Full-automatic solid-phase extraction instrument

Abstract

The full-automatic solid-phase extraction instrument comprises a box body and a plurality of extraction units, an extraction cavity and a mounting cavity located below the extraction cavity are formed in the box body, an extraction column mounting frame is arranged in the extraction cavity, and a plurality of extraction columns are mounted on the extraction column mounting frame; the extraction unit comprises a three-way pipe, a collecting box, a first lifting device, a second lifting device, a third lifting device and a left-right driving device, the collecting box is placed in the extraction cavity, a waste liquid pipe containing barrel and a storage pipe containing barrel are arranged in the collecting box, the three-way pipe comprises a vertical pipe and a transverse pipe which are connected, a first valve is installed at the bottom end of the vertical pipe, and a second valve is installed at the left end of the transverse pipe; a through hole is formed in the side wall of the storage pipe, a horizontal branch pipe is connected to the through hole, a sealing head is arranged at the top end of the storage pipe, and the lifting device I is mounted on the extraction column mounting frame. According to the device, after activation is completed, a target analyte can be directly discharged into the storage tube for sealing, so that the volatilization of volatile substances is reduced, and the recovery rate of the volatile substances is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of extraction devices, in particular to a full-automatic solid phase extraction instrument. Background Art

[0002] The solid phase extractor is an instrument used in fields such as environmental and food science. It effectively integrates all steps of solid phase extraction into a single platform, enabling fully automated operation of the entire solid phase extraction process. This significantly improves the efficiency of sample pretreatment, freeing analysts from frequent pretreatment tasks and making sample pretreatment faster and more efficient.

[0003] Solid-phase extraction (SPE) cartridges are the core consumables and tools of SPE technology. They are small chromatographic columns whose core function is to utilize a special adsorbent (stationary phase) packed within the column to selectively adsorb target compounds from the sample solution through physical or chemical reactions, while simultaneously removing matrix interferences or impurities to achieve separation of the target analyte.

[0004] The solid phase extraction process includes activation, sample loading, washing and elution.

[0005] Activation transforms the surface of the hydrophobic filler from a dry state to a solvated state, exposing its binding sites. Unactivated hydrophobic fillers may repel the sample solution in water, preventing the target analyte from effectively adsorbing or even penetrating.

[0006] Loading is the process of passing the sample solution to be processed through the activated solid phase extraction column in a controlled manner, so that the target analyte is selectively adsorbed on the column bed by the filler.

[0007] Elution involves flushing the SPE column with a selective solvent to elute and remove matrix-interfering impurities that are weakly adsorbed to the packing material and then nonspecifically adsorbed. While the target analytes should be firmly retained on the column, elution is an important step in improving purity.

[0008] The purpose of elution is to selectively dissolve and wash the target analytes adsorbed on the solid phase extraction column filler with an appropriate solvent to complete the recovery of the target analytes.

[0009] After the activation, loading, and elution processes are complete, waste liquid needs to be collected. After the elution process is complete, the target analyte needs to be recovered. Current fully automatic solid-phase extraction systems must first discharge the target analyte into a recovery container, which is then transferred to a storage tube for sealed storage. During this process, volatile substances are exposed to the air and evaporate in large quantities, resulting in a sharp drop in the recovery rate of the target analyte after collection. Summary of the Invention

[0010] In response to the defects in the prior art, the present invention provides a fully automatic solid phase extraction device. After activation is completed, the device can directly discharge the target analyte into a storage tube for sealing, thereby reducing the volatilization of volatile substances and improving the recovery rate of volatile substances.

[0011] A fully automatic solid-phase extractor comprises a housing and a plurality of extraction units. The housing comprises an extraction chamber and a mounting chamber below the extraction chamber. The front side of the extraction chamber is open. An extraction column mounting rack is provided in the extraction chamber. A plurality of extraction columns are mounted on the extraction column mounting rack. The extraction units correspond to the extraction columns one by one.

[0012] The extraction unit includes a three-way pipe, a collection box, a lifting device 1, a lifting device 2, a lifting device 3 and left and right driving devices.

[0013] The collecting box is placed in the extraction chamber, and the collecting box has a waste liquid pipe accommodating cylinder and a storage pipe accommodating cylinder. The waste liquid pipe accommodating cylinder is placed in the waste liquid pipe accommodating cylinder, and the storage pipe is placed in the storage pipe accommodating cylinder. The lifting device 2 and the lifting device 3 are located in the installation chamber.

[0014] The bottom wall of the collection box has a through hole 1, and the bottom wall of the extraction chamber has a through hole 2. The through hole 1 corresponds to the through hole 2, and the output shaft of the lifting device 2 can pass through the through hole 1 and the through hole 2 to contact the waste liquid pipe.

[0015] The bottom wall of the collection box has a through hole three, and the bottom wall of the extraction chamber has a through hole four. Through hole three corresponds to through hole four, and the output shaft of the lifting device three can pass through through hole three and through hole four to contact the storage tube.

[0016] The three-way pipe includes a vertical pipe and a horizontal pipe connected to each other. The top of the vertical pipe is connected to the connecting pipe, and the top of the connecting pipe is connected to the bottom of the extraction column. Valve 1 is installed at the bottom of the vertical pipe, and valve 2 is installed at the left end of the horizontal pipe. An annular cylinder is provided on the outer sleeve of the horizontal pipe. The left and right driving devices can drive the annular cylinder to move left and right.

[0017] The side wall of the storage tube is provided with a through hole, the through hole is connected to the horizontal branch pipe, the top of the storage tube is provided with a sealing head, the lifting device 1 is installed on the extraction column mounting frame, and the lifting device 1 is located above the sealing head.

[0018] Preferably, the extraction column mounting frame is connected to a mounting plate, left and right driving devices are fixed on the mounting plate, and output shafts of the left and right driving devices are connected to driving rods, which are connected to the annular cylinder.

[0019] Preferably, a mounting groove is provided on the bottom wall of the extraction chamber, and the collection box is placed in the mounting groove.

[0020] Preferably, the top wall of the sealing head has a limiting groove, and the bottom end of the output shaft of the lifting device 1 enters the limiting groove.

[0021] Preferably, the output shafts of the second lifting device and the third lifting device are both connected with support blocks.

[0022] Preferably, the sealing head is made of rubber material.

[0023] Preferably, the lifting device 1, the lifting device 2, the lifting device 3 and the left and right driving devices are all cylinders.

[0024] The beneficial effects of the present invention are as follows: in this technical solution, through the cooperation between the extraction unit and the extraction column, when in use, the collection box is placed in the extraction chamber, the lifting device 2 and the lifting device 3 are started, and the output shaft of the lifting device 2 presses the waste liquid pipe to move upward until the bottom end of the vertical pipe enters the waste liquid pipe, and the output shaft of the lifting device 3 presses the storage pipe to move upward until the horizontal branch pipe and the horizontal pipe are at the same horizontal height; the left and right driving devices are started, and the left and right driving devices drive the annular cylinder to move, so that the annular cylinder is simultaneously mounted on the horizontal pipe and the horizontal branch pipe; when collecting waste liquid, valve one is opened and valve two is closed, and the waste liquid enters the waste liquid pipe for collection; when collecting target analytes, valve one is closed and valve two is opened, and the target analytes enter the storage tube; after the target analytes are collected, the lifting device 1 is started, and the output shaft of the lifting device 1 presses the sealing head to move downward, and the sealing head seals the through hole on the side wall of the storage tube to achieve storage of the target analytes. In this way, after activation is completed, the device can directly discharge the target analyte into the storage tube for sealing, thereby reducing the volatilization of volatile substances and improving the recovery rate of volatile substances. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the specific embodiments or the description of the prior art. Similar elements or parts are generally identified by similar reference numerals throughout the drawings. Elements or parts in the drawings are not necessarily drawn to scale.

[0026] Figure 1 It is the front view of the present invention;

[0027] Figure 2 for Figure 1 Schematic diagram of the enlarged structure of position A in the middle.

[0028] In the accompanying drawings, 1-box, 2-extraction chamber, 3-mounting chamber, 4-extraction column mounting bracket, 5-extraction column, 6-connecting pipe, 7-tee pipe, 8-collecting box, 9-waste liquid pipe accommodating cylinder, 10-storage tube accommodating cylinder, 11-waste liquid pipe, 12-storage pipe, 13-horizontal branch pipe, 14-sealing head, 15-lifting device 1, 16-lifting device 2, 17-lifting device 3, 18-annular cylinder, 19-left and right driving devices, 20-mounting plate, 21-valve 1, 22-valve 2. DETAILED DESCRIPTION

[0029] The following embodiments of the technical solution of the present invention will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention and are therefore only examples and are not intended to limit the scope of protection of the present invention.

[0030] It should be noted that, unless otherwise specified, the technical or scientific terms used in this application should have the common meanings understood by those skilled in the art to which the present invention belongs.

[0031] Example 1

[0032] like Figure 1-Figure 2 As shown, this embodiment provides a fully automatic solid phase extractor, comprising a housing 1 and a plurality of extraction units. The housing 1 has an extraction chamber 2 and a mounting chamber 3 located below the extraction chamber 2. The front side of the extraction chamber 2 is open. An extraction column mounting rack 4 is provided in the extraction chamber 2. A plurality of extraction columns 5 are mounted on the extraction column mounting rack 4. The extraction units correspond to the extraction columns 5 one by one.

[0033] The extraction unit includes a three-way pipe 7, a collecting box 8, a lifting device 15, a lifting device 2 16, a lifting device 3 17 and left and right driving devices 19.

[0034] The collecting box 8 is placed in the extraction chamber 2. The collecting box 8 has a waste liquid pipe accommodating cylinder 9 and a storage pipe accommodating cylinder 10. The waste liquid pipe accommodating cylinder 9 is provided with a waste liquid pipe 11. The storage pipe accommodating cylinder 10 is provided with a storage pipe 12. The second lifting device 16 and the third lifting device 17 are located in the installation chamber 3.

[0035] The bottom wall of the collecting box 8 has a through hole 1, and the bottom wall of the extraction chamber 2 has a through hole 2. The through hole 1 corresponds to the through hole 2, and the output shaft of the lifting device 2 16 can pass through the through hole 1 and the through hole 2 to contact the waste liquid pipe 11.

[0036] The bottom wall of the collecting box 8 has a through hole 3, and the bottom wall of the extraction chamber 2 has a through hole 4. The through hole 3 corresponds to the through hole 4. The output shaft of the lifting device 3 17 can pass through the through hole 3 and the through hole 4 to contact the storage tube 12.

[0037] The three-way pipe 7 includes a vertical pipe and a horizontal pipe connected to each other. The top of the vertical pipe is connected to the connecting pipe 6, and the top of the connecting pipe 6 is connected to the bottom of the extraction column 5. The bottom of the vertical pipe is installed with a valve 1 21, and the left end of the horizontal pipe is installed with a valve 2 22. The horizontal pipe is outer-mounted with an annular cylinder 18. The left and right driving devices 19 can drive the annular cylinder 18 to move left and right.

[0038] The side wall of the storage tube 12 is provided with a through hole, the through hole is connected to the horizontal branch pipe 13, the top of the storage tube 12 is provided with a sealing head 14, and a lifting device 15 is installed on the extraction column mounting frame 4, and the lifting device 15 is located above the sealing head 14.

[0039] In this embodiment, the extraction unit cooperates with the extraction column 5, and the specific working principle is as follows:

[0040] The top of the vertical pipe of the tee pipe 7 is connected to the bottom of the extraction column 5 through the connecting pipe 6, and the bottom of the sealing head 14 is located above the horizontal branch pipe 13.

[0041] Place the collection box 8 in the extraction chamber 2, and activate the second and third lifting devices 16 and 17. The output shaft of the second lifting device 16 passes through the first and second through-holes to contact the waste liquid pipe 11, and pushes the waste liquid pipe 11 upward until the bottom end of the vertical pipe enters the waste liquid pipe 11. The output shaft of the third lifting device 17 passes through the third and fourth through-holes to contact the storage pipe 12, and pushes the storage pipe 12 upward until the horizontal branch pipe 13 and the horizontal pipe are at the same level.

[0042] Start the left and right drive devices 19, which drive the annular cylinder 18 to move, so that the annular cylinder 18 is simultaneously sleeved on the horizontal pipe and the horizontal branch pipe 13;

[0043] During the activation, sampling and elution processes, valve 1 21 is opened and valve 2 22 is closed. The waste liquid enters the waste liquid pipe 11 through the connecting pipe 6 and the vertical pipe for collection. During the elution process, valve 1 21 is closed and valve 2 22 is opened. At this time, the target analyte enters the storage tube 12 through the connecting pipe 6, the upper section of the vertical pipe, the horizontal pipe and the horizontal branch pipe 13. After the target analyte is collected, the lifting device 15 is started, and the output shaft of the lifting device 15 presses against the sealing head 14 to move downward. The sealing head 14 seals the through hole in the side wall of the storage tube 12, thereby achieving storage of the target analyte.

[0044] In this way, the device can realize the integrated collection of waste liquid and storage of target analytes. After activation is completed, the target analytes are directly discharged into the storage tube 12 for sealing, thereby reducing the volatilization of volatile substances and improving the recovery rate of volatile substances.

[0045] In this embodiment, both valve 1 21 and valve 2 22 are solenoid valves to achieve overall chip control.

[0046] In this embodiment, a filter element is provided in the transverse tube. After elution, the target analyte is filtered and discharged into the storage tube 12, thereby improving the purity of the target analyte.

[0047] In this embodiment, the extraction column mounting frame 4 is connected to a mounting plate 20, to which a left and right drive mechanism 19 is fixed. Drive rods are connected to the output shafts of the left and right drive mechanisms 19, and the drive rods are connected to the annular cylinder 18. In this embodiment, the mounting plate 20 is provided for mounting the left and right drive mechanisms 19, which are located to the left of the extraction column 8, thereby effectively utilizing the space.

[0048] In this embodiment, the bottom wall of the extraction chamber 2 is provided with a mounting groove, into which the collection box 8 is placed. The mounting groove is provided in this embodiment to position the collection box 8. When the collection box 8 is placed in the mounting groove, the second lifting device 16 corresponds to the waste liquid pipe 11, and the third lifting device 17 corresponds to the storage pipe 12. The waste liquid pipe 11 is located below the vertical pipe, and the storage pipe 12 is located below the first lifting device 15.

[0049] In this embodiment, the top wall of the sealing head 14 has a retaining groove, into which the bottom end of the output shaft of the lifting device 15 enters. In this embodiment, the retaining groove is provided so that when the horizontal branch pipe 13 and the transverse pipe of the tee pipe 7 are at the same level, the output shaft of the lifting device 15 enters the retaining groove. At this time, the storage pipe 12 is restrained vertically by the lifting device 15 and the lifting device 3 17. Simultaneously, the output shaft of the lifting device 15 enters the retaining groove to restrain the storage pipe 12 left and right, thereby improving the stability of the storage pipe 12 and allowing the annular cylinder 18 to be stably mounted on the horizontal branch pipe 13.

[0050] In this embodiment, support blocks are connected to the output shafts of the lifting device 2 16 and the lifting device 3 17. In this embodiment, the support blocks are connected to the output shafts of the lifting device 2 16 and the lifting device 3 17. During use, the support blocks support the waste liquid pipe 11 and the storage pipe 12, and the support is more stable.

[0051] In this embodiment, the sealing head 14 is made of a rubber material. In this embodiment, the sealing head 14 is made of a rubber material. The rubber material and the storage tube 12 have an interference fit to ensure the sealing performance of the storage tube 12.

[0052] In this embodiment, the lifting device 15, the lifting device 2 16, the lifting device 3 17 and the left and right drive devices 19 are all cylinders. In this embodiment, the lifting device and the left and right drive devices are all cylinders, and multi-stage cylinders are used to reduce space occupation and facilitate control.

[0053] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention, and they should all be included in the scope of the claims and description of the present invention.

Claims

1. A fully automatic solid phase extraction instrument, characterized in that, The invention comprises a housing (1) and a plurality of extraction units, wherein the housing (1) has an extraction chamber (2) and a mounting chamber (3) located below the extraction chamber (2), the front side of the extraction chamber (2) is open, an extraction column mounting frame (4) is provided in the extraction chamber (2), a plurality of extraction columns (5) are mounted on the extraction column mounting frame (4), and the extraction units correspond to the extraction columns (5) on a one-to-one basis; The extraction unit comprises a three-way pipe (7), a collecting box (8), a lifting device 1 (15), a lifting device 2 (16), a lifting device 3 (17) and left and right driving devices (19). The collecting box (8) is placed in the extraction chamber (2), and the collecting box (8) has a waste liquid pipe accommodating cylinder (9) and a storage pipe accommodating cylinder (10). The waste liquid pipe accommodating cylinder (9) is placed in the waste liquid pipe accommodating cylinder (11), and the storage pipe accommodating cylinder (10) is placed in the storage pipe accommodating cylinder (12). The second lifting device (16) and the third lifting device (17) are located in the installation chamber (3). The bottom wall of the collecting box (8) has a through hole 1, and the bottom wall of the extraction chamber (2) has a through hole 2, wherein the through hole 1 corresponds to the through hole 2, and the output shaft of the lifting device 2 (16) can pass through the through hole 1 and the through hole 2 to contact the waste liquid pipe (11). The bottom wall of the collecting box (8) has a through hole three, and the bottom wall of the extraction chamber (2) has a through hole four, and the through hole three corresponds to the through hole four. The output shaft of the lifting device (17) can pass through the through hole three and the through hole four to contact the storage tube (12). The three-way pipe (7) includes a vertical pipe and a horizontal pipe connected to each other. The top of the vertical pipe is connected to the connecting pipe (6), and the top of the connecting pipe (6) is connected to the bottom of the extraction column (5). The bottom of the vertical pipe is installed with a valve 1 (21), and the left end of the horizontal pipe is installed with a valve 2 (22). The horizontal pipe is provided with an annular cylinder (18) on its outer sleeve. The left and right driving devices (19) can drive the annular cylinder (18) to move left and right. The side wall of the storage tube (12) is provided with a through hole, the through hole is connected to the horizontal branch pipe (13), the top of the storage tube (12) is provided with a sealing head (14), and a lifting device (15) is installed on the extraction column mounting frame (4), and the lifting device (15) is located above the sealing head (14).

2. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The extraction column mounting frame (4) is connected to a mounting plate (20), and left and right drive devices (19) are fixed on the mounting plate (20). The output shafts of the left and right drive devices (19) are connected to drive rods, and the drive rods are connected to the annular cylinder (18).

3. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The bottom wall of the extraction chamber (2) is provided with a mounting groove, and the collection box (8) is placed in the mounting groove.

4. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The top wall of the sealing head (14) is provided with a limiting groove, and the bottom end of the output shaft of the lifting device (15) enters the limiting groove.

5. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The output shafts of the lifting device 2 (16) and the lifting device 3 (17) are both connected with support blocks.

6. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The sealing head (14) is made of rubber material.

7. A fully automatic solid phase extraction instrument according to claim 1, characterized in that, The lifting device 1 (15), the lifting device 2 (16), the lifting device 3 (17) and the left and right driving devices (19) are all cylinders.

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