Debugging device and lens debugging method

Abstract

The invention discloses a debugging device and a lens debugging method, the debugging device is used for debugging a lens installed in a lens seat, and the debugging device comprises a base which comprises a positioning part and a lens seat installation part, and the lens seat installation part is used for installing the lens seat; the marking assembly is movably mounted on the positioning part, the marking assembly comprises a marking part, the marking part comprises one or more concentric circle graduated scales which are arranged in an arrayed mode, each concentric circle graduated scale comprises a plurality of concentric circles with different diameters, and the lens base mounting part is located on the upstream side of the marking assembly in the incident direction of light rays to the marking assembly; the posture of the lens is debugged according to the position relation between a light spot formed by light rays irradiating the marking part through the lens and concentric circles in the concentric circle graduated scale. The posture of the lens is adjusted according to the position relation between the light spot formed by the light irradiated to the marking part and the concentric circle in the concentric circle graduated scale, so that the lens of the lens seat can be adjusted to be qualified.

Description

Technical Field

[0001] The present application relates to the field of debugging devices, and in particular to a debugging device and a lens debugging method. Background Art

[0002] Flow cytometers use a series of optical components to analyze the fluorescence signals of cells or particles through spectral detection technology. This combination of capturing the complete emission spectrum of fluorescent dyes and analyzing overlapping spectra with algorithms can significantly improve multicolor detection capabilities and data accuracy. These optical components include the lens holder, through which light passes to illuminate the target location during detection.

[0003] Accurate installation of the lens is conducive to improving the accuracy of the detection results of the flow cytometer. If the assembled lens can be debugged during the production stage, the yield rate of the flow cytometer can be improved. Summary of the Invention

[0004] In order to solve the above technical problems, the present application provides a debugging device and a lens debugging method. The debugging device of the present application can test and adjust the lens of the lens holder.

[0005] This application is implemented through the following technical solutions.

[0006] The first aspect of the present application provides a debugging device for debugging a lens installed in a lens seat, the debugging device comprising: a base comprising a positioning portion and a lens seat mounting portion, the lens seat mounting portion being used to mount the lens seat; a marking assembly movably mounted on the positioning portion, the marking assembly comprising a marking portion, the marking portion comprising one or more arranged concentric circle scales, each of the concentric circle scales comprising a plurality of concentric circles with different diameters, wherein, along the incident direction of light to the marking assembly, the lens seat mounting portion is located on the upstream side of the marking assembly, and the posture of the lens is debugged according to the positional relationship between a light spot formed by light passing through the lens and irradiating the marking portion and the concentric circles in the concentric circle scales.

[0007] By adjusting the posture of the lens according to the positional relationship between the light spot formed by the light irradiating the marking portion through the lens and the concentric circles in the concentric circle scale, the center of the light spot and the center of the concentric circle overlap, thereby adjusting the lens of the lens holder to be qualified and improving the yield rate of the lens holder.

[0008] The marking component is movable in the positioning portion, and the size of the light spot can be adjusted by moving. When the light spot is adjusted to be larger, it is helpful to determine whether the center of the light spot overlaps with the center of the concentric circle. When the light spot is adjusted to be smaller, it is helpful to determine the concentric circle to be compared with the light spot.

[0009] In some embodiments of the present application, the positioning portion has a guide mechanism, and the marking assembly is disposed on the guide mechanism and is movable along the guide mechanism.

[0010] The guide mechanism can provide guidance for the setting position of the marking component, which is conducive to the accurate setting of the marking component.

[0011] In some embodiments of the present application, the marking assembly includes a supporting portion, the supporting portion is arranged on the positioning portion, the marking portion is erected on the supporting portion, and the supporting portion includes a first limiting portion; the positioning portion includes a second limiting portion, and the second limiting portion is adapted to the first limiting portion to limit the position of the supporting portion relative to the lens seat along the incident direction of light to the marking assembly.

[0012] Limiting the marking component to move in a direction close to or away from the lens seat is beneficial for the marking component to move along the incident direction of the light to the marking component to adjust the size of the light spot.

[0013] In some embodiments of the present application, the first limiting portion is a limiting groove; the second limiting portion includes a plurality of limiting protrusions, and the plurality of limiting protrusions are arranged at intervals along the incident direction of light toward the marking component, and the limiting groove accommodates some of the plurality of limiting protrusions.

[0014] The plurality of limiting protrusions are arranged at intervals, and the moving distance of the marking assembly can be adjusted by the distance between the plurality of limiting protrusions.

[0015] In some embodiments of the present application, the marking assembly includes a first marking assembly and a second marking assembly, and the first marking assembly and the second marking assembly each include multiple marking parts; the positioning part includes a first positioning part and a second positioning part, the first marking assembly is detachably provided on the first positioning part, and the second marking assembly is detachably provided on the second positioning part, and the lens seat mounting part includes a first lens seat mounting part for mounting the first lens seat, and the first lens seat mounting part is located between the first positioning part and the second positioning part, and the light emitted from the lenses on both sides of the first lens seat is respectively irradiated to the marking part of the first marking assembly and the marking part of the second marking assembly.

[0016] The provision of the first positioning portion and the second positioning portion can facilitate adjustment of the lens of the lens holder capable of emitting light from both sides.

[0017] In some embodiments of the present application, the base includes a first light source mounting portion, which is used to mount a light source assembly; the marking assembly also includes a third marking assembly, which is detachably arranged on the first positioning portion. Along the incident direction of the light to the third marking assembly, the first light source mounting portion is located on the upstream side of the third marking assembly. The positional relationship between the light spot formed by the light emitted from the light source assembly to the marking portion of the third marking assembly and the concentric circles in the concentric circle scale of the first marking assembly is used to adjust the posture of the light source assembly.

[0018] Providing the third marking component is beneficial to adjusting the posture of the light source component to improve the yield rate of the light source component.

[0019] The third marking component can be arranged on the first positioning portion, which fully utilizes the arrangement of the first positioning portion, can save space on the base, and is conducive to miniaturization of the base.

[0020] In some embodiments of the present application, the debugging device includes a spectroscopic component, which is arranged opposite to the first light source mounting portion. Along the incident direction of the light to the first lens seat mounting portion, the spectroscopic component is located on the upstream side of the first lens seat mounting portion. The light emitted by the light source component passes through the spectroscopic component and is emitted as a first light and a second light. The first light is emitted after passing through the lens of the first lens seat and is respectively irradiated to the marking portion of the first marking component and the marking portion of the second marking component. The posture of the lens of the first lens seat is tested, adjusted and debugged according to the positional relationship between the light spot formed by the first light irradiated to the marking portion of the first marking component and the marking portion of the second marking component and the concentric circles in the concentric circle scale; the marking component includes a fourth marking component and a fifth marking component, and the fourth marking component and the fifth marking component each include a plurality of marking parts; the fixed The positioning portion includes a third positioning portion and a fourth positioning portion, the fourth marking assembly is detachably arranged on the third positioning portion, and the fifth marking assembly is detachably arranged on the fourth positioning portion, the lens seat mounting portion includes a second lens seat mounting portion for mounting a second lens seat, the second lens seat is arranged between the third positioning portion and the fourth positioning portion, along the incident light path of the light to the second lens seat mounting portion, the spectroscopic assembly is located on the upstream side of the second lens seat mounting portion, the second light emitted from the spectroscopic assembly passes through the lenses on both sides of the second lens seat and then emits light that is respectively irradiated to the marking portion of the fourth marking assembly and the marking portion of the fifth marking assembly, and the posture of the lens of the second lens seat is tested, adjusted and debugged according to the positional relationship between the light spot formed by the second light irradiated to the marking portion of the fourth marking assembly and the marking portion of the fifth marking assembly and the concentric circles in the concentric circle scale.

[0021] The light emitted by the light source assembly is divided into a first light and a second light by using a light splitting assembly, and the installation of the lenses of the first lens seat and the second lens seat can be detected and adjusted at the same time, thereby improving the efficiency of debugging the lenses.

[0022] In addition, in some flow cytometers, the light from the light source assembly is divided into two beams of light through a spectroscopic element to pass through two lens holders for testing. Such a setting in the present application is conducive to simulating real situations, thereby improving the accuracy of the lenses of the lens holder in actual use.

[0023] In some embodiments of the present application, the debugging device includes a first refractive component and a second refractive component; along the incident direction of the first light ray to the first lens seat mounting portion, the first refractive component is located on the upstream side of the first lens seat mounting portion and between the spectroscopic component and the first lens seat mounting portion, the first light ray emitted from the spectroscopic component is incident on the first refractive component and is refracted by the first refractive component before being incident on the first lens seat; along the incident direction of the second light ray to the second lens seat mounting portion, the second refractive component is located on the upstream side of the second lens seat mounting portion and between the spectroscopic component and the second lens seat mounting portion, the second light ray emitted from the spectroscopic component is incident on the second refractive component and is refracted by the second refractive component before being incident on the second lens seat.

[0024] In some embodiments of the present application, the base includes a light source testing portion, the light source testing portion includes a fifth positioning portion and a second light source mounting portion, the light source testing portion is located at the edge of the base, the second light source mounting portion is used to install the light source assembly, and the second light source mounting portion is arranged opposite to the fifth positioning portion; the third marking assembly is detachably arranged on the fifth positioning portion, and the posture of the light source assembly is adjusted according to the positional relationship between the light spot formed by the light emitted from the light source assembly installed on the second light source mounting portion and the light on the marking portion of the third marking assembly and the concentric circles in the concentric circle scale of the third marking assembly.

[0025] The light source testing part is located at the edge of the base. On the one hand, the light source assembly can be debugged. On the other hand, the light source assembly can be placed on the first light source mounting part after being debugged in the light source testing part. Compared with debugging the light source assembly at the first light source mounting part and then continuing to debug the lens seat, this method can improve efficiency.

[0026] In some embodiments of the present application, the debugging device includes a fixing component, which is used to fix the lens of the lens seat, and the fixing component includes: a pressing member, which is used to be detachably connected to the lens seat to fix the lens of the lens seat, and the pressing member includes a plurality of light-through holes, and the plurality of light-through holes correspond one-to-one to the plurality of lenses; a flexible member, which is used to fit the lens, and the flexible member is clamped between the pressing member and the lens.

[0027] The flexible member is sandwiched between the pressing member and the plurality of lenses, which can reduce the pressure of the pressing member on the lenses and avoid damage to the lenses due to excessive pressure.

[0028] In addition, the lens can be observed and adjusted through the light hole to ensure accurate bonding of the lens.

[0029] In some embodiments of the present application, the fixing assembly includes a fastener, which is disposed on the pressing member to fix the pressing member to the lens holder.

[0030] In some embodiments of the present application, the pressing member includes a first part and a second part connected to each other, the first part and the second part extend in different directions respectively, the flexible member is clamped between the first part and the lens, and the fastener is arranged in the second part and fixed to the lens seat.

[0031] The first portion and the second portion extend in different directions, and the lens can be fixed in different directions.

[0032] In some embodiments of the present application, the pressing member includes: a first pressing member for fixing a plurality of first lenses located on the first side of the lens seat, the first pressing member including a plurality of first light-through holes, and the plurality of first light-through holes correspond one-to-one to the plurality of first lenses; a second pressing member for fixing a plurality of second lenses located on the second side of the lens seat, the second pressing member including a plurality of second light-through holes, and the plurality of second light-through holes correspond one-to-one to the plurality of second lenses, and the first side is opposite to the second side; the flexible member includes: a first flexible member for fitting to the plurality of first lenses, the first flexible member being clamped between the first pressing member and the plurality of first lenses; a second flexible member for fitting to the plurality of second lenses, the second flexible member being clamped between the second pressing member and the plurality of second lenses.

[0033] A second aspect of the present application provides a method for debugging a lens, the method comprising: irradiating debugging light through the lens of the lens holder to a marking component; moving the marking component along the positioning portion to observe the overlapping range of the light spot and the concentric circles, wherein the marking component comprises one or more marking portions, the marking portion comprising a plurality of concentric circle scales of different diameters arranged in an arranged manner, each of the concentric circle scales comprising a plurality of concentric circles of different diameters; determining that the lens of the lens holder is qualified based on the overlap between the center of the light spot formed by the light irradiating the marking component and the center of the concentric circle, and adjusting the lens of the lens holder based on the fact that the center of the light spot formed by the light irradiating the marking component does not overlap with the center of the concentric circle until the center of the light spot formed by the light irradiating the marking component coincides with the center of the concentric circle.

[0034] Through the method of the present application, it is possible to test whether the lenses of the lens holder are installed properly, and it is also possible to adjust unqualified lenses to qualified ones, thereby improving the yield rate of the lens holder.

[0035] In some embodiments of the present application, the marking component includes a light source marking component, and the method further includes: irradiating the light source marking component with debugging light emitted by the light source component; causing the light source marking component to move along the positioning portion to observe the overlapping range of the light spot and the concentric circle; determining that the light source component is qualified based on the overlap between the center of the light spot formed by the light irradiating the light source marking component and the center of the concentric circle of the light source marking component, and adjusting the light emitted by the light source component based on the fact that the center of the light spot formed by the light irradiating the light source marking component and the center of the concentric circle of the light source marking component do not overlap.

[0036] The method of the present application can be used to test and adjust the light source assembly so that the angle of the light emitted by the light source assembly meets the requirements of the flow cytometer.

[0037] In some embodiments of the present application, the method further includes: adhering a plurality of lenses to the lens seat; attaching a flexible member to the lenses; fixing a pressing member to the lens seat so that the flexible member is clamped between the pressing member and the lens seat until the lenses are fixed to the lens seat.

[0038] According to the method of the present application, when fixing lenses, since the flexible member is sandwiched between the pressing member and the plurality of lenses, the pressure of the pressing member on the lenses can be reduced, thereby avoiding damage to the lenses due to excessive pressure. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present application. The same reference numerals are used throughout the drawings to denote the same components. In the drawings:

[0040] Figure 1 A schematic top view of the debugging device provided in some embodiments of the present application;

[0041] Figure 2 A schematic diagram of the three-dimensional structure of the debugging device provided in some embodiments of the present application from a first perspective;

[0042] Figure 3 A schematic top view of the structure of a debugging device provided in other embodiments of the present application;

[0043] Figure 4 A schematic diagram of the third perspective structure of the debugging device provided in some embodiments of the present application from a second perspective;

[0044] Figure 5 A schematic diagram of the three-dimensional structure of a marking assembly provided in some embodiments of the present application;

[0045] Figure 6 Schematic diagrams of the three-dimensional structure of the marking assembly provided in other embodiments of the present application;

[0046] Figure 7 A schematic diagram of the three-dimensional structure of a fixing assembly and a lens holder provided in some embodiments of the present application;

[0047] Figure 8 A schematic top view of the fixing assembly and lens holder provided in some embodiments of the present application;

[0048] Figure 9 A schematic side view of the structure of a fixing assembly and a lens holder provided in some embodiments of the present application;

[0049] Figure 10 To follow Figure 9 A schematic cross-sectional view of the structure taken along line AA;

[0050] Figure 11 A block diagram of a lens debugging method provided in some embodiments of the present application;

[0051] Figure 12 A block diagram of a lens debugging method provided in some other embodiments of the present application;

[0052] Figure 13 A block diagram of a lens debugging method provided in some further embodiments of the present application.

[0053] Description of Reference Numerals

[0054] 10. Base; 100. Light source test unit; 110. Positioning unit; 11. Spectral assembly; 111. First positioning unit; 112. Second positioning unit; 113. Third positioning unit; 114. Fourth positioning unit; 115. Fifth positioning unit; 1100. Guide mechanism; 1101. First groove wall; 1102. Second groove wall; 1103. Third groove wall; 1104. Fourth groove wall; 1105. Fifth groove wall; 120. Second limiting unit; 130. Lens mounting unit; 131. First lens holder mounting unit; 132. Second lens holder mounting unit; 141. First light source mounting unit; 142. Second light source mounting unit; 151. First refraction assembly; 152. Second refraction assembly; 2. Lens holder; 20. Marking group Parts; 21. Lens; 210. First limiting portion; 211. First marking assembly; 222. Second marking assembly; 233. Third marking assembly; 244. Fourth marking assembly; 255. Fifth marking assembly; 22. Bracket of lens holder; 201. Marking portion; 202. Support portion; 203. Concentric circle scale; 204. Concentric circles; 2000. Focusing lens; 2001. First lens holder; 2002. Second lens holder; 3. Light source assembly; 41. Flexible part; 411. First flexible part; 412. Second flexible part; 42. Pressing part; 421. First pressing part; 422. Second pressing part; 43. Fastener; 44. Light hole; 401. First part; 402. Second part; L. Light path. DETAILED DESCRIPTION

[0055] The following embodiments of the technical solution of the present application will be described in detail with reference to the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application and are therefore only examples and are not intended to limit the scope of protection of the present application.

[0056] Flow cytometers use a series of optical components to analyze the fluorescence signals of cells or particles through spectral detection technology. This combination of capturing the complete emission spectrum of fluorescent dyes and analyzing overlapping spectra with algorithms can significantly improve multicolor detection capabilities and data accuracy. These optical components include the lens holder, through which light passes to illuminate the target location during detection.

[0057] Accurate installation of the lens is conducive to improving the accuracy of the detection results of the flow cytometer. If the assembled lens can be debugged during the production stage, the yield rate of the flow cytometer can be improved.

[0058] In order to solve the above technical problems, the present application provides a debugging device and a lens debugging method. The debugging device of the present application can test and adjust the lens of the lens holder.

[0059] The debugging device provided in the present application is used for debugging a lens installed in a lens seat, and the debugging device includes: a base, including a positioning portion and a lens seat mounting portion, and the lens seat mounting portion is used to install the lens seat; a marking assembly, movably mounted on the positioning portion, and the marking assembly includes a marking portion, and the marking portion includes one or more arranged concentric circle scales, each concentric circle scale includes a plurality of concentric circles with different diameters, wherein, along the incident direction of the light to the marking assembly, the lens seat mounting portion is located on the upstream side of the marking assembly, and the posture of the lens is debugged according to the positional relationship between the light spot formed by the light passing through the lens to the marking portion and the concentric circles in the concentric circle scale.

[0060] By adjusting the posture of the lens according to the positional relationship between the light spot formed by the light shining through the lens onto the marking part and the concentric circles in the concentric circle scale, the center of the light spot and the center of the concentric circle overlap, thereby adjusting the lens of the lens holder to be qualified and improving the yield rate of the lens holder.

[0061] The marking component is movable in the positioning portion, and the size of the light spot can be adjusted by moving. When the light spot is adjusted to be larger, it is helpful to determine whether the center of the light spot overlaps with the center of the concentric circle. When the light spot is adjusted to be smaller, it is helpful to determine the concentric circle to be compared with the light spot.

[0062] like Figures 1 to 4 As shown, the debugging device of the present application may include a base 10 and a marking assembly 20, and the marking assembly 20 may be detachably mounted on the base 10. The base 10 may include a positioning portion 110 and a lens holder mounting portion 130, the marking assembly 20 may be detachably mounted on the positioning portion 110, and the lens holder 2 may be mounted on the lens holder mounting portion 130.

[0063] like Figures 7 to 10 As shown, the lens holder 2 of the present application may have a first side and a second side that are opposite to each other, one or more lenses 21 may be provided on the first side, and one or more lenses 21 may be provided on the second side.

[0064] In the present application, a plurality of lenses may be provided on the first side of the lens holder 2, wherein one of the plurality of lenses may be a focusing lens (adjusting lens), and the rest may be translucent lenses. The focusing lens may be configured to be adjustable and reflect light. The reflection of the focusing lens may adjust the angle at which light incident on the lens holder 2 is emitted from the translucent lens.

[0065] In the present application, a plurality of lenses may be provided on the second side of the lens holder 2, wherein one lens may be a focusing lens and the rest may be translucent lenses. The focusing lens may be adjustable and reflect light. The reflection of the focusing lens may adjust the angle at which light incident on the lens holder 2 is emitted from the translucent lens.

[0066] In some embodiments of the present application, the debugging device includes a fixing component, which is used to fix the lens 21 of the lens holder 2. Figures 7 to 10 As shown, the fixing assembly may include a flexible member 41 and a pressing member 42 .

[0067] The pressing piece 42 is used to be detachably connected to the lens holder 2 to fix the lens 21 of the lens holder 2. The pressing piece 42 may include a plurality of light-through holes 44, which correspond one-to-one to the plurality of lenses 21. The lenses 21 can also be observed and adjusted through the light-through holes 44 to ensure accurate bonding of the lenses 21.

[0068] In the present application, the flexible member 41 can be used to fit the lens 21. The flexible member 41 can be a flexible member with a buffering effect such as silicone, plastic, or rubber.

[0069] During the assembly process of the lens holder 2, the lens 21 is first bonded to the lens holder bracket 22 using adhesive. The flexible member 41 is then attached to the lens 21. The flexible member 41 and the lens 21 are then compressed using the pressing member 42, sandwiching the flexible member between the pressing member and the lens, until the lens 21 is secured to the lens holder bracket 22. Specifically, if glue is used to bond the lens to the lens holder bracket, a fixing assembly is used to secure the lens until the glue cures.

[0070] In some embodiments of the present application, Figure 11 As shown, the lens debugging method may include the following steps for fixing the lens on the lens holder:

[0071] S11: pasting multiple lenses to the lens holder;

[0072] S12: attaching the flexible member to the lens;

[0073] S13: Fix the pressing member to the lens seat so that the flexible member is clamped between the pressing member and the lens seat until the lens is fixed to the lens seat.

[0074] In the present application, the flexible member 41 is sandwiched between the pressing member 42 and the plurality of lenses 21 , thereby reducing the pressure of the pressing member 42 on the lenses 21 and preventing damage to the lenses due to excessive pressure.

[0075] In some embodiments of the present application, the fixing assembly may further include a fastener 43, which is disposed on the pressing member 42 to fix the pressing member 42 to the lens holder 2. The fastener 43 may be one or more fasteners 43. The fastener 43 may be a screw, bolt, stud, or other component.

[0076] In some embodiments of the present application, the compression member 42 includes a first portion 401 and a second portion 402 connected to each other, with the first portion 401 and the second portion 402 extending in different directions. The flexible member 41 is sandwiched between the first portion 401 and the lens 21, and the fastener 43 can be disposed on the second portion 402 and fixed to the lens holder 2.

[0077] In the present application, the first portion 401 and the second portion 402 can be arranged perpendicular to each other. It should be noted that the extension direction of the first portion 401 and the second portion 402 can be determined according to the shape of the lens holder 2, in order to better fix the lens in the lens holder by applying pressure to the lens holder on both sides.

[0078] The first portion 401 and the second portion 402 extend in different directions, thereby enabling the lens to be fixed in different directions.

[0079] In some embodiments of the present application, Figure 7 and Figure 8 As shown, the pressing member 42 may include a first pressing member 421 and a second pressing member 422. The first pressing member 421 is used to secure the plurality of first lenses located on the first side of the lens holder 2. The first pressing member 421 includes a plurality of first light-through holes, which correspond one-to-one to the plurality of first lenses. The second pressing member 422 is used to secure the plurality of second lenses located on the second side of the lens holder 2. The second pressing member 422 includes a plurality of second light-through holes, which correspond one-to-one to the plurality of second lenses, with the first side and the second side facing each other.

[0080] In some embodiments of the present application, Figure 7 As shown, the flexible member 41 may include a first flexible member 411 and a second flexible member 412. The first flexible member 411 is used to adhere to the plurality of first lenses and is sandwiched between the first pressing member 421 and the plurality of first lenses. The second flexible member 412 is used to adhere to the plurality of second lenses and is sandwiched between the second pressing member 422 and the plurality of second lenses.

[0081] The first pressing member 421 may include a first portion 401 and a second portion 402 extending in different directions. The first flexible member 411 is sandwiched between the first portion 401 and the lens 21 . A plurality of fasteners 43 may be disposed on the second portion 402 and fixed to the lens holder 2 .

[0082] The second pressing member 422 may include a first portion 401 and a second portion 402 extending in different directions. The second flexible member 412 is sandwiched between the first portion 401 and the lens 21 . The fastener 43 may be disposed on the second portion 402 and fixed to the lens holder 2 .

[0083] The shapes of the second part 402 of the first pressing member 421 and the second part 402 of the second pressing member 422 can be different. In order to achieve a compact structure, the shapes of the second part 402 of the first pressing member 421 and the second part 402 of the second pressing member 422 can avoid each other as long as the effect of fixing the pressing member can be achieved.

[0084] In the present application, after the lens is fixed to the bracket of the lens seat by a fixing component to complete the installation of the lens seat, the lens seat can be placed in the base and debugged by the marking component. During the debugging process, the lens seat can be debugged by adjusting the focusing lens 2000 in the lens seat.

[0085] like Figure 5 and Figure 6 As shown, the marking assembly 20 may include a marking portion 201, which may include one or more concentric circle scales 203. Each concentric circle scale 203 may include multiple concentric circles 204 of varying diameters. The center of at least one of the multiple concentric circles 204 may be marked. For example, the center of the concentric circle may be marked with a cross, intersecting lines, a circle, or other regular or irregular pattern. Marking the center of the circle helps confirm whether the center of the spot formed by the light irradiating the marking portion overlaps with the center of the concentric circle.

[0086] In this application, if Figure 5 In the illustrated embodiment, the marking portion 201 of the marking assembly 20 has a concentric scale 203. The marking assembly 20 having a concentric scale 203 can be used to adjust a lens mount that emits a beam of light. In some embodiments, the marking assembly 20 having a concentric scale 203 can be used to adjust the light source assembly 3.

[0087] In this application, if Figure 6 In the illustrated embodiment, the marking portion 201 of the marking assembly 20 has a plurality of concentric circular scales 203. The marking assembly 20 having a plurality of concentric circular scales 203 can be used to debug a lens holder having a plurality of lenses emitting a plurality of light beams. Figure 6 Also shown is the light path L of the light incident on the marking component 20.

[0088] During the debugging process of the lens of the lens holder 2, the light emitted from the lens of the lens holder 2 is irradiated onto the marking portion 201. The tester can determine whether the lens 21 of the lens holder 2 is installed properly by observing whether the center of the light spot formed by the light irradiating the marking portion 201 overlaps with the center of the concentric circle.

[0089] The marking assembly 20 is movably mounted on the positioning portion 110. The marking assembly 20 can be relatively moved in the direction of light incident on the marking assembly to move away from or closer to the lens holder 2. When the marking assembly 20 is moved away from the lens holder 2, the light spot formed by the marking assembly 20 becomes larger, which facilitates clearer identification of the center of the light spot and thus facilitates comparison with the corresponding concentric circle 204. When the marking assembly 20 is moved away from the lens holder 2, the light spot formed by the marking assembly 20 becomes smaller, which facilitates clearer identification of the range of the light spot and thus facilitates determination of the concentric circle 204 for comparison with the light spot.

[0090] In this application, if the center of the spot formed by the light irradiating the marking assembly overlaps with the center of the concentric circle, the lens of the lens holder is determined to be qualified. If the center of the spot formed by the light irradiating the marking assembly overlaps with the center of the concentric circle, the lens of the lens holder is adjusted until the center of the spot formed by the light irradiating the marking assembly overlaps with the center of the concentric circle. For example, by adjusting the focusing lens 2000 on the lens holder 2, the center of the spot formed by the light irradiating the light from the transparent lens of the lens holder 2 onto the marking portion 201 of the marking assembly 20 can be adjusted to overlap with the center of the concentric circle 204.

[0091] In this application, if Figure 12 As shown, the lens debugging method may include the following steps:

[0092] S21: The debugging light is irradiated to the marking component through the lens of the lens holder.

[0093] S22: Move the marking component along the positioning portion to observe the overlapping range of the light spot and the concentric circle.

[0094] S23: The lens of the lens holder is determined to be qualified based on the fact that the center of the light spot formed by the light irradiating the marking component overlaps with the center of the concentric circle. The lens of the lens holder is adjusted until the center of the light spot formed by the light irradiating the marking component coincides with the center of the concentric circle based on the fact that the center of the light spot formed by the light irradiating the marking component does not overlap with the center of the concentric circle.

[0095] In some embodiments of the present application, the positioning portion 110 may include a guide mechanism 1100, and the marking assembly 20 may be disposed within and movable along the guide mechanism 1100. The guide mechanism provides guidance for the placement of the marking assembly, facilitating accurate placement of the marking assembly. The placement of the guide mechanism 1100 may be determined based on test requirements. Specifically, based on the spectral detection requirements of the flow cytometer, the guide mechanism 1100 is positioned at a target location to ensure that light emitted from the lens holder is irradiated at the target location. The target location may encompass a range and is not limited to a specific location.

[0096] In this application, if Figures 1 to 4 As shown, the positioning portion 110 can be a positioning groove, and a portion of the marking assembly 20 is located within the positioning groove and can move within the positioning groove. The guiding mechanism 1100 can be a groove wall of the positioning groove, and the marking assembly 20 is guided by the groove wall while sliding. The groove wall guides the marking assembly 20, which simplifies the structure and saves production costs and processes.

[0097] In the present application, the guide mechanism may be a guide track, for example, a track, a slide rail, etc. may be provided at the target position. Correspondingly, the marking assembly may have components such as pulleys and sliders to slide on the guide track.

[0098] In the present application, the guide mechanism may be a groove or a protrusion, and correspondingly, the marking component may have a protrusion or a groove to move along the aforementioned groove or protrusion.

[0099] It should be noted that the present application does not limit the guiding mechanism to the structure described in the aforementioned embodiment. The guiding mechanism of the present application may be any structure that can guide the movement of the marking component in the incident direction of light toward the marking component.

[0100] In some embodiments of the present application, Figure 5 and Figure 6 As shown, the marking assembly 20 may include a support portion 202, which is disposed on the positioning portion 110. The marking portion 201 stands upright on the support portion 202, and the marking portion 201 is perpendicular to the support portion 202. The support portion 202 may include a first limiting portion 210, and the positioning portion 110 may include a second limiting portion 120. The shapes of the second limiting portion 120 and the first limiting portion 210 may be adapted to limit the position of the support portion 202 relative to the lens holder 2 along the incident direction of light on the marking assembly 20.

[0101] Limiting the marking component to move in a direction close to or away from the lens seat is beneficial for the marking component to move along the incident direction of the light to the marking component to adjust the size of the light spot.

[0102] In some embodiments of the present application, Figures 1 to 4 As shown, the first limiting portion 210 may be a limiting groove formed in the support portion 202. The second limiting portion 120 may include a plurality of limiting protrusions arranged at intervals along the incident direction of the light toward the marking assembly 20, and the limiting groove may accommodate some of the plurality of limiting protrusions.

[0103] The multiple limiting protrusions are arranged at intervals, and the distance of movement of the marking assembly can be adjusted by the distance between the multiple limiting protrusions. Specifically, the multiple limiting protrusions are spaced at a certain distance, and during the movement of the marking assembly, the distance of movement of the marking assembly can be determined by the distance between the limiting protrusions located in the limiting groove.

[0104] In some embodiments, the target spot diameter A can be determined based on the spectral detection requirements of the flow cytometer when light is irradiated at a specific target location. During testing, the actual spot diameter B formed by the light emitted from the lens holder and irradiated onto the marker assembly is determined. The difference between diameters A and B can be used to determine the offset of the light path, and the light source assembly can be adjusted based on this offset. It should be noted that the adjustment of the light source assembly is based on the fact that the offset of the light is caused by the light emitted by the light source assembly. If other optical components in some flow cytometers may cause an offset in the light path, these components can also be adjusted accordingly. For example, if the target spot diameter formed at a certain location is 4.5 mm and the actual spot diameter at that location is 5 mm, the offset of the light path at that location is ±0.25. Accordingly, by moving the marker assembly along the guide mechanism to the desired position, light spots of different diameters and the offset of the light path at that location can be obtained.

[0105] In some embodiments of the present application, Figures 1 to 4 As shown, the marking assembly 20 may include a first marking assembly 211 and a second marking assembly 222 , each of which includes a plurality of marking portions.

[0106] In some embodiments of the present application, Figures 1 to 4 As shown, the positioning portion 110 may include a first positioning portion 111 and a second positioning portion 112, the first marking component 211 is detachably provided on the first positioning portion 111, and the second marking component 222 is detachably provided on the second positioning portion 112, and the lens seat mounting portion 130 includes a first lens seat mounting portion 131 for mounting the first lens seat 2001, and the first lens seat mounting portion 131 is located between the first positioning portion 111 and the second positioning portion 112, and the light emitted from the lenses on both sides of the first lens seat 2001 is respectively irradiated to the marking portion of the first marking component 211 and the marking portion of the second marking component 222.

[0107] The arrangement of the first positioning portion 111 and the second positioning portion 112 can facilitate adjustment of a lens in a lens holder capable of emitting light from both sides. In some embodiments, the first positioning portion 111 and the second positioning portion 112 are symmetrically arranged about the first lens holder 2001. However, the present application is not limited thereto. In some embodiments, the specific locations of the first and second positioning portions can be determined based on the optical path of light emitted from the first lens holder.

[0108] like Figures 1 to 4 As shown, the first marking assembly 211 can move along the first groove wall 1101 of the positioning groove of the first positioning portion 111, and the first groove wall 1101 serves as the guide mechanism 1100 for the first marking assembly 211. The second marking assembly 222 can move along the second groove wall 1102 of the positioning groove of the second positioning portion 112, and the second groove wall 1102 serves as the guide mechanism 1100 for the second marking assembly 222.

[0109] In some embodiments of the present application, the base includes a first light source mounting portion 141, and the first light source mounting portion 141 is used to mount the light source assembly 3. The marking assembly 20 may further include a third marking assembly 233, which is detachably disposed on the first positioning portion 111. The third marking assembly 233 may be as follows: Figure 5 The third marking component 233 can be used to debug the light source component 3.

[0110] like Figures 1 to 4 As shown, the third marking assembly 233 can move along the third groove wall 1103 of the positioning groove of the first positioning portion 111 , and the third groove wall 1103 serves as a guide mechanism 1100 for the third marking assembly 233 .

[0111] In the present application, along the incident direction of the light to the third marking component 233, the first light source mounting portion 141 is located on the upstream side of the third marking component 233, and the light emitted from the light source component 3 is irradiated to the third marking component 233. The posture of the light source component is adjusted according to the positional relationship between the light spot formed by the light emitted from the light source component 3 to the marking part of the third marking component 233 and the concentric circles in the concentric circle scale.

[0112] Providing the third marking component 233 is beneficial for adjusting the posture of the light source component 3 to improve the yield rate of the light source component 3 .

[0113] The third marking assembly 233 can be placed on the first positioning portion 111, making full use of the first positioning portion 111, saving space in the base and facilitating miniaturization of the base. After the light source assembly 3 is tested and found to be qualified using the third marking assembly 233, the third marking assembly 233 is removed from the first positioning portion 111 to allow the lens holder 2 to be tested.

[0114] In this application, the third marking component 233 may also be referred to as a light source marking component. The third marking component 233 may be as follows: Figure 5 A marking assembly is shown having one marking portion.

[0115] In some embodiments of the present application, Figure 13 As shown, the lens debugging method also includes the following steps of debugging the light source assembly:

[0116] S31: The debugging light emitted by the light source assembly is irradiated onto the light source marking assembly.

[0117] S32: Move the light source marking assembly along the positioning portion to observe the overlapping range of the light spot and the concentric circle.

[0118] S33: Determine whether the light source assembly is qualified based on the fact that the center of the light spot formed by the light irradiating the light source marking assembly overlaps with the center of the concentric circle of the light source marking assembly; adjust the light emitted by the light source assembly until the center of the light spot formed by the light irradiating the light source marking assembly overlaps with the center of the concentric circle of the light source marking assembly based on the fact that the center of the light spot formed by the light irradiating the light source marking assembly does not overlap with the center of the concentric circle of the light source marking assembly.

[0119] The method of the present application can be used to test and adjust the light source assembly so that the angle of the light emitted by the light source assembly meets the requirements of the flow cytometer.

[0120] In some embodiments of the present application, the base 10 may further include a light source testing portion 100, which may include a fifth positioning portion 115 and a second light source mounting portion 142. The light source testing portion 100 may be located at an edge of the base 10. The second light source mounting portion 142 is used to mount the light source assembly 3, and the second light source mounting portion 142 is disposed opposite the fifth positioning portion 115.

[0121] In some embodiments of the present application, the third marking component 233 is detachably disposed on the fifth positioning portion 115, and the posture of the light source component 3 is adjusted according to the positional relationship between the light spot formed by the light emitted from the light source component 3 and the marking portion of the third marking component 233 and the concentric circles in the concentric circle scale.

[0122] In some embodiments of the present application, the light source assembly 3 can be debugged in the light source testing section 100 through the third marking assembly 233. After the light source assembly 3 passes the debugging, the light source assembly 3 is moved to the first light source installation section 141 to debug the lens holder 2.

[0123] The light source testing part 100 is located at the edge of the base 10. On the one hand, the light source assembly 3 can be debugged. On the other hand, the light source assembly 3 can be placed on the first light source mounting part 141 after debugging the light source assembly 3 at the light source testing part 100. Compared with debugging the light source assembly at the first light source mounting part and then continuing to debug the lens seat, this method can improve efficiency.

[0124] In some embodiments of the present application, Figures 2 to 4 As shown, the debugging device may include a spectroscopic component 11, which can be arranged opposite to the first light source mounting portion 141. Along the incident direction of the light to the mounting portion of the first lens seat 2001, the spectroscopic component 11 is located on the upstream side of the mounting portion 131 of the first lens seat. The light emitted by the light source component 3 passes through the spectroscopic component 11 and then emits as a first light and a second light. The first light passes through the lens of the first lens seat and then emits the light that is emitted to the marking portion of the first marking component 211 and the marking portion of the second marking component 222 respectively. The posture of the lens of the first lens seat 2001 is tested and adjusted according to the positional relationship between the light spot formed by the first light irradiated to the marking portion of the first marking component 211 and the marking portion of the second marking component 222 and the concentric circles in the concentric circle scale.

[0125] In some embodiments of the present application, Figures 2 to 4 As shown, the marking assembly 20 may include a fourth marking assembly 244 and a fifth marking assembly 255, each of which includes a plurality of marking portions. The fourth marking assembly 244 and the fifth marking assembly 255 may be as follows: Figure 6 A marking assembly is shown having multiple marking portions.

[0126] In some embodiments of the present application, Figures 2 to 4 As shown, the positioning portion 110 may include a third positioning portion 113 and a fourth positioning portion 114, the fourth marking component 244 is detachably disposed on the third positioning portion 113, and the fifth marking component 255 is detachably disposed on the fourth positioning portion 114. The lens seat mounting portion 130 includes a second lens seat mounting portion 132 for mounting the second lens seat 2002. The second lens seat 2002 is disposed between the third positioning portion 113 and the fourth positioning portion 114. Along the incident direction of the light toward the second lens seat mounting portion 132, the light splitting group Component 11 is located on the upstream side of the second lens holder mounting portion 132. The second light emitted from the spectroscopic component 11 passes through the lenses on both sides of the second lens holder 2002 and then irradiates the marking portion of the fourth marking component 244 and the marking portion of the fifth marking component 255 respectively. The posture of the lens of the second lens holder 2002 is tested and adjusted based on the positional relationship between the light spot formed by the second light irradiating the marking portion of the fourth marking component 244 and the marking portion of the fifth marking component 255 and the concentric circles in the concentric circle scale.

[0127] By using the light splitting component 11 to split the light emitted by the light source component into the first light and the second light, the installation of the lenses of the first lens seat 2001 and the second lens seat 2002 can be detected and adjusted at the same time, thereby improving the efficiency of debugging the lenses.

[0128] In addition, in some flow cytometers, the light from the light source assembly is divided into two beams of light through a spectroscopic element to pass through two lens holders for testing. Such a setting in the present application is conducive to simulating real situations, thereby improving the accuracy of the lenses of the lens holder in actual use.

[0129] like Figures 2 to 4 As shown, the fourth marking assembly 244 can move along the fourth groove wall 1104 of the positioning groove of the third positioning portion 113, and the fourth groove wall 1104 serves as the guide mechanism 1100 for the fourth marking assembly 244. The fifth marking assembly 255 can move along the fifth groove wall 1105 of the positioning groove of the fifth positioning portion 115, and the fifth groove wall 1105 serves as the guide mechanism 1100 for the fifth marking assembly 255.

[0130] In some embodiments of the present application, Figures 2 to 4 As shown, the debugging device may include a first refractive component 151 and a second refractive component 152. Along the incident direction of the first light ray toward the mounting portion of the first lens holder 2001, the first refractive component 151 is located on the upstream side of the first lens holder mounting portion 131 and between the beam splitting component 111 and the first lens holder mounting portion 131. The first light ray emitted from the beam splitting component 11 is incident on the first refractive component 151 and is refracted by the first refractive component 151 before being incident on the first lens holder 2001.

[0131] In some embodiments of the present application, Figures 2 to 4 As shown, along the incident direction of the second light ray toward the second lens seat mounting portion 132, the second refractive component 152 is located on the upstream side of the second lens seat mounting portion 132 and is located between the spectroscopic component 11 and the second lens seat mounting portion 132. The second light ray emitted from the spectroscopic component 11 is incident on the second refractive component 152 and is refracted by the second refractive component 152 before being incident on the second lens seat 2002.

[0132] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0133] In the description of the embodiments of this application, the technical terms "first," "second," "third," etc. are used only to distinguish different objects and should not be understood to indicate or imply relative importance or implicitly specify the quantity, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, the meaning of "plurality" is more than two, unless otherwise specifically defined.

[0134] References herein to "embodiments" mean that a particular feature, structure, or characteristic described in connection with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor does it constitute an independent or alternative embodiment that is mutually exclusive of other embodiments. It is understood, both explicitly and implicitly, by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0135] In the description of the embodiments of this application, the term "and / or" is simply a description of the association relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can represent the following three situations: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0136] In the description of the embodiments of the present application, the orientations or positional relationships indicated by technical terms such as "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", and "circumferential" are based on the orientations or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed, operated or used in a specific orientation. Therefore, they should not be understood as limitations on the embodiments of the present application.

[0137] In the description of the embodiments of the present application, unless otherwise expressly specified or limited, technical terms such as "installed," "connected," "connect," and "fixed" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and they can refer to internal communication between two components or interaction between two components. Those skilled in the art can understand the specific meanings of the above terms in the embodiments of the present application based on specific circumstances.

[0138] In the description of the embodiments of the present application, unless otherwise clearly specified and limited, the technical term "contact" should be understood in a broad sense, and can be direct contact, contact through an intermediate medium layer, contact with essentially no interaction force between the two contacting parties, or contact with interaction force between the two contacting parties.

[0139] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some or all of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present application, and they should all be included in the scope of the claims and specification of the present application. In particular, as long as there is no structural conflict, the various technical features mentioned in the various embodiments can be combined in any way. The present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions that fall within the scope of the claims.

Claims

1. A debugging device, characterized in that: Used for debugging a lens installed in a lens holder, the debugging device comprises: The base comprises a positioning portion and a lens seat mounting portion, wherein the lens seat mounting portion is used to mount the lens seat; A marking assembly is movably mounted on the positioning portion, wherein the marking assembly includes a marking portion, and the marking portion includes one or more arranged concentric circle scales, each of the concentric circle scales includes a plurality of concentric circles with different diameters. Among them, along the incident direction of the light to the marking assembly, the lens seat mounting portion is located on the upstream side of the marking assembly, and the posture of the lens is adjusted according to the positional relationship between the light spot formed by the light passing through the lens and irradiating the marking portion and the concentric circles in the concentric circle scale.

2. The debugging device according to claim 1, characterized in that: The positioning portion has a guide mechanism, and the marking component is arranged on the guide mechanism and is movable along the guide mechanism.

3. The debugging device according to claim 1, wherein: The marking assembly includes a supporting portion, the supporting portion is arranged on the positioning portion, the marking portion is erected on the supporting portion, and the supporting portion includes a first limiting portion; The positioning portion includes a second limiting portion, which is adapted to the first limiting portion to limit the position of the supporting portion relative to the lens seat along the incident direction of the light to the marking assembly.

4. The debugging device according to claim 3, characterized in that: The first limiting portion is a limiting groove; The second limiting portion includes a plurality of limiting protrusions, which are arranged at intervals along the incident direction of light to the marking component, and the limiting groove accommodates some of the plurality of limiting protrusions.

5. The debugging device according to claim 2, characterized in that: The marking assembly includes a first marking assembly and a second marking assembly, wherein the first marking assembly and the second marking assembly each include a plurality of marking portions; The positioning portion includes a first positioning portion and a second positioning portion, the first marking assembly is detachably mounted on the first positioning portion and is movable relative to the first positioning portion, and the second marking assembly is detachably mounted on the second positioning portion and is movable relative to the second positioning portion. The lens seat mounting portion includes a first lens seat mounting portion for mounting a first lens seat, the first lens seat mounting portion is located between the first positioning portion and the second positioning portion, and the light emitted from the lenses on both sides of the first lens seat is respectively irradiated to the marking portion of the first marking assembly and the marking portion of the second marking assembly.

6. The debugging device according to claim 5, characterized in that: The base includes a first light source mounting portion, and the first light source mounting portion is used to mount the light source assembly; The marking assembly also includes a third marking assembly, which is detachably arranged on the first positioning portion. Along the incident direction of the light to the third marking assembly, the first light source mounting portion is located on the upstream side of the third marking assembly. The positional relationship between the light spot formed by the light emitted from the light source assembly to the marking portion of the third marking assembly and the concentric circles in the concentric circle scale of the first marking assembly is used to adjust the posture of the light source assembly.

7. The debugging device according to claim 6, characterized in that: The debugging device includes a spectroscopic assembly, which is arranged opposite to the first light source mounting portion. Along the incident direction of the light to the first lens holder mounting portion, the spectroscopic assembly is located on the upstream side of the first lens holder mounting portion. The light emitted by the light source assembly is emitted as a first light and a second light after passing through the spectroscopic assembly. The first light passes through the lens of the first lens holder and then emits the light that is emitted to the marking portion of the first marking assembly and the marking portion of the second marking assembly respectively. The posture of the lens of the first lens holder is tested, adjusted and debugged based on the positional relationship between the light spot formed by the first light irradiated to the marking portion of the first marking assembly and the marking portion of the second marking assembly and the concentric circles in the concentric circle scale; The marking assembly includes a fourth marking assembly and a fifth marking assembly, each of the fourth marking assembly and the fifth marking assembly includes a plurality of marking parts; The positioning portion includes a third positioning portion and a fourth positioning portion, the fourth marking assembly is detachably mounted on the third positioning portion and is movable relative to the third positioning portion, and the fifth marking assembly is detachably mounted on the fourth positioning portion and is movable relative to the fourth positioning portion. The lens seat mounting portion includes a second lens seat mounting portion for mounting a second lens seat, and the second lens seat is arranged between the third positioning portion and the fourth positioning portion. Along the incident light path of the light to the second lens seat mounting portion, the spectroscopic component is located on the upstream side of the second lens seat mounting portion. The second light emitted from the spectroscopic component passes through the lenses on both sides of the second lens seat and then the light emitted is respectively irradiated to the marking portion of the fourth marking component and the marking portion of the fifth marking component. The posture of the lens of the second lens seat is tested, adjusted and debugged according to the positional relationship between the light spot formed by the second light irradiated to the marking portion of the fourth marking component and the marking portion of the fifth marking component and the concentric circles in the concentric circle scale.

8. The debugging device according to claim 7, characterized in that: The debugging device includes a first refraction component and a second refraction component; Along the incident direction of the first light ray toward the first lens holder mounting portion, the first refractive component is located on the upstream side of the first lens holder mounting portion and between the beam splitting component and the first lens holder mounting portion, and the first light ray emitted from the beam splitting component is incident on the first refractive component and is refracted by the first refractive component before being incident on the first lens holder; Along the incident direction of the second light ray toward the second lens seat mounting portion, the second refractive component is located on the upstream side of the second lens seat mounting portion and between the beam splitting component and the second lens seat mounting portion. The second light ray emitted from the beam splitting component is incident on the second refractive component and is refracted by the second refractive component before being incident on the second lens seat.

9. The debugging device according to claim 6, characterized in that: The base includes a light source testing portion, which includes a fifth positioning portion and a second light source mounting portion. The light source testing portion is located at an edge of the base, and the second light source mounting portion is used to mount the light source assembly. The second light source mounting portion is arranged opposite to the fifth positioning portion. The third marking component is detachably arranged on the fifth positioning portion, and the posture of the light source component is adjusted according to the positional relationship between the light spot formed by the light emitted from the light source component installed on the second light source installation portion and the light irradiated on the marking portion of the third marking component and the concentric circles in the concentric circle scale of the third marking component.

10. The debugging device according to any one of claims 1 to 9, characterized in that: The debugging device includes a fixing assembly, which is used to fix the lens of the lens holder, and the fixing assembly includes: A pressing member, used for detachably connecting with the lens holder to fix the lens of the lens holder, the pressing member comprising a plurality of light-through holes, the plurality of light-through holes corresponding one-to-one to the plurality of lenses; The flexible member is used to fit the plurality of lenses, and the flexible member is sandwiched between the pressing member and the plurality of lenses.

11. The debugging device according to claim 10, characterized in that: The fixing assembly includes a fastener, which is arranged on the pressing member to fix the pressing member to the lens seat.

12. The debugging device according to claim 11, characterized in that: The pressing member includes a first part and a second part connected to each other, the first part and the second part extend in different directions respectively, the flexible member is clamped between the first part and the lens, and the fastener is arranged in the second part and fixed to the lens seat.

13. The debugging device according to claim 10, characterized in that: The pressing member comprises: a first pressing member, for fixing the plurality of first lenses located on the first side of the lens holder, wherein the first pressing member comprises a plurality of first light-through holes, and the plurality of first light-through holes correspond one-to-one to the plurality of first lenses; a second pressing member for fixing the plurality of second lenses located on the second side of the lens holder, the second pressing member comprising a plurality of second light-through holes, the plurality of second light-through holes corresponding one-to-one to the plurality of second lenses, the first side being opposite to the second side; The flexible member comprises: a first flexible member, used for being attached to the plurality of first lenses, wherein the first flexible member is sandwiched between the first pressing member and the plurality of first lenses; The second flexible member is used to fit the plurality of second lenses, and the second flexible member is sandwiched between the second pressing member and the plurality of second lenses.

14. A lens debugging method, characterized in that: The method comprises: The debugging light is irradiated to the marking component through the lens of the lens holder; The marking assembly is moved along the positioning portion to observe the overlapping range of the light spot and the concentric circles, wherein the marking assembly includes one or more marking portions, each of the marking portions includes a plurality of arranged concentric circle scales of different diameters, and each of the concentric circle scales includes a plurality of concentric circles of different diameters; The lens of the lens holder is determined to be qualified based on the fact that the center of the light spot formed by the light irradiating the marking component overlaps with the center of the concentric circle. The lens of the lens holder is adjusted until the center of the light spot formed by the light irradiating the marking component coincides with the center of the concentric circle based on the fact that the center of the light spot formed by the light irradiating the marking component does not overlap with the center of the concentric circle.

15. The lens debugging method according to claim 14, characterized in that: The marking assembly includes a light source marking assembly, and the method further includes: The debugging light emitted by the light source assembly is irradiated onto the light source marking assembly; Moving the light source marking assembly along the positioning portion to observe the overlapping range of the light spot and the concentric circle; The light source assembly is determined to be qualified based on the fact that the center of the light spot formed by the light irradiating the light source marking assembly overlaps with the center of the concentric circle of the light source marking assembly. Based on the fact that the center of the light spot formed by the light irradiating the light source marking assembly does not overlap with the center of the concentric circle of the light source marking assembly, the light emitted by the light source assembly is adjusted until the center of the light spot formed by the light irradiating the light source marking assembly overlaps with the center of the concentric circle of the light source marking assembly.

16. The lens debugging method according to claim 14, characterized in that: The method further comprises: affixing a plurality of lenses to the lens holder; attaching a flexible member to the lens; The pressing member is fixed to the lens seat so that the flexible member is clamped between the pressing member and the lens seat until the lens is fixed to the lens seat.

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