to obtain bacteria-laden bamboo fiber.

[0137] Comparative Example 2

[0138] The difference from Example 3 is that:

[0139] Replace 10 parts of bacteria-loaded bamboo fiber with 10 parts of ferroferric oxide particles, and delete the relevant treatment process of bacteria-loaded bamboo fiber. The details are as follows:

[0140] A distiller's grains seedling matrix comprises, by weight, 50 parts of distiller's grains, 30 parts of coconut bran, 10 parts of perlite, 10 parts of humic acid and 10.6 parts of ferroferric oxide particles, wherein the distiller's grains are white wine distiller's grains.

[0141] A preparation process of a distiller's grains seedling matrix comprises the following steps:

[0142] S1, fermenting the lees and demagnetizing the ferroferric oxide particles;

[0143] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 25 rpm for 20 min to form a primary mixed matrix;

[0144] S4. Add the primary mixed matrix, perlite, and ferrosoferric oxide particles into a three-dimensional mixer and mix at 25 rpm for 30 min. During the mixing process, water and limestone are added to adjust the water content of the mixed matrix to 37% and the pH value to 6.5.

[0145] S5. Magnetizing the ferroferric oxide particles.

[0146] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0147] S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 3.5 kGy and a process temperature of 40°C;

[0148] S12a. Adjust the moisture content of the lees to 40%, add EM bacterial agent at a rate of 0.75% by mass of the lees, mix well, and ferment for 10 days while maintaining the temperature at 40° C., wherein the EM bacterial agent includes yeast, photosynthetic bacteria, lactic acid bacteria, and actinomycetes at a viable cell count ratio of 1:1:1:1.

[0149] Preferably, the step of demagnetizing the ferroferric oxide particles comprises:

[0150] S11b, placing the ferroferric oxide particles in a demagnetizer and treating them at a frequency of 1.3 Hz and a magnetic field strength of 2500 Oersteds for 4 minutes;

[0151] S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

[0152] The above steps can be used to demagnetize the ferroferric oxide particles.

[0153] Preferably, the particle size of the ferrosoferric oxide particles is 0.5 mm.

[0154] Preferably, the ferroferric oxide is treated by the following steps before demagnetization:

[0155] S11c, dissolving low-density polyethylene in xylene solvent to prepare a 12 wt% low-density polyethylene solution;

[0156] S12c, preheating the ferrosoferric oxide particles to 90° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.3 m / s to disperse them;

[0157] S13c, at a fluidized bed temperature of 125°C, spray a low-density polyethylene solution at a rate of 3 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of the sprayed liquid being 10% of the mass of the iron powder;

[0158] S14c. Maintaining the inert gas flow rate, lower the fluidized bed temperature to below 25° C. at a rate of 5° C. / min, and then allow the ferroferric oxide particles to be dispersed by air jets in the fluidized bed for 35 minutes. Pass the coated ferroferric oxide particles through a 20-mesh sieve.

[0159] Preferably, the magnetization treatment method of ferroferric oxide is: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 6000 Oersteds, a pulse frequency of 4 times / s, and a duration of 75s.

[0160] Comparative Example 3

[0161] The difference from Example 3 is that:

[0162] Remove 0.6 parts of ferroferric oxide particles and 10 parts of bacteria-carrying bamboo fiber, and delete the relevant processing processes for ferroferric oxide particles and bacteria-carrying bamboo fiber. The details are as follows:

[0163] A distiller's grains seedling matrix comprises, by weight, 50 parts of distiller's grains, 30 parts of coconut bran, 10 parts of perlite and 10 parts of humic acid, wherein the distiller's grains are white wine distiller's grains.

[0164] A preparation process of a distiller's grains seedling matrix comprises the following steps:

[0165] S1, fermenting the vinasse;

[0166] S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 25 rpm for 20 min to form a primary mixed matrix;

[0167] S4. Add the primary mixed matrix and perlite into a three-dimensional mixer and mix at 25 rpm for 30 minutes. During the mixing process, add water and limestone to adjust the water content of the mixed matrix to 37% and the pH value to 6.5.

[0168] Preferably, the fermentation treatment of vinasse comprises the following steps:

[0169] S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 3.5 kGy and a process temperature of 40°C;

[0170] S12a. Adjust the moisture content of the lees to 40%, add EM bacterial agent at a rate of 0.75% by mass of the lees, mix well, and ferment for 10 days while maintaining the temperature at 40° C., wherein the EM bacterial agent includes yeast, photosynthetic bacteria, lactic acid bacteria, and actinomycetes at a viable cell count ratio of 1:1:1:1.

[0171] Planting test results:

[0172] Tomato seeds were planted in the culture media prepared in Examples 1, 2 and 3 and Comparative Examples 1, 2 and 3. After 15 days of planting, the height of the plants was measured and the culture media were cleared out for testing.

[0173] 1. Count the total number of lateral roots of tomatoes grown in the six groups of culture media. In order to ensure the effectiveness of the experiment, select 5 tomatoes from the same group of culture media and count the total number of lateral roots and take the average value.

[0174] 2. Also observe the growth direction of the tomato lateral roots to determine whether the lateral roots grow evenly in all directions or whether the lateral roots grow unevenly and there are blank areas. A blank area refers to a lateral root that does not extend and grow in a certain spatial area.

[0175] 3. Randomly select 5 tomatoes from the same group and measure the plant height and then take the average. The single plant measurement method is to measure the distance from the culture medium surface to the top of the highest leaf.

[0176] 4. Detect the average length of the main root and lateral roots of each group of tomatoes. First, randomly select 5 tomatoes from the same group, measure and calculate the average length of the main root and lateral root of each tomato, and then calculate the average length of the main root and lateral root of the 5 tomatoes to reflect the validity of the data.

[0177] See the following results table for details:

[0178]

[0179]

[0180] From the above test results, it can be concluded that the seedlings in Examples 1 to 3 have no root rot phenomenon, which can further prove that they have good air permeability.

[0181] From the above test results, it can be concluded that in comparative example 1, after the ferroferric oxide particles were replaced with the bacteria-loaded bamboo fiber, the lateral root growth extension was uneven and there were blank areas, and the number of lateral roots was small and slow, and the plants and main roots grew slowly. In comparative example 2, after the bacteria-loaded bamboo fiber was replaced with ferroferric oxide particles, although the uniform magnetic field could induce the lateral roots to grow evenly all around, the number of lateral growth was small and slow, and the plants and main roots grew slowly. In comparative example 3, after the ferroferric oxide particles and the bacteria-loaded bamboo fiber were removed, the lateral root growth extension was uneven and there were blank areas, and the number of lateral roots was relatively small. The amount of main root and lateral root of the seedlings prepared in the embodiment 1 to the embodiment 3 is less than that in the comparative example 1 and the comparative example 2, and the length of the lateral root and the main root is also shorter than that in the comparative example 1 and the comparative example 2, and the height of the plant is also significantly shorter than that in the comparative example 1 and the comparative example 2; compared with the embodiments 1 to 3, whether it is the growth height of the plant, the growth and extension of the lateral roots to the surrounding areas, or the growth length of the lateral roots, they are better than the comparative examples. Therefore, it can be concluded that the seedling substrate prepared by the reasonable component ratio and preparation process in the embodiments 1 to 3 can effectively promote the development and growth of the seedling root system, especially after the magnetized ferroferric oxide particles and the bacteria-loaded bamboo fiber are matched, they can cooperate with each other to induce the root development growth speed and direction to a greater extent.

[0182] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A distiller's grains seedling matrix, characterized by: The invention comprises, by weight, 40-60 parts of distiller's grains, 25-35 parts of coconut bran, 7-12 parts of perlite, 7-12 parts of humic acid, 5-15 parts of bacteria-loaded bamboo fiber and 0.4-0.8 parts of ferroferric oxide particles.

2. The vinasse seedling matrix according to claim 1, wherein: The invention comprises 50 parts of wine lees, 30 parts of coconut husks, 10 parts of perlite, 10 parts of humic acid, 10 parts of bacteria-loaded bamboo fibers and 0.6 parts of ferroferric oxide particles.

3. The vinasse seedling matrix according to claim 1, wherein: The lees are white wine lees.

4. The process for preparing a distiller's grains seedling matrix according to any one of claims 1 to 3, characterized in that: The following steps are included: S1, fermenting the lees and demagnetizing the ferroferric oxide particles; S2, put the vinasse, coconut husk and humic acid into a twin-shaft mixer and mix at 20-30 rpm for 20 min to form a primary mixed matrix; S4. Add the primary mixed matrix, perlite, bacteria-loaded bamboo fiber, and ferroferric oxide particles into a three-dimensional mixer and mix at 20-30 rpm for 30 min. During the mixing process, water and limestone are added to adjust the water content of the mixed matrix to 35-45% and the pH value to 6.0-7.

0. S5. Magnetizing the ferroferric oxide particles.

5. The process for preparing a distiller's grains seedling matrix according to claim 4, wherein: The specific steps of fermentation treatment of wine lees include: S11a, irradiating the lees with an electron beam for sterilization, with an irradiation dose of 2-5 kGy and a process temperature of ≤40°C; S12a. Adjust the moisture content of the lees to ≤40%, add EM bacterial agent at 0.5%-1% of the weight of the lees, mix well, and then ferment for 7-10 days, maintaining the temperature ≤40°C during the process.

6. The process for preparing a distiller's grains seedling matrix according to claim 4, wherein: The demagnetization treatment step of the ferroferric oxide particles includes: S11b, placing the ferroferric oxide particles in a demagnetizer, and treating them at a frequency of 0.5-2 Hz and a magnetic field strength of 2000-3000 Oersteds for 3-5 minutes; S12b. After the treatment is completed, let it stand for more than 10 minutes and use a Gaussmeter to detect the magnetism of the powder surface at multiple points. When the residual magnetic field intensity is ≤0.5mT, demagnetization is completed, otherwise jump to S11b.

7. The process for preparing a distiller's grains seedling matrix according to claim 6, wherein: The particle size of the ferrosoferric oxide particles is 0.4-0.6 mm.

8. The process for preparing a distiller's grains seedling matrix according to claim 6, wherein: The ferroferric oxide is treated by the following steps before demagnetization: S11c, dissolving low-density polyethylene in xylene solvent to prepare a 10-15 wt% low-density polyethylene solution; S12c, preheating the ferrosoferric oxide particles to 80-100° C., placing them into a fluidized bed, and introducing an inert gas at a flow rate of 1.2-1.5 m / s to disperse them; S13c, at a fluidized bed temperature of 120-130° C., spraying a low-density polyethylene solution at a rate of 2-4 mL / min per kilogram of ferrosoferric oxide particles, with the total amount of the sprayed liquid being 8-12% of the mass of the iron powder; S14c. Maintaining the inert gas flow rate, reduce the fluidized bed temperature to below 25° C. at a rate of 3-7° C. / min, and allow the ferroferric oxide particles to be dispersed by air jets in the fluidized bed for ≥30 min. Pass the coated ferroferric oxide particles through a 20-mesh sieve.

9. The process for preparing a distiller's grains seedling matrix according to claim 4, wherein: The magnetization treatment method of ferroferric oxide is as follows: placing the mixed matrix in a pulsed magnetic field with a magnetic field strength of 5000-7000 Oersteds, a pulse frequency of 3-5 times / s, and a duration of 60-90s.

10. The process for preparing a distiller's grains seedling matrix according to claim 4, characterized in that: The preparation steps of the bacteria-loaded bamboo fiber include the following: S41a. Crush the bamboo fiber into pieces of 0.8-1.2 mm, soak it in 3-4% hydrogen peroxide for 30 minutes to remove impurities, and dry it to a moisture content of ≤5%. S42a, prepare a composite bacterial solution of Bacillus subtilis and Bacillus mucilaginosus at a live bacterial count ratio of 1:1, and the live bacterial concentration of the composite bacterial solution is ≥ 5×10 9 CFU / mL; S43a, spraying the composite bacterial solution onto the bamboo fiber at a spraying amount of 3-5% of the bamboo fiber mass, fermenting at 30-35°C and 55-60% humidity for 7 days, stirring once every 24 hours; S44a, drying at 40-45° C. until the moisture content is ≤10%, to obtain bacteria-laden bamboo fiber.

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