Formula of casein tryptone agar culture medium

Abstract

The invention discloses a casein tryptone agar culture medium formula, and particularly relates to the technical field of culture media, and the casein tryptone agar culture medium formula comprises the following components by weight: 15-17 g of casein pancreatin digest, 3-5 g of soy peptone, 5-6 g of sodium chloride, 14-15 g of agar, 5-6 g of glucose, 0.5-0.6 g of glutamic acid, 0.3-0.5 g of vitamin B family, 0.1-0.2 g of ascorbic acid, 10-15 g of a phosphate buffer solution, and 2-3 g of an antioxidant. The high survival rate of microorganisms can be guaranteed, nutrients for survival and growth can be well provided for the microorganisms, and the actual using effect of the culture medium is good.

Description

Technical Field

[0001] The present invention relates to the technical field of culture medium, and more particularly to a trypticase soy agar culture medium formula. Background Art

[0002] Trypticase soy peptone agar medium, also known as TSA medium, is a widely used microbial culture medium. The main components of trypticase soy peptone agar medium include trypticase peptone, soy peptone (or soy papain hydrolysate), sodium chloride and agar. These components provide the nutrients such as nitrogen source, carbon source, vitamins and growth factors required for microbial growth.

[0003] When culturing microorganisms using the tryptic soy agar medium in the prior art, problems such as nutrient deficiency and low microbial survival rate easily occur. Therefore, a tryptic soy agar medium formula is urgently needed to solve this problem. Summary of the Invention

[0004] In order to overcome the above-mentioned defects of the prior art, an embodiment of the present invention provides a tryptic soy peptone agar culture medium formula, which can ensure a high survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms, so that the actual use effect of the present invention is better, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical solution: a tryptic soy agar culture medium formula, comprising the following components by weight: 15-17g pancreatic digest of casein; Soy peptone 3-5g; Sodium chloride 5-6g; Agar 14-15g; Glucose 5-6g; Glutamic acid 0.5-0.6g; Vitamin B group 0.3-0.5g; Ascorbic acid 0.1-0.2g; Phosphate buffer 10-15g; Antioxidants 2-3g.

[0006] In a preferred embodiment, the glucose is used to provide more energy to promote the growth and survival of microorganisms.

[0007] In a preferred embodiment, the glutamic acid is used to promote protein synthesis by microorganisms and enhance their growth ability.

[0008] In a preferred embodiment, the B vitamins are used to maintain cell growth.

[0009] In a preferred embodiment, the ascorbic acid is used to protect microorganisms from oxidative stress, improve bacterial activity and product synthesis efficiency, and at the same time, acts as an electron donor, participates in the electron transport chain of microorganisms, and promotes energy metabolism.

[0010] In a preferred embodiment, the phosphate buffer is used to maintain a stable pH value of the culture medium.

[0011] In a preferred embodiment, the antioxidant is used to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0012] The technical effects and advantages of the present invention are as follows: The invention comprises 15-17g of casein pancreatic digest, 3-5g of soy peptone, 5-6g of sodium chloride, 14-15g of agar, 5-6g of glucose, 0.5-0.6g of glutamic acid, 0.3-0.5g of B vitamins, 0.1-0.2g of ascorbic acid, 10-15g of phosphate buffer, and 2-3g of antioxidant. The glucose can provide more energy and promote the growth and survival of microorganisms; the glutamic acid can promote protein synthesis of microorganisms and enhance growth ability; the B vitamins can maintain cell growth; the ascorbic acid can protect microorganisms from oxidative stress and improve bacterial activity and product synthesis efficiency; and at the same time, as an electron donor, participates in the electron transport chain of microorganisms and promotes energy metabolism; the phosphate buffer can maintain the pH value of the culture medium stable; and the antioxidant can protect microorganisms from damage by oxygen free radicals and other oxidative stress factors. The invention can ensure a high survival rate of microorganisms and can better provide nutrients for survival and growth of microorganisms, so that the actual use effect of the invention is better. DETAILED DESCRIPTION

[0013] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.

[0014] Example 1 The present invention provides a tryptic soy agar culture medium formula, comprising the following components by weight: 15g pancreatic digest of casein; Soy peptone 3g; Sodium chloride 5g; Agar 14g; Glucose 5g; Glutamic acid 0.5g; Vitamin B group 0.3g; Ascorbic acid 0.1g; Phosphate buffer 10g; Antioxidant 2g.

[0015] The glucose is used to provide more energy and promote the growth and survival of microorganisms.

[0016] The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

[0017] The B vitamins are used to maintain cell growth.

[0018] The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

[0019] The phosphate buffer is used to maintain a stable pH value of the culture medium.

[0020] The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0021] Compared with the prior art, the trypticase soy agar culture medium prepared in this embodiment can ensure a higher survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms.

[0022] Example 2 The present invention provides a tryptic soy agar culture medium formula, comprising the following components by weight: 15.5g pancreatic digest of casein; Soy peptone 3.5g; Sodium chloride 5g; Agar 14g; Glucose 5g; Glutamic acid 0.5g; Vitamin B group 0.35g; Ascorbic acid 0.1g; Phosphate buffer 11g; Antioxidant 2.25g.

[0023] The glucose is used to provide more energy and promote the growth and survival of microorganisms.

[0024] The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

[0025] The B vitamins are used to maintain cell growth.

[0026] The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

[0027] The phosphate buffer is used to maintain a stable pH value of the culture medium.

[0028] The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0029] Compared with the prior art, the trypticase soy agar culture medium prepared in this embodiment can ensure a higher survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms.

[0030] Example 3 The present invention provides a tryptic soy agar culture medium formula, comprising the following components by weight: 16g pancreatic digest of casein; Soy peptone 4g; Sodium chloride 5.5g; Agar 14.5g; Glucose 5.5g; Glutamic acid 0.55g; Vitamin B group 0.4g; Ascorbic acid 0.15g; Phosphate buffer 12g; Antioxidant 2.5g.

[0031] The glucose is used to provide more energy and promote the growth and survival of microorganisms.

[0032] The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

[0033] The B vitamins are used to maintain cell growth.

[0034] The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

[0035] The phosphate buffer is used to maintain a stable pH value of the culture medium.

[0036] The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0037] Compared with the prior art, the trypticase soy agar culture medium prepared in this embodiment can ensure a higher survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms.

[0038] Example 4 The present invention provides a tryptic soy agar culture medium formula, comprising the following components by weight: Pancreatic digest of casein 16.5g; Soy peptone 4.5g; Sodium chloride 6g; Agar 15g; Glucose 6g; Glutamic acid 0.6g; Vitamin B group 0.45g; Ascorbic acid 0.2g; Phosphate buffer 14g; Antioxidant 2.75g.

[0039] The glucose is used to provide more energy and promote the growth and survival of microorganisms.

[0040] The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

[0041] The B vitamins are used to maintain cell growth.

[0042] The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

[0043] The phosphate buffer is used to maintain a stable pH value of the culture medium.

[0044] The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0045] Compared with the prior art, the trypticase soy agar culture medium prepared in this embodiment can ensure a higher survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms.

[0046] Example 5 The present invention provides a tryptic soy agar culture medium formula, comprising the following components by weight: 17g pancreatic digest of casein; Soy peptone 5g; Sodium chloride 6g; Agar 15g; Glucose 6g; Glutamic acid 0.6g; Vitamin B group 0.5g; Ascorbic acid 0.2g; Phosphate buffer 15g; Antioxidant 3g.

[0047] The glucose is used to provide more energy and promote the growth and survival of microorganisms.

[0048] The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

[0049] The B vitamins are used to maintain cell growth.

[0050] The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

[0051] The phosphate buffer is used to maintain a stable pH value of the culture medium.

[0052] The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.

[0053] Compared with the prior art, the trypticase soy agar culture medium prepared in this embodiment can ensure a higher survival rate of microorganisms and can better provide nutrients for the survival and growth of microorganisms.

[0054] The invention comprises 15-17g of casein pancreatic digest, 3-5g of soy peptone, 5-6g of sodium chloride, 14-15g of agar, 5-6g of glucose, 0.5-0.6g of glutamic acid, 0.3-0.5g of B vitamins, 0.1-0.2g of ascorbic acid, 10-15g of phosphate buffer, and 2-3g of antioxidant. The glucose can provide more energy and promote the growth and survival of microorganisms; the glutamic acid can promote protein synthesis of microorganisms and enhance growth ability; the B vitamins can maintain cell growth; the ascorbic acid can protect microorganisms from oxidative stress and improve bacterial activity and product synthesis efficiency; and at the same time, as an electron donor, participates in the electron transport chain of microorganisms and promotes energy metabolism; the phosphate buffer can maintain the pH value of the culture medium stable; and the antioxidant can protect microorganisms from damage by oxygen free radicals and other oxidative stress factors. The invention can ensure a high survival rate of microorganisms and can better provide nutrients for survival and growth of microorganisms, so that the actual use effect of the invention is better.

[0055] Finally: The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A tryptic soy agar medium formula, characterized in that: The following components are included by weight: 15-17g pancreatic digest of casein; Soy peptone 3-5g; Sodium chloride 5-6g; Agar 14-15g; Glucose 5-6g; Glutamic acid 0.5-0.6g; Vitamin B group 0.3-0.5g; Ascorbic acid 0.1-0.2g; Phosphate buffer 10-15g; Antioxidants 2-3g.

2. A tryptic soy agar medium formulation according to claim 1, characterized in that: The glucose is used to provide more energy and promote the growth and survival of microorganisms.

3. A tryptic soy agar medium formulation according to claim 1, characterized in that: The glutamic acid is used to promote protein synthesis of microorganisms and enhance growth ability.

4. A tryptic soy agar medium formulation according to claim 1, characterized in that: The B vitamins are used to maintain cell growth.

5. A tryptic soy agar medium formulation according to claim 1, characterized in that: The ascorbic acid is used to protect microorganisms from oxidative stress, improve the activity of the microorganism and the efficiency of product synthesis, and at the same time, acts as an electron donor, participates in the electron transport chain of the microorganism, and promotes energy metabolism.

6. A tryptic soy agar medium formulation according to claim 1, characterized in that: The phosphate buffer is used to maintain a stable pH value of the culture medium.

7. The tryptic soy agar medium according to claim 1, wherein: The antioxidants serve to protect microorganisms from damage by oxygen free radicals and other oxidative stress factors.