Cracking and amplifying integrated nucleic acid detector

Abstract

The invention discloses a pyrolysis and amplification integrated nucleic acid detector, and belongs to the technical field of nucleic acid detection.The pyrolysis and amplification integrated nucleic acid detector comprises a shading shell, a pyrolysis module and an amplification analysis module are installed in the shading shell, the pyrolysis module comprises a pyrolysis pipe and a heating device, and a heating opening is formed in the heating device; the amplification analysis module comprises an amplification tube, a constant temperature device and a spectral analysis device, the top of the shading shell is provided with an extending opening and a containing groove, a tube opening of the cracking tube extends out through the extending opening, a sealing cover is arranged at a groove opening of the containing groove, an embedding opening is formed in the groove bottom of the containing groove, and an inserting opening is formed in the constant temperature device. According to the method, the split liquid is directly poured into the amplification tube from the splitting tube for amplification and spectral analysis, and a paramagnetic particle method is not needed for transfer and purification, so that the infection risk in the transfer process can be reduced, the transfer frequency is reduced, the washing and elution steps are omitted, the detection steps are simplified, and the detection efficiency is improved. And the detection efficiency and the detection precision are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a nucleic acid detector integrating lysis and amplification. Background Art

[0002] Current nucleic acid testing methods primarily utilize magnetic bead-based methods for sample lysis and washing, followed by PCR (Polymerase Chain Reaction) amplification and display of results. The magnetic bead-based method is a nucleic acid extraction technology that utilizes nano-biological magnetic beads as carriers. Through the specific recognition and binding of silanol groups on the bead surface to nucleic acid molecules, it aggregates or disperses nucleic acids under the influence of an external magnetic field, achieving automated extraction. PCR amplification, in particular, simulates the natural DNA replication process through temperature control, cyclically amplifying target DNA fragments and achieving exponential growth.

[0003] The main process is as follows: S1. Inject the sample and lysis solution into the lysis tube for lysis to release DNA or RNA; S2. Add magnetic beads to the lysis tube and adjust the temperature conditions to allow the DNA or RNA to bind to the magnetic beads; S3. Aspirate the magnetic beads and place them in a washing tube containing washing solution to wash away impurities on the magnetic beads; S4. Aspirate the magnetic beads and place them in an elution tube containing elution solution to elute the DNA or RNA from the magnetic beads; S5. Send them to the PCR amplifier for amplification and display the results.

[0004] However, the above method has many steps and complicated operations, and requires conversion between different functional instruments. The magnetic beads are easily contaminated during the conversion process, and the conversion process will prolong the test time, resulting in insufficient detection speed and accuracy. Summary of the Invention

[0005] The purpose of the present invention is to solve the above-mentioned technical problems and provide an integrated nucleic acid detector for lysis and amplification. In the integrated nucleic acid detector for lysis and amplification of the present invention, a method of pouring the lysed liquid from the lysis tube directly into the amplification tube for amplification and spectral analysis is adopted. Compared with the PCR amplifier, the spectral analysis method has strong anti-interference ability for the analysis of nucleic acid information. Sample lysis does not require the use of a magnetic bead method for transfer and purification, which not only reduces the risk of infection during the transfer process, but also reduces the number of transfers, eliminates the washing and elution steps, simplifies the detection steps, and improves the detection efficiency and accuracy.

[0006] To achieve the above-mentioned purpose, the present invention provides the following solution: The present invention discloses an integrated nucleic acid detector for lysis and amplification, comprising a light-shielding shell, in which a lysis module and an amplification analysis module are installed, the lysis module comprising a lysis tube and a heating device, the heating device being provided with a heating port for plugging and unplugging the lysis tube, the amplification analysis module comprising an amplification tube, a constant temperature device for providing a constant temperature environment for the amplification tube, and a spectral analysis device for performing spectral analysis on the sample in the amplification tube, the top of the light-shielding shell being provided with a protruding port and a receiving groove, the protruding port being coaxially arranged above the heating port, the tube mouth of the lysis tube being extended through the protruding port, the groove of the receiving groove being provided with a closing cover, the groove bottom of the receiving groove being provided with an embedding port, the embedding port being provided for the upper half of the amplification tube to extend into the receiving groove, the constant temperature device being provided with an insertion port, the insertion port being provided for the lower half of the amplification tube to be plugged and unplugged.

[0007] Preferably, the tube cover of the lysis tube is provided with a tube cap, and the tube mouth of the amplification tube is provided with a sealing plug.

[0008] Preferably, the heating device includes a heating seat, a cracking heating film and a cracking temperature sensor, the heating port is located on the heating seat, the cracking heating film is arranged on the inner wall of the heating port, the cracking temperature sensor is arranged in the heating seat, and the cracking temperature sensor is used to monitor the temperature of the cracking heating film.

[0009] Preferably, the constant temperature device includes an amplification base, an amplification heating film and an amplification temperature sensor, the insertion port is arranged on the top wall of the amplification base, the side wall of the amplification base is provided with an incident port connected to the insertion port, the bottom wall of the amplification base is provided with a detection port connected to the insertion port, the incident end and the receiving end of the amplification spectral analysis device correspond to the incident port and the detection port respectively, the amplification heating film is attached to the surface of the amplification base, and the amplification temperature sensor is used to monitor the temperature of the amplification heating film.

[0010] Preferably, the amplification spectrum analysis device includes an LED light group and a spectrum sensor, the spectrum sensor corresponds to the detection port and is located below the detection port, and the LED light group includes a blue LED light, a yellow LED light and a red LED light; three said incident ports are provided on the amplification base, and the three said incident ports are arranged at three positions of the amplification tube, and the three said incident ports are all installed with filters, and the three said incident ports correspond to the blue LED light, the yellow LED light and the red LED light respectively.

[0011] Preferably, the closing cover is hinged on the notch of the accommodating groove, and the notch of the accommodating groove has a wall thickness capable of supporting the closing cover.

[0012] Preferably, the closing cover and the receiving slot are connected by a spring-pressed latch, and the spring-pressed latch includes a lock tongue and a lock tongue seat. One end of the closing cover is hinged to one end of the notch of the receiving slot, the lock tongue is arranged at the other end of the closing cover, and the lock tongue seat is arranged on the other end of the notch of the receiving slot.

[0013] Preferably, the amplification spectrum analysis device further includes a constant temperature amplification spectrum circuit board, and the amplification heating film, the amplification temperature sensor, the blue LED lamp, the yellow LED lamp and the red LED lamp are all electrically connected to the constant temperature amplification spectrum circuit board.

[0014] Preferably, a display screen is provided on the light-shielding shell, a control integrated circuit board is provided in the light-shielding shell, the heating device, the display screen and the constant temperature amplification spectrum circuit board are all electrically connected to the control integrated circuit board, and the display screen can display the detection results.

[0015] Preferably, a power supply interface is provided on the light-shielding shell.

[0016] Preferably, the power supply interface is a USB-C port.

[0017] Preferably, a heat dissipation hole is provided on the light-shielding shell, a heat dissipation fan is provided in the light-shielding shell, and the heat dissipation fan is electrically connected to the control integrated circuit board.

[0018] Preferably, the light-shielding shell includes a lower light-shielding shell and an upper light-shielding shell, the upper light-shielding shell is detachably connected to the lower light-shielding shell, the heat dissipation holes, the power supply interface, the display screen, the extension port and the notch of the accommodating slot are all arranged on the upper light-shielding shell, and the lysis module and the amplification analysis module are both installed on the lower light-shielding shell.

[0019] Preferably, a non-slip rubber pad is provided on the bottom of the lower shell of the light-shielding shell.

[0020] Compared with the prior art, the present invention has achieved the following technical effects: In the integrated nucleic acid detector for lysis and amplification of the present invention, a method is adopted in which the lysed liquid is poured directly from the lysis tube into the amplification tube for amplification and spectral analysis. Compared with the PCR amplifier, the spectral analysis method has strong anti-interference ability for the analysis of nucleic acid information. Sample lysis does not require the use of magnetic beads for transfer and purification, which not only reduces the risk of infection during the transfer process, but also reduces the number of transfers, eliminates the washing and elution steps, simplifies the detection steps, and improves the detection efficiency and accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly describes the drawings required for the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. Those skilled in the art can also derive other drawings based on these drawings without inventive effort. The "integrated nucleic acid detector" in the accompanying description is an abbreviation for an integrated nucleic acid detector for lysis and amplification.

[0022] Figure 1 This is a schematic diagram of the top-down stereoscopic structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 2 This is a schematic diagram of the three-dimensional structure of the integrated nucleic acid detector in an embodiment of the present invention when viewed from above; Figure 3 This is a schematic diagram of the front structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 4 This is a schematic diagram of the rear view structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 5 This is a schematic diagram of the front left perspective structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 6 This is a schematic diagram of the internal front left perspective structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 7 This is a schematic diagram of the internal rear left perspective structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 8 This is a schematic diagram of the internal rear right view of the integrated nucleic acid detector in an embodiment of the present invention; Figure 9 This is a schematic diagram of the internal front right structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 10 This is a schematic diagram of the internal left-side structure of the integrated nucleic acid detector in an embodiment of the present invention; Figure 11 This is a structural diagram of the internal cover plate of the integrated nucleic acid detector in an embodiment of the present invention; Figure 12 This is a schematic diagram of the structure of the amplification analysis module in an embodiment of the present invention; Figure 13 Schematic diagram of the positional relationship among the amplification base, LED light assembly, and amplification tube in an embodiment of the present invention; Figure 14 Schematic diagram of the positional relationship among the amplification base, LED light assembly, and amplification tube in an embodiment of the present invention; Figure 15 Schematic diagram of the cross-sectional structure of the amplification base, the three-color lamp holder and the amplification tube in an embodiment of the present invention; Figure 16Schematic diagram of the cross-sectional structure of the amplification base and the three-color lamp holder in an embodiment of the present invention; Figure 17 Schematic diagram of the structure of the upper shell of the light-shielding shell in an embodiment of the present invention; Figure 18 Schematic diagram of control logic in an embodiment of the present invention.

[0023] Explanation of the accompanying drawings: 1. Upper shell of the light-shielding shell; 2. Lower shell of the light-shielding shell; 3. Anti-slip rubber pad; 4. Display screen; 5. Heat dissipation hole; 6. Power supply interface; 7. Closing cover; 8. Silicone pad; 9. Receiving groove; 10. Lock tongue; 11. Lock tongue seat; 12. Control integrated circuit board; 13. Cracking tube; 14. Tube cap; 15. Mounting frame; 16. Heating device; 17. Cooling fan; 18. Three-color lamp holder; 19. Amplification tube; 20. Sealing plug; 21. Amplification base; 22. Amplification heating film; 23. Amplification temperature sensor; 24. Insertion port; 25. Incident port; 26. Detection port; 27. Constant temperature amplification spectrum circuit board; 28. Red LED; 29. ​​Yellow LED; 30. Blue LED; 31. Filter; 32. Spectral sensor; 33. Screw hole; 34. Extension port; 35. Embedded port; 36. Installation port. DETAILED DESCRIPTION

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments analyzed and obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0025] The purpose of the present invention is to provide an integrated nucleic acid detector for lysis and amplification to solve the problems existing in the prior art. In this integrated nucleic acid detector for lysis and amplification, a method is adopted in which the lysed liquid is poured directly from the lysis tube into the amplification tube for amplification and spectral analysis. Compared with the PCR amplifier, the spectral analysis method has strong anti-interference ability for the analysis of nucleic acid information. Therefore, the sample lysis does not need to be transferred and purified by the magnetic bead method, thereby reducing the risk of infection during the transfer process, reducing the number of transfers, eliminating the washing and elution steps, simplifying the detection steps, and improving the detection efficiency. In addition, the lysis and amplification are integrated in one nucleic acid detector, which also improves the detection efficiency.

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0027] like Figures 1 to 18As shown, this embodiment provides an integrated nucleic acid detector for lysis and amplification, including a light-shielding shell. A lysis module and an amplification analysis module are installed in the light-shielding shell. The lysis module includes a lysis tube 13 and a heating device 16, and the heating device 16 is provided with a heating port for plugging and unplugging the lysis tube 13. The amplification analysis module includes an amplification tube 19, a constant temperature device, and a spectral analysis device. The constant temperature device is used to provide a constant temperature environment for the amplification tube 19. The spectral analysis device is used to perform spectral analysis on the sample in the amplification tube 19. The top of the light-shielding shell is provided with an extension port 34 and a receiving groove 9, and the extension port 34 is connected to the light-shielding shell. The extension port 34 is coaxially arranged above the heating port, and the tube mouth of the lysis tube 13 extends through the extension port 34 to facilitate the injection of samples and lysis solution, as well as the plugging and unplugging of the lysis tube 13. A closing cover 7 is provided on the groove of the receiving groove 9, and an insertion port 35 is provided on the bottom of the receiving groove 9. The insertion port 35 allows the upper half of the amplification tube 19 to extend into the receiving groove 9. The constant temperature device is provided with an insertion port 24. The insertion port 24 is for inserting and removing the lower half of the amplification tube 19. Opening the sealing cap 7 can expose the tube opening of the amplification tube 19, so that the sample in the lysis tube 13 can be poured into the amplification tube 19. After the sealing cap 7 is closed, the light-shielding shell is placed in a dark room environment, which can prevent the amplification tube 19 from being affected by external light sources.

[0028] Working principle: Step 1: inject the sample and lysis solution into the lysis tube 13, and adjust the temperature in the lysis tube 13 to the lysis temperature through the heating port of the heating device 16. Under the action of the lysis solution, the sample will release DNA or RNA; Step 2: After the lysis is completed (after reaching the preset lysis time, such as a time setting of 5 minutes), the sealing cover 7 is opened to expose the tube mouth of the amplification tube 19, the lysis tube 13 is pulled out from the heating port and the extension port 34, and the liquid (lysate and sample) in the lysis tube 13 is poured into the amplification tube 19. The sealing cover 7 is closed to ensure that the sample is in a dark room environment. The constant temperature device adjusts the temperature in the amplification tube 19 to the amplification temperature, and the DNA or RNA is amplified; Step 3: After the amplification is completed (after the preset amplification time is reached), the spectrum analysis device performs spectrum analysis and records on the sample in the amplification tube 19, or the spectrum analysis device performs spectrum analysis and records from the beginning to the end of amplification.

[0029] The specific methods and sequence of the above steps are for reference only. You do not need to strictly follow the above steps and sequence, and you can adjust them according to the actual situation.

[0030] In this integrated lysis and amplification nucleic acid detector, the lysis liquid is poured directly from the lysis tube 13 into the amplification tube 19 for amplification and spectral analysis. Taking advantage of the strong anti-interference ability of the spectral analysis method, the sample lysis does not need to be transferred and purified by the magnetic bead method, thereby reducing the risk of infection during the transfer process. At the same time, the number of transfers is reduced, the washing and elution steps are eliminated, the detection steps are simplified, and the detection efficiency is improved. In addition, the lysis and amplification are integrated in a nucleic acid detector, which also improves the detection efficiency.

[0031] In one embodiment, the lysis tube 13 is provided with a cap 14 at its mouth. After the sample and lysis solution are injected, the cap 14 is placed on the mouth of the lysis tube 13 to prevent external contamination. Similarly, the amplification tube 19 is provided with a sealing plug 20 at its mouth. After the sample is injected, the sealing plug 20 is placed on the mouth of the amplification tube 19 to prevent external contamination.

[0032] In one embodiment, the heating device 16 in the pyrolysis module includes a heating base, a pyrolysis heating membrane, and a pyrolysis temperature sensor. The heating port is located on the heating base, and the pyrolysis heating membrane is mounted on the inner wall of the heating port (possibly using a surface mount method). A first sensor mounting hole is provided within the heating base, and the pyrolysis temperature sensor is mounted within the first sensor mounting hole. The pyrolysis temperature sensor is used to monitor the temperature of the heating base to regulate the heating temperature of the pyrolysis heating membrane and maintain the temperature within the pyrolysis tube 13 at a predetermined pyrolysis temperature.

[0033] In one embodiment, the heating seat in the cracking module is installed in the light-shielding shell of the light-shielding shell through the mounting bracket 15. The mounting bracket 15 supports the heating seat to have a sufficient height so that the tube mouth of the cracking tube 13 can extend out of the extension port 34.

[0034] In one embodiment, the extension port 34 is a stepped hole, and an annular boss is coaxially provided on the outer wall of the cracking tube 13. After the cracking tube 13 is inserted into the small aperture section of the stepped hole, the annular boss will be stuck in the large aperture section of the stepped hole, limiting the cracking tube 13. At this time, the bottom of the cracking tube 13 is just inserted into the heating port of the heating device 16. The insertion depth of the cracking tube 13 is set as needed, and the setting method includes but is not limited to adjusting the depth of the heating port, the height position of the heating port, and the distance between the annular boss and the tube mouth of the cracking tube 13.

[0035] In one embodiment, the constant temperature device in the amplification analysis module includes an amplification base 21, an amplification heating film 22, and an amplification temperature sensor 23. The insertion port 24 is provided on the top wall of the amplification base 21, and the insertion port 24 is for plugging and unplugging the lower half of the amplification tube 19. The side wall of the amplification base 21 is provided with an incident port 25, and the incident port 25 is connected to the insertion port 24. The bottom wall of the amplification base 21 is provided with a detection port 26, and the detection port 26 is connected to the insertion port 24. The transmitting end of the amplification spectral analysis device corresponds to the incident port 25, and the receiving end of the amplification spectral analysis device corresponds to the detection port 26. The amplification heating film 22 is attached to the surface of the amplification base 21 and is used to monitor the temperature of the amplification base 21, so as to regulate the heating temperature of the amplification heating film 22 so that the temperature inside the amplification tube 19 is at a preset amplification temperature. Preferably, a second sensor mounting hole is provided on the amplification base 21, and the amplification temperature sensor 23 is installed in the second sensor mounting hole. Usually, the amplification base 21 is made of metal or alloy, such as aluminum alloy, with good thermal conductivity, which can be approximately equal to the temperature of the reagent in the amplification tube 19. The receiving tank 9 is located in the light-shielding shell and is installed at the notch of the receiving tank. The notch of the receiving tank 9 is connected to the notch of the receiving tank, and the bottom of the receiving tank 9 is provided with an embedding port 35. The lower half of the amplification tube 19 is inserted into the insertion port 24, and the amplification base 21 is used to prevent light other than the LED light source from affecting the lower half of the amplification tube 19. After the closing cover 7 is covered, the receiving tank 9 and the closing cover 7 can prevent external light sources from affecting the upper half of the amplification tube 19. The amplification base 21, the receiving tank 9 and the closing cover 7 together form a darkroom environment, which can prevent light other than the LED light source from affecting the amplification tube 19 and reducing the monitoring accuracy.

[0036] In one embodiment, the amplification spectrum analysis device includes an LED light group and a spectrum sensor 32. The spectrum sensor 32 corresponds to the detection port 26, and the spectrum sensor 32 is located below the detection port 26. The LED light group includes a blue LED light 28, a yellow LED light 29, and a red LED light 30. Three incident ports 25 are provided on the amplification base 21. The three incident ports 25 are respectively arranged at three positions of the amplification tube 19, and the three incident ports 25 are all installed with filters 31. The three incident ports 25 correspond to the blue LED light 28, the yellow LED light 29, and the red LED light 30. The three filters 31 use filtering parameters with different nm values. Preferably, the two incident ports 25 located in opposite directions correspond to the blue LED light 28 and the red LED light 30, respectively, and the remaining incident port 25 corresponds to the yellow LED light 29. The filter 31 corresponding to the blue LED light 28 uses a 450nm~490nm filter, the filter 31 corresponding to the yellow LED light 29 uses a 560nm~600nm filter, and the filter 31 corresponding to the red LED light 30 uses a 648nm~688nm filter. Depending on the required spectral color, the blue LED light 28, the yellow LED light 29, or the red LED light 30 can be turned on, and then the spectral information is received by the spectral sensor 32. Compared with the LED light source + photodiode receiving detection method of the PCR amplification instrument, the LED light source + spectral sensor 32 receiving detection method has stronger anti-interference ability and does not require purification using the magnetic bead method. The sample in the lysate can be directly analyzed and detected.

[0037] In one embodiment, the LED lamp group is installed on a three-color lamp holder 18, and three lamp holes are provided on the three-color lamp holder 18. The blue LED lamp 28, the yellow LED lamp 29 or the red LED lamp 30 are respectively embedded in the three lamp holes. The three lamp holes correspond to three injection ports respectively. When wrapped by the three lamp holes, the leakage of the light source of the LED lamp can be reduced, and the light source can be injected into the injection port as much as possible.

[0038] In one embodiment, the closing cover 7 is hinged to the notch of the receiving groove 9 . The notch of the receiving groove 9 has a wall thickness capable of supporting the closing cover 7 . The closing cover 7 can be opened and closed by rotation.

[0039] In one embodiment, the closure cover 7 and the receiving slot 9 are connected by a spring-loaded latch comprising a locking tongue 10 and a locking tongue seat 11. One end of the closure cover 7 is connected to one end of the notch of the receiving slot 9. The other end of the closure cover 7 is provided with the locking tongue 10, and the other end of the notch of the receiving slot 9 is provided with the locking tongue seat 11, which receives the locking tongue 10. Locking is achieved by rotating the closure cover 7 and inserting the locking tongue 10 into the locking tongue seat 11. Pressing the closure cover 7 downward causes the locking tongue 10 to automatically pop out of the locking tongue seat 11, unlocking the door. This spring-loaded latch is conventional technology and will not be described in detail here.

[0040] In one embodiment, the closure cap 7 is further provided with a silicone pad 8. When the locking tongue 10 and the locking tongue seat 11 are locked, the silicone pad 8 presses against the sealing plug 20 of the amplification tube 19, pressing the amplification tube 19 into the insertion opening 24 of the amplification base 21. The insertion opening 24 has a conical structure, and the lower half of the amplification tube 19 also has a conical shape, which facilitates the insertion and positioning of the lower half of the amplification tube 19 and the insertion opening 24.

[0041] In one embodiment, the amplification spectrum analysis device further includes a constant-temperature amplification spectrum circuit board 27. The amplification heating film 22, the amplification temperature sensor 23, the blue LED light 28, the yellow LED light 29, and the red LED light 30 are all electrically connected to the constant-temperature amplification spectrum circuit board 27. The constant-temperature amplification spectrum circuit board 27 uniformly controls the operation of the amplification heating film 22, the amplification temperature sensor 23, the blue LED light 28, the yellow LED light 29, and the red LED light 30.

[0042] In one embodiment, a display screen 4 is provided on the light-shielding housing, and a control integrated circuit board 12 is housed within the light-shielding housing. The heating device 16, the display screen 4, and the constant-temperature amplification spectroscopy circuit board 27 are all electrically connected to the control integrated circuit board 12. The display screen 4 can output control information, which, through the control integrated circuit board 12, can send control instructions to the heating device 16 and the constant-temperature amplification spectroscopy circuit board 27 for control. The display screen 4 can also display temperature information monitored by the pyrolysis temperature sensor, temperature information monitored by the amplification temperature sensor 23, analysis results from the spectrum sensor 32, and process information from the LED light assembly, thereby visualizing operations and information, facilitating operation and information acquisition by test personnel. Preferably, the display screen 4 and the control integrated circuit board 12 are electrically connected via a cable.

[0043] In one embodiment, the display screen 4 is an AMOLED screen, and the size is set as needed, such as 2.06 inches. Of course, the display screen 4 can also be other screens.

[0044] In one embodiment, a power supply interface 6 is provided on the light shielding shell, and power is supplied to the electrical components in the light shielding shell through the power supply interface 6 .

[0045] In one embodiment, the power supply interface 6 is a USB-C interface or a Type-C interface, which is electrically connected to the control integrated circuit board 12. The USB-C interface or the Type-C interface is used to power the device and can also be used as a debugging and upgrade interface to upgrade the control software in the control integrated circuit board 12.

[0046] In one embodiment, the control integrated circuit board 12 is provided with a WIFI module and a Bluetooth module, which can transmit information to a background monitoring device (such as a mobile phone) or upload it to the network.

[0047] In one embodiment, the light shielding housing is provided with heat dissipation holes 5, and a cooling fan 17 is disposed within the housing. The cooling fan 17 is electrically connected to the control integrated circuit board 12. The cooling fan 17 drives air flow within the light shielding housing, which is discharged through the heat dissipation holes 5, thereby dissipating heat within the light shielding housing and avoiding the problem of excessive heat generation caused by the enclosed space. Preferably, the light shielding housing is a rectangular housing, with heat dissipation holes 5 disposed on the left, right, and rear sides of the rectangular housing. The cooling fan 17 corresponds to the heat dissipation holes 5 on the rear side. The front side of the rectangular housing is provided with a mounting opening 36 for mounting the display screen 4.

[0048] In one embodiment, the light-shielding shell includes an upper light-shielding shell 1 and a lower light-shielding shell 2, the upper light-shielding shell 1 is detachably connected to the lower light-shielding shell 2, and the heat dissipation holes 5, the display screen 4, the extension port 34 and the receiving slot 9 are arranged on the upper light-shielding shell 1. The heating device 16 in the lysis module is installed on the lower light-shielding shell 2 through the mounting bracket 15. The constant temperature amplification spectrum circuit board 17 in the amplification analysis module is installed on the lower light-shielding shell 2 through the three-color lamp holder 18. The control integrated circuit board 12, the cooling fan 17 and the power supply interface 6 are all installed on the lower light-shielding shell 2. The upper light-shielding shell 1 and the lower light-shielding shell 2 are detachably connected, which is conducive to the maintenance and replacement of some components in the lysis module and the amplification analysis module.

[0049] In one embodiment, the upper light shielding shell 1 and the lower light shielding shell 2 are connected by screws. Screw holes 33 are correspondingly provided on the upper light shielding shell 1 and the lower light shielding shell 2 for screwing in screws to achieve detachable connection.

[0050] In one embodiment, a non-slip rubber pad 3 is provided on the bottom of the light-shielding housing lower shell 2. Preferably, the non-slip rubber pad 3 is made of silicone.

[0051] The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The above examples are only intended to help understand the method and core concept of the present invention. At the same time, those skilled in the art will find that the specific implementation methods and application scopes may vary based on the concept of the present invention. In summary, the contents of this specification should not be construed as limiting the present invention.

Claims

1. A nucleic acid detector integrating lysis and amplification, characterized in that: It includes a light-shielding shell, in which a lysis module and an amplification analysis module are installed. The lysis module includes a lysis tube and a heating device. The heating device is provided with a heating port for plugging and unplugging the lysis tube. The amplification analysis module includes an amplification tube, a constant temperature device for providing a constant temperature environment for the amplification tube, and a spectral analysis device for performing spectral analysis on the sample in the amplification tube. The top of the light-shielding shell is provided with a protruding port and a receiving groove. The protruding port is coaxially arranged above the heating port, and the tube mouth of the lysis tube is extended through the protruding port. The groove of the receiving groove is provided with a closing cover, and the bottom of the receiving groove is provided with an embedding port. The embedding port allows the upper half of the amplification tube to extend into the receiving groove. The constant temperature device is provided with an insertion port for the lower half of the amplification tube to be plugged and unplugged.

2. The integrated nucleic acid detector for lysis and amplification according to claim 1, characterized in that: The tube housing of the lysis tube is provided with a tube cap, and the tube opening of the amplification tube is provided with a sealing plug.

3. The integrated nucleic acid detector for lysis and amplification according to claim 1, characterized in that: The heating device includes a heating seat, a cracking heating film and a cracking temperature sensor. The heating port is located on the heating seat, the cracking heating film is arranged on the inner wall of the heating port, and the cracking temperature sensor is arranged in the heating seat. The cracking temperature sensor is used to monitor the temperature of the cracking heating film.

4. The integrated nucleic acid detector for lysis and amplification according to claim 3, characterized in that: The constant temperature device includes an amplification base, an amplification heating film and an amplification temperature sensor. The insertion port is arranged on the top wall of the amplification base. The side wall of the amplification base is provided with an incident port connected to the insertion port. The bottom wall of the amplification base is provided with a detection port connected to the insertion port. The incident end and the receiving end of the amplification spectrum analysis device correspond to the incident port and the detection port respectively. The amplification heating film is attached to the surface of the amplification base. The amplification temperature sensor is used to monitor the temperature of the amplification heating film.

5. The integrated nucleic acid detector for lysis and amplification according to claim 4, characterized in that: The amplification spectrum analysis device includes an LED light group and a spectrum sensor. The spectrum sensor corresponds to the detection port and is located below the detection port. The LED light group includes a blue LED light, a yellow LED light and a red LED light. Three incident ports are provided on the amplification base. The three incident ports are located at three positions of the amplification tube. Filters are installed on the three incident ports, and the three incident ports correspond to the blue LED light, the yellow LED light and the red LED light respectively.

6. The integrated nucleic acid detector for lysis and amplification according to claim 4, characterized in that: The closing cover is hinged on the notch of the receiving groove, and the notch of the receiving groove has a wall thickness capable of supporting the closing cover.

7. The integrated nucleic acid detector for lysis and amplification according to claim 6, characterized in that: The closing cover and the accommodating slot are connected by a spring-pressed latch, which includes a lock tongue and a lock tongue seat. One end of the closing cover is hinged to one end of the notch of the accommodating slot, the lock tongue is arranged at the other end of the closing cover, and the lock tongue seat is arranged on the other end of the notch of the accommodating slot.

8. The integrated nucleic acid detector for lysis and amplification according to claim 5, characterized in that: The amplification spectrum analysis device also includes a constant temperature amplification spectrum circuit board, and the amplification heating film, the amplification temperature sensor, the blue LED light, the yellow LED light and the red LED light are all electrically connected to the constant temperature amplification spectrum circuit board.

9. The integrated nucleic acid detector for lysis and amplification according to claim 8, characterized in that: A display screen is provided on the light-shielding shell, a control integrated circuit board is provided in the light-shielding shell, the heating device, the display screen and the constant temperature amplification spectrum circuit board are all electrically connected to the control integrated circuit board, and the display screen can display the detection results.

10. The integrated nucleic acid detector for lysis and amplification according to claim 9, characterized in that: A power supply interface is provided on the light-shielding shell.

11. The integrated nucleic acid detector for lysis and amplification according to claim 10, characterized in that: The power supply interface is a USB-C port.

12. The integrated nucleic acid detector for lysis and amplification according to claim 10, characterized in that: The light shielding shell is provided with heat dissipation holes, a heat dissipation fan is provided in the light shielding shell, and the heat dissipation fan is electrically connected to the control integrated circuit board.

13. The integrated nucleic acid detector for lysis and amplification according to claim 12, characterized in that: The light-shielding shell includes a lower light-shielding shell and an upper light-shielding shell. The upper light-shielding shell is detachably connected to the lower light-shielding shell. The heat dissipation holes, the power supply interface, the display screen, the extension port and the notch of the accommodating slot are all arranged on the upper light-shielding shell. The lysis module and the amplification analysis module are both installed on the lower light-shielding shell.

14. The integrated nucleic acid detector for lysis and amplification according to claim 13, characterized in that: An anti-slip rubber pad is provided on the bottom of the lower shell of the light-shielding shell.

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