Crude extraction process of hippocampus protein

Abstract

The present invention provides a hippocampus protein crude extraction process, and relates to the technical field of protein extraction, and the hippocampus protein crude extraction process comprises the following steps: hippocampus pretreatment: hippocampus is subjected to impurity cleaning, sterilization, drying, crushing and grinding to obtain hippocampus powder, the hippocampus powder obtained in S1 is poured into a hippocampus protein extraction device, a sodium chloride solution is poured into the hippocampus protein extraction device, and the hippocampus protein is obtained; stirring the solution mixed with the hippocampus powder through a hippocampus protein extraction device, then filtering the solution through the hippocampus protein extraction device, discarding filtrate, washing filter residues through distilled water, and repeatedly washing for multiple times to obtain the non-protein-removed hippocampus powder. The hippocampus protein is extracted through the hippocampus protein extraction device, a centrifugal machine or a stirring machine does not need to be additionally used, the hippocampus protein extraction device is convenient to use, stirring, filtering, centrifuging and other work can be directly completed through the hippocampus protein extraction device, and therefore the working efficiency of hippocampus protein extraction is improved, and the operation cost is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of protein extraction, in particular to a crude extraction process of hippocampal protein. Background Art

[0002] Seahorse is a precious traditional Chinese medicine with high medicinal value. Seahorse protein is commonly used in the biopharmaceutical industry, biopharmaceutical manufacturing, genetically engineered drugs, and vaccine production. Seahorse has a long history of medicinal use, primarily used to tonify the kidneys and enhance yang, strengthen the heart and spleen, relieve coughs and asthma, and alleviate inflammation and pain. Recent research has also shown that seahorse can promote male hormone secretion and prevent breast, colon, and esophageal cancers. Seahorse has high nutritional value, with a protein content of up to 70% and seven essential amino acids. It also contains a large amount of DHA, a major building block for the prostate and sperm. Seahorse is rich in phospholipids, which are associated with its tonic effects. Its high Mn and Zn content is consistent with its kidney-tonifying and yang-enhancing effects. It is also rich in Fe, which is related to its liver and kidney-tonifying, blood-enhancing, and sperm-promoting effects. However, seahorse is currently often used as a medicinal herb infused into wine, which prevents its active ingredients from being effectively utilized. Furthermore, soaking in wine not only takes a long time but also significantly reduces its medicinal efficacy. The protein within the seahorse is often not effectively extracted, thus limiting its utilization.

[0003] To this end, the present invention provides a hippocampus protein crude extraction process. Summary of the Invention

[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a process for crude extraction of hippocampal protein to solve the problems raised in the above-mentioned background technology. The present invention does not require the use of a centrifuge or a mixer, and is relatively convenient to use. The hippocampal protein extraction device can directly complete stirring, filtering, centrifugation and other tasks, thereby improving the efficiency of extracting hippocampal protein and reducing operating costs; it can perform multiple tasks, expand the scope of application of the device, and improve the efficiency of crude extraction of hippocampal protein. The processes such as centrifugation, stirring and filtration do not affect each other, thereby ensuring the centrifugal effect, the filtering effect and the stirring effect, thereby ensuring the success rate of crude extraction of hippocampal protein; the solution can be added through the feeding structure, thereby facilitating the crude extraction of hippocampal protein, and the blocking plate can block the inner barrel during centrifugation, thereby preventing the solution in the inner barrel from spilling out; the inner wall of the inner barrel can be cleaned, and the water flow can be controlled to remain in the stirring structure according to the position of the stirring structure, and the temperature can be controlled according to the temperature of the added water, thereby facilitating the crude extraction of hippocampal protein and ensuring the success rate of extraction.

[0005] In order to achieve the above object, the present invention is implemented through the following technical solution: a hippocampus protein crude extraction process, comprising the following steps:

[0006] S1. Pretreatment of seahorses: cleaning impurities, sterilizing, drying, crushing, and grinding the seahorses to obtain seahorse powder;

[0007] S2, the hippocampus powder obtained in S1 is poured into a hippocampus protein extraction device, sodium chloride solution is poured into the hippocampus protein extraction device, the solution mixed with the hippocampus powder is stirred by the hippocampus protein extraction device, and then the solution is filtered by the hippocampus protein extraction device, the filtrate is discarded, and the filter residue is washed with distilled water, and the non-protein hippocampus powder is obtained after repeated washing.

[0008] S3, adding distilled water and isopropyl alcohol to the non-protein-removed hippocampus powder obtained in S2, and defatting the hippocampus powder using a hippocampus protein extraction device;

[0009] S4, adding distilled water to the hippocampus protein extraction device, cooling to a certain temperature after heat treatment, and adding enzyme for enzymolysis;

[0010] S5. Centrifuging the enzymatically hydrolyzed solution using a hippocampus protein extraction device, and neutralizing the upper layer of the solution to obtain crudely extracted hippocampus protein;

[0011] The hippocampus protein extraction device in S2 includes an outer shell, an outer barrel is rotatably fitted in the outer barrel, an inner barrel is slidably fitted in the outer barrel, a driving structure is fixed in the outer shell, an output end of the driving structure is equipped with a first elastic telescopic rod, an end of the first elastic telescopic rod is fixed with a first clamping block, the first clamping block corresponds to the outer barrel and the inner barrel, a clamping connection structure is installed between the first clamping block and the inner barrel, the bottom of the outer barrel and the bottom of the inner barrel are both equipped with a filtering and drainage structure, a fixing frame is fixed on the top of the inner barrel, a stirring structure is installed in the fixing frame, the stirring structure corresponds to the first clamping block, a top cover is installed on the outer shell, an electric cylinder is fixed on the top cover, a connecting rod is installed between the output end of the electric cylinder and the stirring structure, a water supply structure is installed in the stirring structure, the water supply structure corresponds to the connecting rod, a blocking plate is elastically fitted on the lower side of the top cover, a feeding structure is installed on the blocking plate, and the feeding structure corresponds to the top cover.

[0012] Furthermore, a partition is fixed inside the shell, and the driving structure includes a motor, which is located on the lower side of the partition. A discharge pipe is fixed on one side of the shell, which is located between the partition and the outer barrel. The discharge pipe is connected to the shell, and the first elastic telescopic rod is fixedly connected to the output end of the motor, and the first elastic telescopic rod is slidably connected to the partition.

[0013] Furthermore, the first elastic telescopic rod includes a first rod body and a second rod body, the first rod body is fixedly connected to the output end of the motor, the second rod body is slidably connected to the partition, the second rod body is fixedly connected to the first block, a first sliding groove is opened in the first rod body, the first sliding groove is slidably connected to the second rod body, and a first spring is fixed between the second rod body and the groove wall of the first sliding groove.

[0014] Furthermore, a first through groove is provided at the bottom of the outer barrel, and a first card slot is provided at the bottom of the inner barrel, the first card slot corresponds to the stirring structure, the first through groove and the first card slot both correspond to the first card block, and the card connection structure includes a plurality of second card blocks fixed in the first card slot, the second card block is made of elastic material, a plurality of second card slots are provided on the peripheral side of the first card block, the second card slots correspond to the second card block, a third card slot is provided on the top of the first card block, the third card slot corresponds to the stirring structure, and the first card block is a square structure.

[0015] Furthermore, the filtering and drainage structure includes a plurality of second through grooves opened at the bottom of the outer barrel, a plurality of blocking blocks are fixed at the bottom of the outer barrel, a plurality of third through grooves are opened at the bottom of the inner barrel, the blocking blocks correspond to the third through grooves, a filter screen is installed at the bottom of the inner barrel, and a pressure ring is fitted with a threaded bottom portion of the inner barrel, and the pressure ring is located above the filter screen.

[0016] Furthermore, a fourth through slot is provided in the fixing frame, and the stirring structure includes a pressure rod, which corresponds to the fourth through slot. A baffle is fixed on the pressure rod, and the baffle is located on the lower side of the fixing frame. Multiple stirring rods are fixed on the surrounding side of the baffle. A water chamber is provided in the pressure rod, and the pressure rod is rotatably connected to the connecting rod. A nut is installed between the connecting rod and the output end of the electric cylinder, and the nut is threadedly engaged with the connecting rod and the output end of the electric cylinder.

[0017] Furthermore, a first water spray head is installed at the end of the pressure rod, and multiple second water spray heads are fixed on the circumferential side of the pressure rod. The water cavity is connected to the first water spray head and the second water spray head. A slide is slidably fitted in the water cavity. The slide is fixedly connected to the first water spray head, and the slide corresponds to the second water spray head. Multiple second springs are fixed between the slide and the inner wall of the water cavity.

[0018] Furthermore, the water supply structure includes a water pipe, which is slidably connected to the top cover. One end of the water pipe is located in the water cavity. A first tube body and a second tube body are installed in the water pipe. Both the first tube body and the second tube body are connected to the water cavity.

[0019] Furthermore, the blocking plate corresponds to the inner barrel, and a plurality of second elastic telescopic rods are fixed between the blocking plate and the top cover. The second elastic telescopic rods include a third rod body and a fourth rod body. The third rod body is fixedly connected to the top cover, and the fourth rod body is fixedly connected to the blocking plate. A second sliding groove is provided in the third rod body, and the second sliding groove is slidably connected to the fourth rod body. A third spring is fixed between the fourth rod body and the second sliding groove.

[0020] Furthermore, a plurality of sliders are fixed on the inner barrel, and a plurality of third slide grooves are opened in the outer barrel, the third slide grooves correspond to the sliders, and the feeding structure includes a plurality of feeding pipes, the feeding pipes are fixedly connected to the blocking plate, the blocking plate is rotatably connected to the inner barrel, and the feeding pipes are slidably connected to the top cover.

[0021] Beneficial effects of the present invention:

[0022] 1. The hippocampus protein is extracted by the hippocampus protein extraction device without the need for a centrifuge or blender. The device is convenient to use and can directly complete stirring, filtering, centrifugation and other tasks, thereby improving the efficiency of extracting hippocampus protein and reducing operating costs.

[0023] 2. The outer barrel is rotatably installed in the outer shell, the inner barrel is slidably installed in the outer barrel, the driving structure is installed in the outer shell, and the stirring structure is installed in the fixed frame. The driving structure can drive the outer barrel and the inner barrel to rotate for centrifugation, and the stirring structure can also be used for stirring. The stirring structure drives the inner barrel to move upward for filtering, so that multiple tasks can be performed, the application scope of the device is expanded, and the efficiency of crude extraction of hippocampal protein is improved. The processes of centrifugation, stirring and filtration do not affect each other, thereby ensuring the centrifugal effect, the filtration effect and the stirring effect, thereby ensuring the success rate of crude extraction of hippocampal protein.

[0024] 3. A blocking plate is elastically installed on the lower side of the top cover, and a feeding structure is installed on the blocking plate. The solution can be added through the feeding structure, so as to facilitate the crude extraction of hippocampal protein. The blocking plate can block the inner barrel during centrifugation to prevent the solution in the inner barrel from spilling out, and the blocking plate can move relatively with the movement of the inner barrel, so as to ensure the blocking effect of the blocking plate.

[0025] 4. A water supply structure is installed in the stirring structure. Water can be added to the stirring structure through the water supply structure to clean the inner wall of the inner barrel. The water flow can be controlled to remain in the stirring structure according to the position of the stirring structure, and the temperature can be controlled according to the temperature of the added water, thereby facilitating the crude extraction of hippocampal protein and ensuring the success rate of the extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the overall assembly three-dimensional structure of a hippocampus protein extraction device in a hippocampus protein crude extraction process of the present invention;

[0027] Figure 2 This is a schematic diagram of the overall assembly cross-sectional structure of a hippocampus protein extraction device in a hippocampus protein crude extraction process of the present invention;

[0028] Figure 3 for Figure 2 Schematic diagram at A in the middle;

[0029] Figure 4 This is an exploded view of a hippocampus protein extraction device in a hippocampus protein crude extraction process of the present invention;

[0030] Figure 5 This is a schematic diagram of the assembly cross-sectional structure of the outer shell, outer barrel, and inner barrel in a process for crude extraction of hippocampal protein according to the present invention;

[0031] Figure 6 This is a schematic diagram of the assembly cross-sectional structure of the first elastic telescopic rod in a process for crude extraction of hippocampal protein according to the present invention;

[0032] Figure 7 This is a schematic diagram of the assembly cross-sectional structure of a pressure rod in a hippocampus protein crude extraction process of the present invention;

[0033] Figure 8 This is a schematic diagram of the assembled three-dimensional structure of the outer barrel in a process for crude extraction of hippocampal protein according to the present invention;

[0034] Figure 9 This is a schematic diagram of the assembled three-dimensional structure of the blocking plate and the top cover in a process for crude extraction of hippocampal protein according to the present invention;

[0035] Figure 10 This is a schematic diagram of the three-dimensional structure of the stirring structure in the crude extraction process of hippocampal protein according to the present invention;

[0036] Figure 11 This is a schematic diagram of the three-dimensional structure of the inner barrel in a process for crude extraction of hippocampal protein according to the present invention;

[0037] Figure 12 This is a flow chart of a crude extraction process of hippocampal protein according to the present invention;

[0038] In the figure: 1. outer shell; 2. outer barrel; 3. inner barrel; 4. partition; 5. motor; 6. first elastic telescopic rod; 7. first rod body; 8. second rod body; 9. first slide; 10. first spring; 11. first clamping block; 12. second clamping block; 13. first clamping slot; 14. second clamping slot; 15. third clamping slot; 16. first through slot; 17. discharge pipe; 18. second through slot; 19. third through slot; 20. blocking block; 21. filter screen; 22. pressure ring; 23. fixing bracket; 2 4. Baffle; 25. Stirring rod; 26. Pressure rod; 27. First water spray head; 28. Second water spray head; 29. ​​Slide plate; 30. Second spring; 31. Water pipe; 32. Connecting rod; 33. Electric cylinder; 34. Second elastic telescopic rod; 35. Third rod body; 36. Fourth rod body; 37. Second chute; 38. Third spring; 39. Feed pipe; 40. Blocking plate; 41. Top cover; 42. Slider; 43. Third chute; 44. Fourth through slot; 45. Nut; 46. Water cavity. DETAILED DESCRIPTION

[0039] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0040] See also Figures 1 to 12 The present invention provides a technical solution: a crude extraction process of hippocampal protein, comprising the following steps:

[0041] S1. Pretreatment of seahorses: cleaning impurities, sterilizing, drying, crushing, and grinding the seahorses to obtain seahorse powder;

[0042] S2, the hippocampus powder obtained in S1 is poured into a hippocampus protein extraction device, and a sodium chloride solution is poured into the hippocampus protein extraction device, wherein the sodium chloride solution is a 5% sodium chloride solution with a volume of 15 times that of the hippocampus powder, and the solution is continuously stirred at 3 ° C. The solution mixed with the hippocampus powder is stirred by the hippocampus protein extraction device, and then the solution is filtered by the hippocampus protein extraction device, the filtrate is discarded, and the filter residue is washed with distilled water. After repeated washing, the non-protein-removed hippocampus powder is obtained;

[0043] S3. Add distilled water (10 times the mass of the hippocampus) and isopropyl alcohol (3-8 times the mass of the hippocampus) to the non-protein-removed hippocampus powder obtained in S2, heat at 45° C., and defat the hippocampus using a hippocampus protein extraction device.

[0044] S4, adding distilled water to the hippocampus protein extraction device, heat-treating at 60°C-90°C, then cooling to a temperature below 60°C, and adding enzyme for enzymolysis;

[0045] S5. Centrifuging the enzymatically hydrolyzed solution using a hippocampus protein extraction device, and neutralizing the upper layer of the solution to obtain crudely extracted hippocampus protein;

[0046] The hippocampus protein extraction device in S2 includes a shell 1, an outer barrel 2 is rotatably fitted in the shell 1, an inner barrel 3 is slidably fitted in the outer barrel 2, a driving structure is fixed in the shell 1, a first elastic telescopic rod 6 is installed at the output end of the driving structure, a first clamping block 11 is fixed at the end of the first elastic telescopic rod 6, the first clamping block 11 corresponds to the outer barrel 2 and the inner barrel 3, a clamping connection structure is installed between the first clamping block 11 and the inner barrel 3, and a filtering and drainage structure is installed at the bottom of the outer barrel 2 and the bottom of the inner barrel 3. A fixing frame 23 is fixed on the top of the inner barrel 3, and a stirring structure is installed in the fixing frame 23, which corresponds to the first block 11. A top cover 41 is installed on the outer shell 1, and an electric cylinder 33 is fixed on the top cover 41. A connecting rod 32 is installed between the output end of the electric cylinder 33 and the stirring structure. A water supply structure is installed in the stirring structure, and the water supply structure corresponds to the connecting rod 32. A blocking plate 40 is elastically fitted on the lower side of the top cover 41, and a feeding structure is installed on the blocking plate 40, which corresponds to the top cover 41.

[0047] In this embodiment, a partition 4 is fixed inside the outer shell 1, and the driving structure includes a motor 5, which is located on the lower side of the partition 4. A discharge pipe 17 is fixed on one side of the outer shell 1, and the discharge pipe 17 is located between the partition 4 and the outer barrel 2. The discharge pipe 17 is connected to the outer shell 1, and the first elastic telescopic rod 6 is fixedly connected to the output end of the motor 5, and the first elastic telescopic rod 6 is slidably connected to the partition 4.

[0048] Specifically, by starting the motor 5, the first elastic telescopic rod 6 can be driven to rotate by the motor 5. When the first block 11 is inserted into the first slot 13, the first elastic telescopic rod 6 drives the outer barrel 2 and the inner barrel 3 to rotate through the first block 11, thereby performing centrifugation; when the first block 11 is separated from the first slot 13, the first water spray head 27 is inserted into the third slot 15, so that the first elastic telescopic rod 6 can drive the pressure rod 26 to rotate, and stirring can be performed. At this time, the outer barrel 2 and the inner barrel 3 are not in contact with the first block 11, so the outer barrel 2 and the inner barrel 3 will not rotate, and thus will not affect the stirring, so that the stirring process and the centrifugation process are relatively independent, so that the crude extraction of hippocampal protein can be better stirred and centrifuged, ensuring the effect of stirring and centrifugation, thereby ensuring the effect of crude extraction of hippocampal protein, and ensuring the success rate of crude extraction of hippocampal protein.

[0049] The first elastic telescopic rod 6 includes a first rod body 7 and a second rod body 8. The first rod body 7 is fixedly connected to the output end of the motor 5, the second rod body 8 is slidably connected to the partition 4, and the second rod body 8 is fixedly connected to the first clamping block 11. A first sliding groove 9 is opened in the first rod body 7, and the first sliding groove 9 is slidably connected to the second rod body 8. A first spring 10 is fixed between the second rod body 8 and the groove wall of the first sliding groove 9.

[0050] Specifically, when the pressure rod 26 is pressed down, the pressure rod 26 will squeeze the first block 11 downward, so that the first block 11 is squeezed downward into the second rod body 8, so that the second rod body 8 slides in the first slide groove 9, and the overall length of the first elastic telescopic rod 6 is shortened. At this time, the first spring 10 times is compressed, so that the first block 11 slides downward, so that the first block 11 is no longer in contact with the outer barrel 2 and the inner barrel 3. At this time, starting the motor 5 will not drive the outer barrel 2 and the inner barrel 3 to rotate, so that centrifugal work will not be performed. At this time, the motor 5 drives the stirring structure to stir, so that the stirring structure can be performed, thereby improving the efficiency of solution mixing, and when controlling the temperature, stirring can ensure that the temperature is relatively uniform, thereby ensuring the success rate of crude extraction of hippocampal protein.

[0051] A first through groove 16 is provided at the bottom of the outer barrel 2, and a first card groove 13 is provided at the bottom of the inner barrel 3. The first card groove 13 corresponds to the stirring structure. The first through groove 16 and the first card groove 13 both correspond to the first card block 11. The card connection structure includes a plurality of second card blocks 12 fixed in the first card groove 13. The second card block 12 is made of elastic material. A plurality of second card grooves 14 are provided on the peripheral side of the first card block 11. The second card groove 14 corresponds to the second card block 12. A third card groove 15 is provided on the top of the first card block 11. The third card groove 15 corresponds to the stirring structure. The first card block 11 is a square structure.

[0052] Specifically, the first clamping block 11 passes through the first through groove 16 and is clamped into the first clamping groove 13, so that the outer barrel 2 and the inner barrel 3 can be driven to rotate by the first clamping block 11, and the second clamping block 12 enters the second clamping groove 14, and the second clamping block 12 and the second clamping groove 14 have a clamping relationship, so that the second clamping block 12 and the inner barrel 3 are relatively fixed at this time. When the inner barrel 3 moves upward, the inner barrel 3 will pull the first clamping block 11 within a certain distance, so that the second rod 8 slides upward, and the first spring 10 is stretched, so that the first clamping block 11 will block the first clamping groove 13 to prevent the first clamping groove 13 from leaking during filtration, and when the inner barrel 3 continues to move upward, the position of the second rod 8 is limited by the first rod 7, so that the second clamping block 12 is separated from the second clamping groove 14, and the second clamping block 12 and the second clamping groove 14 lose the clamping relationship, so that the inner barrel 3 will not be limited to continue to move upward.

[0053] The filtering and drainage structure includes a plurality of second through grooves 18 opened at the bottom of the outer barrel 2, a plurality of blocking blocks 20 are fixed to the bottom of the outer barrel 2, a plurality of third through grooves 19 are opened at the bottom of the inner barrel 3, the blocking blocks 20 correspond to the third through grooves 19, a filter screen 21 is installed at the bottom of the inner barrel 3, and a pressure ring 22 is fitted with a threaded bottom thread of the inner barrel 3, and the pressure ring 22 is located on the upper side of the filter screen 21.

[0054] Specifically, when filtering is performed, the inner barrel 3 is moved upward so that a gap is generated between the inner barrel 3 and the outer barrel 2, and the blocking block 20 is separated from the third through groove 19. At this time, the solution is filtered by the filter mesh 21 and flows down from the third through groove 19, and then flows through the second through groove 18 to the partition 4, and the solution can be discharged through the discharge pipe 17. At this time, the first blocking block 11 will block the first card groove 13 to prevent the first card groove 13 from leaking during filtration, ensuring that all the solution is discharged after being filtered by the filter mesh 21, thereby ensuring the filtering effect.

[0055] The filter 21 is squeezed and fixed by the pressure ring 22 to ensure the stability of the filter 21. When the pressure ring 22 is moved upward, the pressure ring 22 no longer squeezes the filter 21, and the filter 21 can be disassembled and replaced. All residues can also be directly removed through the filter 21, making it convenient to clean the inner barrel 3.

[0056] A fourth through slot 44 is provided in the fixing frame 23, and the stirring structure includes a pressure rod 26, which corresponds to the fourth through slot 44. A baffle 24 is fixed on the pressure rod 26, and the baffle 24 is located on the lower side of the fixing frame 23. A plurality of stirring rods 25 are fixed on the surrounding side of the baffle 24. A water chamber 46 is provided in the pressure rod 26, and the pressure rod 26 is rotatably connected to the connecting rod 32. A nut 45 is installed between the connecting rod 32 and the output end of the electric cylinder 33, and the nut 45 is threadedly engaged with the output end of the connecting rod 32 and the electric cylinder 33.

[0057] Specifically, the connecting rod 32 can be driven downward by the electric cylinder 33, thereby driving the pressure rod 26 to move downward until the pressure rod 26 moves to the bottom, so that the first water spray head 27 enters the third slot 15, and the pressure rod 26 squeezes the first block 11 downward, and the pressure rod 26 can be driven to rotate by the motor 5, thereby driving the baffle 24 to rotate, and the baffle 24 drives the stirring rod 25 to rotate, and stirring can be performed; when the electric cylinder 33 pulls the connecting rod 32 to move upward, the connecting rod 32 drives the pressure rod 26 to move upward, thereby driving the baffle 24 to move upward, and the baffle 24 pulls the inner barrel 3 upward relatively, thereby performing filtering, and the up and down movement control device of the pressure rod 26 can be used to perform filtering, stirring and centrifugation, expand the scope of application of the device, reduce the operating cost of crude extraction of hippocampal protein, and there is no mutual influence between stirring, centrifugation and filtration, thereby ensuring the working effect of stirring, centrifugation and filtration, thereby improving the success rate of crude extraction of hippocampal protein.

[0058] A first water spray head 27 is installed at the end of the pressure rod 26, and multiple second water spray heads 28 are fixed on the circumferential side of the pressure rod 26. The water chamber 46 is connected to the first water spray head 27 and the second water spray head 28. A slide plate 29 is slidably fitted in the water chamber 46. The slide plate 29 is fixedly connected to the first water spray head 27. The slide plate 29 corresponds to the second water spray head 28. Multiple second springs 30 are fixed between the slide plate 29 and the inner wall of the water chamber 46. The water supply structure includes a water pipe 31, which is slidably connected to the top cover 41. One end of the water pipe 31 is located in the water chamber 46. A first tube body and a second tube body are installed in the water pipe 31. Both the first tube body and the second tube body are connected to the water chamber 46.

[0059] Specifically, the first tube body and the second tube body can have one water inlet and one water outlet. When water is inlet to the first tube body and no water is outlet from the second tube body, water can be added to the water chamber 46. If the first water spray head 27 is not in contact with the first card block 11 at this time, the water in the water chamber 46 can be sprayed out from the first water spray head 27 and the second water spray head 28, thereby adding water to the inner barrel 3 and cleaning the inner barrel 3. When the first water spray head 27 enters the third card slot 15 on the first card block 11, the third card slot 15 will block the first water spray head 27. As the pressure rod 26 moves downward, the first water spray head 27 will drive the slide plate 29 is relatively upward, and the second spring 30 is compressed, so that the slide plate 29 blocks the second water nozzle 28. At this time, the water flowing out of the first tube body is completely retained in the water cavity 46. When hot water is introduced, the solution in the inner barrel 3 can be heated. When cold water is introduced, the solution in the inner barrel 3 can be cooled. Water can also be introduced through the first tube body while being discharged through the second tube body, thereby achieving a temperature control effect. At this time, starting the motor 5 to drive the pressure rod 26 to rotate for stirring can achieve a more accurate temperature control effect, thereby facilitating the device to extract hippocampal protein at different temperatures and ensuring the success rate of the extraction.

[0060] The blocking plate 40 corresponds to the inner barrel 3, and a plurality of second elastic telescopic rods 34 are fixed between the blocking plate 40 and the top cover 41. The second elastic telescopic rods 34 include a third rod body 35 and a fourth rod body 36. The third rod body 35 is fixedly connected to the top cover 41, and the fourth rod body 36 is fixedly connected to the blocking plate 40. A second slide groove 37 is provided in the third rod body 35, and the second slide groove 37 is slidably connected to the fourth rod body 36. A third spring 38 is fixed between the fourth rod body 36 and the second slide groove 37. A plurality of sliders 42 are fixed on the inner barrel 3, and a plurality of third slide grooves 43 are provided in the outer barrel 2. The third slide groove 43 corresponds to the sliders 42. The feeding structure includes a plurality of feeding pipes 39. The feeding pipes 39 are fixedly connected to the blocking plate 40, the blocking plate 40 is rotatably connected to the inner barrel 3, and the feeding pipes 39 are slidably connected to the top cover 41.

[0061] Specifically, sodium chloride solution, isopropyl alcohol, etc. can be added into the inner barrel 3 through the feed pipe 39 to crudely extract the hippocampal protein. When the inner barrel 3 moves upward, the slider 42 slides in the third slide groove 43. At this time, the inner barrel 3 will squeeze the baffle 40 upward, thereby squeezing the fourth rod body 36. The fourth rod body 36 slides in the second slide groove 37, and the third spring 38 is compressed, so as to ensure that the baffle 40 moves with the movement of the inner barrel 3, thereby preventing the baffle 40 from limiting the movement of the inner barrel 3. When the top cover 41 is disassembled, the inner barrel 3 can be pulled out together through the connecting rod 32, the pressure rod 26 and the baffle 24, and the nut 45 can be removed to realize the disassembly of the inner barrel 3, which is convenient for cleaning the inner barrel 3.

[0062] Workflow: clean the seahorse from impurities, sterilize, dry, crush and grind it to obtain seahorse powder, then pour the seahorse powder into the seahorse protein extraction device, pour sodium chloride solution into the seahorse protein extraction device, the sodium chloride solution is 5% sodium chloride solution with a volume of 15 times that of the seahorse powder, and stir continuously at 3°C. The connecting rod 32 is driven downward by the electric cylinder 33, thereby driving the pressure rod 26 to move downward until the pressure rod 26 moves to the bottom, so that the first water spray head 27 enters the third card slot 15, and the pressure rod 26 squeezes the first card block 11 downward, and starts the motor 5. The first elastic telescopic rod 6 can be driven to rotate by the motor 5. At this time, the first water spray head 27 is inserted into the third card slot 1 5, so that at this time the first elastic telescopic rod 6 can drive the pressure rod 26 to rotate, and stirring can be carried out. Then, when the electric cylinder 33 pulls the connecting rod 32 to move upward, the connecting rod 32 drives the pressure rod 26 to move upward, thereby driving the baffle 24 to move upward. The baffle 24 relatively pulls the inner barrel 3 upward, so that a gap is generated between the inner barrel 3 and the outer barrel 2, and the block 20 is separated from the third through groove 19. At this time, the solution is filtered by the filter screen 21 and flows down from the third through groove 19, and then flows through the second through groove 18 to the partition 4, and the solution can be discharged through the discharge pipe 17, and the filtrate is discarded. Then water enters the first tube body, and water can be added to the water cavity 46. At this time, the first water spray head 27 is not in contact with the first card The blocks 11 are in contact, so the water in the water cavity 46 can be sprayed out from the first water spray head 27 and the second water spray head 28, thereby adding water to the inner barrel 3. After repeated washing for many times, the seahorse powder with non-protein removed is obtained, and then distilled water 10 times the mass of the seahorse and isopropyl alcohol 3-8 times the mass of the seahorse are added, and hot water is added through the first tube body. At this time, when the first water spray head 27 enters the third card slot 15 on the first card block 11, the third card slot 15 will block the first water spray head 27. As the pressure rod 26 moves downward, the first water spray head 27 will drive the slide plate 29 relatively upward, and the second spring 30 is compressed, so that the slide plate 29 blocks the second water spray head 28, so that the water flowing in and out of the first tube body It is completely retained in the water cavity 46, so that the inner barrel 3 is heated, heated in an environment of 45°C, defatted by a hippocampus protein extraction device, and then distilled water is added to the hippocampus protein extraction device, heat treated in an environment of 60°C-90°C, and then cooled to a temperature below 60°C, and enzymes are added for enzymolysis. After the enzymolysis is completed, the pressure rod 26 is moved to the top by the electric cylinder 33. At this time, the first card block 11 passes through the first through groove 16 and is inserted into the first card slot 13, so that the outer barrel 2 and the inner barrel 3 can be driven to rotate by the first card block 11. Starting the motor 5 can drive the outer barrel 2 and the inner barrel 3 to rotate for centrifugation. After the upper solution is neutralized to neutral, the crudely extracted hippocampus protein can be obtained.

[0063] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A hippocampus protein crude extraction process, characterized in that: The following steps are involved: S1. Pretreatment of seahorses: cleaning impurities, sterilizing, drying, crushing, and grinding the seahorses to obtain seahorse powder; S2, the hippocampus powder obtained in S1 is poured into a hippocampus protein extraction device, sodium chloride solution is poured into the hippocampus protein extraction device, the solution mixed with the hippocampus powder is stirred by the hippocampus protein extraction device, and then the solution is filtered by the hippocampus protein extraction device, the filtrate is discarded, and the filter residue is washed with distilled water, and the non-protein hippocampus powder is obtained after repeated washing. S3, adding distilled water and isopropyl alcohol to the non-protein-removed hippocampus powder obtained in S2, and defatting the hippocampus by using a hippocampus protein extraction device; S4, adding distilled water to the hippocampus protein extraction device, cooling to a certain temperature after heat treatment, and adding enzyme for enzymolysis; S5. Centrifuging the enzymatically hydrolyzed solution using a hippocampus protein extraction device, and neutralizing the upper layer of the solution to obtain crudely extracted hippocampus protein; The hippocampus protein extraction device in S2 includes an outer shell, an outer barrel is rotatably fitted in the outer barrel, an inner barrel is slidably fitted in the outer barrel, a driving structure is fixed in the outer shell, an output end of the driving structure is equipped with a first elastic telescopic rod, an end of the first elastic telescopic rod is fixed with a first clamping block, the first clamping block corresponds to the outer barrel and the inner barrel, a clamping connection structure is installed between the first clamping block and the inner barrel, the bottom of the outer barrel and the bottom of the inner barrel are both equipped with a filtering and drainage structure, a fixing frame is fixed on the top of the inner barrel, a stirring structure is installed in the fixing frame, the stirring structure corresponds to the first clamping block, a top cover is installed on the outer shell, an electric cylinder is fixed on the top cover, a connecting rod is installed between the output end of the electric cylinder and the stirring structure, a water supply structure is installed in the stirring structure, the water supply structure corresponds to the connecting rod, a blocking plate is elastically fitted on the lower side of the top cover, a feeding structure is installed on the blocking plate, and the feeding structure corresponds to the top cover.

2. A hippocampus protein crude extraction process according to claim 1, characterized in that: A partition is fixed inside the shell, and the driving structure includes a motor, which is located on the lower side of the partition. A discharge pipe is fixed on one side of the shell, which is located between the partition and the outer barrel. The discharge pipe is connected to the shell, and the first elastic telescopic rod is fixedly connected to the output end of the motor, and the first elastic telescopic rod is slidably connected to the partition.

3. A hippocampus protein crude extraction process according to claim 2, characterized in that: The first elastic telescopic rod includes a first rod body and a second rod body, the first rod body is fixedly connected to the output end of the motor, the second rod body is slidably connected to the partition, the second rod body is fixedly connected to the first block, a first sliding groove is opened in the first rod body, the first sliding groove is slidably connected to the second rod body, and a first spring is fixed between the second rod body and the groove wall of the first sliding groove.

4. A hippocampus protein crude extraction process according to claim 1, characterized in that: The outer barrel is provided with a first through groove at the bottom, and the inner barrel is provided with a first card groove at the bottom, the first card groove corresponds to the stirring structure, and the first through groove and the first card groove both correspond to the first card block, and the card connection structure includes a plurality of second card blocks fixed in the first card groove, the second card block is made of elastic material, a plurality of second card grooves are provided on the circumference of the first card block, the second card grooves correspond to the second card block, a third card groove is provided on the top of the first card block, the third card groove corresponds to the stirring structure, and the first card block is a square structure.

5. A hippocampus protein crude extraction process according to claim 1, characterized in that: The filtering and drainage structure includes a plurality of second through grooves opened at the bottom of the outer barrel, a plurality of blocking blocks are fixed at the bottom of the outer barrel, a plurality of third through grooves are opened at the bottom of the inner barrel, the blocking blocks correspond to the third through grooves, a filter screen is installed at the bottom of the inner barrel, and a pressure ring is matched with the bottom thread of the inner barrel, and the pressure ring is located above the filter screen.

6. A hippocampus protein crude extraction process according to claim 1, characterized in that: A fourth through slot is provided in the fixing frame, and the stirring structure includes a pressure rod, which corresponds to the fourth through slot. A baffle is fixed on the pressure rod, and the baffle is located on the lower side of the fixing frame. Multiple stirring rods are fixed on the surrounding side of the baffle. A water chamber is provided in the pressure rod, and the pressure rod is rotatably connected to the connecting rod. A nut is installed between the connecting rod and the output end of the electric cylinder, and the nut is threadedly engaged with the connecting rod and the output end of the electric cylinder.

7. A hippocampus protein crude extraction process according to claim 6, characterized in that: A first water spray head is installed at the end of the pressure rod, and multiple second water spray heads are fixed on the circumferential side of the pressure rod. The water cavity is connected to the first water spray head and the second water spray head. A slide is slidably fitted in the water cavity. The slide is fixedly connected to the first water spray head, and the slide corresponds to the second water spray head. Multiple second springs are fixed between the slide and the inner wall of the water cavity.

8. A hippocampus protein crude extraction process according to claim 6, characterized in that: The water supply structure includes a water pipe, which is slidably connected to the top cover. One end of the water pipe is located in the water cavity. A first tube body and a second tube body are installed in the water pipe. Both the first tube body and the second tube body are connected to the water cavity.

9. A hippocampus protein crude extraction process according to claim 1, characterized in that: The blocking plate corresponds to the inner barrel, and multiple second elastic telescopic rods are fixed between the blocking plate and the top cover. The second elastic telescopic rods include a third rod body and a fourth rod body. The third rod body is fixedly connected to the top cover, and the fourth rod body is fixedly connected to the blocking plate. A second sliding groove is opened in the third rod body, and the second sliding groove is slidably connected to the fourth rod body. A third spring is fixed between the fourth rod body and the second sliding groove.

10. A hippocampus protein crude extraction process according to claim 1, characterized in that: A plurality of sliders are fixed on the inner barrel, a plurality of third slide grooves are opened in the outer barrel, the third slide grooves correspond to the sliders, the feeding structure includes a plurality of feeding pipes, the feeding pipes are fixedly connected to the blocking plate, the blocking plate is rotatably connected to the inner barrel, and the feeding pipes are slidably connected to the top cover.

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