Function enhancer as well as preparation method, use method and application thereof

Abstract

The invention relates to the technical field of feed preparation, in particular to a function enhancer as well as a preparation method, a use method and application thereof. One purpose of the invention is to provide a function enhancer which comprises the following components in parts by weight: 50-100 parts of tortoise shell powder, 5-10 parts of earthworm powder, 2-5 parts of capsicum oleoresin, 2-5 parts of chlorogenic acid, 1-2 parts of 5-hydroxymethylfurfural and 0-60 parts of rice hull powder. The function enhancer provided by the invention is composed of a plurality of natural extracts, including capsicum oleoresin serving as a capsicum extract, chlorogenic acid serving as a green coffee bean extract and 5-hydroxymethylfurfural serving as a prepared rehmannia root extract, and the natural properties of the function enhancer ensure the safety and affinity in a food chain transfer process. Through synergistic interaction of all the components, the absorption and utilization rate of nutrients such as tortoise shell powder colloid, bone salt and earthworm powder amino acid and hyaluronic acid is improved. The enhancer can reduce the incidence rate and severity of dark spots of laying hens in summer.

Description

Technical Field

[0001] The present invention relates to the technical field of feed preparation, and in particular to a functional enhancer and a preparation method, a use method and an application thereof. Background Art

[0002] In the high temperature and humidity of summer, laying hens face a series of physiological stressors, including increased respiratory heat dissipation needs, increased water intake, disrupted nutrient metabolism, deteriorating blood rheology, and reduced oviduct mucosal lubricity. These physiological changes lead to decreased overall feed intake, reduced production performance, and thinning of egg whites, which in turn significantly increase the incidence and severity of dark spots on eggs. Dark spots seriously damage the appearance of eggs, reducing consumer purchases and the market premium for eggs. This results in significant economic losses for farmers and has become a key issue in egg quality control.

[0003] Dark spots on eggs refer to the gray, watermark-like spots that appear on the surface of the eggshell under natural light after the egg is laid. When LED light penetrates, the transmittance of the dark spot area increases, turning it translucent. Dark spots occur less frequently in newly laid eggs, but their incidence gradually increases with storage time, and the size and density of the spots also increase. During storage, the severity of dark spots increases rapidly within 0 to 24 hours after the egg is laid, then the growth rate slows down, entering a relatively stable plateau after 72 hours and no longer deteriorating significantly. This dynamic change characteristic reveals the formation and evolution process of dark spots and is the key basis for researchers to identify the mechanism of dark spots.

[0004] The composition and function of the eggshell membrane have been extensively studied. The eggshell membrane, commonly known as the "phoenix coat," is a network of protein fibers of varying thickness. These proteins primarily consist of keratin, collagen, or elastin analogs, and are rich in key amino acids such as glycine, cystine, proline, and glutamate. In particular, glycine and proline, as the primary components of collagen, play a central role in collagen synthesis and the maintenance of connective tissue structural stability. The tightly interconnected protein fibers of the eggshell membrane connect the egg white and shell, providing excellent breathability and moisture retention, crucial for maintaining the internal environment of the egg.

[0005] However, the root cause of the dark spot problem lies in structural and functional abnormalities in the eggshell and eggshell membrane. Analysis of the dark spot areas revealed enlarged voids in the eggshell membrane fiber network and an abnormally loose structure. This allows water from the egg white to osmotically penetrate the eggshell membrane and seep into the pores of the eggshell, ultimately forming the visible dark spot defect. This structural defect, combined with the physiological disturbances caused by the high temperature and humidity of summer, exacerbates the occurrence of dark spots and highlights the shortcomings of current egg quality management in maintaining the integrity of the eggshell membrane. Although the molecular characteristics of the eggshell membrane have been revealed, effectively preventing and controlling the formation of dark spots to reduce economic losses remains a pressing challenge for the industry.

[0006] In addition, on the one hand, there are differences in understanding among those skilled in the art; on the other hand, the inventor studied a large number of documents and patents when making the present invention, but due to space limitations, not all details and contents are listed in detail. However, this does not mean that the present invention does not have the characteristics of these prior arts. On the contrary, the present invention already has all the characteristics of the prior art, and the applicant reserves the right to add relevant prior art to the background art. Summary of the Invention

[0007] In response to the above technical problems, one of the objectives of the present invention is to provide a functional package technical solution that reduces the incidence and severity of dark spots in eggs laid in summer, thereby improving the production performance of laying hens and the quality of eggs.

[0008] One of the objects of the present invention is to provide a functional enhancer, which comprises, by weight, 50 to 100 parts of tortoise shell powder, 5 to 10 parts of earthworm powder, 2 to 5 parts of chili oleoresin, 2 to 5 parts of chlorogenic acid, 1 to 2 parts of 5-hydroxymethylfurfural and 0 to 60 parts of rice husk powder.

[0009] According to a preferred embodiment, the functional enhancer includes, by weight, 50 parts of tortoise shell powder, 5 parts of earthworm powder, 2 parts of capsicum oleoresin, 2 parts of chlorogenic acid, 1 part of 5-hydroxymethylfurfural and 60 parts of rice husk powder.

[0010] According to a preferred embodiment, the functional enhancer includes, by weight, 100 parts of tortoise shell powder, 10 parts of earthworm powder, 4 parts of capsicum oleoresin, 4 parts of chlorogenic acid and 2 parts of 5-hydroxymethylfurfural.

[0011] According to a preferred embodiment, the functional enhancer includes, by weight, 80 parts of tortoise shell powder, 8 parts of earthworm powder, 5 parts of capsicum oleoresin, 5 parts of chlorogenic acid, 2 parts of 5-hydroxymethylfurfural and 20 parts of rice husk powder.

[0012] According to a preferred embodiment, the tortoise shell powder is a white powder obtained by drying, crushing and passing the plastron and carapace of a tortoise through a 60-mesh sieve, wherein the protein mass percentage is ≥20%, the Ca mass percentage is ≥25%, and the P mass percentage is ≥10%.

[0013] According to a preferred embodiment, earthworm powder is a gray-yellow powder obtained by washing, removing impurities, drying, crushing and passing through a 60-mesh sieve of fresh earthworms, wherein the protein mass proportion is ≥60% and the total amino acid mass proportion is ≥50%.

[0014] According to a preferred embodiment, the capsicum oleoresin is a white powder obtained by washing, leaching and extracting capsicum fruits, mixing the mixture with hot-soluble stearic acid, cooling and crushing the mixture through a 60-mesh sieve, wherein the ratio of capsicum oleoresin to stearic acid is 1:9.

[0015] According to a preferred embodiment, chlorogenic acid is a commercially available natural plant extract of green coffee beans, wherein the mass proportion of chlorogenic acid is ≥50%.

[0016] According to a preferred embodiment, 5-hydroxymethylfurfural is a commercially available natural extract of Rehmannia glutinosa, wherein the mass proportion of 5-hydroxymethylfurfural is ≥20%.

[0017] One of the objectives of the present invention is to provide a method for preparing a functional enhancer, which comprises the following steps: S1 Take 5-hydroxymethylfurfural, chlorogenic acid, capsicum oleoresin and earthworm powder in proportion and mix for 10-15 minutes; S2: Mix the above mixture with tortoise shell powder evenly and set aside.

[0018] Another object of the present invention is to provide a method for breeding chickens, comprising the following steps: feeding the above-mentioned function enhancer or the function enhancer prepared based on the above-mentioned preparation method.

[0019] According to a preferred embodiment, the feeding is carried out in summer or when the temperature reaches above 28°C.

[0020] According to a preferred embodiment, the feeding further comprises a basal feed. Preferably, the basal feed is formulated according to the "Manual for the Management of Commercial Generation of Roman Pink".

[0021] One of the purposes of the present invention is to provide a feed for laying poultry, comprising a function enhancer in an amount of 0.05 to 0.2% by mass.

[0022] According to a preferred embodiment, the egg-laying poultry feed further comprises a basal feed and / or rice hulls. Preferably, the egg-laying poultry feed further comprises 99.8% by weight of the basal feed. Preferably, the egg-laying poultry feed further comprises 99.8% by weight of the basal feed and 0-0.15% by weight of rice hulls.

[0023] Preferably, the egg-laying poultry feed contains 0.1% by mass of the functional enhancer and 99.9% by mass of the basic feed.

[0024] One of the objectives of the present invention is to provide the use of the above-mentioned functional enhancer or the functional enhancer prepared based on the above-mentioned preparation method in reducing dark spots in eggs.

[0025] According to a preferred embodiment, reducing dark spots on eggs means reducing the incidence of dark spots on eggs or reducing the proportion of dark spots on eggs.

[0026] One of the purposes of the present invention is to provide the use of the above-mentioned function enhancer or the function enhancer prepared based on the above-mentioned preparation method in reducing blood lipids in chickens.

[0027] According to a preferred embodiment, the chickens are laying hens.

[0028] Beneficial effects of this technical solution: The functional enhancer provided by the present invention is composed of multiple natural extracts, including capsicum oleoresin (from chili pepper extract), chlorogenic acid (from green coffee bean extract), and 5-hydroxymethylfurfural (from Rehmannia glutinosa extract). Its natural properties ensure safety and compatibility during food chain transmission. The components interact synergistically to enhance the absorption and utilization of nutrients such as tortoise shell powder colloid, bone salt, and amino acids and hyaluronic acid from earthworm powder. This enhancer helps improve blood oxygen supply and oviduct microcirculation in chickens (especially in summer), thereby facilitating nutrient absorption and oviduct lubrication. This effect further promotes protein secretion in the oviduct eggshell membrane, enhancing the water retention capacity of egg white and the water-locking capacity of the eggshell membrane, ultimately reducing the incidence and severity of dark spots in summer laying eggs. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is the scoring standard for dark-spotted eggs, where 1 to 2 points are defined as normal eggs, and 3 to 5 points are defined as dark-spotted eggs. DETAILED DESCRIPTION

[0030] In the description of the present invention, terms are used for descriptive purposes only and should not be understood to indicate or imply relative importance or implicitly indicate the number of technical features indicated. In the description of the present invention, "plurality" means two or more, unless otherwise specifically defined.

[0031] Unless otherwise specified, the test methods used in the following examples are all conventional methods; the materials, reagents or instruments used without indicating the manufacturer are all reagents and materials that can be obtained from commercial channels; if no specific conditions are specified in the examples, they are all carried out according to conventional conditions or conditions recommended by the manufacturer. At the same time, the present invention does not limit the sources of the raw materials used. Unless otherwise specified, the raw materials used in the present invention are all common commercial products in this technical field.

[0032] Example 1 This embodiment relates to a functional enhancer for reducing dark spots in eggs produced in summer.

[0033] The functional enhancer includes 50 parts of tortoise shell powder, 5 parts of earthworm powder, 2 parts of capsicum oleoresin, 2 parts of chlorogenic acid, 1 part of 5-hydroxymethylfurfural, and 60 parts of rice husk powder.

[0034] Example 2 This embodiment relates to a functional enhancer for reducing dark spots in eggs produced in summer.

[0035] The functional enhancer includes 100 parts of tortoise shell powder, 10 parts of earthworm powder, 4 parts of capsicum oleoresin, 4 parts of chlorogenic acid, and 2 parts of 5-hydroxymethylfurfural.

[0036] Example 3 This embodiment relates to a functional enhancer for reducing dark spots in eggs produced in summer.

[0037] The functional enhancer includes 80 parts of tortoise shell powder, 8 parts of earthworm powder, 5 parts of capsicum oleoresin, 5 parts of chlorogenic acid, 2 parts of 5-hydroxymethylfurfural, and 20 parts of rice husk powder.

[0038] Comparative Example 1 The difference from Example 3 is that in this example, chlorogenic acid is replaced by an equal amount of rosmarinic acid.

[0039] Comparative Example 2 Different from Example 3, in this example, an equal amount of feather powder is used to replace earthworm powder.

[0040] Comparative Example 3 Different from Example 3, in this example, an equal amount of pig bone powder is used to replace tortoise shell powder.

[0041] Test Example 1 From May to July 2024, 960 400-day-old New Romanfen laying hens from the same breeding house, batch, and feeding conditions, with similar body weight, were randomly selected from a breeding farm in Zhongjiang, Deyang. The hens were divided into four groups, each containing six replicates and 40 hens per replicate. These groups included Groups 1-3 and a basal diet without functional enhancers, as described in the "Romanfen Commercial Generation Feeding and Management Manual." Groups 1-3 were fed a basal diet supplemented with 0.1% of the corresponding functional enhancers. The breeding houses were modern, enclosed, layer-laying hens maintained a temperature of 28-30°C, a 14-hour daylight period, and a light intensity of 10-15 lux. The hens had free access to food and water. The pre-feeding period lasted one week, and the experimental period lasted five weeks. The production performance of each group was recorded during the experimental period. Egg samples were collected for dark spot scoring, and blood samples were collected after the experiment.

[0042] 1. Based on the feeding trial records, we compiled and analyzed the egg production rate (total number of eggs per week / number of chickens in stock per week), average daily feed intake (weekly feed consumption / weekly number of chickens in stock per week), average egg weight (total egg weight per week / total number of eggs per week), and feed-to-egg ratio (total feed consumption per week / total egg weight per week) for each group. Table 1 shows the production performance during the trial period.

[0043] Table 1

[0044] As shown in Table 1, compared with the basic diet control group, the groups of Examples 1 to 3 can increase the average daily feed intake of the chickens under high temperature conditions in summer, increase the egg production rate of the chickens, reduce the decline in the egg production rate of the chickens, and alleviate the negative impact of high temperature in summer on the production performance of the chickens. The effect of Example 3 is better.

[0045] 2. At the end of the test, collect eggs from all the test chickens and store them under the same conditions (temperature 28℃~30℃, humidity 60%~80%) for 3 days. Then grade the dark spots on the eggs. See the attached diagram for the standard for scoring dark spots on eggs. Figure 1 A score of 1 to 2 was defined as a normal egg, and a score of 3 to 5 was defined as a dark-spotted egg. Table 2 shows the dark-spot scoring results.

[0046] Table 2

[0047] As shown in Table 2, compared with the basic diet control group, the groups of Examples 1 to 3 can reduce the incidence of dark-spotted eggs in chickens under high temperature conditions in summer, and the effect of Example 3 is better.

[0048] 3. After the experiment, the chickens were deprived of feed 4 hours after the experiment, and blood was collected from the wing vein sinus. The serum was centrifuged and separated. The serum samples were tested for lipid metabolism related indicators. Table 3 shows the serum lipid metabolism of chickens in each group.

[0049] Table 3

[0050] As shown in Table 3, compared with the basic diet control group, the groups of Examples 1 to 3 can reduce the total cholesterol and total triglyceride levels in the serum of the chickens under high temperature conditions in summer and increase the high-density lipoprotein cholesterol level in the serum, with Example 2 having the better effect.

[0051] Test Example 2 From June to September 2024, 600 300-day-old Roman Pink laying hens laying dark-spotted eggs were individually caged and numbered, with free access to a basal diet. Egg production was tracked for 15 consecutive days, and eggs were collected and numbered. Eggs were stored for three days at a temperature of 28-30°C and a humidity of 60%-80%, and then scored for dark spots. Based on their dark-spotted egg production, 240 hens with 15 consecutive days of dark-spotted eggs scored 3-5 were selected for the full trial. The experimental hens were randomly divided into five groups, each containing six replicates of eight birds: Example 3, Comparative Examples 1-3, and a dark-spot control group. The dark-spot control group was fed a basal diet based on the experimental basal diet described in the "Roman Pink Commercial Generation Feeding and Management Manual." Groups 3 and Comparative Examples 1-3 were fed a basal diet supplemented with 0.1% of the corresponding functional enhancer. The breeding house is a modern closed laying hen house with an indoor temperature of 28-30 ℃, a lighting time of 14 h, a light intensity of 10-15 lux, free access to food and water, a pre-feeding period of 1 week, and a trial period of 5 weeks.

[0052] 1. Collect egg samples after the test. Store the eggs at a temperature of 28-30°C and a humidity of 60%-80% for 3 days and then score the dark spots on the eggs. See the attached diagram for the standard for scoring dark spots on eggs. Figure 1 A score of 1 to 2 is defined as a normal egg, and a score of 3 to 5 is defined as a dark-spotted egg. Table 4 shows the dark-spotted egg status.

[0053] Table 4

[0054] As shown in Table 4, compared with the dark spot control group, Example 3 can reduce the dark spot score of the dark spot laying hens, reduce the incidence of dark spots, and improve the severity of dark spots in the dark spot laying hens in summer, and the improvement effect is better than that of Comparative Examples 1 to 3.

[0055] 2. After scoring the dark spots, the eggshell membrane was peeled off and its protein and amino acid composition was determined according to GB / T 5009.124-2003 "Determination of Amino Acids in Foods" and GB 5009.5-2010 "National Food Safety Standard - Determination of Protein in Foods". Threonine, valine, isoleucine, leucine, lysine, methionine and phenylalanine were considered essential amino acids, and alanine, valine, isoleucine, leucine, phenylalanine, proline and methionine were considered non-polar hydrophobic amino acids.

[0056] Table 5 shows the protein and amino acid composition of eggshell membranes.

[0057] Table 5

[0058] As shown in Table 5, compared with the dark spot control group, Example 3 can increase the content of glycine and proline in eggshell membrane protein, and improve the protein, total amino acid, essential amino acid and non-polar hydrophobic amino acid content of the eggshell membrane of eggs produced by the dark spot laying hens, and the improvement effect is better than that of Comparative Examples 1 to 3.

[0059] In summary, the functional enhancer of the present invention can improve the production performance and lipid metabolism of laying hens in summer, promote the secretion of eggshell membrane protein in the oviduct of laying hens, increase the glycine and proline content of eggshell membrane fibrin, reduce the incidence of dark spots in eggs of laying hens in summer, improve the severity of dark spots in eggs of laying hens in summer, and thus improve the appearance quality of eggs.

[0060] It should be noted that the above-described specific embodiments are illustrative only. Those skilled in the art may devise various solutions based on the disclosure of the present invention, and such solutions fall within the scope of the disclosure and the scope of protection of the present invention. Those skilled in the art should understand that the present description and drawings are illustrative only and do not constitute limitations of the claims. The scope of protection of the present invention is defined by the claims and their equivalents.

Claims

1. A functional enhancer, characterized in that: The invention comprises, by weight, 50 to 100 parts of tortoise shell powder, 5 to 10 parts of earthworm powder, 2 to 5 parts of capsicum oleoresin, 2 to 5 parts of chlorogenic acid, 1 to 2 parts of 5-hydroxymethylfurfural and 0 to 60 parts of rice husk powder.

2. The functional enhancer according to claim 1, characterized in that By weight, it contains 80 parts of tortoise shell powder, 8 parts of earthworm powder, 5 parts of capsicum oleoresin, 5 parts of chlorogenic acid, 2 parts of 5-hydroxymethylfurfural and 20 parts of rice husk powder.

3. A method for preparing a functional enhancer, characterized in that: The following steps are involved: S1 Take 1-2 parts of 5-hydroxymethylfurfural, 2-5 parts of chlorogenic acid, 2-5 parts of capsicum oleoresin and 5-10 parts of earthworm powder by weight and mix for 10-15 minutes; S2: Evenly mix the above mixture with 50-100 parts of tortoise shell powder and set aside.

4. A poultry feed, characterized in that: The invention comprises 0.05-0.2% by mass of the functional enhancer according to any one of claims 1-2 or the functional enhancer prepared by the preparation method according to claim 4.

5. The poultry feed according to claim 4, characterized in that The poultry feed comprises basal feed and / or rice hulls.

6. A chicken breeding method, characterized in that: The method comprises the following steps: feeding the functional enhancer according to any one of claims 1 to 2 or the functional enhancer prepared by the preparation method according to claim 4.

7. The use of a functional enhancer in reducing dark spots on eggs, characterized in that: The functional enhancer is the functional enhancer according to any one of claims 1 to 2 or the functional enhancer prepared based on the preparation method according to claim 4.

8. The use according to claim 7, characterized in that Reducing dark spots on eggs means reducing the incidence of dark spots on eggs or reducing the proportion of dark spots on eggs.

9. The use of a functional enhancer in reducing blood lipid in chickens, characterized in that: The functional enhancer is the functional enhancer according to any one of claims 1 to 2 or the functional enhancer prepared based on the preparation method according to claim 4.

10. The use according to claim 9, characterized in that The chicken is a laying hen.

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