Zebra fish anesthesia imaging auxiliary system and method

Abstract

The invention discloses a zebra fish anesthesia imaging auxiliary system and method, and the system comprises a water chamber which is provided with a cover body; a water inlet is formed in the cover body, and an auxiliary opening and a water outlet are formed in the water chamber; a fixed bracket is arranged in the water chamber; the water chamber is made of a non-metal material; a water injection pool; a circulating water pump; a front temperature sensor; a rear temperature sensor; a temperature control module; the control module is electrically connected with the front temperature sensor, the rear temperature sensor and the temperature control module and used for controlling the temperature control module to adjust the water temperature state in the water chamber so as to maintain the anesthesia state of the zebra fish to be experimented; wherein under the condition that the zebra fish anesthesia imaging auxiliary system is used for nuclear magnetic resonance imaging, the water injection pool, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all arranged far away from nuclear magnetic resonance equipment. The zebra fish imaging assistance suitable for nuclear magnetic resonance imaging can be provided, the safety of the zebra fish anesthesia process is improved, and the imaging stability is guaranteed.

Description

Technical Field

[0001] The present application relates to the field of medical imaging technology, and in particular to a zebrafish anesthesia imaging assistance system and method. Background Art

[0002] Imaging and studying living organisms is of great significance in biomedical research. As an important model organism, zebrafish are widely used in research in biology, medicine, and other fields. When studying zebrafish, live imaging is necessary to observe changes in their bodies. At the same time, anesthesia is required to reduce stress responses during imaging.

[0003] Currently, there are relatively few technologies for zebrafish imaging and anesthesia, and there are relatively few existing zebrafish imaging devices. These primarily work by imaging zebrafish using optical techniques. However, optical imaging is more suitable for dynamic fluorescence monitoring after molecular labeling and has poor sensitivity for imaging tissue or surrounding lesions. Magnetic resonance imaging (MRI) offers significant advantages in imaging deep tissues and identifying soft tissue lesions. However, there is currently a lack of zebrafish imaging assistance systems suitable for MRI. Summary of the Invention

[0004] This application aims to propose a zebrafish anesthesia imaging assistance system and method, which can provide zebrafish imaging assistance suitable for magnetic resonance imaging, improve the safety of zebrafish during anesthesia, and ensure the stability of the imaging process.

[0005] The zebrafish anesthesia imaging assistance system according to the first aspect of the present application includes:

[0006] A water tank having a cover for sealing the water tank; a water inlet is provided on the cover, and an auxiliary port and a water outlet are provided on the water tank, wherein the water outlet is located at an end of the water tank away from the cover; a fixing bracket is provided in the water tank, wherein the fixing bracket is used to fix the zebrafish to be tested, and the auxiliary port is used to adjust the body position of the zebrafish to be tested; the water tank is made of non-metallic material;

[0007] a water injection tank having an output port in communication with the water inlet and an input port in communication with the water outlet;

[0008] a circulating water pump, for circulating water between the water tank and the water injection tank;

[0009] A front temperature sensor, used to collect the inlet water temperature of the water inlet;

[0010] A rear temperature sensor is used to collect the outlet water temperature of the water outlet;

[0011] a temperature control module, for regulating the temperature of water flowing through the water tank and the water injection tank;

[0012] a control module, electrically connected to the front temperature sensor, the rear temperature sensor, and the temperature control module, and configured to control the temperature control module to adjust the water temperature inside the water tank according to the inlet water temperature and the outlet water temperature, so as to maintain the anesthesia state of the zebrafish to be tested;

[0013] Among them, when the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all arranged away from the magnetic resonance imaging equipment.

[0014] According to some embodiments of the present application, the zebrafish anesthesia imaging assistance system further includes:

[0015] The water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all arranged in the box;

[0016] Wherein, when the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the box is arranged away from the magnetic resonance imaging device.

[0017] According to some embodiments of the present application, when the zebrafish anesthesia imaging assistance system is used for magnetic resonance imaging, the water tank is set within the detection range of the magnetic resonance imaging device.

[0018] According to some embodiments of the present application, there are multiple water tanks, and the multiple water tanks are connected in series in sequence through the water inlet and the water outlet.

[0019] According to some embodiments of the present application, the water tank further includes:

[0020] The partition is used to separate the water tank into an upper chamber and a lower chamber. The fixing bracket is arranged in the upper chamber. The partition is provided with a water hole.

[0021] According to some embodiments of the present application, the fixed support includes a first adjustable support frame and a second adjustable support frame, and the zebrafish to be tested is placed on the first adjustable support frame and the second adjustable support frame, and the first adjustable support frame and the second adjustable support frame are respectively used to adjust the height of both sides of the zebrafish to be tested.

[0022] According to some embodiments of the present application, the temperature control module includes a heating unit and a cooling unit both electrically connected to the control module.

[0023] The zebrafish anesthesia imaging assistance method according to the second embodiment of the present application is applied to the zebrafish anesthesia imaging assistance system as described in the first embodiment, including:

[0024] Obtaining the inlet water temperature and the outlet water temperature of the water tank, wherein the water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all pre-installed away from the nuclear magnetic resonance equipment;

[0025] Obtaining a current predicted water temperature in the water tank according to the inlet water temperature and the outlet water temperature, and adjusting the temperature of the water flowing through the water tank so that the water temperature in the water tank is within a preset temperature range;

[0026] When the current predicted water flow temperature in the cabin is within the preset temperature range, an anesthesia imaging operation is performed.

[0027] According to some embodiments of the present application, obtaining the current predicted water temperature in the water tank according to the inlet water temperature and the outlet water temperature, and adjusting the temperature of the water flowing through the water tank so that the water temperature in the water tank is within a preset temperature range, includes:

[0028] When the predicted water temperature in the water tank is higher than a preset high temperature threshold or lower than a preset low temperature threshold, the circulating water pump is turned off, and the temperature control module is controlled to operate so as to adjust the water temperature in the water tank so that the water temperature in the water tank tends to the preset temperature range; the preset low temperature threshold is lower than the lower threshold value of the preset temperature range, and the preset high temperature threshold is higher than the upper threshold value of the preset temperature range;

[0029] When the predicted water temperature in the water tank exceeds the preset temperature range and is within the constraint range of the preset low temperature threshold and the preset high temperature threshold, the temperature control module is controlled to operate to adjust the water temperature in the water tank so that the water temperature in the water tank tends to the preset temperature range.

[0030] According to some embodiments of the present application, performing the anesthesia imaging operation when the current predicted water flow temperature in the cabin is within the preset temperature range includes:

[0031] When the current predicted water flow temperature in the water tank is within the preset temperature range, placing the zebrafish to be tested into the water tank;

[0032] Adjusting the fixing bracket to fix the zebrafish to be tested;

[0033] Injecting air into the water tank through the auxiliary port to adjust the zebrafish to a body position required for imaging;

[0034] The water tank is placed within the detection range of the nuclear magnetic resonance device to perform an anesthesia imaging operation.

[0035] In the embodiment of the present application, by reasonably arranging the positions of each module and using a water tank made of non-metallic materials to place the zebrafish to be tested, the zebrafish anesthesia imaging assistance system of the present application can be applied to magnetic resonance imaging of zebrafish, solving the problem that the existing zebrafish imaging device is difficult to obtain good imaging effects and cannot clearly display the changes in the zebrafish body under the magnetic resonance environment; through the front temperature sensor, the rear temperature sensor, the temperature control module and the control module, and in conjunction with the zebrafish anesthesia imaging assistance method, high-precision water temperature control in the water tank can be achieved, thereby achieving anesthesia assistance for the zebrafish, ensuring the safety of the zebrafish during the anesthesia process, and ensuring the stability of the imaging process; therefore, the present application provides an efficient and safe technical platform for in vivo biomedical research.

[0036] Other features and advantages of the present application will be set forth in the following description, and in part will be apparent from the description, or may be learned by practicing the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0038] Figure 1 is a structural block diagram of an embodiment of the zebrafish anesthesia imaging assistance system of the present application;

[0039] Figure 2 This is a partial structural diagram of another embodiment of the zebrafish anesthesia imaging assistance system of the present application;

[0040] Figure 3 1 is a schematic structural diagram of a water tank in an embodiment of the zebrafish anesthesia imaging assistance system of the present application;

[0041] Figure 4 This is a schematic structural diagram from another perspective of the water tank of an embodiment of the zebrafish anesthesia imaging assistance system of the present application.

[0042] Reference numerals:

[0043] Water tank 100, cover 110, water inlet 120, water outlet 130, auxiliary port 140, fixing bracket 150, partition 160, water hole 161,

[0044] Water injection tank 200, regulating water tank 210,

[0045] Circulating water pump 300,

[0046] Front temperature sensor 400,

[0047] Rear temperature sensor 500,

[0048] That is, hot water pipe 610, anti-dry temperature sensor 611, cooling water pipe 620, semiconductor cooling sheet 621, heat dissipation fin 622, cooling fan 623,

[0049] High temperature sensor 710, low temperature sensor 720,

[0050] Control module 800, display unit 810,

[0051] Box 900. DETAILED DESCRIPTION

[0052] The following describes in detail embodiments of the present application. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application and are not to be construed as limiting the present application.

[0053] In the description of this application, if there is a description of first, second, etc., it is only for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0054] In the description of this application, it should be understood that descriptions involving orientation, such as the orientation or positional relationship indicated by up, down, etc., are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0055] In the description of this application, it should be noted that, unless otherwise clearly defined, terms such as setting, installing, and connecting should be understood in a broad sense, and technical personnel in the relevant technical field can reasonably determine the specific meaning of the above terms in this application based on the specific content of the technical solution.

[0056] The technical solution of the present application will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the embodiments described below are only part of the embodiments of the present application, not all of the embodiments.

[0057] Figure 1 is a structural block diagram of an embodiment of the zebrafish anesthesia imaging assistance system of the present application; Figure 2 1 is a schematic structural diagram of a water tank in an embodiment of the zebrafish anesthesia imaging assistance system of the present application; Figure 3 This is another perspective structural diagram of the water tank of the embodiment of the zebrafish anesthesia imaging auxiliary system of the present application. Figures 1 to 3, further elaborating on the embodiments of this application.

[0058] like Figure 1 As shown, the embodiment of the present application proposes a zebrafish anesthesia imaging assistance system, which includes:

[0059] The water tank 100 has a cover 110 for sealing the water tank 100; the cover 110 is provided with a water inlet 120, and the water tank 100 is provided with an auxiliary port 140 and a water outlet 130, with the water outlet 130 being located at an end of the water tank 100 away from the cover 110; a fixing bracket 150 is provided in the water tank 100, the fixing bracket 150 is used to fix the zebrafish to be tested, and the auxiliary port 140 is used to adjust the body position of the zebrafish to be tested; the water tank 100 is made of non-metallic material;

[0060] The water injection tank 200 has an output port communicating with the water inlet 120 and an input port communicating with the water outlet 130;

[0061] A circulating water pump 300 is used to circulate water between the water tank 100 and the water injection tank 200;

[0062] The front temperature sensor 400 is used to collect the inlet water temperature of the water inlet 120;

[0063] The rear temperature sensor 500 is used to collect the outlet water temperature of the water outlet 130;

[0064] A temperature control module, used to adjust the temperature of the water flowing through the water tank 100 and the water injection tank 200;

[0065] The control module 800 is electrically connected to the front temperature sensor 400, the rear temperature sensor 500, and the temperature control module, and is used to control the temperature control module to adjust the water temperature inside the water tank 100 according to the inlet water temperature and the outlet water temperature to maintain the anesthesia state of the zebrafish to be tested;

[0066] Among them, when the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the water injection tank 200, the circulating water pump 300, the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800 are all set away from the magnetic resonance imaging equipment.

[0067] In the embodiment of the present application, by reasonably arranging the positions of each module and using a water tank 100 made of non-metallic material to place the zebrafish to be tested, the zebrafish anesthesia imaging assistance system of the present application can be applied to magnetic resonance imaging of zebrafish, solving the problem that the existing zebrafish imaging device is difficult to obtain good imaging effects and cannot clearly display the changes in the zebrafish body under the magnetic resonance environment; through the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800, and in conjunction with the zebrafish anesthesia imaging assistance method, high-precision water temperature control in the water tank 100 can be achieved, thereby achieving anesthesia assistance for the zebrafish, ensuring the safety of the zebrafish during the anesthesia process, and ensuring the stability of the imaging process; therefore, the present application provides an efficient and safe technical platform for in vivo biomedical research.

[0068] The water tank 100, the circulating water pump 300 and the water injection tank 200 are connected by a circulating water pipe to form a circulating water circuit. The temperature control module is also set on the circulating water circuit to adjust the temperature of the water in the circulating water circuit. In some cases, the temperature control module can also be set at the water injection tank 200 to adjust the water temperature of the water injection tank 200, and then drive the circulating water pump 300 to make the water flow at the appropriate temperature flow to the water tank 100. It is understandable that Figure 1 This is the structural block diagram of the zebrafish anesthesia imaging auxiliary system of this application, which shows the connection relationship between each component through the circulating water pipe. Figure 2 This is a partial structural diagram of another embodiment of the zebrafish anesthesia imaging auxiliary system of the present application. The figure shows the position structure of other components except the water tank, the front temperature sensor and the rear temperature sensor. It should be noted that: Figure 2 The figure only provides a reference scheme for the location and structure of each module. The actual location of each module is not limited to the scheme in the figure. Figure 2 The specific piping connection relationship of each part is not shown in the figure. Figure 1 The scheme in the figure can be connected and adaptively adjusted according to actual conditions.

[0069] The water tank 100 is used to hold the zebrafish to be tested. The fixing bracket 150 within the water tank 100 secures the zebrafish to be tested. Imaging the zebrafish requires simply placing the water tank 100 in a designated location on the imaging device. In some cases, the water tank 100 is a horizontal cylindrical tube, specifically comprising a tube body and a tube cap, which are fixedly connected by threads thereon. The auxiliary port 140 is provided on the top wall of the tube body.

[0070] The water injection tank 200 is used to inject water. The water in the tank will be pressurized by the circulating water pump 300 and then flow through the water tank 100. It can be understood that the amount of water in the water injection tank 200 needs to ensure that the water tank 100 is full of water so that the zebrafish can survive normally. In some cases, the water injection tank 200 can be used to add anesthetic drugs or oxygen.

[0071] The auxiliary port 140 is used to adjust the body position of the zebrafish to be tested. Specifically, air can be injected into the water tank 100 through the auxiliary port 140 to change the water level and adjust the body position of the zebrafish by pressure.

[0072] The imaging principle of nuclear magnetic resonance (NMR) is based on changes in the magnetic field, which in turn cause changes in relaxation signals in small animals, and images are formed based on these changes. Metal fittings or sensors in the liquid pipelines of conventional equipment cannot be used in the NMR environment. Therefore, equipment suitable for NMR imaging needs to fully consider the special requirements of the NMR environment, including magnetic field compatibility, signal interference, and other issues. By selecting appropriate materials and optimizing pipeline design, the impact of pipelines on NMR imaging can be reduced. Specifically, it is generally believed that the area within two meters around the NMR machine is a strong magnetic area. The use of other metal fittings and sensors should be avoided in this strong magnetic area to prevent the strong magnetic area of ​​the NMR equipment from affecting the accuracy of the device.

[0073] Therefore, in the present application, the water tank 100 is made of non-metallic materials. When the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the water injection tank 200, the circulating water pump 300, the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800 are all set away from the magnetic resonance imaging equipment, and the water tank 100 is set within the detection range of the magnetic resonance imaging equipment. After such material design and position design, the equipment of the present application can be suitable for magnetic resonance imaging.

[0074] Because when performing nuclear magnetic resonance imaging, the water injection tank 200, the circulating water pump 300, the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800 are all set away from the nuclear magnetic resonance equipment, that is, they are also far away from the water tank 100. Therefore, there is a temperature difference between the water temperature in the water injection tank 200 and the water temperature in the water tank 100, and sensors cannot be installed near the water tank 100. In order to achieve the effect of precise control of water flow after long-distance transportation, the present application measures the water flow temperature at the inlet and outlet of the water tank 100 respectively, which can ensure the accuracy of temperature measurement without affecting nuclear magnetic resonance imaging and help to ensure the accuracy of control by the temperature control module.

[0075] The above-mentioned front temperature sensor 400 can be set at the circulating water pipe near the water inlet 120 of the water tank 100, and is used to collect the inlet water temperature, that is, the temperature of the water flowing into the water tank 100; the above-mentioned rear temperature sensor 500 can be set at the circulating water pipe near the water outlet 130 of the water tank 100, and is used to collect the outlet water temperature, that is, the temperature of the water flowing out of the water tank 100.

[0076] The control module 800 may be a single chip microcomputer; the control module 800 may also be a display control module, including a display unit 810 and a control unit. The display unit 810 may be a display, and the control unit may be a single chip microcomputer.

[0077] In some embodiments, the transparent plastic material used in the water tank 100 can be a 3D printed transparent PETG material or acrylic material, which ensures that the material is non-metallic and transparent for easy observation. The circulating water pipe uses a silicone tube.

[0078] In some embodiments, the zebrafish anesthesia imaging assistance system further comprises:

[0079] Box 900; water injection tank 200, circulating water pump 300, front temperature sensor 400, rear temperature sensor 500, temperature control module and control module 800 are all disposed in box 900;

[0080] In the case where the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the box 900 is set away from the magnetic resonance imaging equipment.

[0081] In this embodiment, the system structure is optimized by integrating the water injection tank 200, the circulating water pump 300, the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800 into the box 900. By placing the electrical part of the entire equipment pipeline inside the box 900 outside the nuclear magnetic resonance equipment, while meeting the fixing requirements, the use of metal materials in the strong magnetic area is avoided, and the equipment is prevented from being interfered with by the magnetic field, ensuring that all components in the mold can be used in a magnetic resonance environment.

[0082] In some embodiments, the front temperature sensor 400 and the rear temperature sensor 500 can be set on the pipeline outside the box 900, but still need to be set away from the nuclear magnetic resonance equipment. The box 900 is provided with a water injection tank 200, a circulating water pump 300, a temperature control module and a control module 800. Figure 2 shown.

[0083] In some embodiments, when the zebrafish anesthesia imaging assistance system is used for magnetic resonance imaging, the water tank 100 is set within the detection range of the magnetic resonance imaging device.

[0084] In this embodiment, the water tank 100 is set within the detection range of the nuclear magnetic resonance equipment, and nuclear magnetic resonance imaging can be performed on the zebrafish to be tested fixed in the water tank 100. Specifically, the water tank 100 is set inside the nuclear magnetic resonance coil of the nuclear magnetic resonance equipment.

[0085] In some embodiments, the MRI coil used in conjunction with the experimental zebrafish for MRI imaging can be a MRI small animal coil suitable for MRI of small animals. Specifically, a coil suitable for MRI of mice can be used. The inner diameter of the MRI small animal coil is generally about three centimeters. The maximum size of the water tank 100 used to place the zebrafish needs to be designed based on the inner diameter of the MRI small animal coil. It needs to be miniaturized so that the water tank 100 can be placed inside the MRI small animal coil to ensure the survival of the zebrafish while ensuring that the relaxation signal can be collected to achieve satisfactory imaging accuracy. In some cases, the size of the water tank 100 used to place the zebrafish is about seven millimeters. The size is designed based on the body size of an adult zebrafish, and only one adult zebrafish can be placed in one water tank 100, and it can be completely placed inside the MRI small animal coil.

[0086] In some embodiments, there are multiple water tanks 100, which are connected in series through the water inlet 120 and the water outlet 130. After the multiple water tanks 100 are connected in series, magnetic resonance imaging can be performed on the zebrafish to be tested in the multiple water tanks 100 at the same time, thereby improving experimental efficiency.

[0087] In some embodiments, the water tank 100 further comprises:

[0088] The partition 160 is used to separate the water tank 100 into an upper chamber and a lower chamber. The fixing bracket 150 is arranged in the upper chamber. The partition 160 is provided with a water hole 161.

[0089] In this embodiment, the water tank 100 is separated by setting a partition 160, and the fixing bracket 150 is set in the upper chamber, which can facilitate the fixing of the zebrafish to be tested in the middle of the water tank 100. When air is injected into the water tank 100 through the auxiliary port 140, the air will be above the upper chamber because it is lighter than water. The lower chamber at the bottom of the water tank 100 is used for water flow to pass through, which can change the water level and thus adjust the body position of the zebrafish; water holes 161 are set on the partition 160 because the zebrafish itself will occupy a certain volume and will separate the upper chamber into multiple spaces. There may be a place full of air and water cannot flow in. The water holes 161 on the partition 160 can act as a communicating vessel, introducing the water flow in the lower chamber into the separated spaces in the upper chamber. It can be understood that there are multiple water holes 161, such as Figure 2 and Figure 3As shown, the partition 160 is provided with nine water holes 161 in three rows and three columns.

[0090] In some embodiments, the fixed support 150 includes a first adjustable support frame and a second adjustable support frame, and the zebrafish to be tested are placed on the first adjustable support frame and the second adjustable support frame, and the first adjustable support frame and the second adjustable support frame are respectively used to adjust the height of both sides of the zebrafish to be tested.

[0091] In this embodiment, the two adjustable supports facilitate control of the swing amplitude of the zebrafish's body to the left and right, and the adjustable supports provide greater flexibility. In some cases, the first adjustable support and the second adjustable support are composed of two slopes, forming an inverted "V" shape. The first adjustable support and the second adjustable support together form an "M" shape, with the zebrafish positioned and fixed in the middle. In some cases, the two adjustable supports can be manually adjusted.

[0092] In some embodiments, the temperature control module includes a heating unit and a cooling unit, both of which are electrically connected to the control module 800 .

[0093] In this embodiment, the heating unit can increase the temperature of the water flow in the system, and the cooling unit can reduce the temperature of the water flow in the system. Both the heating unit and the cooling unit are controlled by the temperature control module.

[0094] In some embodiments, the heating unit is a section of instant hot water pipe 610, which can be equipped with an instant heating wire. The cooling unit is a section of cooling water pipe 620, which can be equipped with semiconductor cooling fins 621, heat dissipation fins 622, and a cooling fan 623. In some cases, a dry-boil prevention temperature sensor 611 is also provided within the housing 900. Specifically, the dry-boil prevention temperature sensor 611 can be provided near the instant hot water pipe 610 to protect the device. The instant hot water pipe 610 and the cooling water pipe 620 can be connected in series or in parallel to the circulating water pipe within the system.

[0095] In some embodiments, the zebrafish anesthesia imaging assistance system further includes a high temperature sensor 710 and a low temperature sensor 720, which are used for high temperature detection and low temperature detection, respectively. In some cases, the high temperature sensor 710 and the low temperature sensor 720 can be set in front of the water inlet 120, and an alarm is triggered when the measured water temperature is higher than a preset high temperature threshold or lower than a preset low temperature threshold, thereby ensuring the survival conditions of the zebrafish; in some cases, the high temperature sensor 710 and the low temperature sensor 720 can be respectively set near the hot water pipe 610 and the cooling water pipe 620, such as Figure 2 As shown, when the temperature near the device is measured to be too high or too low, an alarm is triggered to ensure the safety of the system device.

[0096] In some embodiments, the water injection tank 200 may have a removable cover, and in some cases, it may also have an opening that can be used for sampling. A valve may be provided at the opening, specifically, a normally closed solenoid valve. Valves may also be provided at the output port of the water injection tank 200 connected to the water inlet 120, and at the input port connected to the water outlet 130, specifically, a normally open solenoid valve.

[0097] In some embodiments, the zebrafish anesthesia imaging assistance system further includes a regulating water tank 210, which is connected to the water injection tank 200 via a pipeline. A valve, specifically a normally closed solenoid valve, may be provided on the pipeline. To adjust the liquid in the water injection tank 200, the regulating liquid is first injected into the regulating water tank 210, and then gradually injected into the water injection tank 200 through valve control. The regulating water tank 210 may have a removable cover.

[0098] In some embodiments, the auxiliary port 140 provided on the water tank 100 can be used as a metabolite collection port. During the imaging process of the zebrafish, metabolite sampling is performed through the auxiliary port 140 to collect information on substances such as metabolites and oxygen content in the water surrounding the zebrafish, and to observe the metabolic changes of the zebrafish during the entire imaging process, such as the composition of the excretion volume and oxygen content, so as to conduct further research and analysis in conjunction with the imaging results.

[0099] The present application also provides a zebrafish anesthesia imaging assistance method, which is applied to the zebrafish anesthesia imaging assistance system of the above embodiment. The zebrafish anesthesia imaging assistance method includes:

[0100] Obtain the inlet and outlet water temperatures of the water tank 100. The water injection tank 200, circulating water pump 300, front temperature sensor 400, rear temperature sensor 500, temperature control module, and control module 800 are all pre-installed away from the nuclear magnetic resonance equipment.

[0101] According to the inlet water temperature and the outlet water temperature, the current predicted water temperature in the water tank 100 is obtained, and the water temperature of the water flowing through the water tank 100 is adjusted so that the water temperature in the water tank 100 is within a preset temperature range;

[0102] When the current predicted water flow temperature in the cabin is within the preset temperature range, the anesthesia imaging operation is performed.

[0103] In the embodiment of the present application, by reasonably arranging the positions of each module and using a water tank 100 made of non-metallic material to place the zebrafish to be tested, the zebrafish anesthesia imaging assistance system of the present application can be applied to magnetic resonance imaging of zebrafish, solving the problem that the existing zebrafish imaging device is difficult to obtain good imaging effects and cannot clearly display the changes in the zebrafish body under the magnetic resonance environment; through the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800, and in conjunction with the zebrafish anesthesia imaging assistance method, high-precision water temperature control in the water tank 100 can be achieved, thereby achieving anesthesia assistance for the zebrafish, ensuring the safety of the zebrafish during the anesthesia process, and ensuring the stability of the imaging process; therefore, the present application provides an efficient and safe technical platform for in vivo biomedical research.

[0104] Tricaine is currently the mainstream anesthesia drug for zebrafish, while alternatives such as clove oil, etomidate, and propofol combined with lidocaine are also commonly used. However, these techniques still have significant drawbacks. Tricaine may induce active avoidance behavior in zebrafish, suggesting that it causes stress discomfort. Although etomidate has little interference with anxiety tests, long-term exposure can induce increased aggression, oxidative stress, and neurological damage. Clove oil carries the risk of dose-dependent toxicity, and individual variability makes standardization of anesthetic effects difficult. Therefore, reducing the anesthetic dose and simultaneously using a control device to adjust the water temperature can not only effectively avoid adverse drug reactions but also facilitate the control of zebrafish's recovery time from anesthesia through precise water temperature regulation.

[0105] For short-term imaging, simply controlling the water temperature can achieve stable imaging results. For example, when the water temperature is controlled at around 17°C, the zebrafish's activity is reduced. Furthermore, the fixed bracket 150 in the water chamber 100 further restricts the fish's activity, ensuring that anesthetics are not required for short-term imaging, such as within 10 to 20 minutes.

[0106] For imaging sessions longer than 30 minutes, this method significantly reduces the required concentration of the zebrafish anesthetic MS-222 by precisely regulating the water temperature, while ensuring effective anesthesia and preventing any impact on the imaging process. Specifically, while the typical anesthetic concentration is 40 mg / L, by controlling the water temperature within a preset range, the anesthetic concentration can be further reduced to 20 mg / L or even 10 mg / L.

[0107] The above-mentioned inlet water temperature can be measured by the front temperature sensor 400, and the above-mentioned outlet water temperature can be measured by the rear temperature sensor 500. Since, in the case of nuclear magnetic resonance imaging, the water injection tank 200, the circulating water pump 300, the front temperature sensor 400, the rear temperature sensor 500, the temperature control module and the control module 800 are all arranged far away from the nuclear magnetic resonance equipment, that is, they are also far away from the water tank 100. Therefore, there is a temperature difference between the water temperature in the water injection tank 200 and the water temperature in the water tank 100, and sensors cannot be installed near the water tank 100. In order to achieve the effect of precise control of water flow after long-distance transportation, the present application measures the water flow temperature at the inlet and outlet of the water tank 100 respectively. This can ensure the accuracy of temperature measurement without affecting nuclear magnetic resonance imaging and help ensure the accuracy of control by the temperature control module.

[0108] In some embodiments, obtaining the current predicted water temperature in the water tank 100 based on the inlet water temperature and the outlet water temperature includes:

[0109] According to a preset weighting ratio, the inlet water temperature and the outlet water temperature are weightedly calculated to obtain the current predicted water temperature in the water tank 100; wherein, the preset weighting ratio is obtained by performing a linear regression analysis on the inlet water temperature, the outlet water temperature and the actual water tank water temperature when the nuclear magnetic resonance equipment is turned off. The actual water tank water temperature can be measured by setting a temperature sensor at the auxiliary port 140 of the water tank 100.

[0110] The above preset weighted ratio is obtained by linear regression analysis of the inlet water temperature, outlet water temperature and actual water tank water temperature, which is the optimal weighted ratio and can accurately predict the actual temperature in the tank.

[0111] In some embodiments, the inlet water temperature and the outlet water temperature are weighted according to a preset weighting ratio to obtain the current predicted water temperature in the water tank 100, which is constrained by the following expression:

[0112] CurrentTemp=temp1*0.44+temp2*0.56;

[0113] Among them, CurrentTemp is the predicted water temperature in the cabin, temp1 is the inlet water temperature, and temp2 is the outlet water temperature.

[0114] In some embodiments, obtaining a current predicted water temperature in the water tank 100 based on the inlet water temperature and the outlet water temperature, and adjusting the temperature of the water flowing through the water tank 100 so that the water temperature in the water tank 100 is within a preset temperature range, includes:

[0115] When the water temperature in the water tank is predicted to be higher than a preset high temperature threshold or lower than a preset low temperature threshold, the circulating water pump 300 is turned off, and the temperature control module is controlled to operate to adjust the water temperature in the water tank 100 so that the water temperature in the water tank 100 tends to a preset temperature range; the preset low temperature threshold is lower than the lower threshold value of the preset temperature range, and the preset high temperature threshold is higher than the upper threshold value of the preset temperature range;

[0116] When the water temperature in the predicted cabin exceeds the preset temperature range and is within the preset low temperature threshold and the preset high temperature threshold constraint range, the temperature control module is controlled to operate to adjust the water temperature in the water tank 100 so that the water temperature in the water tank 100 tends to the preset temperature range.

[0117] In this embodiment, to maximize the protection of the precious experimental subjects, preset low and high temperature thresholds are set to prevent extremely high or low temperatures that could affect the survival of the zebrafish. When the thresholds are exceeded, the circulating water pump 300 is shut down to reduce water flow at abnormal water temperatures. In some cases, the preset low temperature threshold is 10°C, and the preset high temperature threshold is 30°C.

[0118] In some embodiments, the temperature sensor's minimum measurement value is 0.1°C, so the feedback accuracy is set to 0.1°C. In practice, the temperature will fluctuate slightly within 0.5°C, affected by the distance between the water pump and the experimental water chamber. After setting the preset temperature range, the water temperature is precisely adjusted to maintain a ±0.5°C error, ensuring the safety of the zebrafish during anesthesia. During operation, water temperature fluctuations should be closely monitored, and the temperature control system parameters should be adjusted accordingly to maintain water temperature stability.

[0119] In some embodiments, when the current predicted water flow temperature in the chamber is within a preset temperature range, performing an anesthesia imaging operation includes:

[0120] When the current predicted water temperature in the water tank is within the preset temperature range, the zebrafish to be tested is placed in the water tank 100;

[0121] Adjust the fixing bracket 150 to fix the zebrafish to be tested;

[0122] Inject air into the water chamber 100 through the auxiliary port 140 to adjust the zebrafish to the position required for imaging;

[0123] The water tank 100 is placed within the detection range of the nuclear magnetic resonance device to perform an anesthesia imaging operation.

[0124] In this embodiment, after the water temperature is adjusted to a preset temperature range suitable for the survival of zebrafish and capable of reducing the activity of zebrafish, the zebrafish to be tested is placed in the water tank 100, and then the zebrafish to be tested is adjusted and fixed so that it is in the body position required for imaging. The water tank 100 is then placed in the MRI coil of the MRI device. If it is a short-term imaging, the entire system controls the activity state of the zebrafish through low temperature, and no external anesthetic drugs are required. The MRI device can be directly operated to start imaging. If the imaging time exceeds half an hour or even longer, anesthetic drugs can be added by filling the water tank 200 or adjusting the water tank 210 for anesthesia, and then the MRI device is operated to start imaging.

[0125] In some embodiments, through experimental verification, the present application has achieved high-definition dynamic imaging of deep tissues of zebrafish under MRI environment through the innovative design of a magnetic resonance-compatible zebrafish anesthesia imaging auxiliary system, with a resolution of 0.5mm, and achieved precise control of anesthesia time, with a recovery time error of ≤3 minutes, and achieved significant improvement in experimental stability, with continuous imaging for more than 30 minutes, solving the current technical gap in the long-term high-definition magnetic resonance imaging of zebrafish. At the same time, multi-parameter detection of the activity status of zebrafish provides an efficient and safe interdisciplinary technology platform for in vivo biomedical research.

[0126] In addition, an embodiment of the present invention further provides a control device, which includes: a memory, a processor, and a computer program stored in the memory and executable on the processor. The processor and the memory may be connected via a bus or other means.

[0127] The memory, as a non-transient computer-readable storage medium, can be used to store non-transient software programs and non-transient computer executable programs. In addition, the memory may include a high-speed random access memory and may also include a non-transient memory, such as at least one disk storage device, a flash memory device, or other non-transient solid-state storage device. In some embodiments, the memory may optionally include a memory remotely arranged relative to the processor, and these remote memories may be connected to the processor via a network. Examples of the above-mentioned network include, but are not limited to, the Internet, an intranet, a local area network, a mobile communication network, and combinations thereof.

[0128] The non-transient software program and instructions required to implement the zebrafish anesthesia imaging assistance method of the above embodiment are stored in the memory, and when executed by the processor, the zebrafish anesthesia imaging assistance method of the above embodiment is executed.

[0129] The device embodiments described above are merely illustrative. The units described as separate components may or may not be physically separate, i.e., they may be located in one place or distributed across multiple network units. Some or all of the modules may be selected based on actual needs to achieve the objectives of this embodiment.

[0130] In addition, an embodiment of the present invention also provides a computer-readable storage medium, which stores computer-executable instructions. The computer-executable instructions are executed by a processor or controller, for example, by a processor of the above-mentioned control device, so that the above-mentioned processor can execute the zebrafish anesthesia imaging auxiliary method in the above-mentioned embodiment.

[0131] Those skilled in the art will appreciate that all or some of the steps and systems in the method disclosed above can be implemented as software, firmware, hardware, and appropriate combinations thereof. Some physical components or all physical components can be implemented as software executed by a processor, such as a central processing unit, a digital signal processor, or a microprocessor, or implemented as hardware, or implemented as an integrated circuit, such as an application-specific integrated circuit. Such software can be distributed on a computer-readable medium, and the computer-readable medium can include computer storage media (or non-transitory media) and communication media (or temporary media). As known to those skilled in the art, the term computer storage media is included in any method or technology for storing information (such as computer-readable instructions, data structures, program modules, or other data) and is volatile and non-volatile, removable, and non-removable. Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory, or other memory technology, CD-ROM, digital versatile disks (DVD), or other optical disk storage, magnetic cassettes, magnetic tapes, disk storage, or other magnetic storage devices, or any other medium that can be used to store desired information and can be accessed by a computer. Furthermore, as is well known to those skilled in the art, communication media typically embodies computer-readable instructions, data structures, program modules, or other data in a modulated data signal such as a carrier wave or other transport mechanism, and may include any information delivery media.

[0132] Throughout this specification, reference to terms such as "one embodiment," "some implementations," "illustrative embodiments," "examples," "specific examples," or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0133] Although the embodiments of the present application are described in detail above in conjunction with the accompanying drawings, the present application is not limited to the above embodiments. Those skilled in the art will understand that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and purpose of the present application. The scope of the present application is defined by the claims and their equivalents.

Claims

1. A zebrafish anesthesia imaging assistance system, characterized in that: include: A water tank having a cover for sealing the water tank; a water inlet is provided on the cover, and an auxiliary port and a water outlet are provided on the water tank, wherein the water outlet is located at an end of the water tank away from the cover; a fixing bracket is provided in the water tank, wherein the fixing bracket is used to fix the zebrafish to be tested, and the auxiliary port is used to adjust the body position of the zebrafish to be tested; the water tank is made of non-metallic material; a water injection tank having an output port in communication with the water inlet and an input port in communication with the water outlet; a circulating water pump, for circulating water between the water tank and the water injection tank; A front temperature sensor, used to collect the inlet water temperature of the water inlet; A rear temperature sensor is used to collect the outlet water temperature of the water outlet; a temperature control module, for regulating the temperature of water flowing through the water tank and the water injection tank; a control module, electrically connected to the front temperature sensor, the rear temperature sensor, and the temperature control module, and configured to control the temperature control module to adjust the water temperature inside the water tank according to the inlet water temperature and the outlet water temperature, so as to maintain the anesthesia state of the zebrafish to be tested; Among them, when the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all arranged away from the magnetic resonance imaging equipment.

2. The zebrafish anesthesia imaging assistance system according to claim 1, characterized in that: Also includes: The water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all arranged in the box; Wherein, when the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the box is arranged away from the magnetic resonance imaging device.

3. The zebrafish anesthesia imaging assistance system according to claim 1, characterized in that: When the zebrafish anesthesia imaging auxiliary system is used for magnetic resonance imaging, the water tank is set within the detection range of the magnetic resonance imaging device.

4. The zebrafish anesthesia imaging assistance system according to claim 1, characterized in that: There are multiple water tanks, and the multiple water tanks are connected in series in sequence through the water inlet and the water outlet.

5. The zebrafish anesthesia imaging assistance system according to claim 1, characterized in that: The water tank also includes: The partition is used to separate the water tank into an upper chamber and a lower chamber. The fixing bracket is arranged in the upper chamber. The partition is provided with a water hole.

6. The zebrafish anesthesia imaging assistance system according to claim 1 or 5, characterized in that: The fixed support includes a first adjustable support frame and a second adjustable support frame. The zebrafish to be tested is placed on the first adjustable support frame and the second adjustable support frame. The first adjustable support frame and the second adjustable support frame are respectively used to adjust the height of both sides of the zebrafish to be tested.

7. The zebrafish anesthesia imaging assistance system according to claim 1, characterized in that: The temperature control module includes a heating unit and a cooling unit both electrically connected to the control module.

8. A zebrafish anesthesia imaging assistance method, applied to the zebrafish anesthesia imaging assistance system according to any one of claims 1 to 7, characterized in that: The following steps are involved: Obtaining the inlet water temperature and the outlet water temperature of the water tank, wherein the water injection tank, the circulating water pump, the front temperature sensor, the rear temperature sensor, the temperature control module and the control module are all pre-installed away from the nuclear magnetic resonance equipment; Obtaining a current predicted water temperature in the water tank according to the inlet water temperature and the outlet water temperature, and adjusting the temperature of the water flowing through the water tank so that the water temperature in the water tank is within a preset temperature range; When the current predicted water flow temperature in the cabin is within the preset temperature range, an anesthesia imaging operation is performed.

9. The zebrafish anesthesia imaging assisting method according to claim 8, characterized in that: The step of obtaining a current predicted water temperature in the water tank according to the inlet water temperature and the outlet water temperature, and adjusting the temperature of the water flowing through the water tank so that the water temperature in the water tank is within a preset temperature range, includes: When the predicted water temperature in the water tank is higher than a preset high temperature threshold or lower than a preset low temperature threshold, the circulating water pump is turned off, and the temperature control module is controlled to operate so as to adjust the water temperature in the water tank so that the water temperature in the water tank tends to the preset temperature range; the preset low temperature threshold is lower than the lower threshold value of the preset temperature range, and the preset high temperature threshold is higher than the upper threshold value of the preset temperature range; When the predicted water temperature in the water tank exceeds the preset temperature range and is within the constraint range of the preset low temperature threshold and the preset high temperature threshold, the temperature control module is controlled to operate to adjust the water temperature in the water tank so that the water temperature in the water tank tends to the preset temperature range.

10. The zebrafish anesthesia imaging auxiliary method according to claim 8, characterized in that: The performing of the anesthesia imaging operation when the current predicted water flow temperature in the cabin is within the preset temperature range includes: When the current predicted water flow temperature in the water tank is within the preset temperature range, placing the zebrafish to be tested into the water tank; Adjusting the fixing bracket to fix the zebrafish to be tested; Injecting air into the water tank through the auxiliary port to adjust the zebrafish to a body position required for imaging; The water tank is placed within the detection range of the nuclear magnetic resonance device to perform an anesthesia imaging operation.

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