Sex pheromone luring core for adult peppermint as well as preparation method and application of sex pheromone luring core

Abstract

The invention discloses a peppermint adult sex pheromone lure as well as a preparation method and application thereof, and a peppermint adult attractant in the lure comprises cis-7-tetradecenal, palmitic acid and linalyl acetate in a weight ratio of (10-15): (1-2): (1-2). According to the technical scheme, compared with the prior art, according to the sex pheromone luring core for the adult peppermint and the preparation method of the sex pheromone luring core for the adult peppermint, the attractant with a specific formula is utilized, the peppermint and the flatheaded borer can be specifically licked, and the prevention and control efficiency of the flatheaded borer is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and in particular relates to a sex pheromone attractant core for adult Zanthoxylum bungeanum borer, and a preparation method and application thereof. Background Art

[0002] The Sichuan pepper borer, Agrilus zanthoxylumi, belongs to the order Coleoptera, family Buprestidae, and genus Agrilus. It is a devastating pest of Sichuan pepper trees, often causing yield reductions and even the death of pepper trees in their prime. It is found in all major pepper-producing areas in northern my country, with frequent reports of infestations in Shaanxi and Gansu provinces. In Shaanxi, Lantian, Baoji, Weinan, Hancheng, and Fuping are particularly severely affected, while Tianshui, Wudu, and Wenxian are particularly affected in Gansu. Additionally, reports have been published in Henan, Sichuan, Shandong, and Shanxi provinces. In recent years, the area of ​​infestation by the Sichuan pepper borer has been expanding, and the severity of the damage has been increasing.

[0003] Current control measures for the Sichuan pepper borer still rely on traditional agricultural, physical, and chemical methods. The former consumes enormous amounts of manpower and resources. While the latter, while effective and capable of eliminating the Sichuan pepper borer to a certain extent and preventing its damage to the pepper plant, is prone to environmental pollution, leading to serious "three-residue" (R) problems. It also indiscriminately kills other beneficial insects, severely damaging insect populations and the insect food chain within the Sichuan pepper forest ecosystem. Furthermore, pesticide residues on Sichuan pepper leaves and fruits can affect its quality and hamper its import and export trade.

[0004] Chemical semiochemicals are not only specific for the Chinese pepper narrow borer but are also economical and environmentally friendly. Therefore, using these semiochemicals for forest pest control of Chinese pepper narrow borer aligns with the principles of green pest control and is currently the optimal approach. However, existing semiochemicals are still limited in their effectiveness against adult Chinese pepper narrow borer insects. Therefore, developing a pheromone lure for adult insects with high control efficiency and minimal environmental impact, as well as its preparation and application, is a pressing issue for those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a pheromone attractant core for controlling adult Zanthoxylum bungeanum, as well as a preparation method and application technology thereof. An attractant that specifically attracts adult Zanthoxylum bungeanum is added to the attractant core, thereby increasing the probability of trapping and killing adult Zanthoxylum bungeanum, reducing environmental pollution, and improving the control efficiency.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] One aspect of the present invention relates to a sex pheromone attractant for preventing and controlling adult Zanthoxylum bungeanum borer, characterized in that the Zanthoxylum bungeanum borer adult attractant in the attractant comprises: cis-7-tetradecenal, palmitic acid and linalyl acetate, and the weight ratio thereof is 10-15:1-2:1-2.

[0008] In a preferred embodiment of the present invention, the weight ratio of cis-7-tetradecenal, palmitic acid and linalyl acetate is 10-12:1-1.2:1-1.2.

[0009] In a preferred embodiment of the present invention, the solvent of the Zanthoxylum bungeanum adult attractant in the lure core is paraffin oil.

[0010] In a preferred embodiment of the present invention, the carrier of the bait core is agar.

[0011] Another aspect of the present invention relates to a method for preparing a sex pheromone attractant for controlling adult Zanthoxylum bungeanum, comprising the following steps: weighing agar powder, adding distilled water, and heating to completely dissolve the agar powder; while still hot, mixing the agar solution with an attractant for adult Zanthoxylum bungeanum, and naturally cooling and solidifying the solution to obtain an attractant.

[0012] Another aspect of the present invention also relates to the use of the above-mentioned sex pheromone attractant for controlling adult Zanthoxylum bungeanum in controlling Zanthoxylum bungeanum.

[0013] In a preferred embodiment of the present invention, the application includes the following steps: placing the prepared attractant core into a slow-release bottle, placing a slow-release bottle on each sticky insect board, and hanging the sticky insect board with the slow-release bottle on the pepper tree in the selected plot.

[0014] It can be seen from the above technical solution that compared with the existing technology, the present invention discloses a sex pheromone attractant core for adult Zanthoxylum bungeanum and a preparation method thereof. By using an attractant with a specific formula, the Zanthoxylum bungeanum can be specifically attracted, thereby improving the efficiency of bungeanum control. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 .Schematic diagram of the forest trapping device;

[0016] Figure 2 Daily attractant yield of Zanthoxylum bungeanum by attractant 1A, single component and control;

[0017] Figure 3 Daily attractant yield of Zanthoxylum bungeanum by attractant 2B, single component and control;

[0018] Figure 4 Daily attractant yield of Zanthoxylum bungeanum by attractant 3C, single component and control;

[0019] Figure 5Daily attractant yield of Zanthoxylum bungeanum by attractant 4D, single component and control;

[0020] Figure 6 . Daily attractant amount of Zanthoxylum bungeanum by attractant 5E, single component and control. DETAILED DESCRIPTION

[0021] To further understand the present invention, the following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0022] Unless otherwise specified, all reagents involved in the embodiments of the present invention are commercially available products and can be purchased through commercial channels.

[0023] Example 1

[0024] Since the environmental conditions in the forest are relatively complex and changeable, the attractant effect measured by the attractants screened indoors cannot fully explain their actual attractant effect in the forest. Therefore, the present invention conducts an attractant experiment in the forest to verify its actual attractant effect and provide an important theoretical basis for the large-scale application of attractants in the forest in the future.

[0025] Selection of experimental site: The experimental site was selected in the pepper field of Xilijiagou Village, Puhua Town, Lantian County, Xi'an City, Shaanxi Province.

[0026] Selection of components: Five attractants (formulas see Table 1) and their single ingredients were selected to make lures. Each group of experiments was treated with attractant, single ingredient 1, single ingredient 2, and single ingredient 3. The concentrations of the single ingredients were all optimal attractant concentrations, and the blank was used as the control.

[0027] Table 1 Final formulas of five attractants in forest trials

[0028]

[0029] Preparation of bait core: Weigh 3.0g agar powder, add 100mL distilled water, slowly heat and stir constantly to dissolve the agar powder completely; while hot, take 1mL agar solution and 0.5mL attractant or single component solution and add them to a 1.5mL centrifuge tube, cover the lid and shake the centrifuge tube to mix evenly, and be sure to open the lid to release steam; let the centrifuge tube stand, and it will become a bait core after solidification. Put the bait core into a self-sealing bag, mark it, and store it in the refrigerator at 4℃. Use within 5 days.

[0030] Hanging in the forest: put the prepared bait core into the slow-release bottle (the specification of the slow-release bottle is 10mm×10mm×20mm), put a slow-release bottle on each sticky insect board, and put the sticky insect board with the slow-release bottle (such as Figure 1 Lures (as shown) were hung from Zanthoxylum bungeanum trees in the selected plots at a uniform height of 2.0 m. Each treatment was placed from different directions on the same tree at the same height. Each treatment was replicated three times, with different treatments at least 10 m apart and randomly distributed throughout the Zanthoxylum bungeanum garden. Lures were replaced with new lures every morning, and the number of Zanthoxylum bungeanum beetles trapped on each sticky trap was recorded in the evening when the beetles were no longer flying. This was recorded for 10 consecutive days.

[0031] In order to further illustrate the feasibility of this embodiment, the results of the forest trapping experiment with the composite pheromone lure at different times are listed as follows: Figure 2-6 As shown:

[0032] The total number of Zanthoxylum serratum adults attracted by attractants 1A, diethylene glycol monostearate, cis-9-hexadecenal, α-phellandrene and control was as follows: attractant 1A (360) > cis-9-hexadecenal (237) > α-phellandrene (186) > diethylene glycol monostearate (163) > control (24).

[0033] The total number of Zanthoxylum serratum adults attracted by attractants 2B, pentadecanoic acid, cis-9-hexadecenal, α-phellandrene and control was as follows: attractant 2B (449) > cis-9-hexadecenal (240) > pentadecanoic acid (228) > α-phellandrene (196) > control (14).

[0034] The total number of Zanthoxylum serratum adults attracted by attractants 3C, pentadecanoic acid, oleic acid, d-limonene and control was as follows: attractant 3C (514) > oleic acid (226) > pentadecanoic acid (208) > d-limonene (194) > control (31).

[0035] The total number of Zanthoxylum serratum adults attracted by attractants 4D, cis-7-tetradecenal, cis-9-hexadecenal, d-limonene and the control was as follows: attractant 4D (507) > cis-9-hexadecenal (236) > cis-7-tetradecenal (235) > d-limonene (193) > control (23).

[0036] The total number of Zanthoxylum bungeanum adults attracted by attractants 5E, cis-7-tetradecenal, palmitic acid, linalyl acetate and control are as follows: attractant 5E (543) > cis-7-tetradecenal (235) > linalyl acetate (188) > palmitic acid (172) > control (29).

[0037] It can be seen that the attraction of each attractant to the narrow-necked pepper borer is significantly greater than that of a single ingredient and the control. The attractant 5E attracts the largest number of insects and has obvious advantages over other formulas.

[0038] The preferred embodiments of the present invention are described above, but they are not intended to limit the present invention. Those skilled in the art may make improvements and changes to the embodiments disclosed herein without departing from the scope and spirit of the present invention.

Claims

1. A sex pheromone attractant for preventing and controlling adult Zanthoxylum bungeanum, characterized in that: The attractant for adult Zanthoxylum bungeanum in the attractant core comprises cis-7-tetradecenal, palmitic acid and linalyl acetate, and the weight ratio thereof is 10-15:1-2:1-2.

2. The lure according to claim 1, wherein the weight ratio of cis-7-tetradecenal, palmitic acid and linalyl acetate is 10-12:1-1.2:1-1.

2.

3. The lure according to claim 1, wherein the solvent of the Zanthoxylum bungeanum adult attractant in the lure is paraffin oil. The bait according to any one of claims 1 to 3, wherein the carrier of the bait is agar.

5. The preparation method of the sex pheromone attractant for preventing and controlling adult Chinese prickly ash borer according to claim 4, comprising the steps of: weighing agar powder, adding distilled water, and heating to completely dissolve the agar powder; mixing the agar solution while hot with the Chinese prickly ash borer adult attractant, and naturally cooling and solidifying the solution to obtain the attractant.

6. Use of the sex pheromone attractant for controlling adult Zanthoxylum bungeanum according to any one of claims 1 to 4 in controlling Zanthoxylum bungeanum.

7. The use according to claim 6, comprising the steps of placing the prepared lure core into a slow-release bottle, placing a slow-release bottle on each sticky insect board, and hanging the sticky insect boards with the slow-release bottles on the prickly ash trees in the selected plot.

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