Peanut sprout water extraction enriched product, preparation method and application of peanut sprout water extraction enriched product in sleep aiding composition

Abstract

The invention discloses a peanut sprout water extraction enriched product, a preparation method and application of the peanut sprout water extraction enriched product in a sleep-aiding composition. The content of resveratrol in the peanut sprout water extraction enriched product is 0.8-1.5 mg / g, the content of total amino acid is 100-200 mg / g, the content of tryptophan is 0.24-0.30 mg / g, and the molar ratio of tryptophan to magnesium metal is 1: (0.4-0.6). The content of resveratrol in the obtained peanut sprout water extraction enriched product is high, the peanut sprout water extraction enriched product has a directional enrichment effect on tryptophan and metal magnesium ions, and research proves that the peanut sprout water extraction enriched product has a remarkable effect on improving sleep disorders.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a peanut sprout water extract enrichment, a preparation method and application of the peanut sprout water extract enrichment in a sleep-aiding composition. Background Art

[0002] Gamma-aminobutyric acid (GABA) is an important inhibitory neurotransmitter. Numerous studies have shown that dysfunction of the GABAergic system is closely related to insomnia and affective disorders such as anxiety and depression. Neuropsychological abnormalities such as anxiety and depression may be one of the causes of insomnia. Current mainstream sleep aids have significant limitations: 1. Chemically synthesized drugs (such as benzodiazepines and Z drugs): Although they can quickly induce sleep, long-term use can easily lead to drug dependence, cognitive impairment, and rebound insomnia. Clinical data show that the rate of tolerance to sleeping pills in patients who continue to use them for more than 4 weeks is as high as 67%; 2. Natural plant extracts: Ingredients such as jujube saponins and valerian triesters require milligram-level doses to be effective, and their sour and bitter taste seriously affects palatability. Melatonin products have the risk of disrupting circadian rhythms, and the EU EFSA stipulates that daily intake should not exceed 1 mg. 3. Nutritional supplements: When used alone, commercially available sedatives such as γ-aminobutyric acid and glycine have limited practical effects due to their low blood-brain barrier permeability (<15%) and rapid metabolic rate.

[0003] Peanut sprouts are an emerging functional food raw material. Studies have confirmed that they contain a variety of antioxidants such as resveratrol and polyphenols. In addition, the content of amino acids and trace elements in peanut sprouts is much greater than that of sprouted beans of general beans, and the rich tryptophan can regulate nerves and stabilize emotions, thereby helping to improve sleep. Therefore, the development of new preparations with peanut sprout enrichment as the main active ingredient is of great significance for the research on improving sleep disorders. However, there are two major technical bottlenecks in the existing extraction process, which leads to its weak functionality in the field of sleep aid: 1. Low utilization rate of active ingredients: traditional drying and crushing processes destroy heat-sensitive amino acids, and the tryptophan retention rate is less than 0.3%; 2. Key elements are not synergistic, and the existing preparation method only achieves an increase in the total amino acid content, and does not solve the problem of optimizing the ratio of specific ingredients. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention first proposes a peanut sprout water extract enrichment, which has an ideal amino acid content and a specific source tryptophan / metal magnesium ratio. Studies have shown that it is more conducive to exerting the synergistic mechanism of neural regulation and achieving the optimization of the ratio of specific ingredients for sleep-inducing.

[0005] Based on the second aspect of the present invention, a method for preparing a peanut sprout water extract enrichment is also proposed. Through directional adsorption of a specific resin and a specific spray drying process, not only is the content of resveratrol in the obtained peanut sprout water extract enrichment high, but it also has a directional enrichment effect on tryptophan and metal magnesium ions.

[0006] According to the third aspect of the present invention, a sleep-aiding composition is also provided. The composition comprises the above-mentioned peanut sprout water extract and honey as main ingredients, has an ideal sleep-aiding effect, and has good bioavailability.

[0007] To achieve the above object, the technical solutions adopted by the present invention are as follows: First, a peanut sprout water extract concentrate is provided, wherein the resveratrol content is 0.8-1.5 mg / g, the total amino acid content is 100-200 mg / g, the tryptophan content is 0.24-0.30 mg / g, and the molar ratio of tryptophan to metal magnesium is 1:(0.4-0.6).

[0008] The above-mentioned peanut sprout water extract enrichment has a specific ratio of tryptophan / metallic magnesium. Studies have confirmed that it can exert typical functional synergy through a neuromodulatory synergistic mechanism, thereby showing significant advantages in improving sleep disorders.

[0009] Secondly, a method for preparing the peanut sprout water extract concentrate as described above is also provided, comprising the following steps: 1) Dissolve peanut sprouts in water, extract to obtain an aqueous solution, and adjust the pH to 2-3; 2) The aqueous solution is passed through an ion exchange column for adsorption, and then eluted with an eluent to obtain an extract; the extract is spray-dried to obtain a powder, which is the peanut sprout water extract concentrate; The ion exchange column is a series connection of a strongly acidic cation exchange resin and a macroporous adsorption resin, preferably a series connection of a 732 resin and a HPD-100 resin.

[0010] As some preferred examples of the present invention, the method for preparing the peanut sprouts is: soaking the peanuts in water for 8-48 hours, and then cultivating them in an environment of 20-28° C. and 85-92% humidity for 5-6 days; cutting off the roots of the peanut sprouts and crushing them.

[0011] As some preferred examples of the present invention, the water extraction conditions in step 1) are: shaking at 60-80° C. for 12-48 hours, and collecting the filtrate.

[0012] As some preferred examples of the present invention, the adsorption conditions in step 2) are: adsorption temperature 20-40° C., preferably 25-35° C., and filtrate flow rate 1-3 Bv / h.

[0013] As some preferred examples of the present invention, the 732 resin is adsorbed and eluted with 1-3% ammonia water, and the eluate containing amino acids and magnesium ions is collected; Preferably, after adsorption on the HPD-100 resin, elution is performed using 60-80% ethanol, and the eluate containing resveratrol is collected.

[0014] As some preferred examples of the present invention, the main process parameters of the spray drying are: inlet air temperature: 160-180°C; outlet air temperature: 80-90°C; atomization pressure: 0.2-0.4 MPa; feed flow rate: 5-10 mL / min.

[0015] The preparation method provided by the present invention uses 732 resin and HPD-100 resin in series to carry out targeted adsorption of peanut sprout water extract, combined with a spray drying process to retain thermosensitive amino acids and increase the tryptophan content. Not only is the content of active ingredients in the enriched product high, but the molar ratio of tryptophan to metallic magnesium can be controlled within the range of 1:(0.4-0.6). The enriched product has a significant effect in sleep-inducing functional foods or dietary supplements.

[0016] Furthermore, a sleep-aiding composition is provided, comprising honey and a peanut sprout water extract concentrate; the peanut sprout water extract concentrate is the peanut sprout water extract concentrate described above or the peanut sprout water extract concentrate prepared by the method described above; The mass ratio of the peanut sprout water extract concentrate to honey is 1:(10-20).

[0017] As some preferred examples of the sleep-aiding composition of the present invention, the honey is selected from at least one of acacia flower honey, jujube flower honey or vitex honey.

[0018] Finally, the present invention also provides a use of the sleep-aiding composition as described above in a sleep-aiding functional food or dietary supplement.

[0019] The present invention solves the aforementioned problems in a breakthrough way through the following paths: (1) A peanut sprout water extract concentrate was proposed. It not only contains high levels of resveratrol, amino acids and other nutrients, but also has a high content of tryptophan, and the molar ratio of tryptophan to magnesium ions is 1:(0.4-0.6). The study found that the peanut sprout water extract concentrate with this specific ingredient ratio has a significant effect on improving sleep disorders.

[0020] (2) Directed enrichment process: Through selective adsorption of specific resin, a peanut sprout water extract enrichment with a source tryptophan / magnesium ion molar ratio of 1: (0.4-0.6) can be obtained. For the first time, it was revealed that the above ratio range is positively correlated with the activation efficiency of the GABAergic system, which shows a significant effect on improving sleep disorders.

[0021] (3) Dosage form optimization: Honey is innovatively introduced as a natural delivery system. Its rich fructose / glucose complex can promote intestinal amino acid transporter (PAT1)-mediated absorption, solve the problem of amino acid components being easily oxidized and inactivated, and at the same time mask the unpleasant flavor of plant extracts, and improve bioavailability. DETAILED DESCRIPTION

[0022] The present invention will be further described below through specific examples. The examples of the present invention are only for illustration of the present invention and do not limit the scope of the present invention.

[0023] In the following examples of the present invention, resveratrol content was analyzed by HPLC chromatography, amino acid content was tested by an automatic amino acid analyzer, and magnesium ion content was analyzed by atomic absorption spectrometry. HPLC chromatography conditions: C18 column, acetonitrile-water gradient elution. Example 1

[0024] A method for preparing a peanut sprout water extract concentrate comprises the following steps: a. Prepare peanut sprouts: Soak the peanuts in water for 12 hours, then incubate them in a dark environment at 25°C and 89% humidity for 6 days. Cut off the roots of the peanut sprouts and crush them into powder.

[0025] b. Take 10 g of the peanut sprouts obtained in step a and dissolve them in 50 ml of water, and extract them with water to obtain an aqueous solution; the extraction conditions are: shaking at 60°C for 48 hours, collecting the filtrate, and adjusting the pH to 2-3.

[0026] c. The filtrate was sequentially passed through an ion exchange column filled with 732 resin and HPD-100 resin for adsorption at an adsorption temperature of 35°C and a filtrate flow rate of 1 Bv / h. After adsorption by 732 resin, elution was performed with 2% ammonia water. After adsorption by HPD-100 resin, elution was performed with 70% ethanol. The eluates were combined. The eluates were spray-dried. The main process parameters were: inlet air temperature: 160°C; outlet air temperature: 80°C; atomization pressure: 0.2 MPa; feed flow rate: 5 mL / min. The obtained powder was recorded as peanut sprout aqueous extract enrichment A.

[0027] Analysis showed that the peanut sprout water extract obtained in this example had a resveratrol content of 1.1 mg / g, a total amino acid content of 160 mg / g, a tryptophan content of 0.3 mg / g, and a molar ratio of tryptophan to magnesium of 1:0.5. Example 2

[0028] A method for preparing a peanut sprout water extract concentrate comprises the following steps: a. Prepare peanut sprouts: Soak the peanuts in water for 32 hours, then incubate them in a dark environment at 20°C and 92% humidity for 5 days; cut off the roots of the peanut sprouts and crush them.

[0029] b. Take 10 g of the peanut sprouts obtained in step a and dissolve them in 50 ml of water, and extract them with water to obtain an aqueous solution; the extraction conditions are: shaking at 80°C for 12 hours, collecting the filtrate, and adjusting the pH to 2-3.

[0030] c. The filtrate was sequentially passed through an ion exchange column filled with 732 resin and HPD-100 resin for adsorption at a temperature of 25°C and a filtrate flow rate of 2.5 Bv / h. After adsorption by 732 resin, elution was performed with 2% ammonia water. After adsorption by HPD-100 resin, elution was performed with 70% ethanol. The eluates were combined. The eluates were spray-dried. The main process parameters were: inlet air temperature: 180°C; outlet air temperature: 90°C; atomization pressure: 0.4 MPa; feed flow rate: 10 mL / min. The obtained powder was recorded as peanut sprout aqueous extract enrichment B.

[0031] Analysis showed that the peanut sprout water extract obtained in this example had a resveratrol content of 1.5 mg / g, a total amino acid content of 110 mg / g, a tryptophan content of 0.26 mg / g, and a molar ratio of tryptophan to magnesium of 1:0.42. Example 3

[0032] A method for preparing a peanut sprout water extract concentrate comprises the following steps: a. Prepare peanut sprouts: Soak the peanuts in water for 24 hours, then incubate them in a dark environment at 28°C and 85% humidity for 6 days; cut off the roots of the peanut sprouts and crush them into powder.

[0033] b. Take 10 g of the peanut sprouts obtained in step a and dissolve them in 50 ml of water, and extract them with water to obtain an aqueous solution; the extraction conditions are: shaking at 70°C for 24 hours, collecting the filtrate, and adjusting the pH to 2-3.

[0034] c. The filtrate was sequentially passed through an ion exchange column filled with 732 resin and HPD-100 resin for adsorption at an adsorption temperature of 40°C and a filtrate flow rate of 3 Bv / h. After adsorption by 732 resin, elution was performed with 2% ammonia water. After adsorption by HPD-100 resin, elution was performed with 70% ethanol. The eluates were combined. The eluates were spray-dried. The main process parameters were: inlet air temperature: 170°C; outlet air temperature: 85°C; atomization pressure: 0.3 MPa; feed flow rate: 7 mL / min. The obtained powder was recorded as peanut sprout aqueous extract enrichment C.

[0035] Analysis showed that the peanut sprout water extract obtained in this example had a resveratrol content of 1.2 mg / g, a total amino acid content of 130 mg / g, a tryptophan content of 0.28 mg / g, and a molar ratio of tryptophan to magnesium of 1:0.6. Example 4

[0036] A method for preparing a peanut sprout water extract concentrate comprises the following steps: a. Prepare peanut sprouts: Soak the peanuts in water for 48 hours, then incubate them in a dark environment at 23°C and 90% humidity for 5 days; cut off the roots of the peanut sprouts and crush them.

[0037] b. Take 10 g of the peanut sprouts obtained in step a and dissolve them in 50 ml of water, and extract them with water to obtain an aqueous solution; the extraction conditions are: shaking at 75°C for 30 hours, collecting the filtrate, and adjusting the pH to 2-3.

[0038] c. The filtrate was sequentially passed through an ion exchange column filled with 732 resin and HPD-100 resin for adsorption at a temperature of 20°C and a filtrate flow rate of 1 Bv / h. After adsorption by the 732 resin, the filtrate was eluted with 2% ammonia water. After adsorption by the HPD-100 resin, the filtrate was eluted with 70% ethanol, and the eluates were combined. The eluates were spray-dried. The main process parameters were: inlet air temperature: 165°C; outlet air temperature: 83°C; atomization pressure: 0.24 MPa; feed flow rate: 8 mL / min. The obtained powder was recorded as peanut sprout aqueous extract enrichment D.

[0039] Analysis showed that the peanut sprout water extract obtained in this example had a resveratrol content of 0.8 mg / g, a total amino acid content of 170 mg / g, a tryptophan content of 0.24 mg / g, and a molar ratio of tryptophan to magnesium of 1:0.54.

[0040] Comparative Example 1 A method for preparing a peanut sprout water extract concentrate comprises the following steps: a. Prepare peanut sprouts: Soak the peanuts in water for 12 hours, then incubate them in a dark environment at 25°C and 89% humidity for 6 days. Cut off the roots of the peanut sprouts and crush them into powder.

[0041] b. Take 10 g of the peanut sprouts obtained in step a and dissolve them in 50 ml of water, and extract them with water to obtain an aqueous solution; the water extraction conditions are: shaking at 60°C for 48 hours, and collecting the filtrate.

[0042] c. Centrifuge the filtrate at 8000 rpm for 10 minutes and collect the supernatant. Repeat the extraction of the residue once and combine the two supernatants.

[0043] d. The obtained supernatant was rotary concentrated at 95°C under reduced pressure to obtain a solid. The powder was dried and recorded as peanut sprout water extract enrichment A-1.

[0044] Analysis showed that the peanut sprout water extract concentrate obtained in this comparative example had a resveratrol content of 0.7 mg / g, a total amino acid content of 90 mg / g, a tryptophan content of 0.01 mg / g, and a molar ratio of tryptophan to metallic magnesium of 1:0.23.

[0045] Comparative Example 2 Peanut sprout aqueous extract concentrate A-2 was prepared in substantially the same manner as in Example 1, except that, in step c, the filtrate was adsorbed only by passing it through an ion exchange column filled with HPD-100 resin; After analysis, the peanut sprout water extract obtained in this comparative example contained 0.9 mg / g of resveratrol, 15 mg / g of total amino acids, and no detectable tryptophan content. out .

[0046] Application Examples First, different sleep-aiding compositions were prepared according to the formula in Table 1, and then granulated and pelletized to obtain pills. Each pill contained 100 mg of peanut sprout aqueous extract.

[0047] Table 1. Application example formula (g)

[0048] The sleep-aiding compositions prepared in each application example and comparative application example were subjected to a sodium pentobarbital-induced sleep experiment, and the process was as follows: (1) Establishment of insomnia mouse model: ICR mice were randomly exposed to one stressor per day for 14 consecutive days, including tail suspension (1 minute), cold water swimming (4°C water, 5 minutes), fasting (24 hours), water deprivation (24 hours), day and night reversal (24 hours), wet pad (24 hours), cage tilted 45° (24 hours) and limb restraint (24 hours). The same stressor was not applied at any time for 2 consecutive days. On days 15-16, 300 mg / kg p-chlorophenylalanine (PCPA) was injected intraperitoneally into the mice once a day to establish an insomnia mouse model. These mice were randomly divided into model group, positive drug group, pill 1 high-dose group, pill 1 medium-dose group, pill 1 low-dose group, pill 2 medium-dose group, pill 3 medium-dose group, pill 4 medium-dose group, pill 1-1 medium-dose group, and pill 1-2 medium-dose group; mice injected intraperitoneally with an equal volume of normal saline were recorded as the control group.

[0049] (2) Medication: Each group of mice was gavaged at the same time every day in the following manner: the high-dose group of Pill 1 was given 50 mg / kg body weight of Pill 1 every day, the medium-dose group of Pill 1 was given 25 mg / kg body weight of Pill 1 every day, and the low-dose group of Pill 1 was given 12.5 mg / kg body weight of Pill 1 every day; the medium-dose group of Pill 2, the medium-dose group of Pill 3, the medium-dose group of Pill 4, the medium-dose group of Pill 1-1, and the medium-dose group of Pill 1-2 were given 25 mg / kg body weight of the corresponding pill every day; the positive drug group was given 2.5 mg / kg body weight of diazepam every day; the model group and the control group were both given an equal volume of normal saline every day.

[0050] (3) Drug efficacy test: On the 10th day of administration, one hour after the end of oral gavage, sodium pentobarbital (55 mg / kg) was injected intraperitoneally, and the time it took for the righting reflex to disappear and recover was recorded for each mouse. The sleep latency was the time from the injection of sodium pentobarbital to the disappearance of the righting reflex, and the sleep duration was calculated as the time interval between the disappearance and recovery of the righting reflex. The results are shown in Table 2.

[0051] Table 2. Experimental results

[0052] From the above test results, it can be seen that the peanut sprout water extract enrichment pills provided by the present invention significantly shortened the sleep latency and prolonged the total sleep time in mice showing sleep disorders. Animal experiments have shown that it can achieve an effect on improving sleep disorders that is equivalent to or even better than the positive drug diazepam, and is expected to be developed into a new sleep-aid drug.

[0053] The above is only a preferred embodiment of the present invention. It should be pointed out that ordinary technicians in this field can make several improvements and supplements without departing from the method of the present invention. These improvements and supplements should also be considered within the scope of protection of the present invention.

Claims

1. A peanut sprout water extract concentrate, characterized in that: The resveratrol content is 0.8-1.5 mg / g, the total amino acid content is 100-200 mg / g, the tryptophan content is 0.24-0.30 mg / g, and the molar ratio of tryptophan to metallic magnesium is 1:(0.4-0.6).

2. A method for preparing the peanut sprout water extract concentrate according to claim 1, characterized in that: The following steps are involved: 1) Dissolve peanut sprouts in water and extract to obtain an aqueous solution; 2) The aqueous solution is passed through an ion exchange column for adsorption, and then eluted with an eluent to obtain an extract; the extract is spray-dried to obtain a powder, which is the peanut sprout water extract concentrate; The ion exchange column is a series connection of a strongly acidic cation exchange resin and a macroporous adsorption resin, preferably a series connection of a 732 resin and a HPD-100 resin.

3. The method for preparing the peanut sprout water extract according to claim 2, wherein: The peanut sprout preparation method comprises the following steps: soaking the peanuts in water for 8-48 hours, then cultivating the peanuts in an environment of 20-28° C. and 85-92% humidity for 5-6 days; and cutting off the roots of the peanut sprouts and crushing the peanuts.

4. The method for preparing the peanut sprout water extract concentrate according to claim 2 or 3, wherein: The water extraction conditions in step 1) are: shaking at 60-80°C for 12-48 hours, and collecting the filtrate.

5. The method for preparing the peanut sprout water extract according to any one of claims 2 to 4, characterized in that: The adsorption conditions in step 2) are: adsorption temperature 20-40°C, preferably 25-35°C, and filtrate flow rate 1-3 Bv / h.

6. The method for preparing the peanut sprout water extract according to any one of claims 2 to 5, characterized in that: After adsorption by the 732 resin, elution is performed using 1-3% ammonia water, and the eluate containing amino acids and magnesium ions is collected; Preferably, after adsorption on the HPD-100 resin, elution is performed using 60-80% ethanol, and the eluate containing resveratrol is collected.

7. The method for preparing the peanut sprout water extract concentrate according to any one of claims 2 to 6, characterized in that: The main process parameters of the spray drying are: inlet air temperature: 160-180°C; outlet air temperature: 80-90°C; atomization pressure: 0.2-0.4 MPa; feed flow rate: 5-10 mL / min.

8. A sleep-aiding composition, characterized in that: Comprising honey and peanut sprout water extract concentrate; the peanut sprout water extract concentrate is the peanut sprout water extract concentrate according to claim 1 or the peanut sprout water extract concentrate prepared by the method according to any one of claims 2 to 7; The mass ratio of the peanut sprout water extract concentrate to honey is 1:(10-20).

9. The sleep-aiding composition according to claim 8, characterized in that: The honey is selected from at least one of locust flower honey, jujube flower honey or vitex honey.

10. Use of the sleep-aiding composition according to claim 8 or 9 in sleep-aiding functional foods or dietary supplements.

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