Optimized preparation method of nanocrystallization lycopene liquid beverage

Abstract

The invention belongs to the technical field of biological and medical preparation, and particularly discloses an optimized preparation method of a nanocrystallized lycopene liquid beverage, which comprises the following steps: after a lycopene stock solution is subjected to nanoscale purification and centrifugal filtration, adding a glycosyl surfactant, and performing nano co-stirring to obtain the nanocrystallized lycopene liquid beverage. After ultrasonic treatment, the temperature of the liquid is controlled to be 20-25 DEG C through ice bath; therefore, the glycosyl surfactant-lycopene liquid drink with a stable dispersion system is obtained. The particle size of the nanoparticles of the glycosyl surfactant synergistic lycopene liquid drink prepared by the invention is distributed between 50 and 400nm, the stability is good, and the lycopene liquid drink has the advantages of good taste, oxidation resistance, inflammation resistance, cancer resistance and the like which are not possessed by a lycopene stock solution; the nano-particle preparation of the mannose, dextran and lycopene liquid drink prepared by the invention is suitable for being taken as an oral preparation, and has the advantages of beautifying, improving immunity, and being low in toxicity and good in health-care effect.

Description

Technical Field

[0001] The present invention belongs to the field of biological and pharmaceutical preparation technology, and particularly relates to an optimized preparation method of a nano-sized lycopene liquid beverage. Background Art

[0002] Amidst the ongoing innovation and development in biotechnology and pharmaceutical preparation technologies, functional beverages, with their unique health properties, have become a hot research and development topic and a market focus. Lycopene liquid beverage, a prime example of this category, stands out thanks to its environmentally friendly and healthy production process. Only two additives, citric acid and sucralose, are added during production, ensuring harmless additions. Furthermore, a specialized sterilization process ensures a high level of food safety. In recent years, this product has garnered widespread attention in both the market and scientific research communities.

[0003] Lycopene, a natural red hydrocarbon carotenoid, demonstrates significant potential for maintaining human health. Its exceptional antioxidant properties effectively scavenge free radicals in the body and significantly reduce cellular damage caused by oxidative stress. Numerous studies have demonstrated that this antioxidant activity plays a key role in important areas such as cancer prevention and anti-aging. Consequently, lycopene is widely used in a variety of applications, including food, pharmaceuticals, and cosmetics. In the food industry, for example, numerous companies are incorporating it into various products to enhance their nutritional value. In the cosmetics sector, skincare products containing lycopene claim to have antioxidant and anti-aging benefits, attracting consumers. However, lycopene itself exhibits significant drawbacks, significantly hindering its broader and more comprehensive application. Under the influence of various external factors, such as light, temperature, and metal ions, lycopene is highly susceptible to oxidative decomposition. Once this occurs, the product's signature red color gradually fades and turns yellow, severely impacting its appearance and reducing consumer purchase intent. It also significantly reduces the content of its active ingredient, diminishing its functional activity. In actual production and application, this problem shortens the shelf life of lycopene liquid drinks and makes their efficacy unstable, making it difficult to meet the market demand for high-quality, long-lasting functional drinks, and severely limiting their stability and effectiveness.

[0004] In order to effectively overcome the above-mentioned defects of lycopene and further improve the nutritional value and immune functionality of lycopene liquid drinks, developing a new optimized preparation method for nano-sized lycopene liquid drinks has become a key issue that needs to be urgently addressed in the industry. Summary of the Invention

[0005] The purpose of the present invention is to overcome the defects of the existing technology and provide an optimized preparation method for nano-sized lycopene liquid beverage. The sugar-based surfactant is combined with the liquid beverage carrier by self-assembly, and nanoparticles of carbonyl surfactant-coordinated lycopene liquid beverage are obtained through intermolecular interactions (such as hydrogen bonds, π-π stacking, hydrophobic effects, etc.). Lycopene modified with sugars enters the body as a drug carrier, which can effectively increase the water solubility of lycopene and enable it to be stably absorbed in the intestines; thereby interacting with beneficial intestinal microorganisms and protecting the stability and activity of the intestinal flora; when lycopene is exposed to oxidants or free radicals, the double bonds can be cleaved or increased, thereby destroying the polyene chain. The nano-sized lycopene drug delivery system can avoid the loss of activity of the active ingredient and facilitate the precise transportation of the drug to various parts of the human body.

[0006] To achieve the above objectives, one of the technical solutions of the present invention is: an optimized preparation method for a nano-sized lycopene liquid beverage, comprising the following steps: subjecting a lycopene stock solution to nano-scale purification and centrifugal filtration, adding a sugar-based surfactant, using nano-co-stirring, and then ultrasonically treating the liquid and controlling the liquid temperature in an ice bath within the range of 20-25°C, thereby obtaining a sugar-based surfactant-lycopene liquid beverage with a stable dispersion.

[0007] In a preferred embodiment of the present invention, the nano-scale purification centrifuge speed is 8000-10000 rpm, the time is 10-15 min, and the pore size of the nano-scale filter is 600-1000 nm.

[0008] In a preferred embodiment of the present invention, the sugar-based surfactant is added in an amount of 3-7 vol% of the lycopene stock solution.

[0009] In a preferred embodiment of the present invention, the rotation speed of the nano-co-stirring is 500-700 rpm, the temperature is room temperature, and the time is 4-10 hours.

[0010] In a preferred embodiment of the present invention, the ultrasonic treatment power is 400-600 W, and the ultrasonic treatment time is 10-15 min.

[0011] In a preferred embodiment of the present invention, the sugar-based surfactant is at least one of mannose, dextran, β-D-galactose pentaacetate, N-ethyl-D-glucosamine and sucrose octaacetate.

[0012] Sugars are organic compounds composed of polyhydroxy aldehydes, polyhydroxy ketones, and hydrolyzable polyhydroxy aldehydes and polyhydroxy ketones, and are divided into monosaccharides, disaccharides, and polysaccharides. The glycoside surfactants in the present invention, such as mannose, dextran, N-ethyl-D-glucosamine, sucrose octaacetate, β-D-galactose pentaacetate, etc., have antibacterial activity, antitumor activity, high biocompatibility, and biodegradability. They are generally composed of a hydrophilic glycosyl group (glucose, sucrose, and maltose, etc.) and a hydrophobic alkyl carbon chain, and have large structural diversity; their properties can be changed by adjusting the sugar head group or non-polar tail chain, so glycoside surfactants have huge application potential in the pharmaceutical and food industries. Among them, mannose (Mannose) and dextran (Dextran) are functionally diverse glycoside surfactants in life. They are converted in the body through the sugar metabolism pathway and play an important role in the immune system. For example, they have functions such as providing energy, anti-cancer, lowering blood sugar, anti-aging, and enhancing immunity, and are therefore widely used in health foods and medicines. From a biological perspective, mannose plays a role in the human immune system, particularly in disease resistance and bacterial recognition; for example, it helps the body identify and eliminate specific pathogens. Mannose is also a key component in the synthesis of glycoproteins (such as glycosaminoglycans and glycosylated proteins), which play a role in cell-cell interactions and recognition. Dextran is primarily derived from plants and fruits, such as blueberries and oranges, and found in the seeds and rhizomes of plants like chicory and soybeans. Dextran is widely marketed as a dietary supplement. Some bacteria considered "gut probiotics," such as certain bifidobacteria and lactic acid bacteria, can also produce and utilize dextran. These bacteria participate in the conversion of carbohydrates through metabolic pathways, affecting the intestinal flora and potentially influencing insulin and glucagon sensitivity. Recent studies have demonstrated that dextran wound dressings have good clinical value in the treatment of erythematotelangiectatic rosacea and can also be used to prevent urinary tract infections.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] 1. The lycopene liquid drink prepared by the present invention is based on a sugar-based surfactant and has good stability and excellent biocompatibility. It can exert targeted anti-inflammatory and anti-cancer effects, has outstanding value in food storage, and has advantages that bare drugs do not have, such as non-toxicity, long-term effectiveness, and high efficiency.

[0015] 2. The nanoemulsion prepared by using the sugar-based surfactant prepared in the present invention in conjunction with the lycopene liquid drink is suitable for use as an oral beverage and has the advantages of good taste, low toxicity and good therapeutic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a transmission electron micrograph of the mannose-lycopene liquid beverage nanoparticles in Example 1;

[0017] Figure 2 This is the infrared spectrum of the mannose-lycopene liquid drink nanoparticles in Example 1;

[0018] Figure 3 This is a transmission electron micrograph of the nanoparticles of the dextran synergistic lycopene liquid drink in Example 2;

[0019] Figure 4 This is the infrared spectrum of the nanoparticles of the dextran synergistic lycopene liquid drink in Example 2;

[0020] Figure 5 This is a transmission electron micrograph of the nanoparticles of β-D-galactose pentaacetate synergistically with lycopene in the liquid drink in Example 3;

[0021] Figure 6 This is the infrared spectrum of the nanoparticles of β-D-galactose pentaacetate synergistic with lycopene liquid drink in Example 3;

[0022] Figure 7 This is a transmission electron micrograph of the nanoparticles of the N-ethyl-D-glucosamine synergistic lycopene liquid drink in Example 4;

[0023] Figure 8 This is an infrared spectrum of the nanoparticles of N-ethyl-D-glucosamine synergistically formulated with lycopene in the liquid drink in Example 4;

[0024] Figure 9 This is a transmission electron micrograph of nanoparticles of sucrose octaacetate synergistically with lycopene in the liquid drink in Example 5;

[0025] Figure 10 This is the infrared spectrum of the nanoparticles of sucrose octaacetate synergistically with lycopene in the liquid drink in Example 5. DETAILED DESCRIPTION

[0026] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is described in more detail below with reference to specific embodiments, but the protection scope of the present invention is not limited to these embodiments.

[0027] The lycopene liquid drink described in this invention is made from tomatoes unique to the Xinjiang Uyghur Autonomous Region. These tomatoes experience up to 18 hours of sunshine, giving the product a sweet and mellow taste and rich nutritional profile. During production, only citric acid and sucralose are added as harmless additives. A specialized sterilization process ensures a high level of food safety. Furthermore, the liquid drink undergoes micron-level processing, resulting in particles smaller than 2 μm, significantly improving the body's absorption of its nutrients. Rich in energy and protein, it is a model of a green and healthy beverage.

[0028] The Chinese and English names of the sugar-based surfactants of the present invention correspond to the following:

[0029] Mannose: Mannose, molecular formula (Hill notation) is C6H 12 O6;

[0030] Dextran: Dextran, molecular formula (Hill notation) is C6H 12 O6

[0031] β-D-Galactose pentaacetate: Beta-D-Galactose pentaacetate; β-D-Gal-5-Ac, molecular formula (Hill notation) C 16 H 22 O 11

[0032] N-Ethyl-D-glucamine: N-Ethyl Glucamine; N-Et-GlcN, molecular formula (Hill notation) C8H 19 NO5

[0033] Sucrose octaacetate: Sucrose octaacetate; SOA, molecular formula (Hill notation) C28H 38 O 19

[0034] An optimized preparation method for a nano-sized lycopene liquid beverage comprises the following steps: subjecting a lycopene stock solution to nano-scale purification and centrifugal filtration, adding a sugar-based surfactant, using nano-co-stirring, and then ultrasonically treating the solution and controlling the liquid temperature in an ice bath within the range of 20-25°C, thereby obtaining a sugar-based surfactant-lycopene liquid beverage with a stable dispersion system.

[0035] The nano-scale purification centrifugal speed is 8000-10000 rpm, the time is 10-15 minutes, and the pore size of the nano-scale filter is 600-1000 nm.

[0036] The added amount of the sugar-based surfactant is 3-7 vol% of the lycopene stock solution.

[0037] The rotation speed of the nano-co-stirring is 500-700 rpm, the temperature is room temperature, and the time is 4-10 hours.

[0038] The ultrasonic treatment power is 400-600W, and the ultrasonic treatment time is 10-15 minutes.

[0039] The sugar-based surfactant is at least one of mannose, dextran, β-D-galactose pentaacetate, N-ethyl-D-glucosamine and sucrose octaacetate.

[0040] The lycopene stock solution (LYC) used in the following examples was prepared by pouring 50 L of a lycopene liquid beverage (made from tomatoes native to the Xinjiang Uyghur Autonomous Region, with only citric acid and sucralose added) into a container and centrifuging at 8000 rpm for 10 minutes to remove large particles and impurities. The centrifuged liquid is the lycopene stock solution and does not require further filtration.

[0041] Example 1

[0042] A nanoemulsion for a mannose-lycopene liquid beverage comprises the following steps: purifying a lycopene stock solution using an 800 nm filter, adding 2.5 mg of mannose to 50 L of the lycopene stock solution, and stirring the solution with a magnetic stirrer at 700 rpm, maintaining the reaction temperature at room temperature, for 4 hours to ensure that the mannose is evenly dissolved and dispersed in the lycopene liquid beverage. Subsequently, the treated solution is ultrasonically treated using a 600 W ultrasonic processor in a pulse mode (2 seconds on, 1 second off) for 10 minutes. The liquid temperature is maintained at 23°C in an ice bath to promote uniformity and stability.

[0043] Figure 1 This is a transmission electron micrograph of the nanoparticles of the mannose-lycopene liquid drink of this example; Figure 2 This is the infrared spectrum of the nanoparticles of the mannose-lycopene liquid drink of this embodiment. Figure 1 、 Figure 2 The physicochemical characterization of mannose synergistic lycopene liquid drink nanoemulsion was known.

[0044] Example 2

[0045] A nanoemulsion containing dextran and lycopene in a liquid beverage is prepared by the following steps: purifying a lycopene stock solution using an 800 nm filter, adding 2.5 mg of dextran to 50 L of the lycopene stock solution, stirring the solution with a magnetic stirrer at a speed of 500-700 rpm while maintaining the reaction temperature at room temperature for 4 minutes to ensure that the dextran is uniformly dissolved and dispersed in the lycopene liquid beverage. Subsequently, the treated solution is ultrasonically treated using a 600 W ultrasonic processor in a pulse mode (2 seconds on, 1 second off) for 10 minutes. The liquid temperature is maintained at 23°C in an ice bath to promote uniformity and stability.

[0046] Figure 3 This is a transmission electron micrograph of the nanoparticles of the dextran synergistic lycopene liquid drink of this example; Figure 4 This is the infrared spectrum of nanoparticles of dextran synergistic lycopene liquid drink. Figure 3 、 Figure 4 The physicochemical characterization of dextran synergistic lycopene liquid drink nanoemulsion was known.

[0047] Example 3

[0048] A nanoemulsion containing β-D-galactose pentaacetate and lycopene in a liquid beverage is prepared by the following steps: purifying a lycopene stock solution using an 800 nm filter, adding 2.5 mg of β-D-galactose pentaacetate to 50 L of the lycopene stock solution, stirring the solution with a magnetic stirrer at 700 rpm while maintaining the reaction temperature at room temperature for 4 hours to ensure that the β-D-galactose pentaacetate is uniformly dissolved and dispersed in the lycopene liquid beverage. Subsequently, the treated solution is ultrasonically treated using a 600 W ultrasonic processor in a pulse mode (2 seconds on, 1 second off) for 10 minutes. The liquid temperature is controlled to below 23°C in an ice bath to promote liquid homogeneity and stability.

[0049] Figure 5 This is a transmission electron micrograph of nanoparticles of β-D-galactose pentaacetate synergistic with lycopene in the liquid drink of this example; Figure 6 This is the infrared spectrum of the nanoparticles of β-D-galactose pentaacetate synergistic with lycopene liquid drink in this embodiment. Figure 5 、 Figure 6 It can be seen that the physicochemical characterization of β-D-galactose pentaacetate synergistically with lycopene liquid drink nanoemulsion.

[0050] Example 4

[0051] A nanoemulsion containing N-ethyl-D-glucosamine and lycopene in a liquid beverage is prepared by the following steps: purifying a lycopene stock solution using an 800 nm filter, adding 2.5 mg of N-ethyl-D-glucosamine to 40-50 L of the lycopene stock solution, and stirring the mixture using a magnetic stirrer at 700 rpm while maintaining the reaction temperature at room temperature for 4 hours to ensure that the N-ethyl-D-glucosamine is uniformly dissolved and dispersed in the lycopene liquid beverage. Subsequently, the treated solution is ultrasonically treated using a 600 W ultrasonic processor in a pulse mode (2 seconds on, 1 second off) for 10 minutes. The liquid temperature is maintained below 25°C in an ice bath to promote uniformity and stability.

[0052] Figure 7 This is a transmission electron micrograph of the nanoparticles of the N-ethyl-D-glucosamine synergistic lycopene liquid drink of this example; Figure 8 This is the infrared spectrum of the nanoparticles of N-ethyl-D-glucosamine synergistically prepared lycopene liquid drink in this embodiment. Figure 7 、 Figure 8 It can be seen that the physicochemical characterization of N-ethyl-D-glucosamine synergistically with lycopene liquid drink nanoemulsion.

[0053] Example 5

[0054] A nanoemulsion containing sucrose octaacetate and lycopene in a liquid beverage is prepared by the following steps: purifying a lycopene stock solution using an 800 nm filter, adding 2.5 mg of sucrose octaacetate to 50 L of the lycopene stock solution, and stirring the solution with a magnetic stirrer at 700 rpm, maintaining the reaction temperature at room temperature, for 4 hours to ensure that the sucrose octaacetate is uniformly dissolved and dispersed in the lycopene liquid beverage. Subsequently, the treated solution is ultrasonically treated using a 600 W ultrasonic processor in a pulse mode (2 seconds on, 1 second off) for 10 minutes. The liquid temperature is maintained below 25°C in an ice bath to promote uniformity and stability.

[0055] Figure 9 This is a transmission electron micrograph of nanoparticles of sucrose octaacetate synergistic with lycopene in the liquid drink of this example; Figure 10 This is the infrared spectrum of nanoparticles of sucrose octaacetate synergistically with lycopene in liquid drink. Figure 9 、 Figure 10 It can be seen that the physicochemical characterization of sucrose octaacetate synergistically with lycopene liquid drink nanoemulsion.

[0056] The nanoparticles of the sugar-based surfactant-synergistic lycopene liquid drink prepared by the present invention have a particle size distribution between 50 and 400 nm, good stability, and have advantages that lycopene stock solution does not have, such as good taste, antioxidant, anti-inflammatory and anti-cancer properties; the nanoparticle preparation of mannose and dextran-synergistic lycopene liquid drink prepared by the present invention is suitable for use as an oral preparation, and has the advantages of nourishing the skin, improving immunity, low toxicity and good health care effects.

[0057] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An optimized preparation method for a nano-lycopene liquid beverage, characterized in that: The following steps are involved: The lycopene stock solution is purified by nano-scale centrifugation, and then a sugar-based surfactant is added. After nano-co-stirring, the liquid temperature is controlled within the range of 20-25°C by ultrasonic treatment and ice bath, thereby obtaining a sugar-based surfactant-lycopene liquid drink with a stable dispersion system.

2. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The nano-scale purification centrifugal speed is 8000-10000 rpm.

3. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The nano-scale purification centrifugation time is 10 to 15 minutes.

4. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The pore size of nano-filter is 600~1000nm.

5. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The added amount of the sugar-based surfactant is 3-7 vol% of the lycopene stock solution.

6. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The rotation speed of the nano-co-stirring is 500-700 rpm.

7. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The nanometer is stirred for 4 to 10 hours.

8. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The ultrasonic treatment power is 400-600W.

9. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The ultrasonic treatment time is 10 to 15 minutes.

10. The optimized preparation method of the nano-lycopene liquid beverage according to claim 1, characterized in that: The sugar-based surfactant is at least one of mannose, dextran, β-D-galactose pentaacetate, N-ethyl-D-glucosamine and sucrose octaacetate.

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