Biological feed additive for improving animal intestinal health and improving animal growth performance and preparation method thereof

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a biological feed additive capable of improving animal intestinal health and improving animal growth performance and a preparation method of the biological feed additive. The biological feed additive is prepared from the following raw materials in parts by weight: 20 to 30 parts of zeolite powder, 5 to 10 parts of complex microbial inoculants, 3 to 7 parts of breviscapine, 1 to 4 parts of scoparone, 0.03 to 0.05 part of vitamin E and 0.5 to 1 part of trace elements. When the biological feed additive is added into feed, the intestinal health of animals can be effectively improved, the growth performance of the animals can be improved, the feeding cost can be reduced, and the biological feed additive has a wide application prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of feed additives, and in particular relates to a biological feed additive for improving animal intestinal health and enhancing animal growth performance, and a preparation method thereof. Background Art

[0002] With the expansion and increasing intensification of livestock farming in my country, intestinal diseases in livestock and poultry are becoming increasingly prevalent. According to industry statistics, direct economic losses from intestinal diseases amount to tens of billions of yuan annually. Currently, intestinal diseases in livestock and poultry are primarily caused by intestinal infections caused by bacteria, viruses, and parasites. The widespread use of antibiotics for prevention and treatment has led to a series of serious problems, not only increasing the resistance of pathogens but also causing excessive antibiotic residues in livestock products, posing a serious threat to food safety and human health. Therefore, the search for natural products that can replace antibiotics to prevent and treat intestinal infections is urgent.

[0003] Microbial feed additives refer to microbial products derived from one or more strains of bacteria added to feed. As a new type of biological agent, these products, through the addition of microbial products derived from specific strains, can exert multiple benefits, including supplementing trace elements, regulating the balance of the animal's gastrointestinal flora, inhibiting the growth of pathogenic microorganisms, and enhancing immunity. Therefore, the development of new, environmentally friendly, and highly effective biological feed additives is of great significance.

[0004] Based on the above objectives, the present invention provides a biological feed additive for improving animal intestinal health and enhancing animal growth performance and a preparation method thereof. Summary of the Invention

[0005] The first object of the present invention is to provide a biological feed additive that improves the intestinal health of animals and enhances their growth performance.

[0006] The second object of the present invention is to provide a method for preparing a biological feed additive that improves the intestinal health of animals and enhances the growth performance of animals.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is: A biological feed additive for improving animal intestinal health and enhancing animal growth performance comprises the following raw materials, measured by weight: 20-30 parts of zeolite powder, 5-10 parts of a composite bacterial agent, 3-7 parts of breviscapine aglycone, 1-4 parts of artemisinin, 0.03-0.05 parts of vitamin E, and 0.5-1 parts of trace elements.

[0008] Preferably, the composite bacterial agent is composed of Lactobacillus plantarum liquid, Leuconostoc mesenteroides liquid, and Bacillus natto liquid in a mass ratio of (1-2):(1-2):(1-2).

[0009] Preferably, the specific preparation method of the composite bacterial agent is as follows: (1) Lactobacillus plantarum, Leuconostoc mesenteroides, and Bacillus natto are cultured and fermented separately to obtain Lactobacillus plantarum, Leuconostoc mesenteroides, and Bacillus natto bacterial liquids; (2) Weigh the Lactobacillus plantarum solution, the Leuconostoc mesenteroides solution, and the Bacillus natto solution according to the mass ratio, mix them evenly, and obtain the composite bacterial agent.

[0010] Preferably, the specific steps of the Lactobacillus plantarum fermentation are: inoculating Lactobacillus plantarum into MRS liquid culture medium at an inoculum rate of 3-5 v / v % and culturing at 35-40° C. for 18-24 hours.

[0011] Preferably, the specific step of fermenting Leuconostoc mesenteroides is: inoculating Leuconostoc mesenteroides into MRS liquid culture medium at an inoculum rate of 2-4 v / v % and culturing at 25-30° C. for 24-36 hours.

[0012] Preferably, the specific steps of the Bacillus natto fermentation are: culturing the Bacillus natto in LB liquid culture medium at 35-37° C. for 16-20 hours at an inoculum rate of 1-3 v / v %.

[0013] Preferably, the effective viable counts of Lactobacillus plantarum, Leuconostoc mesenteroides and Bacillus natto in step (1) are all ≥1.0×10 9 CFU / mL.

[0014] Preferably, the trace element is at least one of copper, iron, zinc, manganese, iodine, selenium or calcium.

[0015] According to the above-mentioned method for preparing a biological feed additive for improving animal intestinal health and enhancing animal growth performance, the specific steps include: a. Weigh zeolite powder, composite bacterial agent, breviscapine, scoparia lactone, vitamin E, and trace elements in the above-mentioned proportions by weight and mix well to obtain a premix; b. Add water to the premix obtained in step a to adjust the moisture content to 10-14%, and granulate it using a granulator and dry it to obtain a biological feed additive with a particle size of 3-5 mm.

[0016] Compared with the prior art, the beneficial effects of the present invention are mainly: The present invention provides a biological feed additive for improving intestinal health and growth performance in animals. Zeolite powder is used as a carrier and is combined with a composite bacterial agent, breviscapine, scoparia lactone, vitamin E, trace elements, and other ingredients to help maintain the activity of probiotics. Test results show that adding this biological feed additive to feed can effectively improve intestinal health, enhance growth performance, and reduce feeding costs in animals, suggesting promising application prospects. DETAILED DESCRIPTION

[0017] The technical solution of the present invention will be further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only intended to illustrate the present invention and should not be construed as limiting the present invention. Specific conditions not specified in the examples are to be followed according to conventional conditions or the conditions recommended by the manufacturer. All reagents or instruments used, unless otherwise specified, are conventional products obtained from commercial channels.

[0018] The Lactobacillus plantarum used in the present invention is deposited under the accession number CICC 25125. The Leuconostoc mesenteroides used in the present invention is deposited under the number CICC 6055. The Bacillus natto used in the examples of the present invention has a deposit number of CCTCC KB 20081434.

[0019] Example 1 A biological feed additive for improving animal intestinal health and enhancing animal growth performance comprises the following raw materials, measured in parts by weight: 25 parts of zeolite powder, 7 parts of a composite bacterial agent, 5 parts of breviscapine, 3 parts of artemisinin, 0.045 parts of vitamin E, and 0.7 parts of trace elements; the trace elements are copper, iron, zinc, and manganese mixed in a mass ratio of 1:20:10:5.

[0020] The specific preparation method of the composite bacterial agent is as follows: (1) Lactobacillus plantarum was inoculated into MRS liquid culture medium at a rate of 4 v / v% and cultured at 37°C for 21 hours to obtain a Lactobacillus plantarum bacterial solution; Leuconostoc mesenteroides was inoculated into MRS liquid culture medium at a rate of 3 v / v% and cultured at 27°C for 30 hours to obtain a Leuconostoc mesenteroides bacterial solution; Bacillus natto was inoculated into LB liquid culture medium at a rate of 1-3 v / v% and cultured at 36°C for 18 hours to obtain a Bacillus natto bacterial solution; the effective viable counts of the Lactobacillus plantarum bacterial solution, Leuconostoc mesenteroides bacterial solution, and Bacillus natto bacterial solution were all ≥1.0×10 9 CFU / mL.

[0021] (2) Weigh Lactobacillus plantarum liquid, Leuconostoc mesenteroides liquid, and Bacillus natto liquid in a mass ratio of 1:1:1, mix them evenly, and obtain a composite bacterial agent.

[0022] This embodiment also provides a method for preparing a biological feed additive for improving intestinal health and enhancing growth performance of animals, the specific steps comprising: a. Weigh 25 parts zeolite powder, 7 parts composite bacterial agent, 5 parts breviscapine, 3 parts scoparia lactone, 0.045 parts vitamin E, and 0.7 parts trace elements according to the following weight ratios and mix well to obtain a premix. b. Add water to the premix obtained in step a to adjust the moisture content to 12%, and granulate it using a granulator and dry it to obtain a biological feed additive with a particle size of 4 mm.

[0023] Example 2 A biological feed additive for improving animal intestinal health and enhancing animal growth performance comprises the following raw materials, measured in parts by weight: 20 parts of zeolite powder, 5 parts of a composite bacterial agent, 3 parts of breviscapine, 1 part of artemisinin, 0.03 parts of vitamin E, and 0.5 parts of trace elements; the trace elements are copper, iron, zinc, and manganese mixed in a mass ratio of 1:20:10:5.

[0024] The specific preparation method of the composite bacterial agent is as follows: (1) Lactobacillus plantarum was inoculated into MRS liquid culture medium at a rate of 3 v / v% and cultured at 35°C for 18 hours to obtain a Lactobacillus plantarum bacterial liquid; Leuconostoc mesenteroides was inoculated into MRS liquid culture medium at a rate of 2 v / v% and cultured at 25°C for 24 hours to obtain a Leuconostoc mesenteroides bacterial liquid; Bacillus natto was inoculated into LB liquid culture medium at a rate of 1 v / v% and cultured at 35°C for 16 hours to obtain a Bacillus natto bacterial liquid; the effective viable counts of the Lactobacillus plantarum bacterial liquid, Leuconostoc mesenteroides bacterial liquid, and Bacillus natto bacterial liquid were all ≥1.0×10 9 CFU / mL.

[0025] (2) Weigh Lactobacillus plantarum liquid, Leuconostoc mesenteroides liquid, and Bacillus natto liquid in a mass ratio of 1:1:1, mix them evenly, and obtain a composite bacterial agent.

[0026] This embodiment also provides a method for preparing a biological feed additive for improving intestinal health and enhancing growth performance of animals, the specific steps comprising: a. Weigh 20 parts zeolite powder, 5 parts composite bacterial agent, 3 parts breviscapine, 1 part scoparia lactone, 0.03 parts vitamin E, and 0.5 parts trace elements according to the following weight ratios and mix well to obtain a premix. b. Add water to the premix obtained in step a to adjust the moisture content to 10%, and granulate it using a granulator and dry it to obtain a biological feed additive with a particle size of 3 mm.

[0027] Example 3 A biological feed additive for improving animal intestinal health and enhancing animal growth performance comprises the following raw materials, measured in parts by weight: 30 parts of zeolite powder, 10 parts of a composite bacterial agent, 7 parts of breviscapine, 4 parts of artemisinin, 0.05 parts of vitamin E, and 1 part of trace elements; the trace elements are copper, iron, zinc, and manganese mixed in a mass ratio of 1:20:10:5.

[0028] The specific preparation method of the composite bacterial agent is as follows: (1) Lactobacillus plantarum was inoculated into MRS liquid culture medium at a rate of 5 v / v% and cultured at 40°C for 24 hours to obtain a Lactobacillus plantarum bacterial solution; Leuconostoc mesenteroides was inoculated into MRS liquid culture medium at a rate of 4 v / v% and cultured at 30°C for 36 hours to obtain a Leuconostoc mesenteroides bacterial solution; Bacillus natto was inoculated into LB liquid culture medium at a rate of 3 v / v% and cultured at 37°C for 20 hours to obtain a Bacillus natto bacterial solution; the effective viable counts of the Lactobacillus plantarum bacterial solution, Leuconostoc mesenteroides bacterial solution, and Bacillus natto bacterial solution were all ≥1.0×10 9 CFU / mL.

[0029] (2) Weigh Lactobacillus plantarum liquid, Leuconostoc mesenteroides liquid, and Bacillus natto liquid in a mass ratio of 1:1:1, mix them evenly, and obtain a composite bacterial agent.

[0030] This embodiment also provides a method for preparing a biological feed additive for improving intestinal health and enhancing growth performance of animals, the specific steps comprising: a. Weigh 30 parts zeolite powder, 10 parts composite bacterial agent, 7 parts breviscapine, 4 parts scoparia lactone, 0.05 parts vitamin E, and 1 part trace element according to the following weight ratios and mix well to obtain a premix. b. Add water to the premix obtained in step a to adjust the moisture content to 14%, and granulate it using a granulator and dry it to obtain a biological feed additive with a particle size of 5 mm.

[0031] Comparative Example 1 The difference between Comparative Example 1 and Example 1 is that breviscapine is omitted from the ingredients of the biological feed additive.

[0032] Comparative Example 2 The difference between Comparative Example 1 and Example 1 is that scoparia lactone is omitted from the ingredients of the biological feed additive.

[0033] Comparative Example 3 The difference between Comparative Example 3 and Example 1 is that the artemisinin in the biological feed additive component is replaced by breviscapine.

[0034] Comparative Example 4 The difference between Comparative Example 4 and Example 1 is that Leuconostoc mesenteroides and Bacillus natto in the composite bacterial agent are replaced by Lactobacillus plantarum.

[0035] Test Example 1 The following is an effect test of the biological feed additive for improving animal intestinal health and enhancing animal growth performance obtained in Example 1 and Comparative Examples 1-4: (1) Sixty calves were randomly divided into a control group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, and Comparative Example 4 group, with 10 calves in each group. The experimental calves were of similar age and weight and in good health. Example 1 group: 2% of the weight of the feed was added to the feed of the biological feed additive for improving animal intestinal health and improving animal growth performance prepared in Example 1. The difference between the control group and Example 1 group was that the biological feed additive for improving animal intestinal health and improving animal growth performance prepared in Example 1 was not added to the feed during the feeding process. The other contents such as feeding feed were the same as those of Example 1 group. The remaining groups added 2% of the weight of the feed of the corresponding group of biological feed additives for improving animal intestinal health and improving animal growth performance to the feed. The calves in each group were allowed to eat and drink freely for 30 consecutive days. The calf feed formula, in terms of mass percentage, includes the following raw materials: 53% corn, 15% bran, 30% soybean meal, 1% calcium carbonate, and 1% salt. The fecal scoring standard for calf diarrhea is shown in Table 1. When the fecal score is ≥3, it is recorded as diarrhea. The number of calves with diarrhea is recorded every day according to the fecal scoring standard, and the total number of calves with diarrhea in 30 days is calculated, and the diarrhea rate of calves is calculated; diarrhea rate = [total number of calves with diarrhea / (number of experimental calves × number of experimental days)] × 100%. The results are shown in Table 2.

[0036] Table 1 Stool scoring criteria Table 2 Effects of feed additives on cattle diarrhea The results are shown in Table 2, which shows the effects of the feed additives prepared in Example 1 and Comparative Examples 1-4 on cattle diarrhea. As can be seen from the table, compared with the control group and Comparative Examples 1-4, the biological feed additive for improving animal intestinal health and growth performance prepared in Example 1 of the present invention can significantly improve cattle diarrhea. This shows that the biological feed additive for improving animal intestinal health and growth performance prepared in the present invention, using zeolite powder as a carrier and combined with a composite bacterial agent, breviscapine, scoparia lactone, vitamin E, trace elements and other ingredients, has the effect of improving animal intestinal health, thereby improving animal diarrhea.

[0037] (2) Each group was fed for 60 days. The average initial weight (kg / head) of each group of cattle before the use of feed additives and the average final weight (kg / head) after the use of feed additives for 60 days were recorded, and the average daily weight gain (kg / day) was calculated. The average daily weight gain was calculated using the following formula: average daily weight gain = (final weight - initial weight) / number of feeding days. The results are shown in Table 3.

[0038] Table 3 Effects of feed additives on cattle body weight The results are shown in Table 3, which shows the effects of the feed additives prepared by Example 1 and Comparative Examples 1-4 on cattle weight. As can be seen from the table, compared with the control group and Comparative Examples 1-4, the biological feed additive for improving animal intestinal health and animal growth performance prepared by Example 1 of the present invention can significantly increase the average daily weight gain of cattle. This shows that the biological feed additive for improving animal intestinal health and animal growth performance prepared by the present invention, which uses zeolite powder as a carrier and is combined with a composite bacterial agent, breviscapine, scoparia lactone, vitamin E, trace elements and other ingredients, has the effect of promoting animal growth performance and reducing feeding costs.

[0039] Further analysis shows that compared with Example 1, scutellarin is omitted from the ingredients of the biological feed additive in Comparative Example 1; scoparone is omitted from the ingredients of the biological feed additive in Comparative Example 2; Comparative Example 3 replaces scoparone in the biological feed additive with scutellarin; Comparative Example 4 replaces both Leuconostoc mesenteroides and Bacillus natto in the composite bacterial agent with Lactobacillus plantarum. The effects of the four groups on improving animal intestinal health and improving animal growth performance all declined significantly. This shows that scoparone and scutellarin in the biological feed additive ingredients play a very important role. Only by cooperating with Lactobacillus plantarum, Leuconostoc mesenteroides and Bacillus natto in the composite bacterial agent ingredients can the intestinal health of animals be effectively improved, the growth performance of animals be improved, and the feeding cost be reduced. Moreover, scoparone and scutellarin have a synergistic effect, and the effect is best when used together.

[0040] Finally, it should be noted that the above embodiments are intended only to illustrate the technical solutions of the present invention and are not intended to limit the same. The basic principles and main features of the present invention have been described above using specific implementation schemes. Modifications or substitutions may be made based on the present invention, but such modifications or substitutions do not detract from the essence of the corresponding technical solutions from the scope of protection claimed by the present invention.

Claims

1. A biological feed additive for improving intestinal health and growth performance of animals, characterized in that: The invention comprises the following raw materials in parts by weight: 20-30 parts of zeolite powder, 5-10 parts of compound bacterial agent, 3-7 parts of breviscapine aglycone, 1-4 parts of artemisinin, 0.03-0.05 parts of vitamin E and 0.5-1 parts of trace elements.

2. A biological feed additive for improving animal intestinal health and enhancing animal growth performance according to claim 1, characterized in that: The composite bacterial agent is composed of a Lactobacillus plantarum liquid, a Leuconostoc mesenteroides liquid, and a Bacillus natto liquid in a mass ratio of (1-2):(1-2):(1-2).

3. A biological feed additive for improving animal intestinal health and enhancing animal growth performance according to claim 2, characterized in that: The specific preparation method of the composite bacterial agent is as follows: (1) Lactobacillus plantarum, Leuconostoc mesenteroides, and Bacillus natto are cultured and fermented separately to obtain Lactobacillus plantarum, Leuconostoc mesenteroides, and Bacillus natto bacterial liquids; (2) Weigh the Lactobacillus plantarum solution, the Leuconostoc mesenteroides solution, and the Bacillus natto solution according to the mass ratio, mix them evenly, and obtain the composite bacterial agent.

4. The biological feed additive for improving animal intestinal health and enhancing animal growth performance according to claim 3, characterized in that: The specific steps of the Lactobacillus plantarum fermentation are: inoculating Lactobacillus plantarum into MRS liquid culture medium at an inoculum rate of 3-5 v / v %, and culturing at 35-40° C. for 18-24 hours.

5. The biological feed additive for improving intestinal health and growth performance of animals according to claim 3, characterized in that: The specific steps of the Leuconostoc mesenteroides fermentation are: inoculating Leuconostoc mesenteroides into MRS liquid culture medium at an inoculum rate of 2-4 v / v %, and culturing at 25-30° C. for 24-36 hours.

6. The biological feed additive for improving intestinal health and growth performance of animals according to claim 3, characterized in that: The specific steps of the natto Bacillus subtilis fermentation are as follows: culturing the natto Bacillus subtilis in LB liquid culture medium at 35-37° C. for 16-20 hours at an inoculation rate of 1-3 v / v %.

7. The biological feed additive for improving intestinal health and growth performance of animals according to claim 3, characterized in that: The effective viable counts of the Lactobacillus plantarum, Leuconostoc mesenteroides, and Bacillus natto in step (1) are all ≥1.0×10 9 CFU / mL.

8. The biological feed additive for improving intestinal health and growth performance of animals according to claim 1, characterized in that: The trace element is at least one of copper, iron, zinc, manganese, iodine, selenium or calcium.

9. The method for preparing a biological feed additive for improving intestinal health and growth performance of animals according to any one of claims 1 to 8, characterized in that: The specific steps include: a. Weigh zeolite powder, composite bacterial agent, breviscapine, scoparia lactone, vitamin E, and trace elements in the proportions described in claim 1, mix well, and obtain a premix; b. Add water to the premix obtained in step a to adjust the moisture content to 10-14%, and granulate it using a granulator and dry it to obtain a biological feed additive with a particle size of 3-5 mm.

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