Wound generation dressing based on micro-nano technology and preparation method of wound generation dressing

Abstract

The invention discloses a wound-generating dressing based on a micro-nano technology and a preparation method thereof. The preparation method comprises the following steps: (1) weighing bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factors, fibronectin and a pH regulator in preset parts by weight; (2) adding deionized water and a pH regulator into fibronectin, then adding bioglass, hyaluronic acid and epidermal growth factors, and fully stirring to obtain a mixture 1; (3) heating vaseline and liquid paraffin to 60-80 DEG C to obtain a mixture 2; (4) adding the mixture 2 into the mixture 1, and uniformly stirring and mixing to obtain a cream dressing; and (5) dipping the nano reticular cellulose acetate fibers in the cream dressing, sterilizing and packaging to obtain the wound dressing. According to the invention, bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factors, fibronectin and a pH regulator are adopted as main raw materials, and the wound-generating dressing with good mechanical properties and antibacterial properties is prepared.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedical materials, and in particular relates to a biomedical dressing based on micro-nano technology and a preparation method thereof. Background Art

[0002] Skin, as the epidermis of the human body, protects the body's internal environment and maintains its stability. The ultimate goal of treating skin injuries is to restore the skin's barrier function. High-performance medical dressings can provide an environment conducive to wound healing, possessing excellent biocompatibility and anti-infection capabilities. By covering damaged skin, creating a wound healing environment and protecting the skin, they are a common medical device with broad prospects and research potential.

[0003] At present, most medical excipient products are in the form of films, sponges, gels, etc. Although these dressings have the advantages of hemostasis, coagulation, pain relief, and accelerated healing, the mechanical properties and antibacterial properties of these biological dressings still need to be further improved. Therefore, the development of a micro-nano bioinvasive dressing with excellent mechanical properties and excellent antibacterial effects is a technical problem that needs to be solved urgently. Summary of the Invention

[0004] In order to solve the deficiencies in the prior art, the present invention aims to provide a bio-injury dressing based on micro-nano technology and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: A method for preparing a bio-injury dressing based on micro-nanotechnology comprises the following steps: (1) Weigh the preset weight amounts of bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factor, fibronectin and pH regulator and set aside; (2) adding deionized water and a pH regulator to the fibronectin to adjust the pH value to a preset range, and then adding bioglass, hyaluronic acid, and epidermal growth factor and stirring thoroughly to obtain a mixture 1 for later use; (3) Heat vaseline and liquid paraffin to 60-80°C to obtain mixture 2, which is set aside; (4) adding the mixture 2 obtained in step (3) to the mixture 1 obtained in step (2), stirring the mixture by a stirring device to mix the mixture uniformly, and obtaining a creamy dressing; (5) The nano-mesh acetate fiber is impregnated into the creamy dressing obtained in step (4), and after impregnation for a certain period of time, the nano-mesh acetate fiber is sterilized and packaged to obtain a bio-injury dressing based on micro-nano technology.

[0006] Preferably, in step (1), the bioglass comprises 18-22 parts by weight, vaseline 20-30 parts by weight, liquid paraffin 12-18 parts by weight, hyaluronic acid 8-12 parts by weight, epidermal growth factor 5-8 parts by weight, fibronectin 10-18 parts by weight, and pH regulator 6-12 parts by weight.

[0007] Preferably, the pH regulator in step (1) is at least one of boric acid, acetic acid, phosphoric acid, triethanolamine, sodium hydroxide and potassium hydroxide.

[0008] Preferably, the preset range of pH value in step (2) is 3.5-4.5.

[0009] Preferably, the weight portion of deionized water added in step (2) is 80-120 weight portions.

[0010] Preferably, the stirring speed of the stirring device in step (4) is 1000-1200 r / min, and the stirring time is 20-30 min.

[0011] Preferably, the immersion time in step (5) is 12-24 hours.

[0012] Preferably, the sterilization in step (5) is carried out by ultraviolet irradiation.

[0013] Preferably, the packaging in step (5) is performed using a hose or a needle.

[0014] Preferably, a bio-injury dressing based on micro-nano technology is prepared by the above-mentioned preparation method.

[0015] Compared with the prior art, the present invention has the following positive and beneficial effects: The present invention uses bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factor, fibronectin and a pH regulator as main raw materials, prepares the above raw materials into a water-in-oil system creamy mixture according to a certain proportion, then impregnates the creamy mixture with nano-mesh acetate fiber, and undergoes ultraviolet sterilization and encapsulation to obtain a creamy bio-wound dressing based on micro-nano technology. The dressing has good mechanical properties and antibacterial properties. In addition, the raw materials used in the present invention are non-toxic and harmless, and have good biocompatibility and degradability. The preparation process is simple and the production cost is low. The prepared bio-wound dressing based on micro-nano technology has good application prospects. DETAILED DESCRIPTION

[0016] The technical solution of the present invention is further described in detail below through embodiments. These embodiments are provided for the purpose of illustrating the present invention and are not intended to limit the present invention. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative work are within the scope of protection of this application.

[0017] The experimental methods described in the examples are conventional methods unless otherwise specified; the reagents and materials described are commercially available unless otherwise specified.

[0018] Example 1 A method for preparing a bio-injury dressing based on micro-nanotechnology comprises the following steps: (1) Weigh the following raw materials in parts by weight: 18 parts by weight of bioglass, 20 parts by weight of vaseline, 12 parts by weight of liquid paraffin, 8 parts by weight of hyaluronic acid, 5 parts by weight of epidermal growth factor, 10 parts by weight of fibronectin, and 6 parts by weight of acetic acid as a pH adjuster, and set aside; (2) Add 80 parts by weight of deionized water and the pH regulator acetic acid weighed above to the fibronectin to adjust the pH value to 3.5, then add the weighed bioglass, hyaluronic acid, and epidermal growth factor and stir thoroughly to obtain a mixture 1 for later use; (3) Heat the vaseline and liquid paraffin weighed above to 60°C to obtain mixture 2, which is set aside; (4) Add the mixture 2 obtained in step (3) to the mixture 1 obtained in step (2), and stir the mixture by a stirring device to mix uniformly. The stirring speed of the stirring device is 1000 r / min, and the stirring time is 30 min to obtain a creamy dressing; (5) The nano-mesh acetate fiber is impregnated into the creamy dressing obtained in step (4) for 12 hours, and then sterilized by ultraviolet irradiation and packaged in a hose or needle tube to obtain a bio-invasive dressing based on micro-nano technology.

[0019] Example 2 A method for preparing a bio-injury dressing based on micro-nanotechnology comprises the following steps: (1) Weigh the following raw materials in parts by weight: 20 parts by weight of bioglass, 25 parts by weight of vaseline, 15 parts by weight of liquid paraffin, 10 parts by weight of hyaluronic acid, 7 parts by weight of epidermal growth factor, 15 parts by weight of fibronectin, and 10 parts by weight of phosphoric acid as a pH adjuster, and set aside; (2) Add 100 parts by weight of deionized water and the pH regulator phosphoric acid weighed above to the fibronectin to adjust the pH value to 4.0, and then add the weighed bioglass, hyaluronic acid, and epidermal growth factor and stir thoroughly to obtain a mixture 1 for later use; (3) Heat the vaseline and liquid paraffin weighed above to 70°C to obtain mixture 2, which is set aside; (4) Add the mixture 2 obtained in step (3) to the mixture 1 obtained in step (2), and stir the mixture by a stirring device to mix uniformly. The stirring speed of the stirring device is 1100 r / min, and the stirring time is 25 min to obtain a creamy dressing; (5) The nano-mesh acetate fiber is impregnated into the creamy dressing obtained in step (4) for 18 hours, and then sterilized by ultraviolet irradiation and packaged in a hose or needle tube to obtain a bio-invasive dressing based on micro-nano technology.

[0020] Example 3 A method for preparing a bio-injury dressing based on micro-nanotechnology comprises the following steps: (1) Weigh the following raw materials in parts by weight: 22 parts by weight of bioglass, 30 parts by weight of vaseline, 18 parts by weight of liquid paraffin, 12 parts by weight of hyaluronic acid, 8 parts by weight of epidermal growth factor, 18 parts by weight of fibronectin, and 12 parts by weight of triethanolamine (boric acid, acetic acid, phosphoric acid, triethanolamine, sodium hydroxide, and potassium hydroxide), and set aside; (2) Add 120 parts by weight of deionized water and the pH regulator triethanolamine weighed above to the fibronectin to adjust the pH value to 4.5, then add the weighed bioglass, hyaluronic acid, and epidermal growth factor and stir thoroughly to obtain a mixture 1 for later use; (3) Heat the vaseline and liquid paraffin weighed above to 80°C to obtain mixture 2, which is set aside; (4) Add the mixture 2 obtained in step (3) to the mixture 1 obtained in step (2), and stir the mixture by a stirring device to mix uniformly. The stirring speed of the stirring device is 1200 r / min, and the stirring time is 20 min to obtain a creamy dressing; (5) The nano-mesh acetate fiber is impregnated into the creamy dressing obtained in step (4), and after impregnation for 24 hours, the nano-mesh acetate fiber is sterilized by ultraviolet irradiation and packaged in a hose or needle tube to obtain a bio-invasive dressing based on micro-nano technology.

[0021] Comparative Example 1 The preparation method of a bioremediation dressing based on micro-nanotechnology is exactly the same as that in Example 2. The only difference compared with Example 2 is that the bioglass in step (1) is replaced by an equal amount of sodium alginate.

[0022] Comparative Example 2 The preparation method of a bio-injury dressing based on micro-nanotechnology is exactly the same as that in Example 2. The only difference compared with Example 2 is that the epidermal growth factor in step (1) is replaced by an equal amount of silica gel.

[0023] Test example The wound dressings prepared in Examples 1-3 and Comparative Examples 1-2 were used as samples and subjected to corresponding tests. The test methods are as follows, and the specific test results are shown in Table 1: (1) Tensile strength test: A tensile testing machine is used to measure the tensile strength of the sample to evaluate its strength. The tensile strength is the ratio of the force that the film withstands at the moment of stretching and breaking to the area of ​​the film. This parameter is the standard for evaluating the maximum tensile force that the bio-injury dressing can withstand; Tensile strength = F / S Where, F: tensile force; S: cross-sectional area of ​​the specimen.

[0024] (2) Antibacterial property: According to the provisions of 2.1.8 Antibacterial (Inhibitory) Test in the 2002 edition of the Technical Specifications for Disinfection, the antibacterial performance of the samples was tested. The sample dressing was continuously dissolved and diffused through agar to form different concentration gradients to show its antibacterial effect. The antibacterial ability was judged by the size of the antibacterial ring. Table 1 Performance test results of the wound dressings prepared in Examples 1-3 of the present invention and Comparative Examples 1-2 As can be seen from the data in Table 1, the mechanical properties and antibacterial properties of the bio-wound dressings prepared in Examples 1-3 of the present invention are better than those in Comparative Examples 1-2. This shows that the present invention uses bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factor, fibronectin and pH regulator as main raw materials, and prepares the above raw materials into a water-in-oil cream mixture in a certain proportion. Then, the nano-mesh acetate fiber is impregnated into the cream mixture, and after ultraviolet sterilization and encapsulation, a creamy bio-wound dressing based on micro-nano technology is obtained, which has good mechanical properties and antibacterial properties.

[0025] Finally, it should be noted that although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and that all equivalent changes and improvements made within the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A method for preparing a bio-injury dressing based on micro-nanotechnology, characterized in that: The steps include: (1) Weigh the preset weight amounts of bioglass, vaseline, liquid paraffin, hyaluronic acid, epidermal growth factor, fibronectin and pH regulator and set aside; (2) adding deionized water and a pH regulator to the fibronectin to adjust the pH value to a preset range, and then adding bioglass, hyaluronic acid, and epidermal growth factor and stirring thoroughly to obtain a mixture 1 for later use; (3) Heat vaseline and liquid paraffin to 60-80°C to obtain mixture 2, which is set aside; (4) adding the mixture 2 obtained in step (3) to the mixture 1 obtained in step (2), stirring the mixture by a stirring device to mix the mixture uniformly, and obtaining a creamy dressing; (5) The nano-mesh acetate fiber is impregnated into the creamy dressing obtained in step (4), and after impregnation for a certain period of time, the nano-mesh acetate fiber is sterilized and packaged to obtain a bio-injury dressing based on micro-nano technology.

2. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: In the step (1), the components include 18-22 parts by weight of bioglass, 20-30 parts by weight of vaseline, 12-18 parts by weight of liquid paraffin, 8-12 parts by weight of hyaluronic acid, 5-8 parts by weight of epidermal growth factor, 10-18 parts by weight of fibronectin, and 6-12 parts by weight of a pH regulator.

3. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: The pH regulator in step (1) is at least one of boric acid, acetic acid, phosphoric acid, triethanolamine, sodium hydroxide and potassium hydroxide.

4. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: The preset range of pH value in step (2) is 3.5-4.

5.

5. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: The weight portion of deionized water added in step (2) is 80-120 weight portions.

6. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: The stirring speed of the stirring device in step (4) is 1000-1200 r / min, and the stirring time is 20-30 min.

7. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: The immersion time in step (5) is 12-24 hours.

8. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: In step (5), ultraviolet irradiation is used for sterilization.

9. The method for preparing a bio-injury dressing based on micro-nanotechnology according to claim 1, characterized in that: In step (5), the packaging is performed using a hose or a needle.

10. A bio-invasive dressing based on micro-nanotechnology, characterized in that: The invention is prepared by the preparation method according to any one of claims 1 to 9.

Images