Rapid drug sensitive device based on microscopic imaging technology

Abstract

The invention relates to the technical field of rapid drug sensitivity, in particular to a rapid drug sensitivity device based on a microscopic imaging technology. Comprising an objective table; the object carrying plate is arranged on the object carrying table, and the object carrying plate is provided with imaging equipment; the moving assembly is arranged on the carrying plate, and the 96-well plate needing to be observed and recorded is driven by the moving assembly to move on the upper side of the carrying plate, so that the imaging equipment can conveniently observe and record bacteria. The invention has the following effects: the scanning efficiency is improved: the design of scanning a plurality of holes at one time is adopted, and the operation frequency is greatly reduced, so that the observation efficiency is obviously improved, and automatic loading and unloading are realized: a conveying technology is introduced, automatic loading and unloading of the 96-hole plate are realized, shaking and errors generated in a manual operation process are avoided, and experimental errors are reduced; the whole process from loading, scanning and recording of the 96-well plate to data analysis does not need manual intervention, manpower resources are saved, and meanwhile the accuracy of experimental results is improved.

Description

Technical Field

[0001] The present invention relates to the field of rapid drug sensitivity technology, in particular to a rapid drug sensitivity device based on microscopic imaging technology. Background Art

[0002] During medical anti-infection treatment, in order to rationally select antimicrobial drugs, the sensitivity of bacteria at the infected site to various antibiotics should first be tested, namely the antimicrobial susceptibility test (AST). Traditional AST relies on visual observation of the test results, and it is necessary to wait for the bacteria to proliferate significantly until the culture medium changes from colorless and transparent to turbid before judging the results. Since the bacterial growth process takes time, the traditional AST method takes a long time, at least 18-24 hours.

[0003] In drug susceptibility testing, 96-well plates are a commonly used experimental tool for pre-setting antibiotics of different types and concentrations. After inoculation, bacterial growth in different wells is observed visually to assess bacterial resistance to antibiotics. However, there are the following problems when observing bacterial growth: 1. Low degree of automation: It is necessary to manually observe the bacterial growth in each well of the 96-well plate one by one, which is labor-intensive and time-consuming; 2. Poor repeatability and accuracy: Manual interpretation of results is subjective, and different people may have different judgments on the turbidity of bacterial growth, resulting in inconsistent results.

[0004] 3. Dynamic monitoring cannot be achieved: Manual reading of results can usually only be performed as a one-time endpoint judgment at the end of the experiment, and it is impossible to monitor the growth changes of bacteria at different time points in real time. Summary of the Invention

[0005] In order to solve the problems in the above background technology, the present invention provides a rapid drug sensitivity device based on microscopic imaging technology.

[0006] The technical solution of the present invention is as follows: A rapid drug sensitivity device based on microscopic imaging technology, comprising: stage; A loading plate is provided on the loading platform, and the loading plate is provided with an imaging device, and the imaging device is used to photograph and record bacteria; The moving component is arranged on the carrier plate, and the 96-well plate that needs to be observed and recorded is driven to move on the upper side of the carrier plate by the moving component, so as to facilitate the observation and recording of bacteria by the imaging device.

[0007] Preferably, the mobile assembly comprises: a first fixing frame, detachably connected to the loading plate, wherein the first fixing frame is fixedly connected to a multi-stage telescopic housing; A multi-stage electric push rod fixedly connected to the first fixing frame; A movable frame is fixedly connected to the telescopic end of the multi-stage telescopic housing and is also fixedly connected to the telescopic end of the multi-stage electric push rod; An electric turntable is provided on a side of the movable frame away from the first fixed frame, the rotating portion of the electric turntable is provided with a second fixed frame, the second fixed frame is slidably connected to symmetrically distributed sliding blocks, the sliding blocks are rotatably connected to swing frames, and pressure sensors are provided on opposite sides of the symmetrically distributed swing frames; The driving member is provided on the second fixing frame and is used for driving the symmetrically distributed sliding blocks to move toward or away from each other.

[0008] As an advantage, it also includes: An incubator covers the stage, a lifting frame is provided in the incubator, a 96-well plate for holding bacteria is placed on the lifting frame, and the sliding block is provided with an angle sensor for detecting the rotation angle of the swing frame.

[0009] As an advantage, it also includes: a connecting frame, arranged on a side of the second fixing frame close to the first fixing frame; The sliding frame is slidably connected to the connecting frame. The sliding frame is provided with symmetrically distributed sliding grooves. The swing frame slides along the adjacent sliding grooves on the sliding frame.

[0010] Preferably, the connecting frame is provided with a first oil container, the first oil container is sealingly and slidingly connected to a sliding rod fixed to the sliding frame, and the sliding rod is wound with a first spring located between the first oil container and the sliding frame.

[0011] Preferably, a sliding plate fixed to the sliding rod is sealingly and slidingly connected in the first oil container, and the sliding plate is provided with a first through hole and a second through hole. A first one-way valve is provided in the first through hole, and a second one-way valve is provided in the second through hole. The flow directions controlled by the first one-way valve and the second one-way valve in the first through hole and the second through hole are opposite.

[0012] Preferably, the sliding plate is fixedly connected to a second oil casing, and a first sliding member is sealingly and slidingly connected inside the second oil casing. The first sliding member is used to limit the first one-way valve in the first through hole, and the first oil casing is fixedly connected to a pressure relief pipe connected to the second oil casing.

[0013] Preferably, a fixed shell is fixedly connected to a side of the movable frame close to the multi-stage electric push rod, an elastic liquid bag is fixedly connected in the fixed shell, the elastic liquid bag is fixedly connected to and communicated with a liquid delivery pipe that passes through the fixed shell, the sliding rod and the sliding plate and is communicated with the second oil storage shell, the movable frame is slidably connected to an extrusion frame on a side close to the first fixed frame, the lifting frame is used to extrude the extrusion frame, a second spring is provided between the extrusion frame and the movable frame, and the extrusion frame is fixed with an extrusion rod for extruding the elastic liquid bag.

[0014] Preferably, the fixed shell is provided with an elastic telescopic pin, and a fixed cover is fixedly connected to the side of the extrusion rod close to the elastic liquid bag. The elastic telescopic pin is used to limit the position of the fixed cover. The extrusion rod is slidably connected to a second sliding member, and a third spring is provided between the second sliding member and the extrusion rod.

[0015] Preferably, the elastic telescopic pin is provided with an inclined surface on the side facing away from the elastic liquid bag, the second sliding member is provided with symmetrically distributed annular inclined surfaces, and the fixed cover is trumpet-shaped, so that the annular inclined surface of the second sliding member can fit with the inner side of the fixed cover.

[0016] The present invention aims to solve the problems of complicated operation, poor automation, time-consuming and labor-intensive operation in the current bacterial observation process, and significantly improves experimental efficiency and result reliability through the following methods: 1. Improve scanning efficiency: The design of scanning multiple holes at one time greatly reduces the number of operations, thereby significantly improving observation efficiency; 2. Automated loading and unloading: The introduction of conveying technology enables automatic loading and unloading of 96-well plates, avoiding shaking and errors caused by manual operation and ensuring bacterial stability; 3. Full process automation: From 96-well plate loading, scanning and recording to data analysis, the entire process does not require manual intervention, which not only saves human resources, but also reduces human errors and further improves the accuracy of experimental results.

[0017] This device further optimizes the experimental process, with the following specific effects: 1. Integrated design: The 96-well plate, stage and related parts are integrated into the incubator to form a closed operating environment, effectively reducing the impact of external interference on bacteria, simplifying the operating process and improving experimental efficiency; 2. Real-time observation and recording: Observation and data recording of bacterial growth in 96-well plates can be completed in the incubator without removing the 96-well plates, reducing the risk of bacterial exposure. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a top view of the three-dimensional structure of the present invention; Figure 3 is a sectional view of the three-dimensional structure of the incubator of the present invention; Figure 4 Schematic diagram of the three-dimensional structure of the loading platform and its auxiliary parts of the present invention; Figure 5 Schematic diagram of the three-dimensional structure of the mobile assembly of the present invention; Figure 6 It is a three-dimensional structural diagram of the positional relationship between the sliding block and the swing frame of the present invention; Figure 7 A bottom view of the three-dimensional structure of the mobile assembly of the present invention; Figure 8 A top view of the three-dimensional structure of the sliding block and the swing frame of the present invention; Figure 9 A sectional view of the three-dimensional structure of the sliding block of the present invention; Figure 10 This is a sectional view of the three-dimensional structure of the first oil container of the present invention; Figure 11 A three-dimensional structural cross-sectional view of the sliding plate of the present invention; Figure 12 It is a three-dimensional structural diagram of the position relationship of the extrusion frame of the present invention; Figure 13 This is a sectional view of the three-dimensional structure of the fixed shell of the present invention; Figure 14 A three-dimensional structural cross-sectional view of the extrusion rod of the present invention; Figure 15 This is a schematic diagram of the three-dimensional structure of the 96-well plate of the present invention when it is offset.

[0019] Explanation of the reference numerals: 1-incubator, 2-lifting frame, 3-loading platform, 4-loading plate, 5-imaging device, 6-first fixed frame, 7-multi-stage telescopic shell, 8-multi-stage electric push rod, 9-moving frame, 10-electric turntable, 11-second fixed frame, 12-sliding block, 13-swinging frame, 14-driving member, 15-connecting frame, 16-sliding frame, 17-first oil container, 18-sliding rod, 19-sliding plate, 20-first through hole, 21-second through hole, 22-second oil container, 23-first sliding member, 24-fixed shell, 25-elastic liquid sac, 26-liquid delivery pipe, 27-extrusion frame, 28-extrusion rod, 29-elastic telescopic pin, 30-fixed cover, 31-second sliding member. DETAILED DESCRIPTION

[0020] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0021] Example 1: A rapid drug sensitivity device based on microscopic imaging technology, such as Figure 1-Figure 4 As shown, it includes: a stage 3; a stage 4, which is arranged on the stage 3, and the stage 4 is provided with an imaging device 5, which is used to photograph and record bacteria; a moving component, which is arranged on the stage 4, and the 96-well plate that needs to be observed and recorded is driven by the moving component to move on the upper side of the stage 4, so that the imaging device 5 can observe and record the bacteria.

[0022] In the above scheme, the problem of low efficiency, poor stability and waste of resources when using existing microscopes to observe the bacterial reproduction in 96-well plates is solved. The moving component actively drives the 96-well plate to move slowly, so that the imaging device 5 observes and records a row of the 96-well plate at one time, thereby shortening the overall observation and recording time. The imaging device 5 is composed of eight lenses and a camera device, which is used to take pictures of eight wells at the same time. The imaging device 5 uses phase contrast microscopy technology and observes the growth of bacteria in the 96-well plate from the bottom. The right side of the stage 3 is the control terminal, and all electrical components in the device are electrically connected to the control terminal.

[0023] like Figure 4-Figure 8 As shown, the moving assembly includes: a first fixed frame 6, which is detachably connected to the loading plate 4, and the first fixed frame 6 is fixedly connected to a multi-stage telescopic housing 7; a multi-stage electric push rod 8, which is fixedly connected to the first fixed frame 6, and the telescopic portion of the multi-stage electric push rod 8 passes through the first fixed frame 6; a moving frame 9, which is fixedly connected to the telescopic end of the multi-stage telescopic housing 7 and is fixedly connected to the telescopic tube of the multi-stage electric push rod 8; an electric turntable 10, which is arranged on the side of the moving frame 9 away from the first fixed frame 6, and the rotating portion of the electric turntable 10 is provided with a second fixed frame 11, and the second fixed frame 11 is slidably connected to symmetrically distributed sliding blocks 12, and the sliding blocks 12 are rotatably connected to the swing frames 13, and the opposite sides of the symmetrically distributed swing frames 13 are provided with pressure sensors; a driving member 14, which is arranged on the second fixed frame 11, and is used to drive the symmetrically distributed sliding blocks 12 to move toward or away from each other, and the driving member 14 is a double-axis head motor, and the output shaft of the driving member 14 is fixedly connected to a threaded rod threadedly connected to the adjacent sliding blocks 12, and the rotation directions of the two threaded rods are opposite.

[0024] In the above scheme, the first fixed frame 6 is located on the right side of the loading plate 4, and the two are bolted together. The telescopic end of the multi-stage telescopic shell 7 is bolted to the mobile frame 9, and the fixed part of the multi-stage telescopic shell 7 is fixed to the first fixed frame 6. The multi-stage telescopic shell 7 is used to enhance the stability of the mobile frame 9 during movement. The length of the telescopic part of the multi-stage electric push rod 8 after full extension is greater than the length of the loading plate 4. The electric turntable 10 is composed of a rotating part, a base and a power part. The power part is used to drive the rotating part to rotate on the base. The base of the electric turntable 10 is bolted to the left side of the mobile frame 9, and the rotating part of the electric turntable 10 is bolted to the second fixed frame 11. The electric turntable 10 is used to adjust the rotation angle of the second fixed frame 11. The pressure sensors on the opposite sides of the two symmetrically distributed swing frames 13 are used to monitor in real time whether the swing frames 13 are in contact with the 96-well plate.

[0025] Working principle: When it is necessary to observe and record the bacteria in the 96-well plate, the user controls the telescopic part of the multi-stage electric push rod 8 to slowly extend through the control terminal. During the extension process, the telescopic part of the multi-stage electric push rod 8 drives the movable frame 9 and the parts thereon to move synchronously (the telescopic part of the multi-stage telescopic cover 7 is stretched during the movement of the movable frame 9).

[0026] When the movable frame 9 drives the second fixed frame 11 to move above the 96-well plate, the telescopic part of the multi-stage electric push rod 8 stops moving, and then the control terminal starts the driving member 14, which drives the two sliding blocks 12 to move toward each other. During the movement of the sliding block 12, it drives the swing frame 13 on it to move toward the direction of the electric turntable 10.

[0027] When the facing sides of the two swing frames 13 are in contact with the 96-well plate, the pressure sensors of the two swing frames 13 are in contact with the 96-well plate, and then the signals are transmitted to the control terminal through the pressure sensors on the two swing frames 13, and the control terminal turns off the driving member 14, thereby pausing the movement of the two sliding blocks 12 and the swing frames 13. At this time, the two swing frames 13 have grasped the 96-well plate, and then the telescopic part of the multi-stage electric push rod 8 is controlled to retract through the control terminal. During the retraction of the telescopic part of the multi-stage electric push rod 8, the mobile frame 9 and the 96-well plate grasped by the two swing frames 13 are driven to move slowly to the right at the same time (in this process, the telescopic part of the multi-stage telescopic cover 7 is retracted).

[0028] When the 96-well plate captured by the movable rack 9 and the two swing racks 13 moves to the upper side of the sample plate 4, the telescopic part of the multi-stage electric push rod 8 moves intermittently, so that each row of bacteria on the 96-well plate can be recorded by the imaging device 5. After the telescopic part of the multi-stage electric push rod 8 moves until all the bacteria on the 96-well plate are recorded, the control terminal controls the telescopic part of the multi-stage electric push rod 8 to extend.

[0029] The above-mentioned action can be repeated during the extension of the telescopic part of the multi-stage electric push rod 8. When the extension distance of the telescopic part of the multi-stage electric push rod 8 reaches the position where the 96-well plate is placed, the control terminal starts the driving member 14, so that the driving member 14 drives the two sliding blocks 12 to move back to back. During the movement of the sliding block 12, the swing frame 13 is driven to move synchronously to release the grip of the 96-well plate. The two sliding blocks 12 move to Figure 7 After reaching the basic state, the driving member 14 is closed, and then the telescopic portion of the multi-stage electric push rod 8 is retracted by the control terminal, and the process of retracting the telescopic portion of the multi-stage electric push rod 8 is repeated as described above.

[0030] When you need to observe and record the bacteria in the 96-well plate again, repeat the above steps. The above steps achieve the following results: 1. Scan eight holes at a time, significantly reducing the number of manual operations and greatly improving observation and recording efficiency; 2. The conveying technology is used to realize automatic loading and unloading of 96-well plates, avoiding shaking and errors caused by manual operation, ensuring bacterial stability and experimental reliability.

[0031] Example 2: Based on Example 1, Figure 1-Figure 4 、 Figure 9 、 Figure 14 and Figure 15 As shown, it also includes: an incubator 1, the incubator 1 covers the stage 3, and transparent glass is provided on the front and right side of the incubator 1 for convenient observation of the situation inside the incubator 1. A lifting frame 2 is provided in the incubator 1, and a 96-well plate for holding bacteria is placed on the lifting frame 2. The sliding block 12 is provided with an angle sensor for detecting the rotation angle of the swing frame 13.

[0032] In the above scheme, in order to facilitate the observation and recording of bacteria in the 96-well plate, the stage 3 is placed in the incubator 1 to facilitate the taking and placement of the 96-well plate. When the device is in use, the incubator 1 is connected to an external heating device, and the heating temperature of the heating device can be 35° (this temperature is a suitable temperature for bacterial reproduction). The angle sensor is located on the lower side of the sliding block 12 and is connected to the adjacent swing frame 13.

[0033] like Figures 5-10 As shown, it also includes: a connecting frame 15, which is arranged on the side of the second fixed frame 11 close to the first fixed frame 6; a sliding frame 16, which is slidably connected to the connecting frame 15, and the sliding frame 16 is provided with symmetrically distributed sliding grooves, and the swing frame 13 slides along the adjacent sliding grooves on the sliding frame 16.

[0034] In the above scheme, the connecting frame 15 is bolted to the second fixed frame 11, the center line of the sliding frame 16 is parallel to the center line of the second fixed frame 11, the sliding frames 16 are distributed in a cross shape with the mobile frame 9, and the projections of the right parts of the two swing frames 13, the sliding frames 16 and the second fixed frame 11 on the horizontal plane are rectangular.

[0035] like Figure 7 and Figure 10 As shown, the connecting frame 15 is provided with a first oil container 17 , and the first oil container 17 is sealingly and slidingly connected to a sliding rod 18 fixed to the sliding frame 16 , and the sliding rod 18 is wound with a first spring located between the first oil container 17 and the sliding frame 16 .

[0036] In the above solution, the first oil container 17 is filled with hydraulic oil, and the spring wound on the sliding rod 18 is only used to drive the sliding frame 16 to slide and reset.

[0037] like Figure 7 、 Figure 10 and Figure 11 As shown, a sliding plate 19 fixed to the sliding rod 18 is sealed and slidably connected in the first oil container 17, and the sliding plate 19 is provided with a first through hole 20 and a second through hole 21. A first one-way valve is provided in the first through hole 20, and a second one-way valve is provided in the second through hole 21. The flow directions controlled by the first one-way valve and the second one-way valve in the first through hole 20 and the second through hole 21 are opposite.

[0038] In the above scheme, the sliding plate 19 is initially located in the right part of the first oil container 17. When the sliding plate 19 slides to the left, the second one-way valve in the second through hole 21 is in an open state. When the sliding plate 19 slides to the right, the first one-way valve in the first through hole 20 is in an open state.

[0039] like Figure 8 and Figure 10 As shown, the sliding plate 19 is fixedly connected to the second oil containing shell 22, and the first sliding member 23 is sealed and slidably connected inside the second oil containing shell 22. The first sliding member 23 is used to limit the first one-way valve in the first through hole 20, and the first oil containing shell 17 is fixedly connected to a pressure relief pipe connected to the second oil containing shell 22.

[0040] In the above scheme, when the first sliding member 23 limits the first one-way valve in the first through hole 20, the sliding plate 19 can only slide to the left along the first oil casing 17, and the pressure relief pipe on the left side of the second oil casing 22 is connected to the outside world, which is used to discharge the pressure in the lower part of the second oil casing 22. When the sliding plate 19 slides to the left along the first oil casing 17, the pressure relief pipe on the left side of the second oil casing 22 becomes loose.

[0041] like Figure 12 and Figure 13As shown, a fixed shell 24 is fixedly connected to the side of the movable frame 9 close to the multi-stage electric push rod 8, and an elastic liquid capsule 25 is fixedly connected in the fixed shell 24. The elastic liquid capsule 25 is fixedly connected and communicated with a liquid delivery pipe 26 that passes through the fixed shell 24, the sliding rod 18 and the sliding plate 19 and is communicated with the second oil container 22. An extrusion frame 27 is slidably connected to the side of the movable frame 9 close to the first fixed frame 6. The lifting frame 2 is used to extrude the extrusion frame 27. A second spring is provided between the extrusion frame 27 and the movable frame 9. The extrusion frame 27 is fixed with an extrusion rod 28 for extruding the elastic liquid capsule 25.

[0042] In the above scheme, the elastic liquid sac 25 is not initially in contact with the right side surface inside the fixed shell 24, which is used to give the elastic liquid sac 25 a margin for being squeezed. The second spring between the squeezing frame 27 and the movable frame 9 is used to drive the squeezing frame 27 to reset. Hydraulic oil is stored in the elastic liquid sac 25, the liquid feeding pipe 26, and the upper part of the second oil storage shell 22. When the elastic liquid sac 25 is squeezed, the hydraulic oil in the elastic liquid sac 25 enters the second oil storage shell 22 through the liquid feeding pipe 26 and pushes the first sliding member 23 downward.

[0043] like Figure 13 and Figure 14 As shown, the fixed shell 24 is provided with an elastic telescopic pin 29, and a fixed cover 30 is fixedly connected to the side of the extrusion rod 28 close to the elastic liquid sac 25. The elastic telescopic pin 29 is used to limit the position of the fixed cover 30. The extrusion rod 28 is slidably connected to the second sliding member 31, and a third spring is provided between the second sliding member 31 and the extrusion rod 28. The elastic telescopic pin 29 is provided with an inclined surface on the side facing away from the elastic liquid sac 25, and the second sliding member 31 is provided with symmetrically distributed annular inclined surfaces. The fixed cover 30 is trumpet-shaped, which is used to enable the annular inclined surface of the second sliding member 31 to fit with the inner side of the fixed cover 30.

[0044] In the above scheme, when the fixed cover 30 and the second sliding member 31 approach the direction of the elastic liquid bag 25, the right side of the two can squeeze the elastic telescopic pin 29 upward, and the third spring on the second sliding member 31 is used to drive the second sliding member 31 to reset. The elastic coefficient of the third spring on the second sliding member 31 is smaller than the elastic coefficient of the elastic telescopic pin 29.

[0045] Working principle: Before observing and recording the bacteria in the 96-well plate, the user first connects the incubator 1 to the external heating device, then opens the incubator 1. The user first places multiple 96-well plates containing bacteria on the lifting rack 2 in sequence, then closes the incubator 1 and turns on the external heating device at the same time. After the temperature in the incubator 1 gradually rises to a temperature suitable for bacteria, the external heating device keeps the temperature in the incubator 1 warm.

[0046] When it is necessary to observe and record the bacteria in the 96-well plate, the staff first controls the lifting frame 2 through the control terminal to lift the 96-well plate on it to a height suitable for subsequent grasping, and then controls the telescopic end of the multi-stage electric push rod 8 to extend. The process of extending the telescopic end of the multi-stage electric push rod 8 can be repeated as described above. When the mobile frame 9 is pushed by the telescopic end of the multi-stage electric push rod 8 to the point where the extrusion frame 27 contacts the lifting frame 2, the telescopic end of the multi-stage electric push rod 8 continues to push the mobile frame 9 to the left. At this time, the extrusion frame 27 is relatively displaced with the mobile frame 9 (the extrusion frame 27 and the lifting frame 2 remain stationary, but the second spring on the extrusion frame 27 is squeezed), causing the extrusion rod 28, the fixed cover 30 and the second sliding member 31 to also be relatively displaced with the fixed shell 24.

[0047] When the fixed cover 30 approaches the elastic liquid sac 25, it first squeezes the inclined surface of the elastic telescopic pin 29 (when the inclined surface of the elastic telescopic pin 29 is squeezed, it moves upward and is in a compressed state). As the distance between the fixed cover 30 and the elastic liquid sac 25 is shortened, the fixed cover 30 squeezes the elastic telescopic pin 29 upward until the right side of the fixed cover 30 is no longer in contact with the inclined surface of the elastic telescopic pin 29 (the elastic telescopic pin 29 re-extends under the action of its own elastic force), and the left side of the fixed cover 30 slides to the right side of the elastic telescopic pin 29. Subsequently, the distance between the fixed cover 30 and the elastic liquid sac 25 continues to shorten until the elastic liquid sac 25 is squeezed by the squeezing rod 28. After the elastic liquid sac 25 is squeezed, the hydraulic oil therein enters the second oil storage shell 22 through the liquid feeding pipe 26, increasing the pressure in the second oil storage shell 22 to push the first sliding member 23 downward, and the first sliding member 23 moves downward. During the movement, the gas in the lower part of the second oil storage shell 22 is first emptied (the gas in the lower part of the second oil storage shell 22 is discharged from the pressure relief pipe as the first sliding member 23 moves). During the movement of the first sliding member 23, the first one-way valve in the first through hole 20 is restricted, limiting the opening of the first through hole 20, so that the sliding plate 19 can only move in one direction to the left in the first oil storage shell 17. At this time, the fixed cover 30 is located on the right side of the elastic telescopic pin 29, but the elastic telescopic pin 29 does not contact the second sliding member 31, the telescopic part of the multi-stage electric push rod 8 no longer extends, the electric turntable 10 and the parts thereon are already above the 96-hole plate in the lifting frame 2, and then the driving member 14 drives the two sliding blocks 12 to move back to back, and the two sliding blocks 12 then drive the swing frame 13 thereon to move synchronously (the swing frame 13 moves along the adjacent slide groove on the sliding frame 16 during the movement).

[0048] When the 96-well plate in the lifting frame 2 is not aligned and both swing frames 13 are in contact with the 96-well plate (i.e. Figure 15), when the 96-well plate is tilted, the two swing frames 13 squeeze the 96-well plate, and the 96-well plate generates a reaction force on the two swing frames 13, causing the two swing frames 13 to rotate along the adjacent sliding blocks 12 respectively (when the swing frames 13 rotate along the adjacent sliding blocks 12, the angle sensor on the sliding block 12 detects the rotation angle and direction of the swing frames 13). When the two swing frames 13 rotate, they drive the sliding frame 16 to move to the left and slide relative to each other, so that the projections of the right parts of the two swing frames 13, the second fixed frame 11 and the sliding frame 16 on the horizontal plane appear in a "parallelogram" state.

[0049] During the movement of the sliding frame 16, the sliding rod 18 and the sliding plate 19 are driven to move along the first oil housing 17 (the first spring is squeezed during the movement of the sliding frame 16, causing the first spring to be compressed). When the sliding plate 19 moves to the left along the first oil housing 17, the one-way valve in the second through hole 21 is opened, thereby allowing the sliding plate 19 to slide to the left in the first oil housing 17. When the opposite sides of the two swing frames 13 are in contact with the 96-hole plate, the pressure sensors on the two swing frames 13 are in contact with the 96-hole plate. At this time, the driving member 14 stops rotating, thereby completing the grabbing of the 96-hole plate (at this time, the sliding plate 19 no longer moves in the first oil housing 17, and the one-way valve in the second through hole 21 remains closed).

[0050] After completing the grabbing of the 96-well plate, the lifting frame 2 actively moves downward so that the 96-well plate is no longer in contact with the lifting frame 2. Subsequently, the telescopic part of the multi-stage electric push rod 8 drives the movable frame 9 to move to the right. During the movement of the movable frame 9 to the right, the squeezing frame 27 produces a relative displacement with the movable frame 9 under the action of the second spring (at this time, the distance between the squeezing frame 27 and the right side of the movable frame 9 gradually increases).

[0051] In the process of moving away from the moving frame 9, the extrusion frame 27 drives the fixed cover 30 to move synchronously. After the fixed cover 30 moves to its left side and is blocked by the elastic telescopic pin 29, the fixed cover 30, the extrusion rod 28 and the extrusion frame 27 stop moving. When the moving frame 9 and the grabbed 96-well plate move to the right side of the lifting frame 2, the angle sensor on the sliding block 12 has detected the rotation angle and direction of the swing frame 13. Therefore, the angle sensor on the sliding block 12 transmits an electrical signal to the control terminal, and the control terminal controls the electric turntable 10 to rotate, so that the rotating part of the electric turntable 10 drives the second fixed frame 11, the connecting frame 15, the sliding frame 16 and the grabbed 96-well plate. The well plate rotates, and during the rotation of the 96-well plate, its center line gradually coincides with the center line of the movable rack 9. At this time, the rotating part of the electric turntable 10 stops rotating, and the center line of the 96-well plate has coincided with the center line of the movable rack 9 (the effect achieved by the above action is: when the 96-well plate is offset during placement, the rotation angle of the subsequent grabbing of the 96-well plate parts is adjusted according to the offset angle of the 96-well plate, and finally the center line of the 96-well plate is coincided with the center line of the movable rack 9, thereby completing the deviation correction of the 96-well plate), the multi-stage electric push rod 8 continues to drive the 96-well plate grabbed by the movable rack 9 and its parts to continue to move to the right, thereby completing the subsequent observation and recording, and the subsequent actions are all consistent with the above.

[0052] After completing the observation and recording of the 96-well plate, the multi-stage electric push rod 8 moves the mobile rack 9 and the 96-well plate grasped by its parts to the left again. When the mobile rack 9 drives the squeezing rack 27 to contact the incubator 1 again, the mobile rack 9 has carried the 96-well plate grasped by its parts into the lifting rack 2. When the mobile rack 9 continues to move to the left, the squeezing rack 27 is blocked by the incubator 1 and moves relative to the mobile rack 9. At that time, the squeezing rod 28 drives the second sliding member 31 and the fixed cover 30 to approach the elastic liquid sac 25. When the squeezing rod 28 moves to the right, it will continue to squeeze the elastic liquid sac 25, but the liquid transported by the elastic liquid sac 25 has caused the first sliding member 23 to move and completely block the first one-way valve in the first through hole 20. Therefore, the elastic liquid sac 25 will diffuse to the surrounding when it is squeezed again.

[0053] As the extrusion rod 28 moves, the extrusion rod 28 drives the second sliding member 31 to contact the elastic telescopic pin 29, and the annular inclined surface on the right side of the second sliding member 31 contacts the inclined surface of the elastic telescopic pin 29 and squeezes it upward. When the extrusion rod 28 drives the second sliding member 31 to move until the annular inclined surface on the left side of the second sliding member 31 contacts the elastic telescopic pin 29 (the elastic telescopic pin 29 rebounds downward), the telescopic end of the multi-stage electric push rod 8 stops extending (and at this time the extension distance of the multi-stage electric push rod 8 has exceeded the previous extension distance, the purpose of which is to enable the annular inclined surface on the left side of the second sliding member 31 to contact the right side of the elastic telescopic pin 29).

[0054] When the telescopic part of the multi-stage electric push rod 8 stops extending, the driving member 14 is started to move the two swing frames 13 back to back, thereby releasing the grip of the 96-well plate (in this process, the lifting frame 2 has been lifted to a height suitable for placing the 96-well plate). The two swing frames 13 move back to Figure 6 After the state is reached, the 96-well plate is no longer grabbed. At this time, the 96-well plate has been placed on the lifting frame 2. The control terminal then turns off the driving member 14 and controls the telescopic portion of the multi-stage electric push rod 8 to reset, so that the telescopic portion of the multi-stage electric push rod 8 drives the moving frame 9 and the parts thereon to reset to the right.

[0055] During the process of the movable frame 9 returning to the right side, the extrusion frame 27 is relatively displaced with the movable frame 9 under the action of the second spring. At this time, the extrusion frame 27 drives the second sliding member 31 and the fixed cover 30 to gradually move away from the elastic liquid sac 25. Under the action of the contact between the left annular inclined surface of the second sliding member 31 and the right side of the elastic telescopic pin 29, the extrusion rod 28 and the fixed cover 30 are relatively displaced with the second sliding member 31 (at this time, the third spring on the second sliding member 31 is squeezed).

[0056] When the second sliding member 31 moves to the right annular inclined surface and fits the inner side of the fixed cover 30, the extruding rod 28, the fixed cover 30 and the second sliding member 31 continue to move away from the elastic liquid bag 25, and the annular inclined surface on the left side of the second sliding member will squeeze the elastic telescopic pin 29 upward, so that the extruding rod 28, the fixed cover 30 and the second sliding member 31 can easily move away from the elastic liquid bag 25. The extruding rod 28 no longer squeezes the elastic liquid bag 25 during the process of moving away from the elastic liquid bag 25. As the extruding rod 28 and the fixed shell 24 slide relative to each other, the extruding rod 28 drives the fixed cover 30 and the second sliding member 31 to slide until the fixed cover 30 is located on the left side of the elastic telescopic pin 29 (at which time the elastic telescopic pin 29 is no longer squeezed and the elastic telescopic pin 29 is reset by its own elastic force). After the second sliding member 31 is separated from the contact with the elastic telescopic pin 29, the second sliding member 31 is reset under the action of the third spring (see Figure 12 ).

[0057] After the elastic sac 25 is no longer squeezed, it returns to its original position by its own elastic force. During the process of returning the elastic sac 25 to its original position, the hydraulic oil in the second oil storage shell 22 is extracted through the liquid feeding pipe 26, so that the first sliding member 23 returns to its original position and slides upward, and no longer blocks the first one-way valve in the first through hole 20. Under the action of the first spring wound on the sliding rod 18, the sliding rod 18 drives the sliding frame 16 to move to the right (in the process of the two moving to the right, the one-way valve in the first through hole 20 opens, and the two swing frames 13 slide relative to the sliding frame 16). In the process of the sliding frame 16 moving to the right, the projection of the right part of the two swing frames 13, the second fixed frame 11 and the sliding frame 16 on the horizontal plane gradually changes from a "parallelogram" to a rectangle. When the above parts are transformed into a rectangle, the sliding plate 19 and the sliding rod 18 have moved to Figure 8and Figure 9 The state is then reset through the rotary portion of the electric turntable 10, which drives the second fixed frame 11, the connecting frame 15 and the sliding frame 16 to rotate and reset, so that the sliding frame 16 and the movable frame 9 maintain a cross distribution (the effect achieved by the above parts is: when grabbing a 96-well plate that is offset, the 96-well plate is first grabbed in an offset manner, and then when the 96-well plate is away from the lifting frame 2, the 96-well plate is actively driven to correct the deviation. After completing the observation and recording of the 96-well plate, the adjusted 96-well plate is put back on the lifting frame 2 to facilitate subsequent observation and recording).

[0058] When the sliding frame 16 and the moving frame 9 maintain a cross distribution, the multi-stage electric push rod 8 drives the parts thereon to move to the basic state (see Figure 5 ), when you need to observe and record the 96-well plate again, just repeat the above steps.

[0059] The embodiments of the present invention are described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Various changes can be made within the scope of knowledge of those skilled in the art without departing from the spirit of the present invention.

Claims

1. A rapid drug sensitivity device based on microscopic imaging technology, characterized in that: include: Stage (3); A loading plate (4) is arranged on the loading platform (3); the loading plate (4) is provided with an imaging device (5); the imaging device (5) is used to photograph and record bacteria; The moving component is arranged on the carrier plate (4), and the 96-well plate that needs to be observed and recorded is driven to move on the upper side of the carrier plate (4) by the moving component, so as to facilitate the observation and recording of bacteria by the imaging device (5).

2. A rapid drug sensitivity device based on microscopic imaging technology according to claim 1, characterized in that: The mobile component includes: A first fixing frame (6) is detachably connected to the loading plate (4), and the first fixing frame (6) is fixedly connected to a multi-stage telescopic housing (7); A multi-stage electric push rod (8) fixedly connected to the first fixing frame (6); A movable frame (9) is fixedly connected to the telescopic end of the multi-stage telescopic housing (7) and is also fixedly connected to the telescopic end of the multi-stage electric push rod (8); An electric turntable (10) is arranged on a side of the movable frame (9) away from the first fixed frame (6), and a second fixed frame (11) is provided on a rotating portion of the electric turntable (10), wherein the second fixed frame (11) is slidably connected to symmetrically distributed sliding blocks (12), and the sliding blocks (12) are rotatably connected to swing frames (13), and pressure sensors are provided on opposite sides of the symmetrically distributed swing frames (13); The driving member (14) is arranged on the second fixing frame (11) and is used to drive the symmetrically distributed sliding blocks (12) to move toward or away from each other.

3. A rapid drug sensitivity device based on microscopic imaging technology according to claim 2, characterized in that: Also includes: An incubator (1) covers the stage (3), a lifting frame (2) is provided in the incubator (1), a 96-well plate for holding bacteria is placed on the lifting frame (2), and the sliding block (12) is provided with an angle sensor for detecting the rotation angle of the swing frame (13).

4. A rapid drug sensitivity device based on microscopic imaging technology according to claim 2, characterized in that: Also includes: A connecting frame (15) is arranged on a side of the second fixing frame (11) close to the first fixing frame (6); The sliding frame (16) is slidably connected to the connecting frame (15), the sliding frame (16) is provided with symmetrically distributed sliding grooves, and the swing frame (13) slides along the adjacent sliding grooves on the sliding frame (16).

5. A rapid drug sensitivity device based on microscopic imaging technology according to claim 4, characterized in that: The connecting frame (15) is provided with a first oil container (17), the first oil container (17) is sealingly and slidingly connected to a sliding rod (18) fixed to the sliding frame (16), and the sliding rod (18) is wound with a first spring located between the first oil container (17) and the sliding frame (16).

6. A rapid drug sensitivity device based on microscopic imaging technology according to claim 5, characterized in that: A sliding plate (19) fixed to the sliding rod (18) is sealingly and slidingly connected in the first oil container (17), and the sliding plate (19) is provided with a first through hole (20) and a second through hole (21). A first one-way valve is provided in the first through hole (20), and a second one-way valve is provided in the second through hole (21). The flow directions controlled by the first one-way valve and the second one-way valve in the first through hole (20) and the second through hole (21) are opposite.

7. A rapid drug sensitivity device based on microscopic imaging technology according to claim 6, characterized in that: The sliding plate (19) is fixedly connected to a second oil container (22), and a first sliding member (23) is sealingly and slidingly connected inside the second oil container (22). The first sliding member (23) is used to limit the first one-way valve in the first through hole (20), and the first oil container (17) is fixedly connected to a pressure relief pipe connected to the second oil container (22).

8. The rapid drug sensitivity device based on microscopic imaging technology according to claim 7, characterized in that: The movable frame (9) is fixedly connected to a fixed shell (24) on one side close to the multi-stage electric push rod (8), and an elastic liquid bag (25) is fixedly connected inside the fixed shell (24). The elastic liquid bag (25) is fixedly connected to and communicated with a liquid delivery pipe (26) that passes through the fixed shell (24), the sliding rod (18) and the sliding plate (19) and is communicated with the second oil container (22). The movable frame (9) is slidably connected to an extrusion frame (27) on one side close to the first fixed frame (6). The lifting frame (2) is used to extrude the extrusion frame (27). A second spring is provided between the extrusion frame (27) and the movable frame (9). The extrusion frame (27) is fixedly connected to an extrusion rod (28) for extruding the elastic liquid bag (25).

9. The rapid drug sensitivity device based on microscopic imaging technology according to claim 8, characterized in that: The fixed shell (24) is provided with an elastic telescopic pin (29), and a fixed cover (30) is fixedly connected to a side of the extrusion rod (28) close to the elastic liquid bag (25). The elastic telescopic pin (29) is used to limit the position of the fixed cover (30). The extrusion rod (28) is slidably connected to a second sliding member (31), and a third spring is provided between the second sliding member (31) and the extrusion rod (28).

10. The rapid drug sensitivity device based on microscopic imaging technology according to claim 9, characterized in that: The elastic telescopic pin (29) is provided with an inclined surface on a side facing away from the elastic liquid sac (25), the second sliding member (31) is provided with symmetrically distributed annular inclined surfaces, and the fixed cover (30) is trumpet-shaped, so that the annular inclined surface of the second sliding member (31) can fit with the inner side of the fixed cover (30).

Images