A physical detection analysis device based on stem cell properties

Abstract

The application relates to the technical field of cell detection, and specifically discloses a physical detection and analysis equipment based on stem cell characteristics, which comprises a detection mechanism arranged in an analyzer, the detection mechanism comprises mounting plates, the mounting plates are arranged in an annular array, and the mounting plates are fixedly connected at one end close to each other; the top of each mounting plate is provided with a detection assembly for preparing stem cell detection liquid; and the outer side of the mounting groove is provided with a positioning assembly. The stem cell detection liquid can be prepared after the test tube is mounted in the analyzer through the detection mechanism. Compared with the traditional preparation process of the stem cell detection liquid, the test tube needs to be opened multiple times to extract supernatant or add buffer liquid, the operation is simple, the detection and analysis efficiency of the stem cells is improved, the stem cell suspension is not exposed to the external environment, the damage of the stem cells is reduced, the supernatant can be accurately extracted, and the subsequent detection and analysis is not affected.

Description

Technical Field

[0001] This invention relates to the field of cell detection technology, specifically to a physical detection and analysis device based on stem cell characteristics. Background Technology

[0002] The physical property testing of stem cells is typically conducted to ensure the quality stability and safety of stem cell preparations in clinical applications, avoiding adverse reactions caused by excessively high or low osmotic pressure. During stem cell testing and analysis, the stem cell suspension needs to be centrifuged and washed multiple times to prepare the stem cell detection solution for testing.

[0003] CN120275236B discloses a stem cell drug finished product testing device and method. The problem raised in the background technology is that existing stem cell analysis equipment is difficult to achieve full-process automated closed-loop; in particular, during centrifugation and washing, it is necessary to continuously open and close the cap to perform liquid collection and addition operations, which reduces the detection efficiency.

[0004] Based on existing technologies, the following problems exist:

[0005] In the process of stem cell detection and analysis, the test tubes need to be opened and closed repeatedly to extract supernatant, which reduces work efficiency. The aforementioned application only improves the centrifugation effect and facilitates the extraction of supernatant by keeping the test tubes vertical or tilted during opening and closing. However, it still requires opening and closing the test tube stopper multiple times, reducing detection efficiency and exposing the stem cell suspension to the external environment, which may damage the stem cells. This is not conducive to practical detection and analysis and has certain shortcomings. To solve the above problems, a physical detection and analysis device based on stem cell characteristics is proposed. Summary of the Invention

[0006] To achieve the above objectives, the present invention provides the following technical solution: a physical detection and analysis device based on stem cell characteristics, comprising an analyzer and a sampling mechanism, and further comprising a detection mechanism disposed within the analyzer, the detection mechanism comprising:

[0007] A mounting slot, located inside the analyzer, houses rotating mounting plates arranged in a circular array. The mounting plates are fixedly connected at their closest points. Each mounting plate has a detection component for preparing the stem cell detection solution on its top. A positioning component is located on the outside of the mounting slot. The detection component includes:

[0008] A spline shaft is slidably disposed at one end of the mounting plate that is far apart from each other, and the spline shaft is capable of rotation. A connector is fixedly provided on the top of the spline shaft, and a mounting shell for mounting test tubes is fixedly provided on the top of the connector. A distance measuring sensor is fixedly provided on the side wall of the connector.

[0009] The first tooth disc is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the mounting plate.

[0010] Further, the detection assembly further comprises:

[0011] The bearing seat is fixedly sleeved on the end of the mounting plate away from each other, the inner wall of the bearing seat is provided with a bearing, the outer ring of the bearing is fixedly connected with the inner wall of the bearing seat, the inner ring of the bearing is fixedly sleeved with a spline sleeve, the inner wall of the spline sleeve is engaged with the side wall of the spline shaft, so that the spline shaft can move along the axial direction of the spline sleeve, and the spline shaft can rotate.

[0012] Further, the detection assembly further comprises:

[0013] The rotating piece is arranged on the side wall of the spline shaft and located at the bottom of the bearing seat.

[0014] The adjusting plate is sleeved on the top of the mounting plate and designed in an L shape, the horizontal end of the adjusting plate is rotatably connected with the side wall of the spline shaft through the rotating piece, and the vertical end of the adjusting plate is provided with a plurality of insertion holes arranged at intervals.

[0015] Further, the detection assembly further comprises:

[0016] The adjusting seat is fixedly arranged on the top of the mounting plate, the side wall of the adjusting seat is provided with an adjusting groove, the inner wall of the adjusting groove is designed in a special shape, the insertion rod is sleeved on the inner wall of the adjusting groove, the side wall of the insertion rod is fixedly sleeved with an insertion ring, the side wall of the insertion ring and the insertion rod respectively abuts against the special-shaped inner wall of the adjusting groove, the two ends of the insertion rod respectively extend to the two sides of the adjusting seat, the side wall of the insertion rod is matched with the inner wall of the insertion hole, and the side wall of one end of the insertion rod located in the adjusting groove and located on the side away from the insertion hole of the insertion ring is sleeved with a spring.

[0017] Further, the detection assembly further comprises:

[0018] The rubber cylinder is fixedly sleeved on the inner wall of the mounting shell, the side wall of the rubber cylinder and the mounting shell is provided with an opening, the outer side wall of the test tube is provided with a plurality of first scale lines and second scale lines arranged at intervals, the first scale lines and the second scale lines are arranged in a staggered manner, and the first scale lines and the second scale lines are towards the opening of the mounting shell.

[0019] The clamping block is fixedly arranged on the inner wall of the bottom of the mounting shell and extends into the rubber cylinder, and the bottom of the test tube is provided with a clamping groove matched with the clamping block.

[0020] Further, the detection assembly further comprises:

[0021] The inner inclined surface is arranged on the inner side wall of the test tube, so that the inner side wall of the test tube is inclined.

[0022] The first blocking ring is fixedly sleeved on the inner side wall of the test tube, and the bottom of the first blocking ring is provided with a shielding plane, and the inner wall of the first blocking ring is provided with an inclined plane.

[0023] The second blocking ring is fixedly sleeved on the inner side wall of the test tube and located at the top of the first blocking ring.

[0024] Further, the detection mechanism further comprises:

[0025] The first servo motor is fixedly arranged in the interior of the analyzer, and the output shaft of the first servo motor is fixedly provided with a first rotating shaft through a shaft coupling, and the first rotating shaft is fixedly connected with the bottom of the one end of the mounting plate close to each other, so as to drive the mounting plate to rotate;

[0026] The inner gear ring is fixedly arranged on the inner side wall of the mounting groove and matched with the first toothed disc and the second toothed disc, so that the speed of the rotation of the spline shaft is adjusted through the meshing of the first toothed disc or the second toothed disc and the inner gear ring;

[0027] The eccentric ring is fixedly arranged on the outer side of the mounting groove to determine the position of the detection assembly.

[0028] Further, the positioning assembly comprises:

[0029] The outer gear ring is rotatably arranged on the outer side of the eccentric ring, and the top of the outer gear ring is fixedly provided with a first rod body, and the sidewall of the first rod body is fixedly provided with a camera at the upper end;

[0030] The second rod body is fixedly arranged on the lower end of the sidewall of the first rod body and designed in a bent shape, and the lower end of the second rod body is fixedly provided with an identification block on the outer side.

[0031] Further, the positioning assembly further comprises:

[0032] The second servo motor is fixedly arranged in the interior of the analyzer, and the output shaft of the second servo motor is fixedly provided with a second rotating shaft through a shaft coupling, and the sidewall of the second rotating shaft is fixedly provided with a gear, and the gear is meshed with the outer gear ring;

[0033] The annular sliding block is fixedly arranged on the bottom of the outer gear ring, and the interior of the analyzer and located on the outer side of the mounting groove is provided with an annular sliding groove, and the annular sliding block is placed in the annular sliding groove to limit the rotation of the outer gear ring.

[0034] Further, the sampling mechanism comprises:

[0035] The first electric sliding rail is fixedly arranged in the interior of the analyzer, and the sliding end of the first electric sliding rail is fixedly provided with a second electric sliding rail, and the sliding end of the second electric sliding rail is fixedly provided with a third electric sliding rail, and the sliding end of the third electric sliding rail is fixedly provided with a sampling tube.

[0036] The present application provides a physical detection and analysis equipment based on stem cell characteristics. Compared with the prior art, the present application has the following advantages:

[0037] 1. This invention allows the test tube to be installed inside the analyzer via a detection mechanism, thus completing the preparation of stem cell detection solution. Compared to the traditional method of preparing stem cell detection solution, which requires opening the test tube multiple times to extract supernatant or add buffer solution, this invention simplifies the operation, improves the efficiency of stem cell detection and analysis, and prevents the stem cell suspension from being exposed to the external environment, reducing stem cell damage. It also facilitates accurate extraction of supernatant, avoiding any impact on subsequent detection and analysis.

[0038] 2. This invention utilizes the characteristic that liquid adheres tightly to the inner wall of the container under centrifugal force. By setting a first baffle ring and a shielding plane, the stem cell suspension in the test tube is restricted, reducing the probability of the stem cell suspension splashing out of the test tube during centrifugation. A second baffle ring and an inclined surface further block and guide the stem cell suspension, thereby preventing leakage of the stem cell suspension during centrifugation. This eliminates the need for the traditional method of pressing a stopper at the opening of the test tube to restrict leakage of the stem cell suspension. It is convenient to operate and facilitates sampling by the sampling mechanism, improving detection efficiency.

[0039] 3. This invention utilizes the characteristic that the distance from the center point of the eccentric ring to the inner wall is different. By monitoring the distance from the center point to the inner wall of the eccentric ring using a distance measuring sensor, the position and orientation of the test tube can be determined, so as to grasp the boundary between the supernatant and the precipitate, and so that the sampling mechanism can extract a specified amount of supernatant.

[0040] By arranging the first and second graduation lines alternately, the accuracy of the camera's acquisition is avoided from being affected by the boundary between the supernatant and the precipitate due to the graduations obscuring the boundary. It also facilitates the marking of multiple positions on the test tube and avoids the accuracy of the markings being affected by the tilt of the inner wall of the test tube.

[0041] 4. The present invention drives the test tube to revolve along the output shaft of the first servo motor by the detection component, while simultaneously causing the first or second gear disk to rotate, thereby causing the test tube to rotate. Compared with the traditional centrifugation operation, which only involves making the test tube rotate around the first servo motor, the centrifugation effect is better.

[0042] By adjusting the height of the regulating plate, the engagement between the first or second gear disc and the inner gear ring can be adjusted. By making the transmission ratio between the first or second gear disc and the inner gear ring different, the rotation speed of the test tube during revolution can be adjusted. This facilitates the adjustment of the centrifugation effect according to the specifications of the stem cell suspension, and facilitates the preparation of stem cell detection solutions of different specifications in multiple test tubes. This makes it easier to conduct subsequent detection and analysis of different stem cell detection solutions and improves the efficiency of detection and analysis. Attached Figure Description

[0043] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0044] Figure 2 Fig. 1 is a schematic diagram of the analyzer part of the present application in longitudinal section;

[0045] Figure 3 Fig. 2 is a schematic diagram of the detection mechanism of the present application;

[0046] Figure 4 Fig. 3 is a schematic diagram of the detection assembly and the inner ring gear of the present application in longitudinal section;

[0047] Figure 5 Fig. 4 is a schematic diagram of the positioning assembly and the eccentric ring of the present application in longitudinal section;

[0048] Figure 6 Fig. 5 is a schematic diagram of the eccentric ring, the annular slider and the outer ring gear of the present application in longitudinal section;

[0049] Figure 7 Fig. 6 is a schematic diagram of the identification block, the second rod body and the camera of the present application;

[0050] Figure 8 Fig. 7 is a schematic diagram of the detection assembly, the camera and the eccentric ring of the present application;

[0051] Figure 9 Fig. 8 is a schematic diagram of the inner ring gear, the mounting plate and the detection assembly of the present application;

[0052] Figure 10 Fig. 9 is a schematic diagram of the bearing seat, the mounting plate, the bearing and the spline sleeve of the present application in longitudinal section;

[0053] Figure 11 Fig. 10 is an exploded schematic diagram of the mounting plate, the spline shaft, the spline sleeve, the bearing, the bearing seat, the rotating member, the adjusting plate, the first toothed disc and the second toothed disc of the present application;

[0054] Figure 12 Fig. 11 is a schematic diagram of the mounting plate, the adjusting seat and the adjusting plate of the present application in longitudinal section;

[0055] Figure 13 Fig. 12 is a schematic diagram of the mounting shell, the connecting member and the rubber cylinder of the present application in longitudinal section;

[0056] Figure 14 Fig. 13 is a schematic diagram of the test tube of the present application in longitudinal section;

[0057] Figure 15 Fig. 14 is a schematic diagram of the sampling assembly of the present application.

[0058] The reference signs involved in the above-mentioned drawings are as follows: 1, analyzer; 2, sampling mechanism; 3, detection mechanism;

[0059] 21, third electric sliding rail; 22, sampling tube; 23, second electric sliding rail; 24, first electric sliding rail;

[0060] 31, mounting groove; 32, eccentric ring; 33, positioning assembly; 34, first servo motor; 35, mounting plate; 36, inner gear ring; 37, detection assembly;

[0061] 331, second servo motor; 332, first rod body; 333, outer gear ring; 334, second rod body; 335, camera; 336, gear; 337, annular slider; 338, identification block;

[0062] 370, first toothed disc; 371, first scale line; 372, test tube; 3721, clamping block; 3722, second blocking ring; 3723, first blocking ring; 3724, inner inclined surface; 3725, shielding plane; 3726, inclined surface; 373, mounting shell; 374, rubber cylinder; 375, second scale line; 376, connecting piece; 377, distance measuring sensor; 378, spline shaft; 379, second toothed disc; 3790, jack; 3791, spline sleeve; 3792, bearing; 3793, bearing seat; 3794, rotating piece; 3795, adjusting plate; 3796, insertion rod; 3797, adjusting seat; 3798, insertion ring; 3799, adjusting groove. DETAILED DESCRIPTION

[0063] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0064] Embodiment one: please refer to Figure 1 , Figure 2 and Figure 15 , a physical detection and analysis device based on stem cell characteristics, comprising an analyzer 1 and a sampling mechanism 2, the sampling mechanism 2 comprising:

[0065] A first electric sliding rail 24 is fixedly arranged in the interior of the analyzer 1, a sliding end of the first electric sliding rail 24 is fixedly arranged with a second electric sliding rail 23, a sliding end of the second electric sliding rail 23 is fixedly arranged with a third electric sliding rail 21, and a sliding end of the third electric sliding rail 21 is fixedly arranged with a sampling tube 22.

[0066] In specific implementation, the position of the sampling tube 22 is adjusted through the first electric sliding rail 24, the second electric sliding rail 23 and the third electric sliding rail 21, so that the sampling tube 22 can be inserted into any test tube 372, thereby facilitating the extraction of supernatant and the like.

[0067] The sampling tube 22 is connected with an external pipeline, and is extracted and discharged through the pump body. By making the external pipeline a multi-pipeline design, the sampling tube 22 can transport supernatant or stem cell detection liquid to different positions under the action of the pump body, and can add buffer liquid into the test tube 372. The connection of the external pipeline is a prior art, which will not be described here.

[0068] Please refer to Figure 2 、 Figure 3 、 Figure 4 、 Figure 9 、 Figure 10 、 Figure 11 、 Figure 12 and Figure 13 , also includes a detection mechanism 3 arranged inside the analyzer 1, the detection mechanism 3 includes:

[0069] The installation groove 31 is arranged in the inside of the analyzer 1, the inside of the installation groove 31 is rotationally provided with the installation plate 35, the installation plate 35 is arranged in an annular array, and the end of the installation plate 35 close to each other is fixedly connected, the top of the installation plate 35 is provided with a detection assembly 37 for preparing stem cell detection liquid, and the outside of the installation groove 31 is provided with a positioning assembly 33, the detection assembly 37 includes:

[0070] The spline shaft 378 is slidably arranged at the end of the installation plate 35 away from each other, and the spline shaft 378 can rotate, the top of the spline shaft 378 is fixedly provided with a connecting piece 376, the top of the connecting piece 376 is fixedly provided with an installation shell 373 for installing the test tube 372, and the side wall of the connecting piece 376 is fixedly provided with a distance measuring sensor 377.

[0071] The first tooth disc 370 is fixedly sleeved on the side wall of the spline shaft 378 and located at the bottom of the installation plate 35, and the side wall of the spline shaft 378 and located at the bottom of the first tooth disc 370 is fixedly sleeved with the second tooth disc 379.

[0072] The detection assembly 37 further includes:

[0073] The bearing seat 3793 is fixedly sleeved on the end of the installation plate 35 away from each other, the inner wall of the bearing seat 3793 is provided with the bearing 3792, the outer ring of the bearing 3792 is fixedly connected with the inner wall of the bearing seat 3793, the inner ring of the bearing 3792 is fixedly sleeved with the spline sleeve 3791, and the inner wall of the spline sleeve 3791 is engaged with the side wall of the spline shaft 378, so that the spline shaft 378 can move along the axial direction of the spline sleeve 3791, and the spline shaft 378 can rotate.

[0074] The detection assembly 37 further includes:

[0075] The rotating piece 3794 is arranged on the side wall of the spline shaft 378 and located at the bottom of the bearing seat 3793.

[0076] The adjusting plate 3795 is sleeved on the top of the mounting plate 35 and has an L-shaped design. The horizontal end of the adjusting plate 3795 is rotationally connected with the side wall of the spline shaft 378 through the rotating piece 3794. The vertical end of the adjusting plate 3795 is provided with the insertion holes 3790 arranged at intervals.

[0077] The detection assembly 37 further comprises:

[0078] The adjusting seat 3797 is fixedly arranged on the top of the mounting plate 35. The side wall of the adjusting seat 3797 is provided with the adjusting groove 3799. The inner wall of the adjusting groove 3799 has a special-shaped design. The adjusting groove 3799 is sleeved with the insertion rod 3796. The side wall of the insertion rod 3796 is fixedly sleeved with the insertion ring 3798. The side walls of the insertion ring 3798 and the insertion rod 3796 are respectively matched with the special-shaped inner wall of the adjusting groove 3799. The two ends of the insertion rod 3796 respectively extend to the two sides of the adjusting seat 3797. The side wall of the insertion rod 3796 is matched with the inner wall of the insertion hole 3790. The side wall of the one end of the insertion rod 3796 located in the adjusting groove 3799 and located on the side away from the insertion hole 3790 of the insertion ring 3798 is sleeved with the spring.

[0079] The detection assembly 37 further comprises:

[0080] The rubber cylinder 374 is fixedly sleeved on the inner wall of the mounting shell 373. The side walls of the rubber cylinder 374 and the mounting shell 373 are both provided with openings. The outer side wall of the test tube 372 is provided with the first scale line 371 and the second scale line 375 arranged at intervals. The first scale line 371 and the second scale line 375 are arranged in a staggered manner. The first scale line 371 and the second scale line 375 are towards the opening of the mounting shell 373.

[0081] The clamping block 3721 is fixedly arranged on the bottom inner wall of the mounting shell 373 and extends into the rubber cylinder 374. The bottom of the test tube 372 is provided with the clamping groove matched with the clamping block 3721.

[0082] In specific implementation, the test tube 372 containing the stem cell suspension and the serum medium is placed in the rubber cylinder 374, the test tube 372 is tightly fitted by the rubber cylinder 374, and the test tube 372 is stably installed in the mounting shell 373. After the installation of the test tube 372, the first servo motor 34 is started, the first servo motor 34 drives the mounting plate 35 to rotate through the first rotating shaft, thereby driving the spline shaft 378, the connecting piece 376 and the mounting shell 373 to rotate, and thereby driving the test tube 372 to rotate along the output shaft of the first servo motor 34. In this process, since the first tooth disc 370 or the second tooth disc 379 is engaged with the inner tooth ring 36, when the first tooth disc 370 or the second tooth disc 379 rotates along the output shaft of the first servo motor 34, the first tooth disc 370 or the second tooth disc 379 rotates to make the test tube 372 rotate. Compared with the conventional centrifugal operation in which the test tube 372 only revolves around the first servo motor 34, the centrifugal effect is better.

[0083] By inserting the plug rod 3796 into the insertion hole 3790 at different positions, the height of the adjusting plate 3795 is adjusted, and thereby the height of the spline shaft 378 is adjusted, so as to adjust the engagement between the first tooth disc 370 or the second tooth disc 379 and the inner tooth ring 36. By making the transmission ratio of the first tooth disc 370 or the second tooth disc 379 and the inner tooth ring 36 different, the rotating speed of the test tube 372 in the revolution process is adjusted, so as to facilitate the adjustment of the centrifugal effect according to the specifications of the stem cell suspension, and facilitate the configuration of stem cell detection liquids of different specifications in the plurality of test tubes 372, thereby facilitating the subsequent detection and analysis of different stem cell detection liquids, improving the efficiency of detection and analysis. In specific operation, the plug rod 3796 is pulled out of the insertion hole 3790, and then the height of the adjusting plate 3795 is adjusted. The adjusting plate 3795 drives the spline shaft 378, the first tooth disc 370 and the second tooth disc 379 to move vertically through the rotating piece 3794, so as to adjust the engagement between the first tooth disc 370 or the second tooth disc 379 and the inner tooth ring 36. After the adjustment is completed, the plug rod 3796 is inserted into the insertion hole 3790 at the corresponding position, and the plug rod 3796 is loosened. Under the action of the spring, the plug rod 3796 is stably inserted into the insertion hole 3790, so as to stably adjust the engagement between the first tooth disc 370 or the second tooth disc 379 and the inner tooth ring 36. By rotatingly connecting the adjusting plate 3795 and the spline shaft 378, the height of the spline shaft 378 is adjusted without affecting the rotation of the spline shaft 378.

[0084] The first tooth disc 370 and the second tooth disc 379 are spaced apart to ensure that the first tooth disc 370 or the second tooth disc 379 can be engaged with the inner tooth ring 36 independently, avoiding affecting the actual transmission use. The number of the first tooth disc 370 and the second tooth disc 379, and the transmission ratio of the first tooth disc 370, the second tooth disc 379 and the inner tooth ring 36 can be adjusted according to actual needs, which will not be described here.

[0085] The special-shaped inner wall of the adjusting groove 3799 is that the inner wall diameter of the adjusting groove 3799 is not the same, so as to be matched with the side wall of the insertion ring 3798 and the insertion rod 3796 respectively, so that the insertion ring 3798 is in stop cooperation with the adjusting groove 3799, so as to limit the insertion rod 3796 from being separated from the insertion groove, and the spring can elastically support the insertion rod 3796, and the stability of the insertion rod 3796 limiting the adjusting plate 3795 is improved.

[0086] The spline shaft 378 can move vertically in the spline sleeve 3791, and the mounting plate 35 is rotationally connected with the spline sleeve 3791 through the bearing seat 3793 and the bearing 3792, so that the spline shaft 378 can move vertically and can rotate.

[0087] The first scale line 371 and the second scale line 375 are staggered, that is, the scale values corresponding to the first scale line 371 and the second scale line 375 have a difference, for example, the first scale line 371 displays 7mm, and the second scale line 375 limits 5mm, that is, there is a difference of 2mm between the first scale line 371 and the second scale line 375, so that the first scale line 371 and the second scale line 375 are staggered, so as to avoid affecting the accuracy of the camera 335 collecting due to the scale shielding the boundary of the supernatant and the precipitate, and the first scale line 371 and the second scale line 375 are convenient for scale identification of multiple positions of the test tube 372, and the accuracy of scale identification is affected by the inner wall of the test tube 372 being inclined.

[0088] The first rotating shaft is rotationally connected with the analyzer 1, so as to ensure the stability of the rotation of the first rotating shaft.

[0089] The analyzer 1 is internally provided with temperature control equipment such as a heater and a temperature sensor, so as to control the temperature of the stem cell suspension, which is prior art and is not shown in the figure and will not be described here.

[0090] Please refer to Figure 14 , the detection assembly 37 further comprises:

[0091] The inner inclined surface 3724 is arranged on the inner side wall of the test tube 372, so that the inner side wall of the test tube 372 is inclined;

[0092] The first blocking ring 3723 is fixedly sleeved on the inner side wall of the test tube 372, and the bottom of the first blocking ring 3723 is provided with a shielding plane 3725, and the inner wall of the first blocking ring 3723 is provided with an inclined surface 3726;

[0093] The second blocking ring 3722 is fixedly sleeved on the inner side wall of the test tube 372 and located at the top of the first blocking ring 3723.

[0094] In the specific implementation, when centrifuging the test tube 372, the stem cell suspension in the test tube 372 adheres to the inner side wall of the test tube 372 due to the centrifugal force, so as to limit the stem cell suspension in the test tube 372 by blocking the movement track of the stem cell suspension through the first blocking ring 3723 and the blocking plane 3725, thereby reducing the probability of the stem cell suspension spilling out of the test tube 372 during the centrifugation, and part of the stem cell suspension spilling out along the inner wall of the first blocking ring 3723 enters between the first blocking ring 3723 and the second blocking ring 3722 and is blocked again by the second blocking ring 3722. In addition, the stem cell suspension is guided to the bottom of the first blocking ring 3723 through the inclined plane 3726, so as to avoid the stem cell suspension leaking out during the centrifugation without pressing the plug column at the opening of the test tube 372 to limit the stem cell suspension in the test tube 372 from leaking out, which facilitates the operation and sampling by the sampling mechanism 2 and improves the detection efficiency.

[0095] During the centrifugation, the start and stop of the centrifugal motion need to be performed slowly to avoid causing the stem cell suspension in the test tube 372 to splash out of the test tube 372.

[0096] By arranging the inner inclined surface 3724, the inner side wall of the test tube 372 is designed to be inclined, which improves the centrifugation effect compared with the traditional method of inclining the test tube 372 during the centrifugation and then restoring the test tube 372 to be vertical after the centrifugation, and the test tube 372 is always vertical, which facilitates the actual sampling use, and the inclination angle of the inner inclined surface 3724 is arranged to be less than the center line of the test tube 372, so as not to affect the actual sampling use of the sampling tube 22.

[0097] During the installation of the test tube 372, the test tube 372 is extruded by the rubber cylinder 374 to improve the stability of the installation of the test tube 372, and the clamping block 3721 is embedded in the clamping groove, so as to limit the position of the test tube 372, and the first scale line 371 and the second scale line 375 are directed to the opening of the installation shell 373 and the rubber cylinder 374, so as to facilitate the subsequent observation of the boundary between the supernatant and the precipitate of the stem cell suspension after the centrifugation by the camera 335, thereby facilitating the sampling mechanism 2 to take out the supernatant, and due to the arrangement of the inner inclined surface 3724, the bottom of the test tube 372 has a space for the clamping groove.

[0098] Please refer to Figure 2 , Figure 3 , Figure 9 and Figure 10 , the detection mechanism 3 further comprises:

[0099] The first servo motor 34 is fixedly arranged in the analyzer 1. An output shaft of the first servo motor 34 is fixedly arranged with a first rotating shaft through a shaft coupling. The first rotating shaft is fixedly connected to the bottom of the one end of the mounting plate 35 close to each other, so as to drive the mounting plate 35 to rotate. The first servo motor 34 is convenient for driving the mounting plate 35 to rotate, so as to conveniently drive the test tube 372 to rotate.

[0100] The inner gear ring 36 is fixedly arranged on the inner side wall of the mounting groove 31, and is matched with the first tooth disc 370 and the second tooth disc 379, so as to adjust the speed of the rotation of the spline shaft 378 through the meshing of the first tooth disc 370 or the second tooth disc 379 and the inner gear ring 36.

[0101] The eccentric ring 32 is fixedly arranged on the outer side of the mounting groove 31, so as to determine the position of the detection assembly 37. Through the feature that the distance from the center point of the eccentric ring 32 to the inner wall is different, when the centrifugation is completed, the distance from the inner wall of the eccentric ring 32 to the detection assembly 37 is monitored by the distance measuring sensor 377, so as to determine the position of the test tube 372. The first scale line 371 and the second scale line 375 are towards the inner wall of the eccentric ring 32, so as to cooperate with the identification block 338 of the positioning assembly 33, so as to conveniently grasp the position of the test tube 372. Through the camera 335, the boundary of the supernatant and the precipitate is conveniently grasped, so as to facilitate the sampling mechanism 2 to extract a specified amount of supernatant.

[0102] Embodiment two: please refer to Figure 5 、 Figure 6 、 Figure 7 and Figure 8 The difference between the technical scheme of the embodiment and the embodiment one is that the positioning assembly 33 comprises:

[0103] The outer gear ring 333 is rotatably arranged on the outer side of the eccentric ring 32. The top of the outer gear ring 333 is fixedly arranged with the first rod body 332. The side wall upper end of the first rod body 332 is fixedly arranged with the camera 335.

[0104] The second rod body 334 is fixedly arranged on the side wall lower end of the first rod body 332, and is designed in a bent shape. The second rod body 334 is fixedly arranged with the identification block 338 on the outer side of the lower end.

[0105] The positioning assembly 33 further comprises:

[0106] The second servo motor 331 is fixedly arranged in the analyzer 1. The output shaft of the second servo motor 331 is fixedly arranged with a second rotating shaft through a shaft coupling. The side wall of the second rotating shaft is fixedly arranged with the gear 336. The gear 336 is meshed with the outer gear ring 333.

[0107] The annular sliding block 337 is fixedly arranged on the bottom of the outer gear ring 333. The inside of the analyzer 1 and the outer side of the mounting groove 31 are provided with an annular sliding groove. The annular sliding block 337 is placed in the annular sliding groove, so as to limit the rotation of the outer gear ring 333.

[0108] In the specific implementation, after the position of the test tube 372 is grasped by the eccentric ring 32 and the distance measuring sensor 377, the second servo motor 331 drives the gear 336 to rotate, the gear 336 drives the outer gear ring 333 to rotate under the limiting of the annular slider 337 and the annular sliding groove, so as to adjust the position of the camera 335, so that the camera 335 collects the boundary between the supernatant and the precipitate, and the sampling mechanism 2 is convenient for sampling.

[0109] When the camera 335 approaches the target test tube 372, the distance measuring sensor 377 measures the distance of the identification block 338, so as to further confirm the position of the test tube 372 and improve the accuracy.

[0110] The first servo motor 34 and the second servo motor 331 and the like of the present application are connected with the controller and the external power supply through wires, so as to facilitate actual control and use, which is the prior art and will not be described here.

[0111] In the implementation of the present application, the stem cell suspension is placed in the test tube 372 and is thawed, after the thawing is completed, the serum medium is added, then the door body of the analyzer 1 is opened, the thawed stem cell suspension is placed in the mounting shell 373, and the first scale line 371 and the second scale line 375 are limited to the opening of the mounting shell 373 through the clamping block 3721 and the clamping groove, then the door body is closed, and the first servo motor 34 is started to drive the detection assembly 37 arranged in the annular array to do annular motion along the output shaft of the first servo motor 34, so that the test tube 372 does annular motion along the output shaft of the first servo motor 34, and at the same time, the test tube 372 is rotated through the cooperation of the inner gear ring 36, the first tooth disc 370 and the second tooth disc 379, compared with the transmission which can only make the test tube 372 revolve along the output shaft of the first servo motor, the centrifugal effect of the stem cell suspension in the test tube 372 is improved, after the centrifugation is completed, the position of the test tube 372 is determined through the cooperation of the distance measuring sensor 377 and the eccentric ring 32, and the orientations of the first test tube 372 and the second test tube 372 are determined, so as to facilitate the determination of the boundary position of the supernatant and the precipitate through the cooperation with the positioning assembly 33, and then the sampling mechanism 2 is convenient for accurately taking out the supernatant, after the supernatant is taken out, the buffer solution is added into the test tube 372 through the sampling mechanism 2, and the supernatant and the precipitate are obtained through the above-mentioned centrifugal method, so as to wash the stem cells, after multiple washing, the stem cell detector is obtained, and the stem cell detection liquid is delivered to the analysis position through the sampling mechanism 2, so as to detect and analyze the stem cells.

[0112] Meanwhile, the contents not described in detail in the present specification all belong to the prior art known by those skilled in the art.

[0113] It is to be understood that the terminology "first" and "second", and the like, used in the context of the present specification are merely used to differentiate one entity or action from another without necessarily implying any actual relationship or order between or among the entities or actions.

[0114] While the embodiments of the application have been illustrated and described, it will be understood by those skilled in the art that various changes, modifications, substitutions, and alterations can be made therein without departing from the spirit and scope of the application as defined by the appended claims and their equivalents.

Claims

1. A physical detection and analysis device based on stem cell characteristics, comprising an analyzer and a sampling mechanism, characterized in that, It also includes a detection mechanism located inside the analyzer, which includes: A mounting slot, located inside the analyzer, houses rotating mounting plates arranged in a circular array. The mounting plates are fixedly connected at their closest points. Each mounting plate has a detection component for preparing the stem cell detection solution on its top. A positioning component is located on the outside of the mounting slot. The detection component includes: A spline shaft is slidably disposed at one end of the mounting plate that is far apart from each other, and the spline shaft is capable of rotation. A connector is fixedly provided on the top of the spline shaft, and a mounting shell for mounting test tubes is fixedly provided on the top of the connector. A distance measuring sensor is fixedly provided on the side wall of the connector. The first gear is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the mounting plate. The second gear is fixedly sleeved on the side wall of the spline shaft and located at the bottom of the first gear. The detection component also includes: An inner bevel is formed on the inner wall of a test tube to make the inner wall of the test tube inclined. The first retaining ring is fixedly sleeved on the inner wall of the test tube. The bottom of the first retaining ring is provided with a shielding plane, and the inner wall of the first retaining ring is provided with an inclined surface. The second retaining ring is fixedly sleeved on the inner side wall of the test tube and is located at the top of the first retaining ring; The testing institution also includes: The first servo motor is fixedly installed inside the analyzer. The output shaft of the first servo motor is fixedly connected to the first rotating shaft via a coupling. The bottom of the first rotating shaft is fixedly connected to the mounting plate at one end, so as to drive the mounting plate to rotate. An internal gear ring is fixedly mounted on the inner side wall of the mounting groove and is adapted to the first gear plate and the second gear plate so as to adjust the speed of the spline shaft rotation by meshing with the internal gear ring through the first gear plate or the second gear plate. An eccentric ring is fixedly installed on the outside of the mounting groove to determine the position of the detection component; The sampling mechanism includes: The first electric slide rail is fixedly installed inside the analyzer. The sliding end of the first electric slide rail is fixedly provided with the second electric slide rail. The sliding end of the second electric slide rail is fixedly provided with the third electric slide rail. The sliding end of the third electric slide rail is fixedly provided with the sampling tube. The positioning component includes: An external gear ring is rotatably located on the outside of the eccentric ring. A first rod is fixedly mounted on the top of the external gear ring, and a camera is fixedly mounted on the upper side wall of the first rod. The second rod is fixed to the lower end of the side wall of the first rod and is designed in a bent shape. A marker block is fixed to the outer side of the lower end of the second rod.

2. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that, The detection component also includes: The bearing housing is fixedly sleeved at one end of the mounting plate away from each other. The bearing is installed on the inner wall of the bearing housing. The outer ring of the bearing is fixedly connected to the inner wall of the bearing housing. A spline sleeve is fixedly sleeved on the inner ring of the bearing. The inner wall of the spline sleeve meshes with the side wall of the spline shaft, so that the spline shaft can move along the axial direction of the spline sleeve and can rotate on its own axis.

3. The physical detection and analysis device based on stem cell characteristics according to claim 2, characterized in that, The detection component also includes: The rotating component is located on the side wall of the splined shaft and at the bottom of the bearing housing; An adjusting plate is fitted onto the top of the mounting plate and is designed in an L-shape. The horizontal end of the adjusting plate is rotatably connected to the side wall of the spline shaft through a rotating component, and the vertical end of the adjusting plate has spaced insertion holes.

4. The physical detection and analysis device based on stem cell characteristics according to claim 3, characterized in that, The detection component also includes: An adjusting seat is fixedly mounted on the top of the mounting plate. The side wall of the adjusting seat has an adjusting groove with an irregularly shaped inner wall. A rod is fitted into the inner wall of the adjusting groove, and a ring is fixedly fitted into the side wall of the rod. The side walls of the ring and the rod are respectively fitted to the irregularly shaped inner wall of the adjusting groove. The two ends of the rod extend to both sides of the adjusting seat. The side wall of the rod is adapted to the inner wall of the insertion hole. A spring is fitted into the side wall of the rod located in the adjusting groove, on the side of the ring away from the insertion hole.

5. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that, The detection component also includes: A rubber tube is fixedly sleeved on the inner wall of the mounting shell. Both the rubber tube and the side wall of the mounting shell have openings. The outer wall of the test tube is provided with a first scale line and a second scale line arranged at intervals. The first scale line and the second scale line are staggered and face the opening of the mounting shell. The locking block is fixedly installed on the bottom inner wall of the mounting shell and extends into the rubber cylinder. The bottom of the test tube is provided with a locking groove that matches the locking block.

6. The physical detection and analysis device based on stem cell characteristics according to claim 1, characterized in that, The positioning component also includes: The second servo motor is fixed inside the analyzer. The output shaft of the second servo motor is fixed to the second rotating shaft through a coupling. A gear is fixed to the side wall of the second rotating shaft, and the gear meshes with the external gear ring. An annular slider is fixedly mounted at the bottom of the outer gear ring. An annular groove is provided inside the analyzer and outside the mounting slot. The annular slider is placed in the annular groove to limit the rotation of the outer gear ring.

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