Poultry immunosuppressive disease prevention and treatment preparation based on rhizoma atractylodis macrocephalae compound and application thereof

Abstract

The invention belongs to the technical field of poultry disease prevention and treatment pharmaceutical agents, and discloses a poultry immunosuppressive disease prevention and treatment preparation based on a rhizoma atractylodis macrocephalae compound and application thereof, and the preparation is prepared from the following raw materials in parts by weight: 90-150 parts of rhizoma atractylodis macrocephalae, 60-100 parts of codonopsis pilosula, 60-100 parts of poria cocos, 30-50 parts of honey-fried licorice root, 30-50 parts of dandelion and 30-50 parts of purslane. By adjusting the immune system of the goslings, the disease resistance of the goslings is enhanced, and the occurrence rate of epidemic diseases is reduced, so that the breeding cost is reduced, and the economic benefit is improved; meanwhile, the use of the traditional Chinese medicine compound preparation can reduce the use of antibiotics and reduce the risk of drug resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of pharmaceutical preparations for preventing and treating poultry diseases, and in particular to a poultry immunosuppressive disease prevention and treatment preparation based on a compound of Atractylodes macrocephala and an application thereof. Background Art

[0002] With the rapid development of large-scale, intensive gosling farming, frequent disease outbreaks have become a major concern for the industry. Goslings' immune systems are not fully developed in their early stages, making them vulnerable to a variety of pathogens. While traditional Western antibiotics can quickly suppress pathogens, long-term use can easily lead to the development of drug resistance in pathogens, and antibiotic residues pose a potential threat to food safety and the ecological environment. Furthermore, while vaccination can prevent disease to a certain extent, due to the rapid mutation of pathogens, the protective effectiveness of existing vaccines often fails to keep pace with the evolution of pathogens, leading to a disconnect between production and application.

[0003] In recent years, the application of Chinese herbal compound in livestock and poultry farming has gradually attracted attention. Compared with single-ingredient Western medicine, Chinese herbal compound can effectively reduce the risk of drug resistance through the synergistic effect of multiple active ingredients, while reducing the potential harm of drug residues to the environment and human body.

[0004] Therefore, there is an urgent need to develop a Chinese herbal compound for the prevention and treatment of poultry immunosuppressive diseases. Summary of the Invention

[0005] The present invention aims to provide a poultry immunosuppressive disease prevention and treatment preparation based on a compound of Atractylodes macrocephala and its application. The preparation regulates the immune system of goslings, enhances their disease resistance, reduces the incidence of epidemic diseases, thereby reducing breeding costs and improving economic benefits. At the same time, the use of the Chinese medicine compound preparation can reduce the use of antibiotics and reduce the risk of drug resistance, which is in line with the development direction of modern green breeding.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A preparation for preventing and treating poultry immunosuppressive diseases based on a compound of Atractylodes macrocephala is prepared from the following raw materials in parts by weight: 90-150 parts of Atractylodes macrocephala, 60-100 parts of Codonopsis pilosula, 60-100 parts of Poria cocos, 30-50 parts of roasted Licorice root, 30-50 parts of Taraxacum mongolicum, and 30-50 parts of Portulaca oleracea.

[0008] Furthermore, it is prepared from the following raw materials in parts by weight: 90 parts of Atractylodes macrocephala, 60 parts of Codonopsis pilosula, 60 parts of Poria cocos, 30 parts of roasted Licorice root, 30 parts of Taraxacum mongolicum, and 30 parts of Portulaca oleracea.

[0009] Furthermore, it is prepared from the following raw materials in parts by weight: 120 parts of Atractylodes macrocephala, 80 parts of Codonopsis pilosula, 80 parts of Poria cocos, 40 parts of roasted Licorice root, 40 parts of Taraxacum mongolicum, and 40 parts of Portulaca oleracea.

[0010] Furthermore, it is prepared from the following raw materials in parts by weight: 150 parts of Atractylodes macrocephala, 100 parts of Codonopsis pilosula, 100 parts of Poria cocos, 50 parts of roasted Licorice root, 50 parts of Taraxacum mongolicum, and 50 parts of Portulaca oleracea.

[0011] According to the above application of the poultry immunosuppressive disease prevention and treatment preparation based on the Atractylodes macrocephala compound in geese.

[0012] The principle and beneficial effects of the technical solution are:

[0013] The Chinese medicine compound preparation of the present invention can enhance the disease resistance of goslings by regulating their immune system, reduce the incidence of epidemic diseases, thereby reducing breeding costs and improving economic benefits; at the same time, the use of Chinese medicine compound can reduce the use of antibiotics, reduce the risk of drug resistance, and conform to the development direction of modern green breeding. Compared with Western medicine with a single ingredient, Chinese medicine compound can effectively reduce the risk of drug resistance through the synergistic effect of multiple active ingredients, while reducing the potential harm of drug residues to the environment and human body. Specifically, components such as Atractylodes macrocephala, Poria cocos, and Licorice have the effects of replenishing qi and strengthening the spleen, and enhancing the body's immunity. Studies have shown that the active ingredients such as polysaccharides and flavonoids of components such as Atractylodes macrocephala, Poria cocos, and Licorice can significantly improve the body's immune function and promote the proliferation and activation of immune cells. On this basis, medicinal materials such as dandelion and purslane are added to further enhance the effects of clearing away heat and detoxifying, reducing swelling and dispersing stagnation, and stopping diarrhea and bleeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a bar graph showing the regulatory effect of CHC on the immune function and antioxidant function of goslings induced by cyclophosphamide in Example 4 of the present invention; in the figure, A is a bar graph showing the results of ELISA detection of serum IgA, IgG, IgM, IGF-1 and IL-10 levels in goslings and their immune function evaluation; B is a bar graph showing the results of detection of SOD, T-AOC, GSH-Px, MDA and iNOS in the bursa of Fabricius;

[0015] Figure 2 This is a histogram showing the regulatory effect of CHC on CTX-induced liver steatosis in goslings in Example 4 of the present invention. In the figure, A is a histogram showing the biochemical test results related to serum lipids and liver function, and B is a histogram showing the antioxidant index and blood lipid test results of liver tissue.

[0016] Figure 3 This is a bar graph showing the regulatory effect of CHC on CTX-induced liver and kidney function in goslings in Example 4 of the present invention; in the figure, A is a bar graph of the detection results of SOD, GSH-Px, and MDA in the liver, B is a bar graph of the detection results of SOD, GSH-Px, and MDA in the kidney, and C is a bar graph of the detection results of UA, BUN, and K+ in serum. DETAILED DESCRIPTION

[0017] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments:

[0018] Example 1

[0019] A preparation for preventing and treating poultry immunosuppressive diseases based on a compound of Atractylodes macrocephala is prepared from the following raw materials by weight: 90g of Atractylodes macrocephala, 60g of Codonopsis pilosula, 60g of Poria cocos, 30g of roasted Licorice root, 30g of Taraxacum mongolicum, and 30g of Portulaca oleracea.

[0020] The preparation of feed based on the above-mentioned control preparation comprises the following steps:

[0021] Step 1: Weigh 90g of Atractylodes macrocephala, 60g of Codonopsis pilosula, 60g of Poria cocos, 30g of Radix Glycyrrhizae, 30g of Taraxacum mongolicum, and 30g of Portulaca oleracea, and mix all the Chinese medicine powders evenly;

[0022] Step 2: Put the Chinese medicine powder into a gauze bag and put it into a casserole. Add water 3 times the weight of the medicine into the casserole and soak for 30 minutes. Decoction twice. The first decoction is 45 minutes. After decoction, filter with a 100-mesh nylon sieve. Decoction the second time for 30 minutes. After filtering, combine the two decoctions and concentrate to 1g / mL for later use.

[0023] Example 2

[0024] A preparation for preventing and treating poultry immunosuppressive diseases based on a compound of Atractylodes macrocephala is prepared from the following raw materials by weight: 120g of Atractylodes macrocephala, 80g of Codonopsis pilosula, 80g of Poria cocos, 40g of prepared Licorice root, 40g of Taraxacum mongolicum, and 40g of Portulaca oleracea.

[0025] The preparation of feed based on the above-mentioned control preparation comprises the following steps:

[0026] Step 1: Weigh 120g of Atractylodes macrocephala, 80g of Codonopsis pilosula, 80g of Poria cocos, 40g of Radix Glycyrrhizae Preparata, 40g of Taraxacum mongolicum, and 40g of Portulaca oleracea, and mix all the Chinese medicine powders evenly;

[0027] Step 2: Put the Chinese medicine powder into a gauze bag and put it into a casserole. Add water 3 times the weight of the medicine into the casserole and soak for 30 minutes. Decoction twice. The first decoction is 45 minutes. After decoction, filter with a 100-mesh nylon sieve. Decoction the second time for 30 minutes. After filtering, combine the two decoctions and concentrate to 1g / mL for later use.

[0028] Example 3

[0029] A preparation for preventing and treating poultry immunosuppressive diseases based on a compound of Atractylodes macrocephala is prepared from the following raw materials by weight: 150g of Atractylodes macrocephala, 100g of Codonopsis pilosula, 100g of Poria cocos, 50g of prepared Licorice root, 50g of Taraxacum mongolicum, and 50g of Portulaca oleracea.

[0030] The preparation of feed based on the above-mentioned control preparation comprises the following steps:

[0031] Step 1: Weigh 150g of Atractylodes macrocephala, 100g of Codonopsis pilosula, 100g of Poria cocos, 50g of Radix Glycyrrhizae, 50g of Taraxacum mongolicum, and 50g of Portulaca oleracea, and mix all the Chinese medicine powders evenly;

[0032] Step 2: Put the Chinese medicine powder into a gauze bag and put it into a casserole. Add water 3 times the weight of the medicine into the casserole and soak for 30 minutes. Decoction twice. The first decoction is 45 minutes. After decoction, filter with a 100-mesh nylon sieve. Decoction the second time for 30 minutes. After filtering, combine the two decoctions and concentrate to 1g / mL for later use.

[0033] Example 4

[0034] The following test was performed using the Chinese medicinal compound preparation prepared in Example 1:

[0035] 1. Experimental Animals

[0036] There are 48 one-day-old Magang goslings, half male and half female.

[0037] 2. Experimental Design

[0038] After three days of pre-feeding, goslings were randomly divided into four groups, each consisting of 12. There were no significant differences in body weight between the groups (P>0.05). The control and CTX groups were fed a conventional diet, while the CHC and CHC+CTX groups received a 2% compound Chinese herbal decoction (containing 1 mL / g of the raw herbs) added to their feed until the end of the experimental period. Goslings were weighed in the morning, fasting without food or water, at 19, 20, and 21 days of age. Goslings in the CTX and CHC+CTX groups received a 40 mg / kg body weight CTX solution injected into their leg muscles, while those in the control and CHC groups received a 0.5 mL saline injection into their leg muscles. Goslings in the morning, fasting without food or water, were weighed at 28 days of age. Goslings were handled according to animal welfare standards, and serum and organ samples were collected and stored for future use. The main experimental period lasted 24 days. During the breeding period, the technical specifications for the breeding and management of Magang goose meat geese (Guangdong Provincial Local Standard DB44 / T1595-2015) shall be strictly implemented.

[0039] 3. Related drugs

[0040] (1) Cyclophosphamide (CTX):

[0041] Drug approval number: National Medicine Standard HJ20160467, manufacturer: Baxter International GmbH (Germany).

[0042] (2) Compound Chinese medicine preparation: prepared as in Example 1.

[0043] 4. Experimental results

[0044] (1) Chinese herbal compound regulates the immune function and antioxidant capacity of goslings

[0045] In order to explore whether the Chinese herbal compound has immunomodulatory effects, immune-related factors in serum were detected. Figure 1 As shown in A, compared with the CHC group, the CTX group significantly reduced the levels of serum IgA, IL-10, and IGF-1 (P<0.05), and there was a certain downward trend for IgG (P>0.05). In addition, the effect of the Chinese herbal compound on the antioxidant function of the bursa of Fabricius was further explored. Figure 1 As shown in B, compared with the CHC group, the CTX group significantly reduced the activities of SOD, T-AOC, and GSH-Px (P<0.05), and significantly increased the levels of MDA and iNOS (P<0.05). Figure 1 In A and 1B, data columns marked with different letters indicate significant differences (P<0.05), and no letters or the same letters indicate no significant differences (P>0.05).

[0046] In summary, adding Chinese herbal compound to feed has a certain immunomodulatory effect, enhances the body's antioxidant capacity, and alleviates oxidative stress or inflammation caused by CTX.

[0047] (2) Regulatory effect of the Chinese herbal compound CTX on gosling liver steatosis

[0048] To explore the alleviating effect of traditional Chinese medicine compound on CTX-induced hepatic steatosis, the relevant indicators in serum and liver tissue were detected. Figure 2 As shown in A, compared with the CHC group, the CTX group significantly increased the serum T-BIL, LDL-C, and TC levels (P<0.05), indicating that CTX may have caused liver dysfunction and lipid metabolism disorders, while the CHC+CTX group could alleviate the liver function diseases caused by CTX to a certain extent, indicating that CHC can regulate CTX-induced liver steatosis. In addition, liver antioxidant and blood lipid indicators were detected. Figure 2 As shown in Figure B, compared with the CHC group, the CTX group significantly reduced the activity of GSH-Px and SOD (P<0.05), while the CHC+CTX group could effectively alleviate this phenomenon. In addition, compared with the Control group, the CTX group significantly increased the content of TG and TC in the liver (P<0.05), while the CHC+CTX group could significantly alleviate this phenomenon. Figure 2 In A and 2B, data columns marked with different letters indicate significant differences (P<0.05), and no letters or the same letters indicate no significant differences (P>0.05).

[0049] The results showed that adding Chinese herbal compound to feed could alleviate CTX-induced liver fatty degeneration.

[0050] (3) Regulatory effect of Chinese herbal compound on CTX-induced liver and kidney function in goslings

[0051] like Figure 3 As shown in A, compared with the CHC group, the CTX group significantly reduced the activities of GSH-Px and SOD in the liver (P<0.05), while the CHC+CTX group could effectively alleviate this phenomenon. Compared with the Control group, the CTX group significantly increased the content of MDA. Figure 3 As shown in B, compared with the CHC group, the CTX group significantly reduced the activity of GSH-Px in the kidney and significantly increased the content of MDA (P<0.05). In addition, the serum renal function indicators were further detected. Figure 3 As shown in C, compared with the CHC group, the CTX group significantly increased UA, BUN, and K+ levels (P<0.05). Figure 3 In A, 3B, and 3C, data columns marked with different letters indicate significant differences (P<0.05), and no letters or the same letters indicate no significant differences (P>0.05).

[0052] In summary, CTX causes a decrease in the antioxidant capacity of the liver and kidneys and may cause renal insufficiency, and the addition of Chinese herbal compound to feed can alleviate this phenomenon.

[0053] The Chinese medicine compound preparation of the present invention can enhance the disease resistance of goslings by regulating their immune system, reduce the incidence of epidemic diseases, thereby reducing breeding costs and improving economic benefits; at the same time, the use of Chinese medicine compound can reduce the use of antibiotics, reduce the risk of drug resistance, and conform to the development direction of modern green breeding. Compared with Western medicine with a single ingredient, Chinese medicine compound can effectively reduce the risk of drug resistance through the synergistic effect of multiple active ingredients, while reducing the potential harm of drug residues to the environment and human body. Specifically, components such as Atractylodes macrocephala, Poria cocos, and Licorice have the effects of replenishing qi and strengthening the spleen, and enhancing the body's immunity. Studies have shown that the active ingredients such as polysaccharides and flavonoids of components such as Atractylodes macrocephala, Poria cocos, and Licorice can significantly improve the body's immune function and promote the proliferation and activation of immune cells. On this basis, medicinal materials such as dandelion and purslane are added to further enhance the effects of clearing away heat and detoxifying, reducing swelling and dispersing stagnation, and stopping diarrhea and bleeding.

[0054] The above is only an embodiment of the present invention, and common knowledge such as the specific technical solutions or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several variations and improvements can be made, which should also be regarded as the scope of protection of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the description can be used to interpret the content of the claims.

Claims

1. A preparation for preventing and treating poultry immunosuppressive diseases based on a compound of Atractylodes macrocephala, characterized in that: The invention is prepared from the following raw materials in parts by weight: 90-150 parts of Atractylodes macrocephala, 60-100 parts of Codonopsis pilosula, 60-100 parts of Poria cocos, 30-50 parts of roasted Licorice root, 30-50 parts of Taraxacum mongolicum and 30-50 parts of Portulaca oleracea.

2. The preparation for preventing and treating poultry immunosuppressive diseases based on the Atractylodes macrocephala compound according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 90 parts of Atractylodes macrocephala, 60 parts of Codonopsis pilosula, 60 parts of Poria cocos, 30 parts of prepared Licorice root, 30 parts of Taraxacum mongolicum and 30 parts of Portulaca oleracea.

3. The poultry immunosuppressive disease prevention and treatment preparation based on the Atractylodes macrocephala compound according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 120 parts of Atractylodes macrocephala, 80 parts of Codonopsis pilosula, 80 parts of Poria cocos, 40 parts of prepared Licorice root, 40 parts of Taraxacum mongolicum and 40 parts of Portulaca oleracea.

4. The poultry immunosuppressive disease prevention and treatment preparation based on the Atractylodes macrocephala compound according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 150 parts of Atractylodes macrocephala, 100 parts of Codonopsis pilosula, 100 parts of Poria cocos, 50 parts of prepared Licorice root, 50 parts of Taraxacum mongolicum and 50 parts of Portulaca oleracea.

5. Use of the preparation for preventing and treating avian immunosuppressive diseases based on the Atractylodes macrocephala compound according to claim 1 in geese.

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