A skin care product with long-lasting moisturizing and anti-wrinkle effects and a preparation process thereof

Abstract

The present application relates to a kind of long-term moisturizing and anti-wrinkle effect skin care product and its preparation process, belong to skin care product technical field.The long-term moisturizing and anti-wrinkle effect skin care product, by mass percentage calculation includes 1-5% active substance composition and 95-99% cosmetic base, the active substance composition includes the following raw materials by mass fraction: ginkgo biloba extract 6-12 parts, black Ganoderma lucidum fermentation 10-15 parts, phyllanthus emblica extract 8-12 parts, Selaginella extract 15-18 parts, chlorella exosome 5-8 parts, desert rose callus fermentation 15-20 parts.The present application is compounded by black Ganoderma lucidum fermentation, phyllanthus emblica extract, ginkgo biloba extract, Selaginella extract, chlorella exosome, desert rose callus fermentation, so that the skin care product of the present application has the effect of inhibiting the generation of wrinkle, long-term moisturizing, antioxidant and the like.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of skin care products, and particularly relates to a skin care product with long-lasting moisturizing and anti-wrinkle effects and a preparation process thereof. BACKGROUND

[0002] Skin, as the outermost organ of the human body, plays a very important role in protecting and maintaining the normal circulation and metabolism of the human body and the balance of water in the body. Normal skin is soft, smooth and elastic, and has a certain protective effect on the body. However, when the elastic fibers of the skin decrease and the water content decreases, the skin becomes rough and wrinkles appear, and the skin begins to age. With the increase of physiological age, under the combined action of the inherent physiological mechanism of human aging, UV radiation and environmental pollution, the skin gradually ages, and skin aging is usually manifested in the decrease of skin elasticity and the generation of wrinkles. In daily life, the use of moisturizing skin care products alone is not enough to prevent skin aging. Factors such as environment, UV and stress will increase free radicals, which will greatly harm the skin and accelerate skin aging. Therefore, it is necessary to use some skin care products with anti-aging effect to reduce the generation of wrinkles and delay the speed of aging. At present, the existing anti-wrinkle skin care products on the market are easy to irritate the skin and have limited effects. SUMMARY

[0003] The first object of the present application is to provide a skin care product with long-lasting moisturizing and anti-wrinkle effects, which has the effects of moisturizing, anti-wrinkling and anti-aging.

[0004] The second object of the present application is to provide a preparation process of a skin care product with long-lasting moisturizing and anti-wrinkle effects.

[0005] In order to achieve the above objects, the technical scheme adopted by the present application is as follows:

[0006] A skin care product with long-lasting moisturizing and anti-wrinkle effects, comprising 1-5% of an active substance composition and 95-99% of a cosmetic base by mass percentage.

[0007] The active substance composition comprises the following raw materials by mass fraction: Ginkgo biloba extract 6-12 parts, black Ganoderma lucidum fermentation 10-15 parts, Phyllanthus emblica extract 8-12 parts, Selaginella uncinata extract 15-18 parts, Chlorella vulgaris exosome 5-8 parts, and desert rose callus fermentation 15-20 parts.

[0008] Further, the preparation method of the desert rose callus ferment is as follows: the desert rose callus is crushed, 5-10 times of ethanol is added for heating extraction for 2-3 times, filtration, the ethanol is recovered after the filtrate is concentrated, sterilization is performed to obtain a fermentation substrate, the fermentation substrate is mixed with sterile water containing sucrose to obtain a desert rose callus mixed solution, the fermentation is performed by inoculating the fermentation bacteria in the desert rose callus mixed solution at a volume ratio of 1-5%, and then filtration and inactivation are performed to obtain the desert rose callus ferment.

[0009] Further, the fermentation bacteria are mixed bacteria of lactobacillus plantarum and bacillus megaterium, the weight ratio of the lactobacillus plantarum to the bacillus megaterium is 1:2-3, the viable bacterial count of the lactobacillus plantarum in the fermentation bacteria is 1.8-2×10 7 CFU / g, and the viable bacterial count of the bacillus megaterium in the fermentation bacteria is 1-1.5×10 7 CFU / g.

[0010] Further, the fermentation condition of inoculating the fermentation bacteria for fermentation is 25-30℃ for 5-7 days.

[0011] Further, the heating temperature of the heating extraction is 50-55℃, and the extraction time is 1.5-3h; the mass ratio of the fermentation substrate to the sterile water containing sucrose is 1:20-25.

[0012] Further, the preparation method of the black ganoderma ferment is as follows: the black ganoderma is crushed and sieved to obtain black ganoderma powder, 10-15 times of ethanol is added, and extraction is performed at 40-50℃ for 1-2h, filtration is performed, the ethanol is removed after the filtrate is concentrated to obtain a black ganoderma extract, the fermentation bacteria are inoculated in the black ganoderma extract at a volume ratio of 1-5%, fermentation is performed at 30-35℃ for 5-7 days, and then sterilization is performed, and the black ganoderma ferment is obtained after cooling; the fermentation bacteria are mixed bacteria of lactobacillus rhamnosus and bacillus amyloliquefaciens, and the mass ratio of the lactobacillus rhamnosus to the bacillus amyloliquefaciens is 1:1-5.

[0013] Further, the viable bacterial count of the lactobacillus rhamnosus in the fermentation bacteria is 2-4×10 7 CFU / g, and the viable bacterial count of the bacillus amyloliquefaciens in the fermentation bacteria is 1.5-3×10 7 CFU / g.

[0014] Further, the cosmetic base comprises 5-10% of a moisturizing agent, 1-5% of an emulsifying agent, 0.4-0.8% of a preservative, 0.1-0.3% of a thickening agent, 0.05-0.15% of a fragrance, and the balance of water.

[0015] Further, the emulsifier is one or more of cetylstearyl alcohol, cetylstearyl glucoside, glycerin stearate; the humectant is one or more of glycerin, butylene glycol, panthenol, sodium hyaluronate, allantoin; the preservative is one or more of phenoxyethanol, sorbic acid, potassium sorbate, p-hydroxyacetophenone; the thickening agent is one or more of xanthan gum, carbomer, cellulose gum, betaine.

[0016] The preparation process of the skin care product with long-acting moisturizing and anti-wrinkle effects comprises the following steps: uniformly stirring water, a humectant and a thickening agent to obtain phase A, adding an emulsifier into phase A for emulsification and homogenization, reducing the temperature to room temperature, then adding an active substance composition, a preservative and a fragrance, and stirring at 40-50 DEG C for 5-30 min to obtain the product.

[0017] The beneficial effects of the present application are:

[0018] The black ganoderma is rich in triterpenoids, polysaccharides, polyphenols and amino acids, and has the effects of antioxidant, anti-aging, anti-wrinkle, etc.; the emblic leafflower is rich in flavonoids and polyphenols, and has the effects of moisturizing, antioxidant, etc.; the ginkgo leaf is rich in flavonoids and terpenoids, and has the effects of free radical scavenging, fine line lightening and anti-inflammatory, etc.; the selaginella is long-acting moisturizing and anti-inflammatory. The present application makes the active ingredients in the desert rose callus and the black ganoderma more rich in types and higher in concentration through fermentation of the desert rose callus and the black ganoderma, and improves the anti-wrinkle performance and the moisturizing performance of the desert rose callus and the black ganoderma. The skin care product of the present application has the effects of inhibiting wrinkle generation, long-acting moisturizing, antioxidant, etc. through compounding of the black ganoderma fermentation product, the emblic leafflower extract, the ginkgo leaf extract, the selaginella extract, the chlorella exosome and the desert rose callus fermentation product. DETAILED DESCRIPTION

[0019] The present application will be further described below in combination with the embodiments.

[0020] The lactobacillus rhamnosus has the preservation number of CGMCC 1.60148.

[0021] The bacillus amyloliquefaciens has the preservation number of CCTCC NO: M 20191088.

[0022] The lactobacillus plantarum has the preservation number of CGMCC. NO. 11172.

[0023] The bacillus megaterium has the preservation number of GDMCC No: 60467.

[0024] The lactobacillus rhamnosus, the bacillus amyloliquefaciens, the lactobacillus plantarum and the bacillus megaterium used in the present application have been disclosed before the filing date.

[0025] Example 1

[0026] The skin care product for long-lasting moisturizing and anti-wrinkle efficacy of Example 1 comprises the following raw materials: 5 kg of active ingredient composition and 95 kg of cosmetic base.

[0027] The active ingredient composition comprises 10 kg of Ginkgo biloba leaf extract, 10 kg of black Ganoderma lucidum fermentation, 8 kg of Phyllanthus emblica extract, 15 kg of Selaginella moellendorffii extract, 5 kg of Chlorella vulgaris exosome, and 15 kg of Adenium obesum callus fermentation.

[0028] The preparation method of the Adenium obesum callus fermentation is as follows: the Adenium obesum callus is mechanically pulverized under a pressure of 200 bar, and then 5 times the amount of the total amount of the pulverized callus, 75% ethanol solution by volume is added, and extraction is performed at 50°C for 2 hours, twice. The filtrate is concentrated and ethanol is recovered, and the fermentation substrate is obtained by sterilization at 110°C for 10 minutes. The fermentation substrate is mixed with sterile water containing 5wt% sucrose at a mass ratio of 1:20 to obtain an Adenium obesum callus mixture. The fermentation bacteria are inoculated in the Adenium obesum callus mixture at a volume ratio of 2%, and fermentation is performed at 25°C for 7 days. Then, the fermentation bacteria are filtered and inactivated using a filter membrane with a pore size of 0.22μm to obtain the Adenium obesum callus fermentation. The weight ratio of Lactobacillus plantarum to Bacillus megaterium in the fermentation bacteria is 1:2, the viable bacterial count of Lactobacillus plantarum is 2×10 7 CFU / g, and the viable bacterial count of Bacillus megaterium is 1×10 7 CFU / g.

[0029] The preparation method of the black Ganoderma lucidum fermentation is as follows: black Ganoderma lucidum powder is obtained by crushing and sieving black Ganoderma lucidum, 15 times the amount of 75% ethanol by volume is added, and extraction is performed at 40°C for 2 hours. The filtrate is concentrated and ethanol is removed to obtain a black Ganoderma lucidum extract. The fermentation bacteria are inoculated in the black Ganoderma lucidum extract at a volume ratio of 2%, and fermentation is performed at 30°C for 5 days. Then, the fermentation bacteria are sterilized at 120°C for 5 minutes, and the black Ganoderma lucidum fermentation is obtained after cooling. The mass ratio of Lactobacillus rhamnosus to Bacillus amyloliquefaciens in the fermentation bacteria is 1:1, the viable bacterial count of Lactobacillus rhamnosus is 2×10 7 CFU / g, and the viable bacterial count of Bacillus amyloliquefaciens is 2×10 7 CFU / g.

[0030] The cosmetic base comprises 1 kg of cetylstearyl alcohol, 2 kg of cetearyl glucoside, 3 kg of glycerol, 5 kg of panthenol, 2 kg of allantoin, 0.1 kg of phenoxyethanol, 0.3 kg of potassium sorbate, 0.1 kg of xanthan gum, 0.05 kg of betaine, and 0.1 kg of fragrance, and water is supplemented to 100 kg.

[0031] The preparation process of the skin care product with long-lasting moisturizing and anti-wrinkle efficacy of Example 1 is as follows: uniformly stir water, moisturizing agent and thickening agent to obtain phase A, emulsify and homogenize the emulsifier in phase A, cool to room temperature, then add active ingredient composition, preservative and fragrance, stir at 40°C for 20 min, and obtain.

[0032] Example 2

[0033] The skin care product with long-lasting moisturizing and anti-wrinkle efficacy of Example 2 comprises the following raw materials: 3 kg of active ingredient composition and 97 kg of cosmetic base.

[0034] The active ingredient composition comprises 6 kg of Ginkgo biloba leaf extract, 15 kg of black Ganoderma lucidum fermentation, 10 kg of Phyllanthus emblica extract, 16 kg of Selaginella moellendorffii extract, 6 kg of Chlorella vulgaris exosome, and 20 kg of Adenium obesum callus fermentation.

[0035] The preparation method of the Adenium obesum callus fermentation is as follows: mechanically pulverize the Adenium obesum callus under a pressure of 200 bar, then add 10 times the amount of the crushed callus, 75% ethanol solution by volume fraction, extract at 55°C for 1.5 h, extract for 3 times, filter, concentrate the filtrate to recover ethanol, sterilize at 110°C for 10 min to obtain fermentation substrate, mix the fermentation substrate with sterile water containing 5 wt% sucrose at a mass ratio of 1:25 to obtain Adenium obesum callus mixed solution, inoculate the fermentation bacteria in the Adenium obesum callus mixed solution at a volume ratio of 1%, ferment at 28°C for 5 days, then filter and inactivate using a filter membrane with a pore size of 0.22 μm to obtain the Adenium obesum callus fermentation. The weight ratio of Lactobacillus plantarum to Bacillus megaterium in the fermentation bacteria is 1:3, the viable bacterial count of Lactobacillus plantarum is 1.8×10 7 CFU / g, and the viable bacterial count of Bacillus megaterium is 1×10 7 CFU / g.

[0036] The preparation method of the black Ganoderma lucidum fermentation is as follows: pulverize and sieve the black Ganoderma lucidum to obtain black Ganoderma lucidum powder, add 10 times the amount of 75% ethanol by volume fraction, extract at 50°C for 1 h, filter, concentrate the filtrate to remove ethanol to obtain black Ganoderma lucidum extract, inoculate the fermentation bacteria in the black Ganoderma lucidum extract at a volume ratio of 3%, ferment at 35°C for 7 days, then sterilize at 120°C for 8 min, and obtain the black Ganoderma lucidum fermentation after cooling. The mass ratio of Lactobacillus rhamnosus to Bacillus amyloliquefaciens in the fermentation bacteria is 1:4, the viable bacterial count of Lactobacillus rhamnosus is 3×10 7 CFU / g, and the viable bacterial count of Bacillus amyloliquefaciens is 1.5×10 7 CFU / g.

[0037] The cosmetic base comprises 1 kg of cetyl stearyl alcohol, 2 kg of cetyl stearyl glucoside, 2 kg of glyceryl stearate, 3 kg of glycerol, 2 kg of butylene glycol, 3 kg of sodium hyaluronate, 0.1 kg of phenoxyethanol, 0.3 kg of potassium sorbate, 0.2 kg of p-hydroxyacetophenone, 0.1 kg of xanthan gum, 0.05 kg of betaine, 0.1 kg of carbomer, and 0.15 kg of fragrance, and water is supplemented to 100 kg.

[0038] The preparation process of the skin care product with long-lasting moisturizing and anti-wrinkle efficacy of Example 2 is as follows: uniformly stirring water, moisturizing agent, and thickening agent to obtain phase A, adding emulsifier to phase A for emulsification and homogenization, cooling to room temperature, then adding active ingredient composition, preservative, and fragrance, stirring at 50°C for 10 min, and obtaining the product.

[0039] Example 3

[0040] The skin care product with long-lasting moisturizing and anti-wrinkle efficacy of Example 3 comprises the following raw materials: 1 kg of active ingredient composition and 99 kg of cosmetic base.

[0041] The active ingredient composition comprises 12 kg of Ginkgo biloba leaf extract, 12 kg of black Ganoderma lucidum fermentation product, 12 kg of Phyllanthus emblica extract, 18 kg of Selaginella moellendorffii extract, 8 kg of Chlorella vulgaris exosome, and 16 kg of Adenium obesum callus fermentation product.

[0042] The preparation method of the Adenium obesum callus fermentation product is as follows: mechanically pulverizing Adenium obesum callus under a pressure of 200 bar, then adding 8 times the amount of the pulverized callus and 75% ethanol solution by volume to extract at 50°C for 3 h, extracting twice, filtering, recovering ethanol after concentrating the filtrate, sterilizing at 110°C for 10 min to obtain fermentation substrate, mixing the fermentation substrate with sterile water containing 5 wt% sucrose at a mass ratio of 1:20 to obtain Adenium obesum callus mixed solution, inoculating fermentation bacteria in the Adenium obesum callus mixed solution at a volume ratio of 5%, and fermenting at 30°C for 5 d, then filtering and inactivating using a filter membrane with a pore size of 0.22 μm to obtain the Adenium obesum callus fermentation product. The weight ratio of Lactobacillus plantarum to Bacillus megaterium in the fermentation bacteria is 1:2, the viable bacterial count of Lactobacillus plantarum is 2×10 7 CFU / g, and the viable bacterial count of Bacillus megaterium is 1.5×10 7 CFU / g.

[0043] The preparation method of the Ganoderma atrum ferment is as follows: the Ganoderma atrum is crushed and sieved to obtain Ganoderma atrum powder, 10 times the amount of 75% volume fraction ethanol is added, and extraction is carried out at 45°C for 2 hours, and then filtration is performed; the filtrate is concentrated and the ethanol is removed to obtain a Ganoderma atrum extract; 5% by volume of fermentation bacteria is inoculated into the Ganoderma atrum extract, and fermentation is carried out at 30°C for 5 days, followed by sterilization at 120°C for 5 minutes, and then the Ganoderma atrum ferment is obtained after cooling. The mass ratio of Lactobacillus rhamnosus and Bacillus amyloliquefaciens in the fermentation bacteria is 1:5, the viable count of Lactobacillus rhamnosus is 2×10 7 CFU / g, and the viable count of Bacillus amyloliquefaciens is 3×10 7 CFU / g.

[0044] The cosmetic base comprises 1 kg of cetylstearyl alcohol, 1 kg of cetylstearyl glucoside, 3 kg of allantoin, 5 kg of sodium hyaluronate, 0.1 kg of sorbic acid, 0.3 kg of potassium sorbate, 0.1 kg of cellulose gum, 0.05 kg of carbomer, 0.05 kg of betaine, and 0.1 kg of fragrance, and water is supplemented to 100 kg.

[0045] The preparation process of the skin care product with long-term moisturizing and anti-wrinkle effects of Example 3 is as follows: water, a moisturizing agent, and a thickening agent are stirred uniformly to obtain phase A, an emulsifier is added to phase A for emulsification and homogenization, and then the temperature is lowered to room temperature; subsequently, the active substance composition, a preservative, and a fragrance are added, and stirring is performed at 45°C for 30 minutes, and then the skin care product is obtained.

[0046] Comparative Example 1

[0047] The skin care product with long-term moisturizing and anti-wrinkle effects of Comparative Example 1 is substantially the same as that of Example 1, and the difference between the skin care product of Comparative Example 1 and that of Example 1 is that the Phyllanthus emblica extract is not added in Comparative Example 1, and the proportions of the Ginkgo biloba extract, the Ganoderma atrum ferment, the Selaginella moellendorffii extract, the Chlorella vulgaris exosome, and the Ochrocoris maritimus callus ferment in Example 1 are supplemented.

[0048] Comparative Example 2

[0049] The skin care product with long-term moisturizing and anti-wrinkle effects of Comparative Example 2 is substantially the same as that of Example 1, and the difference between the skin care product of Comparative Example 2 and that of Example 1 is that the Ganoderma atrum extract is not added in Comparative Example 2, and the proportions of the Ginkgo biloba extract, the Phyllanthus emblica extract, the Selaginella moellendorffii extract, the Chlorella vulgaris exosome, and the Ochrocoris maritimus callus ferment in Example 1 are supplemented.

[0050] Comparative Example 3

[0051] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 3 is substantially the same as that of Example 1, except that no Ginkgo biloba leaf extract is added in Comparative Example 3, and the proportions of the Ganoderma atrum extract, Phyllanthus emblica extract, Selaginella extract, Chlorella exosome, and desert rose callus ferment are supplemented according to the proportions in Example 1.

[0052] Comparative Example 4

[0053] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 4 is substantially the same as that of Example 1, except that no desert rose callus ferment is added in Comparative Example 4, and the proportions of the Ganoderma atrum extract, Phyllanthus emblica extract, Selaginella extract, Chlorella exosome, and Ginkgo biloba leaf extract are supplemented according to the proportions in Example 1.

[0054] Comparative Example 5

[0055] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 5 is substantially the same as that of Example 1, except that no Selaginella extract is added in Comparative Example 5, and the proportions of the Ganoderma atrum extract, Phyllanthus emblica extract, desert rose callus ferment, Chlorella exosome, and Ginkgo biloba leaf extract are supplemented according to the proportions in Example 1.

[0056] Comparative Example 6

[0057] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 6 is substantially the same as that of Example 1, except that no Chlorella exosome is added in Comparative Example 6, and the proportions of the Ganoderma atrum extract, Phyllanthus emblica extract, desert rose callus ferment, Selaginella extract, and Ginkgo biloba leaf extract are supplemented according to the proportions in Example 1.

[0058] Comparative Example 7

[0059] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 7 is substantially the same as that of Example 1, except that an equal amount of fermentation substrate is used instead of desert rose callus ferment in Comparative Example 7.

[0060] Comparative Example 8

[0061] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 8 is substantially the same as that of Example 1, except that an equal amount of Ganoderma atrum extract is used instead of Ganoderma atrum ferment in Comparative Example 8. The Ganoderma atrum extract is obtained by drying Ganoderma atrum extract liquid.

[0062] Comparative Example 9

[0063] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 9 is substantially the same as that of Example 1, except that the desert rose callus ferment of Comparative Example 9 is fermented using Lactobacillus plantarum, and the viable cell count of the Lactobacillus plantarum is 3 x 10 7 CFU / g.

[0064] Comparative Example 10

[0065] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 10 is substantially the same as that of Example 1, except that the desert rose callus ferment of Comparative Example 10 is fermented using Bacillus megaterium, and the viable cell count of the Bacillus megaterium is 3 x 10 7 CFU / g.

[0066] Comparative Example 11

[0067] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 11 is substantially the same as that of Example 1, except that the mass ratio of Lactobacillus plantarum and Bacillus megaterium in Comparative Example 11 is 1:1.

[0068] Comparative Example 12

[0069] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 12 is substantially the same as that of Example 1, except that the mass ratio of Lactobacillus plantarum and Bacillus megaterium in Comparative Example 12 is 1:4.

[0070] Comparative Example 13

[0071] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 13 is substantially the same as that of Example 1, except that the black ganoderma ferment of Comparative Example 13 is fermented using Lactobacillus rhamnosus, and the viable cell count of the Lactobacillus rhamnosus is 4 x 10 7 CFU / g.

[0072] Comparative Example 14

[0073] The long-term moisturizing and anti-wrinkle efficacy skincare product of Comparative Example 14 is substantially the same as that of Example 1, except that the black ganoderma ferment of Comparative Example 14 is fermented using Bacillus amyloliquefaciens, and the viable cell count of the Bacillus amyloliquefaciens is 4 x 10 7 CFU / g.

[0074] Comparative Example 15

[0075] The long-term moisturizing and anti-wrinkle efficacy skin care product of Comparative Example 15 is substantially the same as that of Example 1, except that the mass ratio of Lactobacillus rhamnosus to Bacillus amyloliquefaciens in Comparative Example 15 is 1:0.5.

[0076] Comparative Example 16

[0077] The long-term moisturizing and anti-wrinkle efficacy skin care product of Comparative Example 16 is substantially the same as that of Example 1, except that the mass ratio of Lactobacillus rhamnosus to Bacillus amyloliquefaciens in Comparative Example 16 is 1:6.

[0078] Test Example 1

[0079] Skin patch test

[0080] Ninety-five healthy skin volunteers aged 18-50 years were selected and randomly divided into 19 groups, each group corresponding to Example 1-3 and Comparative Example 1-16, and each group having 5 people. Suitable patch test materials were selected, and about 0.020g-0.025g of the test substance was placed in the patch test device. A low-sensitization adhesive tape was applied to the back of the volunteer, and the test substance was removed after 2 hours. Skin reactions were observed at 0.5, 24, and 48 hours after removal, respectively, and the results were recorded according to the current effective technical specification for skin reaction grading standards. The blank control was used as the negative control.

[0081] Evaluation criteria: Grade 0: negative reaction; Grade 1: suspicious reaction, only weak erythema; Grade 2: weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly papules; Grade 3: strong positive reaction (vesicle reaction): erythema, infiltration, edema, and possibly papules, and the reaction may exceed the test area; Grade 4: extremely strong positive reaction (fusion vesicle reaction): obvious erythema, severe infiltration, edema, fusion vesicles, and the reaction exceeds the test area.

[0082] Test results: All volunteers had negative skin reactions.

[0083] Test Example 2

[0084] Moisturizing effect test

[0085] Fifty-seven healthy skin volunteers aged 25-40 years were selected, who were not long-term outdoor workers; during the test period, the volunteers did not apply any unrelated drugs or cosmetics, and did not participate in any skin-related beauty or repair projects.

[0086] 57 volunteers were divided into 19 groups, 3 persons in each group, and the volunteers used the cleansing cream to clean up, then wiped off the moisture, sat still for 20 min in the environment of 25℃, humidity 60%, then detected the skin moisture content A1, then each group applied the skin care products of Examples 1-3 and Comparative Examples 1-16, respectively, and then detected the skin moisture content after 4h, 8h and 24h of application, respectively, which were A2, A3, A4, respectively, and each group was measured three times to take the average value, and the results were shown in Table 1.

[0087] The change rate of skin water content = (moisture content after applying skin care product - moisture content before applying skin care product) / moisture content before applying skin care product x 100%

[0088] Table 1 Moisturizing effect test results

[0089]

[0090]

[0091] Test Example 3

[0092] Transdermal water loss test

[0093] The volunteers of Test Example 2 were selected, and the skin care products of Examples 1-3 and Comparative Examples 1-16 were applied once in the morning and evening, respectively, for 56 consecutive days, and the skin moisture loss content tester Moisture Meter EpiD (Delfin, Finland) was used to detect the transdermal water loss content before use, on the 28th day and the 56th day of use, respectively, after cleansing in the environment of 25℃, humidity 60%, sat still for 30 min, unit: g / m 2 ·h, and the average value was taken, and the results were shown in Table 2.

[0094] △TEWL = (28d transdermal water loss / 56d transdermal water loss - 0d transdermal water loss) / 0d transdermal water loss x 100%

[0095] Table 2 Transdermal water loss test results

[0096]

[0097]

[0098] Test Example 4

[0099] Anti-wrinkle effect test

[0100] Select the volunteers of test example 2, respectively in the morning, evening each smear once the skin care product of example 1-3, counter example 1-16, continuously use 56 days, before using, the 28th day and the 56th day of using, use Germany CK company MPA580 skin elasticity tester and Germany PROMISPICO wrinkle measuring instrument to determine skin elasticity (R) and wrinkle (SEW), take average value, R is closer to 1, indicate that skin elasticity is better.SEW is bigger, indicate that wrinkle is more and deeper, the result is shown in table 3.

[0101] Table 3 Anti-wrinkle test results

[0102]

[0103]

[0104] From table 1-3, it can be seen that the skin care product with long-acting moisturizing and anti-wrinkle effect of the present application has good instant moisturizing and long-acting moisturizing effect, and can effectively remove wrinkles. According to counter examples 1-6 and example 1, it can be seen that the black ganoderma extract, phyllanthus emblica extract, desert rose callus fermentation product, chlorella exosome, selaginella extract and ginkgo leaf extract have good synergistic effect. According to counter examples 7, 8 and example 1, it can be seen that the fermented black ganoderma and desert rose callus have better moisturizing and anti-wrinkle effect. According to counter examples 9-16, it can be seen that the ratio of fermentation bacteria has a significant influence on the performance of desert rose callus fermentation product and black ganoderma fermentation product.

Claims

1. A skincare product with long-lasting moisturizing and anti-wrinkle effects, characterized in that, It consists of 1-5% active ingredient composition and 95-99% cosmetic base by weight percentage; The active substance composition consists of 6-12 parts by weight of Ginkgo biloba extract, 10-15 parts of Ganoderma lucidum ferment, 8-12 parts of Phyllanthus emblica extract, 15-18 parts of Selaginella tamariscina extract, 5-8 parts of Chlorella exosomes, and 15-20 parts of Rosa desertica callus ferment. The preparation method of the desert rose callus ferment is as follows: Desert rose callus is pulverized, and then extracted 2-3 times with 5-10 times the amount of ethanol by heating. After filtration and concentration of the filtrate, the ethanol is recovered and sterilized to obtain the fermentation substrate. The fermentation substrate is mixed with sterile water containing sucrose to obtain a desert rose callus mixture. A mixed strain of *Lactobacillus plantarum* and *Bacillus megaterium* is inoculated into the desert rose callus mixture at a volume ratio of 1-5% for fermentation. The mixture is then filtered and inactivated to obtain the desert rose callus ferment. The weight ratio of *Lactobacillus plantarum* to *Bacillus megaterium* is 1:2-3; the viable count of *Lactobacillus plantarum* is 1.8-2 × 10⁻⁶. 7 CFU / g, viable count of Bacillus megaterium is 1–1.5 × 10⁻⁶. 7 CFU / g; The preparation method of the black Ganoderma lucidum ferment is as follows: black Ganoderma lucidum is pulverized and sieved to obtain black Ganoderma lucidum powder. 10-15 times the amount of ethanol is added, and extraction is carried out at 40-50℃ for 1-2 hours. After filtration, the filtrate is concentrated and the ethanol is removed to obtain black Ganoderma lucidum extract. A mixed culture of *Lactobacillus rhamnosus* and *Bacillus amyloliquefaciens* is inoculated into the black Ganoderma lucidum extract at a volume ratio of 1-5%. Fermentation is carried out at 30-35℃ for 5-7 days, followed by sterilization and cooling to obtain the black Ganoderma lucidum ferment. The mass ratio of *Lactobacillus rhamnosus* to *Bacillus amyloliquefaciens* is 1:1-5; the viable count of *Lactobacillus rhamnosus* is 2-4 × 10⁻⁶. 7 CFU / g, viable count of Bacillus amyloliquefaciens is 1.5–3 × 10⁻⁶. 7 CFU / g; Lactobacillus rhamnosus has the accession number CGMCC 1.60148; Bacillus amyloliquefaciens has the accession number CCTCC NO: M20191088; Lactobacillus plantarum has the accession number CGMCC.NO.11172; and Bacillus megaterium has the accession number GDMCC No: 60467.

2. The skincare product with long-lasting moisturizing and anti-wrinkle effects according to claim 1, characterized in that, In the method for preparing the desert rose callus ferment, the fermentation conditions for inoculating a mixed strain of Lactobacillus plantarum and Bacillus megaterium are: fermentation at 25-30°C for 5-7 days.

3. The skincare product with long-lasting moisturizing and anti-wrinkle effects according to claim 1, characterized in that, In the method for preparing the desert rose callus ferment, the heating temperature for the heat extraction is 50-55℃, and the extraction time is 1.5-3h; the mass ratio of the fermentation substrate to sterile water containing sucrose is 1:20-25.

4. The skincare product with long-lasting moisturizing and anti-wrinkle effects according to claim 1, characterized in that, The cosmetic base comprises, by weight percentage, 5-10% moisturizer, 1-5% emulsifier, 0.4-0.8% preservative, 0.1-0.3% thickener, 0.05-0.15% fragrance, and the balance being water.

5. The skincare product with long-lasting moisturizing and anti-wrinkle effects according to claim 4, characterized in that, The emulsifier is one or more of cetearyl alcohol, cetearyl glucoside, and glyceryl stearate; the humectant is one or more of glycerin, butylene glycol, panthenol, sodium hyaluronate, and allantoin; the preservative is one or more of phenoxyethanol, sorbic acid, potassium sorbate, and p-hydroxyacetophenone; and the thickener is one or more of xanthan gum, carbomer, cellulose gum, and betaine.

6. A preparation process for a skincare product with long-lasting moisturizing and anti-wrinkle effects as described in claim 4, characterized in that, The process includes the following steps: mixing water, humectant, and thickener to obtain phase A; adding emulsifier to phase A and emulsifying and homogenizing; cooling to room temperature; then adding active ingredient composition, preservative, and fragrance; and stirring at 40–50°C for 5–30 minutes to obtain the final product.

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